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<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICJ4</accession>
    <name>B7ICJ4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580111.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1276"/>
    <dbReference type="EnsemblBacteria" id="ACJ75721">
        <property type="protein sequence ID" value="ACJ75721"/>
        <property type="gene ID" value="THA_1276"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1276"/>
    <dbReference type="HOGENOM" id="CLU_2453615_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="89" mass="10239" checksum="B27180E2621C8C8F" modified="2009-02-10" version="1">MASFKQYFITHFFKSVILGLVILFGIVSLIVYIIMKQANEKVQSVSSNFVKVSIELLNDTVSEVSYNFERKIDETIFNIKNNPENLNCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICK1</accession>
    <name>B7ICK1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1283</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580117.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1283"/>
    <dbReference type="EnsemblBacteria" id="ACJ75728">
        <property type="protein sequence ID" value="ACJ75728"/>
        <property type="gene ID" value="THA_1283"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1283"/>
    <dbReference type="eggNOG" id="ENOG5033MD1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121853_0_0_0"/>
    <dbReference type="OMA" id="KFRYMSA"/>
    <dbReference type="OrthoDB" id="1934287at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="176" mass="20672" checksum="CF5C96A353C9ACC3" modified="2009-02-10" version="1">MGIIEWLVLISTISTLSFSWAVYLINLHNSKNLPEKIKEDEERLIQLMGRVRIFVDSKIEILEVKMKELNDLISQVNDLYSKLVLEVSEYQKNIKTNSKISYEEKKEVEANSENFEKTLEETIEKRETKKTEMTREEQIIELYNQGIEEAEIAKKFGMGIGEVRLIIDLFHRTKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICL6</accession>
    <name>B7ICL6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable outer membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580129.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1298"/>
    <dbReference type="EnsemblBacteria" id="ACJ75743">
        <property type="protein sequence ID" value="ACJ75743"/>
        <property type="gene ID" value="THA_1298"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1298"/>
    <dbReference type="eggNOG" id="COG2834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074356_0_0_0"/>
    <dbReference type="OMA" id="NKSGPFM"/>
    <dbReference type="OrthoDB" id="963283at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033399">
        <property type="entry name" value="Put_LolA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17131">
        <property type="entry name" value="LolA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857337" evidence="1">
        <location>
            <begin position="20"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="252" mass="29606" checksum="E1D583F669EFD05A" modified="2009-02-10" version="1">MRKVWAVVSILVFSIFAFSLTGQQVLDMVKDNYQNVKDEKAVFTLKMTDEEGTVKEKQFVIYLYKKSEDDVYAIIRFEKPVSDKRLTLLVKGADNIYLYMPAFRTTKRISGAAKNDRFAGSDFTYKDIELVYKVSDKNYKAELVKEDEKYYYLHITHNDTELDFKELNMKIDKELKIPVYIEFFNWQGEKYKTITFEKIENIQGYNVPTKILANNIKENTKTEIILNDVEFDIGIPESFFSPMTISKPILKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICM1</accession>
    <name>B7ICM1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Chromate transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1303</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chromate ion transporter (CHR) (TC 2.A.51) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580134.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1303"/>
    <dbReference type="EnsemblBacteria" id="ACJ75748">
        <property type="protein sequence ID" value="ACJ75748"/>
        <property type="gene ID" value="THA_1303"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1303"/>
    <dbReference type="eggNOG" id="COG2059">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018106_1_2_0"/>
    <dbReference type="OMA" id="MYAISQA"/>
    <dbReference type="OrthoDB" id="1884249at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015109">
        <property type="term" value="F:chromate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003370">
        <property type="entry name" value="Chromate_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02417">
        <property type="entry name" value="Chromate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="163" mass="18164" checksum="82FCBE5B38AC4E87" modified="2009-02-10" version="1">MLRVFLEFVKIGFIAFGGGWSIIGIIKDAVLSNNWLTPEQFKEILSISQMTPGPVMLNTATYVGLKMYGITGAILNSIAVLVAPITFTTLFFIVRDKLPKEFITALKFGVIFLISLTFLSLLTNISSFYQILISLVAFLLFTKTKIDPIYVIFISGIIGFFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICM7</accession>
    <name>B7ICM7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma factor SigA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">sigA</name>
        <name evidence="9" type="ordered locus">THA_1309</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts transiently with the RNA polymerase catalytic core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the sigma-70 factor family. RpoD/SigA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580140.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1309"/>
    <dbReference type="EnsemblBacteria" id="ACJ75754">
        <property type="protein sequence ID" value="ACJ75754"/>
        <property type="gene ID" value="THA_1309"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1309"/>
    <dbReference type="eggNOG" id="COG0568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_3_3_0"/>
    <dbReference type="OMA" id="YQRWLAE"/>
    <dbReference type="OrthoDB" id="1565734at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00963">
        <property type="entry name" value="Sigma70_RpoD_SigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009042">
        <property type="entry name" value="RNA_pol_sigma70_r1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007127">
        <property type="entry name" value="RNA_pol_sigma_70_r1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042189">
        <property type="entry name" value="RNA_pol_sigma_70_r1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012760">
        <property type="entry name" value="RNA_pol_sigma_RpoD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028630">
        <property type="entry name" value="Sigma70_RpoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03979">
        <property type="entry name" value="Sigma70_r1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00140">
        <property type="entry name" value="Sigma70_r1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02393">
        <property type="entry name" value="RpoD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA_pol_sigma70" evidence="7">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_sigma70" evidence="8">
        <location>
            <begin position="326"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="6">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-2" evidence="6">
        <location>
            <begin position="133"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-3" evidence="6">
        <location>
            <begin position="212"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-4" evidence="6">
        <location>
            <begin position="301"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Interaction with polymerase core subunit RpoC" evidence="6">
        <location>
            <begin position="157"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="371" mass="42788" checksum="0AA6FC9A89E8C2C1" modified="2009-02-10" version="1">MAKKKTVDSLDSKIEKLIAIGKEKGFVTYEDIDKMFPPEKADEFDGNVLEKVYEELEKNNVAITEETTNDGIEDVTLEDFLEESPKFYDNMATKDLIKMYLKDIGKIPLLSQSMERELARRAQLGDERAKQKLVESNLRLVVSIAKRYVGKGLSFLDLIQEGNVGLLKAVEKFDWKKGYKFSTYATWWIRQAITRAIADQARTIRVPVHMVETINKVQKIMREYYQEHGTEIPIEELAKLIGKPVEKVEEILQAAKETTSLEAPVGEDDDSTVGDFIADETIASPKKEAMRMLIREEVDKVLETLNDREKLVLKMRYGLIDGKAKTLEEVGQYFNVTRERIRQIEVKALRKLRHPSRSRYLKLLFKMLDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICQ1</accession>
    <name>B7ICQ1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GufA protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1333</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1333"/>
    <dbReference type="EnsemblBacteria" id="ACJ75778">
        <property type="protein sequence ID" value="ACJ75778"/>
        <property type="gene ID" value="THA_1333"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1333"/>
    <dbReference type="eggNOG" id="COG0428">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015114_1_2_0"/>
    <dbReference type="OMA" id="MIFVVIE"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="246" mass="25917" checksum="C58319C5E5365CBB" modified="2009-02-10" version="1">MNLFLKGILLSSAAGMATSLGAIPFLLFRKGASEKFIDALLGMAAGIMLAASAFSLVAPSIEMGGLLRFGVGFFLGAILVDLMDKYSPHEHFLKGHEGAELKRLSKIWLFVIAITIHNLPEGMAVGVSAFSDQAINIAFAIGAQNIPEGAAVAAALLNAGYSIKTSFWVAFLTGVVEIIGGILGSGIVSISRALLPYMMAFAGGAMIFVISDEVIPETHLRGNERLSTYFLIIGFFIMSALDVVLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICU2</accession>
    <name>Y1374_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoid-associated protein THA_1374</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">THA_1374</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to DNA and alters its conformation. May be involved in regulation of gene expression, nucleoid organization and DNA protection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YbaB/EbfC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101751.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ICU2"/>
    <dbReference type="SMR" id="B7ICU2"/>
    <dbReference type="STRING" id="484019.THA_1374"/>
    <dbReference type="EnsemblBacteria" id="ACJ75819">
        <property type="protein sequence ID" value="ACJ75819"/>
        <property type="gene ID" value="THA_1374"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1374"/>
    <dbReference type="eggNOG" id="COG0718">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140930_1_0_0"/>
    <dbReference type="OMA" id="MGNMMKQ"/>
    <dbReference type="OrthoDB" id="1883809at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043590">
        <property type="term" value="C:bacterial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1310.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00274">
        <property type="entry name" value="DNA_YbaB_EbfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036894">
        <property type="entry name" value="YbaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004401">
        <property type="entry name" value="YbaB/EbfC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02575">
        <property type="entry name" value="YbaB_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004555">
        <property type="entry name" value="UCP004555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82607">
        <property type="entry name" value="SSF82607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000119329" description="Nucleoid-associated protein THA_1374">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00274"/>
        </source>
    </evidence>
    <sequence length="113" mass="12614" checksum="9188A2D9EAEDA8CD" modified="2009-02-10" version="1">MKKLKSFGGKNLSGKSMNQLQKLQEEMQKKLQEVEEGFSNVEVEVSVGGGAIKILATADRKVKDIEIDEDLLSDGETLKDLLTAGINELMEKIEKIREEEMAKVTQNLLPFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICW8</accession>
    <name>B7ICW8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Spermidine/putrescine import ATP-binding protein PotA</fullName>
            <ecNumber evidence="8">7.6.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">potA</name>
        <name evidence="10" type="ordered locus">THA_1401</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD).</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.11" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580206.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1401"/>
    <dbReference type="EnsemblBacteria" id="ACJ75845">
        <property type="protein sequence ID" value="ACJ75845"/>
        <property type="gene ID" value="THA_1401"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1401"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_0"/>
    <dbReference type="OMA" id="HLGWEIQ"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015594">
        <property type="term" value="F:ABC-type putrescine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03300">
        <property type="entry name" value="ABC_PotA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017879">
        <property type="entry name" value="PotA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005893">
        <property type="entry name" value="Sp_pt_ABC_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01187">
        <property type="entry name" value="potA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="6"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="363" mass="41523" checksum="B39FDE46B57662C5" modified="2009-02-10" version="1">MIGSHVSIKGINKWFGDFQVLKNVNLEVKKGEFFSLLGPSGCGKTTLLRIIAGLENADEGDIKFDNVSILSIPPHKRPVNTIFQNYALFPHLNVFENIAFSLRLKKYSENEIKEKVEKLLNLIRLEEHAYKKPNQLSGGQRQRVAIARALANEPKVLLLDEPVSALDAKLRQELLIELDNLHDQVGITFIYVTHDQAEAISISDHVAVMNEGKIVQYGTPYEIYESPADKFVATFIGETNLMKAIVLEINEELLKIEAPGIGEFYCYKDKEVKEKNQILLTLRPEKIKISKKPLTGKNVFHGIIEEEIYMGYQTRYFVKIDNGFILRVYKQHVNYLLDEPILTWKDEVFVSWDPNDSFIVEVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICZ0</accession>
    <name>B7ICZ0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable septum site-determining protein MinC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">minC</name>
        <name evidence="6" type="ordered locus">THA_1423</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Cell division inhibitor that blocks the formation of polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings. Prevents FtsZ polymerization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with MinD and FtsZ.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MinC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101837.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1423"/>
    <dbReference type="EnsemblBacteria" id="ACJ75867">
        <property type="protein sequence ID" value="ACJ75867"/>
        <property type="gene ID" value="THA_1423"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1423"/>
    <dbReference type="eggNOG" id="COG0850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048711_2_1_0"/>
    <dbReference type="OMA" id="MKSFFNI"/>
    <dbReference type="OrthoDB" id="1813503at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901891">
        <property type="term" value="P:regulation of cell septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00267">
        <property type="entry name" value="MinC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013033">
        <property type="entry name" value="MinC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036145">
        <property type="entry name" value="MinC_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005526">
        <property type="entry name" value="Septum_form_inhib_MinC_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34108">
        <property type="entry name" value="PTHR34108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03775">
        <property type="entry name" value="MinC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63848">
        <property type="entry name" value="SSF63848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0717">Septation</keyword>
    <feature type="domain" description="MinC_C" evidence="5">
        <location>
            <begin position="100"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="202" mass="23496" checksum="0960564A9177597C" modified="2009-02-10" version="1">MFNLKIFKSGIVFYIDRYDKLETLFKEIDNKMHDIRQFFDDSEKIMIKIENFKEKIHDIPKIIEKFESYNLKVKGILTEHYDEKETPIKREEDKETTLIIIKNIRSGQKLNHTGHLIIVGNVHSGSEITANGSIVIFGQCSGIVRAGIKIKPSYILALSMNTPLVQIGEVKHQVNNNYKNPVFIYEKGGKLFFDEFKKEEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID04</accession>
    <name>B7ID04_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1437</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580223.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75881">
        <property type="protein sequence ID" value="ACJ75881"/>
        <property type="gene ID" value="THA_1437"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1437"/>
    <dbReference type="eggNOG" id="COG1277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097947_0_0_0"/>
    <dbReference type="OMA" id="SQWFGKN"/>
    <dbReference type="OrthoDB" id="1493141at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032688">
        <property type="entry name" value="ABC2_membrane_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="176" mass="20936" checksum="0A7855F0FC96D183" modified="2009-02-10" version="1">MKREWLQMKVRVISLFILFTILFFSLAPFQNFTINILNENSEAIKKFVGENFVEKLKNWDYYILSQWFGKNFGQFIPILAIIIAFPLFSREYENETITFLLSRQNRKTIFLQKTLLSIFVLLILITYFSYLPSIYSLITSKELSILTVSKFYIHSLIGSFFWFSIALVFTTYFTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID06</accession>
    <name>B7ID06_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetyl-CoA acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1440</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101869.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1440"/>
    <dbReference type="EnsemblBacteria" id="ACJ75883">
        <property type="protein sequence ID" value="ACJ75883"/>
        <property type="gene ID" value="THA_1440"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1440"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_0_0"/>
    <dbReference type="OMA" id="TNVCCTT"/>
    <dbReference type="OrthoDB" id="1058688at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="4">
        <location>
            <begin position="2"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="5">
        <location>
            <begin position="268"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="2">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="400" mass="42925" checksum="C27595743C2AD13F" modified="2009-02-10" version="1">MVYIIGAKRTAIGTFAGSLKDIPAPRLGAIAAKAAIKQAGIEPSDIDETIIGSILTAGQGMGPGRQVGIYSGVPVEKPGFTVNMLCGSGMKATMLGAVDIMQKEAEVVLTGGIENMSASPYLLPARARYGLKFGNFEMIDHMILDGLTDVFNNVHMGITAENLAEKYNISREEQDKFAYESQMKAKAAIESGKFKDEIEKVEIETKKGTIIFDTDEHPRFDVTLEKLAKLKPAFKKDGTVTAGNASGINDGGSAILLASEKYIEKKGLKPLARIVAWAQAGVDPMEMGLGPVPATEKVLQKAGMKMEDIELIELNEAFAAQSLAVIKEWSKRFGVSEEWIKERTNVNGGAIALGHPIGTSGNRIIVTLLYEMKKRNLKYGLATLCIGGGMGTAVIIENIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID07</accession>
    <name>B7ID07_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acetate CoA-transferase subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1441</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 3-oxoacid CoA-transferase subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101871.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1441"/>
    <dbReference type="EnsemblBacteria" id="ACJ75884">
        <property type="protein sequence ID" value="ACJ75884"/>
        <property type="gene ID" value="THA_1441"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1441"/>
    <dbReference type="eggNOG" id="COG2057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019942_4_1_0"/>
    <dbReference type="OMA" id="VAMMHTN"/>
    <dbReference type="OrthoDB" id="1380130at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012791">
        <property type="entry name" value="3-oxoacid_CoA-transf_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004164">
        <property type="entry name" value="CoA_transf_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="PTHR13707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02428">
        <property type="entry name" value="pcaJ_scoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01274">
        <property type="entry name" value="COA_TRANSF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="214" mass="22832" checksum="9CD16E9F6E199B3E" modified="2009-02-10" version="1">MNPKEKIAIRVAKELKEGQLVNLGIGLPTLVANYIPENVHVFFQSENGIVGMGPAPESGFENKDLTNAGGQFVTALPGAMTFDSAFSFALIRGGHLDVTVLGGLQVDEEGHLANWMIPGKMIPGMGGAMDLVTGAKKVIVAMTHTAKGTPKIVKKCSLPLTSIRRVDLIVTELAVIQPTDNGLLLKEIAKETTLDEVLSLTEAKLIISDELKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID28</accession>
    <name>B7ID28_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6 7">Protein RecA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Recombinase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 11" type="primary">recA</name>
        <name evidence="11" type="ordered locus">THA_1462</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8">Belongs to the RecA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101931.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1462"/>
    <dbReference type="EnsemblBacteria" id="ACJ75905">
        <property type="protein sequence ID" value="ACJ75905"/>
        <property type="gene ID" value="THA_1462"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1462"/>
    <dbReference type="eggNOG" id="COG0468">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040469_1_2_0"/>
    <dbReference type="OMA" id="DYGEQAL"/>
    <dbReference type="OrthoDB" id="1080436at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00983">
        <property type="entry name" value="recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00268">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013765">
        <property type="entry name" value="DNA_recomb/repair_RecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020584">
        <property type="entry name" value="DNA_recomb/repair_RecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023400">
        <property type="entry name" value="RecA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45900">
        <property type="entry name" value="PTHR45900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00154">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00142">
        <property type="entry name" value="RECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54752">
        <property type="entry name" value="SSF54752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02012">
        <property type="entry name" value="tigrfam_recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00321">
        <property type="entry name" value="RECA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="RECA_2" evidence="9">
        <location>
            <begin position="39"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="RECA_3" evidence="10">
        <location>
            <begin position="203"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="69"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="353" mass="38304" checksum="FBCEB50E79A49F1A" modified="2009-02-10" version="1">MSNDNAKKREVLKKAIGKIEKTYGKGSIMFLGEESSIQNIETIPSGSVAIDIASGVGGYPRGRIIEIYGPESSGKTTIALHAIASAQKNGGVAAFIDAEHALDPVYAQNLGVNVNDLLIAQPDYGEQALEIVDELVRSNAVDIVVIDSVAALVPRAEIEGSMGDVQVGLQARLMSQALRKLAGNINKSKTVVIFINQTRMKIGVMYGNPETTTGGVALKFYATMRLEVRGIGKITEGSESVGNQVRVKFVKNKVAPPFKEAIVDIIYGKGIVRENELFNIAVDEQLITRRGSWFSYVDLKGVEHSLGQGKNNSIAYLLEHPELLDEIEGIIREKHGLIKKGEEKADEKKESDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID43</accession>
    <name>B7ID43_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine acetyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1477</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>acetyl-CoA + L-serine = CoA + O-acetyl-L-serine</text>
            <dbReference type="Rhea" id="RHEA:24560"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.3.1.30"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.30" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101957.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1477"/>
    <dbReference type="EnsemblBacteria" id="ACJ75920">
        <property type="protein sequence ID" value="ACJ75920"/>
        <property type="gene ID" value="THA_1477"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1477"/>
    <dbReference type="eggNOG" id="COG1045">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_10_1_0"/>
    <dbReference type="OMA" id="ANCIVLE"/>
    <dbReference type="OrthoDB" id="1639008at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03354">
        <property type="entry name" value="LbH_SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045304">
        <property type="entry name" value="LbH_SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042122">
        <property type="entry name" value="Ser_AcTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005881">
        <property type="entry name" value="Ser_O-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000441">
        <property type="entry name" value="CysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="189" mass="20932" checksum="637F250F3C2EA93E" modified="2009-02-10" version="1">MRNFFKDLKNTFIAIKKDKEEILKKDPSIENSYQVLFHVGFISLTFYRISHLFYINNLKFISFLIHFLSRILFSIDIHPAAKIEPGVVIDHGIGTVIGSTASIGEGTIIYHGVTLGARNIQKGKRHPDVGKNVLIGAGAKILGPVKIGNNAKIGANSVVLDDVPENSVFVGIPAKNINEIKKEEIEWII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID61</accession>
    <name>B7ID61_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1497</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101998.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1497"/>
    <dbReference type="EnsemblBacteria" id="ACJ75938">
        <property type="protein sequence ID" value="ACJ75938"/>
        <property type="gene ID" value="THA_1497"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1497"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1420834_0_0_0"/>
    <dbReference type="OrthoDB" id="1485504at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="191" mass="22471" checksum="B6D1E0EF1C1FE5F3" modified="2009-02-10" version="1">MQTFDDSRALDFIKHNEWEWQKVQKIKFKGKSLASGIERILWFCPKCHAFKSISSNGNKAICKNCNSSFIVDEFGYINNQTVEKILKEQVEILDKRFKEINTIKNVKIIIRDKKTNKLHAIKKGDLLISNAELSIKDLYLEFSHIKGVTTFLKKFTEFIYNSNYVVRIKSENESLLLYHILRRYLHVYSNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID98</accession>
    <name>B7ID98_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fibronectin type III domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1534</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102071.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75975">
        <property type="protein sequence ID" value="ACJ75975"/>
        <property type="gene ID" value="THA_1534"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1534"/>
    <dbReference type="HOGENOM" id="CLU_1371632_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="SSF49265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="199" mass="22697" checksum="BD6BE2B4EA9C8D2E" modified="2009-02-10" version="1">MKFKIILAILAIVIIFNSCVLYSEHPEVKVSIQNINYNSVTLTWTYSDNIFRLTKITLKTHENEYLTTFYATDTLAIENLNPGDKYFLIFSAVNESIASTAMYLTTPRLNDTVPPTLTNINVSTVQAQVDVIDVPSGIKEVYIVITNGLEYYKFNMNYKYPNHWSINYALPHRGVWNWEIKCIDKSLNIASANGKITVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDD8</accession>
    <name>B7IDD8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, LacI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1577</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102311.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1577"/>
    <dbReference type="EnsemblBacteria" id="ACJ76015">
        <property type="protein sequence ID" value="ACJ76015"/>
        <property type="gene ID" value="THA_1577"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1577"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_0_0"/>
    <dbReference type="OMA" id="RGCTQAL"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001761">
        <property type="entry name" value="Peripla_BP/Lac1_sug-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00532">
        <property type="entry name" value="Peripla_BP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00036">
        <property type="entry name" value="HTHLACI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="332" mass="37497" checksum="F13B733F4E52B2A7" modified="2009-02-10" version="1">MKQKKYVTIKDIAKVAGVSVNTVSRALNDKPDINSETKKRILDIAKSLGYVKNITASTLRNNSTKIVGVILADSANPFYAEVLKGIEAASRKYGYQIILMNTERVYENEEIAIKTLLQRRVDGLLIAPVQDRAEDIKRLNEKVNIPFVIVGRHFEDIKVDEIYNDEIKGGYIATKYLIDLGRKNILMINGSMFKSPAKMRYEGYKKALNEVGLIDNMVISDIDINGGYEAVKKILKEGQKVDALFCYNDLVAIGAIKALKEEGIKIPEDVAVIGYDDIYFSSFVCPTLTTVRIKKFELGYESFKMLHNRICGRRKNIKQKILDVELIKRESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDH1</accession>
    <name>B7IDH1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Flagellar protein export ATPase FliI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fliI</name>
        <name evidence="6" type="ordered locus">THA_1610</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580296.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1610"/>
    <dbReference type="EnsemblBacteria" id="ACJ76048">
        <property type="protein sequence ID" value="ACJ76048"/>
        <property type="gene ID" value="THA_1610"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1610"/>
    <dbReference type="eggNOG" id="COG1157">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022398_5_1_0"/>
    <dbReference type="OMA" id="MLMMDSV"/>
    <dbReference type="OrthoDB" id="875807at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030257">
        <property type="term" value="C:type III protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030254">
        <property type="term" value="P:protein secretion by the type III secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005714">
        <property type="entry name" value="ATPase_T3SS_FliI/YscN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022425">
        <property type="entry name" value="FliI_clade2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040627">
        <property type="entry name" value="T3SS_ATPase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18269">
        <property type="entry name" value="T3SS_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03497">
        <property type="entry name" value="FliI_clade2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01026">
        <property type="entry name" value="fliI_yscN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA" evidence="5">
        <location>
            <begin position="154"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="434" mass="47787" checksum="CE995C81D7E518FE" modified="2009-02-10" version="1">MNRLELLKKRFENFNPYQKIGSVSKIVGLTIESKGPDAFLGELCKIITDYKKTLAEVVGFNDNGIILMPLEDISGLKKGCQVLKTGEYVSVPVSEELLGKVIDSLGRPLDGKKIYTKEKVPIVREAPNPLVRKRITTPISVGVRAIDGFLTLGYGQRIGIFAGSGVGKSTLLGMVARNTSADINVISLIGERGREVREFIEKDLKKEGLKRSVVVVSTSDQPALLRVKALLTATSIAEYFRDKGYNVLLMVDSLTRWAIAQREVGLATGEPPTTRGYPPSVFAGLPKILERAGNSDKGSITGIYTVLVESDDFNEPISDTVRGIVDGHIILSRKLAESAHYPAIDILMSVSRLMNDITRKEHKIAALKIKDLLATYYDAKDLIDVGAYKKGTNPKIDKAIELIDEINKFLRQSVDEKMSFEDTVEYLISIANKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDI5</accession>
    <name>B7IDI5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1624</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580303.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1624"/>
    <dbReference type="EnsemblBacteria" id="ACJ76062">
        <property type="protein sequence ID" value="ACJ76062"/>
        <property type="gene ID" value="THA_1624"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1624"/>
    <dbReference type="eggNOG" id="ENOG502ZF83">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1342716_0_0_0"/>
    <dbReference type="OMA" id="DIFEMEA"/>
    <dbReference type="OrthoDB" id="1406758at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="211" mass="24394" checksum="3A670754EACC1816" modified="2009-02-10" version="1">MSKVEIILVSLLITLFLFALNFILDGKVVEITGEYFSVFSKPFFSLKMRFMNSFENKNECKIIVFDEMKNELLPLSYTEKGVYFYKIESPGIILTDDKKILGIARKTGKFIFVKKWWYDNVEVTVVANNFEVEGFLKEGKLEVFDNVQINNAKIFLSKNTPYGLALIKMGIHLAEIKDGVLKLNLPDIKTKRIYFILNDYIKNNLGGDRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDI8</accession>
    <name>B7IDI8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative epimerase/dehydratase WbiI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1627</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polysaccharide synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580306.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1627"/>
    <dbReference type="EnsemblBacteria" id="ACJ76065">
        <property type="protein sequence ID" value="ACJ76065"/>
        <property type="gene ID" value="THA_1627"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1627"/>
    <dbReference type="eggNOG" id="COG1086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013560_5_2_0"/>
    <dbReference type="OMA" id="KHNTQIN"/>
    <dbReference type="OrthoDB" id="662797at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05237">
        <property type="entry name" value="UDP_invert_4-6DH_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003869">
        <property type="entry name" value="Polysac_CapD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02719">
        <property type="entry name" value="Polysacc_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_2" evidence="3">
        <location>
            <begin position="271"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="594" mass="68104" checksum="92412D86EA0162C4" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDJ0</accession>
    <name>B7IDJ0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1629"/>
    <dbReference type="EnsemblBacteria" id="ACJ76067">
        <property type="protein sequence ID" value="ACJ76067"/>
        <property type="gene ID" value="THA_1629"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1629"/>
    <dbReference type="HOGENOM" id="CLU_180672_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="92" mass="9920" checksum="5CB133FFBFC0F06A" modified="2009-02-10" version="1">MRCGYVNGVYLIGIGDFNIIFGIFLIGFLIIGFGIYKLIRSFPKGIAAIIVGSIIVASAFGKIHFDVLEFIVILISSGLIEVGLDLIVKKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDK5</accession>
    <name>B7IDK5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CBS domain pair protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1644</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580322.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1644"/>
    <dbReference type="EnsemblBacteria" id="ACJ76082">
        <property type="protein sequence ID" value="ACJ76082"/>
        <property type="gene ID" value="THA_1644"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1644"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114937_0_0_0"/>
    <dbReference type="OMA" id="FLEHQEP"/>
    <dbReference type="OrthoDB" id="2014094at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="66"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="128"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="200" mass="23025" checksum="D576D5738604078B" modified="2009-02-10" version="1">MNVKKWVLQHFPTVKKDEEIGNALRKMREFSCDYCIVLDENDKFEGVVYKSSIRDSELDEKIDMYVTFPDFYVVEDSNVEEAALMLIENKDQVLPVVNNNAEVIGVLTVQEVLEAFIELSAMDEQGTRIILELEDKPGELKRVIDVLANNKMNILSILTLKDDNKRQVSIKIQCEDPESVANLLEIYNIKYISIIEEEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDK6</accession>
    <name>B7IDK6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1645"/>
    <dbReference type="EnsemblBacteria" id="ACJ76083">
        <property type="protein sequence ID" value="ACJ76083"/>
        <property type="gene ID" value="THA_1645"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1645"/>
    <dbReference type="eggNOG" id="ENOG5033NMZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_885474_0_0_0"/>
    <dbReference type="OMA" id="DYRNGWL"/>
    <dbReference type="OrthoDB" id="1226934at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="SSF50969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="311" mass="36784" checksum="C17C8F739A9E68DA" modified="2009-02-10" version="1">MQKIFLLILLTINTLIFPFFTGLKYTFRNTQFENYIVLTSSIERKVYIYDLETNKLKYLFNNVGVYPAISYTDKNYLFIIDTVGKEIILIDLKKQKKYTKRLEHKPIFYNRINSIIYILDSEGNLFGYDFNLNLVYYHKFLSTPNYFYFYKSKPIGLYIWKNDIDFEYQGKIYNYNLTTPSLLVDNFIIDTRGGNILEIDKKKLYKSEPYISFAIIWNKKLYFGSMFSNTIYSIESGKIEKVAKLNYQPTYAKIINDKLYILSASNNKLIIFDNNTIQEIETGDFPIEIFSYKGEIAVVCAENGEINIFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDL3</accession>
    <name>B7IDL3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">YuiD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580328.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1652"/>
    <dbReference type="EnsemblBacteria" id="ACJ76090">
        <property type="protein sequence ID" value="ACJ76090"/>
        <property type="gene ID" value="THA_1652"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1652"/>
    <dbReference type="eggNOG" id="COG1963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073969_1_1_0"/>
    <dbReference type="OMA" id="GRYGGMP"/>
    <dbReference type="OrthoDB" id="1864362at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003832">
        <property type="entry name" value="DUF212"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31446">
        <property type="entry name" value="PTHR31446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02681">
        <property type="entry name" value="DUF212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="114" mass="12205" checksum="EDC0241728B4BC6E" modified="2009-02-10" version="1">MLQLLSNKALLAAFFAFLSAQILKVIIYRDIKSFGRYGGMPSAHVATTAALAWEVARITGYDSPETAIAAIFLSIVASDAVGLRRKVDPNSGHTLIEAILGFILGTIIAFIIPK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDL6</accession>
    <name>DTD_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-aminoacyl-tRNA deacylase</fullName>
            <shortName evidence="1">DTD</shortName>
            <ecNumber evidence="1">3.1.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Gly-tRNA(Ala) deacylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dtd</name>
        <name type="ordered locus">THA_1655</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-tRNA(Ala) + H2O = glycine + H(+) + tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DTD family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102462.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDL6"/>
    <dbReference type="SMR" id="B7IDL6"/>
    <dbReference type="STRING" id="484019.THA_1655"/>
    <dbReference type="PRIDE" id="B7IDL6"/>
    <dbReference type="EnsemblBacteria" id="ACJ76093">
        <property type="protein sequence ID" value="ACJ76093"/>
        <property type="gene ID" value="THA_1655"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1655"/>
    <dbReference type="eggNOG" id="COG1490">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076901_1_0_0"/>
    <dbReference type="OMA" id="GVFQAHM"/>
    <dbReference type="OrthoDB" id="1862614at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043908">
        <property type="term" value="F:Ser(Gly)-tRNA(Ala) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00563">
        <property type="entry name" value="Dtyr_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00518">
        <property type="entry name" value="Deacylase_Dtd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003732">
        <property type="entry name" value="Daa-tRNA_deacyls_DTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472">
        <property type="entry name" value="PTHR10472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02580">
        <property type="entry name" value="Tyr_Deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69500">
        <property type="entry name" value="SSF69500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00256">
        <property type="entry name" value="TIGR00256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_1000127586" description="D-aminoacyl-tRNA deacylase">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Gly-cisPro motif, important for rejection of L-amino acids" evidence="1">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00518"/>
        </source>
    </evidence>
    <sequence length="149" mass="16662" checksum="7125C6E2FD737808" modified="2009-02-10" version="1">MRAVIQRVKNAKVEVEGKTVGKIENGLLVLLGVGKDDNKEDIKYLAEKIINLRIFDDENGKMNLSLLDINGELLIVSQFTLYGDCRKGRRPSYSESAPPDIAKKLYEEFVNACKNYNLKVETGEFGAHMQVSLVNDGPVTLLLDSKKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDR1</accession>
    <name>B7IDR1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">2',3'-cyclic-nucleotide 2'-phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1701</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a nucleoside 2',3'-cyclic phosphate + H2O = a nucleoside 3'-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19621"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:66949"/>
            <dbReference type="ChEBI" id="CHEBI:66954"/>
            <dbReference type="EC" id="3.1.4.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 3'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10144"/>
            <dbReference type="ChEBI" id="CHEBI:13197"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.1.3.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the 5'-nucleotidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580360.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1701"/>
    <dbReference type="EnsemblBacteria" id="ACJ76138">
        <property type="protein sequence ID" value="ACJ76138"/>
        <property type="gene ID" value="THA_1701"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1701"/>
    <dbReference type="eggNOG" id="COG0737">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005854_4_4_0"/>
    <dbReference type="OMA" id="EKAQYPM"/>
    <dbReference type="OrthoDB" id="1884225at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008663">
        <property type="term" value="F:2',3'-cyclic-nucleotide 2'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008254">
        <property type="term" value="F:3'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07410">
        <property type="entry name" value="MPP_CpdB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008334">
        <property type="entry name" value="5'-Nucleotdase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006146">
        <property type="entry name" value="5'-Nucleotdase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041827">
        <property type="entry name" value="CpdB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="PTHR11575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02872">
        <property type="entry name" value="5_nucleotid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01607">
        <property type="entry name" value="APYRASEFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="SSF54106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="SSF55816"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00785">
        <property type="entry name" value="5_NUCLEOTIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00786">
        <property type="entry name" value="5_NUCLEOTIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="8">
        <location>
            <begin position="539"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="600" mass="67973" checksum="147841C71CD8C83E" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDR5</accession>
    <name>B7IDR5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1706</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580363.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1706"/>
    <dbReference type="EnsemblBacteria" id="ACJ76142">
        <property type="protein sequence ID" value="ACJ76142"/>
        <property type="gene ID" value="THA_1706"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1706"/>
    <dbReference type="HOGENOM" id="CLU_1593774_0_0_0"/>
    <dbReference type="OMA" id="FNIQWIF"/>
    <dbReference type="OrthoDB" id="2058542at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="174" mass="20088" checksum="B1F0EFE21093ED19" modified="2009-02-10" version="1">MKKIFAILIILTIFSLIFSNETFLDFGIGWNLGKFSNYGLNLGFSNNLFNIQWIFNFSENFDHKIFVDSAIAKFENISFGPILFVNNTTYDSTSLYIEAGLKTSLYIPDIEISLSLTYPLNKEVNYNLTDFLLVNLKYIVPHPNTKKIWRDKLFLLISYSKKTVFFGVGLSEPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDT6</accession>
    <name>B7IDT6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription termination/antitermination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">nusA</name>
        <name evidence="8" type="ordered locus">THA_1727</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Participates in both transcription termination and antitermination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer. Binds directly to the core enzyme of the DNA-dependent RNA polymerase and to nascent RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102602.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1727"/>
    <dbReference type="EnsemblBacteria" id="ACJ76163">
        <property type="protein sequence ID" value="ACJ76163"/>
        <property type="gene ID" value="THA_1727"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1727"/>
    <dbReference type="eggNOG" id="COG0195">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029242_2_2_0"/>
    <dbReference type="OMA" id="MDIDMSA"/>
    <dbReference type="OrthoDB" id="384865at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1480.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_B">
        <property type="entry name" value="NusA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025249">
        <property type="entry name" value="KH_dom_NusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="NusA_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036555">
        <property type="entry name" value="NusA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013735">
        <property type="entry name" value="TF_NusA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010213">
        <property type="entry name" value="Tscrpt_termination_fac_NusA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="PTHR22648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13184">
        <property type="entry name" value="KH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08529">
        <property type="entry name" value="NusA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69705">
        <property type="entry name" value="SSF69705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01953">
        <property type="entry name" value="NusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="4 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="S1" evidence="7">
        <location>
            <begin position="133"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="344" mass="38528" checksum="E3B54E2BF7429AE5" modified="2009-02-10" version="1">MNLDFLQALEQLEEEKGVKVDEIIPVIEKALISAYKKDFGGEKNVEIIINRQTGEIEAYQLLEVVENVEKPNLQISLEEALKINSSAKIGDIVKKRLNIKKFGRIAAQTAKQVLIQKIREIEKEKQFEKYSELAGKITTAEVVRVTKDWADIRIGKLETRLPKKEWIPGEEIQPASLIKVYVKDVKKDKKGPKILVTRINEEFIKGLLELEVPEIESKVVEVVKIVREPGIRTKVAVKSNNPKVDPVGACIGEEGIRIAAILKELNGEKIDIIKWSDDPKELIASALQPANVIEVEILDYENKASRVIVSPNQLSLAIGKGGQNARLAAKLTGWKIDIKPIMNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDY0</accession>
    <name>B7IDY0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Integral membrane protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">THA_1776</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102683.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1776"/>
    <dbReference type="EnsemblBacteria" id="ACJ76207">
        <property type="protein sequence ID" value="ACJ76207"/>
        <property type="gene ID" value="THA_1776"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1776"/>
    <dbReference type="eggNOG" id="COG1253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015237_4_1_0"/>
    <dbReference type="OMA" id="TIGRQHP"/>
    <dbReference type="OrthoDB" id="1155802at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="DUF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="11">
        <location>
            <begin position="3"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="213"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="282"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ76207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="430" mass="49194" checksum="35083E3F766A5C26" modified="2009-02-10" version="1">MEDPLSYLLNLFLLIFLLYLSSLFSASETALTSVSKLKLRESLKKGDKNEEESELHLFNKLLTVILIMNNLVNILASSIATLVVVNAFKNVLPESLSALFSTLILTFFILIFGEITPKIYARQNNEKIFNKMIGILIFLSKIFSPIIKLLVSISNFVIRIIGGTYVEEAPFITTEDIIMYVEVGREEGTIKHEESFMLKRTLEMEESVVREIMIPRVDIVAIEESETLDKVMEIIKEEEYSRIPVYKETIDNVVGICYAKDVLAFISDRGSEICEKVKVKELMREPLFVPETMKVSELLKIFKEQKMHMAIVVDEYGGTAGLVTMEDILEEIFGEIMDEYDQNENLGIKKLENGSYIVDATVPINDIERELKIEFPETDYETLAGYILEHLQRIPKVGEEIIIDKFKFKIIAASKNRIEKVLVKVVKDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE11</accession>
    <name>B7IE11_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1807</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580431.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1807"/>
    <dbReference type="EnsemblBacteria" id="ACJ76238">
        <property type="protein sequence ID" value="ACJ76238"/>
        <property type="gene ID" value="THA_1807"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1807"/>
    <dbReference type="eggNOG" id="COG0864">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170247_0_0_0"/>
    <dbReference type="OMA" id="RVGYPIE"/>
    <dbReference type="OrthoDB" id="1890200at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023860">
        <property type="entry name" value="FeFe-hyd_TM1266"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03959">
        <property type="entry name" value="hyd_TM1266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="82" mass="9426" checksum="58B7711D14266AA8" modified="2009-02-10" version="1">MERFYTVDIIVTNRDDAYENVNQILHDFSRYIKLRVGYPIESENMAVIFVLFRATNDILGSFTGKLGQIKNVKVKSIPITKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE98</accession>
    <name>B7IE98_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal silencing factor RsfS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rsfS</name>
        <name evidence="3" type="ordered locus">THA_1899</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as a ribosomal silencing factor. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8. Prevents association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with ribosomal protein L14 (rplN).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Iojap/RsfS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580473.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1899"/>
    <dbReference type="EnsemblBacteria" id="ACJ76325">
        <property type="protein sequence ID" value="ACJ76325"/>
        <property type="gene ID" value="THA_1899"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1899"/>
    <dbReference type="eggNOG" id="COG0799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092688_6_1_0"/>
    <dbReference type="OMA" id="VVHVFQK"/>
    <dbReference type="OrthoDB" id="1990650at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:mature ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090071">
        <property type="term" value="P:negative regulation of ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01477">
        <property type="entry name" value="Iojap_RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004394">
        <property type="entry name" value="Iojap/RsfS/C7orf30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043">
        <property type="entry name" value="PTHR21043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00090">
        <property type="entry name" value="rsfS_iojap_ybeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="110" mass="12896" checksum="836C6FA22C0646A5" modified="2009-02-10" version="1">MEILELIWKKILEKEAIEPVILDMSNTNVPTDYFIILTANSNTHMNSLRDTILDLLSEIGKQVIYYDKEDNHEWLIIDASDIVIHIFTKEAREFYDLEGLWIDAKKLKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEB3</accession>
    <name>B7IEB3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ubiquinone/menaquinone biosynthesis methyltransferase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1916</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1916"/>
    <dbReference type="EnsemblBacteria" id="ACJ76340">
        <property type="protein sequence ID" value="ACJ76340"/>
        <property type="gene ID" value="THA_1916"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1916"/>
    <dbReference type="eggNOG" id="COG2227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097888_0_0_0"/>
    <dbReference type="OMA" id="YDSMYET"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0830">Ubiquinone</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="200" mass="23512" checksum="FA8D1DCBA4CE1DC3" modified="2009-02-10" version="1">MDKTINMKNARVLDIGSGTGYWLEYFLNKGFSVYALEPSSKMIEFAKERFKDKVKYFNTTIENFVTDNKFDILNIQGDVLSYVENLDLVMKKISNIINKDGLLFATVDSYYYMRKLVKKYGNSREMEIFEKYHITTVGSQYGIFNSKCFTLEDIKELEKYGFQVLEIRGCGYSDNLEEEIEKSYNIVLGEHIYFSLRYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IED7</accession>
    <name>B7IED7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Beta-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1942</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580493.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1942"/>
    <dbReference type="CAZy" id="GH3">
        <property type="family name" value="Glycoside Hydrolase Family 3"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76364">
        <property type="protein sequence ID" value="ACJ76364"/>
        <property type="gene ID" value="THA_1942"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1942"/>
    <dbReference type="eggNOG" id="COG1472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004542_4_1_0"/>
    <dbReference type="OMA" id="VEPAYEF"/>
    <dbReference type="OrthoDB" id="949956at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="SSF52279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fn3_like" evidence="4">
        <location>
            <begin position="632"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="719" mass="81386" checksum="20CD9C18B2CE4C02" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEI6</accession>
    <name>B7IEI6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1992</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76413">
        <property type="protein sequence ID" value="ACJ76413"/>
        <property type="gene ID" value="THA_1992"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1992"/>
    <dbReference type="HOGENOM" id="CLU_3085726_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="52" mass="6125" checksum="84B16D918C46AEDE" modified="2009-02-10" version="1">MIKKVIIIFLIILIFQAFSVQFSNIKPKHEEGPFVEFTSLGYAFKNFEILKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEI7</accession>
    <name>B7IEI7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1993</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103068.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1993"/>
    <dbReference type="EnsemblBacteria" id="ACJ76414">
        <property type="protein sequence ID" value="ACJ76414"/>
        <property type="gene ID" value="THA_1993"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1993"/>
    <dbReference type="eggNOG" id="ENOG5034BG2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1626270_0_0_0"/>
    <dbReference type="OrthoDB" id="1403080at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="163" mass="19137" checksum="75A4A97EAA580777" modified="2009-02-10" version="1">MKESIESTPTYIFATRNYYFSNYNLDRVSIKSYFEMFFPGIADFSDYVFDIVPRGFVNVGYFILDKIEFFGLLEGGVVLNLIISSGTKDSDWDNFIKELRQESIYSTFKFGFSWYYDNYSGIELGYRSFLLGKNSPLRFIQGFTTTDWIYNFVSYTLYTENGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEJ1</accession>
    <name>B7IEJ1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1997</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76418">
        <property type="protein sequence ID" value="ACJ76418"/>
        <property type="gene ID" value="THA_1997"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1997"/>
    <dbReference type="HOGENOM" id="CLU_3141681_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="49" mass="5889" checksum="21B913BE4D194426" modified="2009-02-10" version="1">MKLANNIRNIEKDFQKLNEFKKLHYKNLVEIIIGLESRVRKLSDFNTLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEJ2</accession>
    <name>B7IEJ2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mrr_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1998</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580535.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1998"/>
    <dbReference type="EnsemblBacteria" id="ACJ76419">
        <property type="protein sequence ID" value="ACJ76419"/>
        <property type="gene ID" value="THA_1998"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1998"/>
    <dbReference type="eggNOG" id="COG1715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117611_0_0_0"/>
    <dbReference type="OMA" id="WCRNTLV"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025745">
        <property type="entry name" value="Mrr-like_N_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14338">
        <property type="entry name" value="Mrr_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mrr_N" evidence="2">
        <location>
            <begin position="119"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="230" mass="27698" checksum="68ECC3C89FF74F6A" modified="2009-02-10" version="1">MFLQRKERFYYGINKKELNEFFEILIEEIKNIINELNHEFELENKSNDFNKITKIEERITKFISIIEKLQELKKECDNMFNNIDLKKEWNRIFMVKNSKITSRKKFVKLEKGLKTPEKEFRIPILEALVELGGRAEAKEVLKIVEQKLKGKLNKYDYENIPSNPHKIRWKDTASWCRNTLVNEGLLSSNSPKGVWEITEKGRKYLEDYKKRIENNDEIILFEIKNNKTRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IER0</accession>
    <name>B7IER0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ABC-type thiamine transport system, periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_66</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579326.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_66"/>
    <dbReference type="EnsemblBacteria" id="ACJ74574">
        <property type="protein sequence ID" value="ACJ74574"/>
        <property type="gene ID" value="THA_66"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_66"/>
    <dbReference type="eggNOG" id="COG4143">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026974_6_1_0"/>
    <dbReference type="OMA" id="PTTNWMY"/>
    <dbReference type="OrthoDB" id="1567509at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030975">
        <property type="term" value="F:thiamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015888">
        <property type="term" value="P:thiamine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13545">
        <property type="entry name" value="PBP2_TbpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005948">
        <property type="entry name" value="ThiB-like"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01254">
        <property type="entry name" value="sfuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="325" mass="37271" checksum="68B9E304054AE6E5" modified="2009-02-10" version="1">MKKVLIYLILTISVILFSAEKLVVYTYDSFVSGIGKEIVPIFEKMYGCKVELLSFGDAGTVLSRLMLEKNNPKADVIVGLDQALLIRAIENDLVQKFKPENLSLLKYPELYNEYGTPYDFGAIAIVYNKEKIKNPPKSFNDLLKDEFRGKIVVQDPRTSSTGLSFLLWTIAAFNEKYLDFWSKFKDNLLTITPGWDEAFEMLESGEADIMVSYATDGAYSYYYYGDVKYVPVILDEGAFVQVEYASLVKNAKNEELAKKFIEFLLSDDFQEKVPLNQWMLPVTNVNLPEAFKYVPEIKKVLEIKEDIYMNQERILKEWAKEVIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IER3</accession>
    <name>B7IER3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_69</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103415.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_69"/>
    <dbReference type="EnsemblBacteria" id="ACJ74577">
        <property type="protein sequence ID" value="ACJ74577"/>
        <property type="gene ID" value="THA_69"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_69"/>
    <dbReference type="eggNOG" id="COG1633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065373_1_0_0"/>
    <dbReference type="OMA" id="ADDEMRH"/>
    <dbReference type="OrthoDB" id="745826at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:cellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01044">
        <property type="entry name" value="Ferritin_CCC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039376">
        <property type="entry name" value="Ferritin_CCC1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="PTHR31851"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="286" mass="32902" checksum="D59A8483F21EB049" modified="2009-02-10" version="1">MHKEKYLIQFQKNEITEYYVYKKLASFSKNKTNKNVLIKIADDELKHYYYFKKYTNKELRPNIFKVWWYTFLSFLFGLTFALNAMEKGEESAQKKYKEFVGKVPEINKIINDENVHEKELLSLIDEEKINYISSMVLGLNDALVELTGTLAGLTFAFQNSRMVALSGIITGIAASLSMSASEYLSQKADKNSQNPLKAAIYTGIAYVLTVIFLVIPYLVFSKPIISLSFLIINAIVVILLFSYFVSVVQEKTFKQYFLEMFLISFGVMIVSFIIGIIAKRLFNIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IER8</accession>
    <name>B7IER8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transglycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_74</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579333.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_74"/>
    <dbReference type="CAZy" id="GH23">
        <property type="family name" value="Glycoside Hydrolase Family 23"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ74582">
        <property type="protein sequence ID" value="ACJ74582"/>
        <property type="gene ID" value="THA_74"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_74"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065765_7_0_0"/>
    <dbReference type="OMA" id="YLICAII"/>
    <dbReference type="OrthoDB" id="1444566at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLT" evidence="1">
        <location>
            <begin position="36"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="159" mass="18518" checksum="B0F669F961F085EA" modified="2009-02-10" version="1">MRFFILLISLLIITCIFYLYELFPLKYIDLIPNSSFDKLLILSIIKVESSFKTNAISNVGAYGLMQVLPSTAEWINNKFNTNYNLKVPKDNILLGITYLEYLYNITKDLQKTISYYNTGPNASDEIVKTAGLNYYNKIMKAYSLYKFLYRSDNYEISNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IES7</accession>
    <name>B7IES7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Endoglucanase, aminopeptidase M42 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_83</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103436.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_83"/>
    <dbReference type="MEROPS" id="M42.A02"/>
    <dbReference type="EnsemblBacteria" id="ACJ74591">
        <property type="protein sequence ID" value="ACJ74591"/>
        <property type="gene ID" value="THA_83"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_83"/>
    <dbReference type="eggNOG" id="COG1363">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053520_0_0_0"/>
    <dbReference type="OMA" id="HGMERTH"/>
    <dbReference type="OrthoDB" id="900854at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="343" mass="38019" checksum="7F533CF06BD45DC0" modified="2009-02-10" version="1">MTYVQEAVNKIVELCNIPSPTGYTYKAIKYLIDEFKNLGFKYYLTNKNNLLVDLGHGDSNAIMFTAHVDTLGAMVRSITNKGTLKITRIGGYPFSHIENENCTIFTRDGKEFTGTIYNIHPSVHVYEDSGTIKRSEENMEIVLDQKVHSKEDVIELGIMPGDFVSFDPRTVVTKTGFIKSRHLDDKASAGILIALAKSIRDFAIEPKRRVYMLFTSYEEVGHGGAANIPEDVTEVIAVDMGAVGSDLESNVYSASICAKDSSGPYDYEIVGKLIKAAKFAEVNYTVDIYPYYSSDVSAALRAGHDIKHGLVGPGIYASHGYERTHIEAIEATLKLLQKYLEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEX3</accession>
    <name>B7IEX3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">1-phosphofructokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pfkB</name>
        <name evidence="4" type="ordered locus">THA_129</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579365.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_129"/>
    <dbReference type="EnsemblBacteria" id="ACJ74637">
        <property type="protein sequence ID" value="ACJ74637"/>
        <property type="gene ID" value="THA_129"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_129"/>
    <dbReference type="eggNOG" id="COG1105">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050013_0_2_0"/>
    <dbReference type="OMA" id="GETRSNI"/>
    <dbReference type="OrthoDB" id="1323781at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01164">
        <property type="entry name" value="FruK_PfkB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017583">
        <property type="entry name" value="Tagatose/fructose_Pkinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000535">
        <property type="entry name" value="1PFK/6PFK/LacC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03168">
        <property type="entry name" value="1-PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="3">
        <location>
            <begin position="12"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="307" mass="33977" checksum="F0EEC7BC7BC1E4B3" modified="2009-02-10" version="1">MIFTLTMNPCLDRYIYVDDLKLDDTIRAKKVVDYPAGKGIDVSRVIKELGGISVAISLLGGDTGRKIEEMLDMEGVIYTTIRVSVETRTNIILETNSGQFRISVPTKKVSRKKLETVLEVLNAIVRKGDIIVISGSLPTGVSPDFYTGIIFALKQWGVYVYFDADGEKLKSGLLGGPDVIKPNQHEFERLVGKKIEKFEDFIEQGKKLILEYNLKEILLTLGSKGALLLANDEGYFAKPIDVPVNSAVGAGDSFLAAYVLMRDKGRYEAFKWANAAGNAAVMTPGTELCKKDDVLNLLDRVQIERIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEX5</accession>
    <name>B7IEX5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobalt ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_131</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579366.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_131"/>
    <dbReference type="EnsemblBacteria" id="ACJ74639">
        <property type="protein sequence ID" value="ACJ74639"/>
        <property type="gene ID" value="THA_131"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_131"/>
    <dbReference type="eggNOG" id="COG0619">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056469_2_2_0"/>
    <dbReference type="OMA" id="NNIMIGR"/>
    <dbReference type="OrthoDB" id="1479665at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="247" mass="28661" checksum="82CA28F97AA36568" modified="2009-02-10" version="1">MKFAFGRYVPTDSVIHSLDPRSKIVSSFLFIATVLSVSSLYDYIWLFLLFMVIVKLSKISIKIYLRSVKNMWFLIAFATVIQYFVSGIETAAFIALRLIFVVLFASFLTYTTSPLLIARGLADLLKYFGVKKRYREDFSMIMTISFRFIPILFDEVDRIIKAQIARGAKFDQPGIRYKVQGIVAIIIPLLISAIRKAEEISIALQARKYGVFERSSYYVLKWKKIDTYFITFSLLVFLFLFILKIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF69</accession>
    <name>B7IF69_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sugar ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_228</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579429.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_228"/>
    <dbReference type="EnsemblBacteria" id="ACJ74733">
        <property type="protein sequence ID" value="ACJ74733"/>
        <property type="gene ID" value="THA_228"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_228"/>
    <dbReference type="eggNOG" id="COG3845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_92_0_0"/>
    <dbReference type="OMA" id="VGREMSH"/>
    <dbReference type="OrthoDB" id="551294at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="256"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="507" mass="57254" checksum="2F153DBAD4F3CC5B" modified="2009-02-10" version="1">MYAVEMVNIVKKFPGVLANDHVTIRIKKGEIHAIVGENGAGKSTLMNQLYGLYHPDEGDILINGKKVTIKGPKDAIKNGIGMVHQHFMLVDTLTVAENIVLGSEPVKGLNFDIKEARKQVKELSEKYGLFVDVDAKIEDIPVGMQQRVEILKTLYRGANIIILDEPTAVLTPQEVEELFEIMRKLKSDEKTILFISHKLHEVMEISDRITVMRLGKVTGELETSKTNPKEIARHMVGRDVVLRVEKAPHKPKEKVFEIKDLVVKDNRGLLAVNKVSFDIRKGEIVGIAGVAGNGQTELVEAITGLRKIEDGKIYFEGKDVTHFTPKQLRELGLAHIPEDRLKHGLIEEFEAYYNVILGQHYKPPFSNGTFLNHKEIFEYTKNLMEEFDVRPRRIQHLGGNFSGGNQQKLVVGREIRMNPKFLVVAQPTRGLDVGAIEFIHKQILKMREMDVGILLISMELEEIFSLSDRIIVMYEGEIMGEVKPEETTVEEVGLMMTGQRLVEARRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF72</accession>
    <name>B7IF72_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_231</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579430.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ74736">
        <property type="protein sequence ID" value="ACJ74736"/>
        <property type="gene ID" value="THA_231"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_231"/>
    <dbReference type="HOGENOM" id="CLU_1785975_0_0_0"/>
    <dbReference type="OrthoDB" id="162439at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="145" mass="17197" checksum="55FD3541170B9106" modified="2009-02-10" version="1">MHKYANSFPYYRRWSLLCFYFDKNENISSLKYILASPNIVQDVEFTEDYIILSRSYGINNDSKLEFYPNVLNNKPQKKINNISVWFLDVPEKTINILPMSEGISKIDNSLYILFESGALKYKNFCKSPTEYIWKLNIEILSKKEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF73</accession>
    <name>B7IF73_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable queuosine precursor transporter</fullName>
            <shortName evidence="1">Q precursor transporter</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the import of queuosine (Q) precursors, required for Q precursor salvage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family. Q precursor transporter subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_234"/>
    <dbReference type="EnsemblBacteria" id="ACJ74737">
        <property type="protein sequence ID" value="ACJ74737"/>
        <property type="gene ID" value="THA_234"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_234"/>
    <dbReference type="eggNOG" id="COG1738">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075503_2_1_0"/>
    <dbReference type="OMA" id="IFQKFFY"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990397">
        <property type="term" value="P:queuosine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02088">
        <property type="entry name" value="Q_prec_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003744">
        <property type="entry name" value="YhhQ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34300">
        <property type="entry name" value="PTHR34300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02592">
        <property type="entry name" value="Vut_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00697">
        <property type="entry name" value="TIGR00697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="232" mass="27087" checksum="9A02228EB5CB1888" modified="2009-02-10" version="1">MTNEVLWFLLMLVNFGGILLFYRLMGKTGLYIWTAIATIIANIQVLKTVELFGFVATLGNIVYGTTFLVTDILSENYGKKDARKAVYVGFWSLIVMTVMMQLALYFKPDQSDFAQGALETIFSIMPRIVIASLTAYWLSQLHDIWAYHLWKNKLPQLKFLWVRNNLSTMVSQFIDSFVFCFIAFWGVYELNVFWSILWTTYFFKWIVAAADTPFLYFAKYLHDKNKINELVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF82</accession>
    <name>B7IF82_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aldose 1-epimerase</fullName>
            <ecNumber evidence="3">5.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_243</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>alpha-D-glucose = beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:10264"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="5.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the aldose epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.3" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579438.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_243"/>
    <dbReference type="EnsemblBacteria" id="ACJ74746">
        <property type="protein sequence ID" value="ACJ74746"/>
        <property type="gene ID" value="THA_243"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_243"/>
    <dbReference type="eggNOG" id="COG2017">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031753_1_0_0"/>
    <dbReference type="OMA" id="ATWLSCK"/>
    <dbReference type="OrthoDB" id="1512477at2"/>
    <dbReference type="UniPathway" id="UPA00242"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019318">
        <property type="term" value="P:hexose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015443">
        <property type="entry name" value="Aldose_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005096">
        <property type="entry name" value="GALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="84"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="184"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR005096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="342" mass="38710" checksum="F5BB1BA753F414A2" modified="2009-02-10" version="1">MFGKITKELFGKTQIETPVFQYTLENKNGMIVKALNYGGIITNIWVKDKFGKYVDVVLGFDSLEEYEKLNHKYYFGAVIGRYANRIKNGQFKIDDTIYQLALNDGKNSLHGGIGGFHTKIFKTSTEITPYGPKLNLKYLSHDGEEGFPGNLLVNVIYTLTNNNELIIEYIAQTDKPTVVNLTQHSYFNLSGKGCIYDHKIQINADLYTPVDENLVPTGEIVPVENTPYDFKQPKNLGKMLEKIDYDVNFVLNKSKIATVVSSLETGIKLEVSTTKPGIQLYTGNFLDNIIGKSGNVYKKHSGLCLETQYFPDSPNKKNFPNVILKKGDIYYHKTTFKFTVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFB1</accession>
    <name>B7IFB1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TldD/PmbA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_275</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579462.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_275"/>
    <dbReference type="EnsemblBacteria" id="ACJ74775">
        <property type="protein sequence ID" value="ACJ74775"/>
        <property type="gene ID" value="THA_275"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_275"/>
    <dbReference type="eggNOG" id="COG0312">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_4_0_0"/>
    <dbReference type="OMA" id="LYRKSTF"/>
    <dbReference type="OrthoDB" id="958356at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD" evidence="2">
        <location>
            <begin position="23"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_M" evidence="4">
        <location>
            <begin position="111"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_C" evidence="3">
        <location>
            <begin position="220"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="435" mass="48370" checksum="597C91AF2F614F03" modified="2009-02-10" version="1">MTYNEFKEKLFSIAKEKGFEAQIDLSESSEFSLRYANDDIDQYTDASKFSVSIKILKDGKIGRVYTEQLENPEKLFEEALKNWELTDSEDEEFFYDGTGKYIEMDLYNGSFEKTSVSEKIEFVKKLYNSAKCDDRIVMVPYAMYNNFKVSKKISNTLGLDVSTTADGGYSYAMAVSKDESTHSGLWMEVGTKFSDLDPEKIGKTACQNALSLLGAKSVKSGKYRVIIKNTAFEDMLSLLISMISAENVQKNMSPLKGKQNQKIASTILNIKDVPYYKNSISNRPFDDEGVPTKETPIIENGILKSFLYDLKTAKKDNTEPTGNSLGGSIRPINLLVESGEYDFENLLKKLDDGIVIIGVDGMHSGANPISGNFSLGARGFRVINGEISHPIEQITISGNFLQLLEKVEAVGNDSKIFYGIITPSILISELDIAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFF3</accession>
    <name>B7IFF3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SAVED domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_317"/>
    <dbReference type="EnsemblBacteria" id="ACJ74817">
        <property type="protein sequence ID" value="ACJ74817"/>
        <property type="gene ID" value="THA_317"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_317"/>
    <dbReference type="eggNOG" id="COG1067">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_572057_0_0_0"/>
    <dbReference type="OMA" id="CPVPIAF"/>
    <dbReference type="OrthoDB" id="1402034at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040836">
        <property type="entry name" value="SAVED"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18145">
        <property type="entry name" value="SAVED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAVED" evidence="1">
        <location>
            <begin position="357"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="479" mass="54782" checksum="E30991B58522D203" modified="2009-02-10" version="1">MFSRLLSSNSSDFLKILERDDITVKEAIESFINAEDFDNEFLVFYEIVKKIKEKKIVDLNQELLKKIFNGEEEIIFDIYKNDFVEVFFPTVNKLGNGKIARAIIFESKKSFTNNPDLKSSLEKIESLVGKKLIVIFDSEFKGNSFELAVAIGSLCKKIPKNIAFTGEIDEKGNIKRNIEYLDLKMKICSENNLKLISAFDVDNLFELKEFFEAKKIHIPILISLMAKDKEYIRTSYEELKEAVNEKFEIKYSDIFEKIYDVNKVFVKDSLNDDWGQVLKEVNSLLYKIISNRGIPHIAIMGPASFSMALGIALGVQNPLVVYHKAGIEYKPVIDLTDNVRKVKNIVSDYKFVKYSVENENGKNCAFILYFASHNPYDAVKNFLSKNNIDSKLVLIEPNFGKGNLPAEDWSEIVSEIMSVTQNIQFKHSCENVFFFMSCPVPLAFGVGLSFGDFAKGSIFHFDKNTGDYIEVFKIEKIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFG7</accession>
    <name>B7IFG7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Guanylate kinase</fullName>
            <ecNumber evidence="2 9">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gmk</name>
        <name evidence="11" type="ordered locus">THA_331</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for recycling GMP and indirectly, cGMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + GMP = ADP + GDP</text>
            <dbReference type="Rhea" id="RHEA:20780"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the guanylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2 9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579504.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_331"/>
    <dbReference type="EnsemblBacteria" id="ACJ74831">
        <property type="protein sequence ID" value="ACJ74831"/>
        <property type="gene ID" value="THA_331"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_331"/>
    <dbReference type="eggNOG" id="COG0194">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001715_1_2_0"/>
    <dbReference type="OMA" id="EWAVVHG"/>
    <dbReference type="OrthoDB" id="1502854at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:guanylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00328">
        <property type="entry name" value="Guanylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="10">
        <location>
            <begin position="3"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ74831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="210" mass="23906" checksum="1B022E10DE61A4F2" modified="2009-02-10" version="1">MKGTLFVVSGPSGVGKTSIISALMNKLENIVFSVSCTTRPPRPGEVDGVDYFFVSRDKFIEMREKGEFLEWAEVHGNLYGTPKKFVLENIEKGNRIILDIDVQGALQVKRNFDDAVFIFVAPPSYEVLKERLLKRGTENSQSMLKRLENAKWEMSKIVEFDYLIVNSDLEKSILAMKSIVIAESYRTRRILDEELIKRLFKGVSESETGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFJ2</accession>
    <name>B7IFJ2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579522.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_362"/>
    <dbReference type="EnsemblBacteria" id="ACJ74856">
        <property type="protein sequence ID" value="ACJ74856"/>
        <property type="gene ID" value="THA_362"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_362"/>
    <dbReference type="eggNOG" id="COG1075">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_362035_0_0_0"/>
    <dbReference type="OMA" id="RGENYNN"/>
    <dbReference type="OrthoDB" id="979618at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="791" mass="90298" checksum="332217C890178DBB" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFL5</accession>
    <name>B7IFL5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_386</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579540.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_386"/>
    <dbReference type="EnsemblBacteria" id="ACJ74879">
        <property type="protein sequence ID" value="ACJ74879"/>
        <property type="gene ID" value="THA_386"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_386"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_0"/>
    <dbReference type="OMA" id="ICGFDVQ"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="285" mass="32904" checksum="F1F0950505015AEB" modified="2009-02-10" version="1">MVVKVENLKKLYGKFEALKGISFEVEEGEVFSLLGPNGAGKTTTVEILIGLREKSEGKIYYFNKEMEKIDKKIKSVIGVSLQKTELFRELTVIETLKLFRSFYKDGYDPYEVLEKIELVEKEKERVKNLSGGQFQRLVVGLSFINDPKIIFLDEPTTGLDPQSRRKIWDIILEFKDKRKTIFLTTHYMEEAQFLSDRVCIIDNGEIIALDTPQKLIENSNLETIIEIDGVYEKFGKIIGNRTLVETNDPNSLIQKLIENKIENFTVRHPTLEDVFLKLTGKELRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFP4</accession>
    <name>B7IFP4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="6">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="6">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rdgB</name>
        <name evidence="8" type="ordered locus">THA_415</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + ITP = diphosphate + H(+) + IMP</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + XTP = diphosphate + H(+) + XMP</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579562.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_415"/>
    <dbReference type="EnsemblBacteria" id="ACJ74908">
        <property type="protein sequence ID" value="ACJ74908"/>
        <property type="gene ID" value="THA_415"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_415"/>
    <dbReference type="eggNOG" id="COG0127">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082080_0_2_0"/>
    <dbReference type="OMA" id="DCFADDT"/>
    <dbReference type="OrthoDB" id="1824064at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009146">
        <property type="term" value="P:purine nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01405">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020922">
        <property type="entry name" value="dITP/XTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="Ham1p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="PTHR11067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00042">
        <property type="entry name" value="TIGR00042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="6"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="174"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="192" mass="21686" checksum="1CF35CFC04483C75" modified="2009-02-10" version="1">MIYVATTNEHKVHEIMDILSDFNLELLKSPKKVDVEEDGKSFFENSVKKAYYYGMELNNPVISDDSGLVINALGGMPGVESARFMEGYSYEEKMKELLRRLQNFNDKSASFVCVATYFNPNSGVLISAQGVVSGTISENIRGEFGFGYDPFFIPEGYDKTFGELGESVKRQISHRSRAFRKLFELLKKVGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFT4</accession>
    <name>B7IFT4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104229.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_457"/>
    <dbReference type="EnsemblBacteria" id="ACJ74948">
        <property type="protein sequence ID" value="ACJ74948"/>
        <property type="gene ID" value="THA_457"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_457"/>
    <dbReference type="HOGENOM" id="CLU_1554570_0_0_0"/>
    <dbReference type="OMA" id="MVINIVI"/>
    <dbReference type="OrthoDB" id="2003149at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="175" mass="20792" checksum="7EA630CD974E515F" modified="2009-02-10" version="1">MKIAFVAFLITLINLALILTIAFVYQKNMEIVEIQSPPYFYFTNPYSKFFSNLKKQLILQKILSETKIYGKSPFLVESIQKLDEVKYFLKVFNVDYFEKTISKKYWWGTSEIEVLRFKDIIFYYIPIVEVAKSDEKFGKILFYSLKGKKMFLDPEDLNPISIELLKKYGVKIHEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFV2</accession>
    <name>GLGC_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucose-1-phosphate adenylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ADP-glucose pyrophosphorylase</fullName>
            <shortName evidence="1">ADPGlc PPase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ADP-glucose synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glgC</name>
        <name type="ordered locus">THA_475</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of ADP-glucose, a building block required for the elongation reactions to produce glycogen. Catalyzes the reaction between ATP and alpha-D-glucose 1-phosphate (G1P) to produce pyrophosphate and ADP-Glc.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 1-phosphate + ATP + H(+) = ADP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12120"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.7.7.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.27" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579604.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFV2"/>
    <dbReference type="SMR" id="B7IFV2"/>
    <dbReference type="STRING" id="484019.THA_475"/>
    <dbReference type="EnsemblBacteria" id="ACJ74966">
        <property type="protein sequence ID" value="ACJ74966"/>
        <property type="gene ID" value="THA_475"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_475"/>
    <dbReference type="eggNOG" id="COG0448">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_14_0_0"/>
    <dbReference type="OMA" id="QEWDVDE"/>
    <dbReference type="OrthoDB" id="1557977at2"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008878">
        <property type="term" value="F:glucose-1-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00624">
        <property type="entry name" value="GlgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011831">
        <property type="entry name" value="ADP-Glc_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005836">
        <property type="entry name" value="ADP_Glu_pyroP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023049">
        <property type="entry name" value="GlgC_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523">
        <property type="entry name" value="PTHR43523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02091">
        <property type="entry name" value="glgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00808">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00809">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword id="KW-0321">Glycogen metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000130509" description="Glucose-1-phosphate adenylyltransferase">
        <location>
            <begin position="1"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>alpha-D-glucose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>alpha-D-glucose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="178"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>alpha-D-glucose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>alpha-D-glucose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00624"/>
        </source>
    </evidence>
    <sequence length="412" mass="46146" checksum="D8BCB44B4818A3B6" modified="2009-02-10" version="1">MKNVVALILAGGQGTRLGVLTEKIAKPAVQFGGKYRLIDFTLSNCVNSGIYKIGVLTQYKPHLLNQHIGIGKPWDLDRKDGGVTILQPYYTEKKGVWYNGTADAVYRNIEFVDDYNPEYVVILSGDHIYSMDYNELLDYHKAKSALATVACMEVPLSEASRFGIMVTDLENRIIEFQEKPKQPKSTLASLGIYVFQWSFIREVLIEDAKNDQSSHDFGKDIIPKIIQTERVFAFPFDGYWKDVGTIYSYWESNLELTRPIPPFNIHDENWRIYTHSKEMPPAYIADSTKVKNSLISEGCEIYGAVSNSVLAQGVEIGKGSIVKNSVIMSNVRIGENCYIENAIIAENVVIGDFVKIGVGEFAESKLNKKVYNSEITVIGMDSIIESKVEIGKNCVVGIDMIVNKNLNSGEYI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFW4</accession>
    <name>SYS_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Seryl-tRNA synthetase</fullName>
            <shortName evidence="1">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">serS</name>
        <name type="ordered locus">THA_487</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L-seryl-tRNA(Ser)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L-seryl-tRNA(Sec)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579613.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFW4"/>
    <dbReference type="SMR" id="B7IFW4"/>
    <dbReference type="STRING" id="484019.THA_487"/>
    <dbReference type="PRIDE" id="B7IFW4"/>
    <dbReference type="EnsemblBacteria" id="ACJ74978">
        <property type="protein sequence ID" value="ACJ74978"/>
        <property type="gene ID" value="THA_487"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_487"/>
    <dbReference type="eggNOG" id="COG0172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_1_1_0"/>
    <dbReference type="OMA" id="SPCFRRE"/>
    <dbReference type="OrthoDB" id="353391at2"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00176">
        <property type="entry name" value="Ser_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43697">
        <property type="entry name" value="PTHR43697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000199513" description="Serine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="233"/>
            <end position="235"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="264"/>
            <end position="266"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="351"/>
            <end position="354"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00176"/>
        </source>
    </evidence>
    <sequence length="426" mass="49400" checksum="02F6404FBD93EECB" modified="2009-02-10" version="1">MIDVKLLRKNPEIFYDAIKKRNMDTEIIDKILEVDKEWRELVAKVNELKAKRNEFSKLVAKAKVEKDNEKASKLIEESKKIGEEIKKIEEQEKKLEEEMQNLALNIPNIPAEDVPFGKDESENIEIRRWGEPRKFDFEPKAHWDLGPELSMMDFERGAKLSGSRFTVLYSYLARLERALIQFMLDVHTKEHGYTEVWVPQLVKRDAMLWTGKLPKFEEDAYCIEKDDMFLIPTAEVPLVALHAQEILSEKDLPIKYTAYSACYRREAGSYGKDVRGMIRQHQFDKVELVWITTPERSFEDLERLTQDAERILQLLELPYRVVSLCSGDLGFVSAKTYDIEVWLPSYNSYKEISSCSNTTDFQTRRSNIRYRGSDNKLHYAHALNGSGLAVGRTLVAIVENYQNEDGSITVPKVLVPYMGVEKIEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFX7</accession>
    <name>B7IFX7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Respons regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_502</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579617.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_502"/>
    <dbReference type="EnsemblBacteria" id="ACJ74991">
        <property type="protein sequence ID" value="ACJ74991"/>
        <property type="gene ID" value="THA_502"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_502"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_628413_0_0_0"/>
    <dbReference type="OMA" id="MNGVHLC"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="306"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="436" mass="50952" checksum="1785C5F9E7470D36" modified="2009-02-10" version="1">MEVASDGLEGINKFFEFMPEIVITDYIMPNMNGVHLTRFIRSYPQFKNVGIAILTAENESINPFWAKESGANLFLQKNLDREEIIERLKKFCERDYTIEWSIEFYKIRKEPYGELVDVLEERLRDEIITSKIFSMLQYIEDEEYLLNNFFNMSINFFEPRSIHVLLITLSEGRIYSYSRDNEKFSLEHVKEKLFEDLKRPVTPSNWKYVGNFSKDGRRLNNVKSFVLVHNGIEQGVILFEDVERENDFKKNLNVALEPLGLISKILNVFSDFKIKVEFDSLTKVYNKQFIISKLNELLKMHRRQKLPLSIAMIDIDDFKKINDTYGHIKGDEVLKQFSKLIKEGLRESDYLGRYGGEEFLVIFPATSCFDAKKVIERILDKVNMYNWKEELGIEKVTFSAGVCCDLSKTAHGLINMADEKLYRAKKNGKNQVVGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFY3</accession>
    <name>B7IFY3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dihydroorotase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_508</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579622.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_508"/>
    <dbReference type="EnsemblBacteria" id="ACJ74997">
        <property type="protein sequence ID" value="ACJ74997"/>
        <property type="gene ID" value="THA_508"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_508"/>
    <dbReference type="eggNOG" id="COG0044">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_1_2_0"/>
    <dbReference type="OMA" id="NIDILCY"/>
    <dbReference type="OrthoDB" id="1319925at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="378" mass="44038" checksum="7A7E69B84EF7EA76" modified="2009-02-10" version="1">MKYISVKVYDPYQKKLFYEEISLPKNIHYENLVVVPTFFDFHTHVRLLEDQEDYESLKKACIAGGFSEVLIQPNTKPRLENSNVHEKHKKLSENPYVKFYRTTSFFGFQEPNNIDILCYSTDGIEYNYNELVENFSKKKPHLLLDHSQMFEHPGIFYKKIENLPKRPVTNEAVAVARTILTGIEFGFKDFHIQHISSIYTLEMIKFLKKYANITCEVTPHHLLLTNEHIKNSNFKINPPLADQQTREFLIEAVKNNDIDILATDHAPHPDKKNDFEKAPYGTSNIEIAFSAFYTAIEDLMIVINKLCDTPRRRLKIKPKFDSENFVVVDLNQEFIVDSKKFYSKGKNCAFDGMKLKGKVVGVKINGKWGFWNGEYLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFY8</accession>
    <name>B7IFY8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sugar ABC transporter, periplasmic sugar-binding protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_513</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579626.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_513"/>
    <dbReference type="EnsemblBacteria" id="ACJ75002">
        <property type="protein sequence ID" value="ACJ75002"/>
        <property type="gene ID" value="THA_513"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_513"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_3_1_0"/>
    <dbReference type="OMA" id="WSRFTGY"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="419" mass="47341" checksum="ECBF3AAADD969A67" modified="2009-02-10" version="1">MKKLLVILLSLVVIFSFAKTKVVFWHAMGGNHGKTLEQIVNSFNESHPDIEVEAIYVGNYGALSQKLLAGAQAGELPTIAQAYSNWTAKLIQSGVVQKLNDFLNDPKIGLAKEEWEDVYEPLRKNCMWGNDIYAVPFNKSLYILYYNADLLTLYGVDVPKSINELYYAAKVLTEDVDGDGKPDQYGFGFRTTADLFQILLSLRGGSILRMENGKWVSNIDSQETRDVLSFVRKIVDEGIAYYQGGYMNDPFGQQKVVMYIDTIAGRKYVESSVKGKFNWGWAPVPVWKTRNVPFAGTDIIMFANATDEEKKAAWEFMKYLISPDVTAFWSVNTGYIPVRKSALETNIWKENVKSDPLAEIPLTQIDNAIFDPQIGVWYEIRNVVGNMFSDFVNGKVDMETAIKTADDQIKKYLAEEYGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFZ1</accession>
    <name>B7IFZ1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Primosomal protein N'</fullName>
            <ecNumber evidence="6">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-dependent helicase PriA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">priA</name>
        <name evidence="9" type="ordered locus">THA_516</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the primosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the helicase family. PriA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_516"/>
    <dbReference type="EnsemblBacteria" id="ACJ75005">
        <property type="protein sequence ID" value="ACJ75005"/>
        <property type="gene ID" value="THA_516"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_516"/>
    <dbReference type="eggNOG" id="COG1198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013353_4_1_0"/>
    <dbReference type="OMA" id="RCHYCGY"/>
    <dbReference type="OrthoDB" id="1132322at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00983">
        <property type="entry name" value="PriA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005259">
        <property type="entry name" value="PriA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042115">
        <property type="entry name" value="PriA_3primeBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041236">
        <property type="entry name" value="PriA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040498">
        <property type="entry name" value="PriA_CRR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18074">
        <property type="entry name" value="PriA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18319">
        <property type="entry name" value="PriA_CRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00595">
        <property type="entry name" value="priA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 6" id="KW-0639">Primosome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="220"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="461"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="6">
        <location>
            <begin position="437"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="6">
        <location>
            <begin position="464"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="723" mass="83502" checksum="1DC345839C7E3978" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFZ9</accession>
    <name>B7IFZ9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">L-serine dehydratase</fullName>
            <ecNumber evidence="1">4.3.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sdaA1</name>
        <name evidence="3" type="ordered locus">THA_524</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-serine = NH4(+) + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:19169"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="EC" id="4.3.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur dependent L-serine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.17" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100648.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_524"/>
    <dbReference type="EnsemblBacteria" id="ACJ75013">
        <property type="protein sequence ID" value="ACJ75013"/>
        <property type="gene ID" value="THA_524"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_524"/>
    <dbReference type="eggNOG" id="COG1760">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022305_2_0_0"/>
    <dbReference type="OMA" id="FVEVPCV"/>
    <dbReference type="OrthoDB" id="225730at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005130">
        <property type="entry name" value="Ser_deHydtase-like_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004642">
        <property type="entry name" value="Ser_deHydtase_asu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03313">
        <property type="entry name" value="SDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00718">
        <property type="entry name" value="sda_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="1" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SDH_alpha" evidence="2">
        <location>
            <begin position="11"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="285" mass="30106" checksum="C4EC9CEAD81A8531" modified="2009-02-10" version="1">MTWKEISEMSKNMELHEVILTLEMLENGDDPVNLKNMLGDILKVILEESEKQYGKKQKTLTGLTGENAFKFRNYSPKLMGEFNYIATTTALSIAESNASMGRIAACPTAGSSGIIPGVYYSLYKLFNPSFEELLNSFIVSGGVGNVIAKKATLSGAAGGCQAEIGAAAAMAAAGLSYFFIKSADVAGNAAALALKSLMGLVCDPVGGFVEVPCVKRNGNIVNLAISSAELAMSGIKSVIPLDEVIDAMYKVGKSLPESLRETGEGGIAATPTARKLIDNIKNKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG19</accession>
    <name>B7IG19_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579641.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_545"/>
    <dbReference type="EnsemblBacteria" id="ACJ75033">
        <property type="protein sequence ID" value="ACJ75033"/>
        <property type="gene ID" value="THA_545"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_545"/>
    <dbReference type="HOGENOM" id="CLU_653699_0_0_0"/>
    <dbReference type="OMA" id="QFTIREP"/>
    <dbReference type="OrthoDB" id="1064694at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="420" mass="47071" checksum="D92D3C1EEB2D886C" modified="2009-02-10" version="1">MKKIIVIFLVLFSLTLFSFNIPRYIGSNDVEIEFENLDGYFNGVAIKDKKINGLDFEEGVHSLRLVGSYQEFLFKVIIDTIPPTSTIFTTRDPNLVILENEVLKINYENRINFFGEKITGSFKRDEKAPFLFCNIDEAKNMGGFSIVPPSISNITPLDSKTPISGINNKNILLSSKSPYKIVGRVVIPEKAVLFFEPGVELKSIGKSGITVKGTVYIPENVSFSGYISFDVSENGKFVSKTKNFDGEISSNGGQLVYIENAHLENVNVSKTNVVVIKNSVISSINAKHIAFLVLENSTITNLNIANTREVILNNIDSTNSRVEGLTNINIYNLRSKSLTVADFSNIVIRFSQIEKVTFERGVYFHGKNVEFASLKLNTFCVGKFYKSKIEKVETLKSKLLKRLVEIENIVSDKKSTIEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG30</accession>
    <name>B7IG30_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_556</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579646.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_556"/>
    <dbReference type="EnsemblBacteria" id="ACJ75044">
        <property type="protein sequence ID" value="ACJ75044"/>
        <property type="gene ID" value="THA_556"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_556"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_0"/>
    <dbReference type="OMA" id="QSDFEER"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="143"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="204"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="398" mass="45832" checksum="CAF0911BDED1A1C5" modified="2009-02-10" version="1">MSISSKVTYITTVITAITMAIVLILLYSSIRLMIYQSTKKDLIESVRPWLVTPMGRMHNPKNDIYISKDGIVIVDPYEIGPINKIGKFEINGRTYIFLRAENLIVGKDITPIVNYLNNIKKIFVYIFAISLSLISFLTYFITKKSTKHLRNFVSELSKIKGDNLSYRFIKPNTHDEVDELVEKFNELMDRVEKNYKLQEEFVSNVSHELKTPIANLIGYSKMLERWGHKDEQILKEAIDSIAQTSKKMKDLVENMILLSKNLELDMEQINLRTLVEGIVAGYKDIEIKIIGDGTILANKEALEIVIKNLVDNAIYHGKAPIEIKLYQDRIEVSDHGEGISNDLKERIFEKFHKSRKSKGHGLGLYIVKKLCDQMGLNIYIKNDKYTTFVVERSEKDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG31</accession>
    <name>B7IG31_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Chaperonin HslO</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hslO</name>
        <name evidence="6" type="ordered locus">THA_557</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579647.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_557"/>
    <dbReference type="EnsemblBacteria" id="ACJ75045">
        <property type="protein sequence ID" value="ACJ75045"/>
        <property type="gene ID" value="THA_557"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_557"/>
    <dbReference type="eggNOG" id="COG1281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054493_1_0_0"/>
    <dbReference type="OMA" id="DMQCECC"/>
    <dbReference type="OrthoDB" id="1406579at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00498">
        <property type="entry name" value="Hsp33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000397">
        <property type="entry name" value="Heat_shock_Hsp33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016154">
        <property type="entry name" value="Heat_shock_Hsp33_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016153">
        <property type="entry name" value="Heat_shock_Hsp33_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30111">
        <property type="entry name" value="PTHR30111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01430">
        <property type="entry name" value="HSP33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005261">
        <property type="entry name" value="Heat_shock_Hsp33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118352">
        <property type="entry name" value="SSF118352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64397">
        <property type="entry name" value="SSF64397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="287" mass="32050" checksum="135CF0327D4210FF" modified="2009-02-10" version="1">MLKNGIAYKALVRFSVIDSTQIVRAATEKHGLSPISAVALGRLLTGAALMIPWLSEKETLTYIIEGSNQIKYIASQAKSDGTVRGYVIPKIVETMTNELGKFDLKNAIGKGTLKVVRDLNLKTPYVTPVELVSGEIAEDLAHYFAVSEQLPTAIALGVLVDKNGIKKAGGIVIQILDKNLPESDILEIEKKFKEVTPISNFLENNTVEDAVKHIFGDKIEKIEEREVEFKCNCSYQKAVESLKLLKVDELKEMLNEGKAEVECKWCSTKYYIEKEDIEKILEEKEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG33</accession>
    <name>B7IG33_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Single-stranded-DNA-specific exonuclease RecJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_559</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579648.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_559"/>
    <dbReference type="EnsemblBacteria" id="ACJ75047">
        <property type="protein sequence ID" value="ACJ75047"/>
        <property type="gene ID" value="THA_559"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_559"/>
    <dbReference type="eggNOG" id="COG0608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009736_3_0_0"/>
    <dbReference type="OMA" id="YIPHRIE"/>
    <dbReference type="OrthoDB" id="491634at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004610">
        <property type="entry name" value="RecJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041122">
        <property type="entry name" value="RecJ_OB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17768">
        <property type="entry name" value="RecJ_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00644">
        <property type="entry name" value="recJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="6">
        <location>
            <begin position="78"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="7">
        <location>
            <begin position="315"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="RecJ_OB" evidence="8">
        <location>
            <begin position="449"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="975" mass="112642" checksum="38325CEFB8B116F6" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG38</accession>
    <name>B7IG38_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">ATP synthase subunit a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP synthase F0 sector subunit a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">F-ATPase subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">atpB</name>
        <name evidence="13" type="ordered locus">THA_565</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell inner membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the ATPase A chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100572.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_565"/>
    <dbReference type="EnsemblBacteria" id="ACJ75052">
        <property type="protein sequence ID" value="ACJ75052"/>
        <property type="gene ID" value="THA_565"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_565"/>
    <dbReference type="eggNOG" id="COG0356">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041018_2_3_0"/>
    <dbReference type="OMA" id="FTHAVRL"/>
    <dbReference type="OrthoDB" id="867266at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01393">
        <property type="entry name" value="ATP_synth_a_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045082">
        <property type="entry name" value="ATP_syn_F0_a_bact/chloroplast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000568">
        <property type="entry name" value="ATP_synth_F0_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023011">
        <property type="entry name" value="ATP_synth_F0_asu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035908">
        <property type="entry name" value="F0_ATP_A_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42823">
        <property type="entry name" value="PTHR42823"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00119">
        <property type="entry name" value="ATP-synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00123">
        <property type="entry name" value="ATPASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81336">
        <property type="entry name" value="SSF81336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01131">
        <property type="entry name" value="ATP_synt_6_or_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00449">
        <property type="entry name" value="ATPASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 11 12" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 11 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 11 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ75052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="280" mass="31269" checksum="23702B3D0BFEBE03" modified="2009-02-10" version="1">MKKKISTGLLIFLIIYTVVGIINAIYFVPKDTAEVTKGLANRWIVQFSDTKAWYTRINPLTLIMSWAIIIGLIIFAVRVGKNFKVIPDRKQALSESVMDFMYEVVESVIDDPKFVKPTFMIATTLFIYISIANVIGGAIPGINVSVVDGHVKFTLFQDTWFSPTADLNTNLTYALMVFVISHAFAIKAKGFGGWLKSWMEPTPIMLPMNIIGELAKPISHSLRLFGNIGGGAILTFMLSYLVKYLFMPVVFWGYFGMFVGLVQALVFSMLAVAYISEKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG69</accession>
    <name>B7IG69_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_596</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579670.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75083">
        <property type="protein sequence ID" value="ACJ75083"/>
        <property type="gene ID" value="THA_596"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_596"/>
    <dbReference type="eggNOG" id="COG1672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_733493_0_0_0"/>
    <dbReference type="OMA" id="RWNESGN"/>
    <dbReference type="OrthoDB" id="914470at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_2" evidence="1">
        <location>
            <begin position="25"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="387" mass="45077" checksum="AF1AC6977A28A46D" modified="2009-02-10" version="1">MENKKLKYIVEALFDKPIDDKELLVNRKKELEHLNKIAYFQPMGIFGVCGETGVGKTTTLNFINAENAKRLYILITEKDSKEVIIGDFLYKLSLLQINSKNKKVRKLATETRDFILNEMSYNTSYGGGVNFAANVSYEKSINNKKRFNIYSVKEYIDNLLSLLIEEYGKVLIIVDELDKEKKEDVLNILDSLKSVLFKENIITIVSLPFSIYREYSNDRMRWNESGNLENIFKDMVFLEPLTAEDIKKMILKRIKEYPDYFEMDALDEIAAFSDGNPRDALWIAQQVALFNTDKNKITKELVKKGIRRIISQNLNNSLSFTDIQKTILKELAKEPVDRTTLVKRLEKKVKRQTVYTYINRMKDEGIILEKSGILYLPSKIKYYIEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG73</accession>
    <name>B7IG73_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_600"/>
    <dbReference type="EnsemblBacteria" id="ACJ75087">
        <property type="protein sequence ID" value="ACJ75087"/>
        <property type="gene ID" value="THA_600"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_600"/>
    <dbReference type="eggNOG" id="ENOG5032FKI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106275_1_0_0"/>
    <dbReference type="OMA" id="DFIDLYC"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014942">
        <property type="entry name" value="AbiEii"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08843">
        <property type="entry name" value="AbiEii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="191" mass="23004" checksum="545F0AC1101ED0B8" modified="2009-02-10" version="1">MRLEIFHDFYLAGGTCLLLKYNRRPPSLDFDFFLFPDKDFSNVYENTIYSKLNVAFAYKDPQTLIFELDDIKISLCKYKYPLLKQPDKVGKLYLASDEDIACMKMSAIAQRGLKKDFYDLWFLINIHKWTIKELVSMLETKYKGYNPLIFLKALVYFEDAEKNKDFREVEEKWEDIKKFFNDFVRSFREYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG90</accession>
    <name>B7IG90_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-2-macroglobulin family N-region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_618</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. Bacterial alpha-2-macroglobulin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579686.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_618"/>
    <dbReference type="EnsemblBacteria" id="ACJ75104">
        <property type="protein sequence ID" value="ACJ75104"/>
        <property type="gene ID" value="THA_618"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_618"/>
    <dbReference type="eggNOG" id="COG2373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_249027_0_0_0"/>
    <dbReference type="OMA" id="EYFPDTA"/>
    <dbReference type="OrthoDB" id="231654at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041246">
        <property type="entry name" value="Bact_MG10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17973">
        <property type="entry name" value="bMG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="SSF48239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="A2M_N_2" evidence="2">
        <location>
            <begin position="465"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="A2M" evidence="3">
        <location>
            <begin position="653"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="1304" mass="151321" checksum="923C1070F2BCFF06" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGB8</accession>
    <name>B7IGB8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_655</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_655"/>
    <dbReference type="EnsemblBacteria" id="ACJ75132">
        <property type="protein sequence ID" value="ACJ75132"/>
        <property type="gene ID" value="THA_655"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_655"/>
    <dbReference type="eggNOG" id="COG4586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_0"/>
    <dbReference type="OMA" id="LIWDIPP"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="52"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="332" mass="38299" checksum="E427428D46F27884" modified="2009-02-10" version="1">MIKVEKLTRNYKIPNNKGNFFVRLFNSRYKTITALNNVSFTISKGEKVAVLGLNGSGKSTLFKILTGIISPSSGSCKCLDYDPFNERKKYVKNIGVLFGQKTLLIPELSVYDCLKLYKAVYELKQKDIDYRLKIFDKHFGVEKLLHRIVRTLSLGERMKAEILMATIHDPLLFFFDEPTIGLDVQAKNAFKDFLLNYPFGSEKTVLITTHDISVIKEFATRILLLNNGKLVLDIKTKYLKDKFGFKKLVIDFKREPTKSIMENIKTFGCPIEKNKNKLIIKVPVNEKEKIETIKKKIVTDESIISFKVENMDYEETINWLMGEFYADVQKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGD3</accession>
    <name>B7IGD3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-ribose-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579716.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_672"/>
    <dbReference type="EnsemblBacteria" id="ACJ75147">
        <property type="protein sequence ID" value="ACJ75147"/>
        <property type="gene ID" value="THA_672"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_672"/>
    <dbReference type="eggNOG" id="COG1879">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_3_2_0"/>
    <dbReference type="OMA" id="QAVFAHN"/>
    <dbReference type="OrthoDB" id="1378040at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025997">
        <property type="entry name" value="SBP_2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13407">
        <property type="entry name" value="Peripla_BP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peripla_BP_4" evidence="1">
        <location>
            <begin position="23"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="289" mass="31005" checksum="17D985FDE74E6F64" modified="2009-02-10" version="1">MKRYIFVILILVVFAFTAFSYKIGLSLSTLNNPFFVTLKDGAVAKAKELGINLIVVDAQDKPSKQLNDIEDLIQQKVDMIIVNPTDSDAIVAAVEEANEAGIPVITVDRGSNGGKVVAHIASDNILGGAMAARFIAEKLNGNGKVIELVGIPGTSAARDRGKGFETELKKYPGLKLIAKQTANFNRAEGLTVMENLLEAYPDIDAVFAQNDEMALGAIEAIKAAGKLGKIIVVGFDAIPDAIEAVKKGEMAATIAQQPYLMGQLAIQKAYDYLESHTIYIPVELKLVTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGE1</accession>
    <name>B7IGE1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Iron-molybdenum cofactor-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_683</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579724.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_683"/>
    <dbReference type="EnsemblBacteria" id="ACJ75155">
        <property type="protein sequence ID" value="ACJ75155"/>
        <property type="gene ID" value="THA_683"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_683"/>
    <dbReference type="eggNOG" id="COG1433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104194_2_2_0"/>
    <dbReference type="OMA" id="ISPHFGG"/>
    <dbReference type="OrthoDB" id="1995095at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00851">
        <property type="entry name" value="MTH1175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033913">
        <property type="entry name" value="MTH1175_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="SSF53146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitro_FeMo-Co" evidence="1">
        <location>
            <begin position="14"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="118" mass="13361" checksum="B8ED3E2EEA820E5B" modified="2009-02-10" version="1">MIIAIPVIESNGENSQISEHFGHSPFFAFYDTEKEKLRIENNSLEEHGPGDVPNFLYSKGVNVIIVRGIGQRAISFFEQLGIKVIRGASGSVKDIINAFLENKIEDKEYQVKEKFHNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGH0</accession>
    <name>B7IGH0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_713</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579748.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_713"/>
    <dbReference type="EnsemblBacteria" id="ACJ75184">
        <property type="protein sequence ID" value="ACJ75184"/>
        <property type="gene ID" value="THA_713"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_713"/>
    <dbReference type="eggNOG" id="ENOG50330IY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170247_1_0_0"/>
    <dbReference type="OMA" id="LTEYGCY"/>
    <dbReference type="OrthoDB" id="1946325at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="89" mass="10194" checksum="9DB1CA6ED250831C" modified="2009-02-10" version="1">MTDITVRTVMAILIDNREKAATKVQELLTEYGCYIKTRLGLHEGVPQYCSNSGLIILELVDDESKHEELKEKLNQIPGVKCDLMRLRFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGJ1</accession>
    <name>B7IGJ1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="2">6.3.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">purD</name>
        <name evidence="9" type="ordered locus">THA_739</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579765.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_739"/>
    <dbReference type="EnsemblBacteria" id="ACJ75205">
        <property type="protein sequence ID" value="ACJ75205"/>
        <property type="gene ID" value="THA_739"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_739"/>
    <dbReference type="eggNOG" id="COG0151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027420_3_0_0"/>
    <dbReference type="OMA" id="KATVCKY"/>
    <dbReference type="OrthoDB" id="932854at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PTHR43472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="8">
        <location>
            <begin position="88"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="397" mass="44295" checksum="20F97D81C294B39B" modified="2009-02-10" version="1">MKVCIVGSGGREHAMGYAFEKAGFEVFYTPGNGLTENNIDFESLKYFNGLIIPGSEEYLANGIAEKYANVFGPDSRASRLESSKIYAKQFMSIYNLTTPMFEIASNREELLEKIEKFYPPYVLKADGLAKGKGVLIVENKDEAVELGSKLISGELIAGVSGPIVIDEFLPGRELSAMAVVNGDNFSLFPFIQDYKKINTGNTGPNTGGMGSFGPIEIDGELKTKIEELFAKTLYGLKKEGVFYRGFLYLGLMIYEKTPYILEYNVRLGDPETEVIVATNPDNFVECVLKAYNKENIPEFNPKYYALDVVLASKGYPGKYEKGKEIRNLFPLQGEDFVIFGAGVKKENDKYYTNGGRVLHCVAFSENKEEAKKRAYEISEKIDFDGKIFRKDIGVIYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGK2</accession>
    <name>B7IGK2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75216">
        <property type="protein sequence ID" value="ACJ75216"/>
        <property type="gene ID" value="THA_750"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_750"/>
    <dbReference type="HOGENOM" id="CLU_3085725_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="52" mass="6349" checksum="BA3B30B28F64A1D6" modified="2009-02-10" version="1">MLEDIEGSILLADRGYWQWEFAEKMKEKMKLYVRPSGRKGKEFMKREINRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGM1</accession>
    <name>B7IGM1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type dipeptide/oligopeptide/nickel transport system, ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_772</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100326.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_772"/>
    <dbReference type="EnsemblBacteria" id="ACJ75235">
        <property type="protein sequence ID" value="ACJ75235"/>
        <property type="gene ID" value="THA_772"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_772"/>
    <dbReference type="eggNOG" id="COG0444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_0"/>
    <dbReference type="OMA" id="CRFKDRC"/>
    <dbReference type="OrthoDB" id="1101128at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="318" mass="35806" checksum="F27E72919B3DDCB8" modified="2009-02-10" version="1">MLAVNGLKLYYKTLKGYVKAVDDVSFDLKDNEILGLAGESGCGKSTLVNGLILLKPPLNYFGGNVILDNELLPINDFEKMNDFRYKRLSIIPQYAMDALNPTRKIGVYIKEVLKSKGINYDSIHDRLIERLKFVNLPERVLKMYPIELSGGMKQRLVMVISTLLNPSLLIADEVTSALDVSSQKSVCLMIKGFKDEGIVKSLIFVTHDLSVLYQIADRIMVMYAGKVAEIGTTQQIIENPVHPYTKMLLSSLPEVGIRYTDTRLKGIPGRPPQLLNPPKGCRFKDRCPFYSSQCDSEPPKVQVEDNHIAYCWKVNKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGN0</accession>
    <name>B7IGN0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fe-hydrogenase, subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_782</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100349.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_782"/>
    <dbReference type="EnsemblBacteria" id="ACJ75244">
        <property type="protein sequence ID" value="ACJ75244"/>
        <property type="gene ID" value="THA_782"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_782"/>
    <dbReference type="eggNOG" id="COG1905">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177584_0_1_0"/>
    <dbReference type="OMA" id="CMGSACH"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="75" mass="8224" checksum="2B3E7534C15A74AB" modified="2009-02-10" version="1">MVIKICMGSSCHLKGSYEVVEKIKEMNLEGVKLYGSLCFGKCANGINIEIDGVLISSVTPDNVKEKIEKFLKEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGT6</accession>
    <name>B7IGT6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_838</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100456.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_838"/>
    <dbReference type="EnsemblBacteria" id="ACJ75300">
        <property type="protein sequence ID" value="ACJ75300"/>
        <property type="gene ID" value="THA_838"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_838"/>
    <dbReference type="eggNOG" id="COG1277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064090_0_0_0"/>
    <dbReference type="OMA" id="MIAPFID"/>
    <dbReference type="OrthoDB" id="1376978at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032688">
        <property type="entry name" value="ABC2_membrane_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="265" mass="30856" checksum="9BB6DFE9050746B8" modified="2009-02-10" version="1">MLILKKELRSNIKLFIIWAFVLFLFSYMIAPFIDTVMKDSEEMINFIKSLPKFMVKIFNISENSITPEGFFGMKIMMMAQIFAGVFSIILASNLFANEFENKTIEYLLVKPISRKKIYLEKLIAILIFYTLFFLIFCASVLALFKVYVSYEYSPYILFGYSLYLYVIEIFFGFLTIFLSVIFQKTMLSISLSLSSFILMYIIDILGSANEKFSFLRYLSIFKYISIGDTINNNVVYINNSIILILLGFVISLFGLKIFTRKDVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGV1</accession>
    <name>B7IGV1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_855</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579835.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_855"/>
    <dbReference type="EnsemblBacteria" id="ACJ75315">
        <property type="protein sequence ID" value="ACJ75315"/>
        <property type="gene ID" value="THA_855"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_855"/>
    <dbReference type="eggNOG" id="ENOG5033D6E">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140162_1_0_0"/>
    <dbReference type="OMA" id="YAMWVYA"/>
    <dbReference type="OrthoDB" id="1782345at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="125" mass="14538" checksum="3790C405E41374E2" modified="2009-02-10" version="1">MKNLEASINKTSFEIAEYLENENNINKMLGVLSNYGVYALWIFSKKILKDEKNKEFWNKIFNGLEELIISFGILDKNDKGLSEKSFEKLSEDLEKVLFFKDVLEKVLVYSRYHVKAMDKGKNGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGY8</accession>
    <name>B7IGY8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_893</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579867.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_893"/>
    <dbReference type="EnsemblBacteria" id="ACJ75352">
        <property type="protein sequence ID" value="ACJ75352"/>
        <property type="gene ID" value="THA_893"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_893"/>
    <dbReference type="eggNOG" id="ENOG502ZVFE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181401_5_0_0"/>
    <dbReference type="OMA" id="NAMDSFV"/>
    <dbReference type="OrthoDB" id="2034992at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021321">
        <property type="entry name" value="DUF2922"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11148">
        <property type="entry name" value="DUF2922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="70" mass="7903" checksum="3F931856E2F74F22" modified="2009-02-10" version="1">MKRLTLVFRNQEEGKAFRVNVQNPVENIDTASLQTDAQVLIDNGLVPEGYVFDEARVLESNTNVLIDILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH08</accession>
    <name>B7IH08_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Branched-chain amino acid aminotransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_913</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100791.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_913"/>
    <dbReference type="EnsemblBacteria" id="ACJ75372">
        <property type="protein sequence ID" value="ACJ75372"/>
        <property type="gene ID" value="THA_913"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_913"/>
    <dbReference type="eggNOG" id="COG0115">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020844_3_0_0"/>
    <dbReference type="OMA" id="YADEIFL"/>
    <dbReference type="OrthoDB" id="1275805at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="SSF56752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="246" mass="28012" checksum="74534A649A982782" modified="2009-02-10" version="1">MNERSLIDNILNGIVVYETVRIYDGKPLAVKEHYKRLINSLSYLGREDEVSLNDFEKEIERNISFDRVKIYAIVSDKVELYSVGENILTKRIESIKIDISNVRHADPSSIPPNFKSLSRADVFLARQNKGDNYDVILLGQKGQVCEGSFSNVFLIKDGKVITPSLESGILEGITRTFVIDMLKESGYTVEEKIVEVKELFYADEIFLTHTSMGIVPVNYLGKFSLKTEISEKLSRMFEEYLHAKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH14</accession>
    <name>B7IH14_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579877.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_919"/>
    <dbReference type="EnsemblBacteria" id="ACJ75378">
        <property type="protein sequence ID" value="ACJ75378"/>
        <property type="gene ID" value="THA_919"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_919"/>
    <dbReference type="eggNOG" id="ENOG502ZXS1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1354058_0_0_0"/>
    <dbReference type="OrthoDB" id="1847861at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="202" mass="22593" checksum="FB94373355F7F6CC" modified="2009-02-10" version="1">MKKKIIISINLILILILSGCGLFLKPSSKPSTSSNVLKIETYVEIYDEYGNRIFVDGKINGYPIVLKDGQTGITVKISEVKSLSLNTQMFDYNYNLEGGKVKINLFKKSNLSKGTVELQKQLDWSIIVYTYEVDKSKLNIWLDNLLEKSWAKNASVKSIISSDENIIASNYLISILKNSPIGTGYFKTGAKGNVRIKSVILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH42</accession>
    <name>B7IH42_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exonuclease sbcd</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579894.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_947"/>
    <dbReference type="EnsemblBacteria" id="ACJ75406">
        <property type="protein sequence ID" value="ACJ75406"/>
        <property type="gene ID" value="THA_947"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_947"/>
    <dbReference type="eggNOG" id="COG0420">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060534_0_0_0"/>
    <dbReference type="OMA" id="DWRRAYD"/>
    <dbReference type="OrthoDB" id="1831911at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="385" mass="44381" checksum="076908F54804FBF0" modified="2009-02-10" version="1">MKILHTSDWHLDRRPAGGVGLYSNERYEDYFKAAEYIVNEAVKNKVDLVIISGDLFDKNDISPETLYKAEKLLNVLKENEIKVLAIEGNHDQVSSYKESWIWYLEKKDLAIFPKSYLDGEKFKFVPIEIDGIKFFGVSYQSNLNEKALQDLSQSLDKENNIVIVHTAIVNDLSDTLLPGCVTKEVIDAFSNKVLYIAAGHFHSFYKYPSTNPFFFIPGAPEYWDLNEKEQKGYIIFDTDSRKYTFYPSKKRKLSTYRISIDNYKDFVANLDVEKGEIINLEINSASGRYPNVGWIEGELLSKGALKINVKVIFEGQVYNSNQLKNLSKEEIEKQIIKSWGNIFSKNADDTSKLISTLKKMEREEDLIDIFDEFLNKLLEGEKDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH45</accession>
    <name>B7IH45_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sodium/phosphate symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_950</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_950"/>
    <dbReference type="EnsemblBacteria" id="ACJ75409">
        <property type="protein sequence ID" value="ACJ75409"/>
        <property type="gene ID" value="THA_950"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_950"/>
    <dbReference type="eggNOG" id="COG0306">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015355_0_1_0"/>
    <dbReference type="OMA" id="RANPHKA"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PTHR11101"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="305" mass="32158" checksum="D85196B22FFCA922" modified="2009-02-10" version="1">MYILPAIFFGWSLGANDAANIFGTAVSNRIVKYRTATIISAIFIILGAMLDGAKGIETISSISSSQIGLASISVLASAITMTTMTYLGVPVSSSQAIVGSLIVTSLIEGNVKWGVLVKLFAAWIGTPIGGIIFSFLAYKIFAVPFNKIKSIYVKEKVIFYGTLIIGAYGSYSLGANNVANITGVFAKTIGVQNAALLGGVSIALGVLTYSYKVMYTVGTQIIELDYYSSAIAVLGESITVWIYALLGIPVSTSQAIVGAVIGAGYARGSRLSSKKIILKIVSAWVNTPVISGAITFLIYYIVKMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH53</accession>
    <name>B7IH53_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Methyl-accepting chemotaxis protein (MCP) signaling domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_958</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579902.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_958"/>
    <dbReference type="EnsemblBacteria" id="ACJ75417">
        <property type="protein sequence ID" value="ACJ75417"/>
        <property type="gene ID" value="THA_958"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_958"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_362005_0_0_0"/>
    <dbReference type="OMA" id="ISAQTNM"/>
    <dbReference type="OrthoDB" id="128357at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029151">
        <property type="entry name" value="Sensor-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103190">
        <property type="entry name" value="SSF103190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="477"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="520"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="765" mass="86920" checksum="5E0D3D3C8FD72ED2" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHA3</accession>
    <name>B7IHA3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Metyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1009</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1009"/>
    <dbReference type="EnsemblBacteria" id="ACJ75467">
        <property type="protein sequence ID" value="ACJ75467"/>
        <property type="gene ID" value="THA_1009"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1009"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_18_0"/>
    <dbReference type="OMA" id="KHASAQI"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="Methyl-accepting transducer" evidence="3">
        <location>
            <begin position="7"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="279" mass="30184" checksum="E8686BD263A1B3DA" modified="2009-02-10" version="1">MGGITELSTQVADTAVQISNDAERISEAVTSNVNTISQTITEQNQIINDLNTAVEKIITAAKNVENSASGIKLMSEEFEKITKESEELKNQASTIQEIANTVMNIAEQTNLLALNAAIEAARSGEAGKGFAVVADEIRKLAEESKESAVKISQFLSTVSNGISQLSLSVTKGYEELKNQSSELESSALKSKESSEIISNITSQLNTLVSTLNSETEKLENITTSIQNLLAISEESSATAEEISATIQKFLDELESVFENVRKTISLIQTIQDNFNEIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHB1</accession>
    <name>B7IHB1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1017</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579935.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1017"/>
    <dbReference type="EnsemblBacteria" id="ACJ75475">
        <property type="protein sequence ID" value="ACJ75475"/>
        <property type="gene ID" value="THA_1017"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1017"/>
    <dbReference type="eggNOG" id="COG4608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_0"/>
    <dbReference type="OMA" id="GRYPHMF"/>
    <dbReference type="OrthoDB" id="1303270at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="2"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="49"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="323" mass="36783" checksum="C0843EB2493756E2" modified="2009-02-10" version="1">MIKVENLKKYFPVKRTVGEVLLRVPQRFVKAVDGVSFSISKGETFGLVGESGSGKTTTGRLILRLIEPTSGKIFFEDTDITKLSKEELRKFRKNMQIIFQDPMAALNPYMKVGDAIKHGLEIHNIGGSNQERKMLVMKMLERVNLSPPEDFYYRYPHELSGGQRQRIVIARALILRPKFVVADEAIAMLDVSVRSQLLKLLIDLKNEFNLTFLFITHDLATTKYICNKIGVMYLGKIVEIGDFKEIYTEPLHPYTKALISAVPEPDPKRKKKKIIPEGEVPNPINPPKGCKFHPRCPYAMDICKETEPSLKKVKNHIVACHLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHF5</accession>
    <name>B7IHF5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SIS domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1063</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579970.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1063"/>
    <dbReference type="EnsemblBacteria" id="ACJ75519">
        <property type="protein sequence ID" value="ACJ75519"/>
        <property type="gene ID" value="THA_1063"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1063"/>
    <dbReference type="eggNOG" id="COG2222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_2_0_0"/>
    <dbReference type="OMA" id="WTVGLAH"/>
    <dbReference type="OrthoDB" id="1046291at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="3">
        <location>
            <begin position="21"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="302" mass="34672" checksum="4575B30A2C30B44F" modified="2009-02-10" version="1">MNYTYSEIKRIPELLKRIENYSFEFNKEKRYIFVGCGSSFNLSLTASYILEKFGIKSKVLTGGKVITFNYVPDADVGIFISRTGESTETVKAAEIFKEKGVHTIGITCEKGTTLEKVCNETFVFDFLHEESIVMTGSFVSILHFLVKRYSIVEKASKILERSEKFIENLDLKKYNHFIFLGFDEEYGISKEGALKIQEMAKQFVEYHEPLEYRHGPVSRISEHSLVVINSKDTENEYNLKKDLKKYTKVILLGRNGDIDIEYDNGLETPLKMIFSQVLSFKKAVVEGLNPDRPEKLSKSVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHG6</accession>
    <name>B7IHG6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobalamin biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cbiH</name>
        <name evidence="6" type="ordered locus">THA_1074</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1074"/>
    <dbReference type="EnsemblBacteria" id="ACJ75530">
        <property type="protein sequence ID" value="ACJ75530"/>
        <property type="gene ID" value="THA_1074"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1074"/>
    <dbReference type="eggNOG" id="COG1010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047948_2_0_0"/>
    <dbReference type="OMA" id="VWDDRMV"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11646">
        <property type="entry name" value="Precorrin_3B_C17_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006363">
        <property type="entry name" value="Cbl_synth_CobJ/CibH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="236" mass="26711" checksum="6D855C79C820FA06" modified="2009-02-10" version="1">MIKLVGIGPGDLQYLTFRAKDALEKCDVVIGYEKYVEQIAELIKSKVIYKYKMGQEEERCKRAVELSNEGKDVCLVCGGDPGIYSLSSIILNLTDNVEIIPGISALNACASILGSPISNDFVVLSFSDYNVDISEIKKRVELACKVDFVMVIYNPISKVRLEKTKEIFQVIVDNKPAKTKVALVKNCYRADFSMRIIELSQIFENIDFIDMNTTVIVGNKYTYENFGRLITPRWYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHN1</accession>
    <name>B7IHN1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rhomboid family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1143</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580031.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1143"/>
    <dbReference type="MEROPS" id="S54.027"/>
    <dbReference type="EnsemblBacteria" id="ACJ75595">
        <property type="protein sequence ID" value="ACJ75595"/>
        <property type="gene ID" value="THA_1143"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1143"/>
    <dbReference type="eggNOG" id="COG0705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055068_5_1_0"/>
    <dbReference type="OMA" id="IAYWAHA"/>
    <dbReference type="OrthoDB" id="2053476at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="SSF144091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Rhomboid" evidence="6">
        <location>
            <begin position="79"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="233" mass="27354" checksum="85127A2B034C8DD5" modified="2009-02-10" version="1">MFPLYDTIPSRKKPYITYTIIFINVVIFLYELILNNAELQQFFFNYGVVPLRYTHVLTNKIKYVYQWNLIENIIYSNPIFPFISHMFVHGGWSHILGNMWFLWIFGDNVEDRMGHFNYLLFYLTGGLFALFFHMAFNLFSPYPLVGASGAISAVMGAYFVNFWYSKIVSLVIWFVPFLIEIPAFIYLFIWFTIQILNGTLSNITGSGVAYWAHAGGFIYGMIIGSRVRRRYYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHP1</accession>
    <name>B7IHP1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FG-GAP repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580041.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1153"/>
    <dbReference type="EnsemblBacteria" id="ACJ75605">
        <property type="protein sequence ID" value="ACJ75605"/>
        <property type="gene ID" value="THA_1153"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1153"/>
    <dbReference type="eggNOG" id="ENOG5033TI8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_479750_0_0_0"/>
    <dbReference type="OMA" id="YNGDGYK"/>
    <dbReference type="OrthoDB" id="238122at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13517">
        <property type="entry name" value="FG-GAP_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="SSF69318"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="572" mass="64185" checksum="24DB6AD41BA4E4AF" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHP3</accession>
    <name>B7IHP3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prepilin-type N-cleavage/methylation domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580043.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1155"/>
    <dbReference type="EnsemblBacteria" id="ACJ75607">
        <property type="protein sequence ID" value="ACJ75607"/>
        <property type="gene ID" value="THA_1155"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1155"/>
    <dbReference type="eggNOG" id="ENOG502ZWN2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132012_0_0_0"/>
    <dbReference type="OMA" id="YRNISTE"/>
    <dbReference type="OrthoDB" id="1946424at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045584">
        <property type="entry name" value="Pilin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54523">
        <property type="entry name" value="SSF54523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00409">
        <property type="entry name" value="PROKAR_NTER_METHYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="164" mass="18776" checksum="8540C83ACC4BC571" modified="2009-02-10" version="1">MVKTHKGFTLVELLIVIVIISIVMSVSFVGFQTMYKNYSTESKLSGILQNLLFLFIDARRTAIIEDDVVCIRYANGEFSYYIDNNLDGTPDSNVISSFKTDENIKVYLNDEEKSSFLIYTYDSLFFKNNAGVFESSYSNTEIKILLDSREKILKIENSLPKVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHP9</accession>
    <name>B7IHP9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1161</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580049.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1161"/>
    <dbReference type="EnsemblBacteria" id="ACJ75613">
        <property type="protein sequence ID" value="ACJ75613"/>
        <property type="gene ID" value="THA_1161"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1161"/>
    <dbReference type="eggNOG" id="ENOG503315M">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2286677_0_0_0"/>
    <dbReference type="OMA" id="KYEHDFG"/>
    <dbReference type="OrthoDB" id="1986938at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="102" mass="11461" checksum="DB9664729B896E6D" modified="2009-02-10" version="1">MKAKKGDWVQIEKLLLKPEERTAPLPDDTKKVPLKMRVKGFLQNEEANIGDEVEILTLIGRRVTGTLVAINPKYEHDFGEPVPELLTIGIELRKILEGDSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHS0</accession>
    <name>B7IHS0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Oligoendopeptidase F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1184</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580064.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1184"/>
    <dbReference type="EnsemblBacteria" id="ACJ75634">
        <property type="protein sequence ID" value="ACJ75634"/>
        <property type="gene ID" value="THA_1184"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1184"/>
    <dbReference type="eggNOG" id="COG1164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021290_3_0_0"/>
    <dbReference type="OMA" id="YQREVYR"/>
    <dbReference type="OrthoDB" id="1935578at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013647">
        <property type="entry name" value="OligopepF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042088">
        <property type="entry name" value="OligoPept_F_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PTHR11804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08439">
        <property type="entry name" value="Peptidase_M3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M3_N" evidence="8">
        <location>
            <begin position="108"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M3" evidence="7">
        <location>
            <begin position="191"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="579" mass="67411" checksum="A4EF2EC045B74920" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHT1</accession>
    <name>B7IHT1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Flagellar biosynthetic protein FliQ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">fliQ</name>
        <name evidence="10" type="ordered locus">THA_1195</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Role in flagellar biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the FliQ/MopD/SpaQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101414.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1195"/>
    <dbReference type="EnsemblBacteria" id="ACJ75645">
        <property type="protein sequence ID" value="ACJ75645"/>
        <property type="gene ID" value="THA_1195"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1195"/>
    <dbReference type="eggNOG" id="COG1987">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164516_2_0_0"/>
    <dbReference type="OMA" id="TMPWMLN"/>
    <dbReference type="OrthoDB" id="1939288at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002191">
        <property type="entry name" value="Bac_export_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006305">
        <property type="entry name" value="FliQ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34040">
        <property type="entry name" value="PTHR34040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01313">
        <property type="entry name" value="Bac_export_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004669">
        <property type="entry name" value="FliQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00952">
        <property type="entry name" value="TYPE3IMQPROT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01402">
        <property type="entry name" value="fliQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="4 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="10" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="88" mass="9879" checksum="8709BBA7C51DFC79" modified="2009-02-10" version="1">MTIEVFLDVFGLGIKTLLTVILPPLLISLIVGLLISIFQAATQINEQTLTFAPRIIVLFLTLLFLGGWMIQQVIDLAREIITKYFSMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHZ2</accession>
    <name>B7IHZ2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR-associated endoribonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1261</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580099.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1261"/>
    <dbReference type="EnsemblBacteria" id="ACJ75706">
        <property type="protein sequence ID" value="ACJ75706"/>
        <property type="gene ID" value="THA_1261"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1261"/>
    <dbReference type="eggNOG" id="COG1583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089858_2_0_0"/>
    <dbReference type="OMA" id="FGMVKVD"/>
    <dbReference type="OrthoDB" id="908607at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045747">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36984">
        <property type="entry name" value="PTHR36984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01881">
        <property type="entry name" value="Cas_Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005054">
        <property type="entry name" value="PF1131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="site" description="Transition state stabilizer" evidence="3">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005054-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="240" mass="27976" checksum="665D37EF692154F9" modified="2009-02-10" version="1">MRAILILKFDKPLTLPIHYNHILQAAILNLLSDENYSKFIHDSGFQFGKRHFKMFTFSRLEGRFLIDRERKTITYFEKAQLHISTIEDKFIEYVVNNLLLEGIDIRGERIHVDKIELKLNTTNFGKIITKSPIVAYSTFIVNNRKKTYYYNPKEKEFQEILANNLVKKYIAYFGKEPKNKYFEITPVSNLKESIVIYKGTVIKGWNGIFQISGSDELINIAYDTGLGSKNSQGFGCFEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHZ4</accession>
    <name>B7IHZ4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1263</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580101.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1263"/>
    <dbReference type="EnsemblBacteria" id="ACJ75708">
        <property type="protein sequence ID" value="ACJ75708"/>
        <property type="gene ID" value="THA_1263"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1263"/>
    <dbReference type="eggNOG" id="COG4878">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_369162_0_0_0"/>
    <dbReference type="OMA" id="FYYKIWW"/>
    <dbReference type="OrthoDB" id="252303at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018695">
        <property type="entry name" value="DUF2194"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09960">
        <property type="entry name" value="DUF2194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="754" mass="89145" checksum="7607EE4B836473D2" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMS7</accession>
    <name>C0QMS7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_2001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2001"/>
    <dbReference type="EnsemblBacteria" id="ACN29494">
        <property type="protein sequence ID" value="ACN29494"/>
        <property type="gene ID" value="THA_2001"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2001"/>
    <dbReference type="HOGENOM" id="CLU_036902_4_5_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transposase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEDD_Tnp_IS110" evidence="2">
        <location>
            <begin position="20"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="258"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACN29494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="315" mass="36547" checksum="F2C78762885A4CAE" modified="2009-05-05" version="1">MKQKSINKKISRITQDTLIVGIDVAKRTHWIRMMDFRGIDLIKPFKINNTMDGIKMLEEKIKSVKEENKLNKVVLGMEPSGHYWKVLAWQMKISKEIDYLVGVNPYHVKKSKEFDDNSPGKSDRKDAGVIARLIRDGRYFDIYLPEDIYGELRVITTTREQLVNKRKNAKNMVISVMDEYFPEYEKIYKNIFSEGSIKLLKTHPFPKEIIEVGIEEIEKVLKKSTNGKDWKSRAQKIYESAKKSIGVREGQKSAKIKLRMLLEEIEFLTSQIEELEKEMEELVEETEEGEYIKSVPGIGPIMTAVILGEIGDIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMT3</accession>
    <name>C0QMT3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_2007</name>
        <name evidence="4" type="ordered locus">THA_2012</name>
        <name evidence="5" type="ordered locus">THA_2016</name>
        <name evidence="6" type="ordered locus">THA_2018</name>
        <name evidence="7" type="ordered locus">THA_2022</name>
        <name evidence="8" type="ordered locus">THA_2031</name>
        <name evidence="9" type="ordered locus">THA_2033</name>
        <name evidence="10" type="ordered locus">THA_2040</name>
    </gene>
    <organism evidence="3 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 11">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580542.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2007"/>
    <dbReference type="EnsemblBacteria" id="ACN29500">
        <property type="protein sequence ID" value="ACN29500"/>
        <property type="gene ID" value="THA_2007"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29505">
        <property type="protein sequence ID" value="ACN29505"/>
        <property type="gene ID" value="THA_2012"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29509">
        <property type="protein sequence ID" value="ACN29509"/>
        <property type="gene ID" value="THA_2016"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29511">
        <property type="protein sequence ID" value="ACN29511"/>
        <property type="gene ID" value="THA_2018"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29515">
        <property type="protein sequence ID" value="ACN29515"/>
        <property type="gene ID" value="THA_2022"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29524">
        <property type="protein sequence ID" value="ACN29524"/>
        <property type="gene ID" value="THA_2031"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29526">
        <property type="protein sequence ID" value="ACN29526"/>
        <property type="gene ID" value="THA_2033"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29533">
        <property type="protein sequence ID" value="ACN29533"/>
        <property type="gene ID" value="THA_2040"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2007"/>
    <dbReference type="KEGG" id="taf:THA_2012"/>
    <dbReference type="KEGG" id="taf:THA_2016"/>
    <dbReference type="KEGG" id="taf:THA_2018"/>
    <dbReference type="KEGG" id="taf:THA_2022"/>
    <dbReference type="KEGG" id="taf:THA_2031"/>
    <dbReference type="KEGG" id="taf:THA_2033"/>
    <dbReference type="KEGG" id="taf:THA_2040"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_27_6_0"/>
    <dbReference type="OMA" id="FTSMEWA"/>
    <dbReference type="OrthoDB" id="1637053at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025948">
        <property type="entry name" value="HTH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13276">
        <property type="entry name" value="HTH_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13333">
        <property type="entry name" value="rve_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="214"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACN29500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACN29505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACN29509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACN29511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACN29515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACN29524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACN29526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACN29533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="376" mass="44919" checksum="2849D1D4366556FF" modified="2009-05-05" version="1">MAKKGQKFKKYPSEIKYQVVKLFIEENLPKKTIASLLGIPPDRVRLWIKNYLLYGDAERKRGKPKKESFEEEMERLRAENAYLKLLMEKILEEGNSKKKIEVINELKERFSLELLCKISGISKSTYYRAMKRKDKDEKLKEEIKDIYFKYKGIYGYRRITMVLNRKGRKINHKKVYRLMKEMGLYARIRKKNHIRKLTLDAQYIADNKLKRNFKSKRPFEKFVSDITQIKTIDGKLYLIIIQDLYNNEIIEYSIGENNNLELVNNTIKRWLKSGKYRKGGILHTDRGFQYTHVLTAKILKEKGIIQSMSRKGVPQDNGPAESFFSHLKEEVVRINGEKTKEEYKKLIEEYIRFYNEERYQARLKNMAPVEYRNHVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICJ8</accession>
    <name>B7ICJ8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Extradiol ring-cleavage dioxygenase, class III enzyme, subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580114.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1280"/>
    <dbReference type="EnsemblBacteria" id="ACJ75725">
        <property type="protein sequence ID" value="ACJ75725"/>
        <property type="gene ID" value="THA_1280"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1280"/>
    <dbReference type="eggNOG" id="COG2078">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095686_1_1_0"/>
    <dbReference type="OMA" id="WAIEVIE"/>
    <dbReference type="OrthoDB" id="1812209at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027623">
        <property type="entry name" value="AmmeMemoSam_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="PTHR13016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="SSF143447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04335">
        <property type="entry name" value="AmmeMemoSam_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00296">
        <property type="entry name" value="TIGR00296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="1">
        <location>
            <begin position="2"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="173" mass="19465" checksum="066766C29A0D25DC" modified="2009-02-10" version="1">MIGTHPYVKWAIEVIENYILHSKVIEPDPNSLPKELFEKRAGCFVTLHTKNGNLRGCIGTFEPTQENLAFEIRNNAIAAATQDPRFPPVSKGELDNIVVSVDVLSEIQPVSSISELDPKKYGIIVAKGFRRGLLLPDIEGVDTIEEQIRIAKLKAGIFDDDFKIFKFTVERYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICM3</accession>
    <name>B7ICM3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580136.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1305"/>
    <dbReference type="EnsemblBacteria" id="ACJ75750">
        <property type="protein sequence ID" value="ACJ75750"/>
        <property type="gene ID" value="THA_1305"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1305"/>
    <dbReference type="eggNOG" id="COG3481">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056349_2_0_0"/>
    <dbReference type="OMA" id="NLVGHLV"/>
    <dbReference type="OrthoDB" id="2018439at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="178"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="337" mass="38720" checksum="BC426147715A547B" modified="2009-02-10" version="1">MKLPKELIEKFREPYISELENYINSEVETIVKVKSKRLLETKNGQKYLLLTFEDKTGILRAIDWFNAEKNDAKINVGNVVRTQGKVVIYDGRIQLNILEDSNIIVLNEDEYNIERFVSESKMSSESAKTKINYFIDSIKNDTLKKLLIEIFKNDEKLSEAFMSAPAGLRVHHNYVGGLAEHSITVASICENVAKIYTWLDRDLLITGALLHDIGKIYDYEISSHGIELTQKGELIGHIVSGFDIVKEKLNKINASSELSEKLLHMILSHHGEFEWGSPVLPKTPEAYVLHMIENMDSKLNRMYNIKEKELEINPEKTWSDFDPSLGRRILLKHDERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICN2</accession>
    <name>B7ICN2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rpmA</name>
        <name evidence="6" type="ordered locus">THA_1314</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the bacterial ribosomal protein bL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101635.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1314"/>
    <dbReference type="EnsemblBacteria" id="ACJ75759">
        <property type="protein sequence ID" value="ACJ75759"/>
        <property type="gene ID" value="THA_1314"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1314"/>
    <dbReference type="eggNOG" id="COG0211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095424_4_1_0"/>
    <dbReference type="OMA" id="GKDHTLH"/>
    <dbReference type="OrthoDB" id="1904463at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00539">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001684">
        <property type="entry name" value="Ribosomal_L27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018261">
        <property type="entry name" value="Ribosomal_L27_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893">
        <property type="entry name" value="PTHR15893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01016">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00063">
        <property type="entry name" value="RIBOSOMALL27"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00062">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00831">
        <property type="entry name" value="RIBOSOMAL_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="88" mass="9953" checksum="EAE515785ACCDC1A" modified="2009-02-10" version="1">MKINIQLFAKSSTAKNGRDSNPKYLGVKRGDGQKVLAGTIIVRQRGTKFWPGKNVGMGRDFTLFALKDGVVKFEVRNNRKYVSVYEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICT5</accession>
    <name>B7ICT5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1367</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580183.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1367"/>
    <dbReference type="EnsemblBacteria" id="ACJ75812">
        <property type="protein sequence ID" value="ACJ75812"/>
        <property type="gene ID" value="THA_1367"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1367"/>
    <dbReference type="eggNOG" id="COG1729">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2774589_0_0_0"/>
    <dbReference type="OMA" id="KAYEMFV"/>
    <dbReference type="OrthoDB" id="2208557at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="69" mass="8634" checksum="B0C9922B19752600" modified="2009-02-10" version="1">MQRFYLILATIFAFLFLLTFFHLKSLDNRLEYLQKLNKAYDMYVNKDIRFKEYIENNNLDELKYLLERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICU3</accession>
    <name>B7ICU3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Integral membrane protein, Rhomboid family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1375</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580189.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1375"/>
    <dbReference type="EnsemblBacteria" id="ACJ75820">
        <property type="protein sequence ID" value="ACJ75820"/>
        <property type="gene ID" value="THA_1375"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1375"/>
    <dbReference type="eggNOG" id="COG0705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055068_3_0_0"/>
    <dbReference type="OMA" id="IGWEHFL"/>
    <dbReference type="OrthoDB" id="2053476at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002610">
        <property type="entry name" value="Peptidase_S54_rhomboid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936">
        <property type="entry name" value="PTHR22936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="SSF144091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Rhomboid" evidence="6">
        <location>
            <begin position="46"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="223" mass="25304" checksum="4F8835C59E8807A5" modified="2009-02-10" version="1">MKRESFYYLIFVNVLILFLIEIFKFFIPNEAVLYLMFGAQYGPLVSHGQWFRIVTSMFVHGGFIHLIFNMYALFFLGRIVENVYGTEKFLTFYFLSGIVGNLATQIFYYNSFSVGASGAIFGLIGVLFAAGFRKDTPYSLKPITGSALLPMIVINIIFGIMPGTNINNAAHIGGFLTGMLLGYMIPLYDYSWKVRKIWKVIMWILILLVVVSYISLIIWGVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICV1</accession>
    <name>B7ICV1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580195.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1383"/>
    <dbReference type="EnsemblBacteria" id="ACJ75828">
        <property type="protein sequence ID" value="ACJ75828"/>
        <property type="gene ID" value="THA_1383"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1383"/>
    <dbReference type="eggNOG" id="COG2143">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1239653_0_0_0"/>
    <dbReference type="OMA" id="SCYYCKL"/>
    <dbReference type="OrthoDB" id="1398962at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13098">
        <property type="entry name" value="Thioredoxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="8"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="222" mass="26085" checksum="5A64394B102AE981" modified="2009-02-10" version="1">MKKFIFIFLVLATMISFSYDYTMYDLGIAHKISVIEDKPLIIYFSSPSCVYCKKFESEVLSDEKFQELLKLYYTFVKVEPNNNKTIFLENEYTNNELFGAFGVRGTPTFVFWYKDAGITMVPGFMPLEDFLNALNYILKYVYEDYREDFQTFLDNKESFNPKTKVINISNNDADFVLAHDKNAKKYTSDQKIEKGTVYLVYSKEDLEKLKQSGVFRILFVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICX3</accession>
    <name>B7ICX3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Phosphomannomutase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">THA_1406</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580208.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1406"/>
    <dbReference type="EnsemblBacteria" id="ACJ75850">
        <property type="protein sequence ID" value="ACJ75850"/>
        <property type="gene ID" value="THA_1406"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1406"/>
    <dbReference type="eggNOG" id="COG1109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_0_0_0"/>
    <dbReference type="OMA" id="YKAYWEE"/>
    <dbReference type="OrthoDB" id="637615at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016868">
        <property type="term" value="F:intramolecular transferase activity, phosphotransferases"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="8">
        <location>
            <begin position="2"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="9">
        <location>
            <begin position="155"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="10">
        <location>
            <begin position="259"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="7">
        <location>
            <begin position="397"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="476" mass="53436" checksum="2021DD0AC229E29D" modified="2009-02-10" version="1">MILFGTGGIRGVMREGEFDEKTVMVASKGVSNYMKDNNLNKVIIAYDTRNNSRKFAELAAQVFSADNHEVKIFDRPVPTPVLSFAVRYLKYDMGVVITASHNPPEYNGYKVYTSNGVQAVPSVTNVLSRYVENAWNLPINLSENYEFVGDNILNVYIDKVVELISTDLSGLKVLYTPLHGTGAFPVVKVLEKLGAEVIKVEEQMVPDGNFPTVTTPNPEDDEALVLLYKYQKQYGADLAIATDPDCDRVGVVWKGKRLTGNQVGVLIADYLLKDADESSMIVKTIVTTNMVKPICDEKGVKLFETPTGFKFIGDLAEKSSYKFLFGFEESCGYLTGDIARDKDAVIGSALIASVAKHYDLIERLNHLYEKYGYYFEKLLNFKFKELSIAKKLYEKIKAGNIPFDANIVDYSKGYKDIEPNETIKLEFEDASIFVRPSGTEPKLKAYVMVHSDNREYAEKRLQELSDSFSSFIESMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICY2</accession>
    <name>B7ICY2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methylmalonyl-CoAmutase, subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1415</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580211.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1415"/>
    <dbReference type="EnsemblBacteria" id="ACJ75859">
        <property type="protein sequence ID" value="ACJ75859"/>
        <property type="gene ID" value="THA_1415"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1415"/>
    <dbReference type="eggNOG" id="COG2185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128233_0_0_0"/>
    <dbReference type="OMA" id="VYTGLHQ"/>
    <dbReference type="OrthoDB" id="1335734at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006159">
        <property type="entry name" value="Acid_CoA_mut_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00640">
        <property type="entry name" value="acid_CoA_mut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="3">
        <location>
            <begin position="4"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="132" mass="14335" checksum="575B64910B4F92A0" modified="2009-02-10" version="1">MEKKIRVLIAKPGLDGHDRGAKVVSKALRDAGMEVIYTGLRQTPEQIVKAAIQEDVDVIGLSILSGAHMSICKKVLDLMKENDIENIPVFVGGIIPEDDARELKKMGIKEVFGPGTPLSTIVEKIKGLFAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID14</accession>
    <name>B7ID14_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1448</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580228.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1448"/>
    <dbReference type="EnsemblBacteria" id="ACJ75891">
        <property type="protein sequence ID" value="ACJ75891"/>
        <property type="gene ID" value="THA_1448"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1448"/>
    <dbReference type="eggNOG" id="COG1313">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062674_0_1_0"/>
    <dbReference type="OMA" id="CIYCQNM"/>
    <dbReference type="OrthoDB" id="1141206at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040085">
        <property type="entry name" value="MJ0674-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43075">
        <property type="entry name" value="PTHR43075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01099">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="52"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="287" mass="32605" checksum="35F09BCA6C3B9D52" modified="2009-02-10" version="1">MLERCILCPRKCKVNRYEKKGICGADYKVRVSNILLHKGEEPPISGENGAGIIFFSGCPMKCIYCQNMGFSQKGIGKEISIEKLAEAFLTLQKNGAETLDLVTATQYVLQVIEALKIAKNKGFNLPVVYNTSSFENVETLKMLEGYVDIYLADIRYTNSMYGEKYSKVQNYWEIAQKAIKEMYRQVGAFSQENKKGVIIRILVLPNNVSGHFKALRFIAELDPKIPVSLMSQYLPHFGAKNDPLIGRKITKAEYIEAVEYMELLGLNGWIQLDEKDRLTTKAVNFKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID66</accession>
    <name>B7ID66_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1502</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102008.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1502"/>
    <dbReference type="EnsemblBacteria" id="ACJ75943">
        <property type="protein sequence ID" value="ACJ75943"/>
        <property type="gene ID" value="THA_1502"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1502"/>
    <dbReference type="eggNOG" id="ENOG50309QR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119095_0_0_0"/>
    <dbReference type="OMA" id="IVITIAD"/>
    <dbReference type="OrthoDB" id="2058575at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="188" mass="21624" checksum="8C7D42F73264E6F6" modified="2009-02-10" version="1">MKKIENLFEITLKEKSGGWFFWFFLLFIPHLNLFSIFFISNLLLASKIKNKKDKSFYYLPFTKKDVFFILYFFLILLIFSTSFFNSLLISRYSLKYILSPLIFSTAIYSIGIIASSLNGDNFAITILFIIFDAILSNLGSSHLGANFNPYILISPIRQESLIGSILFSLALLFIAFKLYVGGESYVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID80</accession>
    <name>B7ID80_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1516</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102045.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1516"/>
    <dbReference type="EnsemblBacteria" id="ACJ75957">
        <property type="protein sequence ID" value="ACJ75957"/>
        <property type="gene ID" value="THA_1516"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1516"/>
    <dbReference type="HOGENOM" id="CLU_2511606_0_0_0"/>
    <dbReference type="OMA" id="NEYNTQK"/>
    <dbReference type="OrthoDB" id="2063642at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007838">
        <property type="entry name" value="Cell_div_ZapA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05164">
        <property type="entry name" value="ZapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="86" mass="10566" checksum="23A8461B4C754730" modified="2009-02-10" version="1">MIKKRFNINGRNYIVESDSDEKILDYIEKRIKELNEKYEELSSTDERFLVMLCELIEREYYLTEKINEILKRLNDLEERSLEDRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDA3</accession>
    <name>B7IDA3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1539"/>
    <dbReference type="EnsemblBacteria" id="ACJ75980">
        <property type="protein sequence ID" value="ACJ75980"/>
        <property type="gene ID" value="THA_1539"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1539"/>
    <dbReference type="HOGENOM" id="CLU_1659884_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="159" mass="17873" checksum="94B887FB0E637B06" modified="2009-02-10" version="1">MAVLAFIAGLSFRKRHLGQCKNYLPINLIILGCAESVFINIVNCCPSNIYVICAFSVFSFIPLIFILCFSLSIYHSISHLLLYINLFISLSINSFPFLLLGLTYNFIFSFIFSINSHCQYPLSANNIKPSISSNIISAWSTSLLFPISKSNNTFASYNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDE5</accession>
    <name>B7IDE5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Iron uptake regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1584</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fur family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102326.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1584"/>
    <dbReference type="EnsemblBacteria" id="ACJ76022">
        <property type="protein sequence ID" value="ACJ76022"/>
        <property type="gene ID" value="THA_1584"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1584"/>
    <dbReference type="eggNOG" id="COG0735">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_4_2_0"/>
    <dbReference type="OMA" id="WFDTNTS"/>
    <dbReference type="OrthoDB" id="1630127at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="136" mass="15881" checksum="289D54A3765850A7" modified="2009-02-10" version="1">MKRYAEMLKERNIRPTVQRIEILKFIVESKRHPSADEIYEHLKGKIHAISRATVYNTVSLLSEKKVIKEIITPSSIRYDFELVEHHHFYCTKCKKVYDIFESLPEVPKIDSVEGHKVMNIQYCLVGICKDCLNGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDG1</accession>
    <name>B7IDG1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">6-carboxy-5,6,7,8-tetrahydropterin synthase</fullName>
            <ecNumber evidence="8">4.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">queD</name>
        <name evidence="11" type="ordered locus">THA_1600</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + 2 H(+) + triphosphate</text>
            <dbReference type="Rhea" id="RHEA:27966"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="EC" id="4.1.2.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8 10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PTPS family. QueD subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.-.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580288.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1600"/>
    <dbReference type="EnsemblBacteria" id="ACJ76038">
        <property type="protein sequence ID" value="ACJ76038"/>
        <property type="gene ID" value="THA_1600"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1600"/>
    <dbReference type="eggNOG" id="COG0720">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111016_1_2_0"/>
    <dbReference type="OMA" id="KCEKLHG"/>
    <dbReference type="OrthoDB" id="1770189at2"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070497">
        <property type="term" value="F:6-carboxy-5,6,7,8-tetrahydropterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PTHR12589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006113">
        <property type="entry name" value="PTP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03367">
        <property type="entry name" value="queuosine_QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 10" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="23"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006113-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR006113-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ76038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="120" mass="14185" checksum="D5FFC3AA682B3B1F" modified="2009-02-10" version="1">MLLVSKEFTFDAAHNLVKYHGKCEKLHGHTYKLVVTVAGEKDEEGMVIDFLELKRIVQEKVLNRLDHSYINEIIPQPSAENIAEWIWKQLETELHSERYFLYEIKVYETPTSYVTLKKNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDG3</accession>
    <name>B7IDG3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TRK system potassium uptake protein TrkA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1602</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102356.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1602"/>
    <dbReference type="EnsemblBacteria" id="ACJ76040">
        <property type="protein sequence ID" value="ACJ76040"/>
        <property type="gene ID" value="THA_1602"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1602"/>
    <dbReference type="eggNOG" id="COG0569">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046525_2_0_0"/>
    <dbReference type="OMA" id="QRCNVVI"/>
    <dbReference type="OrthoDB" id="1768241at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="2">
        <location>
            <begin position="137"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="219" mass="24382" checksum="DC0A9900FF0A95A2" modified="2009-02-10" version="1">MKVVLIGGEKIAYFLAKSFSSKGYKTYLVNKDQKLCEDFARDLKAVVIHGDASKKRLLEQLDIEEDDIIVVLTNKDKENLIITQFAKKIFGVKNIVTLVNNPDNIELFEKLGITAVVSTTTMLQKAVENLLFGEELQQFLSVEEGKLTFLKIDIPENSKAVGKLLKDLNLPHDCVVGGILRKGKVIIPHGNTEINAGDRLYIVSLPTAQEQILDILMGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDI2</accession>
    <name>B7IDI2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidate cytidylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cdsA</name>
        <name evidence="2" type="ordered locus">THA_1621</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102403.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1621"/>
    <dbReference type="EnsemblBacteria" id="ACJ76059">
        <property type="protein sequence ID" value="ACJ76059"/>
        <property type="gene ID" value="THA_1621"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1621"/>
    <dbReference type="eggNOG" id="COG4589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037294_2_1_0"/>
    <dbReference type="OMA" id="FTNDECS"/>
    <dbReference type="OrthoDB" id="1533756at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="270" mass="30488" checksum="F2365F8A20CE8DFE" modified="2009-02-10" version="1">MTKETKVRLISAFIVAPFVVACFISYQSLIGLVSAIVLLASSELLFATLKKHRKNGILVVYIALLSAFPLFFGIWFLEQPMELLSVLYIIGIVFTLFIVKNKEIVMEFFGVYSISFIYISMNLSFFIPLYKFYGAAVALLTLTLSWAYDSFAYFFGLSFGRHKLSKVYSPNKSYEGLLGGIFGTFVYTLIYFVIINSFFNYSIPLWYSIAFAIITGIMDTAGDIFESAIKRAYGLKNIGRFMPGHGGMLDRIDGLLFVAPVIYIFLKLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDK3</accession>
    <name>B7IDK3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1642</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580320.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1642"/>
    <dbReference type="EnsemblBacteria" id="ACJ76080">
        <property type="protein sequence ID" value="ACJ76080"/>
        <property type="gene ID" value="THA_1642"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1642"/>
    <dbReference type="eggNOG" id="COG1386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045647_5_3_0"/>
    <dbReference type="OMA" id="FYTKPEY"/>
    <dbReference type="OrthoDB" id="1771906at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051304">
        <property type="term" value="P:chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005234">
        <property type="entry name" value="ScpB_csome_segregation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34298">
        <property type="entry name" value="PTHR34298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04079">
        <property type="entry name" value="SMC_ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="181" mass="20716" checksum="FD4F92CCE25A65A1" modified="2009-02-10" version="1">MEKEKIALIEAIIFASKGISIQKLSELTNIDQDEINKIIKHLYSKYNNNSESGIELKNIDGYLKFYTKKQYSSYIEKVVKRRSLSTLTNQQLEIVILLATKKEATKKEIDGIRGKDSTNILKQLLYSGVIKRKKSGRSYIYGLTETFKEETLIEELIEELGGAQLDTFRSKNSSPQNNGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDL2</accession>
    <name>B7IDL2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transglycosylase SLT domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102456.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1651"/>
    <dbReference type="CAZy" id="GH23">
        <property type="family name" value="Glycoside Hydrolase Family 23"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76089">
        <property type="protein sequence ID" value="ACJ76089"/>
        <property type="gene ID" value="THA_1651"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1651"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101726_0_0_0"/>
    <dbReference type="OMA" id="ELGMMQI"/>
    <dbReference type="OrthoDB" id="1444566at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLT" evidence="1">
        <location>
            <begin position="52"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="178" mass="20801" checksum="2B94A51527653ECB" modified="2009-02-10" version="1">MKKHFLIFLLLTIAISTFALPGKWFRDMIKYYREAYNLPCDEELIQNIENAILDASKETGIDPLLITSVIIVETEFRNVIGMHGELGMMQIKPETAEFVANLYKISTPQEGWNRILWDFKLNIKIGSYYLKYLYDKFHNITDAVKHYNGGTYKDQYAQKILAKYKEMLKVASINERNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDM5</accession>
    <name>B7IDM5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2Fe-2S iron-sulfur cluster binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1664</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580334.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1664"/>
    <dbReference type="EnsemblBacteria" id="ACJ76102">
        <property type="protein sequence ID" value="ACJ76102"/>
        <property type="gene ID" value="THA_1664"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1664"/>
    <dbReference type="eggNOG" id="COG2080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052511_3_1_0"/>
    <dbReference type="OMA" id="RSCVIPL"/>
    <dbReference type="OrthoDB" id="1843415at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47741">
        <property type="entry name" value="SSF47741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="154" mass="16828" checksum="7FE48D16EF9073C9" modified="2009-02-10" version="1">MKIKFTLNGQTIERDVPVYKRALDFLRDDLKITSVKEGCGEGECGACTIIVNGKNVHSCLMLAVELDGKEIITLEGIEDQIKDAYVEAGAIQCGFCTPGFIVSTKVLLDKHPNPSEEEIKEALEGNLCRCTGYVKIINAVKIASSYIKGSEKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDP4</accession>
    <name>B7IDP4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH-ubiquinone oxidoreductase 49 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1684</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102511.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1684"/>
    <dbReference type="EnsemblBacteria" id="ACJ76121">
        <property type="protein sequence ID" value="ACJ76121"/>
        <property type="gene ID" value="THA_1684"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1684"/>
    <dbReference type="eggNOG" id="COG0649">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_1_2_0"/>
    <dbReference type="OMA" id="GGRMHYM"/>
    <dbReference type="OrthoDB" id="473681at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014029">
        <property type="entry name" value="NADH_UbQ_OxRdtase_49kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="PTHR11993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00535">
        <property type="entry name" value="COMPLEX1_49K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0874">Quinone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 5" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Complex1_49kDa" evidence="6">
        <location>
            <begin position="118"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="367" mass="41907" checksum="44CCA7D4632BA5CE" modified="2009-02-10" version="1">MGEVKLFFGPNHPGMHGNFSVHLYVEGDTVIRARPMPGFLHRGFEKLMERRLWYQNLALIPRICVPEPDINEVVYALAVEKLANVEVPERAQWIRMIVLELARIAVHLMSMAGIGGPIGLYTGPYWGVADRDRILDIFENLTGARIYHMYIIPGGVRKDMTPKVQDMIVKFLDYLESRLPEFENLIFKNRIVQTRLKGIALLDRETAFEMGVTGIGLRATGIPYDIRKIDPYLFYDKVEFEVPTATEGDAFSRVYLKFKEIPQSIRIIRQCLEKMPEGKINVPISEGNALRYRVPKGSVYVHIEASRGEYGYYVVSDGGEKPYRIVVRGASYPQTLYGIEKYLPGTRIEDVPIWLDTMGVCSPEVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDS1</accession>
    <name>B7IDS1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Motility protein PilT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1712</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102570.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1712"/>
    <dbReference type="EnsemblBacteria" id="ACJ76148">
        <property type="protein sequence ID" value="ACJ76148"/>
        <property type="gene ID" value="THA_1712"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1712"/>
    <dbReference type="eggNOG" id="COG2805">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013446_4_0_0"/>
    <dbReference type="OMA" id="WELDCSY"/>
    <dbReference type="OrthoDB" id="473670at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01131">
        <property type="entry name" value="PilT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006321">
        <property type="entry name" value="PilT/PilU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01420">
        <property type="entry name" value="pilT_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00662">
        <property type="entry name" value="T2SP_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="2">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="353" mass="39485" checksum="128D81866F2D7ECB" modified="2009-02-10" version="1">MNELNIDEIILKANQSRASDVHISANMPIMLRVDGNLVKLPGENIPTAQEIEIMVENLFSKLGINSLKKEIDFSFSIHNIRIRANFYYEKRNPTLALRLIPKKIRTIEELGLPKILNEFSEKDHGLILVAGPTGSGKSTTLAAMIEHINSRFAKHIITIEDPIEYVFDSKRSLIHQREVGTDTDSFSDGLKYALRQDPDVILVGEMRDLETMSLALTAAETGHLVFATIHTNSAASAPERIIDVFPAHQQKQIALQLANTLIAVIFQRLVPKKDFGVVAVDEIMIATPAIKNLIRENKLHQIEGVMQTSKKQGNILFDDALIDAFLKGVITKQAVLENARNIEEVSKKIGWRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDT0</accession>
    <name>B7IDT0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580371.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1721"/>
    <dbReference type="EnsemblBacteria" id="ACJ76157">
        <property type="protein sequence ID" value="ACJ76157"/>
        <property type="gene ID" value="THA_1721"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1721"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110169_0_0_0"/>
    <dbReference type="OMA" id="YTVARQD"/>
    <dbReference type="OrthoDB" id="1291710at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041561">
        <property type="entry name" value="PglD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17836">
        <property type="entry name" value="PglD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PglD_N" evidence="1">
        <location>
            <begin position="116"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="208" mass="23663" checksum="F4EBEDAFCC320BF6" modified="2009-02-10" version="1">MIKIDTLKFFQPTPEYRKLVILEALYENPNLSQKELSAIAGVVPSLVNKYINELEKKGYIETKDLGKNKIYNVTGEGLSELYFLKLSFFNDIVNLSKRIDSQLENIFLKLKGKNEIGIYGAGLVGKVLAELLLNRGYNIVVFFDDDNGKVNSRVFGIPILPLNSKVRIDALVVASIKNSEQMVKKAKKFDFTDIYVFKTEEFKLSWHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDW0</accession>
    <name>B7IDW0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CobQ/CobB/MinD/ParA nucleotide binding domain, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1753</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580389.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1753"/>
    <dbReference type="EnsemblBacteria" id="ACJ76187">
        <property type="protein sequence ID" value="ACJ76187"/>
        <property type="gene ID" value="THA_1753"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1753"/>
    <dbReference type="eggNOG" id="COG0455">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_0_0_0"/>
    <dbReference type="OMA" id="FKVAKNN"/>
    <dbReference type="OrthoDB" id="1370117at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="277" mass="31247" checksum="CC6D46A22786340A" modified="2009-02-10" version="1">MLNQASNLINLENVVLIGSGKGGVGKTLITVNLAIVLKQLGYKVLIFDLDVGFTNSDVLLNVHPQYSINDLLLNKCEREDVICSTEYGVDLVSVGSDIESIFNFNPENIKNFYIKFAQIAKDYDFTLIDLPPGYNNNYAPFFNSASHTITITTTHPTSLVNSYTFVKILIHKGVSSSNIHLVGNNVENYNESLENLKRFSSVLEKFTGEKMGSLTIIKRHNLVEKSVFNRKPFVIDHPKIQPTFALYRIASILTKKDILYKENILDKILSFFRSDQE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDX6</accession>
    <name>DNLJ_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase</fullName>
            <ecNumber evidence="1">6.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polydeoxyribonucleotide synthase [NAD(+)]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ligA</name>
        <name type="ordered locus">THA_1772</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.</text>
            <dbReference type="EC" id="6.5.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD-dependent DNA ligase family. LigA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580400.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDX6"/>
    <dbReference type="SMR" id="B7IDX6"/>
    <dbReference type="STRING" id="484019.THA_1772"/>
    <dbReference type="EnsemblBacteria" id="ACJ76203">
        <property type="protein sequence ID" value="ACJ76203"/>
        <property type="gene ID" value="THA_1772"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1772"/>
    <dbReference type="eggNOG" id="COG0272">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007764_2_1_0"/>
    <dbReference type="OMA" id="HDVEHEI"/>
    <dbReference type="OrthoDB" id="241401at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003911">
        <property type="term" value="F:DNA ligase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00114">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01588">
        <property type="entry name" value="DNA_ligase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018239">
        <property type="entry name" value="DNA_ligase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033136">
        <property type="entry name" value="DNA_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001679">
        <property type="entry name" value="DNAligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013839">
        <property type="entry name" value="DNAligase_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013840">
        <property type="entry name" value="DNAligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004150">
        <property type="entry name" value="NAD_DNA_ligase_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004149">
        <property type="entry name" value="Znf_DNAligase_C4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01653">
        <property type="entry name" value="DNA_ligase_aden"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03120">
        <property type="entry name" value="DNA_ligase_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03119">
        <property type="entry name" value="DNA_ligase_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001604">
        <property type="entry name" value="LigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00532">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="SSF52113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00575">
        <property type="entry name" value="dnlj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01055">
        <property type="entry name" value="DNA_LIGASE_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01056">
        <property type="entry name" value="DNA_LIGASE_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000380492" description="DNA ligase">
        <location>
            <begin position="1"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="1">
        <location>
            <begin position="587"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="1">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="34"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="83"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01588"/>
        </source>
    </evidence>
    <sequence length="665" mass="75663" checksum="324C663D0163011C" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDY1</accession>
    <name>B7IDY1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UPF0313 protein THA_1777</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1777</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UPF0313 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580402.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1777"/>
    <dbReference type="EnsemblBacteria" id="ACJ76208">
        <property type="protein sequence ID" value="ACJ76208"/>
        <property type="gene ID" value="THA_1777"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1777"/>
    <dbReference type="eggNOG" id="COG1032">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018288_2_0_0"/>
    <dbReference type="OMA" id="TCMYYTG"/>
    <dbReference type="OrthoDB" id="229299at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01251">
        <property type="entry name" value="UPF0313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022946">
        <property type="entry name" value="UPF0313"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013704">
        <property type="entry name" value="UPF0313_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32331">
        <property type="entry name" value="PTHR32331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08497">
        <property type="entry name" value="Radical_SAM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01069">
        <property type="entry name" value="UPF0313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03904">
        <property type="entry name" value="SAM_YgiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="287"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="556" mass="64130" checksum="C51271A72725650A" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE53</accession>
    <name>B7IE53_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1849</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102818.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1849"/>
    <dbReference type="EnsemblBacteria" id="ACJ76280">
        <property type="protein sequence ID" value="ACJ76280"/>
        <property type="gene ID" value="THA_1849"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1849"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_3_1_0"/>
    <dbReference type="OMA" id="WYNTPWR"/>
    <dbReference type="OrthoDB" id="1465613at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="4"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="2">
        <location>
            <begin position="142"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="324" mass="37334" checksum="563D019FADC596B6" modified="2009-02-10" version="1">MKKIRLAIVGCGIAARDLHYPALKALNDKFEIVAVTSRTKKHAEEFAKLVGNPEVFDTYEELLEADYIDAVDLTLPIHLNVPFAIKAVEKGKHVICEKPISTDVESAKQLLNVSKETNKVIYIAENWRHIKKFRLIKNLLKNFKEVLYFNWRIFVGMKKDNKYAKTDWRKKPQHIGGFLSDAGVHHVAAMRLIFGDIDWVTAYTKDFSDYLSGPDFISTIVEFKNGVIGNYVASYSFNEEEQFEIYGKENTLKVLKNKIFYNEKEIDFEENMGYKEEFEDFYEVILNNKENDLGNCEEAIKDLAFFEAALKSNGNKIKVDALIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE91</accession>
    <name>B7IE91_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA-uridine 2-sulfurtransferase</fullName>
            <ecNumber evidence="2">2.8.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1892</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47032"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="Rhea" id="RHEA-COMP:11728"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:87170"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.8.1.13" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1892"/>
    <dbReference type="EnsemblBacteria" id="ACJ76318">
        <property type="protein sequence ID" value="ACJ76318"/>
        <property type="gene ID" value="THA_1892"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1892"/>
    <dbReference type="eggNOG" id="COG0482">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035188_0_0_0"/>
    <dbReference type="OMA" id="VHLLCEQ"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="Adenine_a_hdrlase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="tRNA-specific_2-thiouridylase"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="321" mass="36999" checksum="533E0574983C4611" modified="2009-02-10" version="1">MKTVFDEIYLTKKITHKVCCSPSDTYDAKKIAEKFGVDFKVVHLEDDFREMVINYFVSEYKRGKTPNPCFFCNDWIKFGVLMEKMFEDGMDYVASGHYAIIKNGKLYKAKSYEKDQSYFLASIKKEKLKKIILPNGEYTKQQIREIAEKIGIHVHNKVDSQDLCFIPDGNISEFLRENNMEFKEGLIIDTEGNIIGTHKGLANYTIGQRKIGVATGRRLYVIKKDFKNNILIVGDKESAFSTKFTVLKPNFLQNVSKDFEGYVKVRKKFKEVRCKAHFENERLYIETQEPIFAITPGQIAVVYGFDDSVIVSGIIEGEGWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE97</accession>
    <name>B7IE97_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chlorohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580472.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1898"/>
    <dbReference type="EnsemblBacteria" id="ACJ76324">
        <property type="protein sequence ID" value="ACJ76324"/>
        <property type="gene ID" value="THA_1898"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1898"/>
    <dbReference type="eggNOG" id="COG0402">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_2_0_0"/>
    <dbReference type="OMA" id="ALIGKVC"/>
    <dbReference type="OrthoDB" id="1592010at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="1">
        <location>
            <begin position="50"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="413" mass="47192" checksum="B727C0069A67EC04" modified="2009-02-10" version="1">MILRNAYVLRNWKSNIEKVDIKIDKGKIIKIDKNLKDNEDEEFDLDNKLIIPGLVNTHTHAAMSILRGIAEDLSFNKWLFEHIIPLEEKLNEDIVYFASMLSMMEMSKNGIVAFCDMYMFENYVAKAVKDFGMKALLTRGLVSEEDKIEKSRLKEALSLFEKWHGIDNRIFVGLGPHAPYTCSLEALKEIGKLSRKLKIPVTMHLFENKWEKEQFSLSQILSSGIADYHFLAVHCVHLDDNDIKMLKDFNVFISHNPTSNLKLGNGIAPIQKMIENGLTVALGTDGPASNNSLDILFEARIASLLQKKDNPQNLDINQMLKMLTINGYRSLNIEGGEIKEGFPADLTIINLKNPSFYPIENMKKHIIHSKVDVFATMVNGKFTYYNGTFPTIDEKEVYRKFSTFYKKVIGIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEF9</accession>
    <name>B7IEF9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1965</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580512.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1965"/>
    <dbReference type="EnsemblBacteria" id="ACJ76386">
        <property type="protein sequence ID" value="ACJ76386"/>
        <property type="gene ID" value="THA_1965"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1965"/>
    <dbReference type="eggNOG" id="ENOG502Z8GF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_610750_0_0_0"/>
    <dbReference type="OMA" id="PLWWGAT"/>
    <dbReference type="OrthoDB" id="658869at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="435" mass="50198" checksum="66AA2395D64FA2F9" modified="2009-02-10" version="1">MKKYFILILILMSVLALSFTLKVEAGKVEVPFVMKMSDFLDYLPEDVDPYWDAIRVKVEENYVPFQIDDVDGNGRISGDDYLLFLAKGNCEIIIPESEMGVVKFENYFEVIKDGNNWLVSGENGETYKVDDHGLVHVTKYGDVEGTLLDEIGILRVAGFANSTYYVNGELGKHFEEVSYAFEPVSIKVIPGPVAFSVYTKLRSITFSGLDQYVITHVFKNGDILVNNNVFFRNYADLMKLQHMVTRVLTSVDGDALHVLPVFRKLVWADQLNITPYEYWLERNAIKMVDNKPYIVFPAKDSMKPLWWGATYIFASEERWRGNYSEKYKLGIAEILPEIPVVKDNFVDWINGNTWVFESQEFRDGIFKWIPGEFEAFDATKGAYPTEMSSWPNRYYAGQQVNFTRLYSINKSNDLNDWIKFLEVKSDSFRSLKIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEH2</accession>
    <name>B7IEH2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ferrodoxin oxidoreductase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1978</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580523.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1978"/>
    <dbReference type="EnsemblBacteria" id="ACJ76399">
        <property type="protein sequence ID" value="ACJ76399"/>
        <property type="gene ID" value="THA_1978"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1978"/>
    <dbReference type="eggNOG" id="COG1013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048564_0_0_0"/>
    <dbReference type="OMA" id="EHDGFAH"/>
    <dbReference type="OrthoDB" id="981516at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011896">
        <property type="entry name" value="OFOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032686">
        <property type="entry name" value="PFO_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12367">
        <property type="entry name" value="PFO_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02177">
        <property type="entry name" value="PorB_KorB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="2">
        <location>
            <begin position="53"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="PFO_beta_C" evidence="3">
        <location>
            <begin position="200"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="265" mass="29498" checksum="950761FC977F3D91" modified="2009-02-10" version="1">MLNVKLYDIPDADIAWCPGCGNFGILNELKTALSELNLKPNQVVIVSGIGQAAKMPQYVNVNMFNGLHGRTLPVATGIKLTNPSLVVIAESGDGCTYGEGGNHFIHTIRKNPNIVNIVHDNMIYGLTKGQASPTTPKGVKTTLQFDGVYIDPFNPIAVAVALNASFVARTFMGYTELTVELIKKAINHRGYALIDILQPCATFNKYNTYQWYKENTYILPEDYDPTDRKKAFEIAMDTNKLALGLIYKNDSRDVYEDFHPRIELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEI1</accession>
    <name>B7IEI1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sugar abc transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1987</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103060.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1987"/>
    <dbReference type="EnsemblBacteria" id="ACJ76408">
        <property type="protein sequence ID" value="ACJ76408"/>
        <property type="gene ID" value="THA_1987"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1987"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_2_0"/>
    <dbReference type="OMA" id="TIWITAS"/>
    <dbReference type="OrthoDB" id="674327at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="224"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="110"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="426" mass="47988" checksum="DC6B762365C5AA55" modified="2009-02-10" version="1">MRVNKILKQTLFYLVVAGIILVILTPIYFLVVISLMSDQEAYDWPTQLVPSFFNNYKLEIVDGKFLISVYSSAKKKYSTLIKTNDFEELQKFVRNKTNSTIKKEIFDIQVKKLNELISKNSEEISNLRNKIASLESKLNTVTVNLVRYKTQLEKAKKANMNNLISIINEKIIEFENEKREYEKQIKELNERLDEIGSVKFSVSKHLFDNYVNFFRVTSDALPALFRSIQVAILTVLISLTIGGMAGYAFARYNFKGKNLLKLSVLFVRMFPGISIAMPMVIILINMGFYDKPIGLSLVYSVGQIGMTIWITASIFMGISKELEEAAMIFGTTRWGAFFKVTLPLALPGLAASAMYAFIGSWSETAQAIVLTQFNPTFPVVVYQTLVGAKGLINLTAAGGVSLALPAVIFTLIIRRYILQMWGGVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEJ4</accession>
    <name>B7IEJ4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Modification methylase, type III R/M system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_2000</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580537.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2000"/>
    <dbReference type="REBASE" id="19388">
        <property type="entry name" value="M.TafTCORF2000P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76421">
        <property type="protein sequence ID" value="ACJ76421"/>
        <property type="gene ID" value="THA_2000"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2000"/>
    <dbReference type="eggNOG" id="COG2189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013151_1_0_0"/>
    <dbReference type="OMA" id="DFQKKLW"/>
    <dbReference type="OrthoDB" id="161391at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006306">
        <property type="term" value="P:DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_N4_Mtase" evidence="4">
        <location>
            <begin position="413"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="879" mass="105818" checksum="E85781623F9DBE5D" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEK9</accession>
    <name>B7IEK9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_15</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579295.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_15"/>
    <dbReference type="EnsemblBacteria" id="ACJ74523">
        <property type="protein sequence ID" value="ACJ74523"/>
        <property type="gene ID" value="THA_15"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_15"/>
    <dbReference type="eggNOG" id="ENOG5033HZ4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1958525_0_0_0"/>
    <dbReference type="OrthoDB" id="1495676at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="128" mass="15559" checksum="2997263256B7D023" modified="2009-02-10" version="1">MKKIIFILFVIIATTFFSIQFEIKLPIFDKNNGVYQIYTFEHQNKLEVTVVFWDEDHPNSLIDFIYDIYRFFKWGRFYDIETFFILDDKVIFEDDYCNSQSYFQTEKLHNYKELSTNTLYCSPEGLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEL5</accession>
    <name>B7IEL5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_21</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103107.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_21"/>
    <dbReference type="EnsemblBacteria" id="ACJ74529">
        <property type="protein sequence ID" value="ACJ74529"/>
        <property type="gene ID" value="THA_21"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_21"/>
    <dbReference type="HOGENOM" id="CLU_1730551_0_0_0"/>
    <dbReference type="OMA" id="YLYSMSI"/>
    <dbReference type="OrthoDB" id="1671589at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="151" mass="17480" checksum="E6E9E7CC2561D2BD" modified="2009-02-10" version="1">MVVKSRFVIVPMPVMQFVMKARPANAGRAYLYLYSMSIAVGKKVTPWISYRDLADYFGVKYGTIELSMRLLQRLNAIVPIARKNKKGYGQKYIVNVPEYVNGEFIFVDLEESSIFDDLVLDENSKTFVSVIKRFKSRKEKAMKDQLKLFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEN5</accession>
    <name>B7IEN5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_41</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103365.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_41"/>
    <dbReference type="EnsemblBacteria" id="ACJ74549">
        <property type="protein sequence ID" value="ACJ74549"/>
        <property type="gene ID" value="THA_41"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_41"/>
    <dbReference type="eggNOG" id="COG1555">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052011_2_0_0"/>
    <dbReference type="OMA" id="IDYRESH"/>
    <dbReference type="OrthoDB" id="1948240at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004509">
        <property type="entry name" value="Competence_ComEA_HhH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00426">
        <property type="entry name" value="TIGR00426"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="2">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="2">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="2">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="2">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="191" mass="21888" checksum="8C12973DA2C3AC2E" modified="2009-02-10" version="1">MFYGEILNSKIKYFKEFIIIFTLFILISTVIFHEKQYNNVNSKEIKRDYESQIIDLNKADLEQLMSLPGIGTVKAKAIISYRQAHGNFNSIDDLINVTGIGPSTLEKIRDYVTVSKTNEVQINMNNELKKININKADEKQLEKLPGIGPTKAKRIIEYREKNGKFNSLNELLNVNGIGPKTLEKIKNYLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEP6</accession>
    <name>B7IEP6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Actinorhodin polyketide dimerase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_52</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103376.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_52"/>
    <dbReference type="EnsemblBacteria" id="ACJ74560">
        <property type="protein sequence ID" value="ACJ74560"/>
        <property type="gene ID" value="THA_52"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_52"/>
    <dbReference type="eggNOG" id="COG1853">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059021_4_2_0"/>
    <dbReference type="OMA" id="WFDRGYH"/>
    <dbReference type="OrthoDB" id="1681849at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016646">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin_Reduct" evidence="1">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="151" mass="17276" checksum="D5090AC0D32C0BC0" modified="2009-02-10" version="1">MDALSKIYNSVAVVTMNYNGNLNGITVAWIMRTSIDPKLLAISIGKQRYSYELLKKVNFFGINLLSINQINIAKHFGTISGRNFNKFEGIDFKMSKNKIPILNGVVAYLECEKIGDFESGDHNIFLGKVNFEEIFNNEKVLLYGEHKFWRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEQ4</accession>
    <name>B7IEQ4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">tRNA (Guanine-N(1)-)-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_60</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103391.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_60"/>
    <dbReference type="EnsemblBacteria" id="ACJ74568">
        <property type="protein sequence ID" value="ACJ74568"/>
        <property type="gene ID" value="THA_60"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_60"/>
    <dbReference type="eggNOG" id="COG4752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1414575_0_0_0"/>
    <dbReference type="OMA" id="GTGWGMP"/>
    <dbReference type="OrthoDB" id="1276858at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18085">
        <property type="entry name" value="TM1570-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019230">
        <property type="entry name" value="RNA_MeTrfase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09936">
        <property type="entry name" value="Methyltrn_RNA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltrn_RNA_4" evidence="1">
        <location>
            <begin position="4"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="188" mass="21623" checksum="A83A622E6BA4D0AF" modified="2009-02-10" version="1">MLSKVYIALIHYPILGRDGKIISTAVTNLDIHDIARTSRTYKIKKYYLVNNLPAQQDIVKKVLEYWREGFGKEYNPNRSEALSLVELKAYFEDVVEDIERIEREKPIIMFTSAKMRENTISFEAGRRMVLESEKPILILFGTGWGMPNEILSKCDYALEPVRAKSDFNHLSVRAAVAIILDRLIGENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEV6</accession>
    <name>B7IEV6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integrase, catalytic region, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_112</name>
        <name evidence="3" type="ordered locus">THA_375</name>
        <name evidence="4" type="ordered locus">THA_495</name>
        <name evidence="5" type="ordered locus">THA_499</name>
        <name evidence="6" type="ordered locus">THA_553</name>
    </gene>
    <organism evidence="2 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_112"/>
    <dbReference type="EnsemblBacteria" id="ACJ74620">
        <property type="protein sequence ID" value="ACJ74620"/>
        <property type="gene ID" value="THA_112"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ74869">
        <property type="protein sequence ID" value="ACJ74869"/>
        <property type="gene ID" value="THA_375"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ74985">
        <property type="protein sequence ID" value="ACJ74985"/>
        <property type="gene ID" value="THA_495"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ74989">
        <property type="protein sequence ID" value="ACJ74989"/>
        <property type="gene ID" value="THA_499"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75041">
        <property type="protein sequence ID" value="ACJ75041"/>
        <property type="gene ID" value="THA_553"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_112"/>
    <dbReference type="KEGG" id="taf:THA_375"/>
    <dbReference type="KEGG" id="taf:THA_495"/>
    <dbReference type="KEGG" id="taf:THA_499"/>
    <dbReference type="KEGG" id="taf:THA_553"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_31_3_0"/>
    <dbReference type="OMA" id="RMTAQLN"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025948">
        <property type="entry name" value="HTH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13276">
        <property type="entry name" value="HTH_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="100"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="273" mass="32644" checksum="4407C142716CF18A" modified="2009-02-10" version="1">MVKEGFSISEAVRYLKISRSTYYYKPKEYKRKKNNESILKLIQKLKQKHPYWGYRRIYAMFRKQGKKINHKRVYRIMKENGLLLKQKKKKARRVFQSKIIPSRPRQVLGIDMTKVLTKDGGWVNYIAVIDWYTREVLGSQISLRGRTEEWLKALNNALNKGYKDGVRGREVILVSDNGTQPTSRRFMEECKILGIKQIFTSYNNPKGNANTERYFRTYKEEVVWPVEEMSYSELVARTKEYEEFYNSKYPHSALKYRSPREVYEEYLSFTKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF08</accession>
    <name>B7IF08_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_165"/>
    <dbReference type="EnsemblBacteria" id="ACJ74672">
        <property type="protein sequence ID" value="ACJ74672"/>
        <property type="gene ID" value="THA_165"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_165"/>
    <dbReference type="HOGENOM" id="CLU_3334139_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="38" mass="4302" checksum="1C683CBED4F8D646" modified="2009-02-10" version="1">MKVCIVNIFYSSKKLKNVYFSGIINSSIINSKGGKKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF22</accession>
    <name>B7IF22_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Aminomethyltransferase</fullName>
            <ecNumber evidence="3 7">2.1.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Glycine cleavage system T protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 11" type="primary">gcvT</name>
        <name evidence="11" type="ordered locus">THA_181</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(6S)-5,6,7,8-tetrahydrofolate + (R)-N(6)-(S(8)-aminomethyldihydrolipoyl)-L-lysyl-[protein] = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="3 7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579395.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_181"/>
    <dbReference type="EnsemblBacteria" id="ACJ74686">
        <property type="protein sequence ID" value="ACJ74686"/>
        <property type="gene ID" value="THA_181"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_181"/>
    <dbReference type="eggNOG" id="COG0404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_10_2_0"/>
    <dbReference type="OMA" id="MPVQYPA"/>
    <dbReference type="OrthoDB" id="282830at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00259">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018551">
        <property type="entry name" value="DUF2007"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006223">
        <property type="entry name" value="GCS_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022903">
        <property type="entry name" value="GCS_T_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GCST/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GCV_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCV_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="PTHR43757"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09413">
        <property type="entry name" value="DUF2007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="SSF101790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DUF2007" evidence="10">
        <location>
            <begin position="2"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="GCV_T" evidence="8">
        <location>
            <begin position="79"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="GCV_T_C" evidence="9">
        <location>
            <begin position="350"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF09413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ74686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="436" mass="49186" checksum="6FD44B09FFAA278A" modified="2009-02-10" version="1">MWKKLLEVDGEINARLIEGMLKTNNIDCVIKSADEPFPKAYFGSTSFFEIYVPKDLFDEANNLLNSFKKEELYMKYTPLYEQHVKLGAKMVEFAGFQMPIQYTGIKDEVIAVRKDVGMFDVSHMGQVLVEGKDSTNFVNYLITNDFKNLSNGEIVYTAMCNENGGFIDDLLAYKISDEKAFLVINAANIEKDFEWMKNVALNFDVKLENKSDDYALIAIQGPNAQKTLQKLTDIDLESIGYYTFVFGKVKDVEALISRTGYTGEDGFEIYTTDKDGIVKIWEELLNLGVKPAGLGARDTLRLEASLLLYGNDMDETVTPLEAGIKWAVKFDKEFVGKAALEKQLEEGLKRRLKGFKLIDKGIARHGYKVFKDGREIGVVTSGTFSPTLDESIGMALIETGYKSGDIIEIEIRNKLVKAEIVKMPFYRGSVRSKKKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF24</accession>
    <name>GCSPB_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable glycine dehydrogenase (decarboxylating) subunit 2</fullName>
            <ecNumber evidence="1">1.4.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glycine cleavage system P-protein subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycine decarboxylase subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycine dehydrogenase (aminomethyl-transferring) subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gcvPB</name>
        <name type="ordered locus">THA_183</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-N(6)-lipoyl-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = (R)-N(6)-(S(8)-aminomethyldihydrolipoyl)-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvP family. C-terminal subunit subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579397.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IF24"/>
    <dbReference type="SMR" id="B7IF24"/>
    <dbReference type="STRING" id="484019.THA_183"/>
    <dbReference type="EnsemblBacteria" id="ACJ74688">
        <property type="protein sequence ID" value="ACJ74688"/>
        <property type="gene ID" value="THA_183"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_183"/>
    <dbReference type="eggNOG" id="COG1003">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004620_5_0_0"/>
    <dbReference type="OMA" id="MHINLHK"/>
    <dbReference type="OrthoDB" id="70707at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00713">
        <property type="entry name" value="GcvPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023012">
        <property type="entry name" value="GcvPB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="PTHR11773"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000132505" description="Probable glycine dehydrogenase (decarboxylating) subunit 2">
        <location>
            <begin position="1"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00713"/>
        </source>
    </evidence>
    <sequence length="480" mass="53775" checksum="7EF963D14E1D6A11" modified="2009-02-10" version="1">MTIFEISKEGRIGFQLPDNNIKDYEINLPEHLLRKSLPRLPQVSEVDVVRHYTNLSAKNYSVDVGFYPLGSCTMKYNPKVNEKVASFEEFSMIHPFQPHETVQGALKLMYDLKEMLCEITGMDDMTLIPSAGAHGELTGILISRAYHLSRGDTKRTKVIVPDSAHGTNPASARMAGFDVIEIKSGPDGRVDLNELEKVLDETVAVIMLTNPNTLGLFEKDILKIAQMAHDKGALLYYDGANLNAILGRTRPGDMGFDIVHLNLHKTFSTPHGMGGPGSGPVGVKKHLAKFLPVPEIVKIEDKYTLNYNKPESIGFIRSYFGNFSVMVRAYTYIKTMGKDGLKKAGEMAVLNANYLRVKVSKIMDIAYPGICMHEFVSTCEKLTKETGVKALDIAKRLLDYGIHAPTMYFPLIVHEDFMIEPTETESKDTLDKFAQILEKIFNEARENPELVKGAPYNTPVRRLDDVSASRKPVFKYNFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF40</accession>
    <name>B7IF40_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein translocase subunit SecD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">secD</name>
        <name evidence="10" type="ordered locus">THA_199</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a complex with SecF. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579407.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_199"/>
    <dbReference type="EnsemblBacteria" id="ACJ74704">
        <property type="protein sequence ID" value="ACJ74704"/>
        <property type="gene ID" value="THA_199"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_199"/>
    <dbReference type="eggNOG" id="COG0342">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007894_4_2_0"/>
    <dbReference type="OMA" id="MVVYYRL"/>
    <dbReference type="OrthoDB" id="121331at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01463_B">
        <property type="entry name" value="SecD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005791">
        <property type="entry name" value="SecD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022645">
        <property type="entry name" value="SecD/SecF_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00916">
        <property type="entry name" value="2A0604s01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01129">
        <property type="entry name" value="secD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="434"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="477" mass="51837" checksum="E937502E82F97733" modified="2009-02-10" version="1">MKDNKVRGIITLIVIALAFLSLLWPTSEGYKGLASLFSRIKLGLDISGGARIEYKVDIDKSVENPSQVAEDVWTVLRNRLDMANYTEAIVKQTFREENTFIIVEIPGATDTARAEQLIGSTGLLWFGQVIDSTTSDPNIDPNLVNEAKREKAEWLLDREGKTWYLVKKEIANISNLKLVSPKIVEAIPQVDRNNVAGYVVTFKLDKQYADVFKRITEKLYVPEDLINQGGVAYQQALKKRLAIVLDDRVQFAGFVTAKITDGSALIKGNFTLDEAKQLAAILKSGALPARLEKVSSGWVAPLLGADIVSASLKAGIIGIILVLIYMIIFYGVMGIVADIALLYNTFLLLGILAATGSILTLPGIAGIILTIGTTVDGNIIIYERIKEEIRRGSSVKAAISTAFSKSFITLFDANLTTIIAGLFLYYFGTGTVKGFAITLIIGVLGSLFVNLIVSRFFLELFSGAIKVKGASKGGARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF48</accession>
    <name>B7IF48_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NAD-dependent deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_207</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579414.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_207"/>
    <dbReference type="EnsemblBacteria" id="ACJ74712">
        <property type="protein sequence ID" value="ACJ74712"/>
        <property type="gene ID" value="THA_207"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_207"/>
    <dbReference type="eggNOG" id="COG0846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023643_3_0_0"/>
    <dbReference type="OMA" id="ERDCHGL"/>
    <dbReference type="OrthoDB" id="1264438at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="235" mass="26778" checksum="D5DE4B29C2E4D66B" modified="2009-02-10" version="1">MLKELIRIIKNGNIVVLTGAGISTKSGIPDFRSKDGLYQKYGEKIFDLQFFFENPSVFYDFVYKEFPKMYDAKPNFAHIFLAKLEKNGYINGIITQNIDNLHYKAGSKNVLELHGNATRFYCTKCGKQSKNTFDGYICECGGLIRPDIVFFSESVRYLEESYTLIDNSSTLIVVGSSLQVYPAAYLPIYAKKQNKTLAIINKGKTPLDDYADIIIYDDIVETFEKIAKHFEEVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF52</accession>
    <name>B7IF52_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">DNA polymerase I</fullName>
            <ecNumber evidence="2 11">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">polA</name>
        <name evidence="16" type="ordered locus">THA_211</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the DNA polymerase type-A family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2 11"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579418.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_211"/>
    <dbReference type="EnsemblBacteria" id="ACJ74716">
        <property type="protein sequence ID" value="ACJ74716"/>
        <property type="gene ID" value="THA_211"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_211"/>
    <dbReference type="eggNOG" id="COG0258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0749">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004675_0_0_0"/>
    <dbReference type="OMA" id="NRPPMPD"/>
    <dbReference type="OrthoDB" id="1220182at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09898">
        <property type="entry name" value="H3TH_53EXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020046">
        <property type="entry name" value="5-3_exonucl_a-hlix_arch_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002421">
        <property type="entry name" value="5-3_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019760">
        <property type="entry name" value="DNA-dir_DNA_pol_A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020045">
        <property type="entry name" value="DNA_polI_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018320">
        <property type="entry name" value="DNA_polymerase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="PTHR10133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01367">
        <property type="entry name" value="5_3_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02739">
        <property type="entry name" value="5_3_exonuc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00475">
        <property type="entry name" value="53EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="SSF47807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00593">
        <property type="entry name" value="pola"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00447">
        <property type="entry name" value="DNA_POLYMERASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="53EXOc" evidence="14">
        <location>
            <begin position="2"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="3'-5' exonuclease" evidence="13">
        <location>
            <begin position="298"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="POLAc" evidence="15">
        <location>
            <begin position="652"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="627"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="627"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ACJ74716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="892" mass="103124" checksum="98A9F17857CF28C3" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF53</accession>
    <name>B7IF53_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxymuconolactone decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579419.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_212"/>
    <dbReference type="EnsemblBacteria" id="ACJ74717">
        <property type="protein sequence ID" value="ACJ74717"/>
        <property type="gene ID" value="THA_212"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_212"/>
    <dbReference type="eggNOG" id="COG0599">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137228_2_3_0"/>
    <dbReference type="OMA" id="LRCDDCV"/>
    <dbReference type="OrthoDB" id="1925754at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="26"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="121" mass="13962" checksum="3A760545B3D99C9A" modified="2009-02-10" version="1">MNLEEFKKFREKMNERILEEGTLNTKRFFNLDGNVYKNGALDEKTKELMGLVASMVLRCDDCITYHMIRCAQLGVSDEEFFETFDVALIVGGSIVIPHLRRAVKLLEDIREMQKNGKDVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF96</accession>
    <name>B7IF96_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="7">DMRL synthase</shortName>
            <shortName evidence="7">LS</shortName>
            <shortName evidence="7">Lumazine synthase</shortName>
            <ecNumber evidence="4 7">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">ribH</name>
        <name evidence="8" type="ordered locus">THA_259</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="4 7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579450.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_259"/>
    <dbReference type="EnsemblBacteria" id="ACJ74760">
        <property type="protein sequence ID" value="ACJ74760"/>
        <property type="gene ID" value="THA_259"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_259"/>
    <dbReference type="eggNOG" id="COG0054">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089358_1_1_0"/>
    <dbReference type="OMA" id="CQGVTQG"/>
    <dbReference type="OrthoDB" id="1680292at2"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09209">
        <property type="entry name" value="Lumazine_synthase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="PTHR21058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="54"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="77"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="1-deoxy-L-glycero-tetrulose 4-phosphate binding" evidence="7">
        <location>
            <begin position="82"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="156" mass="17056" checksum="DD715C6AB1445BC7" modified="2009-02-10" version="1">MILEGQYQNCEDLKIGIVVSRFNSQITEMLLNGALDCLKRHGVKNTEIIKVPGSMEIGFVLKKLVNLDYDALIALGAIIRGETYHFEVVSNEISKAVMQLNLEGKVPISFGVVTADTLEQAINRAGAKMGNKGFEAAMVALEMANINRKINSIKST</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFB5</accession>
    <name>RS15_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsO</name>
        <name type="ordered locus">THA_279</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103868.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFB5"/>
    <dbReference type="SMR" id="B7IFB5"/>
    <dbReference type="STRING" id="484019.THA_279"/>
    <dbReference type="EnsemblBacteria" id="ACJ74779">
        <property type="protein sequence ID" value="ACJ74779"/>
        <property type="gene ID" value="THA_279"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_279"/>
    <dbReference type="eggNOG" id="COG0184">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148518_0_0_0"/>
    <dbReference type="OMA" id="FKTHVKD"/>
    <dbReference type="OrthoDB" id="1990141at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_B">
        <property type="entry name" value="Ribosomal_S15_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_S15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_S15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="S15_NS1_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321">
        <property type="entry name" value="PTHR23321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="SSF47060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00952">
        <property type="entry name" value="S15_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000143182" description="30S ribosomal protein S15">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <sequence length="84" mass="10003" checksum="5E97E8955D815757" modified="2009-02-10" version="1">MNKEEIIKEFQIHEGDTGSTEVQIALLTARIKHLTEHLKKHPKDYHSRRGLMKLVGRRRKILKYLRNKDPESYKNVIQKLGLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFE8</accession>
    <name>B7IFE8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cmr crispr-associated protein, Cmr3 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_312</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579491.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_312"/>
    <dbReference type="EnsemblBacteria" id="ACJ74812">
        <property type="protein sequence ID" value="ACJ74812"/>
        <property type="gene ID" value="THA_312"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_312"/>
    <dbReference type="eggNOG" id="COG1769">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044328_1_0_0"/>
    <dbReference type="OMA" id="IYFEPED"/>
    <dbReference type="OrthoDB" id="1656967at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010165">
        <property type="entry name" value="CRISPR-assoc_prot_TM1793"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019117">
        <property type="entry name" value="CRISPR-assoc_protein_Cmr3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09700">
        <property type="entry name" value="Cas_Cmr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01888">
        <property type="entry name" value="cas_cmr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="350" mass="41011" checksum="79722C2B3E5A9941" modified="2009-02-10" version="1">MSKVKVLKFIPIDWVSFRKSKNFSTFENTIFPNIKTFYGAIFSAYFRKRGTKNAIKEIEKMIEEKLINLIGPFIVSERNEIYFRKPANLKLRKICKNENSCEYEYEYYKGFPCQDSFAVDDKNLRYIKYSNIDNLSEEGITFISISDLETFKKSSDKKLYLNLHKYNKNLPFSIERKVGIALDNKKRMVDKEHGAFYLLSTYRFRDGAGFGVFIDEKTEQFLNENKINTVNIGSKGKLARLEIFEIETDLFSKESSSKIKGLMLLTPAVFKNGFLPTSEYIRQKIVAVCNYKPEVYSGWDLKKNKPGKMFRIVPPGSVFFTEEKYLENLELTEEFNEYNYGKFIVLENCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFF4</accession>
    <name>B7IFF4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_318</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579497.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_318"/>
    <dbReference type="EnsemblBacteria" id="ACJ74818">
        <property type="protein sequence ID" value="ACJ74818"/>
        <property type="gene ID" value="THA_318"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_318"/>
    <dbReference type="eggNOG" id="ENOG50347DG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1085589_0_0_0"/>
    <dbReference type="OMA" id="VTPINFY"/>
    <dbReference type="OrthoDB" id="1777594at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="254" mass="29142" checksum="1D6ADCC0D757079C" modified="2009-02-10" version="1">MSFLKKEFELYKNLFGKYKLNIESLKIVPEGKALEAGDVFVIYGDILVYGVVIEKNGDLAECVLLSQDVFLSSSKALKIKINHLVDYVYVTHVNFYIEDNFAKNYCQVIINVKEDINKIAENVEELEEQIYTGPRKEYFDLLIDQISLLYDIFFEKIFDETKEEDKIIEINIPYASEFALVAGTNSLRGENFVGIVENNTLKVYLQDNAIGKKGKLIFKNQVIYEGELPNAFVSNNIKVSVEDLKKFLVIEVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFJ1</accession>
    <name>B7IFJ1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579521.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_361"/>
    <dbReference type="EnsemblBacteria" id="ACJ74855">
        <property type="protein sequence ID" value="ACJ74855"/>
        <property type="gene ID" value="THA_361"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_361"/>
    <dbReference type="eggNOG" id="ENOG5032T73">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1309247_0_0_0"/>
    <dbReference type="OMA" id="HVWWISP"/>
    <dbReference type="OrthoDB" id="1914620at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="203" mass="24226" checksum="06C6066E9C4B7CDC" modified="2009-02-10" version="1">MIHLFLGHYVADHGFTHNSKLRHLKGWKLIEHLIWSVFAILAFTFDTLLKDAPVILFTFIAIHLVLDFLRTKVKKESFYHLIEISGIILALIFNYSVYKYFYTSYLSREFVLYLLGMALVTTALSYFFRNFYPKDEMFEDLEGISERLAFFVFFLANKPFFAFLSLLLGFMYRLWKVKKFDHVWWISPAFAVVFSMIWKVIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFK0</accession>
    <name>B7IFK0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104067.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_370"/>
    <dbReference type="EnsemblBacteria" id="ACJ74864">
        <property type="protein sequence ID" value="ACJ74864"/>
        <property type="gene ID" value="THA_370"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_370"/>
    <dbReference type="eggNOG" id="COG1937">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130332_2_2_0"/>
    <dbReference type="OMA" id="YCIDVAN"/>
    <dbReference type="OrthoDB" id="1868412at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003735">
        <property type="entry name" value="Metal_Tscrpt_repr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038390">
        <property type="entry name" value="Metal_Tscrpt_repr_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33677">
        <property type="entry name" value="PTHR33677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02583">
        <property type="entry name" value="Trns_repr_metal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="90" mass="10224" checksum="6C38FC2D39429344" modified="2009-02-10" version="1">MKHKDALRTLKNARGQIDAIIKMIEENRYCIDISKQILAAISLLKKANVQVLNSHLETCVKSAAFSDDPQEVEQKIKELEDVISYLNKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFT5</accession>
    <name>B7IFT5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tail-specific protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_458</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the peptidase S41A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579595.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_458"/>
    <dbReference type="MEROPS" id="S41.004"/>
    <dbReference type="EnsemblBacteria" id="ACJ74949">
        <property type="protein sequence ID" value="ACJ74949"/>
        <property type="gene ID" value="THA_458"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_458"/>
    <dbReference type="eggNOG" id="COG0793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017295_3_0_0"/>
    <dbReference type="OMA" id="LANDIHY"/>
    <dbReference type="OrthoDB" id="1646508at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07560">
        <property type="entry name" value="Peptidase_S41_CPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004447">
        <property type="entry name" value="Peptidase_S41A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005151">
        <property type="entry name" value="Tail-specific_protease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03572">
        <property type="entry name" value="Peptidase_S41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00245">
        <property type="entry name" value="TSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00225">
        <property type="entry name" value="prc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="94"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="403" mass="44668" checksum="C3B281BD46C8FA5A" modified="2009-02-10" version="1">MKKKNFIVITAVIISILVGTIILSGATTDKNFQDNLTPLAETLYYILNYYYEIDKADINKVIDYGIDGLVKGLGDDFSYYYNKDVYEEKEIENKGEYGGLGIEVTYDADSKAIKIISPMYGTPAWRAGLKAGDLIISVDGSSVQEMSYIEAVNRMRGEPGTKVKLTILRGEEVLEFEITREIIKITPVKYGFVETQIGRIGYVRLTQFNQPSSKKLEEALNKIYEKGVVALIFDLRDNPGGYLDSAIDVASMFLDAGKLVVTVEPRVGSIERYVSKGNDFPKVPITVLVNGGSASASEIVTGALKENNRAVVIGQKTFGKGSVQQGFPLSNGGVLFITIAHYKTPSGNDIHRVGIEPNIYVTQEATDNVAHEEEVVDYTKEITEVNLDDPYIKRAIQYFIEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFT9</accession>
    <name>B7IFT9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104242.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_462"/>
    <dbReference type="EnsemblBacteria" id="ACJ74953">
        <property type="protein sequence ID" value="ACJ74953"/>
        <property type="gene ID" value="THA_462"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_462"/>
    <dbReference type="eggNOG" id="COG1713">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089580_1_2_0"/>
    <dbReference type="OMA" id="ILTRNWA"/>
    <dbReference type="OrthoDB" id="1433958at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005249">
        <property type="entry name" value="YqeK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00488">
        <property type="entry name" value="TIGR00488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="22"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="194" mass="22641" checksum="E97FA3C3F750B5E3" modified="2009-02-10" version="1">MIVDIDEIKNVVNLLVSKDRLLHVEGTAKFAKTLARVHGLDENIAEFIGYAHDIFRDINQRKLLKLARGYNLSLTDEEKLHPILLHGKLAAEFLRFRFNVDDEELLDAVRYHTSGYKNFGIYGKLLFLADSLEETRNYPNVNKLREIAFKDIEMGYFEVLRNKLIYAIERNLFILKESIESWNELIRKRKGGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFX9</accession>
    <name>B7IFX9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_504</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579619.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_504"/>
    <dbReference type="EnsemblBacteria" id="ACJ74993">
        <property type="protein sequence ID" value="ACJ74993"/>
        <property type="gene ID" value="THA_504"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_504"/>
    <dbReference type="eggNOG" id="COG2206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_594374_0_0_0"/>
    <dbReference type="OMA" id="EFCCEND"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="HD-GYP" evidence="2">
        <location>
            <begin position="273"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="460" mass="53386" checksum="8562DF89713CE1E7" modified="2009-02-10" version="1">MLFEKCIVIFLSVSLSGVSPIFGVFFLMFLIIYYILKNSYVRFAKELEDELKILKNSMDHFQNYLELSTVNKELRISELDELKERFNYLQKVVLDNEKELQEMFYSEERLRKEVEQLNNLLVSVYDILSNIVPEEDLEEIAHKILKKLIYIIPDVNAGSIILKDGGKYRFYAQMGYSDALKDIVFTDEDYVVAVKYEGFVDGKIFKEFNEKNVFKDKIEKLGSNKIKSSYIIPIYVNNEKLGGICLDSFVNEKLVIPEYATQIFGKLFGNFLYLKLYGKRMEKLFEEILTMLVEVEEKLSNKKHSKMLANVSKIVAEKMGLDVEKVYRAAYLHDIGKIMVDKTVLKKPGRLTEKERKEIESHVKHGYELLNKFEAFKDIAEIVLYHHERCDGKGYPKGLKCEQIPIESKIISAGDVCCTLLIKGYGKEEIINLLRKDVENGKLDGNIVEILIELLRNNII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFY1</accession>
    <name>ISPG_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)</fullName>
            <ecNumber evidence="1">1.17.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispG</name>
        <name type="ordered locus">THA_506</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + H2O + oxidized [flavodoxin] = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin]</text>
            <dbReference type="Rhea" id="RHEA:43604"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:10623"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:58483"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="EC" id="1.17.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IspG family.</text>
    </comment>
    <dbReference type="EC" id="1.17.7.3" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579621.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFY1"/>
    <dbReference type="SMR" id="B7IFY1"/>
    <dbReference type="STRING" id="484019.THA_506"/>
    <dbReference type="EnsemblBacteria" id="ACJ74995">
        <property type="protein sequence ID" value="ACJ74995"/>
        <property type="gene ID" value="THA_506"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_506"/>
    <dbReference type="eggNOG" id="COG0821">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042258_0_0_0"/>
    <dbReference type="OMA" id="PTCGRTQ"/>
    <dbReference type="OrthoDB" id="665306at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00096"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046429">
        <property type="term" value="F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00159">
        <property type="entry name" value="IspG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016425">
        <property type="entry name" value="IspG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004588">
        <property type="entry name" value="IspG_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30454">
        <property type="entry name" value="PTHR30454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04551">
        <property type="entry name" value="GcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004640">
        <property type="entry name" value="IspG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00612">
        <property type="entry name" value="ispG_gcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000191088" description="4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)">
        <location>
            <begin position="1"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00159"/>
        </source>
    </evidence>
    <sequence length="344" mass="37815" checksum="3AD21B83A3711A4E" modified="2009-02-10" version="1">MTKEINIKNVKIGGKNSIVIQSMTNTKTEDIKSTLQQIQNLYNAGCELVRVSAPTFEAAKALKTITKESPIPVIADIHFDYKLAIESIKNGASKVRINPGNIGNDEKVKEIIKVAKEYNVPIRVGANSGSIPKELETLPKIDALCESALKEVRLLEKFDFENIVISVKSSDVIETIKAYEKISKMTDYPLHIGVTEAGTYEWAIIKSSAALGYLLYKGIGDTIRISIAGDPVKEVLAAKKLLISLNLRKGIQIIACPTCARTTIDVEKWASIIEKRFSKVEKNIKIAVLGCVVNGIGEGKDAEIGIAGVQNGFLLFYKGKIVGTFKKEEIFQVLEDYIIKVEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG06</accession>
    <name>B7IG06_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MTS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579635.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_532"/>
    <dbReference type="EnsemblBacteria" id="ACJ75020">
        <property type="protein sequence ID" value="ACJ75020"/>
        <property type="gene ID" value="THA_532"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_532"/>
    <dbReference type="eggNOG" id="COG4123">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061983_3_1_0"/>
    <dbReference type="OMA" id="THFPTHA"/>
    <dbReference type="OrthoDB" id="1027015at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044260">
        <property type="term" value="P:cellular macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043412">
        <property type="term" value="P:macromolecule modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MTS" evidence="1">
        <location>
            <begin position="32"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="216" mass="24701" checksum="B5E409E0B36576EA" modified="2009-02-10" version="1">MIKLPPFDKNLGRDIKTIDIDGHKPTHASVFLLWYSLPTRDIKNVCELGSGTGFVSFGLSKYYNLKVTGIEVQEELFNASVKAIELNNVYNVDFFNLDLKDVKNHFSAESFDMVVFNPPYHFSSSSENKIREISRKSSQELLEIFISSASFLLRNRGTFVTVVAPYIFPLLNNILLKYRLTPQQMCIAYGKKAELIAIRGRKNGGMHFEIDRPVYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG39</accession>
    <name>B7IG39_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP synthase subunit c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP synthase F(0) sector subunit c</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">F-type ATPase subunit c</fullName>
            <shortName evidence="13">F-ATPase subunit c</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Lipid-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">atpE</name>
        <name evidence="15" type="ordered locus">THA_566</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Key component of the F(0) channel; it plays a direct role in translocation across the membrane. A homomeric c-ring of between 10-14 subunits forms the central stalk rotor element with the F(1) delta and epsilon subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell inner membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ATPase C chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100570.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_566"/>
    <dbReference type="EnsemblBacteria" id="ACJ75053">
        <property type="protein sequence ID" value="ACJ75053"/>
        <property type="gene ID" value="THA_566"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_566"/>
    <dbReference type="eggNOG" id="COG0636">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148047_2_1_0"/>
    <dbReference type="OMA" id="ALIFRQY"/>
    <dbReference type="OrthoDB" id="2090616at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005953">
        <property type="entry name" value="ATP_synth_csu_bac/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020537">
        <property type="entry name" value="ATP_synth_F0_csu_DDCD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031">
        <property type="entry name" value="PTHR10031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="SSF81333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01260">
        <property type="entry name" value="ATP_synt_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00605">
        <property type="entry name" value="ATPASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="13" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 13" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 13" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_C" evidence="14">
        <location>
            <begin position="16"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="site" description="Reversibly protonated during proton transport" evidence="13">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ACJ75053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="83" mass="8385" checksum="1779DE6DE075B4D4" modified="2009-02-10" version="1">MEKSLADALIIMGKAIGAGLAMGIGAIGPGIGEGNIGAHAMDAMARQPEMVGTITTRMLLADAVAESTGIYSLLIAFLILLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG80</accession>
    <name>B7IG80_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_608</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_608"/>
    <dbReference type="EnsemblBacteria" id="ACJ75094">
        <property type="protein sequence ID" value="ACJ75094"/>
        <property type="gene ID" value="THA_608"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_608"/>
    <dbReference type="HOGENOM" id="CLU_3349759_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="37" mass="4399" checksum="664FC7E7ACE41288" modified="2009-02-10" version="1">MNKLKDFRNGKTEVDENTKEIIEKVTGYKYESLYKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG95</accession>
    <name>B7IG95_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ggdef domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_623</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579691.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_623"/>
    <dbReference type="EnsemblBacteria" id="ACJ75109">
        <property type="protein sequence ID" value="ACJ75109"/>
        <property type="gene ID" value="THA_623"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_623"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038242_0_0_0"/>
    <dbReference type="OMA" id="NELTIHY"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="369"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="483" mass="55540" checksum="977F6FAE1230F91B" modified="2009-02-10" version="1">MKKLVFSILIFILLFLFLNQIVKVDGIPLNDINLPYTTLSKPSTITLSTTIKVDKLYSYLFFGKVSASKIKILVENDEIFELGTVSGNLWPTAIVVKIPEKFYNKKVNLKVVLFGIYDLGIHYTPVLTDQKAANLHATLIKVFRQDFYIFSQGITFIVGIILILSSLKFEKNIEKSIFYTGLSSIFTFLMLFDFQFRAYSGDISTFIFFRKISISSAVLALCFNVIGFSSLLNKNKKIFLLPTLILLSIFFAFNSLSSFNLLISILNVYIIILSSSIFYLVYKNKLKIFYFSTNFLILSILQTIYLLFTKASSEIMLPYGIVIFRTGISLILIKKFEEIVKEKENLKDLTFVDPMTQLFNRNIIENIPNKGKLILIDLDNFKELNDTLGHQFGDKILKEFADILKENTRSTDYVIRLGGDEFAIVTDTENPEQLVNRIRNISIKKLQLDFSYGISNNFNFDKAYSVADKRLYTMKNEKKKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGF2</accession>
    <name>B7IGF2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ion-translocating oxidoreductase complex subunit A</fullName>
            <ecNumber evidence="8">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Rnf electron transport complex subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnfA</name>
        <name evidence="9" type="ordered locus">THA_695</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NqrDE/RnfAE family.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104576.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_695"/>
    <dbReference type="EnsemblBacteria" id="ACJ75166">
        <property type="protein sequence ID" value="ACJ75166"/>
        <property type="gene ID" value="THA_695"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_695"/>
    <dbReference type="eggNOG" id="COG2209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095255_0_0_0"/>
    <dbReference type="OMA" id="YFLGMCS"/>
    <dbReference type="OrthoDB" id="1814639at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00459">
        <property type="entry name" value="RsxA_RnfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011293">
        <property type="entry name" value="Ion_transpt_RnfA/RsxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003667">
        <property type="entry name" value="NqrDE/RnfAE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02508">
        <property type="entry name" value="Rnf-Nqr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006102">
        <property type="entry name" value="NQR_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="197" mass="21604" checksum="A6569DC12F4311CC" modified="2009-02-10" version="1">MNPNINPLVLFFASIFTSNILLSNFLGMCSFISISKSLKSSNGLGMAVTMVMTITTAINWLVEKYIIVPFQIEYLRYIIYIIVIAAVVQILEMVIDRTAPNLYISLGIFLPLITVNCAILGVALFMQLRNYTFLQSIFFGLGSGLGWWLAIVLLAAIRIKVDKAPVPAPLKGVGITLITIGIMAMAFMGFAGMINVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGI2</accession>
    <name>B7IGI2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_728</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579756.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_728"/>
    <dbReference type="EnsemblBacteria" id="ACJ75196">
        <property type="protein sequence ID" value="ACJ75196"/>
        <property type="gene ID" value="THA_728"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_728"/>
    <dbReference type="eggNOG" id="COG1108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028808_3_0_0"/>
    <dbReference type="OMA" id="RIDLMAY"/>
    <dbReference type="OrthoDB" id="1421296at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="PTHR30477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="265" mass="29256" checksum="FE0EFED7A8BD2CE8" modified="2009-02-10" version="1">MEIFEFDFLKIAFLATILASISSALISPIVVYKRMEFIGDGTAHAAFAGLAFGLLFGINYRLMAVLTAIVFSIIISYFTNKSKIHENSAIGMLLPVFMSVGVILLSKSSTYVQDITSYLFGDILLVNISDFYFLMFIITLIIVFLAIFREEILYFLADEKMASFYGVKTSLLRALLLGVISVMVVGIVKISGVILLGALLIIPGLVSKSFAKSYKSVFFISVVYNVLVSIIGFYIAYILDISPGPSIVLTSFTAFVFLLFFKKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGK6</accession>
    <name>B7IGK6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_754</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579779.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_754"/>
    <dbReference type="EnsemblBacteria" id="ACJ75220">
        <property type="protein sequence ID" value="ACJ75220"/>
        <property type="gene ID" value="THA_754"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_754"/>
    <dbReference type="eggNOG" id="ENOG50332TV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_811172_0_0_0"/>
    <dbReference type="OMA" id="ATIFECE"/>
    <dbReference type="OrthoDB" id="8672at200918"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="300" mass="32599" checksum="214B955A7D89AAD4" modified="2009-02-10" version="1">MNEEIRKVLEALKNGEITTEEAEALIEAIKQREDYKDENFESSDENVETKVGEITVIEEGEVFEGDINIVNGEAVIKGIVNGDCSIVMGKLTFSGEVKGDMNIVGSRVKWNGGVIDGSLNIVASKEEGTPPKVKGGLTRVNNLLLKGLFKVFLKPFLSGFEIKNGNFKKSKKTQKFETLIVEEGKEFKTDDDIVAEEVIVKGKLACGNLKADKIRAIGTISCGNIETEELIVEGTVKTGNVKAENITVYQDGSISSGNVHAENIELNGVISSGNVTCEVIWGNGKLNAGWLNCEENRLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGL1</accession>
    <name>B7IGL1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polysaccharide deacetylase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579783.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_760"/>
    <dbReference type="EnsemblBacteria" id="ACJ75225">
        <property type="protein sequence ID" value="ACJ75225"/>
        <property type="gene ID" value="THA_760"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_760"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030024_0_0_0"/>
    <dbReference type="OMA" id="TYWHPNF"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="1">
        <location>
            <begin position="74"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="325" mass="38780" checksum="5D5E7DA1F38917B7" modified="2009-02-10" version="1">MKKIVIFLLLFSFISFSNIVIFIYHRFDDERYPTTNTWTSELEMHINLVKKLGYKIWTLKDLEDYVYGNKEEENAVIFTVDDGYVTTYTKAFPVFKKYNVPFSVFLYFGGVGVSKEYLTWDMIREMAEWGVEFGHHSTTHEKFPLLLEKMDLESFKKYFEDDLVKGQKIWQEKMNTSLKYYAYPYGYYNEEMIEILKKYGFRLAFIQLSGPYNKQISPFEIPREALLEDWATESHVRYVLSRQPLILKEKPYFWKDGKLYVKAKLDLSYSNPVVYIREKGIVESTFKDGYLQAGPFEINNDINSLMISARGKDRKEYVRYYLIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGM3</accession>
    <name>B7IGM3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_774</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 130 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100329.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_774"/>
    <dbReference type="EnsemblBacteria" id="ACJ75237">
        <property type="protein sequence ID" value="ACJ75237"/>
        <property type="gene ID" value="THA_774"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_774"/>
    <dbReference type="eggNOG" id="COG2152">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046648_0_0_0"/>
    <dbReference type="OMA" id="KAVQMNI"/>
    <dbReference type="OrthoDB" id="1622507at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007184">
        <property type="entry name" value="Mannoside_phosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34106">
        <property type="entry name" value="PTHR34106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04041">
        <property type="entry name" value="Glyco_hydro_130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016202">
        <property type="entry name" value="PH1107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="324" mass="37371" checksum="C139C27DDD74E081" modified="2009-02-10" version="1">MKIFAGKLSNIPWQERPKESSELIWRYSNNPIIKRDQAKDANSIFNSAVVPFKDTFAGVFRVDDRARNMNIRRGFSNDGINWKIDDKPIEFIQETRDELKSEYKYDPRVTFIEDRYWITWCNGYNGPTIGVGYTFDFEKFYQMENAFLPFNRNGVLFPRKINGKYAMLSRPSDNGHTPFGDIFYSESPDMIHWGVHRLVMKSGYTPWQSLKIGAGPSPIETDEGWLLFYHGVLLSCNGYVYSFGAALLDLDKPWKVIKRSKSYLLSPQKPYECVGDVPNVVFPVACLVDSETGRLALYYGAADTVVSLAFGYLHEIIDWLKYEN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGP0</accession>
    <name>DDL_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">D-alanine--D-alanine ligase</fullName>
            <ecNumber evidence="2">6.3.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">D-Ala-D-Ala ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">D-alanylalanine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ddl</name>
        <name type="ordered locus">THA_792</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + 2 D-alanine = ADP + D-alanyl-D-alanine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11224"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
            <dbReference type="ChEBI" id="CHEBI:57822"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the D-alanine--D-alanine ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.4" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579799.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IGP0"/>
    <dbReference type="SMR" id="B7IGP0"/>
    <dbReference type="STRING" id="484019.THA_792"/>
    <dbReference type="EnsemblBacteria" id="ACJ75254">
        <property type="protein sequence ID" value="ACJ75254"/>
        <property type="gene ID" value="THA_792"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_792"/>
    <dbReference type="eggNOG" id="COG1181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039268_0_2_0"/>
    <dbReference type="OMA" id="NTTPGMT"/>
    <dbReference type="OrthoDB" id="764798at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008716">
        <property type="term" value="F:D-alanine-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00047">
        <property type="entry name" value="Dala_Dala_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000291">
        <property type="entry name" value="D-Ala_lig_Van_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005905">
        <property type="entry name" value="D_ala_D_ala"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011095">
        <property type="entry name" value="Dala_Dala_lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011127">
        <property type="entry name" value="Dala_Dala_lig_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07478">
        <property type="entry name" value="Dala_Dala_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01820">
        <property type="entry name" value="Dala_Dala_lig_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039102">
        <property type="entry name" value="Ddl/VanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01205">
        <property type="entry name" value="D_ala_D_alaTIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00843">
        <property type="entry name" value="DALA_DALA_LIGASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00844">
        <property type="entry name" value="DALA_DALA_LIGASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000189752" description="D-alanine--D-alanine ligase">
        <location>
            <begin position="1"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="139"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="169"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00047"/>
        </source>
    </evidence>
    <sequence length="367" mass="42037" checksum="67C2511613C437FD" modified="2009-02-10" version="1">MINVGLLFGSKSVEHEISIITAHQVLSFVDKNKYNIIPIYITKDGKWLTGKILEDLENFKNLERLEKKSKQISSISAKDGKLILHSNIKKITIDVCLLTFHGSNGEDGSIQGMLEFLNVPYTGCGMYSSMYTMDKVITKLILKEKNIPVVDFLYTNKKNYTNDFLNHCKEVLEYPMIVKPARLGSSIGVKKVNDKCELEEAIETAFSFDDKVIVEKWIDSRELNCAVMGYKNIVVSEIEEIKKQKDFFDYNEKYVQKGKKFSNHIIPAPIDENLKNTIKSIARDTFNALECHGNIRIDFLLSKDNKIYVNEVNSIPGALSYYLWQMSGFTFSQVIDNMISIAFEAFKDKKSKIYSIDTNLFDLKVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGW4</accession>
    <name>B7IGW4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Galactoside O-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_868</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104367.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_868"/>
    <dbReference type="EnsemblBacteria" id="ACJ75328">
        <property type="protein sequence ID" value="ACJ75328"/>
        <property type="gene ID" value="THA_868"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_868"/>
    <dbReference type="eggNOG" id="COG0110">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_8_1_0"/>
    <dbReference type="OMA" id="IDDFCVL"/>
    <dbReference type="OrthoDB" id="1878940at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="190" mass="20921" checksum="DCDF5DC74C8F8FD7" modified="2009-02-10" version="1">MKTSFYTPEELKELGFKSIGKNVLISRKASIYKPELIEIGDNVRIDDFCILSGKIKLGSFIHIAAGCYLFAGEAGIIMEDFSGLSSRVSVYAITDDYSGEFLTNPMVPEKYRNIISEPVIIKKHGLIGTGATILPGVTIGEGAAVGSMALVNKDIPEWTIAVGIPAKPVKERKRDLLELEKRLYEELKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGW7</accession>
    <name>B7IGW7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_871</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104359.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_871"/>
    <dbReference type="CAZy" id="GT56">
        <property type="family name" value="Glycosyltransferase Family 56"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75331">
        <property type="protein sequence ID" value="ACJ75331"/>
        <property type="gene ID" value="THA_871"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_871"/>
    <dbReference type="eggNOG" id="COG0554">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061161_0_0_0"/>
    <dbReference type="OMA" id="VIVPMGY"/>
    <dbReference type="OrthoDB" id="1834836at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008417">
        <property type="term" value="F:fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009246">
        <property type="term" value="P:enterobacterial common antigen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036065">
        <property type="term" value="P:fucosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009993">
        <property type="entry name" value="WecF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07429">
        <property type="entry name" value="Glyco_transf_56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="358" mass="42445" checksum="D27DE9FC2E5A77BE" modified="2009-02-10" version="1">MYLHILNDNKYSDKFIEFINQNFSISDHHFVTFSKRPKYLDRGKVEIVDIYKLSQIRWLYKKISNADKIFLHSLFRGGKSLMLFLFNRKNLYKTYWVVWGGDLYNYWLKDSHSVREKVLEKLKRKVIKKIYGIIALVQEDYLFAKEKYKTKAKYYYAFYLNPVDFKMLDTFSDQKNKEEKTILIGNSAAPTNNHFEILSSLSKYRLNNFKIICPLSYGSSQEYIKKVCEYGVKLFGDNFIALTEFLSPEEYAKILANVDVAIFAHRRQQALGNILALLYLGKKVYIRSDISSWAFFNRFGIKVFDTKNILDGSEKDIFTFEEKIAIKNREIVLNEFSEERCVQLWKNVFESKLNGRDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGX1</accession>
    <name>B7IGX1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_875</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579850.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_875"/>
    <dbReference type="EnsemblBacteria" id="ACJ75335">
        <property type="protein sequence ID" value="ACJ75335"/>
        <property type="gene ID" value="THA_875"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_875"/>
    <dbReference type="eggNOG" id="ENOG502ZK8Y">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195306_0_0_0"/>
    <dbReference type="OMA" id="IPREVYH"/>
    <dbReference type="OrthoDB" id="2000748at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="63" mass="7292" checksum="84CCE27BEBA032C1" modified="2009-02-10" version="1">MFWNIVRLFMELVERPGEGENKKKEVIELVDSILDDLNIDIPRDILEIILSPAIDYLSAIMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGX7</accession>
    <name>B7IGX7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase, group 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_881</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_881"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75341">
        <property type="protein sequence ID" value="ACJ75341"/>
        <property type="gene ID" value="THA_881"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_881"/>
    <dbReference type="eggNOG" id="COG0297">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_4_0"/>
    <dbReference type="OMA" id="PMMPRYP"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="85"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="202"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="384" mass="44159" checksum="12AB0729D8DD3050" modified="2009-02-10" version="1">MGSESSGIFIIKRLEYLSRFNIDYNFLPISTEDSLLISTIKKLFKQEPKKLVEVVVGDRVFNPQKIDLSFFNRIKILKKNYIAWMKYAKKMAKILEKEMLNKKYDLIHAHRGFPEGVAANIASKSQNVPYVVTVHGGEIHSLMRYFKDTIIRTLEEAKKVFFVSEFLLNFAKKKGYSGKNAVVIPNGYDPQIFFPIDKEVIRKKLGVYKEGYKYVGFVGNLIPVKRADKLAEIFKSIKKFVPNTKFIVVGDGFLKEQIKSETRKLDIIFTGRISQREVAEWMNVMDVMILPSRNEGWPCVIVEAQACGTCVIGSNNGGIPEAVGFEEYIVDEGKNFEERFANKVVEILVNGYIPDQFIKHAKKFTWENIVKMEIEEYKKLGITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGX9</accession>
    <name>B7IGX9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="4 7">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">THA_883</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H2O + 2 NAD(+) + UDP-alpha-D-glucose = 3 H(+) + 2 NADH + UDP-alpha-D-glucuronate</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="4 7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579858.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_883"/>
    <dbReference type="EnsemblBacteria" id="ACJ75343">
        <property type="protein sequence ID" value="ACJ75343"/>
        <property type="gene ID" value="THA_883"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_883"/>
    <dbReference type="eggNOG" id="COG1004">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_2_0_0"/>
    <dbReference type="OMA" id="IELANTH"/>
    <dbReference type="OrthoDB" id="647136at2"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028357">
        <property type="entry name" value="UDPglc_DH_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500134">
        <property type="entry name" value="UDPglc_DH_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDPG_MGDP_dh_C" evidence="11">
        <location>
            <begin position="302"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="142"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="242"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ75343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="390" mass="44522" checksum="28B873C538681C97" modified="2009-02-10" version="1">MKISVVGLGYVGLSIAVLISQNQEVIGLDISEDKVKKINNKISPIKDKEIIEYLKHKNLNLTATTDKELAYKNAEMIFIATPTDYNPETNYFDTSSVDEVIEDVLKVNEKALIVIKSTIPIGYTERKRREFNTDRIIFSPEFLREGKALYDNLYPSRIVVGDKTEKGEKIARLLLEGAIKKDVPVFLMDSTEAEAVKLFANTYLAMRVAFFNELDTFAEIKGLNTLEIIKGVCSDPRIGDFYNNPSFGYGGYCLPKDTKQILADYLGNGVPHILIRAIVESNTIRKKHIAEMIASKKPKLVGIYRLAMKKDSDNFRHSSILDVIDYLKEKGVNMIIYEPIIKEDNFKSIKVERDFEKFISVSDLIVANRLYEELKIVKEKVYTRDLFERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGZ2</accession>
    <name>B7IGZ2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DHH family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100757.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_897"/>
    <dbReference type="EnsemblBacteria" id="ACJ75356">
        <property type="protein sequence ID" value="ACJ75356"/>
        <property type="gene ID" value="THA_897"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_897"/>
    <dbReference type="eggNOG" id="COG0618">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_933625_0_0_0"/>
    <dbReference type="OMA" id="PYVIFDH"/>
    <dbReference type="OrthoDB" id="1342626at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="298" mass="34945" checksum="8CE01CB7775919BD" modified="2009-02-10" version="1">MEHISFKELLNLKGKKILHTTHVKSDCDGIASIYWGLSIFGGSYFIPPYELRSAAGLIEMLNLKNSTDSIDDFDIIFIYDTEKRQDLPFKINKPYVIFDHHHKRDGEFVENALFCYIEPSSANVINLYNLSIKENLPLNDEILFSFAVALYTDTAMFRTAREGEFFYFSKFLKNRRFEDILDVVYYKDIDKSSFLKTIKKAKFYNINGLNVCVLKFKDNDQFYGFVDGLFNILNLDVLIGILKEGIKIHVKKRYVQKIYHMLFVPIQKKLNIERVQGIWMNFFDYKILLKALEDYKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH00</accession>
    <name>B7IH00_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved protein, with A weak D-galactarate dehydratase/altronate hydrolase domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579874.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_905"/>
    <dbReference type="EnsemblBacteria" id="ACJ75364">
        <property type="protein sequence ID" value="ACJ75364"/>
        <property type="gene ID" value="THA_905"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_905"/>
    <dbReference type="eggNOG" id="COG1672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021114_0_0_0"/>
    <dbReference type="OMA" id="RIGIECE"/>
    <dbReference type="OrthoDB" id="191489at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018631">
        <property type="entry name" value="AAA-ATPase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012547">
        <property type="entry name" value="PDDEXK_9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09820">
        <property type="entry name" value="AAA-ATPase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08011">
        <property type="entry name" value="PDDEXK_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA-ATPase_like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="527" mass="61969" checksum="8EA325E04E7782C1" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH21</accession>
    <name>B7IH21_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH dehydrogenase subunit n</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_926</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_926"/>
    <dbReference type="EnsemblBacteria" id="ACJ75385">
        <property type="protein sequence ID" value="ACJ75385"/>
        <property type="gene ID" value="THA_926"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_926"/>
    <dbReference type="eggNOG" id="COG0651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_653700_0_0_0"/>
    <dbReference type="OMA" id="IVHPEAP"/>
    <dbReference type="OrthoDB" id="1319844at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="356"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="3">
        <location>
            <begin position="92"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="420" mass="47849" checksum="C11F1D33DA8C8177" modified="2009-02-10" version="1">MFVFTLYLIKKTRYTLFIIPLSSIYLLFQRFDIVLNGYGGVRLVMDSSSFYFILTNIVVTILVSFQLVKHGNENTYFFFSLLHPVLNLLFISHDLFNIYVLLETITLLITLLILSSEKFEHKLTALKYIFASSLAMSLYLIGVGMYYYVNGTFDILDVNGFPGFLIKAALFSKSGIFLFGMWLPEVHSNVEPEVSAMLSSIYTQSALFPLLRISGENEFFIVGTITFLFGVFFSIISSDLKKILAYSSMSQIGLMLLNPFFAPLYVLSHGISKAILFLTTRYQKRDLRIKREVNILLFLTMLICLLSLSGFSLTLGGYVKGKMIHGLFSYLISFLSSIYAGKILKLKIDFRFEKKFIYLFLASLILIFPYFNLKSFVVILGMLVGFLLNINLDLSFTTETILTLMTFSVSIFSLIGGIIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH43</accession>
    <name>B7IH43_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0111 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100886.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_948"/>
    <dbReference type="EnsemblBacteria" id="ACJ75407">
        <property type="protein sequence ID" value="ACJ75407"/>
        <property type="gene ID" value="THA_948"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_948"/>
    <dbReference type="eggNOG" id="COG1392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104916_1_1_0"/>
    <dbReference type="OMA" id="WVGDLAD"/>
    <dbReference type="OrthoDB" id="1837604at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002727">
        <property type="entry name" value="DUF47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018445">
        <property type="entry name" value="Put_Phosphate_transp_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36536">
        <property type="entry name" value="PTHR36536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01865">
        <property type="entry name" value="PhoU_div"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00153">
        <property type="entry name" value="TIGR00153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="13"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="212" mass="24676" checksum="E1E152A3D72F1D4A" modified="2009-02-10" version="1">MSLFFGKKEQVVIELFRQHLDAVENTIVKLQDLIYEEKKEKYKELAEEVRKAETTADQIRRKAELEMYSGAFLPNFRGDLLGTIEALDRIANKAESVADEIDLQDLTIPKEIHENLKALISKSLETYKALKEAAKEMFEDFEKASQMIVETERFEHETDEIERSTIRKIFSLDIPLAEKIQLKDLVHRIADISDTSEDVSDRIQIILYKRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH49</accession>
    <name>B7IH49_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Citrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">THA_954</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CitM (TC 2.A.11) transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579899.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_954"/>
    <dbReference type="EnsemblBacteria" id="ACJ75413">
        <property type="protein sequence ID" value="ACJ75413"/>
        <property type="gene ID" value="THA_954"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_954"/>
    <dbReference type="eggNOG" id="COG1055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011920_4_0_0"/>
    <dbReference type="OMA" id="SWGYYFK"/>
    <dbReference type="OrthoDB" id="626718at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015105">
        <property type="term" value="F:arsenite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000802">
        <property type="entry name" value="Arsenical_pump_ArsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00758">
        <property type="entry name" value="ARSENICPUMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="92"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="279"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="405"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="CitMHS" evidence="10">
        <location>
            <begin position="17"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="424" mass="46965" checksum="5E59A9A7C3E4E6A8" modified="2009-02-10" version="1">MEKIFAILIVAIAYGYIIFGKKYKAPIVFGLALIVAALKLVEGLEPENISRVVDFNTLGLLAGMTVIVEFLKKTGLFQFLAIRIVKKGGKRFFLTIVGLMALVALSSAFLDNLVTIILIAPMIFLITDSLGLNPIPFLMLTIIIDNIGGMSTLIGSPLNLVLGSVSGLGFNDFINNMWFITIISFLITVFMFKKYTKVDKSVEEKLEKLSDIDESRAITDKKSLKFTLIVFLIVLSLFGLHQVIELDLSFVALLGAIAVMLFHKKQFNDISNEIDWDTLFFYAGLYILSFTLEEIGITNLLAGLFSPLSNHQFLSIFVIFLFVSISIPWLSAVPGTLIIAPVIKILISSGFSSTFWWVYAISANLATNLTPLGAVQNIVGVNLLSKQIGRNFSFGEYIKWAFKPYLVTSLVGFLYILLRIYIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH61</accession>
    <name>B7IH61_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_966</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579907.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_966"/>
    <dbReference type="EnsemblBacteria" id="ACJ75425">
        <property type="protein sequence ID" value="ACJ75425"/>
        <property type="gene ID" value="THA_966"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_966"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_0"/>
    <dbReference type="OMA" id="QTCLEIM"/>
    <dbReference type="OrthoDB" id="1729688at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="122" mass="13623" checksum="466748D787F155E9" modified="2009-02-10" version="1">MKKILVIDDSEVLRKITSFNLKKAGFEVYEAVDGVDGLEKMKEIMPDLIILDIMMPRLDGFGVLKEKGQDDSIKDIPVIILTAKGGSEDERLAKSLGAKMVMTKPFSPSQLIEEVKRLIEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHA1</accession>
    <name>B7IHA1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-alpha-glucanotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579926.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1007"/>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75465">
        <property type="protein sequence ID" value="ACJ75465"/>
        <property type="gene ID" value="THA_1007"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1007"/>
    <dbReference type="eggNOG" id="COG0366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006462_2_4_0"/>
    <dbReference type="OMA" id="EGQTFWK"/>
    <dbReference type="OrthoDB" id="1573900at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015261">
        <property type="entry name" value="4-alpha-glucanotransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09178">
        <property type="entry name" value="DUF1945"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aamy" evidence="1">
        <location>
            <begin position="5"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="455" mass="53061" checksum="3BF3F642172D762E" modified="2009-02-10" version="1">MVGYEIYIRSFYDYNNDGTGDFKGLSNAVSYLKDLGVDLVWIMPHFKAPSYHGYDIIDFFDTTPSYGTLDDFKEMVEKLHEAGIKIAIDLPLNHVSSRHPWFKAAVSGDKKYEDFFLWADDGVDLNEKRPWDNEVIWHPYNGRWYYGVFGGSSPDLNYENEKVVEKALEIVEFWLNMGIDGFRFDAAKHIYDYDLQQKKFSYNHEKNIQFWKKVMDKARSIKSDVFAVTEVWDAPEIVAEYAKTIGCSFNFYFTEALRESINHGNTYKIWDCFSRTLTDDRGLYIPSNFSSNHDMTRLASSLQCEDQRKVFFAMLLTTPGIPFIFYGDELGMKGVYDPYFTESVIEPFPWYSSLSGDGQTLWKSVGFNHAFTGVSVEEQSQREDSLLNTVKRWIKFRKENEWMTNSWIVDLRTSEFVVGYTVTNGEKSLRIYHNISGHEEEFEGIRLKPFESKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHA6</accession>
    <name>B7IHA6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75470">
        <property type="protein sequence ID" value="ACJ75470"/>
        <property type="gene ID" value="THA_1012"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1012"/>
    <dbReference type="HOGENOM" id="CLU_3206350_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="45" mass="5426" checksum="07A3BBC992683F39" modified="2009-02-10" version="1">MRRLYAYLLNGRIKISNLPSRQTIHYRLKRLSKKEVSKKLKIAHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHC0</accession>
    <name>B7IHC0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CoA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579941.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1026"/>
    <dbReference type="EnsemblBacteria" id="ACJ75484">
        <property type="protein sequence ID" value="ACJ75484"/>
        <property type="gene ID" value="THA_1026"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1026"/>
    <dbReference type="eggNOG" id="COG1832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112567_1_2_0"/>
    <dbReference type="OMA" id="YVWFQPG"/>
    <dbReference type="OrthoDB" id="1910664at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA_binding" evidence="1">
        <location>
            <begin position="2"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="124" mass="14011" checksum="0F36C223D387F581" modified="2009-02-10" version="1">MDLTKIKKIALVGATTNREKFGNIILRDLTKKGFEIVPVTPRYDEIEGIKTVKSVKELSKDIDLIVFVVPPSIGIEVTKEVVSEGFKNLWYQPGAYSQEIDEYLKSVGIEAVHDICIMVETNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHD6</accession>
    <name>B7IHD6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cobyric acid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">cobQ</name>
        <name evidence="6" type="ordered locus">THA_1042</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579951.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1042"/>
    <dbReference type="EnsemblBacteria" id="ACJ75500">
        <property type="protein sequence ID" value="ACJ75500"/>
        <property type="gene ID" value="THA_1042"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1042"/>
    <dbReference type="eggNOG" id="COG1492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019250_2_2_0"/>
    <dbReference type="OMA" id="DVRMNPL"/>
    <dbReference type="OrthoDB" id="744477at2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01750">
        <property type="entry name" value="GATase1_CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00028">
        <property type="entry name" value="CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033949">
        <property type="entry name" value="CobQ_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004459">
        <property type="entry name" value="CobQ_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343:SF1">
        <property type="entry name" value="PTHR21343:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00313">
        <property type="entry name" value="cobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3 4" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GATase_3" evidence="5">
        <location>
            <begin position="243"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="467" mass="53112" checksum="7203D0CB512B2CF8" modified="2009-02-10" version="1">MVLMIQGTASNVGKTTIATALCRYFAKKGIDVVPFKAQNISLNSIVSDDGGEMAIAQYIQAIACNKKPSYIMNPILLKPDITGSQLIVKGKPVEKIKNKKYMYSSKEELLNIAYECLKNLEKKHELVIVEGSGSAAEINIKDISNMAIAKKANASVILVADIDRGGAFAQIAGTMVLFNKKERKLVKGYIFNKFKGDKRLLLDFPEKFGKRFNIKFLGTIEYFNHNLFEEDITPFKEGKGDLKVDILKLPHISNTFDFEPLFLNTNARYVENIREDVDLIIIPGTKSTIHDLLWLKEKNNQIKKALSKGAFLFGICGGYQMLGKKLVEEEKEYEGLGYLNSYTYFIGKKTVRNVFAKENLFNTTVKGFEIHHGITKSFEKPFSKIYFNKKVIKDGTIKDNIFATYIHNIFHNNDFLEKFLNFLRIQRGYSKKDIVVKTLEEEIDRVTEIITSSLNMKEIEKIVYSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHE2</accession>
    <name>B7IHE2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579957.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1049"/>
    <dbReference type="EnsemblBacteria" id="ACJ75506">
        <property type="protein sequence ID" value="ACJ75506"/>
        <property type="gene ID" value="THA_1049"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1049"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038242_0_0_0"/>
    <dbReference type="OMA" id="VHELNIT"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="374"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="490" mass="56341" checksum="B4093EC124F35623" modified="2009-02-10" version="1">MFRFYLKYFLIAFIIFVFFPTTFYYLSHPKGVKIVKAENGISLPISSTLNSRRTFHLKFQIPNGYSYVYFPYVDCSYIKVYSSGKLIGKYGFNDRNAHSWFVPMLFEIPDKINSLEVEISGVYSIGLDSFYLIKEGERKKYVFLRFLTDNLINISIGLVLTLGILLLMLSKNTNFLRKNAYVYFGLSSIFATVWLFDLLSFESFWFPLRKIFVSSAYFSLLLIIVGFEFYNFSKVSKIAKVVSILNLAAGVSGLLTFSDYQLKLVSTYASPLLIIDSLYLVYITFRAYIPLDMVFSSFFAITVFHDAVVMILNVPARFFSPYGVIAIYLSFVFNILFEYKEKSTEVNILYAQSIIDKLTGAYNRGVLNSNFLKKGDVLVFVDLNKFKQINDTLGHDVGDKVLQKLSSIIKSYISSSDLLVRMGGDEFLVVLKNKDESIAKDLMKKILEEFKKSFEFSPTFSWGISKIEDNNIDNAIRKADDLMYKMKRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHE5</accession>
    <name>B7IHE5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alpha-galactosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579960.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1053"/>
    <dbReference type="CAZy" id="GH36">
        <property type="family name" value="Glycoside Hydrolase Family 36"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75509">
        <property type="protein sequence ID" value="ACJ75509"/>
        <property type="gene ID" value="THA_1053"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1053"/>
    <dbReference type="eggNOG" id="COG3345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036399_0_0_0"/>
    <dbReference type="OMA" id="ILGCGAP"/>
    <dbReference type="OrthoDB" id="469334at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14791">
        <property type="entry name" value="GH36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002252">
        <property type="entry name" value="Glyco_hydro_36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="508" mass="58874" checksum="73024F28E9474958" modified="2009-02-10" version="1">MEIIKELKNGKIISKKIKGKPGKVKVFEEVLSDNYILINNWQSWGPTKVIPKDFKLNISKEFLEKARFSPNPNPELLLDYIVSDYFIATKDKLYGFLSSKIGHPYFLIKDGKIEGYIDYFEEEFEDYIETEPLAVLNGNIDILLREYGELLRDYNKPQFKDWNPVGWSSWYYYFENLTWDDVLKNLKLAKNYPFEVFQIDDSWQKDIGDWQPKESFPSLKEMAGTIRKFGFVPGIWLAPFSVSETSSIFKEHKDWLVKDDAGNPKVAYQNWGKNIYALDLSNPDVLDFIETLFKSLKDSGFEYFKIDFLFAGAIPGKRYQNISPIRAYRLGMEKIRKTVSDNFILGCGAPLLPSIGYVDGMRIGPDTAPYYDPKIPDNLGPNAYSALRNTITRYFMNKKLWLNDPDCLLLRKTETNLTTNQKKMFAITAGMLDNMILVSDDLELELDDFLIPTALKLKGGEYFVEGIMDKAFTIYTKNEKVGEVKFRICLETEKSEYYPDKDFLKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHF2</accession>
    <name>B7IHF2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Extracellular solute-binding protein, family 1, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579967.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1060"/>
    <dbReference type="EnsemblBacteria" id="ACJ75516">
        <property type="protein sequence ID" value="ACJ75516"/>
        <property type="gene ID" value="THA_1060"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1060"/>
    <dbReference type="eggNOG" id="COG2182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_10_1_0"/>
    <dbReference type="OMA" id="WYVDTRV"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="403" mass="45816" checksum="93BB03E3FABDB6E2" modified="2009-02-10" version="1">MKRILVVLLVVVALLSFSKTKLTFWGFMMNDEHAKKVLEKFMAENPDIEVEYVQLSWANGFDKIVTAIAGGEDLDVVELGNTWVANFAERNALESMDIFYKNYGKDYVGWNTVYYQGSYWAVPWLLGTRALFYNLELFEKAGLDPDNPPKTWEELYVAAKKINELSDDIYGFGMPAGENYSPWQQWFLPAVWGTGGDIITEDGKRALLTSCKVKETAEFYKALSEVSLKTKQADLAKAFGEGKIGLYVSGAWDIDYLETNYPETPFNVVFIPKPAAWYGTHASFAGAEVLAIMKHSKHKKEAQKLVEFLIRPDIAMEVAMIWPAIFPSHVDAGNDPWFEDHPMHKVFYEQNKYAKPVPSIPAWPKVQAVLTEAIEKIILQDADIDTTHFEYNMKIQKILDGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHI3</accession>
    <name>B7IHI3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">LemA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1091</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LemA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101187.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1091"/>
    <dbReference type="EnsemblBacteria" id="ACJ75547">
        <property type="protein sequence ID" value="ACJ75547"/>
        <property type="gene ID" value="THA_1091"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1091"/>
    <dbReference type="eggNOG" id="COG1704">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056714_2_1_0"/>
    <dbReference type="OMA" id="YNNAVQA"/>
    <dbReference type="OrthoDB" id="1573349at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023353">
        <property type="entry name" value="LemA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007156">
        <property type="entry name" value="MamQ_LemA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34478">
        <property type="entry name" value="PTHR34478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04011">
        <property type="entry name" value="LemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140478">
        <property type="entry name" value="SSF140478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="183" mass="21098" checksum="B3376A8BB1FBB80F" modified="2009-02-10" version="1">MIWVILGIILLILFWVIGTYNSLVKLKKMIENAWSQIDVQLKRRHDLIPNLVNSVKGYMKFEQETLEKVLQARASAISSKDISKKIEAENQLTGALSKLLAVVENYPDLKANENVSSLMEELRKTEDKISYARQFYNDIVMKYNTKISVFPSNIIASMFKFKEAPFFKVEEAEKEVPNVDLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHI6</accession>
    <name>B7IHI6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methionine synthase</fullName>
            <ecNumber evidence="6">2.1.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">5-methyltetrahydrofolate--homocysteine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ordered locus">THA_1094</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:11172"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="2.1.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetH route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the vitamin-B12 dependent methionine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.13" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579997.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1094"/>
    <dbReference type="EnsemblBacteria" id="ACJ75550">
        <property type="protein sequence ID" value="ACJ75550"/>
        <property type="gene ID" value="THA_1094"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1094"/>
    <dbReference type="eggNOG" id="COG0646">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004914_0_2_0"/>
    <dbReference type="OMA" id="ADCIAMS"/>
    <dbReference type="OrthoDB" id="118138at2"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00081"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008705">
        <property type="term" value="F:methionine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042558">
        <property type="term" value="P:pteridine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003759">
        <property type="entry name" value="Cbl-bd_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036594">
        <property type="entry name" value="Meth_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02607">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01018">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47644">
        <property type="entry name" value="SSF47644"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="SSF82282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51337">
        <property type="entry name" value="B12_BINDING_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="16" id="KW-0170">Cobalt</keyword>
    <keyword evidence="13 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="8 19 24" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11 19 24" id="KW-0808">Transferase</keyword>
    <keyword evidence="14 19" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Hcy-binding" evidence="20">
        <location>
            <begin position="3"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Pterin-binding" evidence="21">
        <location>
            <begin position="317"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="B12-binding N-terminal" evidence="23">
        <location>
            <begin position="560"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="B12-binding" evidence="22">
        <location>
            <begin position="653"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="ACJ75550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="773" mass="86231" checksum="E4E80EFC359E04F3" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHM0</accession>
    <name>B7IHM0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transporter, major facilitator family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580021.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1131"/>
    <dbReference type="EnsemblBacteria" id="ACJ75584">
        <property type="protein sequence ID" value="ACJ75584"/>
        <property type="gene ID" value="THA_1131"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1131"/>
    <dbReference type="eggNOG" id="COG2211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025379_2_1_0"/>
    <dbReference type="OMA" id="RAWMGSI"/>
    <dbReference type="OrthoDB" id="626204at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="450" mass="51455" checksum="5FCDCE76E274EBAD" modified="2009-02-10" version="1">MTKNESLLIVEGIFSNFYFILTQGVVFTSIALFFNFNEVLLGITAAFPMVFQLLQIVTPSIIEKFKRRKRLLMLFNSFKLLWFVVVISIMLGKKSPMILIITFAVSQAFTSLAGNTWSSLVSDIIPSEKRGRYFGIRSVLISFSTLLIFYLFSYIIDNVPEPYNYIYVISITLIGTILGLISLIPVDDPPLKTLGTISELKEVFSEKNFLKLAFANMYWNFILLLTAPFFSYHQLKNLHIPMTYISYATIVMTLISMLFYFIWGKMSDKLGNKTVMISGILIVSFTPFIWILMNEKYWPFAMGLDAVISGIGWAAINLSLLILPMEAAKRISPMYFAVFGFFGGLGGLIGSISGGYFAAIFNKYNFYINNYHIFGIQIYFVIEGILRLLAIPIFASVKTQKYVSPTIFVFNVLNVISRRPTQKIYENAKIESAYILKKVPLKKERVKRWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHN8</accession>
    <name>B7IHN8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glyoxylate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1150</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580038.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1150"/>
    <dbReference type="EnsemblBacteria" id="ACJ75602">
        <property type="protein sequence ID" value="ACJ75602"/>
        <property type="gene ID" value="THA_1150"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1150"/>
    <dbReference type="eggNOG" id="COG1052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_2_0"/>
    <dbReference type="OMA" id="KMKPNCI"/>
    <dbReference type="OrthoDB" id="1638924at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="4">
        <location>
            <begin position="3"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="5">
        <location>
            <begin position="108"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="317" mass="35978" checksum="F9383DFAA1433E69" modified="2009-02-10" version="1">MKVFITYKIPESGINLLKEKFDVDVYEGEKFLTKQEMMERLKDADAVVTQLRDPVDKEFIDAGKKLKIIANYAVGFNNIDVEYAKQKGIYVTNTPDVLTEATADIAWALILAVARKIIPADKFTREGKFEGWKPNLFLGYEIYGKTLGIIGMGRIGKAVARRAMGFGMKIIYHNRKRVEDDYKYNAKYVDLETLLKESDYISINAPLTNETYHLLNKERLSLLKKNAILVNTARGPIIDEKALYELLKDGKIAGAGFDVYENEPEITKGLEKLDNVVLLPHIGSATYETREKMSIMVAENIIDALEGKIPRNLVWKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHS3</accession>
    <name>B7IHS3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1187"/>
    <dbReference type="EnsemblBacteria" id="ACJ75637">
        <property type="protein sequence ID" value="ACJ75637"/>
        <property type="gene ID" value="THA_1187"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1187"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_676034_0_0_0"/>
    <dbReference type="OMA" id="ADDFAVW"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="396" mass="46511" checksum="D43DD42BF7CE59DA" modified="2009-02-10" version="1">MGDKVTKEASEIISSWMKQPVLTLLYEGPKEYKHELEEYFHRKGYLLGDGEKVYIFISKGEKIAWRVIYKDVDIDNFKSPSLDLTLLYQTLENLFEDQTLPDKSIAFDPITNQSYIVENQIYPFIIKHKKGSYKVEIEGKVYTFSEGFHTVEGQELYVGKDLKIEKKDNLLVGVFGVFGDYYYDGKQVIGKSFNIDFPEIPLYVDKGIVISRNFWYENGILQQNKIPVLDVFNGFVLYSDGSISDIHKLWRYEFDSVPFDWYYKDGIVSFAFLNGNVVLFDLTKRRKLFEGKYKKVLGVGFLKDALYIRTMDKIVKLDTRTGRILESYNENRDFILINDSIKFVDGIVLRPRYKNVWFDGIDYYFEDLKINGFKEVIAGNNYYYIILENGTWRIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHS4</accession>
    <name>B7IHS4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hemolysin A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TlyA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1188"/>
    <dbReference type="EnsemblBacteria" id="ACJ75638">
        <property type="protein sequence ID" value="ACJ75638"/>
        <property type="gene ID" value="THA_1188"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1188"/>
    <dbReference type="eggNOG" id="COG1189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058015_1_0_0"/>
    <dbReference type="OMA" id="VLMVKPQ"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004538">
        <property type="entry name" value="Haemolysin_A/TlyA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32319:SF0">
        <property type="entry name" value="PTHR32319:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005578">
        <property type="entry name" value="TlyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00478">
        <property type="entry name" value="tly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="2">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="260" mass="29499" checksum="B620D6A8DC575042" modified="2009-02-10" version="1">MKKERLDVLLVERGLIESREKAKRIIMSGNVFVNEQLVDKPGKLVDVESKIRIKETEKYVSRGGYKIEGAWESFKFKIEERVACDIGASTGGFTDFLLQHGAKKVYCVDVGYNQLHWKLRSDDRVVVMEKTNARNLTLPEKVDLAVCDVSFISLKKIFPTIKEILKEDGEAVVLVKPQFEAGKEKVGKGGIVRDKSVHKEVLYDIIDSAKEIGLSPINIDFSKITGTDGNIEYFLHLLNIKKEVSLEIDEVIDRAWEELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHS8</accession>
    <name>B7IHS8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative Chemotaxis protein CheY-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101411.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1192"/>
    <dbReference type="EnsemblBacteria" id="ACJ75642">
        <property type="protein sequence ID" value="ACJ75642"/>
        <property type="gene ID" value="THA_1192"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1192"/>
    <dbReference type="eggNOG" id="COG2201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_15_0"/>
    <dbReference type="OMA" id="AAGAHEY"/>
    <dbReference type="OrthoDB" id="1630092at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="120" mass="13072" checksum="C35787B6A382F234" modified="2009-02-10" version="1">MGKRILIVDDAAFMRMMLKDIVTKAGHEVVGEAANGKEAVEKYKELKPDIVTMDITMPEMNGIDAIKEIKKIDPNATIIVCSAMGQQAMVIEAIQAGAKDFIVKPFQPARVIEAIQKVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHT5</accession>
    <name>B7IHT5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">rpmI</name>
        <name evidence="7" type="ordered locus">THA_1199</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the bacterial ribosomal protein bL35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101418.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1199"/>
    <dbReference type="EnsemblBacteria" id="ACJ75649">
        <property type="protein sequence ID" value="ACJ75649"/>
        <property type="gene ID" value="THA_1199"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1199"/>
    <dbReference type="eggNOG" id="COG0291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169643_4_3_0"/>
    <dbReference type="OMA" id="RRHANAW"/>
    <dbReference type="OrthoDB" id="2046660at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00514">
        <property type="entry name" value="Ribosomal_L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021137">
        <property type="entry name" value="Ribosomal_L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001706">
        <property type="entry name" value="Ribosomal_L35_non-mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037229">
        <property type="entry name" value="Ribosomal_L35_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33343">
        <property type="entry name" value="PTHR33343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01632">
        <property type="entry name" value="Ribosomal_L35p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00064">
        <property type="entry name" value="RIBOSOMALL35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143034">
        <property type="entry name" value="SSF143034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00001">
        <property type="entry name" value="rpmI_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="67" mass="7982" checksum="ACB3F00814ECA0CD" modified="2009-02-10" version="1">MAKQKMKTHKSAAKRFRVTKNGKIIRRKAYAWHKTGKKRRSTLRRLKVETEVKACDKDRVLRMLGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHT6</accession>
    <name>B7IHT6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translation initiation factor IF-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">infC</name>
        <name evidence="7" type="ordered locus">THA_1200</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the IF-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1200"/>
    <dbReference type="EnsemblBacteria" id="ACJ75650">
        <property type="protein sequence ID" value="ACJ75650"/>
        <property type="gene ID" value="THA_1200"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1200"/>
    <dbReference type="eggNOG" id="COG0290">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054919_3_2_0"/>
    <dbReference type="OMA" id="RNMIMFL"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036788">
        <property type="entry name" value="T_IF-3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036787">
        <property type="entry name" value="T_IF-3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019813">
        <property type="entry name" value="Translation_initiation_fac3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001288">
        <property type="entry name" value="Translation_initiation_fac_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019815">
        <property type="entry name" value="Translation_initiation_fac_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019814">
        <property type="entry name" value="Translation_initiation_fac_3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10938">
        <property type="entry name" value="PTHR10938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00707">
        <property type="entry name" value="IF3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05198">
        <property type="entry name" value="IF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54364">
        <property type="entry name" value="SSF54364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55200">
        <property type="entry name" value="SSF55200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00168">
        <property type="entry name" value="infC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00938">
        <property type="entry name" value="IF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF3_N" evidence="6">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="IF3_C" evidence="5">
        <location>
            <begin position="55"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="141" mass="16533" checksum="DA11C1BEC28CDC9B" modified="2009-02-10" version="1">MPTQKALELAYSQKLDLILVAPNAKPPVAKIMDYGKYKYELAKREKKAKKNQKIIEVKQMKFRIKIDEHDYQTKLKHIKRFLEAGNKVRVVIMFRGREMAFADKGKEILDRIIKDLQDVASVEKAPKLEGRDMWMMLKPKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHU6</accession>
    <name>RL3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplC</name>
        <name type="ordered locus">THA_1215</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L19.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101438.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHU6"/>
    <dbReference type="SMR" id="B7IHU6"/>
    <dbReference type="STRING" id="484019.THA_1215"/>
    <dbReference type="EnsemblBacteria" id="ACJ75660">
        <property type="protein sequence ID" value="ACJ75660"/>
        <property type="gene ID" value="THA_1215"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1215"/>
    <dbReference type="eggNOG" id="COG0087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044142_4_1_0"/>
    <dbReference type="OMA" id="KRMAGRY"/>
    <dbReference type="OrthoDB" id="1270636at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_B">
        <property type="entry name" value="Ribosomal_L3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_L3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019927">
        <property type="entry name" value="Ribosomal_L3_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_L3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229">
        <property type="entry name" value="PTHR11229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03625">
        <property type="entry name" value="L3_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000141933" description="50S ribosomal protein L3">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <sequence length="205" mass="22592" checksum="3AA4EA15FC95CF21" modified="2009-02-10" version="1">MKMIIGRKVGMTRIFKDDKVIPVTVIKAGPCYVVQKKTIDTDGYNAIQIGFEEAKKVNKPMEGVFKKAGVKPLKILKEFRVENPEEFELGQEIKVDIFAEGDKIDITGWSKGRGFAGAMKRWGFRGGPKSHGAKFHRELGSVGQHSEPARIFKGKKMPGQYGNERVTILNSEIVKIDVENNLIAVKGGVPGARGGLVLIRTAKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMS8</accession>
    <name>C0QMS8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase repeat family ISCc3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_2002</name>
        <name evidence="2" type="ordered locus">THA_2003</name>
        <name evidence="3" type="ordered locus">THA_2008</name>
        <name evidence="4" type="ordered locus">THA_2009</name>
        <name evidence="5" type="ordered locus">THA_2010</name>
        <name evidence="6" type="ordered locus">THA_2013</name>
    </gene>
    <organism evidence="1 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2002"/>
    <dbReference type="EnsemblBacteria" id="ACN29495">
        <property type="protein sequence ID" value="ACN29495"/>
        <property type="gene ID" value="THA_2002"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29496">
        <property type="protein sequence ID" value="ACN29496"/>
        <property type="gene ID" value="THA_2003"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29501">
        <property type="protein sequence ID" value="ACN29501"/>
        <property type="gene ID" value="THA_2008"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29502">
        <property type="protein sequence ID" value="ACN29502"/>
        <property type="gene ID" value="THA_2009"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29503">
        <property type="protein sequence ID" value="ACN29503"/>
        <property type="gene ID" value="THA_2010"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29506">
        <property type="protein sequence ID" value="ACN29506"/>
        <property type="gene ID" value="THA_2013"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2002"/>
    <dbReference type="KEGG" id="taf:THA_2003"/>
    <dbReference type="KEGG" id="taf:THA_2008"/>
    <dbReference type="KEGG" id="taf:THA_2009"/>
    <dbReference type="KEGG" id="taf:THA_2010"/>
    <dbReference type="KEGG" id="taf:THA_2013"/>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_36_0_0"/>
    <dbReference type="OMA" id="ADICMEH"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACN29495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACN29496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACN29501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACN29502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACN29503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACN29506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="96" mass="10892" checksum="45A38CB7D7842ED5" modified="2009-05-05" version="1">MRKKFSAEEKMAIVLEGLRGKKTIAEICRKHSISQGQYYKWRDKFLEGAKTALETSGNSSQAAQYQRKIQELEQIIGKQAIVIETLKKTIIHPGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMT1</accession>
    <name>C0QMT1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase repeat family IS200</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_2005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100532.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2005"/>
    <dbReference type="EnsemblBacteria" id="ACN29498">
        <property type="protein sequence ID" value="ACN29498"/>
        <property type="gene ID" value="THA_2005"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2005"/>
    <dbReference type="eggNOG" id="COG1943">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101320_2_2_0"/>
    <dbReference type="OMA" id="WNCKYHV"/>
    <dbReference type="OrthoDB" id="1547701at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002686">
        <property type="entry name" value="Transposase_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036515">
        <property type="entry name" value="Transposase_17_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01797">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01321">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143422">
        <property type="entry name" value="SSF143422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Y1_Tnp" evidence="1">
        <location>
            <begin position="10"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACN29498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="151" mass="17916" checksum="C1DC0E6124314E7B" modified="2009-05-05" version="1">MFLESNNHSVFKLNYHLVLVTKYRRSVIDDNISDFLKKIFEYIGEKYGIKLQEWNHDKDHIHVLFSAQPNSQLSKFINAYKSTSSRLVKKKFPQIKSKLWKSAFWSKSYCLLTVGEAPLEVLKEYIKSQGEDKRASNLQISYLSYERTRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMU0</accession>
    <name>C0QMU0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase repeat family IS200</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_2014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580544.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2014"/>
    <dbReference type="EnsemblBacteria" id="ACN29507">
        <property type="protein sequence ID" value="ACN29507"/>
        <property type="gene ID" value="THA_2014"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2014"/>
    <dbReference type="eggNOG" id="COG1943">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101320_2_2_0"/>
    <dbReference type="OMA" id="DKAFWQP"/>
    <dbReference type="OrthoDB" id="1547701at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002686">
        <property type="entry name" value="Transposase_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036515">
        <property type="entry name" value="Transposase_17_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01797">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01321">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143422">
        <property type="entry name" value="SSF143422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Y1_Tnp" evidence="1">
        <location>
            <begin position="10"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACN29507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="153" mass="18035" checksum="1A5D3DB1D019917A" modified="2009-05-05" version="1">MLLESNNHSVFKLNYHLLLVTKYRRSVIDDKISDFLKKIFQYIGEKYGIKLQEWNHDKDHIHVLFSAQPNSQLSKFINAYKSASSRLVKKKFPQIKSKLWKSAFWSKSYCLLTVGGAPLEVLKEYIKSQGEDKRASNLQISYLSYERTRRKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMU5</accession>
    <name>C0QMU5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_2019</name>
        <name evidence="4" type="ordered locus">THA_2024</name>
        <name evidence="5" type="ordered locus">THA_2042</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580547.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2019"/>
    <dbReference type="EnsemblBacteria" id="ACN29512">
        <property type="protein sequence ID" value="ACN29512"/>
        <property type="gene ID" value="THA_2019"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29517">
        <property type="protein sequence ID" value="ACN29517"/>
        <property type="gene ID" value="THA_2024"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29535">
        <property type="protein sequence ID" value="ACN29535"/>
        <property type="gene ID" value="THA_2042"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2019"/>
    <dbReference type="KEGG" id="taf:THA_2024"/>
    <dbReference type="KEGG" id="taf:THA_2042"/>
    <dbReference type="eggNOG" id="COG3547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036902_4_1_0"/>
    <dbReference type="OMA" id="IGDTDAM"/>
    <dbReference type="OrthoDB" id="655500at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transposase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEDD_Tnp_IS110" evidence="2">
        <location>
            <begin position="20"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="258"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACN29512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACN29517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACN29535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="419" mass="48914" checksum="FC97BB320F60A964" modified="2009-05-05" version="1">MKQKSINKKISRITQDTLIVGIDVAKRTHWIRMMDFRGIDLIKPFKINNTMDGIKMLEEKIKSVKEENKLNKVVLGMEPSGHYWKVLAWQMKISKEIDYLVGVNPYHVKKSKEFDDNSPGKSDRKDAGVIARLIRDGRYFDIYLPEDIYGELRVITTTREQLVNKRKNAKNMVISVMDEYFPEYEKIYKNIFSEGSIKLLKTHPFPKEIIEVGIEEIEKVLKKSTNGKDWKSRAQKIYESAKKSIGVREGQKSAKIKLRMLLEEIEFLTSQIEELEKEMEELVEETEEGEYIKSVPGIGPIMTAVILGEIGDINRFKSWKEIRKLAGLNLYEISSGEHKGKTRITKRGRPLLRKIIYLMAQTTLKHNHEIRKKYEQLRRREKNPLKVVQALIAIGLKMIRIVFKLAKSKIKYEPEKVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMW3</accession>
    <name>C0QMW3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase repeat family ISChy1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_2037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580552.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29530">
        <property type="protein sequence ID" value="ACN29530"/>
        <property type="gene ID" value="THA_2037"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2037"/>
    <dbReference type="eggNOG" id="COG3547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036902_4_4_0"/>
    <dbReference type="OMA" id="MEATGCY"/>
    <dbReference type="OrthoDB" id="655500at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transposase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEDD_Tnp_IS110" evidence="1">
        <location>
            <begin position="20"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACN29530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="382" mass="43893" checksum="30EB782FDD52F273" modified="2009-05-05" version="1">MAILAIDVSKSSLSFYSDFIGKGFVDNSPQGIFDLFNKASSSSSDFILVLESTGVYSFDVANYFFQNHIPVFWVKTDNMKLYRKILNRPKTDKIDAKLIFDIASKFPDSLVPFVNNSYIISELRNLTRLYLKLNEDIARLKLRLYSYVSLYFPKLDFKLNKTYESLLKDFTVEEIATMPLEELFEYIAKVSRHIVSSKDLAEKIQSLAKNALRLSPNPSNTLRLSIVSTIELIQHYEKQINLVKKEISKLIKRIPNTLTTVKGIGDITAAGIIAEIGDINRFRNASALASYAGLVWNIKQSGNYKSENTQLTKKGNKYLRTYLVMAANSLRIYDPIFKDYYQRKYTQTTTHKHMRALILSARKLVNLVYYLLKNNVPYVPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICL2</accession>
    <name>B7ICL2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1294</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101579.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1294"/>
    <dbReference type="EnsemblBacteria" id="ACJ75739">
        <property type="protein sequence ID" value="ACJ75739"/>
        <property type="gene ID" value="THA_1294"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1294"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_0"/>
    <dbReference type="OMA" id="IVTVWQY"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="69"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="293" mass="33228" checksum="506741377A26DE6E" modified="2009-02-10" version="1">MKKKELREIGKGLIFISPWLIGFIVFTLIPFIASFYYSFTEFSITTDVKWVGTENYKRIFNDPLFWKSLKNTLVFAIGLVPLGLVVALSTAFLMNKPLKEVSFYRAAIYMPSIVPAFAFSAVVTWFFHPYLGLVNSILELFGINGPLWLSSEKWVKPTIIIAAQWGVGSAMLIFSAALKDIPKELYESAKIDGAKSFTMFRKITLPLITPTILYYLITSTIGALQIFDLPMLLTNGGPANASMSLSMYIYKHAFTYTNMGYASALSWILFFLTFVLTIIYFITSKKWVYYFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICN3</accession>
    <name>B7ICN3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ribosomal protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101637.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1315"/>
    <dbReference type="EnsemblBacteria" id="ACJ75760">
        <property type="protein sequence ID" value="ACJ75760"/>
        <property type="gene ID" value="THA_1315"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1315"/>
    <dbReference type="eggNOG" id="COG2868">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140910_1_0_0"/>
    <dbReference type="OMA" id="HSHFAPK"/>
    <dbReference type="OrthoDB" id="2065603at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16332">
        <property type="entry name" value="Prp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007422">
        <property type="entry name" value="Peptidase_Prp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036764">
        <property type="entry name" value="Peptidase_Prp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39178">
        <property type="entry name" value="PTHR39178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04327">
        <property type="entry name" value="Peptidase_Prp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118010">
        <property type="entry name" value="SSF118010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="103" mass="11765" checksum="F926C8F3D6F9042B" modified="2009-02-10" version="1">MITCKFFKENGFFEKFLITGHSMYAQKGKDIVCAAVSVVAQHTARYLLKNGASVKIKDGYLDVKNISHDNISQIFVNELIDTLKDLEEQYPKYLKLEVSNDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICT1</accession>
    <name>B7ICT1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metal-dependent carboxypeptidase</fullName>
            <ecNumber evidence="1">3.4.17.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1363</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of a C-terminal amino acid with broad specificity, except for -Pro.</text>
            <dbReference type="EC" id="3.4.17.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M32 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.19" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580179.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1363"/>
    <dbReference type="EnsemblBacteria" id="ACJ75808">
        <property type="protein sequence ID" value="ACJ75808"/>
        <property type="gene ID" value="THA_1363"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1363"/>
    <dbReference type="eggNOG" id="COG2317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032916_1_1_0"/>
    <dbReference type="OMA" id="EFGHALY"/>
    <dbReference type="OrthoDB" id="239313at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001333">
        <property type="entry name" value="Peptidase_M32_Taq"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34217">
        <property type="entry name" value="PTHR34217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02074">
        <property type="entry name" value="Peptidase_M32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006615">
        <property type="entry name" value="Zn_crbxpep_Taq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00998">
        <property type="entry name" value="CRBOXYPTASET"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR006615-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="489" mass="57911" checksum="8733699E7E1BF88B" modified="2009-02-10" version="1">MQELKNHLKKLSKFESALSLLHWDMETYMPPNAVEKRAQIIGEISGYVFEQFISEKTQELIEKAQPNDVESQAILRLVKKEYERMKKIPKELFVELQIETSKAQQAWQNAKKEKDFNLFKPNLKKVVELEKKVIEVLGYEENKYDTLLDQYEPGLKTKQLNEIIPPLRSFLVEFLENLSQGKQPNTEILEGEYDETLQKRFSEELLKALNYDFNSGRLDVSAHPFTISISHNDVRITTRFDRFDIKNSIFSTIHECGHALYELGIPEEYRELPIGDGASMGIHESQSRFWENIVGRSFEFWKFMYPKFVKYFPRFEKIDIKDFWRAINKVERSLIRTEADEVTYNLHIMIRFEIEEALINDRINVDELPLIWNEKMKEYLGISPKNDSEGVLQDVHWAHGSFGYFPSYMLGNLYAAQIFKQMKKDIPDFYQKVENGEFKPILNWLREKIHSKGKVYQPMELIKMVTGETLNPQYFIDYVSEKYKEVYML</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICT2</accession>
    <name>B7ICT2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580180.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1364"/>
    <dbReference type="EnsemblBacteria" id="ACJ75809">
        <property type="protein sequence ID" value="ACJ75809"/>
        <property type="gene ID" value="THA_1364"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1364"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_997250_0_0_0"/>
    <dbReference type="OMA" id="NWQREPI"/>
    <dbReference type="OrthoDB" id="1526822at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="282" mass="32845" checksum="F4107012DBE67822" modified="2009-02-10" version="1">MNLKILTLNEYSLIDFVNLVNKVFEDYTVPVNWNVINFQLDARENSISLEDSFIFFKGEKPVGFIVNSIRRDRARIDAMGVVKEERGTGTASFILEHATNYLKWKGIKKILLEVITKDKRAYRFYEKHGFREKRKLHLLVNKDLKTESIEYRAIKVEPRYVYTLSLNLEISGRTTNWQREPITLLLADGRYNYVKLVHNGKEGYLVWGKNEDKSTYIVDLGTKNNDEWEIFAKIAVDNLVRETNCKIISATSVPENDPLYSALKNTGFTSLIEQSEMYKNIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICT3</accession>
    <name>B7ICT3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribosomal protein L7A family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580181.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1365"/>
    <dbReference type="EnsemblBacteria" id="ACJ75810">
        <property type="protein sequence ID" value="ACJ75810"/>
        <property type="gene ID" value="THA_1365"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1365"/>
    <dbReference type="eggNOG" id="COG1358">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157804_2_0_0"/>
    <dbReference type="OMA" id="IRCEINN"/>
    <dbReference type="OrthoDB" id="2066583at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_L7Ae/L30e/S12e/Gad45"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L7Ae" evidence="1">
        <location>
            <begin position="5"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="105" mass="11820" checksum="7345003477F3332D" modified="2009-02-10" version="1">MNEQKILTLLGFASKAKKLVYGKDNIRDYIKNPKLKNKLIIMAKDTGERVKKDIKIRCDISNVPLIEFSDKKTISKATGMENVAVIGIIDENMVKSILEFYSKGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICT4</accession>
    <name>GCH4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP cyclohydrolase FolE2</fullName>
            <ecNumber evidence="1">3.5.4.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">folE2</name>
        <name type="ordered locus">THA_1366</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts GTP to 7,8-dihydroneopterin triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase IV family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580182.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ICT4"/>
    <dbReference type="SMR" id="B7ICT4"/>
    <dbReference type="STRING" id="484019.THA_1366"/>
    <dbReference type="EnsemblBacteria" id="ACJ75811">
        <property type="protein sequence ID" value="ACJ75811"/>
        <property type="gene ID" value="THA_1366"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1366"/>
    <dbReference type="eggNOG" id="COG1469">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062816_1_1_0"/>
    <dbReference type="OMA" id="PCSQGMS"/>
    <dbReference type="OrthoDB" id="757842at2"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035998">
        <property type="term" value="P:7,8-dihydroneopterin 3'-triphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01527_B">
        <property type="entry name" value="GTP_cyclohydrol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022838">
        <property type="entry name" value="GTP_cyclohydrolase_FolE2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003801">
        <property type="entry name" value="GTP_cyclohydrolase_FolE2/MptA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36445">
        <property type="entry name" value="PTHR36445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02649">
        <property type="entry name" value="GCHY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00294">
        <property type="entry name" value="TIGR00294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000185158" description="GTP cyclohydrolase FolE2">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="site" description="May be catalytically important" evidence="1">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01527"/>
        </source>
    </evidence>
    <sequence length="259" mass="30260" checksum="91B935EF72AAC080" modified="2009-02-10" version="1">MRDVQSERDERNVPLKHVGIKNLKYPVVVLDKKNKNQHTIAEINMYVDLPKDFRGTHMSRFLEVLNKFHLKIDPKNIKAILDDLKKTLKAQSASIEIMFPYFLQKKAPVTKIESYMEYKCGFKAYDTNEECEFYIVVEVPIQTLCPCSKEISKYNAHNQRALARIEVETSELIWFEDLIELAESSASVPLFTLLKRPDEKYVTEKAYENPKFVEDVARDIALSLKENKKIRWFKVEVESFESIHNHNAYACVNSDTMEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICU5</accession>
    <name>B7ICU5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580191.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1377"/>
    <dbReference type="EnsemblBacteria" id="ACJ75822">
        <property type="protein sequence ID" value="ACJ75822"/>
        <property type="gene ID" value="THA_1377"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1377"/>
    <dbReference type="eggNOG" id="ENOG5034BNM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1452425_0_0_0"/>
    <dbReference type="OMA" id="IAYRYLY"/>
    <dbReference type="OrthoDB" id="1466922at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="177" mass="20577" checksum="DB114B9C7FEF7910" modified="2009-02-10" version="1">MKNRLLFTLLLILSAGILFGNLYTDAYNYTYKVAKYYGVNDERAKVIAQTVAEEYEKFPQVPYQIFVAVIVSESGFKNLYGDSGHAVGYCQLHESATDYVALFFPEIKHILSKISHDDLIKFPALQIRIAYRYLYLIMKNITNYNIIKALNFWNNSDKYYLRVFDTLVFIDSIAMKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICU7</accession>
    <name>B7ICU7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1379</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101762.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1379"/>
    <dbReference type="EnsemblBacteria" id="ACJ75824">
        <property type="protein sequence ID" value="ACJ75824"/>
        <property type="gene ID" value="THA_1379"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1379"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_9_1_0"/>
    <dbReference type="OMA" id="GIGLEYC"/>
    <dbReference type="OrthoDB" id="968232at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="216" mass="23759" checksum="A988DD8733839CB9" modified="2009-02-10" version="1">MNILITGANRGIGYALLQEALKRGHLVIAATRKPEMIKIDNNRVSVYFLDLLDKDSIEKFVETLSVEVDALINNAGVLYKDSFENLEYDYFLNTFKVNTLGPLFLSQRLYKSGKLKSGGKIINISSILGSIALLGGTTSYSYSVSKAALNMVTKLLSSKLKDIKVISVHPGWVKTDMGGKEAPVMPEESAKGIMDIIENVEESGVFLDYTGKSLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICV0</accession>
    <name>B7ICV0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome c-type biogenesis protein CcdA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1382</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580194.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1382"/>
    <dbReference type="EnsemblBacteria" id="ACJ75827">
        <property type="protein sequence ID" value="ACJ75827"/>
        <property type="gene ID" value="THA_1382"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1382"/>
    <dbReference type="eggNOG" id="COG0785">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053225_2_0_0"/>
    <dbReference type="OMA" id="TSLTPCM"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003834">
        <property type="entry name" value="Cyt_c_assmbl_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02683">
        <property type="entry name" value="DsbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="DsbD" evidence="2">
        <location>
            <begin position="11"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="212" mass="23082" checksum="84B266B90D8594F3" modified="2009-02-10" version="1">MFYKDVSIWIAFLHGILSFFSPCVLPLIPAFLGVLFTAKNKFLKLFGFFIGFSVFFSIIGIFSSVFGIFFSKYGTVINYILGTIIIVMGILYMSNKEIVKPKKINVWNFKGGSLLTGILMGAAVGLIWIPCSSPILGSILTIATTVNPYKGGLLLFIYSLGISLPFLTIGAPISKLLTGGFGTPKWEKILKIFGGVFLIILGILIISGKMVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICV2</accession>
    <name>B7ICV2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1384</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101767.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1384"/>
    <dbReference type="EnsemblBacteria" id="ACJ75829">
        <property type="protein sequence ID" value="ACJ75829"/>
        <property type="gene ID" value="THA_1384"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1384"/>
    <dbReference type="eggNOG" id="COG0695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_9_3_0"/>
    <dbReference type="OMA" id="IFTTPTC"/>
    <dbReference type="OrthoDB" id="2045232at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097573">
        <property type="term" value="F:glutathione oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011911">
        <property type="entry name" value="GlrX_YruB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02196">
        <property type="entry name" value="GlrX_YruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="1">
        <location>
            <begin position="6"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="80" mass="9124" checksum="EF206FDC2EFEB2E1" modified="2009-02-10" version="1">MQHVKIVVYTTPTCPYCKKAKNYFKQLGLKFKEYDVSKDQKAAERMYKKSGQLGVPVIEIGNQIVVGFDKAKIDRLLGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICW0</accession>
    <name>B7ICW0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Dipeptide ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1393</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101778.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1393"/>
    <dbReference type="EnsemblBacteria" id="ACJ75837">
        <property type="protein sequence ID" value="ACJ75837"/>
        <property type="gene ID" value="THA_1393"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1393"/>
    <dbReference type="eggNOG" id="COG4608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_0"/>
    <dbReference type="OMA" id="CPIAMDV"/>
    <dbReference type="OrthoDB" id="1303270at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="12"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="55"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="333" mass="38114" checksum="F2A8F0E7B6BAC72C" modified="2009-02-10" version="1">MQKTIYKTDTILKIENLKKYFAADRGLFSRVKHFVHAVDDVSFEIKRGKSLGLVGESGCGKTTTGKVIVGLEKPTDGKVYIDGKEISEYESKLEYHKKVQMIFQDPYESLNPRMTIFDIIAEPLNIHNIGNLKEREEKVLKLLQDVGLTPPSSFLWRYPHELSGGQRQRVAIARALVLNPTFIVADEPTSMLDVSVRTGVMNLLMELQEKYNMSYLYITHDFAVARYMCDKIAVMYLGKIVEYAETEELLFNPMHPYTRALLTAVPVPDPEHKKDEPNIIGSVSKPINPPPRCRFYDRCPFRTEKCKNEPHPQLKDIGNNHYVACYLVQEGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICY7</accession>
    <name>B7ICY7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580216.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1420"/>
    <dbReference type="EnsemblBacteria" id="ACJ75864">
        <property type="protein sequence ID" value="ACJ75864"/>
        <property type="gene ID" value="THA_1420"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1420"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2206133_0_0_0"/>
    <dbReference type="OMA" id="IDEPYTE"/>
    <dbReference type="OrthoDB" id="1995303at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="105" mass="11971" checksum="E8A150062F24E9F3" modified="2009-02-10" version="1">MKTNEIKKGEKIFEEGQEANSVYLIKSGEVVVKFGQLEEKLEKNDVIGIESLINENYTESAIAHTDVKLTELTPEEFKELYSGTDVEKKAIKSFTKRTMKLLGWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICZ6</accession>
    <name>B7ICZ6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Flagellar basal body rod protein FlgB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">flgB</name>
        <name evidence="7" type="ordered locus">THA_1429</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Structural component of flagellum, the bacterial motility apparatus. Part of the rod structure of flagellar basal body.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The basal body constitutes a major portion of the flagellar organelle and consists of a number of rings mounted on a central rod.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101850.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1429"/>
    <dbReference type="EnsemblBacteria" id="ACJ75873">
        <property type="protein sequence ID" value="ACJ75873"/>
        <property type="gene ID" value="THA_1429"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1429"/>
    <dbReference type="eggNOG" id="COG1815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125463_3_1_0"/>
    <dbReference type="OMA" id="GHMARND"/>
    <dbReference type="OrthoDB" id="1914857at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030694">
        <property type="term" value="C:bacterial-type flagellum basal body, rod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006300">
        <property type="entry name" value="FlgB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30435:SF12">
        <property type="entry name" value="PTHR30435:SF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002889">
        <property type="entry name" value="Rod_FlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01396">
        <property type="entry name" value="FlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="127" mass="14631" checksum="42FC3E8CABC3C45F" modified="2009-02-10" version="1">MFNSNFSTLKAAMDVALKRQEIHSQNIANAETPNYKRKYIVFEELLNESKMKLSLKTTSGKHIERTPKVVNPIIKAQKNTSLTNDGNNVDIDIEMVEMVKNALRYQTLSRLMSANIDRYNTVIRNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICZ8</accession>
    <name>B7ICZ8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellar hook-basal body complex protein FliE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">fliE</name>
        <name evidence="4" type="ordered locus">THA_1431</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the FliE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101854.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1431"/>
    <dbReference type="EnsemblBacteria" id="ACJ75875">
        <property type="protein sequence ID" value="ACJ75875"/>
        <property type="gene ID" value="THA_1431"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1431"/>
    <dbReference type="eggNOG" id="COG1677">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147249_3_4_0"/>
    <dbReference type="OMA" id="LHDVMIT"/>
    <dbReference type="OrthoDB" id="1581972at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00724">
        <property type="entry name" value="FliE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001624">
        <property type="entry name" value="FliE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34653">
        <property type="entry name" value="PTHR34653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02049">
        <property type="entry name" value="FliE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01006">
        <property type="entry name" value="FLGHOOKFLIE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00205">
        <property type="entry name" value="fliE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="95" mass="10701" checksum="12F4272DACA0BC5E" modified="2009-02-10" version="1">MIDKINGVGNYGNLKPSSVKKQNTDFTKILNDALKSVNDQQKKAEQMADDFATGKISNIHEVIVEAEKASVSLRLTVEVRNKIVDAYKEIMRMQF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID00</accession>
    <name>DNAK_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperone protein DnaK</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">HSP70</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heat shock 70 kDa protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heat shock protein 70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaK</name>
        <name type="ordered locus">THA_1433</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a chaperone.</text>
    </comment>
    <comment type="induction">
        <text evidence="1">By stress conditions e.g. heat shock.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101858.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ID00"/>
    <dbReference type="SMR" id="B7ID00"/>
    <dbReference type="STRING" id="484019.THA_1433"/>
    <dbReference type="PRIDE" id="B7ID00"/>
    <dbReference type="EnsemblBacteria" id="ACJ75877">
        <property type="protein sequence ID" value="ACJ75877"/>
        <property type="gene ID" value="THA_1433"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1433"/>
    <dbReference type="eggNOG" id="COG0443">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_2_4_0"/>
    <dbReference type="OMA" id="ISIKRHM"/>
    <dbReference type="OrthoDB" id="161217at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="PTHR19375"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="SSF100920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="SSF100934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_1000119766" description="Chaperone protein DnaK">
        <location>
            <begin position="1"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine; by autocatalysis" evidence="1">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00332"/>
        </source>
    </evidence>
    <sequence length="598" mass="66259" checksum="2AC380DCC81A3ED3" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID40</accession>
    <name>B7ID40_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="7">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">purC</name>
        <name evidence="9" type="ordered locus">THA_1474</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580250.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1474"/>
    <dbReference type="EnsemblBacteria" id="ACJ75917">
        <property type="protein sequence ID" value="ACJ75917"/>
        <property type="gene ID" value="THA_1474"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1474"/>
    <dbReference type="eggNOG" id="COG0152">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061495_0_0_0"/>
    <dbReference type="OMA" id="EFCYKND"/>
    <dbReference type="OrthoDB" id="1345271at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR_synt" evidence="8">
        <location>
            <begin position="2"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="228" mass="26226" checksum="D82D2AE540B50D29" modified="2009-02-10" version="1">MEGKTKIVEDFGDYVILNFKDDITAGDGEKHNVLEGKGAICAEITAISMKYLNSRGIYTQFLDFITPNKIKAVSLKMLPLEVVVRLKKAGSFIRRYGGNEGEEFKEPLVEFFIKDDERHDPMVCKNHLVLFEICNEKQAEEIEKSAKKTAIELKNFFEKLGFDLWDMKFEYGIDNEGKVCLGDEISPDTLRLRKKDGIFDKDVYRKYLGDPLEKYREVLSACRSLNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID52</accession>
    <name>B7ID52_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NTP_transf_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1488</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101971.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1488"/>
    <dbReference type="EnsemblBacteria" id="ACJ75929">
        <property type="protein sequence ID" value="ACJ75929"/>
        <property type="gene ID" value="THA_1488"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1488"/>
    <dbReference type="eggNOG" id="COG2068">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061980_1_1_0"/>
    <dbReference type="OMA" id="DKAVWKL"/>
    <dbReference type="OrthoDB" id="1432587at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="1">
        <location>
            <begin position="13"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="194" mass="22476" checksum="97ABA75B0BA42FBD" modified="2009-02-10" version="1">MERISFEFRIFSAILAAGQGKRFQNNVKILHNINGKPMLQHVIDVVKSINFEKNFLVVNPLWNKINPHFIIPKSFTILINKEYEKGISTSLKLLIKNIIPFEPDYIAIFLADMPYISTDIVYNILSKIEKEDKIIAPYYKDIKGFPTIVHKSLFQNILNLQGDSGIKQIIYKNPNLVKKIEFKTDKVIKDIDQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID54</accession>
    <name>B7ID54_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580256.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="SMR" id="B7ID54"/>
    <dbReference type="STRING" id="484019.THA_1490"/>
    <dbReference type="EnsemblBacteria" id="ACJ75931">
        <property type="protein sequence ID" value="ACJ75931"/>
        <property type="gene ID" value="THA_1490"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1490"/>
    <dbReference type="eggNOG" id="COG0657">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_4_0_0"/>
    <dbReference type="OMA" id="QGSAWTT"/>
    <dbReference type="OrthoDB" id="1470522at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S9" evidence="2">
        <location>
            <begin position="128"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="299" mass="34851" checksum="2495E8E51673287D" modified="2009-02-10" version="1">MNLKIFFKYIVYIFLIILSFINFGLTLLIIFILQIPVIRKSILGRLPFKLEKEPWSKKETFEYKKNLWLDVYFPKEKSEHLILFAHGGGWISGYRRQPNNVSWYRFLVSKGFIVATIDYRYGFLNEIEQLVEDLSDAYNFVKNNINVKKISLMGLSAGGHLALYFGLKHKVKLENIVSYYSPCDLLDIWKSESLFARFAVSTTLKRLPSKSKDVYKYYSPISYVEKGVVPILLVHGMKDKVVPYISSVKMYKKLRSFSNKAKLLLHPFGDHGFEFILKDEKTKSILLKTVDFLKGDGYD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID85</accession>
    <name>NRDR_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcriptional repressor NrdR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nrdR</name>
        <name type="ordered locus">THA_1521</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR-boxes.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NrdR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102050.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ID85"/>
    <dbReference type="SMR" id="B7ID85"/>
    <dbReference type="STRING" id="484019.THA_1521"/>
    <dbReference type="EnsemblBacteria" id="ACJ75962">
        <property type="protein sequence ID" value="ACJ75962"/>
        <property type="gene ID" value="THA_1521"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1521"/>
    <dbReference type="eggNOG" id="COG1327">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108412_0_0_0"/>
    <dbReference type="OMA" id="YRFTTYE"/>
    <dbReference type="OrthoDB" id="1551627at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00440">
        <property type="entry name" value="NrdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003796">
        <property type="entry name" value="RNR_NrdR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30455">
        <property type="entry name" value="PTHR30455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00244">
        <property type="entry name" value="TIGR00244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_1000124560" description="Transcriptional repressor NrdR">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-cone" evidence="1">
        <location>
            <begin position="49"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="zinc finger region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00440"/>
        </source>
    </evidence>
    <sequence length="151" mass="17649" checksum="89FF03EB178D6D51" modified="2009-02-10" version="1">MKCPYCGNDETRVLDSRVDSSGMTIRRRRECLKCKGRFTTYERYEFGPVFVIKKDGRREKFDRSKILSGIMKACEKTNVTLEEMEKLVDDVVNQIQKTGNLEISSLEIGKLVMEGLKKINEVAYVRFASVYKDFREIDQFLEVVKELKNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID97</accession>
    <name>B7ID97_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1533</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580265.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1533"/>
    <dbReference type="EnsemblBacteria" id="ACJ75974">
        <property type="protein sequence ID" value="ACJ75974"/>
        <property type="gene ID" value="THA_1533"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1533"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_0_0"/>
    <dbReference type="OMA" id="MWRAFKK"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="459"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="510"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="333"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="542" mass="61997" checksum="A6180BC3BE33B38C" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDI0</accession>
    <name>RRF_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome-recycling factor</fullName>
            <shortName evidence="1">RRF</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosome-releasing factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">frr</name>
        <name type="ordered locus">THA_1619</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580300.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDI0"/>
    <dbReference type="SMR" id="B7IDI0"/>
    <dbReference type="STRING" id="484019.THA_1619"/>
    <dbReference type="EnsemblBacteria" id="ACJ76057">
        <property type="protein sequence ID" value="ACJ76057"/>
        <property type="gene ID" value="THA_1619"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1619"/>
    <dbReference type="eggNOG" id="COG0233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073981_2_0_0"/>
    <dbReference type="OMA" id="FNPMNNG"/>
    <dbReference type="OrthoDB" id="1674344at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00520">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00040">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="PTHR20982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="SSF55194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00496">
        <property type="entry name" value="frr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000194962" description="Ribosome-recycling factor">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00040"/>
        </source>
    </evidence>
    <sequence length="185" mass="21525" checksum="922B1F1D02B49A4D" modified="2009-02-10" version="1">MKDPILKEAELRMKKSVEAIDEELKKLRTGRPSPALLEEIKIDYYGVPTPINQVATINVTEERSLIIKPWEKNLLSAIEKAIQASDLGLNPTNDGNVVRLVFPSPTTEQRQKWVKKTKEIVEHGKIAVRNIRRDVIKELKEMTKNGEISEDDEKRLEKEVQNLTDKYIEELDKLFERKEKEIMEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDL0</accession>
    <name>B7IDL0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polysaccharide pyruvyl transferase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580326.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1649"/>
    <dbReference type="EnsemblBacteria" id="ACJ76087">
        <property type="protein sequence ID" value="ACJ76087"/>
        <property type="gene ID" value="THA_1649"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1649"/>
    <dbReference type="eggNOG" id="COG2327">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039510_0_1_0"/>
    <dbReference type="OMA" id="VISERFH"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007345">
        <property type="entry name" value="Polysacch_pyruvyl_Trfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04230">
        <property type="entry name" value="PS_pyruv_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PS_pyruv_trans" evidence="1">
        <location>
            <begin position="14"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="322" mass="37230" checksum="BB38D78AB757FAF7" modified="2009-02-10" version="1">MKNVLLIGYYGYGNYGDELMRQSIKEFLIEKNYRVMELLPKEKKKLNEYNRFNPLSIIRAIIKSDVVICGGGGVLQDKTSFKSFMYYYLLFKTALLFGKPLIFFGNSFGPFYNYASRILMKNLLESKKLYIFARDPVSYRYAKNFNKNSFLCTDPGIRKLGSIKVESSKESNKAVIIPRRVKNYVPLLLTLKSQGINEVVYVPFSPQDEVLAKRLSNISIKGLNSRFSNLIIEEISSSKIVVSERFHGALVSAFLGKAFISVNDEKFRRFFNRYSSNKSHFAHDLFDASLKVKELVNPNIYKKMKEDAEEMYEKLEKLLVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDL1</accession>
    <name>B7IDL1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hemin transport system ATP-binding protein HmuV</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1650</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580327.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1650"/>
    <dbReference type="EnsemblBacteria" id="ACJ76088">
        <property type="protein sequence ID" value="ACJ76088"/>
        <property type="gene ID" value="THA_1650"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1650"/>
    <dbReference type="eggNOG" id="COG1120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_0"/>
    <dbReference type="OMA" id="FHIDAEI"/>
    <dbReference type="OrthoDB" id="1752365at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018078">
        <property type="entry name" value="DNA-binding_RecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00618">
        <property type="entry name" value="RECF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="254" mass="28966" checksum="AFE806D4EF264CEE" modified="2009-02-10" version="1">MNEILKVINLNFSYGNFGIKNLNLNIKEGQFVGIIGPNGAGKSTTLNLIAGLLKPYSGKILLYDKDIRKLSRKYIASKISFVRQEFNPTFEFKVKDIIEMGALHKKTSFFNTFEDSTKIKECLSMVELLGYEDRYFSTLSGGEQRRVLIARSIYQETPVIIVDELTAHLDISHAIQIVEILKKLTLKGKTVLSAFHNINVASKYCNYIYALKDGKVIFEGAPIKVINKENIKKLYEKEFYIINHPVHNYPVVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDM1</accession>
    <name>B7IDM1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable hypoxanthine oxidase XdhD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580333.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1660"/>
    <dbReference type="EnsemblBacteria" id="ACJ76098">
        <property type="protein sequence ID" value="ACJ76098"/>
        <property type="gene ID" value="THA_1660"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1660"/>
    <dbReference type="eggNOG" id="COG1529">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_1_0"/>
    <dbReference type="OMA" id="SWMRAPG"/>
    <dbReference type="OrthoDB" id="117507at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="PTHR11908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="Ald_Xan_dh_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="SSF54665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="SSF56003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ald_Xan_dh_C" evidence="1">
        <location>
            <begin position="18"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="763" mass="84098" checksum="87F176338BA5E1C5" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDV2</accession>
    <name>B7IDV2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1745</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102640.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1745"/>
    <dbReference type="EnsemblBacteria" id="ACJ76179">
        <property type="protein sequence ID" value="ACJ76179"/>
        <property type="gene ID" value="THA_1745"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1745"/>
    <dbReference type="eggNOG" id="ENOG502ZEI1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1260551_0_0_0"/>
    <dbReference type="OMA" id="LWISRFH"/>
    <dbReference type="OrthoDB" id="1990187at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="226" mass="26009" checksum="9360EFE780936F84" modified="2009-02-10" version="1">MRIIKNIIETLKMELGFIKVNNLKLEFSANTSENSINLSTKTLDKTLRTKVLRLEFENKVNVKNEVIEKNSIQIKDIAFSGNNLKLNELGFKKFSKVKNQTLAFKTPSCTKMPTVIKDIKIYKTNVFNIKVNKNYISSIYTITFSPTPSVKKEKILRLLKNIIANYKNSDIKFVGYYKNIPIGYAYNIIISDRKLILHAKSKGKDISKDIAVFKIGEKYKLEVIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDX4</accession>
    <name>B7IDX4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease R</fullName>
            <shortName evidence="5">RNase R</shortName>
            <ecNumber evidence="5">3.1.13.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rnr</name>
        <name evidence="7" type="ordered locus">THA_1770</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.13.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RNR ribonuclease family. RNase R subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.1" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580398.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1770"/>
    <dbReference type="EnsemblBacteria" id="ACJ76201">
        <property type="protein sequence ID" value="ACJ76201"/>
        <property type="gene ID" value="THA_1770"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1770"/>
    <dbReference type="eggNOG" id="COG0557">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002333_4_1_0"/>
    <dbReference type="OMA" id="DWYEYRS"/>
    <dbReference type="OrthoDB" id="1602988at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008859">
        <property type="term" value="F:exoribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01895">
        <property type="entry name" value="RNase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040476">
        <property type="entry name" value="CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013223">
        <property type="entry name" value="RNase_B_OB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004476">
        <property type="entry name" value="RNase_II/RNase_R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011805">
        <property type="entry name" value="RNase_R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17876">
        <property type="entry name" value="CSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08206">
        <property type="entry name" value="OB_RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00358">
        <property type="entry name" value="3_prime_RNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02063">
        <property type="entry name" value="RNase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="622"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="706" mass="80482" checksum="4FA885A3EDF97DCB" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDY3</accession>
    <name>B7IDY3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1779</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1779"/>
    <dbReference type="EnsemblBacteria" id="ACJ76210">
        <property type="protein sequence ID" value="ACJ76210"/>
        <property type="gene ID" value="THA_1779"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1779"/>
    <dbReference type="HOGENOM" id="CLU_1712412_0_0_0"/>
    <dbReference type="OMA" id="YFPFGTE"/>
    <dbReference type="OrthoDB" id="2034582at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="159" mass="17534" checksum="3AEC9F995BEC13FB" modified="2009-02-10" version="1">MFSIGAIFGGLFFLGVFYYIQNTSYVQIPSIEELKNLKVTEVSKILKKEGLQTEKFFGGGNLKLIIPESGSLVKRGRKIQIYGNQEINEGIFIPNLYGVHYLIGVDILKNLGLNVNLVKINYPGGDGRILASYPSFESTISNLEKINLLVDNGEIGGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE07</accession>
    <name>B7IE07_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidohydrolase family, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1803</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580427.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1803"/>
    <dbReference type="EnsemblBacteria" id="ACJ76234">
        <property type="protein sequence ID" value="ACJ76234"/>
        <property type="gene ID" value="THA_1803"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1803"/>
    <dbReference type="eggNOG" id="COG1574">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009942_3_1_0"/>
    <dbReference type="OMA" id="SVQPHFY"/>
    <dbReference type="OrthoDB" id="751534at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro_3" evidence="1">
        <location>
            <begin position="36"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="434" mass="50287" checksum="939F4FA3788A8EA6" modified="2009-02-10" version="1">MIKIKNAFVWDNGRFLKNDLYVKNGIFVDDCKNCAQEIDLEGKYILPGFSDSHAHVLGVGLSKLMLNLNSNNFQDEVFSSSEEIILGRGWTEINDKYIFDSITKPVILIRVCGHVAYLNKYAQKILGFNDYFIYENDLEKIWRLLPEEYYIKAFKTGEYEFISKGITSVHSDDLHGIDFETLLKLLNDSKLRIYEKLFAETPWNYEFNKEYGISKIFGIKLFADGSLGGKTAFLSRPYKNSKNYGTYTLPENFKLILDFAEKNNVQVCVHAIGDEAISRLIELFGRYNKHRIIHAQLIKEKDFEKLKNFKFSVQPHFYFEDQKILENIDTSGLMLYPFKRMFDEGYLISFSSDGPVSPIDPKYIISSALKLGFSFEDSIKLYTEYSGNMINEKIGLLKPNYKADFIVFKDKNLEKLEMVFINGEKVYDALTLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE45</accession>
    <name>B7IE45_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutamine synthetase, type I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">glnA2</name>
        <name evidence="4" type="ordered locus">THA_1841</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580456.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1841"/>
    <dbReference type="EnsemblBacteria" id="ACJ76272">
        <property type="protein sequence ID" value="ACJ76272"/>
        <property type="gene ID" value="THA_1841"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1841"/>
    <dbReference type="eggNOG" id="COG0174">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_1_2_0"/>
    <dbReference type="OMA" id="VFPWESK"/>
    <dbReference type="OrthoDB" id="416474at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_b-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="SSF54368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln-synt_C" evidence="3">
        <location>
            <begin position="101"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="456" mass="51932" checksum="8F6285B1363DCD41" modified="2009-02-10" version="1">MEIKEILELVEREKINLIDLKVVDILGKWRHVTLARTNFSEKTFYDGVGFDASNLGYAKVTNSDMIMIPDPSTAMIEYIGDDKVLSMICDVYDVETGEPSLHDPRSLLKKSLESISDIADKVYFGPEYEFHIFKDVRYTVESNDISVYIDSDEGFWNSFDKGEYFIGRKKGYHRIPPFDTLMEVRNKIVNELLKYGVPVKYHHHEVGTCQVEIELSFVDALKAADYTLLVKYIARKVAKEYGLIVTFMPKPLYNEAGNGMHVHQFMVKNEKNIFAGNKLYGLSEIALSYIAGLLKHAGSVMAFSNPSTNSYRRLVPGFEAPTNAVFALANRTAAIRIPAYVKDDKKRRIEFRTIDASCNPYLAFAAMILAGIDGIRKSLDPTQEGFGPFEGDAYNENIKPLPTSLDAACNELRKDNEYLTVFPKELINHWIRIKLSESREIMSIPHPKEFENYFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE58</accession>
    <name>B7IE58_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Purine nucleoside phosphorylase</fullName>
            <ecNumber evidence="3">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Inosine-guanosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1859</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102843.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1859"/>
    <dbReference type="EnsemblBacteria" id="ACJ76285">
        <property type="protein sequence ID" value="ACJ76285"/>
        <property type="gene ID" value="THA_1859"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1859"/>
    <dbReference type="eggNOG" id="COG0005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054456_1_0_0"/>
    <dbReference type="OMA" id="SCITDMA"/>
    <dbReference type="OrthoDB" id="1364220at2"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09009">
        <property type="entry name" value="PNP-EcPNPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011270">
        <property type="entry name" value="Pur_Nuc_Pase_Ino/Guo-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011268">
        <property type="entry name" value="Purine_phosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904">
        <property type="entry name" value="PTHR11904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000477">
        <property type="entry name" value="PurNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01700">
        <property type="entry name" value="PNPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01697">
        <property type="entry name" value="PNPH-PUNA-XAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="5">
        <location>
            <begin position="21"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate binding" evidence="4">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000477-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="266" mass="29745" checksum="84C9C2E674A5DAC6" modified="2009-02-10" version="1">MNEKIKETVDFIKNQIKILPDIGLILGSGLGFLAEKIENSTEISYKDIPNFPYSTVPGHEGKLVFGNLSGKNVVVLKGRFHLYEGWNPETIKFVIYTLRELGVERLLITNAAGGINRDFEPGDVVLIKDIINFQFRNPLRGSNDEYFGPRFPDMSSVVDFEWYEKVKKEFPNLKEGVYMAVLGPSYETPAEIRAFKRLGADLVGMSTVPEIIAAKHCGIKTLSFSCVTNMAAGILDTPLSHKEVVEVANRVKDTFSKIILKTLEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE66</accession>
    <name>B7IE66_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580465.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1867"/>
    <dbReference type="EnsemblBacteria" id="ACJ76293">
        <property type="protein sequence ID" value="ACJ76293"/>
        <property type="gene ID" value="THA_1867"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1867"/>
    <dbReference type="eggNOG" id="COG1315">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026157_1_0_0"/>
    <dbReference type="OMA" id="MEGFFPG"/>
    <dbReference type="OrthoDB" id="379544at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005646">
        <property type="entry name" value="FapA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38032">
        <property type="entry name" value="PTHR38032"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03961">
        <property type="entry name" value="FapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="456" mass="50915" checksum="3BB7CB8C64A105CC" modified="2009-02-10" version="1">MIIKVKVPENKMSASIILEKAGDDDKVTPKEIVDALNEAGVKFGIDYDVINRICQNPEFFVPLQVAVGKEPKNGEDGRIEFVNFEKEKESDKKNIDFREFPTHKRIIVRKGQKIATVYEPTEGEDGINVFGEKIAGKVGNKVDIKLGNNVELKDNSIYSKVDGILIVDLNENFIDVGEVLEIKGDVDYSIGNIDFPGEVVITGDVKPGFVVRAKKDIKVEGIIEAATVISLEGNIIVSGIKGRDKGIVKCSEDLNVRFAENANLEAKNLIFKDLLNNCNVKVSESIISENGRGVIVGGEYIAGVKIEAEEIGSEIGIPTRLEVGIPPHLLEERKLLHTQIMLDKQNAEKLMKIVKQYKLLKEKKVDIPNDKKQLFVKATTTLLNIKEQLEKNKKRLDEIEEMINKIKVDAKVVARKIIYPGVEIMLQGLKYYINKPLNKAVLRIENDNIIVGGYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEB6</accession>
    <name>B7IEB6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hydroxylamine reductase</fullName>
            <ecNumber evidence="6">1.7.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Hybrid-cluster protein</fullName>
            <shortName evidence="6">HCP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Prismane protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hcp</name>
        <name evidence="7" type="ordered locus">THA_1919</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>A + H2O + NH4(+) = AH2 + H(+) + hydroxylamine</text>
            <dbReference type="Rhea" id="RHEA:22052"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15429"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="1.7.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </cofactor>
        <text evidence="6">Binds 1 hybrid [4Fe-2O-2S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HCP family.</text>
    </comment>
    <dbReference type="EC" id="1.7.99.1" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580481.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1919"/>
    <dbReference type="EnsemblBacteria" id="ACJ76343">
        <property type="protein sequence ID" value="ACJ76343"/>
        <property type="gene ID" value="THA_1919"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1919"/>
    <dbReference type="eggNOG" id="COG1151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038344_2_0_0"/>
    <dbReference type="OMA" id="EINLKCM"/>
    <dbReference type="OrthoDB" id="337713at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050418">
        <property type="term" value="F:hydroxylamine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00069">
        <property type="entry name" value="Hydroxylam_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010048">
        <property type="entry name" value="Hydroxylam_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016100">
        <property type="entry name" value="Prismane_a-bundle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="PTHR30109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109:SF0">
        <property type="entry name" value="PTHR30109:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000076">
        <property type="entry name" value="HCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01703">
        <property type="entry name" value="hybrid_clust"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="3"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via persulfide group" evidence="6">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="495"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Cysteine persulfide" evidence="6">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="552" mass="61784" checksum="F310DAC22D2882A1" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEC2</accession>
    <name>B7IEC2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Beta-glucosidase</fullName>
            <ecNumber evidence="3 11">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">THA_1926</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3 11"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580486.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1926"/>
    <dbReference type="CAZy" id="GH1">
        <property type="family name" value="Glycoside Hydrolase Family 1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76349">
        <property type="protein sequence ID" value="ACJ76349"/>
        <property type="gene ID" value="THA_1926"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1926"/>
    <dbReference type="eggNOG" id="COG2723">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001859_1_3_0"/>
    <dbReference type="OMA" id="VEACDRK"/>
    <dbReference type="OrthoDB" id="654705at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102483">
        <property type="term" value="F:scopolin beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017736">
        <property type="entry name" value="Glyco_hydro_1_beta-glucosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="Glyco_hydro_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="PTHR10353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03356">
        <property type="entry name" value="BGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="401"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 10">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR617736-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR617736-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ76349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="441" mass="51818" checksum="B4434C9A0DAF8DAA" modified="2009-02-10" version="1">MERNDFPKDFIFGVATASYQIEGAYNEDGKVPSIWDVFSHTPGKIKNNENGDVACDHYHRYEEDIKIMKDIGVDAYRFSISWPRVMKNTKEKNEKGIDFYNKLIDKLLENNIIPFITIYHWDLPLFLYEKGGWLNDDIALYFQDYSSILFQNFGDRVKHWITLNEPWCSSFLGYFYGIHAPGHKNLQEAIKAAHNLLRAHGYSVEAFRDLVKDGKIGITNVTTKVEPADETEEDFYVVLLVDELTNGWFYDPIIFGRYPENARAILQRNGIDIFENDMDIISKKIDFFGINYYTRQLVKYAPEEPFMFKTIEGELEKTEMGWEIYPEGLYDMLLKIYNRYKTPLYITENGMAGPDKIENGKVHDTYRINYLKSHFENALKAIKDGVDLKGYFIWTLMDNFEWAEGYSKRFGIVYTDYTTQKRYLKDSAIWLKKFLEGEINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEE5</accession>
    <name>B7IEE5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76372">
        <property type="protein sequence ID" value="ACJ76372"/>
        <property type="gene ID" value="THA_1950"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1950"/>
    <dbReference type="HOGENOM" id="CLU_3206351_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="45" mass="5416" checksum="BFF1AD23E14EE79B" modified="2009-02-10" version="1">MQFQFLQGKVQTRASQNIGKAKEIHFNSYKVRYKLVETLQEMFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEE6</accession>
    <name>B7IEE6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1951</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76373">
        <property type="protein sequence ID" value="ACJ76373"/>
        <property type="gene ID" value="THA_1951"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1951"/>
    <dbReference type="HOGENOM" id="CLU_3141680_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="49" mass="5790" checksum="8DA8B4982AB87EBF" modified="2009-02-10" version="1">MGFLDSKFDIENIFQFLQGKVQTFFHSTPPRDIANFNSYKVRYKLSTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEF0</accession>
    <name>B7IEF0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endonuclease Cas1</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cas1</name>
        <name evidence="10" type="ordered locus">THA_1955</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas2 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580503.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1955"/>
    <dbReference type="EnsemblBacteria" id="ACJ76377">
        <property type="protein sequence ID" value="ACJ76377"/>
        <property type="gene ID" value="THA_1955"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1955"/>
    <dbReference type="eggNOG" id="COG1518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052779_2_0_0"/>
    <dbReference type="OMA" id="YYVGSFY"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09722">
        <property type="entry name" value="Cas1_I-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019858">
        <property type="entry name" value="CRISPR-assoc_Cas1_HMARI/TNEAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43219">
        <property type="entry name" value="PTHR43219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03641">
        <property type="entry name" value="cas1_HMARI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ76377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="331" mass="39272" checksum="6FDA9DF5A4B91C1E" modified="2009-02-10" version="1">MKETIYIFSSGTLKREDNSLVFITEDGQKKHIPVENLKEIFIFGEINLNKRLIEFLSKKEIMISFLNHYGFYVGSFYPREHYNSGFMILKQAQHYIDNEKRIYIAKKIVEGAAKNSLKILRYYKNRGRELDSHILEIEENIVKISKIEEIPRLMQVEGKIKKIYYESFGEIINSTDFKFTKRGKRPPIDRINALISFSNSLIYSIVLKEIYETHLDPRIGYLHSTNFRRFSLNLDIAEIFKPIIGDRTIFSVINKKIIGVKDFEKGLNRVFLSDKGKRKFIEALENRLNETVKLKKIGRNVSYRTLIRMELYKLEKHFLEDEVYEPLIMEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEG4</accession>
    <name>B7IEG4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alpha-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1970</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="6">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyl hydrolase 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580515.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1970"/>
    <dbReference type="CAZy" id="GH4">
        <property type="family name" value="Glycoside Hydrolase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76391">
        <property type="protein sequence ID" value="ACJ76391"/>
        <property type="gene ID" value="THA_1970"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1970"/>
    <dbReference type="eggNOG" id="COG1486">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045951_1_1_0"/>
    <dbReference type="OMA" id="YVPWYLH"/>
    <dbReference type="OrthoDB" id="277080at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001088">
        <property type="entry name" value="Glyco_hydro_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022616">
        <property type="entry name" value="Glyco_hydro_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32092">
        <property type="entry name" value="PTHR32092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02056">
        <property type="entry name" value="Glyco_hydro_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11975">
        <property type="entry name" value="Glyco_hydro_4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00732">
        <property type="entry name" value="GLHYDRLASE4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_4C" evidence="7">
        <location>
            <begin position="197"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="474" mass="54793" checksum="5D7B8799BA5D8179" modified="2009-02-10" version="1">MFKVAIVGAGSAVFSLRIISDFTKILEFKDVEICLMDIDESRLNSTYILANKLNEEMGAKLKFKTTTNLEEAIDGARFVINTAMAGGHDYLEKVRKIGEKWGYYRGIDSQEFNMVSDYYTISNFNQLDLFLKVARLVEEKGEKDAWLLQAANPVFEGTTLILRNVPINMVGFCHGHYGVHRLAEVIGLDISKLDWQVAGVNHGIWLNRFLYDGKDGYKVIDEWIGNNVYEPKHPFDDQLSLAAIDMYKFYGQMPIGDTVRNSSWKYNYDLETKKKWYGKFGGADSQIGWKWYQNNIKEITETINKLANFVSMNPNIKLLDYNTYKDYMNNEIFKEEIESIINPKKFSGEQHVPFIDSIVNNKKQRFVVNILNNGAIEGIDNDVAVEIPAWVDSSGIHPEQIMPKLSERVIKYYLKPRIMRMEMALEAFTKGDINVLKELLFRDPRTRSEEQVEGVLNEIMNLPENAKMREYYKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEU1</accession>
    <name>B7IEU1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-oxoglutarate synthase subunit KorB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_97</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579345.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_97"/>
    <dbReference type="EnsemblBacteria" id="ACJ74605">
        <property type="protein sequence ID" value="ACJ74605"/>
        <property type="gene ID" value="THA_97"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_97"/>
    <dbReference type="eggNOG" id="COG1013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048564_2_0_0"/>
    <dbReference type="OMA" id="FNNNIYG"/>
    <dbReference type="OrthoDB" id="981516at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="1">
        <location>
            <begin position="52"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="274" mass="30914" checksum="07B03D188203744C" modified="2009-02-10" version="1">MKRDDLINYLRIDRWPTVWCPGCGNGIILKSFIDAVNELKLEKDRVAVVSGIGCSSRATGYLDFNTLHTLHGRAIAFATGVKLARPDFKVVVMGGDGDITAIGGNHFIHACRRNLDITVIIFNNMIYGMTGGQHSPTTPHEKIASTMPYGNIENSFDIVELALTSGATYVARSTVYHYPLTVKYIKEGISHRGMSVIEVLTNCHTYYGRYNGMRNAWDIHEYFKNNTVMKEKAEKLSKEELEGKIIIGQFKKEEKPDFYENYKKIFSIVKNSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEU6</accession>
    <name>B7IEU6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_102"/>
    <dbReference type="EnsemblBacteria" id="ACJ74610">
        <property type="protein sequence ID" value="ACJ74610"/>
        <property type="gene ID" value="THA_102"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_102"/>
    <dbReference type="eggNOG" id="ENOG50348BT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_905749_0_0_0"/>
    <dbReference type="OMA" id="TCRVNSI"/>
    <dbReference type="OrthoDB" id="1723541at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032547">
        <property type="entry name" value="DUF4941"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16299">
        <property type="entry name" value="DUF4941"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="300" mass="34838" checksum="C777EF5D10E2CF6C" modified="2009-02-10" version="1">MKKFIVAIVIFLLALNIFSEIIKFDNYTLNATNINFETVEEILEKYSKFLSTSEISTGTIGSFNYIEWEDKIIYFSKNVVVLGENALDNPNFDDIFNFFNIKYLKKENEYNFAEMVITKVEDFGSYLQFDYLGKNFIDIEATDNAINIISKGLIYFKDNLYEPGSILLTKNIDSSYKTELNILPKRTIVQFIKEYNIKNIIVKLFGEKIEKYDSKSFAIIFKDSNINAVFVPNYSPDFSGNDWKVFSISDKFGKLVSKKFNLKLFYVPFVQVPKDLPAIVIFTSQNSWKQISEFVEGELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEY6</accession>
    <name>B7IEY6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PleD-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579371.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_142"/>
    <dbReference type="EnsemblBacteria" id="ACJ74650">
        <property type="protein sequence ID" value="ACJ74650"/>
        <property type="gene ID" value="THA_142"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_142"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1030283_0_0_0"/>
    <dbReference type="OMA" id="FADERMY"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="156"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="270" mass="31456" checksum="68D4001B1AB7ACA0" modified="2009-02-10" version="1">MEKISDVIFEASDTLKEKALFPLFSISVIDIIGTIEKKEFFYKMARTLFNIIPSVNAVEIFDSDKLIASFGTISGKNKDYKLDNIIFRLYYNKISQFEESLLKYILTIAEIHYRNVKKFEEVKENSLYDELTGALTRKAGIEILQKKFYEIKRTNKKASIVFIDIDGLKETNDRLGHFAGDKLLREFVLVIKELIRKGDFIIRWGGDEFVLFLNTMDAKCVINRLESLIKTKFSWGISIIPEGFNDILEAIEYADKLMYKQKFLKKMKNN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF14</accession>
    <name>DAPB_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-tetrahydrodipicolinate reductase</fullName>
            <shortName evidence="1">HTPA reductase</shortName>
            <ecNumber evidence="1">1.17.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapB</name>
        <name type="ordered locus">THA_173</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:35323"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:35331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579389.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IF14"/>
    <dbReference type="SMR" id="B7IF14"/>
    <dbReference type="STRING" id="484019.THA_173"/>
    <dbReference type="EnsemblBacteria" id="ACJ74678">
        <property type="protein sequence ID" value="ACJ74678"/>
        <property type="gene ID" value="THA_173"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_173"/>
    <dbReference type="eggNOG" id="COG0289">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047479_1_1_0"/>
    <dbReference type="OMA" id="ALKACEY"/>
    <dbReference type="OrthoDB" id="803114at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00018"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016726">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00102">
        <property type="entry name" value="DapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022663">
        <property type="entry name" value="DapB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022664">
        <property type="entry name" value="DapB_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="PTHR20836"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05173">
        <property type="entry name" value="DapB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000161">
        <property type="entry name" value="DHPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01298">
        <property type="entry name" value="DAPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000117377" description="4-hydroxy-tetrahydrodipicolinate reductase">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="71"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="95"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="217" mass="24366" checksum="796022E1F2C70A13" modified="2009-02-10" version="1">MRFGVVGFKGKMGKVILDVFSNDGHTPILLIDKDEIIEEDFPEVIVDFSTPDALEKTSSYCEKYNSNLVIGTTALTEKHFKILENLSKKVAVVQSYNFSIGINILLELLEKATKMLNDWDCEITEIHHSKKKDKPSGTAILLKNALNREVEIHSLRLGGIPGDHTVLFSNEGELIEIKHRAISRKVFALGALKAAKFILNKKSGFYTFKDIIKEAFI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-26" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF15</accession>
    <name>DAPH_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tetrahydrodipicolinate N-acetyltransferase</fullName>
            <shortName evidence="1">THP acetyltransferase</shortName>
            <shortName evidence="1">Tetrahydropicolinate acetylase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapH</name>
        <name type="ordered locus">THA_174</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of an acetyl group from acetyl-CoA to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + acetyl-CoA + H2O = CoA + L-2-acetamido-6-oxoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:13085"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58117"/>
            <dbReference type="EC" id="2.3.1.89"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (acetylase route): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family. DapH subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.89" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103652.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IF15"/>
    <dbReference type="SMR" id="B7IF15"/>
    <dbReference type="STRING" id="484019.THA_174"/>
    <dbReference type="EnsemblBacteria" id="ACJ74679">
        <property type="protein sequence ID" value="ACJ74679"/>
        <property type="gene ID" value="THA_174"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_174"/>
    <dbReference type="eggNOG" id="COG2171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103751_0_0_0"/>
    <dbReference type="OMA" id="HLEYDRR"/>
    <dbReference type="OrthoDB" id="752123at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00022"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047200">
        <property type="term" value="F:tetrahydrodipicolinate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01691">
        <property type="entry name" value="DapH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019873">
        <property type="entry name" value="DapH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013710">
        <property type="entry name" value="DapH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08503">
        <property type="entry name" value="DapH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03532">
        <property type="entry name" value="DapD_Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000376726" description="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01691"/>
        </source>
    </evidence>
    <sequence length="233" mass="24940" checksum="79D6B115F534CAFA" modified="2009-02-10" version="1">MNTQEIINLIANSKKKTLTVAYLTGNLKNIDFKSFEFFGTENFGILFGEYEEIVKLIENNKEHIEKYKLEIKARNSAIPLANLAKYNARIEPGAIIRDLVEIGDGAVIMMGAVINIGAKIGEGTMIDMNAVVGGRAIIGKNCHIGAGAVIAGVIEPPSAQPVIIEDNVMVGANAVILEGVRIGQNSVIAAGAVVIEDVPPNSVVAGVPAKIIKKVDEKTKQKTQIVEGLRKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF56</accession>
    <name>B7IF56_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103750.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_215"/>
    <dbReference type="EnsemblBacteria" id="ACJ74720">
        <property type="protein sequence ID" value="ACJ74720"/>
        <property type="gene ID" value="THA_215"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_215"/>
    <dbReference type="HOGENOM" id="CLU_1288369_0_0_0"/>
    <dbReference type="OMA" id="EMYLSAR"/>
    <dbReference type="OrthoDB" id="1630712at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="214" mass="24322" checksum="DC8274AC21509212" modified="2009-02-10" version="1">MKKVMIIALIVISLVSFSFTRYQERTFGIRINNPVDLKNDILPKPEFIDNLILYYGYQKNIDSQHNSPVSYGFGIYSNLDFDNEYIVRGASFDFAVKVFGVTLFNEGEKLQLGFQGEVFYKKEIFQKEAGSSSNLSDFGSVSFSHYNKIIVNSFTLKPGLRADFIFSKDVTFSGRVNYNIQFFMGAKTETGKEVVSDIEPMNSIDFDVALNIAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF74</accession>
    <name>B7IF74_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_235"/>
    <dbReference type="EnsemblBacteria" id="ACJ74738">
        <property type="protein sequence ID" value="ACJ74738"/>
        <property type="gene ID" value="THA_235"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_235"/>
    <dbReference type="HOGENOM" id="CLU_3318376_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="39" mass="4466" checksum="370E7425D38B9724" modified="2009-02-10" version="1">MKLIKERYEVEYFDYSNTGKVISTHVGIGMAGFGIEIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF83</accession>
    <name>B7IF83_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">L-allo-threonine aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_244</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine aldolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579439.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_244"/>
    <dbReference type="EnsemblBacteria" id="ACJ74747">
        <property type="protein sequence ID" value="ACJ74747"/>
        <property type="gene ID" value="THA_244"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_244"/>
    <dbReference type="eggNOG" id="COG2008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029381_0_4_0"/>
    <dbReference type="OMA" id="NKGGGAC"/>
    <dbReference type="OrthoDB" id="1124266at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023603">
        <property type="entry name" value="Low_specificity_L-TA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097:SF9">
        <property type="entry name" value="PTHR48097:SF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017617">
        <property type="entry name" value="Thr_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta_elim_lyase" evidence="5">
        <location>
            <begin position="5"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR017617-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="343" mass="38846" checksum="9395387EF6C0AABC" modified="2009-02-10" version="1">MEFIDLRSDTVTKPTDEMRKAMAYAEVGDDVYGDDPTVNKLEKLAAEILGKEAALFVPSGTFANQLALFTHCNRGDEVIIGDDYHIVQHEAGAAVIIAGVQLRTIETDRGALIPEVVEKKIRKVEDIHYPKTGLICLENAHSNGRVIRLENYEKIRNIAEKYKVPIHLDGARIFNAATFLKIQASEIAKYADSVSFCLSKGLCAPVGSLLVGNKEFIEKARKKRKIMGGGLRQAGILAAAGIIALEKMIFRLEEDHENAKYLAQKLLNFDFIEIVEDVEINMVFFKINKQFEEEDFVKYLFENGIKINPSDDGIFRFVTHYWIKKKDIDYVIEKMEKYFTAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFA4</accession>
    <name>B7IFA4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FeS assembly protein SufB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sufB</name>
        <name evidence="3" type="ordered locus">THA_267</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0051 (ycf24) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103837.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_267"/>
    <dbReference type="EnsemblBacteria" id="ACJ74768">
        <property type="protein sequence ID" value="ACJ74768"/>
        <property type="gene ID" value="THA_267"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_267"/>
    <dbReference type="eggNOG" id="COG0719">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026231_0_1_0"/>
    <dbReference type="OMA" id="YYSAPKQ"/>
    <dbReference type="OrthoDB" id="635721at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010231">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="SSF101960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01980">
        <property type="entry name" value="sufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SufBD_N" evidence="2">
        <location>
            <begin position="127"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="463" mass="52257" checksum="B1DF1511EBFDA25B" modified="2009-02-10" version="1">MFDIKQNEDRFNYIANIEPEYKSLPGLTPKIIEEISEIKGEPKWMRELRLKSLEIFQKWHDPRFGVDISELDLNKIIPYIKPKANKQTSWEEVPEEIKKAFDKLGIPEAERKYFAGVGAQFDSEIVYQNIKKELESLGVIFMDMESAVREYPDLVKEYFMKLVPAHDHKFAALHGAIWSGGTFLYVPKGVKVPMPLQAYFLMSNPGMSQLEHTVIVADEGSEVTFIEGCSAPRYNVINLHTGMVEIYVKKDAKVKYMTIQNWSKNTYNLNTKRSIVEENGVMSWVSGSLGSMKTMLYPMTILKGKGAKAESLGITYAGPGQHMDTGSKVVHLAPYTSSTIDARSISVGGGWAFYRGLLRIAQDAKKSKSHVQCTALMLDNKSKSDTVPLIEVYNSDSDVGHEARIGRIKDEQIFYLMTRGLSETEAKSMIVKGFIEPIVSSLPFEYALELNRLIEMEIESSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFC6</accession>
    <name>B7IFC6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyrazinamidase/nicotinamidase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_290"/>
    <dbReference type="EnsemblBacteria" id="ACJ74790">
        <property type="protein sequence ID" value="ACJ74790"/>
        <property type="gene ID" value="THA_290"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_290"/>
    <dbReference type="eggNOG" id="COG1335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_12_2_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="114" mass="13375" checksum="BE9502D27DE0CCAF" modified="2009-02-10" version="1">MKALMIIDMQNDFAKKGGALYFEGAEKVIPKIIDLIKEAKNKNLPIILTQDWHDEADKEFEIWPKHCIKNTAGAMIVSEIQELLNDYNNVYYIKKTRYSAFYKTDLENILEIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFJ3</accession>
    <name>B7IFJ3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BFN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579523.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_363"/>
    <dbReference type="EnsemblBacteria" id="ACJ74857">
        <property type="protein sequence ID" value="ACJ74857"/>
        <property type="gene ID" value="THA_363"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_363"/>
    <dbReference type="eggNOG" id="COG1259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096111_2_2_0"/>
    <dbReference type="OMA" id="LFKNFAD"/>
    <dbReference type="OrthoDB" id="1594801at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.690.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036104">
        <property type="entry name" value="BFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003729">
        <property type="entry name" value="Bi_nuclease_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02577">
        <property type="entry name" value="DUF151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103256">
        <property type="entry name" value="SSF103256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51658">
        <property type="entry name" value="BFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BFN" evidence="1">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="176" mass="20027" checksum="3243D98E3D9382B3" modified="2009-02-10" version="1">MKKATVKAVVLDKLNNAPVVILGIEGTNKILPIWIGACEASVLALMLENADFERPLTHDLMINIIEGLEAKVERVYINKIENNTFFARVILKDLTIPEEEEEGHYIEFDARPSDAIILALKTSSPIYVSNELVNTHTVNFEGEKFDDEDEEFKKFVENLDIEEFKRRFKKGSQGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFM7</accession>
    <name>B7IFM7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Peptidyl-tRNA hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_398</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_398"/>
    <dbReference type="EnsemblBacteria" id="ACJ74891">
        <property type="protein sequence ID" value="ACJ74891"/>
        <property type="gene ID" value="THA_398"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_398"/>
    <dbReference type="eggNOG" id="COG0193">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062456_4_1_0"/>
    <dbReference type="OMA" id="VICHSKG"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:aminoacyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00462">
        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018171">
        <property type="entry name" value="Pept_tRNA_hydro_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036416">
        <property type="entry name" value="Pept_tRNA_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224">
        <property type="entry name" value="PTHR17224"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53178">
        <property type="entry name" value="SSF53178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01196">
        <property type="entry name" value="PEPT_TRNA_HYDROL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="159" mass="18294" checksum="A211E4645EF7C56B" modified="2009-02-10" version="1">MFIDRVCNNFKKQNNYKYCQKTLFGKDILFVKPFTYMNLSGEIFRYLNKEDFSDIIVVYDDVNLPLGKIRIRKSGSSGGHNGIKSLISYLGEDFKRIRIGIGPIPENVNLVDFVLGEFEEDELKVLDKVLDLSIDALYTIFKDGIDKAMSLYNSQEVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFN1</accession>
    <name>B7IFN1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Adenylylsulfate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_402</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579550.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_402"/>
    <dbReference type="EnsemblBacteria" id="ACJ74895">
        <property type="protein sequence ID" value="ACJ74895"/>
        <property type="gene ID" value="THA_402"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_402"/>
    <dbReference type="eggNOG" id="COG0529">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1757952_0_0_0"/>
    <dbReference type="OMA" id="HKANECA"/>
    <dbReference type="OrthoDB" id="1937236at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="159" mass="18367" checksum="E712B7A511F7C833" modified="2009-02-10" version="1">MNNYIIWLVGKRKAGKSTIAKELNKYFIEKSLPSIVLEGRDIFKNVNIQVQERIKILANTAFLFSKLGYIVIVPAVSPKKSIREMIKKISPVPFFEIFITCDEKIRKKRIKSSDYFQLYVDKIFEEPVNPDLVIDTSLHKANECAKIIIDYVNKNIQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFN2</accession>
    <name>B7IFN2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">UvrABC system protein A</fullName>
            <shortName evidence="13">UvrA protein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Excinuclease ABC subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 16" type="primary">uvrA</name>
        <name evidence="16" type="ordered locus">THA_403</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Forms a heterotetramer with UvrB during the search for lesions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ABC transporter superfamily. UvrA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579551.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_403"/>
    <dbReference type="EnsemblBacteria" id="ACJ74896">
        <property type="protein sequence ID" value="ACJ74896"/>
        <property type="gene ID" value="THA_403"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_403"/>
    <dbReference type="eggNOG" id="COG0178">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001370_0_2_0"/>
    <dbReference type="OMA" id="PFEGIIP"/>
    <dbReference type="OrthoDB" id="152379at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00205">
        <property type="entry name" value="UvrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004602">
        <property type="entry name" value="UvrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041552">
        <property type="entry name" value="UvrA_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041102">
        <property type="entry name" value="UvrA_inter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17755">
        <property type="entry name" value="UvrA_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17760">
        <property type="entry name" value="UvrA_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00630">
        <property type="entry name" value="uvra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 13" id="KW-0228">DNA excision</keyword>
    <keyword evidence="12 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="2 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0742">SOS response</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="576"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="13">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="13">
        <location>
            <begin position="719"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="31"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <begin position="620"/>
            <end position="627"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ACJ74896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="924" mass="104004" checksum="CBDD8E8B2735E9A6" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFP5</accession>
    <name>RLMH_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA large subunit methyltransferase H</fullName>
            <ecNumber evidence="1">2.1.1.177</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">23S rRNA (pseudouridine1915-N3)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">23S rRNA m3Psi1915 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (pseudouridine-N3-)-methyltransferase RlmH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rlmH</name>
        <name type="ordered locus">THA_416</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>pseudouridine(1915) in 23S rRNA + S-adenosyl-L-methionine = H(+) + N(3)-methylpseudouridine(1915) in 23S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10221"/>
            <dbReference type="Rhea" id="RHEA-COMP:10222"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74486"/>
            <dbReference type="EC" id="2.1.1.177"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA methyltransferase RlmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.177" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579563.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFP5"/>
    <dbReference type="SMR" id="B7IFP5"/>
    <dbReference type="STRING" id="484019.THA_416"/>
    <dbReference type="EnsemblBacteria" id="ACJ74909">
        <property type="protein sequence ID" value="ACJ74909"/>
        <property type="gene ID" value="THA_416"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_416"/>
    <dbReference type="eggNOG" id="COG1576">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100552_2_0_0"/>
    <dbReference type="OMA" id="NEPYHHQ"/>
    <dbReference type="OrthoDB" id="1783583at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070038">
        <property type="term" value="F:rRNA (pseudouridine-N3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18081">
        <property type="entry name" value="RlmH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00658">
        <property type="entry name" value="23SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003742">
        <property type="entry name" value="RlmH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33603">
        <property type="entry name" value="PTHR33603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02590">
        <property type="entry name" value="SPOUT_MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004505">
        <property type="entry name" value="MT_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000131236" description="Ribosomal RNA large subunit methyltransferase H">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="120"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00658"/>
        </source>
    </evidence>
    <sequence length="152" mass="17721" checksum="52F706E67730D661" modified="2009-02-10" version="1">MNIEIIVPGKLSSHLQNVFDFYLKKLKRFANLSVTFTKLGGDVNRESKQVILRREKDEILNKVKNRRFILIDLHGKQMDSISFSKFVENEMLSGELLFVIGGPLGIDDELRKKASLKISLSKMTFTHEFTLVILLEQLFRAFKIINNEKYHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFQ3</accession>
    <name>B7IFQ3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-lysine 2,3-aminomutase</fullName>
            <ecNumber evidence="5">5.4.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">THA_424</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-lysine = (3S)-3,6-diaminohexanoate</text>
            <dbReference type="Rhea" id="RHEA:19177"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57434"/>
            <dbReference type="EC" id="5.4.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the radical SAM superfamily. KamA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.2" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104175.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_424"/>
    <dbReference type="EnsemblBacteria" id="ACJ74917">
        <property type="protein sequence ID" value="ACJ74917"/>
        <property type="gene ID" value="THA_424"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_424"/>
    <dbReference type="eggNOG" id="COG1509">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032161_0_0_0"/>
    <dbReference type="OMA" id="PIWLNTH"/>
    <dbReference type="OrthoDB" id="557227at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050066">
        <property type="term" value="F:lysine 2,3-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025895">
        <property type="entry name" value="LAM_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003739">
        <property type="entry name" value="Lys_aminomutase/Glu_NH3_mut"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022459">
        <property type="entry name" value="Lysine_aminomutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30538">
        <property type="entry name" value="PTHR30538"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12544">
        <property type="entry name" value="LAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004911">
        <property type="entry name" value="DUF160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00283">
        <property type="entry name" value="L-lysine_2_3-aminomutase_(LAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03820">
        <property type="entry name" value="lys_2_3_AblA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00238">
        <property type="entry name" value="TIGR00238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="15">
        <location>
            <begin position="112"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="14">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR004911-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR603739-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ACJ74917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="423" mass="49005" checksum="D8846A456D5BB6A3" modified="2009-02-10" version="1">MRNYKEIPLWKNVSEEEWNDWKWQIRNRITDVDTLKQVINLTPEEENGIRNSLKTLRMAITPYYASLMDPDNPKCPIRRQAVPTIKELEVKPWDMVDPLHEDEDSPVPGLTHRYPDRVLLLVTDMCAMYCRHCTRRRFAGQHDRARTKQEIDAAIEYIRETPQVRDVLLSGGDALLAGIDMLEYILKELRKIKHVEIIRIGSRAPVVIPQIVTKELTDMLKKYHPIWLNTHFNHPKEITPESSRACEMLADAGIPLGNQSVLLRGVNDSPYIMMELVHQLVKIRVRPYYLYQCDLSQGISHFRTSIGTGLRIIESLIGHTSGFCVPTYVVDAPAGGGKIRLMPEYLISYSDKTAILRNYEGVIVAYHEPEDTESDVDDSEYREKYKLSGIASLYDSKKISMEPAHLERHERIRKWKEKRGEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFS3</accession>
    <name>B7IFS3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NusG_II domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579586.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_446"/>
    <dbReference type="EnsemblBacteria" id="ACJ74937">
        <property type="protein sequence ID" value="ACJ74937"/>
        <property type="gene ID" value="THA_446"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_446"/>
    <dbReference type="eggNOG" id="COG5341">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130936_3_1_0"/>
    <dbReference type="OMA" id="CPDKICE"/>
    <dbReference type="OrthoDB" id="1864717at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038690">
        <property type="entry name" value="NusG_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024045">
        <property type="entry name" value="NusG_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07009">
        <property type="entry name" value="NusG_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NusG_II" evidence="1">
        <location>
            <begin position="12"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="115" mass="13100" checksum="843EFDB70ADBE7D1" modified="2009-02-10" version="1">MKAFKKIDVFLILAVILITVFFALKANSREDEIFLVKLDGKIYTELKKPGDYPVKDKEGKILTIVHYDGENVWVTDSTCPLKICEKTGKIKRGGKIICVPNKIVIESQTQELQTW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFV5</accession>
    <name>SSRP_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SsrA-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Small protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">smpB</name>
        <name type="ordered locus">THA_478</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">The tmRNA-SmpB complex associates with stalled 70S ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SmpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104260.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFV5"/>
    <dbReference type="SMR" id="B7IFV5"/>
    <dbReference type="STRING" id="484019.THA_478"/>
    <dbReference type="EnsemblBacteria" id="ACJ74969">
        <property type="protein sequence ID" value="ACJ74969"/>
        <property type="gene ID" value="THA_478"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_478"/>
    <dbReference type="eggNOG" id="COG0691">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108953_0_0_0"/>
    <dbReference type="OMA" id="WTNHSAR"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070929">
        <property type="term" value="P:trans-translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09294">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00023">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023620">
        <property type="entry name" value="SmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000037">
        <property type="entry name" value="SsrA-bd_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020081">
        <property type="entry name" value="SsrA-bd_prot_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30308">
        <property type="entry name" value="PTHR30308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01668">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74982">
        <property type="entry name" value="SSF74982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00086">
        <property type="entry name" value="smpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01317">
        <property type="entry name" value="SSRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_1000116428" description="SsrA-binding protein">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00023"/>
        </source>
    </evidence>
    <sequence length="149" mass="17496" checksum="E817ADB1B5CAE17C" modified="2009-02-10" version="1">MKVIATNKKAYSDYNILETYEAGIELRGTEVKALRESGANFKDSFCRIKNGEVFLLNLNIPQYRNGNLNNHDPERPRRLLLHKKEIHRLIGKVKEQGLTIIPTKIYFNSRGLVKVEIAVAKGKKKYDKREDIKKREINRKINEYLKRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG04</accession>
    <name>B7IG04_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ggdef domain/hd domain protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579633.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_530"/>
    <dbReference type="EnsemblBacteria" id="ACJ75018">
        <property type="protein sequence ID" value="ACJ75018"/>
        <property type="gene ID" value="THA_530"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_530"/>
    <dbReference type="eggNOG" id="COG3437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_936689_0_0_0"/>
    <dbReference type="OMA" id="NDEWNDY"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD-GYP" evidence="1">
        <location>
            <begin position="147"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="312" mass="35646" checksum="F3E6C6D762EEDBAD" modified="2009-02-10" version="1">MFEFKNLVVDSFGKAGKKVILKLDIKDHTIEIYDALPDYETLYFIANFFEIFDPKFSENIISKIINSNNLDDVLNAMKKTWDLDDVLYDENDFEIEFSNNTFYFPIYSKTAGRLGTIILKGNLSSEFILNFLAISDAITSILEGFILKMRVSELLYSTLEALSKALTKKINVDEKLQKEIENKIIAIGEKFNIPKDILKIASKIYDLGKIGVPDYVFSKKELSKEDLEILNKHVEYGYEILSKIEDIPDQILKAVLYHHEKKDGSGPLKVKNVPLLAQIIGIVLEVVENKTPIDALQGKYDEKLIEEMKKNG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG18</accession>
    <name>Y544_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0173 metal-dependent hydrolase THA_544</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">THA_544</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0173 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579640.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IG18"/>
    <dbReference type="SMR" id="B7IG18"/>
    <dbReference type="STRING" id="484019.THA_544"/>
    <dbReference type="EnsemblBacteria" id="ACJ75032">
        <property type="protein sequence ID" value="ACJ75032"/>
        <property type="gene ID" value="THA_544"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_544"/>
    <dbReference type="eggNOG" id="COG2220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070010_4_0_0"/>
    <dbReference type="OMA" id="WHGHSTW"/>
    <dbReference type="OrthoDB" id="767622at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00457">
        <property type="entry name" value="UPF0173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022877">
        <property type="entry name" value="UPF0173"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000197818" description="UPF0173 metal-dependent hydrolase THA_544">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00457"/>
        </source>
    </evidence>
    <sequence length="223" mass="24570" checksum="ED6212CC378C05CE" modified="2009-02-10" version="1">MKITFLGHAVFLIETNKKILIDPFITGNPAFPKDFSFDKIDYILVTHGHGDHIGDTVELSKKYNATVVSNFEICNYLQKKGVNKVHPMHIGGSFNFDFGKLKMTPALHGSGIIEGDNIIYGGNPGGFVIYSEKSVYHAGDTGLTKDMELLRGVDVAILPIGGNFVMDVEDALKAVEMIKPKVVIPMHYNTWDIISADEEKFKKGSKQLGVKCIILKPGESVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG25</accession>
    <name>B7IG25_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100594.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_551"/>
    <dbReference type="EnsemblBacteria" id="ACJ75039">
        <property type="protein sequence ID" value="ACJ75039"/>
        <property type="gene ID" value="THA_551"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_551"/>
    <dbReference type="HOGENOM" id="CLU_1453751_0_0_0"/>
    <dbReference type="OMA" id="VFISSMF"/>
    <dbReference type="OrthoDB" id="2142311at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002854519" evidence="1">
        <location>
            <begin position="20"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="186" mass="21799" checksum="7EECD64F8EAD6A1E" modified="2009-02-10" version="1">MRKLVFLLVSIVFISALFAFPYNYQTAKPMDYENVWDKLRNVEVSEPATYTGTIKEVYIIANPGFSKSKIILETDEGSDVEIFVGPMWRFFEFKPGEKVELEVVKVNFSKDSSFYLAYKLRSEGITVEIPYKQMIKERIENLKRLKFQNNVVPGFQRRPFRPMMPHYGNPYGNGYPPMNRPQNGWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG28</accession>
    <name>B7IG28_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA helicase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_554</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579644.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_554"/>
    <dbReference type="EnsemblBacteria" id="ACJ75042">
        <property type="protein sequence ID" value="ACJ75042"/>
        <property type="gene ID" value="THA_554"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_554"/>
    <dbReference type="eggNOG" id="COG1112">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1643">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001666_8_2_0"/>
    <dbReference type="OMA" id="YANDITF"/>
    <dbReference type="OrthoDB" id="616258at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004483">
        <property type="entry name" value="SMUBP-2/Hcs1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="PTHR10887"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00376">
        <property type="entry name" value="TIGR00376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="166"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="327"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="639" mass="73831" checksum="BF5C155F28892591" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG51</accession>
    <name>B7IG51_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_578</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100545.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_578"/>
    <dbReference type="EnsemblBacteria" id="ACJ75065">
        <property type="protein sequence ID" value="ACJ75065"/>
        <property type="gene ID" value="THA_578"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_578"/>
    <dbReference type="eggNOG" id="ENOG5032ZFW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1029540_0_0_0"/>
    <dbReference type="OMA" id="YHRHVAV"/>
    <dbReference type="OrthoDB" id="1323068at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032606">
        <property type="entry name" value="DUF4895"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16236">
        <property type="entry name" value="DUF4895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="264" mass="30702" checksum="A9AE3CB25798692A" modified="2009-02-10" version="1">MVYFGPEKLSYIGKKLENILSSDKEKLLDFLNSYKEKLDVFHKHLILVTVSSDNDFALSAGFNPEGKFFYGISITSPFLKSPSLYKIKEYVNEEFEALYNTIFNKSTISKVQAGIIRFPLQTHFLAVGGEKSLVSKEMFSEEITGKPWLNFAKIIDNEAYEKIMNINGKKIGFLKMSLTDDGIYFFGIINDPHKNLYAEFFRFLKEKYKLPSGKYYPVMEIKDKVVGTFKLELEEIFSEEKWKILEKFIDDFEKFRRFVAQMGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG53</accession>
    <name>B7IG53_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoate protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579658.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_580"/>
    <dbReference type="EnsemblBacteria" id="ACJ75067">
        <property type="protein sequence ID" value="ACJ75067"/>
        <property type="gene ID" value="THA_580"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_580"/>
    <dbReference type="eggNOG" id="COG0095">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022986_5_0_0"/>
    <dbReference type="OMA" id="HQGDLTY"/>
    <dbReference type="OrthoDB" id="871298at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="23"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="238" mass="27117" checksum="D85A1BE70D8A2A5D" modified="2009-02-10" version="1">MFYIETWKLPGDLNMAIDYVLGKSQSSYFRLYTWKNPTLSLGKNQSVDVVNTTYLKENGIDCVKRPTGGRAVLHSKELTYSFIIPNTDPLYRLSVLKLYKEISTIIVEALNNIGISAEIVSHSSRGNTQLCFDAPSWYEIVLNGKKIIGSAQMRTKSFVLQHGSIVLKTTAGIEKCFKDQSEPITQYGLDLAKEVDIEELKNSLYKSFSKRFQLKRFENVNEIISLAENERMKFKCCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG55</accession>
    <name>B7IG55_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_582</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100538.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_582"/>
    <dbReference type="EnsemblBacteria" id="ACJ75069">
        <property type="protein sequence ID" value="ACJ75069"/>
        <property type="gene ID" value="THA_582"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_582"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_6_1_0"/>
    <dbReference type="OMA" id="IWQKERG"/>
    <dbReference type="OrthoDB" id="2034748at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="65" mass="7290" checksum="D0F66D1E425051E6" modified="2009-02-10" version="1">MKGTVKWFDAKKGYGFITKEDGEDIFVHWSAIQTDGFKTLKEGQEVEFDVQDGQKGPQAANVKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG63</accession>
    <name>B7IG63_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pleiotropic regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_590</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579665.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_590"/>
    <dbReference type="EnsemblBacteria" id="ACJ75077">
        <property type="protein sequence ID" value="ACJ75077"/>
        <property type="gene ID" value="THA_590"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_590"/>
    <dbReference type="eggNOG" id="COG0399">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033332_6_0_0"/>
    <dbReference type="OMA" id="IVNHGMY"/>
    <dbReference type="OrthoDB" id="1006364at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="PTHR30244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="380" mass="43260" checksum="ABF2371C6B22996E" modified="2009-02-10" version="1">MIPLFDITRQYSKIKSEIIEAIDNVLSNGRVILGQEVERLEQNIANFIGVKFGIGVANGSDALVIALKAMEIKEGEKVITTPYTFFATASAIVRNGGIPIFVDVDSETYNIDLNQVEDILKKEKVFGIIPVHLFGQTVDLEGLYYLKEKYGIKILEDCAQSIGSEGILNGQVKKSGSIGDAAIFSFFPTKNLGAYGDGGMIVTDDERIYEKAKMLRVHGAKKKYFHEEIGFNSRLDEIQAAILNVKFKYLNSWTENRIKIAKKYKEEFEKHKLEVIYPKILNKTYKYHVFHQYVVKFKSNKIREKVREYLSENKVGTSLYYPVPLHLQKCFAYLGYKEGNLPVSEELSKTTLALPIFPELEEKEIEIIVQKIKEAIEEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG72</accession>
    <name>B7IG72_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104379.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_599"/>
    <dbReference type="EnsemblBacteria" id="ACJ75086">
        <property type="protein sequence ID" value="ACJ75086"/>
        <property type="gene ID" value="THA_599"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_599"/>
    <dbReference type="eggNOG" id="ENOG5033H0K">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182114_0_0_0"/>
    <dbReference type="OrthoDB" id="1955109at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="86" mass="10515" checksum="4B7E86B013F1147B" modified="2009-02-10" version="1">MNFIWDYCFGTETIIRKMVLGNLEDFSELLNKYKEDELKEIFLSNLHRFNGKDRSFWKVILEVSNEEIRRKSKESFRKDFALRNFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG76</accession>
    <name>B7IG76_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_604</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579676.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75090">
        <property type="protein sequence ID" value="ACJ75090"/>
        <property type="gene ID" value="THA_604"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_604"/>
    <dbReference type="eggNOG" id="COG4118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181375_0_0_0"/>
    <dbReference type="OrthoDB" id="2066870at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="93" mass="10647" checksum="2436CC8FDA04BBC6" modified="2009-02-10" version="1">MSKIRYVRSREIRSNPAILWKENETIITSNGKPKAIVIRIENDIESTLAAFRQVKAMRAVEKMRLISKQKGLDKISDEEIDKVIDEEQGEGSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG87</accession>
    <name>B7IG87_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">sn-glycerol-3-phosphate transport system permease protein UgpE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_615</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (UgpC), two transmembrane proteins (UgpA and UgpE) and a solute-binding protein (UgpB).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579683.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_615"/>
    <dbReference type="EnsemblBacteria" id="ACJ75101">
        <property type="protein sequence ID" value="ACJ75101"/>
        <property type="gene ID" value="THA_615"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_615"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_1_0"/>
    <dbReference type="OMA" id="FNSMAAY"/>
    <dbReference type="OrthoDB" id="1074175at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015794">
        <property type="term" value="P:glycerol-3-phosphate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001407">
        <property type="term" value="P:glycerophosphodiester transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030165">
        <property type="entry name" value="UgpE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43744:SF8">
        <property type="entry name" value="PTHR43744:SF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="71"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="277" mass="30971" checksum="822C7FB7DB2ABE27" modified="2009-02-10" version="1">MNRKNKVSFIISEIILILVTIIMFSPIFLAFMMSFMTPAEVFSFPPKIIPKSLYWQNYKEALRLVPLGRMIINSAIVALFITLGKLITGILAGYAFANFNFKGKKVAFATLFITLFLPAETVMIVPLFLLMKSLGWVNTYYALIIPFTASATNTFLMRQHFRTIPMELSDAARIDGASPMQYLFKVLLPLSKAMIGGAALINFVYAWNMYLWPLVVTMDDKMKTAQIGVKMLIDAEAANNWGTIMAGTMIVLAPTLLVFFLIQNLFVKSLVSSGLKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGA5</accession>
    <name>RS6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsF</name>
        <name type="ordered locus">THA_639</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds together with S18 to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104467.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IGA5"/>
    <dbReference type="SMR" id="B7IGA5"/>
    <dbReference type="STRING" id="484019.THA_639"/>
    <dbReference type="EnsemblBacteria" id="ACJ75119">
        <property type="protein sequence ID" value="ACJ75119"/>
        <property type="gene ID" value="THA_639"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_639"/>
    <dbReference type="eggNOG" id="COG0360">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113441_5_0_0"/>
    <dbReference type="OMA" id="ETIFLVK"/>
    <dbReference type="OrthoDB" id="1776954at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00473">
        <property type="entry name" value="bS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00360">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_S6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020814">
        <property type="entry name" value="Ribosomal_S6_plastid/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_S6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribosomal_S6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="PTHR21011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="SSF54995"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00166">
        <property type="entry name" value="S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000120815" description="30S ribosomal protein S6">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00360"/>
        </source>
    </evidence>
    <sequence length="119" mass="14445" checksum="43D72B4EA8864D2A" modified="2009-02-10" version="1">MRIYETMFIIKPDIAEEEREKIANGVVEFLKEKLNAQIDNVDRWGIRKTAYPLKKYNEADYTVVYFRATGENLNELEMYFKVRPEFLRWQTFRRIDLEKKERKTAKKVETVENTEKVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGB3</accession>
    <name>B7IGB3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Inosine-5-monophosphate dehydrogenase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_649</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579700.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_649"/>
    <dbReference type="EnsemblBacteria" id="ACJ75127">
        <property type="protein sequence ID" value="ACJ75127"/>
        <property type="gene ID" value="THA_649"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_649"/>
    <dbReference type="eggNOG" id="COG2172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_926298_0_0_0"/>
    <dbReference type="OMA" id="YELEMNL"/>
    <dbReference type="OrthoDB" id="1832160at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="20"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="83"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="306" mass="34715" checksum="28585AC96F46A683" modified="2009-02-10" version="1">MDLFDKVKHIFSDMKVSEFMNSDVIYVLPNRTIAQVKEILRLKRISGVPVVNYKKRVVGIISIEDIIKCLEANSLNALVEEKMTKNVVVVNVNDTLRDVMELFEKYGYGRFPVVDDEHRLVGIVTKNDILKSVAMKLGIMYLHDERRERALQNSEKSLILGMDLKKEESDFYFKIDYYDINQIGIGAASLKKFLLSKGVDEKLVRKIAISVYEAEANVVIHSGSEGEIYCFIKDDSIVVRVEDKGKGIENLDLAMKEGFSTAPDHVRELGFGAGMGLPNMKRYSDKLVILSEKDKGVIVEMVFFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGC1</accession>
    <name>B7IGC1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signaling protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_658</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579706.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_658"/>
    <dbReference type="EnsemblBacteria" id="ACJ75135">
        <property type="protein sequence ID" value="ACJ75135"/>
        <property type="gene ID" value="THA_658"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_658"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038242_0_0_0"/>
    <dbReference type="OMA" id="SETANIW"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="383"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="495" mass="58172" checksum="BB7581CC21C93ECF" modified="2009-02-10" version="1">MKKIVKNLIIALFTFVVLGILINGNFVKDKYVLNNWNQKVPDQILLDEPSTCVYVTEFNDTSFDTLVIPKIIGNLFKVYLNGEEIYSYGTKTGNIWSYTYVVPLKNLSSNKNILKIEIFGLYDAGLPRFPFLTDRISALIYQNFQNFLRHDFYIISIGISFVAGIISFFLGYLLKSKQISQSYDLFGLFLILTAIALFDYQVRTFHFNEITFLIFKKLFLISTYFSSLFYIAALEKYKLKRFRTKWLIWYVFFVSLLPVFSFDFNNYAKFSTVSNMLMLIVLLFVIYDVLYLKMSKLYFSTFFIVFSYIHIILYFLYVRSYHFQGFLTGYGTAFLSISVILMLIDEFEKMVAETNELSNSRYIDPLTKAYNRNILEKLDNSNVEGFFVLIDLDNFKELNDRFGHEKGDEILKKFSNLIIENIRKEDLFIRLGGDEFALIVNLDDPKSFVERLRKLSINVINLDFSYGIVKFSKFSESYRLADKLLYDMKSRNKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGD8</accession>
    <name>B7IGD8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Anaerobic ribonucleoside-triphosphate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nrdD</name>
        <name evidence="1" type="ordered locus">THA_677</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579721.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_677"/>
    <dbReference type="EnsemblBacteria" id="ACJ75152">
        <property type="protein sequence ID" value="ACJ75152"/>
        <property type="gene ID" value="THA_677"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_677"/>
    <dbReference type="eggNOG" id="COG1328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019682_0_0_0"/>
    <dbReference type="OMA" id="TNAGHIT"/>
    <dbReference type="OrthoDB" id="195920at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008998">
        <property type="term" value="F:ribonucleoside-triphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012833">
        <property type="entry name" value="NrdD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13597">
        <property type="entry name" value="NRDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02827">
        <property type="entry name" value="RNR_anaer_Bdell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="651" mass="75387" checksum="5DC9DD31A7E9FFAB" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGF8</accession>
    <name>B7IGF8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptide methionine sulfoxide reductase MsrA</fullName>
            <shortName evidence="6">Protein-methionine-S-oxide reductase</shortName>
            <ecNumber evidence="6">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Peptide-methionine (S)-S-oxide reductase</fullName>
            <shortName evidence="6">Peptide Met(O) reductase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">msrA</name>
        <name evidence="8" type="ordered locus">THA_701</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>[thioredoxin]-disulfide + H2O + L-methionine = [thioredoxin]-dithiol + L-methionine (S)-S-oxide</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[thioredoxin]-disulfide + H2O + L-methionyl-[protein] = [thioredoxin]-dithiol + L-methionyl-(R)-S-oxide-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 6">
            <text>[thioredoxin]-disulfide + H2O + L-methionyl-[protein] = [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein]</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579738.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_701"/>
    <dbReference type="EnsemblBacteria" id="ACJ75172">
        <property type="protein sequence ID" value="ACJ75172"/>
        <property type="gene ID" value="THA_701"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_701"/>
    <dbReference type="eggNOG" id="COG0225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0229">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_7_1_0"/>
    <dbReference type="OMA" id="FWGVEYH"/>
    <dbReference type="OrthoDB" id="1554384at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033744">
        <property type="term" value="F:L-methionine:thioredoxin-disulfide S-oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="SSF51316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="SSF55068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00357">
        <property type="entry name" value="TIGR00357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MsrB" evidence="7">
        <location>
            <begin position="2"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="292" mass="33647" checksum="ADC01A14B7830C14" modified="2009-02-10" version="1">MAKKINKNLSEFEKFVLFEKGTERPFSGKFEDHFEKGIYVCKNCGIPLYNSSSKFNSGCGWPAFDDEIPGAIKKQVDRDGIREEIVCSYCGAHLGHVFYGEGFTEKNVRHCVNSVSLDFVPEGEKPLIDRIFFAGGCFWGVEYLFRKLDGVVDTRVGYMGGRRKNPTYEQVCTGVTGHLETVEVIFDPKKLDEETLIKYFFEIHDFTQENGQGPDIGEQYKSAIFYTNDLQKKISEKLIQALKKKYDVVTQLKKASDFWVAEDYHQQYYEKTGKTPYCHVRFKLLKTWDGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGK1</accession>
    <name>B7IGK1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_749"/>
    <dbReference type="EnsemblBacteria" id="ACJ75215">
        <property type="protein sequence ID" value="ACJ75215"/>
        <property type="gene ID" value="THA_749"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_749"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126015_0_0_0"/>
    <dbReference type="OMA" id="QLMEECK"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="165" mass="19547" checksum="B17109A71FAB57B2" modified="2009-02-10" version="1">MEDNKIKLLEIIYENPGITQREIAKRTGLSLGLVNLLLKKFVRKGLIKLEKLNKKTFKYILTPKGFAEKAKKTIQYIRIYYEKIKQIKVKVEKIVKENGKDKIYAIYGEDEFIINVVREVLKNLKVKYVIYDKTDEITQTQDMIILYWDVEKSNILKDLRSFLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGK5</accession>
    <name>B7IGK5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Macrolide-efflux determinant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_753</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579778.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_753"/>
    <dbReference type="EnsemblBacteria" id="ACJ75219">
        <property type="protein sequence ID" value="ACJ75219"/>
        <property type="gene ID" value="THA_753"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_753"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_16_3_0"/>
    <dbReference type="OMA" id="VQVWHVY"/>
    <dbReference type="OrthoDB" id="498436at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="14"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="430" mass="47658" checksum="41CF9590917EEB65" modified="2009-02-10" version="1">MRRTDSTNKLFTKNFLLYIVGRLVSLIGSGIQMIAIPLYILDVTGSGAAMGIFTLLSILPRLSVAPFAGVLGDRFNRKNIMVLTDFIRGFLILFLALLSYTNSLSILILFIIQAFVAMFDGFFGAATTAMIPDIVSEDKLRSANSVLGSVNSFSMIIGPILGGIIYGLFGIFAVFLINGTSFVLSAISEMFIVYKVKFSKKSKLNVSSFFSEFKEGLTFIFKNDGLKYLFTFAMIINFLMSPLFQVVEPYVLRQIVKMSAQQYGFVQTFFTIGMLAGNVFLMSFLKNAKNKTLMISGVSIQSLAIFAFTILIFPNILVKFSIWNFFWLTSIIYFIVGAFNTLVNIPISTNLQLMTPSEIRSRVFSTLEIFSQIMVPLGAVIYGFLIDVVAAHILFLIVNFLALAISIIFFVISPQQVYEPKAGGDFDAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGL9</accession>
    <name>B7IGL9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type dipeptide/oligopeptide/nickel transport systems, permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_770</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100322.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_770"/>
    <dbReference type="EnsemblBacteria" id="ACJ75233">
        <property type="protein sequence ID" value="ACJ75233"/>
        <property type="gene ID" value="THA_770"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_770"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_0_0"/>
    <dbReference type="OMA" id="ETVFGRN"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="139"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="101"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="329" mass="37528" checksum="97A3A4BEAEFD6590" modified="2009-02-10" version="1">MKKYLRNKIIIYILTFIFAVTIDWLIPRLMPGNPILVLVSRFSGLPDAAKVMYSYLTKAFGLDQPLWTQYVNFWIAFFKGDLGISIYLYPKPVLDVLKSALPYSLGLLIPSILISWIIGNNLGAFAARRKKFDSFMLPVMYFLTGAPYLWLGILLAYFFGVVLRWVPIAGAYSFSLRPHFSWTFIADFLKHWILPFLSLFIVQLGGWAIGMRNMVIYELENNYVRFLETMGVKRSLIKKYTFRNAILPQVTGLALQLGTVIAGQVATEVVFSYPGVGYILTQAILNQDYFLIQGCFLFIIIGVLVANFTVDLVYMILDPRIRYSYGGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGM5</accession>
    <name>B7IGM5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, LacI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_776</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579791.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_776"/>
    <dbReference type="EnsemblBacteria" id="ACJ75239">
        <property type="protein sequence ID" value="ACJ75239"/>
        <property type="gene ID" value="THA_776"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_776"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_0"/>
    <dbReference type="OMA" id="CVAVDFR"/>
    <dbReference type="OrthoDB" id="664909at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00036">
        <property type="entry name" value="HTHLACI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="334" mass="37813" checksum="FE4CF133031BA26F" modified="2009-02-10" version="1">MVTIKDVAEHAGVSIATVSRVINGAKNVSDDTRKKVIKVIKKLGYKPMPSLRNRSELFYTLGVMVPDLKGYHYNEILMAIEEYASVHGFEIMVSVPKMSPEEEKHVLDQYFKRKIDGIILCELFGDTDYLEPFINSGVPIVALDYYIEEILCDSVNIDNVSGARSAMKYLYNMGHRNILYIRGVEKSPASKNREKGIEKFLRKHKDVQVYFSEHSGYNPSDGYEAVESFLKKHGLKFTAIFAVNDWASIGALRALYDHGLRVPDDVSIIGYDNGPFSEYLYPPLTTIHQPRREMGLTAAQLLIERILGKGPKIPRNVVLPTKLIERKSVKKVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGN3</accession>
    <name>B7IGN3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Fe-hydrogenase gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_785</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="6">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100354.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_785"/>
    <dbReference type="EnsemblBacteria" id="ACJ75247">
        <property type="protein sequence ID" value="ACJ75247"/>
        <property type="gene ID" value="THA_785"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_785"/>
    <dbReference type="eggNOG" id="COG1905">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054362_2_1_0"/>
    <dbReference type="OMA" id="MYDTKPV"/>
    <dbReference type="OrthoDB" id="1206880at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="157" mass="17573" checksum="B39C5EB7E01AFC4F" modified="2009-02-10" version="1">MERNFEKVEEILKKHGYEKKNLIKILLDVQKEYRHIPKEVVNYLSVALDIPPAKIFGVATFYAQFSLKPKGEYTILICDGTACHMEGSMSLIKAIEEEVGVKPGEVTQDLKFSLDKVGCLGACALAPAMVINGEVYGNLTPEKTKEILRKLKEGDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGU0</accession>
    <name>B7IGU0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase, group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579826.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_843"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75304">
        <property type="protein sequence ID" value="ACJ75304"/>
        <property type="gene ID" value="THA_843"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_843"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045353_0_0_0"/>
    <dbReference type="OMA" id="FDPWKDL"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="207"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="404" mass="46035" checksum="B7600FB507D62D76" modified="2009-02-10" version="1">MRVELPVRKISDYNTVAKEEVEELIELSKELKGLKVVHVNATSYGGGVAELLYTLAPLMNDLGLNTSWEVLEAPNEFFNVTKKLHNAFQGADIDISDNEFELFEKVNKDNAKKLNLDADAVIIHDPQPFFIPLFKDGKYIWRCHIDTSNPNFNVWNRITYKAADKYSKALFHLNEYVQEPFKQIAVEFPPSIDPLSDKNKQLPKKVIEEIALKYNIDLNRPIVTVVARFDPWKDLKSAIDVYRILKEKVDIQLLIVSAMAKDDPEGWILFEDILRYAGTDPDIHFLTDLKGVGHIEVNAFQTISTVGLHTATKEGFGLVISEMLWKQNPVVARPVGGVKVQVVDGFNGFLRNDIKELADAIYELITNESLRKQLGRNARKVVKEKFLTTSHLKRYLKVIKEVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGU9</accession>
    <name>B7IGU9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_853</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75313">
        <property type="protein sequence ID" value="ACJ75313"/>
        <property type="gene ID" value="THA_853"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_853"/>
    <dbReference type="HOGENOM" id="CLU_2902811_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="62" mass="6940" checksum="EA8200BB374D32CB" modified="2009-02-10" version="1">MLSIIEKISSSKIPTISLVLSFIPKIIFPPEPLANATTVFKKDSIFLAESLSLNSKFYLHLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGY2</accession>
    <name>B7IGY2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase, group 1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_886</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579861.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_886"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75346">
        <property type="protein sequence ID" value="ACJ75346"/>
        <property type="gene ID" value="THA_886"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_886"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_0_3_0"/>
    <dbReference type="OMA" id="PHAWSFE"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="16"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="190"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="375" mass="43335" checksum="9C94069D57456202" modified="2009-02-10" version="1">MRKKILHIVTRSDWTGVQKVLYNIVSGLKSNYSDQFEVEVAAGKENGMLFKELEKIGIRYYVLENLVREINPIKDLKAYFELKKLIKNGNYDIVHIHSSKAGVLGRIAAKRCGVKKVIYTYHGFWGIEQYKGFKKKFLILAERIAAKYSDYLVFLCNREKQKAEKYKIGKPHQYVIIPNAILPIENVQKGKLRKELSLPDNVKIIGNVARLDPPKNPIRFLEIAKKIINVRSDVVFVWIGESIVNDFYGKIVQNYLSKYPILRERVYFLPFRNDAPDLMTDFDLFLLTSDSEGAPLVILEARGNGIPIVSTDVGCVSEMIEEDFVSNDDYCLSKIVLDLLDKNINLESNKENYYKEFLVKYVSIYNEFMLDRDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH37</accession>
    <name>B7IH37_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase, 2-nitropropane dioxygenase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_942</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100850.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_942"/>
    <dbReference type="EnsemblBacteria" id="ACJ75401">
        <property type="protein sequence ID" value="ACJ75401"/>
        <property type="gene ID" value="THA_942"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_942"/>
    <dbReference type="eggNOG" id="COG2070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038732_0_1_0"/>
    <dbReference type="OMA" id="ATYECDA"/>
    <dbReference type="OrthoDB" id="275153at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMO" evidence="1">
        <location>
            <begin position="13"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="360" mass="39435" checksum="E91E7AA4F49DF074" modified="2009-02-10" version="1">MKLPVLKIGNLISKLPIIQGGMSVGISLSSLASAVANEGGIGVIGAAGIGMFEDDFDTNYRQANIRALRKEIRKARKLSDGIIGVNIMVALSDFSELIKASIEEHIDIIFMGAGLALDIPAKEIKESGVKIVPIVSSKRAASLIIKYWKKHYGILPDAIVVEGPKAGGHLGFKKEQIFDPDYSLEKILKEVVKEINVYEKKYGKKIPVIAAGGIYSGGDIYKFLKLGADGVQMATRFVATKECDASEEFKKMYVNCKKEDITIIDSPVGLPGRAIKNKFIEDVEKGGKKPIKCSWKCLKTCDFKKVPYCIAKALTNAKLGFLKEGFAFAGENAYKVKEIITVKELINRIKKEFSDAQFAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH58</accession>
    <name>B7IH58_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asp23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100923.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_963"/>
    <dbReference type="EnsemblBacteria" id="ACJ75422">
        <property type="protein sequence ID" value="ACJ75422"/>
        <property type="gene ID" value="THA_963"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_963"/>
    <dbReference type="eggNOG" id="COG1302">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167646_0_0_0"/>
    <dbReference type="OMA" id="NIQRMTE"/>
    <dbReference type="OrthoDB" id="1873879at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005531">
        <property type="entry name" value="Asp23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03780">
        <property type="entry name" value="Asp23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="111" mass="12568" checksum="50C668D285ACC318" modified="2009-02-10" version="1">MDFEQGSINISDEVLKEIAFRSFLEVLELSADDKDAKKLKKNIEIERTPDDNVIVNVKTVAPYGKSLVEFGKKIMQNISENIQRMTELQVSAVNVSIIDVVEKIEPAEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH78</accession>
    <name>B7IH78_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Permease of the drug/metabolite transporter, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_983</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100959.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_983"/>
    <dbReference type="EnsemblBacteria" id="ACJ75442">
        <property type="protein sequence ID" value="ACJ75442"/>
        <property type="gene ID" value="THA_983"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_983"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_986715_0_0_0"/>
    <dbReference type="OMA" id="WTITFTI"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="137"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="282" mass="31654" checksum="2051C2B2B3ECFA9C" modified="2009-02-10" version="1">MIYAILSAVLMGSTAIFNKFALKDFDPMFATVINSLLAGIFSLFFLKKFQKINMYVIFVGLFNAVGLLFMFMGLSKLDPGIAMVLGTSYYIFTSLYSVLLFKEKISMSVFFQIAVNIFGIILIIMPSYKVENLNLVGGIFSICSALFFATSNSVAKLSKIQADFLVFANNIITFLVISILYFSIYGTSSLSFSLRGFIFLTIASFIGSFLGLYFFYKSLSKIGFSIANIVRTLNPITSLLLSRLFFPNVITMRQWIGIIIVIFSIYRLNVLINRTKVELKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH91</accession>
    <name>B7IH91_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100991.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_996"/>
    <dbReference type="EnsemblBacteria" id="ACJ75455">
        <property type="protein sequence ID" value="ACJ75455"/>
        <property type="gene ID" value="THA_996"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_996"/>
    <dbReference type="eggNOG" id="ENOG502Z95P">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107962_0_0_0"/>
    <dbReference type="OMA" id="MAFHRIT"/>
    <dbReference type="OrthoDB" id="1441319at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="213" mass="24374" checksum="1A3B65F621DDB887" modified="2009-02-10" version="1">MKKGDFIWLGLFSLIVFLLVFKPTHQVYIALNRAHPYFMGFLKVSILATMGELLSIRLQKGKYLKPYGLFYRFLVWGFLGMCFVLVFELFASGTDVVMKKGLLPGNNKILHAFFTSALMNLIFAPTFMAFHRITDTYIDLGKGKIANIFSIKFSSVVDYIDWQKFFGFVILKTIPFFWIPAHTITFLLPPEYRVLTAAMLSIVLGVLLSIRKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHA2</accession>
    <name>MUTL_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA mismatch repair protein MutL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mutL</name>
        <name type="ordered locus">THA_1008</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579927.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHA2"/>
    <dbReference type="SMR" id="B7IHA2"/>
    <dbReference type="STRING" id="484019.THA_1008"/>
    <dbReference type="EnsemblBacteria" id="ACJ75466">
        <property type="protein sequence ID" value="ACJ75466"/>
        <property type="gene ID" value="THA_1008"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1008"/>
    <dbReference type="eggNOG" id="COG0323">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004131_4_3_0"/>
    <dbReference type="OMA" id="AHERIMY"/>
    <dbReference type="OrthoDB" id="764332at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032300">
        <property type="term" value="C:mismatch repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00149">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020667">
        <property type="entry name" value="DNA_mismatch_repair_MutL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="DNA_mismatch_repair_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="PTHR10073"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="SSF118116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000192187" description="DNA mismatch repair protein MutL">
        <location>
            <begin position="1"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00149"/>
        </source>
    </evidence>
    <sequence length="568" mass="65206" checksum="A9D79289089E3FD5" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHG3</accession>
    <name>B7IHG3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Precorrin-2 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1071</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1071"/>
    <dbReference type="EnsemblBacteria" id="ACJ75527">
        <property type="protein sequence ID" value="ACJ75527"/>
        <property type="gene ID" value="THA_1071"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1071"/>
    <dbReference type="eggNOG" id="COG2243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076014_2_1_0"/>
    <dbReference type="OMA" id="MEYPVTT"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030788">
        <property type="term" value="F:precorrin-2 C20-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11645">
        <property type="entry name" value="Precorrin_2_C20_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012382">
        <property type="entry name" value="CobI/CbiL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036427">
        <property type="entry name" value="Precrrn-2_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="198" mass="22265" checksum="98518ABEBDA67E68" modified="2009-02-10" version="1">MAAIEALKSSKYIFCPRTESGSIAAELVKKYVQKDIEFIDFPMGKDIKIDVSRILKSLLENKNVSFVSIGDPLFYSTFLQIMDAVKSYGIEVRVFPTITSFSFAASKINLPIALKDEGISVVPGTNLKLLEKAVNIFDNIVVIKPNRNFLKIVDILKDKGFKIYIFENLGFENERVFVEDIPESIGYLSIIIAKRGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHG8</accession>
    <name>B7IHG8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8 13">Glutamyl-tRNA reductase</fullName>
            <shortName evidence="8">GluTR</shortName>
            <ecNumber evidence="4 8 13">1.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 17" type="primary">hemA</name>
        <name evidence="17" type="ordered locus">THA_1076</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 13">
            <text>(S)-4-amino-5-oxopentanoate + NADP(+) + tRNA(Glu) = H(+) + L-glutamyl-tRNA(Glu) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:12344"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="EC" id="1.2.1.70"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8 13">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 13">Belongs to the glutamyl-tRNA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.70" evidence="4 8 13"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579982.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1076"/>
    <dbReference type="EnsemblBacteria" id="ACJ75532">
        <property type="protein sequence ID" value="ACJ75532"/>
        <property type="gene ID" value="THA_1076"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1076"/>
    <dbReference type="eggNOG" id="COG0373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035113_1_0_0"/>
    <dbReference type="OMA" id="CELKAME"/>
    <dbReference type="OrthoDB" id="1358620at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00316"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008883">
        <property type="term" value="F:glutamyl-tRNA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00087">
        <property type="entry name" value="Glu_tRNA_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000343">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015896">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015895">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018214">
        <property type="entry name" value="GluRdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036453">
        <property type="entry name" value="GluRdtase_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036343">
        <property type="entry name" value="GluRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00745">
        <property type="entry name" value="GlutR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05201">
        <property type="entry name" value="GlutR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000445">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69075">
        <property type="entry name" value="SSF69075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69742">
        <property type="entry name" value="SSF69742"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01035">
        <property type="entry name" value="hemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00747">
        <property type="entry name" value="GLUTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 11" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 8 13" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GlutR_N" evidence="16">
        <location>
            <begin position="12"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Shikimate_DH" evidence="15">
        <location>
            <begin position="153"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="GlutR_dimer" evidence="14">
        <location>
            <begin position="294"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="45"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="98"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 9">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <begin position="169"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for activity" evidence="8 12">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ACJ75532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="372" mass="43704" checksum="D461B5D85AD5AC15" modified="2009-02-10" version="1">MLNNLKMIGIGRNAPLYILEKFDFDEKEIIELLKSKVDEIAVLKTCHRREIYFIGDLKKLPFDLDENVEGYLIQRTELDVVRHLFKVSSGLDSMVLGEHQILSQVKNTYKNFCEGKLLKRIFNEAISVGKEVREKTGVSKFPLSISYIAVKLIEELKGKISEKDIFVIGTGQMGQKVVKYLVDRNANVYISNRTKSKALEIKNKYPGVHLVDFSKKYEFISKSDVVISATNAPHYVVEKKKVNTRKKKLFIDLSMPRNIDPKLSKLHDLYTLDDLQKISEKYRLLRKEKIDMMQKILEQRIEKFLNWYYSRNLKEKIEDVIKMRDIVVEEEFKRLVNKVEDNKNLEKIKESLRRCANRMASYHIQYLKGERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHH4</accession>
    <name>B7IHH4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cobalt transport protein CbiM</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Energy-coupling factor transporter probable substrate-capture protein CbiM</fullName>
            <shortName evidence="11">ECF transporter S component CbiM</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">cbiM</name>
        <name evidence="12" type="ordered locus">THA_1082</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of the energy-coupling factor (ECF) transporter complex CbiMNOQ involved in cobalt import.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms an energy-coupling factor (ECF) transporter complex composed of an ATP-binding protein (A component, CbiO), a transmembrane protein (T component, CbiQ) and 2 possible substrate-capture proteins (S components, CbiM and CbiN) of unknown stoichimetry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell inner membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the CbiM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579988.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1082"/>
    <dbReference type="EnsemblBacteria" id="ACJ75538">
        <property type="protein sequence ID" value="ACJ75538"/>
        <property type="gene ID" value="THA_1082"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1082"/>
    <dbReference type="eggNOG" id="COG0310">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052508_3_0_0"/>
    <dbReference type="OMA" id="ANVFSMG"/>
    <dbReference type="OrthoDB" id="1632785at2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01462">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018024">
        <property type="entry name" value="CbiM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002751">
        <property type="entry name" value="CbiM/NikMN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43627">
        <property type="entry name" value="PTHR43627"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01891">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00123">
        <property type="entry name" value="cbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 11" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0170">Cobalt</keyword>
    <keyword evidence="2 11" id="KW-0171">Cobalt transport</keyword>
    <keyword evidence="8 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ75538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="235" mass="25712" checksum="4756D9D89E2FBD6C" modified="2009-02-10" version="1">MRFLMALLLVFVVPSFGFSMHIMEGFLPPSHAAFWYILSIPFFAYGLFAIRRILKENPKQKMLLAFVAAFAFVLSAMKIPSVTGSCSHPTGVGLGAIIFGPTTMTVLGAIVLLFQALLLAHGGITTLGANVFSMAIVGPLVSYFVYKLLMKTGKRTIAVFLAAFLGDLFTYVTTSFQLALAFPDKSYGIWYSFAKFLGIFAVTQIPLAIIEGFLTVVVFDMILKYNEEIALGGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHJ2</accession>
    <name>B7IHJ2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1100"/>
    <dbReference type="EnsemblBacteria" id="ACJ75556">
        <property type="protein sequence ID" value="ACJ75556"/>
        <property type="gene ID" value="THA_1100"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1100"/>
    <dbReference type="HOGENOM" id="CLU_3318379_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="39" mass="4624" checksum="7EF2BB1128C5B7A1" modified="2009-02-10" version="1">MQKIIFKNQTSMLNKSFLNVNMMIMCGMWKNWPPLSFAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHJ3</accession>
    <name>B7IHJ3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbamate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">arcC</name>
        <name evidence="4" type="ordered locus">THA_1104</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the carbamate kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101201.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1104"/>
    <dbReference type="EnsemblBacteria" id="ACJ75557">
        <property type="protein sequence ID" value="ACJ75557"/>
        <property type="gene ID" value="THA_1104"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1104"/>
    <dbReference type="eggNOG" id="COG0549">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076278_0_0_0"/>
    <dbReference type="OMA" id="QPMDVAG"/>
    <dbReference type="OrthoDB" id="901370at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008804">
        <property type="term" value="F:carbamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006525">
        <property type="term" value="P:arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04235">
        <property type="entry name" value="AAK_CK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003964">
        <property type="entry name" value="Carb_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30409">
        <property type="entry name" value="PTHR30409"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000723">
        <property type="entry name" value="Carbamate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00746">
        <property type="entry name" value="arcC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="313" mass="34431" checksum="7CF78053C38CCAD9" modified="2009-02-10" version="1">MKKLAVVAIGGNAINRPGEKPTAENMFKNIKVTASYLADMIELGYRIIITHGNGPQVGNLLVQQDIAKDTIPPFPMDVLGAMTQGYLGYMISQELKNVLNERKIDKEIATVVTQIVVNKDDPGFKNPTKPVGPFYSEEEAKKIMEEKGWIFKEDAGRGWRRVVPSPIPLDVIEKRTIKDLVENDVIVVAGGGGGIPVIIDEKGEIKGVEAVIDKDRASALIAKELDADEFIILTAVEKVYLNFNKPDQKPLDTITVKEAEKYMNEGHFAKGSMLPKIEACISFVTSTNRPALITDMEKLKDALEGKTGTRIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHK4</accession>
    <name>B7IHK4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tetratricopeptide repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1115"/>
    <dbReference type="EnsemblBacteria" id="ACJ75568">
        <property type="protein sequence ID" value="ACJ75568"/>
        <property type="gene ID" value="THA_1115"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1115"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1229396_0_0_0"/>
    <dbReference type="OMA" id="KIQYMIG"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="165"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="225" mass="26499" checksum="D2E4BB0C1BEE22CC" modified="2009-02-10" version="1">MILISVFSFSYDIVDLQVAVGERNQEKVLSILSSLEYEKLPLEFKCEVILAYTDLYFWGENIPKNDYQELAKKYVDELLEKEQTYWKVYYAAALVYGHYVQKNYLLALFYSSKVFNYAKKSVELGQDQYLAHLLYGILNLETPFGKLELAEKHLLKALELNPNHVYTYVELGKLYEKKKECKKAIEMYEKALKIPGMKIWSYINKEGKMEARQRLSEVEKRCTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHL0</accession>
    <name>B7IHL0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1121</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580013.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1121"/>
    <dbReference type="EnsemblBacteria" id="ACJ75574">
        <property type="protein sequence ID" value="ACJ75574"/>
        <property type="gene ID" value="THA_1121"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1121"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_0"/>
    <dbReference type="OMA" id="GETVFFY"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="247" mass="27947" checksum="808B9CD9E730A7B8" modified="2009-02-10" version="1">MIKARNLTKKFGNFVAVDNINLTVEKGKIFGFLGSNGAGKTTTIRMLTGSLRPTSGEIEILGLNMKTHELEIKRRIGVVPDEPRIYDHLKGYEYLEFVMDIYRVNGKEVKDRIQELSEAFGVDYLFKQVSDMSHGMKQKLMLISVLMRKPEVIFLDEPTVGLDAKSAKILKELLKKYASQGTTIFLTTHILEIAEKMCDEIAIIDKGKIIAQGTMETLRNGENKSLEDLFLELTAQEEDIKNIVEML</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHM2</accession>
    <name>B7IHM2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1133</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580023.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1133"/>
    <dbReference type="EnsemblBacteria" id="ACJ75586">
        <property type="protein sequence ID" value="ACJ75586"/>
        <property type="gene ID" value="THA_1133"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1133"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_0"/>
    <dbReference type="OMA" id="FAFMPQR"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="218" mass="24754" checksum="02D9BE8BFCDE9731" modified="2009-02-10" version="1">MLLKLENVSKNFGKLCIFKNISLSISKGEKVALLGPSGCGKTTLLRIIAGLEDADGKIEKNFDKIGYIFQESRLIPWKTIYENLKFVSEDSKKIDNILKSLKLYEFKDYYPIKLSGGMKQRVNLARALLVEPDLLLLDEPFSSLDIHIKWNLIKDLNTQIDKNHITTILVTHDLKEALMLSDKIYVLSNRPAKIIGEFLTSQINESDLSNEIIKLWGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHR2</accession>
    <name>B7IHR2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="7">Glu-ADT subunit A</shortName>
            <ecNumber evidence="7">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">gatA</name>
        <name evidence="9" type="ordered locus">THA_1175</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101373.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1175"/>
    <dbReference type="EnsemblBacteria" id="ACJ75626">
        <property type="protein sequence ID" value="ACJ75626"/>
        <property type="gene ID" value="THA_1175"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1175"/>
    <dbReference type="eggNOG" id="COG0154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_0"/>
    <dbReference type="OMA" id="EVSCPHF"/>
    <dbReference type="OrthoDB" id="1239251at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="48"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="456" mass="50388" checksum="297B4008C28226B4" modified="2009-02-10" version="1">MKNFKDMTISDVLKENRSFVGESISRIKKIDEKINAFITVVENANGKIPIAIKDNIVTKGIRTTCASKILENFVPPYNATVVEKLIANDFAIVGKTNLDEFAMGTGTEYSAFFVTKNPWDHERVAGGSSGGSAAAVASGQVVAALGSDTGGSIRQPAAFCGVVGFKPTYGLVSRYGLVAFASSLDQIGPITKTTKDAAYLMNVIYGKDEKDATTVDRKINFEEYLESDFSNVKVAYPEEVFAEGVEEGVKERFEEFVKFLQRKGIKVDKVSFKELSYSVATYYIIAPSEVSSNLSRFDGIRYGKRDEQEGLLQTYLNSRKNLGKEVIRRIMIGTFTLSAAYYDAYFEKAQKVRRIISNKLNDLLKEYDFIITPTSPITAFKIGEVKDPLVYYMMDIFTIPANLAGIPAISIPFGFSNGLPVGMQIMGKRFDDAKVLGFANYIEKDIPVVLPGEEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHU8</accession>
    <name>B7IHU8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 9">50S ribosomal protein L23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">rplW</name>
        <name evidence="10" type="ordered locus">THA_1217</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 50S ribosomal subunit. Contacts protein L29, and trigger factor when it is bound to the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101443.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1217"/>
    <dbReference type="EnsemblBacteria" id="ACJ75662">
        <property type="protein sequence ID" value="ACJ75662"/>
        <property type="gene ID" value="THA_1217"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1217"/>
    <dbReference type="eggNOG" id="COG0089">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037562_3_2_0"/>
    <dbReference type="OMA" id="FEVDHRA"/>
    <dbReference type="OrthoDB" id="1978865at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_B">
        <property type="entry name" value="Ribosomal_L23_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_L23/L25_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_L25/23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="PTHR11620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="101" mass="11814" checksum="7DD2E932E6DDAD99" modified="2009-02-10" version="1">MKNYADVIIRPIVSEKSYYAREDRKYVFEVSKDANKPLIKEAVEKLFNVKVEKVNVINVKPKPKRDLRRGAMARQGYTKSWKKAIVTLKEGYTIKELEGEH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHV4</accession>
    <name>RL29_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmC</name>
        <name type="ordered locus">THA_1223</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101450.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHV4"/>
    <dbReference type="SMR" id="B7IHV4"/>
    <dbReference type="STRING" id="484019.THA_1223"/>
    <dbReference type="EnsemblBacteria" id="ACJ75668">
        <property type="protein sequence ID" value="ACJ75668"/>
        <property type="gene ID" value="THA_1223"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1223"/>
    <dbReference type="eggNOG" id="COG0255">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158491_5_2_0"/>
    <dbReference type="OMA" id="RFQMATS"/>
    <dbReference type="OrthoDB" id="2063644at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_L29/L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_L29/L35_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="SSF46561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000121829" description="50S ribosomal protein L29">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <sequence length="66" mass="7888" checksum="55C648CBF65F514B" modified="2009-02-10" version="1">MKAAELRNLTNEELMNLLEEKKRTLMNLRFQNVLGQLTDYSQISKTRKDIARIKTILRERELGVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHY4</accession>
    <name>B7IHY4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endonuclease Cas1</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cas1</name>
        <name evidence="10" type="ordered locus">THA_1253</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas2 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580091.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1253"/>
    <dbReference type="EnsemblBacteria" id="ACJ75698">
        <property type="protein sequence ID" value="ACJ75698"/>
        <property type="gene ID" value="THA_1253"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1253"/>
    <dbReference type="eggNOG" id="COG1518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052779_2_0_0"/>
    <dbReference type="OMA" id="AYKLIKH"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09722">
        <property type="entry name" value="Cas1_I-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019858">
        <property type="entry name" value="CRISPR-assoc_Cas1_HMARI/TNEAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43219">
        <property type="entry name" value="PTHR43219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03641">
        <property type="entry name" value="cas1_HMARI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="330" mass="39154" checksum="DD20AC0CC5B8EBAD" modified="2009-02-10" version="1">MKKDIYIFNSGELKRKDNTICFESSEGKKYLPIENINNLWIFGEVELNKRFLDFASENGILIHFFNFYGYYTGTFYPREHLNSGFVILKQAEHYLDNEKRIKLARKFVEGAVENLLVVLKYYKNRGYELEDEIDDIKNKKEGIYSSQDVETLMSFEGNIRDIYYKCFDKITKKEEFAFEKRSRRPPLNKMNSLISFGNSLLYTTVLGEIYQTQLDPRIGYLHSTNNRRFTLNLDVAEIFKPSIVDRVIFSLVNKNVLSSKDFDKQLNGIVLNNSGKKKFIEEYNKKLETVLKYTKLNAFVSYKRLIRLELYKVQKHITEDEEYQPFVARW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHY5</accession>
    <name>B7IHY5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CRISPR-associated exonuclease Cas4</fullName>
            <ecNumber evidence="6">3.1.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1254</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Mg(2+) or Mn(2+) required for ssDNA cleavage activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CRISPR-associated exonuclease Cas4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.12.1" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1254"/>
    <dbReference type="EnsemblBacteria" id="ACJ75699">
        <property type="protein sequence ID" value="ACJ75699"/>
        <property type="gene ID" value="THA_1254"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1254"/>
    <dbReference type="eggNOG" id="COG1468">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133784_0_0_0"/>
    <dbReference type="OMA" id="ICKTKLW"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013343">
        <property type="entry name" value="CRISPR-assoc_prot_Cas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00372">
        <property type="entry name" value="cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 6" id="KW-0464">Manganese</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas_Cas4" evidence="7">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="143" mass="16894" checksum="C09F5D969E16E507" modified="2009-02-10" version="1">MTHEIVPDQYNENIDLGRFIHEFYYKRDKKEIRFGNVVFDILYEKNNRLIIGETKKSSKFSEASKWQLIYYLKILKDAGIIAEGMLSFPEERKKIEVKLSAEAELELEKILKDIDDIINQPNPPKVSKNNFCKNCGYKEYCFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHY8</accession>
    <name>B7IHY8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Crispr-associated helicase Cas3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">THA_1257</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the CRISPR-associated nuclease Cas3-HD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the central section; belongs to the CRISPR-associated helicase Cas3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580095.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1257"/>
    <dbReference type="EnsemblBacteria" id="ACJ75702">
        <property type="protein sequence ID" value="ACJ75702"/>
        <property type="gene ID" value="THA_1257"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1257"/>
    <dbReference type="eggNOG" id="COG1203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2254">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010123_1_1_0"/>
    <dbReference type="OMA" id="EGWNSEI"/>
    <dbReference type="OrthoDB" id="1149839at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035011">
        <property type="entry name" value="Cas3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006483">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038257">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006474">
        <property type="entry name" value="Helicase_Cas3_CRISPR-ass_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF5">
        <property type="entry name" value="PTHR47959:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01587">
        <property type="entry name" value="cas3_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01596">
        <property type="entry name" value="cas3_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51643">
        <property type="entry name" value="HD_CAS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD Cas3-type" evidence="11">
        <location>
            <begin position="3"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="263"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="475"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ75702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="783" mass="92072" checksum="D6982A921391158B" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHZ7</accession>
    <name>B7IHZ7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1266</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580104.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1266"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75711">
        <property type="protein sequence ID" value="ACJ75711"/>
        <property type="gene ID" value="THA_1266"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1266"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_32_1_0"/>
    <dbReference type="OMA" id="VSSWVNQ"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022622">
        <property type="entry name" value="DUF3492"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11997">
        <property type="entry name" value="DUF3492"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DUF3492" evidence="2">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="278"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="468" mass="54242" checksum="12EFEAE53F8E2100" modified="2009-02-10" version="1">MKVGIIFEGTYPYIVGGVSSWGNSLISNMKDVEFCVIHVGDVPKKKAPKYNFPENVTDYFEYYLFANYKFPKKREIGKEITNALENIISYPFDENLMGKDLYELFKKNPSLDFTSIFKSQFYWDTIVSFYEKYLPYENFTNYYWTLYSMLIPSLNSMTVDLPKCDIYHTITTGYAGISAILNGLKYNVPVILTEHGIYHRERQLEILRADWIKEEFKTGWIKLFNTISSVVYKGSDKITTLFSENQKYEKEFCSKEEKFLIIPNGIDTNKFSNLTYRKSKTPFNVGLVGRVVEIKDIKTAIKAAKIVKQRIKDFKLFIIGPTDEEPEYFRECKELVDILNLQDTVEFTGMVDVKEYYPELNLMILSSVSEGQPLVILEAFAVGIPVVSTNVGACRELIYGSKEDFISPAGIVVKPKDFISLANAIIYMYENDKFRLNASETGKKRVHMRYRLDQMIENYKKLYLSVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICK5</accession>
    <name>B7ICK5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">OB-fold nucleic acid binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1287</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580120.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1287"/>
    <dbReference type="EnsemblBacteria" id="ACJ75732">
        <property type="protein sequence ID" value="ACJ75732"/>
        <property type="gene ID" value="THA_1287"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1287"/>
    <dbReference type="eggNOG" id="COG2374">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_514745_0_0_0"/>
    <dbReference type="OMA" id="GPFDVNI"/>
    <dbReference type="OrthoDB" id="382045at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="529" mass="59681" checksum="81187CF53EE5F2C8" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICK6</accession>
    <name>B7ICK6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carbohydrate kinase, fggy</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1288</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580121.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1288"/>
    <dbReference type="EnsemblBacteria" id="ACJ75733">
        <property type="protein sequence ID" value="ACJ75733"/>
        <property type="gene ID" value="THA_1288"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1288"/>
    <dbReference type="eggNOG" id="COG1070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_3_4_0"/>
    <dbReference type="OMA" id="AAYQKKW"/>
    <dbReference type="OrthoDB" id="1619686at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="Carb_kinase_FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="Carb_kinase_FGGY_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FGGY_N" evidence="1">
        <location>
            <begin position="4"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="FGGY_C" evidence="2">
        <location>
            <begin position="262"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="512" mass="58228" checksum="8A60586593D4A012" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICM8</accession>
    <name>B7ICM8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA primase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1310</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1310"/>
    <dbReference type="EnsemblBacteria" id="ACJ75755">
        <property type="protein sequence ID" value="ACJ75755"/>
        <property type="gene ID" value="THA_1310"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1310"/>
    <dbReference type="eggNOG" id="COG0358">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013501_3_3_0"/>
    <dbReference type="OMA" id="LQTRMEL"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03364">
        <property type="entry name" value="TOPRIM_DnaG_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013264">
        <property type="entry name" value="DNA_primase_core_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037068">
        <property type="entry name" value="DNA_primase_core_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034151">
        <property type="entry name" value="TOPRIM_DnaG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08275">
        <property type="entry name" value="Toprim_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Toprim" evidence="5">
        <location>
            <begin position="159"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="383"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="457" mass="53121" checksum="6D48105846C362F1" modified="2009-02-10" version="1">MEALKKLANEAGVKIEYSSSNSFQQLYFNFYKEIHKEYMEKLFDEKIALDYLFSRGFSEEEIKEYEFGFSPINSKIPQKIAQKLDIKSLKKFGFSTHDQFEGRLIIPIKNEYSNVIAFGGRLLGEGQPKYLNSYETDFFKKSKTLFLLDIAKDKIKTADFAIICEGYFDAIAFHRADFKNAVATLGTSFTKFHAFSIKKLTQNVILAFDTDSAGIKAALQSIKILISMQFNVMVAGKTKEKDPDEIYKTQGSSGLYSFLKEAIPAEEFIPYVLSKNYDLNNSNAIPLYTREIKNWEKIFENFPEKLETFRKTAEKISKTSITVSVPKPKQKTNLPTLDEMVIYLLLNNPQVDIEINPKIFNELTQEFIKSLKNLKNFSFEKLSKDLQKYIENILNKMEKFEINEDYINTVKKKIEEKTLEKRIEEIDKYLNSATEEEKRVLLQTRMELVRKLKKLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICP9</accession>
    <name>B7ICP9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fe-S oxidoreductase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1331</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1331"/>
    <dbReference type="EnsemblBacteria" id="ACJ75776">
        <property type="protein sequence ID" value="ACJ75776"/>
        <property type="gene ID" value="THA_1331"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1331"/>
    <dbReference type="eggNOG" id="COG1032">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042889_0_0_0"/>
    <dbReference type="OMA" id="AHYFMPL"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023980">
        <property type="entry name" value="CHP04013_B12-bd/rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04013">
        <property type="entry name" value="B12_SAM_MJ_1487"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="118"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="376" mass="42906" checksum="7CA73D2506DD0F2E" modified="2009-02-10" version="1">MSSIYSYRKDLELVDIRSFEEIFSYEKEGTVVLYSFMSFDLEAVVDELKKLKKSGYMVVAGGPHATAMPQQLLNLGFDYVFVGDGEENIRKFIDGKFPENRIYDGITKRVVLSDYPPFCPEKSLYMPIEITRGCPFSCGYCQTPRLAGKLVRHRPIEQIVEYSKIGVENNRKIARFISPNSFGYGSKNGVTPNPEAVDTLLYNLKKVGVEEIYFGTFPSDVRPESVNEEILKVIKKYVNNKYIVIGAQSGSERVLEMIKRGHNLEEIERALSLLKEYDFIPRVDFIFGFPFETDEDIEKTFEFIKKIVNKYKAKVHAHTFMPLPGTPLANIGPGVLTKKHYKFLGLLTSQGYLDGYWLKQEQLARRVADVNSSSPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICQ8</accession>
    <name>B7ICQ8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bacterial extracellular solute-binding protein, family 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1340"/>
    <dbReference type="EnsemblBacteria" id="ACJ75785">
        <property type="protein sequence ID" value="ACJ75785"/>
        <property type="gene ID" value="THA_1340"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1340"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_558664_0_0_0"/>
    <dbReference type="OMA" id="ISEIFNA"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBPb" evidence="2">
        <location>
            <begin position="9"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="2">
        <location>
            <begin position="224"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="436" mass="51829" checksum="BEC443A3D477149F" modified="2009-02-10" version="1">MYTYPQDNVKFFDENGKLIGISHKFIKILNERLKKYNINIEYKSTHSKNLSEILSALEKDEIQIFIGLAKNEERLEKFKFTNYPLWSVQLALLAKKENNDTENFHNKNIGVISNSKTEELSKIYFKDAKFVKFDGVSKALESLLKGKIDFLSYNSFLLGYYQSIYYDSLKLINIRNEKYRQYIAFSKNVDDKVVNIFDNVLKELFKSKEFEKLFDGYYGIIPGNIVELVTVDWPPYEYYENGKWQGIDYEILNLALNEMGLKLVTYKYPWSRCLQLMKSKSSDIVLTLIKTEDRKQYLYFTEIPISHGKDVYFFLKGNPNRNIAGYIKGYAYPKEFFKSPYKKVPVDSDERGMIFLSQKRIDLFITNLYVGLFYANKYNMDVEYSKTIKEQEYYVGFSKTYFGKFLSEQITKLLERFRKDGTYKKVYEKYNLQWEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICS2</accession>
    <name>B7ICS2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase_H_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580171.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1354"/>
    <dbReference type="EnsemblBacteria" id="ACJ75799">
        <property type="protein sequence ID" value="ACJ75799"/>
        <property type="gene ID" value="THA_1354"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1354"/>
    <dbReference type="eggNOG" id="COG2251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_690644_0_0_0"/>
    <dbReference type="OMA" id="LHYGETE"/>
    <dbReference type="OrthoDB" id="703621at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019993">
        <property type="entry name" value="RecB_nuclease_TM0106_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038720">
        <property type="entry name" value="YprB_RNase_H-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13482">
        <property type="entry name" value="RNase_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03491">
        <property type="entry name" value="TIGR03491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase_H_2" evidence="1">
        <location>
            <begin position="238"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="399" mass="47284" checksum="DB999D8822E40B5F" modified="2009-02-10" version="1">MQFCVSKIRTLLFCPKKFLNESKEEVFLDPYLEKKTKKVIKTGGKIKKGVLSTKLFNFKLIASNVEVISNNFEIYIISHRNGKKLYQYHYIEAAAYGYIFSKHTEKKINVIFKSKYYNVTMPWKKYLKDFIDIVEELKNYKEISPRINPECKFCKYNLECTNILIKEKNLSLLRGIGSAKLEKLFEHNIFTLDDLIKNKDVVENIFGEKGKRLILQATAFIENKPILISKVERLKNGIFLDIESYHDFHFLFGILKDNEYIPFFSFKREDEEKTFLQLIDFLKKQNAPIYHYFNYEPIQIKKLATKYKVKNKVKFEFIDIYKIISNSLAIPTISYSLKVLAKYFGFKWRTNLNGSSVIQKFEEYKKSKDKAILNEILMYNEDDVRATKLLFDIVNQFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICY1</accession>
    <name>B7ICY1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LAO/AO transport system ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1414</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101821.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1414"/>
    <dbReference type="EnsemblBacteria" id="ACJ75858">
        <property type="protein sequence ID" value="ACJ75858"/>
        <property type="gene ID" value="THA_1414"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1414"/>
    <dbReference type="eggNOG" id="COG1703">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043725_1_1_0"/>
    <dbReference type="OMA" id="DWRPPIV"/>
    <dbReference type="OrthoDB" id="1134430at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005129">
        <property type="entry name" value="GTPase_ArgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00750">
        <property type="entry name" value="lao"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="39"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="291" mass="31613" checksum="2001D8C6984E1809" modified="2009-02-10" version="1">MKELIEKLKQKDQKALAKLITYVENNEDTSFIEELPSSGSKIIGITGSPGAGKSSLVDAIITKLRETGKTVGIIAIDPSSPFTGGAFLGDRIRMKKHFLDDGVFIRSMGSGNSLGGLNESIFDVIKLMDAFGFDYILIETVGAGQSEIDIVFASDVVILVLSPGNGDEIQLLKAGIMEIGDIYVINKADLDGANSLKVQLEYTLSFSNVKKPIIETIATSGVGVEKLVESINDCFNNFSKNGELKKRLNRRIKKHAETIVLKKVKHIINSKETKSVSSLVEETIKSLCNMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID12</accession>
    <name>B7ID12_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glucokinase</fullName>
            <ecNumber evidence="2">2.7.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Glucose kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1446</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101879.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1446"/>
    <dbReference type="EnsemblBacteria" id="ACJ75889">
        <property type="protein sequence ID" value="ACJ75889"/>
        <property type="gene ID" value="THA_1446"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1446"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_0_4_0"/>
    <dbReference type="OMA" id="APNIDWR"/>
    <dbReference type="OrthoDB" id="1130699at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004654">
        <property type="entry name" value="ROK_glcA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00744">
        <property type="entry name" value="ROK_glcA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="310" mass="32841" checksum="77B1486878D64F9D" modified="2009-02-10" version="1">MNIVGVDLGGTYVKIGLVDSKNGKILKKSSIETKVELGGQEVVKRIANAILELTEGTDYHAVGIGSPGSIDKENGIVRFSPNFPDWHNFPLGSLLSELLGKNVYVENDANSFALGEKWFGAGIGKNHIVALTLGTGVGGGVISHGTLITGSTGIGAELGHVIINPNGPLCGCGNYGCLESYASATAIVRMATERKKKFPDSIIFKNEKVTAKAVFDAARDGDRLALMLRDEVVEALAIGITGFVHIFNPEVVIIGGGVSRAGDILFEPLRKRVNELVMPTFKDTFEIIQSPLVENAGILGAASIVLQREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID18</accession>
    <name>B7ID18_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CheA signal transduction histidine kinase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1452</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580231.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1452"/>
    <dbReference type="EnsemblBacteria" id="ACJ75895">
        <property type="protein sequence ID" value="ACJ75895"/>
        <property type="gene ID" value="THA_1452"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1452"/>
    <dbReference type="eggNOG" id="COG0643">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_464533_0_0_0"/>
    <dbReference type="OMA" id="VYPLPII"/>
    <dbReference type="OrthoDB" id="305000at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HPt" evidence="6">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="153"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="473"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="139"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="1">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="587" mass="67208" checksum="AA19D15A9BE3C196" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID32</accession>
    <name>B7ID32_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">YvyE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1466</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPACT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580242.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1466"/>
    <dbReference type="EnsemblBacteria" id="ACJ75909">
        <property type="protein sequence ID" value="ACJ75909"/>
        <property type="gene ID" value="THA_1466"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1466"/>
    <dbReference type="eggNOG" id="COG1739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083552_1_0_0"/>
    <dbReference type="OrthoDB" id="1777855at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020569">
        <property type="entry name" value="UPF0029_Impact_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="PTHR16301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00910">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0029" evidence="2">
        <location>
            <begin position="21"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="200" mass="22921" checksum="DDE99E1BF8CAB721" modified="2009-02-10" version="1">MNYIKYKTIFGVHEHKKNIERSIFIATLSHIESQEEAKEFIREISKKYNDATHNCPAYRVIENNQIIEFSSDNGEPSGTAGRPILGVLKKYDLLNVAVVVTRYFGGVKLGVRGLIDAYSSTVDELISNLKVTTLVPVPVYIIKVGYDEYGKAMQDLHYKGYEIINTEFFETFAHIKIRGNKKPEEYNVLNESITYIKEEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-01-19" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID34</accession>
    <name>PSUG_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pseudouridine-5'-phosphate glycosidase</fullName>
            <shortName evidence="1">PsiMP glycosidase</shortName>
            <ecNumber evidence="1">4.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">psuG</name>
        <name type="ordered locus">THA_1468</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible cleavage of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil. Functions biologically in the cleavage direction, as part of a pseudouridine degradation pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribose 5-phosphate + uracil = H2O + psi-UMP</text>
            <dbReference type="Rhea" id="RHEA:18337"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:58380"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="EC" id="4.2.1.70"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine-5'-phosphate glycosidase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.70" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580244.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ID34"/>
    <dbReference type="SMR" id="B7ID34"/>
    <dbReference type="STRING" id="484019.THA_1468"/>
    <dbReference type="EnsemblBacteria" id="ACJ75911">
        <property type="protein sequence ID" value="ACJ75911"/>
        <property type="gene ID" value="THA_1468"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1468"/>
    <dbReference type="eggNOG" id="COG2313">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012201_0_1_0"/>
    <dbReference type="OMA" id="RKLGHRD"/>
    <dbReference type="OrthoDB" id="1294333at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004730">
        <property type="term" value="F:pseudouridylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046113">
        <property type="term" value="P:nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1790.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01876">
        <property type="entry name" value="PsiMP_glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022830">
        <property type="entry name" value="Indigdn_synthA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007342">
        <property type="entry name" value="PsuG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909">
        <property type="entry name" value="PTHR42909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04227">
        <property type="entry name" value="Indigoidine_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110581">
        <property type="entry name" value="SSF110581"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000390553" description="Pseudouridine-5'-phosphate glycosidase">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="132"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01876"/>
        </source>
    </evidence>
    <sequence length="293" mass="32446" checksum="29E14476A5EF7999" modified="2009-02-10" version="1">MFISEKVQKALEENKPIVALESTVIAHGLPYPENLKVAQEFEDIVYENGCVPATIGILKGKVIVGLSKEQLIELVDDNPIKVGTREISYAIAMKKSAATTVSSTAKIASLAGIKVFATGGIGGVHRGEWDVSQDIIELSKTNIIVVSAGCKSILDIKKTLEFLETFQVLTVGYKTEYFPIFYNRLSKEKIYKVENADEIANIFNEKNKLKLESAILVANPIPEDYVLDNNEIEGYIKTIEKEIAEKDIHGKEVTPYMLKRLVELSNGKTLESNIVLLKNNVELACKIAQSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID55</accession>
    <name>B7ID55_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1491</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101979.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1491"/>
    <dbReference type="EnsemblBacteria" id="ACJ75932">
        <property type="protein sequence ID" value="ACJ75932"/>
        <property type="gene ID" value="THA_1491"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1491"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071528_0_0_0"/>
    <dbReference type="OMA" id="NIPFLLW"/>
    <dbReference type="OrthoDB" id="584516at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="329" mass="38254" checksum="E8B0EAB828BDB11C" modified="2009-02-10" version="1">MISFDFDFSMPEYTSGYILKSKNFRVSFIKFPTQYKFAEKGTEVVETYLFEPKKDIAGSLMILHGLGTSNIPFLLWMGTHLANAGVRTIVPILPGNFTRVADKSVSGKDYFSTDVERATRFWEHAVVDTLSVLNFAKNLNLWHENNCLLGFCLGGMLSVILNSLTDDFKKTILMTTGGDVGTLIWYSPTLAFMRREMKKGFGGDIIHKEEFLERFKKDIEKLKKFENVKQMQQSDIHPFLKIDPIAYAKFVKTERIIFIEALFDKALPKSTRKMLWEMLGKPRRHVIFSGHVTWLPFQYFLARFILKEMDANEFRRQARLMEKIKFEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID60</accession>
    <name>B7ID60_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580258.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1496"/>
    <dbReference type="EnsemblBacteria" id="ACJ75937">
        <property type="protein sequence ID" value="ACJ75937"/>
        <property type="gene ID" value="THA_1496"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1496"/>
    <dbReference type="eggNOG" id="COG0786">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034503_1_0_0"/>
    <dbReference type="OMA" id="LAWGQYV"/>
    <dbReference type="OrthoDB" id="1284248at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015501">
        <property type="term" value="F:glutamate:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015813">
        <property type="term" value="P:L-glutamate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004445">
        <property type="entry name" value="GltS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36178">
        <property type="entry name" value="PTHR36178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="417"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="444" mass="48776" checksum="B07C6C010B9CC4A5" modified="2009-02-10" version="1">MFTATVNTFFIISILYAIAILLKRFIPLLRKIIIPNSILAGFLGFLLGPNLLNIVKIDVDFLGKIIYHLMAIGFIALALRKVEKNGQKGYLGAGLIIVGTYLFQGILGVSLGFLINLFDKDFSPAIGYLIPLGFGQGPGQAYSIGMQWETLGLHSGSSIGLAIAAAGFGWATVGGLVILNLLLVKWKKSHQRTLIEKKAIEVRDYEFSDMDGMTIQMVIIGIVYFITFILLKIIEGVLSPLGNTGRTFSTVLWGFHFVIGAIVAMVFRKLYDKLKAKNIAKENYLNNFLLQRIAGVVFDFMVTASISAVVLAKIQKEFWGIFILTFAAGILTYFFVMMLTKKVFTKNEVENRIAFFGMLTGTISTGMALLREVDPALESGASENLVFGSGFSMLVGVPLIAILNFPAFAISNQKNILNLYGLIAMILYGLLLLVIGKLFLRSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID62</accession>
    <name>B7ID62_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">1-acyl-sn-glycerol-3-phosphate acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101999.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1498"/>
    <dbReference type="EnsemblBacteria" id="ACJ75939">
        <property type="protein sequence ID" value="ACJ75939"/>
        <property type="gene ID" value="THA_1498"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1498"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1694661_0_0_0"/>
    <dbReference type="OrthoDB" id="1485504at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PlsC" evidence="1">
        <location>
            <begin position="32"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="155" mass="17849" checksum="CEEB9F38E9C94F2E" modified="2009-02-10" version="1">MHPIWYLRIPLGYLIKKRYNLEILGSFPKPPFLLIANHTHNFDPLFISTFLKNPIYWIVAKGTFEKPILGPLFKSMNFIPKQKGEPDVSTIRKILKNLKENKIVGIFPEGSITWDGNFQEMPPGTDKLLNIVKVPIVAVKIMGGYLTKPRWANFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID73</accession>
    <name>B7ID73_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1509</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102031.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1509"/>
    <dbReference type="EnsemblBacteria" id="ACJ75950">
        <property type="protein sequence ID" value="ACJ75950"/>
        <property type="gene ID" value="THA_1509"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1509"/>
    <dbReference type="eggNOG" id="COG1988">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514687_0_0_0"/>
    <dbReference type="OMA" id="MNYEEHV"/>
    <dbReference type="OrthoDB" id="1891714at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="182" mass="20946" checksum="418C0B4BF22E1C6C" modified="2009-02-10" version="1">MPNFNTHVNFGIYFYPFVLLIYKIASQFLNFSMPEDKIIAIGFLIFVLSSDMPDIDHNHSLLHRFVKLLTISAAIYFEFTGEIIISYFNLNVEDVFLRFLFSFLFGILIGILYELIIPRHRGPLHTVWASLIYGGIIGYGTFYITSNLNNALFLGIISVSGYVLHLILDRYFVEKDGKLGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID90</accession>
    <name>B7ID90_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102056.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1526"/>
    <dbReference type="EnsemblBacteria" id="ACJ75967">
        <property type="protein sequence ID" value="ACJ75967"/>
        <property type="gene ID" value="THA_1526"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1526"/>
    <dbReference type="eggNOG" id="COG1956">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_882358_0_0_0"/>
    <dbReference type="OMA" id="ICGQAAE"/>
    <dbReference type="OrthoDB" id="1414332at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="1">
        <location>
            <begin position="179"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="312" mass="37414" checksum="EFDBED706D79FA7D" modified="2009-02-10" version="1">MRNIVENHVEEFLEILRYDKSHWGEFWSKTTNKYKFFKEIEEKLGEINFDSVERRELDKLLNDFREFAKENKDNVTTKIRKNAKELELNKEDFIVFLGVYPKDFDWIVVDFNGNYILFYNVYSLWKKGKLSKLSEAVYQAIIHFRNGEMNGIYYDKDELFDKLLDKLEKESKDDPVKYMRKICQYLYDEIPYYDWVGFYMINKDNVLELFEFVGEPTEHVKINIGEGICGQAAMLKDVFIVQDVSKETNYLSCSPKVKSEIVVPIFKNKEVIGELDIDSHYITPFDERDRKFLEKVCEDIPKIWDEKLLEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDA7</accession>
    <name>B7IDA7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Efflux cation efflux family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1545</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580270.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1545"/>
    <dbReference type="EnsemblBacteria" id="ACJ75984">
        <property type="protein sequence ID" value="ACJ75984"/>
        <property type="gene ID" value="THA_1545"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1545"/>
    <dbReference type="eggNOG" id="COG1230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_0_0_0"/>
    <dbReference type="OMA" id="WKRLIFS"/>
    <dbReference type="OrthoDB" id="948593at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="SSF160240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="296" mass="33072" checksum="5504093D9507DF9B" modified="2009-02-10" version="1">MHSHDHHGNSDIVGVKLLFSVFFNLAITLSEIIGGIISGSLALVSDALHNLSDTGALLTSYFARKISKKPRDLKFTYGYKRAETIAAIINTTILLAISFSLIVEGIRKVIQPTEINTDIMLIIAYIGLAGNLLTAILLFSHSKENLNLKSSFLHILSDLLSSIAIIITGHIMQFYNLYILDPIITFLISAYIIIESIHILKDAITITMQAVPDGVNFEIIKDKIESLPFVKDMHHTHIWSLDGNNLFIESHIKVTGKDYDNYLKEIKAILNKNGISHSTIQIESEYCDDFCIEEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDD0</accession>
    <name>B7IDD0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-sigma factor antagonist</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1569</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the anti-sigma-factor antagonist family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102296.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1569"/>
    <dbReference type="EnsemblBacteria" id="ACJ76007">
        <property type="protein sequence ID" value="ACJ76007"/>
        <property type="gene ID" value="THA_1569"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1569"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115403_6_4_0"/>
    <dbReference type="OMA" id="NNACKIT"/>
    <dbReference type="OrthoDB" id="1864829at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043856">
        <property type="term" value="F:anti-sigma factor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003658">
        <property type="entry name" value="Anti-sigma_ant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00377">
        <property type="entry name" value="ant_ant_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="3">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="112" mass="12689" checksum="3A2AF151A90792F9" modified="2009-02-10" version="1">MYEVTKLENIPVVKITGEIDISNAYKMKQFIHENILNNGEKYCILDLSHLKYIDSSGLGILVGLHKSFKLQGGEIVLVNMNENIKNLLRLTSLDRVLNIKETIKAAIDFLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDF3</accession>
    <name>B7IDF3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">General secretion pathway protein D, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1592</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the bacterial secretin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102342.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1592"/>
    <dbReference type="EnsemblBacteria" id="ACJ76030">
        <property type="protein sequence ID" value="ACJ76030"/>
        <property type="gene ID" value="THA_1592"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1592"/>
    <dbReference type="eggNOG" id="COG4796">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059922_0_0_0"/>
    <dbReference type="OMA" id="FVGTANP"/>
    <dbReference type="OrthoDB" id="870677at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001775">
        <property type="entry name" value="GspD/PilQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011662">
        <property type="entry name" value="Secretin/TonB_short_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004846">
        <property type="entry name" value="T2SS/T3SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00263">
        <property type="entry name" value="Secretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07660">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00811">
        <property type="entry name" value="BCTERIALGSPD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00965">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="STN" evidence="6">
        <location>
            <begin position="41"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="368" mass="41187" checksum="53FA069670314870" modified="2009-02-10" version="1">MKKYLVILIISLITFAFSVDIYFYQTDIRDALNQLGMQEGVSIIYDPLISGFISVEAYNITLEKALDLMLLPLGYYWTKIDNVYFVGTANPDSPNFALISKKYLLNLRFITFDDLYNALPKIMTNYIYKTPNKNQVLIYAPAKIASQIAETITLIDKQIPVAEIQVKIIEIDESDFKKFGVSWNNEENNTTSYNNGNLQLSLNPLNVNFNIIMDTLISSGNAKVLAEGTLKLKSNSTSQITGYTTVGFTIGKDNTYVEKKINTQIIISGHVFTDHANLTLKSIVESFIQNSSTFTPVGASVETNLDIKYEQPYYIAGLSFDSVTKQDGGIPGLKDIPIIGNLFKNSTYTSRKKNIVIFIKARLAGEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDI3</accession>
    <name>B7IDI3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="9">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 13" type="primary">alaS</name>
        <name evidence="13" type="ordered locus">THA_1622</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580302.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1622"/>
    <dbReference type="EnsemblBacteria" id="ACJ76060">
        <property type="protein sequence ID" value="ACJ76060"/>
        <property type="gene ID" value="THA_1622"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1622"/>
    <dbReference type="eggNOG" id="COG0013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_1_0_0"/>
    <dbReference type="OMA" id="YHHTMFE"/>
    <dbReference type="OrthoDB" id="91428at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="SSF101353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 9" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II_ALA" evidence="12">
        <location>
            <begin position="1"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="714"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="553"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="557"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="655"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="659"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ76060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="859" mass="97599" checksum="10DD523EE1DE477F" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDK8</accession>
    <name>PANB_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-methyl-2-oxobutanoate hydroxymethyltransferase</fullName>
            <ecNumber evidence="1">2.1.2.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ketopantoate hydroxymethyltransferase</fullName>
            <shortName evidence="1">KPHMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">panB</name>
        <name type="ordered locus">THA_1647</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = (6S)-5,6,7,8-tetrahydrofolate + 2-dehydropantoate</text>
            <dbReference type="Rhea" id="RHEA:11824"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodecamer; pentamer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PanB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.11" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580325.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDK8"/>
    <dbReference type="SMR" id="B7IDK8"/>
    <dbReference type="STRING" id="484019.THA_1647"/>
    <dbReference type="EnsemblBacteria" id="ACJ76085">
        <property type="protein sequence ID" value="ACJ76085"/>
        <property type="gene ID" value="THA_1647"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1647"/>
    <dbReference type="eggNOG" id="COG0413">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036645_1_0_0"/>
    <dbReference type="OMA" id="VLVWTDM"/>
    <dbReference type="OrthoDB" id="916845at2"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00003"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003864">
        <property type="term" value="F:3-methyl-2-oxobutanoate hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06557">
        <property type="entry name" value="KPHMT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00156">
        <property type="entry name" value="PanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003700">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881">
        <property type="entry name" value="PTHR20881"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02548">
        <property type="entry name" value="Pantoate_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000388">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00222">
        <property type="entry name" value="panB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000118131" description="3-methyl-2-oxobutanoate hydroxymethyltransferase">
        <location>
            <begin position="1"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="41"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00156"/>
        </source>
    </evidence>
    <sequence length="265" mass="28982" checksum="BAD6A997572F84C9" modified="2009-02-10" version="1">MTIQKILKMKNKEKIVMVTAYDAPTAKILEKAGVDIILVGDSLGNNVLGYDSTIPVTLEEMIIHLQAVRRGAPNSFIVADLPFLSYGYSIEESVKNAGLLIKNGANAVKIEGGAQNCEIIQKCLNIGIPVMGHIGFTPQSLNMFGGYKVQGKEESAKKKLLDNALALENCGVFSIVLEMVTESIAKEITEKLSIPTIGIGAGRYCDGQVLVFHDIVGLNPEFKPKFSKMYANTFEIMLNAIEKFKMEVKDKTFPSKENVFEEGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDL5</accession>
    <name>B7IDL5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Epoxyqueuosine reductase QueH</fullName>
            <ecNumber evidence="5">1.17.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Queuosine biosynthesis protein QueH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">THA_1654</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + epoxyqueuosine(34) in tRNA = A + H2O + queuosine(34) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:32159"/>
            <dbReference type="Rhea" id="RHEA-COMP:10345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10346"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:82831"/>
            <dbReference type="ChEBI" id="CHEBI:82834"/>
            <dbReference type="EC" id="1.17.99.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the QueH family.</text>
    </comment>
    <dbReference type="EC" id="1.17.99.6" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1654"/>
    <dbReference type="EnsemblBacteria" id="ACJ76092">
        <property type="protein sequence ID" value="ACJ76092"/>
        <property type="gene ID" value="THA_1654"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1654"/>
    <dbReference type="eggNOG" id="COG1636">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088177_1_1_0"/>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052693">
        <property type="term" value="F:epoxyqueuosine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003828">
        <property type="entry name" value="QueH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36701">
        <property type="entry name" value="PTHR36701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02677">
        <property type="entry name" value="QueH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ76092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="246" mass="29380" checksum="7B2C1628278A623B" modified="2009-02-10" version="1">MKEKIKYIYFYNPNIHPKSEYEKRLNEVLKLAKRWNLKIIESKYEPEVYFRYIKGLENLGINSPRCDKCIYLLLKNTAITAKINNFNSFATTLTSSPRKVLEKINKIGKIVEQEVNVKYIETYFRKGKEYQEALKFIKEQNIYRQAYCGCIFSKIELENKRKEKIEKSKKILKEYGITNIPVFPEKLVITKENFEIFSKNFIEIIKAIQPKILYVDYFVKDKYNLKEGWNKFGNYNQKIQILKGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDL8</accession>
    <name>B7IDL8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1657</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102464.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1657"/>
    <dbReference type="EnsemblBacteria" id="ACJ76095">
        <property type="protein sequence ID" value="ACJ76095"/>
        <property type="gene ID" value="THA_1657"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1657"/>
    <dbReference type="eggNOG" id="ENOG50338R8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_501452_0_0_0"/>
    <dbReference type="OMA" id="YEWGNEL"/>
    <dbReference type="OrthoDB" id="201994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="543" mass="61172" checksum="DA170BB1155193FE" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDL9</accession>
    <name>B7IDL9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Probable subtilase-type serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1658</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580331.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1658"/>
    <dbReference type="EnsemblBacteria" id="ACJ76096">
        <property type="protein sequence ID" value="ACJ76096"/>
        <property type="gene ID" value="THA_1658"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1658"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4978">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_15_6_0"/>
    <dbReference type="OMA" id="EPSYKRY"/>
    <dbReference type="OrthoDB" id="923655at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase_S8" evidence="6">
        <location>
            <begin position="172"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="476" mass="51861" checksum="DECB429432B2E563" modified="2009-02-10" version="1">MNKLFKYSFFGILVLFLILSSCGLNNITQNSNISNNTGSDIKLGSLDFEYEEGKVFVGYENREEVNKIVEALNGKIAVDLPQIKMVSIKFNGTVEEAYEKILKLGLKGIRYVEPSYKRYIIDPKPVDNNMINKNNETSLNSTNPRDDEEFSKYLWGLEALNVKDVWNMGYTGDGVIVAVVDSGVDGTHPDLKGQVIEGYRPKTGEILPAGTDSSFGGAHGTHVAGTIAASDNSLGIIGVAPDAKIMPIVIFDVEGYVGDDAVADGIIWAVDHGADILQNSWGGWGYSYTLKEAFDYALDRDVVVTVSAGNDHTDQHVHYPSGYPGIIQVAAVEFNGGKYRTTWFSNRSDAITVGAPGVDILSTVPQATALGYEGGELADSFGGLYAFYQGTSMACPHVSGVVALLKEKYPDAKVWQIRKLIEQGAVDIDKDGYDHDSGFGLVNAKNSINLSLPTEVEQHLMLKHSIVGKILYREYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDQ3</accession>
    <name>B7IDQ3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF4040 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1693</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580353.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1693"/>
    <dbReference type="EnsemblBacteria" id="ACJ76130">
        <property type="protein sequence ID" value="ACJ76130"/>
        <property type="gene ID" value="THA_1693"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1693"/>
    <dbReference type="eggNOG" id="COG1563">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173139_2_0_0"/>
    <dbReference type="OMA" id="ILAVEWR"/>
    <dbReference type="OrthoDB" id="2088292at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025383">
        <property type="entry name" value="MbhD-like_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13244">
        <property type="entry name" value="DUF4040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4040" evidence="8">
        <location>
            <begin position="10"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="79" mass="8353" checksum="FA77C742EC2D69C7" modified="2009-02-10" version="1">MTIVIIFVGILMIISAIYAIEAKKMLDSFISLSLLSLLSVFLFVIMKAPDVAITEAAVGAGLTTAVLILALQRVGGDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDV1</accession>
    <name>B7IDV1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1744</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580383.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1744"/>
    <dbReference type="EnsemblBacteria" id="ACJ76178">
        <property type="protein sequence ID" value="ACJ76178"/>
        <property type="gene ID" value="THA_1744"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1744"/>
    <dbReference type="eggNOG" id="ENOG5032WQ5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077686_0_0_0"/>
    <dbReference type="OMA" id="LEILFWY"/>
    <dbReference type="OrthoDB" id="881934at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="305" mass="36180" checksum="A11C401D258F6CC2" modified="2009-02-10" version="1">MIYDPEYLSRLTTLKGKRKVIVDILKSIKHMNSNNYKILINNLENKKLKEKLKKTNINYFIIYTSNLLHGNGSIISRLKMFKEINIEPNEIGEILFWANPKKFPFPDTSENYDKKYFEQKNKLLKKYKVSDFLELYVIESLNKETFLNDIISEINSITFHNLDRINWLREIIYELDPISKQKVREKISIHPYLKRALFSKPISPVILDGNNIAFWSIPPSTKNIYNLLETLSKAKDFYFPFYIVFDKNAKYMFKNDGIFKLPNVYFHSPADELIISLAKTKKGKIISKDKFRDWDKNIKKLILEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDW4</accession>
    <name>B7IDW4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1760"/>
    <dbReference type="EnsemblBacteria" id="ACJ76191">
        <property type="protein sequence ID" value="ACJ76191"/>
        <property type="gene ID" value="THA_1760"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1760"/>
    <dbReference type="HOGENOM" id="CLU_3085725_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="52" mass="6393" checksum="59BB30B28F64A95E" modified="2009-02-10" version="1">MLEDIEGSILLADRGYWQWEFIEKMKEKMKLYVRPSGRKGKEFMEREINRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDX9</accession>
    <name>B7IDX9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Cytidine deaminase</fullName>
            <ecNumber evidence="6 11">3.5.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Cytidine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">THA_1775</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 11">This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 11">
            <text>2'-deoxycytidine + H(+) + H2O = 2'-deoxyuridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13433"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15698"/>
            <dbReference type="ChEBI" id="CHEBI:16450"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>cytidine + H(+) + H2O = NH4(+) + uridine</text>
            <dbReference type="Rhea" id="RHEA:16069"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.5" evidence="6 11"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580401.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1775"/>
    <dbReference type="EnsemblBacteria" id="ACJ76206">
        <property type="protein sequence ID" value="ACJ76206"/>
        <property type="gene ID" value="THA_1775"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1775"/>
    <dbReference type="eggNOG" id="COG0295">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097262_0_1_0"/>
    <dbReference type="OMA" id="LTHFTCV"/>
    <dbReference type="OrthoDB" id="1895660at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047844">
        <property type="term" value="F:deoxycytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006262">
        <property type="entry name" value="Cyt_deam_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01354">
        <property type="entry name" value="cyt_deam_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ76206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="129" mass="13991" checksum="3EAD433AA88226A0" modified="2009-02-10" version="1">MNDKLIELAKKAQKNAYAPYSNFKVGAAILTKSGKIFTGANVENASYGLSCCAERNAIFKAVSEGETQFSKIVVVGDTEEPISPCGACRQVMAEFGDFEVILVGKNGNIKKTTVSELLPYNFDKENLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDZ0</accession>
    <name>B7IDZ0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="9">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 13" type="primary">argS</name>
        <name evidence="13" type="ordered locus">THA_1786</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580411.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1786"/>
    <dbReference type="EnsemblBacteria" id="ACJ76217">
        <property type="protein sequence ID" value="ACJ76217"/>
        <property type="gene ID" value="THA_1786"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1786"/>
    <dbReference type="eggNOG" id="COG0018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_0_1_0"/>
    <dbReference type="OMA" id="NKPLHLG"/>
    <dbReference type="OrthoDB" id="1146366at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="PTHR11956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="SSF55190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arg_tRNA_synt_N" evidence="12">
        <location>
            <begin position="2"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="DALR_1" evidence="11">
        <location>
            <begin position="424"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="9">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ76217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="543" mass="63741" checksum="8A01C1E1DA17E03A" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE06</accession>
    <name>B7IE06_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="15">ATP-PFK</shortName>
            <shortName evidence="15">Phosphofructokinase</shortName>
            <ecNumber evidence="15">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15 17" type="primary">pfkA</name>
        <name evidence="17" type="ordered locus">THA_1802</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 15">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 15">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade 'B1' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="15"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102739.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1802"/>
    <dbReference type="EnsemblBacteria" id="ACJ76233">
        <property type="protein sequence ID" value="ACJ76233"/>
        <property type="gene ID" value="THA_1802"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1802"/>
    <dbReference type="eggNOG" id="COG0205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020655_0_1_0"/>
    <dbReference type="OMA" id="KFAVICV"/>
    <dbReference type="OrthoDB" id="1421302at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00339">
        <property type="entry name" value="Phosphofructokinase_I_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012003">
        <property type="entry name" value="ATP_PFK_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012828">
        <property type="entry name" value="PFKA_ATP_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000532">
        <property type="entry name" value="ATP_PFK_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="SSF53784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02482">
        <property type="entry name" value="PFKA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="12 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 15" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11 15 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PFK" evidence="16">
        <location>
            <begin position="3"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="ADP binding; allosteric activator; shared with dimeric partner" evidence="15">
        <location>
            <begin position="21"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="15">
        <location>
            <begin position="126"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="15">
        <location>
            <begin position="170"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="ADP binding; allosteric activator" evidence="15">
        <location>
            <begin position="186"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="ADP binding; allosteric activator" evidence="15">
        <location>
            <begin position="214"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="15">
        <location>
            <begin position="250"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="72"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="102"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ACJ76233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="319" mass="34249" checksum="0C7ABDE36C0593C7" modified="2009-02-10" version="1">MKKIAVMTSGGDAPGMNAAIRAVVRYAVRNDIQVLGIQRGYAGLLDEDFIEMDFSSVGGIMEKGGTILRSSRCPEFVRKETRQEAAKILAKHGVEGLIVIGGEGSLHGAMFLEEEQKVPVVGIPGTIDNDIAMTDMSIGVDTCLNTVVDAIQKLKDTASSHERAFIVEVMGRSSGYIATVAGLVTGAEAIVIPEIPVNYEALGNKILKERERGKINCIVVVAEGAASAYTVARHLEHRIGYETRITILGHIQRGGSPTAFDRLLASRMGVAAVEFLKRGNSYVMTALQGGKIVPVKLDEVLKQKKSLNMELVELTALLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IED4</accession>
    <name>B7IED4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, LacI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1939</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580492.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1939"/>
    <dbReference type="EnsemblBacteria" id="ACJ76361">
        <property type="protein sequence ID" value="ACJ76361"/>
        <property type="gene ID" value="THA_1939"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1939"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_0_0"/>
    <dbReference type="OMA" id="EAGLWHR"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00036">
        <property type="entry name" value="HTHLACI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="333" mass="37892" checksum="7690689737B60F86" modified="2009-02-10" version="1">MAGIRDVAREANVSIATVSRVINGSDKVSEETRKKVLRAMRKLNFRPKPYFGNNEVFFKTIGVLVPDIRGYHYSDIVMAIESCAFENGFDVILSIPKADIDNEKHILEKYFKRKIDGIIVAELLGGENYIEKFLKSGIPLVVLDFSVEEINFDVVNVDNMMGAYNAIKYLYENGHRKILFIPGPEWSPAANEREKGVKRFLESVDDLEIYYTNIRGYNSQDGKDAIFEYLTKNSLNFTAIFAVNDWTAIGAIDALRIKGIKVPDDVSIIGFDDAPFLDYIEPKLTTVRQPRWELGYTAAQILIERITSKRSRLPRNVVIPPELVIRESVKKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEI9</accession>
    <name>B7IEI9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transporter, major facilitator family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1995</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580533.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1995"/>
    <dbReference type="EnsemblBacteria" id="ACJ76416">
        <property type="protein sequence ID" value="ACJ76416"/>
        <property type="gene ID" value="THA_1995"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1995"/>
    <dbReference type="eggNOG" id="COG0738">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055429_1_0_0"/>
    <dbReference type="OMA" id="WNYPKRN"/>
    <dbReference type="OrthoDB" id="1121832at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="354"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="6"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="387" mass="43518" checksum="965AC71D2011C1FD" modified="2009-02-10" version="1">MKEKLINLYGFLNFFTFGFVLVLIGPLIPVIEKGFSINHSLIGLSLSLGSVSFLIGSLVFGFLLEKFNTFNVVLTAGVIFSFGVFILFIMNSFTLFVFGNFMMNFGGAALEVSIPFLIGVSGTGKKAKKLNFLHSAFAIGAVISPIVSSFILRFTNLWRTTFAIGFITSLLPFIFLFLIKNTINEFHSEYVKEQISFKNLINFSLVILILALSLYVSYEMNFSSWISVFLHEVRSYSVSRSAIFPSFLWLGLFIGRLFFAKMPEKFGYKKWLLFVISFSLICSSLSVFLSINFFISSILIFFTGLFYATTYPTIQATIIEKYKSNKGIALSLASSITSLFSGGASYLIGLLGQYFGIFFGFLLILIFNILELMIIIIFKENQIFIEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEM9</accession>
    <name>B7IEM9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_35</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579309.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_35"/>
    <dbReference type="EnsemblBacteria" id="ACJ74543">
        <property type="protein sequence ID" value="ACJ74543"/>
        <property type="gene ID" value="THA_35"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_35"/>
    <dbReference type="eggNOG" id="ENOG50340ZH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1783971_0_0_0"/>
    <dbReference type="OMA" id="WAFVICI"/>
    <dbReference type="OrthoDB" id="1685794at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="146" mass="15983" checksum="3E2FA9D94845708E" modified="2009-02-10" version="1">MLGVTAYAVLVFIMNIVIGLVLLLGVIQQAKSSFGISSVVSWVFFAVSLAFFASGGFVNFYSKLGLSISPVAYLVIAIYSMLAASDISKLNIRKAMRKAILFSIIMIVFLIIDLIWVKDAAFVALFSIGWAYVITLIFGIKNRNIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEP7</accession>
    <name>B7IEP7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transketolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_53</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_53"/>
    <dbReference type="EnsemblBacteria" id="ACJ74561">
        <property type="protein sequence ID" value="ACJ74561"/>
        <property type="gene ID" value="THA_53"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_53"/>
    <dbReference type="eggNOG" id="COG0021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_3_1_0"/>
    <dbReference type="OMA" id="NSGHSGM"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016744">
        <property type="term" value="F:transketolase or transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="7">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="620" mass="69089" checksum="9D405DAFBAB98B59" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IER5</accession>
    <name>B7IER5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Serine protease MucD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_71</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1C family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579330.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_71"/>
    <dbReference type="EnsemblBacteria" id="ACJ74579">
        <property type="protein sequence ID" value="ACJ74579"/>
        <property type="gene ID" value="THA_71"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_71"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020120_1_1_0"/>
    <dbReference type="OMA" id="IIGINRQ"/>
    <dbReference type="OrthoDB" id="741829at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011782">
        <property type="entry name" value="Pept_S1C_Do"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02037">
        <property type="entry name" value="degP_htrA_DO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="260"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR611782-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="453" mass="49829" checksum="BA323401B1B3FBD2" modified="2009-02-10" version="1">MKKFSTILILAFFSLMIFSYVNPDYVSPVTKVVEEAAPAVVKVEATVYSTSYIDPFIEDFFKRWFGDIPKQYQQKGTSLGSGFIFDKEGYILTNFHVVDGAEEIKVSLLDGTEYKAEYIGGDKELDIAVLKIDPKGSDLPVLEFGDSDKIKIGEWAIAIGNPLGFQHTVTLGVVSAVGRKIPKPDNSGYYTNLIQTDAAINPGNSGGPLLDIHGQVIGINTAIIAPSEAMNIGFAIPINTAKRFIDSIIKTGKVEKAYLGVYMQTVTDDLKKALGLKVSKGVYIAQVVKNSPAEKAGLKEGDVILEVENMSVSSAGELASIIHNYTPGSKIKIKIDRKGKEIEIEVILGRAEESFEEEVEAQEFAGIIVKDIDNADREKYQIPEEVNGVIVVKSKNGFIKEGYVIYKMAISGKTYKIENINDWNNTIKNVKKGDYVAFFYYYKGATGIFSFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IER9</accession>
    <name>B7IER9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Bifunctional NAD(P)H-hydrate repair enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Nicotinamide nucleotide repair protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="11">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
                <ecNumber evidence="11">4.2.1.136</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="11">ADP-dependent NAD(P)HX dehydratase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="11">NAD(P)H-hydrate epimerase</fullName>
                <ecNumber evidence="11">5.1.99.6</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="10" type="primary">nnrE</name>
        <name evidence="9" type="synonym">nnrD</name>
        <name evidence="13" type="ordered locus">THA_75</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 11">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 10 11">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 9 11">
            <text>(6S)-NADHX + ADP = AMP + H(+) + NADH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 9 11">
            <text>(6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="10 11">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 11">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="11"/>
    <dbReference type="EC" id="5.1.99.6" evidence="11"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <source>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
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        </lineage>
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        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
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            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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        <property type="entry name" value="RNase_E/G"/>
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        <property type="entry name" value="S1_dom"/>
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        <property type="entry name" value="RNaseEG"/>
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        <property type="entry name" value="S1"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="37"/>
            <end position="115"/>
        </location>
    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="465" mass="54000" checksum="C5224DA0CAE830ED" modified="2009-02-10" version="1">MEKILVLNSIEDNLHYAIIENNKLVELFSDEEKKLTGNIYLGRVEKVVNALDAIFVNIGEKKNGFLRIKDIPEKYYDYFSVKEIKEGSKILIQVKKDPTGTKGPQLTANISIAGRYLVVFPFSNVNGVSKKIESEDERQRLYKIANEISNNNGFGVVIRTAAEGLNEKYIYSEAITLKEKLDDLLSNFKRKRKIQILYTEESLIDYILRERLTKEVTQVITNNIKHIEVIRKYLKIFPKRPKIEIIDGDSFEYTNVNKYFKELIKRTITLPSGGEIIIDKTEALTVIDVNSKHFTKGLNQQETAFKTNYEAVEEIFRQLRLRNIGGIVIIDFIDMNSEENKNKILEKVNEEIKKDKSKIEIFGFTKLGLLELSRKRTIKSFFENYVSTCPVCGGSGFIQSPRFLLKEIFAKIENAPKEAKEVIIKLHPYMKNYIDKSELKKRKDLNYHIHFTHTDPKTFEITWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEV0</accession>
    <name>B7IEV0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">M23 peptidase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103494.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_106"/>
    <dbReference type="EnsemblBacteria" id="ACJ74614">
        <property type="protein sequence ID" value="ACJ74614"/>
        <property type="gene ID" value="THA_106"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_106"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_860340_0_0_0"/>
    <dbReference type="OMA" id="ITATFME"/>
    <dbReference type="OrthoDB" id="1891666at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002854677" evidence="1">
        <location>
            <begin position="22"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M23" evidence="2">
        <location>
            <begin position="45"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="321" mass="37138" checksum="40D900920DEEFE31" modified="2009-02-10" version="1">MKKTVAFILLILITLSFSNFIPPVDDSYITSSFAEFRSTGNLPHFHGGIDFSTFSKEGIPIKAIYEGYVVRVELNDPIYGNVIVLQHPNGYRSLYAHLSNFNYTIENIIKSLQEEFQNQKIVINFPDNEIKFSQGDIIAYSGKTGEAVKPHCHLEIRNSDETLMFDPIDFLNVQAPNGEIILKELIINGKSYNYTEGETYSFKGEYPKIEINSYLFVNNNLLGLKEIKLYIANKLVYDILLDEVSKEEFYKPYIVYSKDSIAAGYIYKTYYKLYPEILGGPIKVNNFPTLNTNTDFFQVRIEAYDPWKRVKEFTFNLKRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEX0</accession>
    <name>B7IEX0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase, gnat family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_126"/>
    <dbReference type="EnsemblBacteria" id="ACJ74634">
        <property type="protein sequence ID" value="ACJ74634"/>
        <property type="gene ID" value="THA_126"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_126"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_3_2_0"/>
    <dbReference type="OMA" id="FQMEGCM"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="163" mass="19010" checksum="7E581EC96DA20C56" modified="2009-02-10" version="1">MYNGKLVRLRAYEKNDLEIARKFVNDPEVKKYLIPGIPFPLRVDDEEKWYNSLNPFGRGEYSFAIERIEDGKYIGGCGINKVDWKNSVAEVGIFLGKPYWNKGYGTDAMRVLVNFIFNEMNINKVKLEVFSFNERAIRSYEKVGFKIEGALRGRYLEMVNIIM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEY7</accession>
    <name>UVRB_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UvrABC system protein B</fullName>
            <shortName evidence="1">Protein UvrB</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Excinuclease ABC subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uvrB</name>
        <name type="ordered locus">THA_143</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The beta-hairpin motif is involved in DNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UvrB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579372.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IEY7"/>
    <dbReference type="SMR" id="B7IEY7"/>
    <dbReference type="STRING" id="484019.THA_143"/>
    <dbReference type="PRIDE" id="B7IEY7"/>
    <dbReference type="EnsemblBacteria" id="ACJ74651">
        <property type="protein sequence ID" value="ACJ74651"/>
        <property type="gene ID" value="THA_143"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_143"/>
    <dbReference type="eggNOG" id="COG0556">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009621_2_1_0"/>
    <dbReference type="OMA" id="EYVDRMV"/>
    <dbReference type="OrthoDB" id="95696at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00204">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004807">
        <property type="entry name" value="UvrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024759">
        <property type="entry name" value="UvrB_YAD/RRR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24029">
        <property type="entry name" value="PTHR24029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12344">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00631">
        <property type="entry name" value="uvrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0228">DNA excision</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0267">Excision nuclease</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_1000200560" description="UvrABC system protein B">
        <location>
            <begin position="1"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="23"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="1">
        <location>
            <begin position="426"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="1">
        <location>
            <begin position="620"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Beta-hairpin">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00204"/>
        </source>
    </evidence>
    <sequence length="661" mass="76444" checksum="6293E06D7475DEA4" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEZ1</accession>
    <name>B7IEZ1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103592.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_148"/>
    <dbReference type="EnsemblBacteria" id="ACJ74655">
        <property type="protein sequence ID" value="ACJ74655"/>
        <property type="gene ID" value="THA_148"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_148"/>
    <dbReference type="eggNOG" id="ENOG50339Y0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1757954_0_0_0"/>
    <dbReference type="OMA" id="TINTSKW"/>
    <dbReference type="OrthoDB" id="1510594at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="149" mass="17031" checksum="076594B58300CB6D" modified="2009-02-10" version="1">MEKYLIYILGTLLATIGLIFLSFYIAIFFFSPVIENIFSINMNISSALLIIAISFTLNGFFIGFYSISKDSWEYANVWIIISFLLSFISFLFQLYKLASLGPTWLGLEFFGINGNKIETMYIGMMLFLINLAILVICGILVFSRFRGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEZ3</accession>
    <name>B7IEZ3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription-repair-coupling factor</fullName>
            <shortName evidence="8">TRCF</shortName>
            <ecNumber evidence="8">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mfd</name>
        <name evidence="11" type="ordered locus">THA_150</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the C-terminal section; belongs to the helicase family. RecG subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the N-terminal section; belongs to the UvrB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579376.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_150"/>
    <dbReference type="EnsemblBacteria" id="ACJ74657">
        <property type="protein sequence ID" value="ACJ74657"/>
        <property type="gene ID" value="THA_150"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_150"/>
    <dbReference type="eggNOG" id="COG1197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005122_1_2_0"/>
    <dbReference type="OMA" id="IIESGIH"/>
    <dbReference type="OrthoDB" id="234717at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000716">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00969">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036101">
        <property type="entry name" value="CarD-like/TRCF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003711">
        <property type="entry name" value="CarD-like/TRCF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004576">
        <property type="entry name" value="Mfd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037235">
        <property type="entry name" value="TRCF-like_C_D7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005118">
        <property type="entry name" value="TRCF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02559">
        <property type="entry name" value="CarD_CdnL_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03461">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01058">
        <property type="entry name" value="CarD_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00982">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141259">
        <property type="entry name" value="SSF141259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143517">
        <property type="entry name" value="SSF143517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00580">
        <property type="entry name" value="mfd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="405"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="574"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ74657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="909" mass="104880" checksum="FD435886C04F91F3" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEZ6</accession>
    <name>PDXT_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase subunit PdxT</fullName>
            <ecNumber evidence="1">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pdx2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase glutaminase subunit</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxT</name>
        <name type="ordered locus">THA_153</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = H(+) + 3 H2O + L-glutamate + phosphate + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103609.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IEZ6"/>
    <dbReference type="SMR" id="B7IEZ6"/>
    <dbReference type="STRING" id="484019.THA_153"/>
    <dbReference type="MEROPS" id="C26.A32"/>
    <dbReference type="EnsemblBacteria" id="ACJ74660">
        <property type="protein sequence ID" value="ACJ74660"/>
        <property type="gene ID" value="THA_153"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_153"/>
    <dbReference type="eggNOG" id="COG0311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069674_2_0_0"/>
    <dbReference type="OMA" id="VFIRAPI"/>
    <dbReference type="OrthoDB" id="1628378at2"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PTHR31559"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000185908" description="Pyridoxal 5'-phosphate synthase subunit PdxT">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="46"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="133"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01615"/>
        </source>
    </evidence>
    <sequence length="188" mass="21354" checksum="1A494C891BD56F64" modified="2009-02-10" version="1">MKIGVSGIQGDFREHKVMLEKLGVDVKVVRKPEELDEVDGLVIPGGESTTMIRIMKMVNLYEKLKQKIKEGFPVFGTCAGMILLSKEVVNFPQDSLGVIDIKVERNAYGRQVDSFEEQIEVKGFEKTFNAIFIRAPKVVDYGENVEVLATYMDSPVLLRQNNVLVASFHPELTEDTRIHEYFLNMVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF51</accession>
    <name>B7IF51_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exodeoxyribonuclease VII large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579417.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_210"/>
    <dbReference type="EnsemblBacteria" id="ACJ74715">
        <property type="protein sequence ID" value="ACJ74715"/>
        <property type="gene ID" value="THA_210"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_210"/>
    <dbReference type="eggNOG" id="COG1570">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023625_4_2_0"/>
    <dbReference type="OMA" id="WPAVRFE"/>
    <dbReference type="OrthoDB" id="1371775at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009318">
        <property type="term" value="C:exodeoxyribonuclease VII complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008855">
        <property type="term" value="F:exodeoxyribonuclease VII activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003753">
        <property type="entry name" value="Exonuc_VII_L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020579">
        <property type="entry name" value="Exonuc_VII_lsu_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30008">
        <property type="entry name" value="PTHR30008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02601">
        <property type="entry name" value="Exonuc_VII_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuc_VII_L" evidence="1">
        <location>
            <begin position="151"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="481" mass="55566" checksum="9141A28C593F7992" modified="2009-02-10" version="1">MAGIKEFKDLIELNEYVTKKIEMTGLTSETFRFYADVVRANNHSTGLYIDVSQPYTAKNGTRNIEITVYVPRYLAPKILEVIKVSNVKELVGKKWIFQGRLSFFRDRMSFTFYADTIAPMGESEIEKRRKEILKELEVRNLLMKEKHDLSELPPIKKIAIITSKSAAGYEDFLKNLTVHYLYRPIVHLYESPMQGAQTASGIILALNRIRKSNIDYDVVVIARGGGARSDLMYFDDLSLGIEIAKFNEYCPILSGIGHERDFTIPDYVAWKRFATPTEVARAISKQIEDNVKKLDDSYNDLRILLSNVFKIYERTVELGLIDYMKKVIGSDFIKIVKDLDETYEKIENFVSYKINDSSQRLSEDFLRFMSNSLENKLKSKKDSVENFEKILERDISILLSNKETMLNETFQELLKREEFAPLLFGGALVMKSGHFVKSIRQMKIGDKLDLYLKDGKVKTKVVTEKKIKKEELYGGHTLFRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF54</accession>
    <name>B7IF54_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell division protein FtsA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579420.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_213"/>
    <dbReference type="EnsemblBacteria" id="ACJ74718">
        <property type="protein sequence ID" value="ACJ74718"/>
        <property type="gene ID" value="THA_213"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_213"/>
    <dbReference type="eggNOG" id="COG0849">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010661_1_0_0"/>
    <dbReference type="OMA" id="MVPMAGD"/>
    <dbReference type="OrthoDB" id="1044637at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003494">
        <property type="entry name" value="SHS2_FtsA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00842">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FtsA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="695" mass="77293" checksum="DC217154FBA9FE18" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF55</accession>
    <name>B7IF55_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endonuclease MutS2</fullName>
            <ecNumber evidence="7">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mutS2</name>
        <name evidence="10" type="ordered locus">THA_214</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Endonuclease that is involved in the suppression of homologous recombination and may therefore have a key role in the control of bacterial genetic diversity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DNA mismatch repair MutS family. MutS2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579421.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_214"/>
    <dbReference type="EnsemblBacteria" id="ACJ74719">
        <property type="protein sequence ID" value="ACJ74719"/>
        <property type="gene ID" value="THA_214"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_214"/>
    <dbReference type="eggNOG" id="COG1193">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011252_2_1_0"/>
    <dbReference type="OMA" id="IHAIIND"/>
    <dbReference type="OrthoDB" id="256392at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00092">
        <property type="entry name" value="MutS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005747">
        <property type="entry name" value="MutS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="PTHR11361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF14">
        <property type="entry name" value="PTHR11361:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005814">
        <property type="entry name" value="MutS_YshD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SSF160443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="SSF48334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01069">
        <property type="entry name" value="mutS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="9">
        <location>
            <begin position="691"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="499"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="319"/>
            <end position="326"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="765" mass="88187" checksum="D2C8FFE5D4E755CD" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF65</accession>
    <name>DNAA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chromosomal replication initiator protein DnaA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaA</name>
        <name type="ordered locus">THA_224</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DnaA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103763.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IF65"/>
    <dbReference type="SMR" id="B7IF65"/>
    <dbReference type="STRING" id="484019.THA_224"/>
    <dbReference type="EnsemblBacteria" id="ACJ74729">
        <property type="protein sequence ID" value="ACJ74729"/>
        <property type="gene ID" value="THA_224"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_224"/>
    <dbReference type="eggNOG" id="COG0593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026910_3_2_0"/>
    <dbReference type="OMA" id="REFNPLF"/>
    <dbReference type="OrthoDB" id="219876at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06571">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1750.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00377">
        <property type="entry name" value="DnaA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001957">
        <property type="entry name" value="Chromosome_initiator_DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020591">
        <property type="entry name" value="Chromosome_initiator_DnaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013317">
        <property type="entry name" value="DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013159">
        <property type="entry name" value="DnaA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024633">
        <property type="entry name" value="DnaA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038454">
        <property type="entry name" value="DnaA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30050:SF2">
        <property type="entry name" value="PTHR30050:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00308">
        <property type="entry name" value="Bac_DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08299">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11638">
        <property type="entry name" value="DnaA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00051">
        <property type="entry name" value="DNAA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00760">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00362">
        <property type="entry name" value="DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000122028" description="Chromosomal replication initiator protein DnaA">
        <location>
            <begin position="1"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="139"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00377"/>
        </source>
    </evidence>
    <sequence length="438" mass="50319" checksum="7B408CD98E74B4D6" modified="2009-02-10" version="1">MKDNILSALKEKISRQNWESWFLDFNVKKIEDKHVVFEVGNIFIKDRLEQKFSKIISKVVKESLGKDATFEIVYKEIDITQENEEKGPLVRKRPLLITPLNPKYTFENFVVGNFNRFAYNVFLEASKKPGYYNPIFLYSGVGLGKTHLAQALGNFLLENDPDLKVAYLTSEDFMNEMFYAIKNGTTEEFREKYRKKADILIIDDIQFLIGIKSAQVELFHTFNAIHEAGKQIIICSDRTPQELKDFHSRMISRFQMGLLVKIENPTKEDLFKIGKKISKLKGVEINDEIIDYISSIYDNPRLIHGAILKLIAYKNLYGSLNLSIAQSILTNPSKPPKALEEKLIEILSDIFECSPEEILSSKRTKNISYARKVGMYYAAKKLNLSTRDVGKVFNKSHSSVVQNISQVEKLIKEGNIIIKNYLKQIDKATKNFAQGESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF85</accession>
    <name>B7IF85_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin reductase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579440.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_247"/>
    <dbReference type="EnsemblBacteria" id="ACJ74749">
        <property type="protein sequence ID" value="ACJ74749"/>
        <property type="gene ID" value="THA_247"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_247"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_3_0"/>
    <dbReference type="OMA" id="RNAWRVE"/>
    <dbReference type="OrthoDB" id="692968at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="2"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="262" mass="29462" checksum="A18A10D80E595081" modified="2009-02-10" version="1">MQVGIIGAGPAGISAAVFLKRYGIDVALFEKKRIGGLINNAWRVENIPFFQPLPGKKIVENMKSYLELYNVNIIFEEVKEVYDKRIITEKDTYYFDYIIVASGTVPNRIEELEGKRVYYEFLDLPDNIESVAIYGAGDVAFDGAIQAKILRKNVHIFNRSNRIKALSRLVNIASSLGVIYHESEPIKIVENKENYVIIHTKKSVYKFDALLLATGRKVDLSFLKTDKVFLIGDVAHPNFRQASIAMGDGIKIAMKIIGEGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF90</accession>
    <name>B7IF90_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thiamine pyrophosphokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_252</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103812.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_252"/>
    <dbReference type="EnsemblBacteria" id="ACJ74754">
        <property type="protein sequence ID" value="ACJ74754"/>
        <property type="gene ID" value="THA_252"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_252"/>
    <dbReference type="eggNOG" id="COG1564">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044237_1_1_0"/>
    <dbReference type="OMA" id="ITYCPEG"/>
    <dbReference type="OrthoDB" id="1628990at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030975">
        <property type="term" value="F:thiamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07995">
        <property type="entry name" value="TPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006282">
        <property type="entry name" value="Thi_PPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007373">
        <property type="entry name" value="Thiamin_PyroPKinase_B1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007371">
        <property type="entry name" value="TPK_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04265">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04263">
        <property type="entry name" value="TPK_catalytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00983">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="SSF63999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01378">
        <property type="entry name" value="thi_PPkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TPK_B1_binding" evidence="5">
        <location>
            <begin position="132"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="205" mass="23387" checksum="39AE9AA1C9BC89CC" modified="2009-02-10" version="1">MKATIVLNGNIDETLVLNTNHIIAVDGGAKLLKKRNILPNIVIGDLDSIDNETIDFLKKQSVKIIEYPKEKDETDCELAIKYALENGFKEIEIINFQGERIDMIFALYGLLKKFDANIYLNSEKLESAILKENNTLEKEVKIGEIWSFIPLCNSTFMIEGFKYPFNGNMGIENPIGVSNETTAEKIKIHVEKGEVIYFRWKKKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFD9</accession>
    <name>B7IFD9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="4 6">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nadE1</name>
        <name evidence="4" type="synonym">nadE</name>
        <name evidence="10" type="ordered locus">THA_303</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 7">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="4 6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_303"/>
    <dbReference type="EnsemblBacteria" id="ACJ74803">
        <property type="protein sequence ID" value="ACJ74803"/>
        <property type="gene ID" value="THA_303"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_303"/>
    <dbReference type="eggNOG" id="COG0171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059327_0_0_0"/>
    <dbReference type="OMA" id="YFHADRL"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00193">
        <property type="entry name" value="NadE_ammonia_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022926">
        <property type="entry name" value="NH(3)-dep_NAD(+)_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="PTHR23090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_synthase" evidence="9">
        <location>
            <begin position="6"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_synthase" evidence="9">
        <location>
            <begin position="146"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="290" mass="33646" checksum="658AFC8F015A2BB9" modified="2009-02-10" version="1">MTKKSEKIVNFLRETVAQYNFRGLVLGVSGGLDSAVVLALLVKAFDRDKIKCFILPERDSPKDSVKDAVFVCKYFGVEYEIKNITKILRALGIYKYYPPAFFVPWKVKENFAKKRWQKYKEKGNPFEFDIEGIEDEEFLKGISYYRAKHRVRMVYLYKEAERRNYAVVGTTNKTEFLTGLYVKWGDDSTDIEPILHLYKTQVYELAKELNVPEKIIMKPASPDLIPGIGDEEIFGLDYSTLDRILDKLLNNKSLDGEDPEKVKLVKRLYQIGKKRNSLRNVSMVDDLYGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFG9</accession>
    <name>B7IFG9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_333</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579505.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_333"/>
    <dbReference type="EnsemblBacteria" id="ACJ74833">
        <property type="protein sequence ID" value="ACJ74833"/>
        <property type="gene ID" value="THA_333"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_333"/>
    <dbReference type="eggNOG" id="COG1561">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076609_1_0_0"/>
    <dbReference type="OMA" id="EIKSVNH"/>
    <dbReference type="OrthoDB" id="1509828at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005229">
        <property type="entry name" value="CHP00255"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013551">
        <property type="entry name" value="DUF1732"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013527">
        <property type="entry name" value="YicC-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30636">
        <property type="entry name" value="PTHR30636"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08340">
        <property type="entry name" value="DUF1732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03755">
        <property type="entry name" value="YicC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00255">
        <property type="entry name" value="TIGR00255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YicC_N" evidence="2">
        <location>
            <begin position="2"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1732" evidence="3">
        <location>
            <begin position="209"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="208"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="291" mass="33633" checksum="19CFCE0BD7864F61" modified="2009-02-10" version="1">MLKSMTGYAKVEKFSEKFRVSCEVKSLNSKGLDINVSLPYYLSSKEIEITKIISSFLTRGKVNVRINVKFLTPVNLSIDYVMAKSYYDTLEGVRESLGIQNSIVLGDLLNFREIFRGDIEDEVVEELWNFTKEVLSSSLEMLVEERKKEGQKICKDIENMVNKIEEITLNIEKVASDMKEEIAKKLRESVEEILPENVEMDTNQFETAVALIADKADIREEIERLKSHIDRMKELLLKDEPVGMLFNFLTQEVHREFNTILSKSRMLEISNLALEGKYINSQLKEQIQNVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFK1</accession>
    <name>B7IFK1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ABC-type multidrug/protein/lipid transport system, ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">THA_371</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_371"/>
    <dbReference type="EnsemblBacteria" id="ACJ74865">
        <property type="protein sequence ID" value="ACJ74865"/>
        <property type="gene ID" value="THA_371"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_371"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_4_0"/>
    <dbReference type="OMA" id="LGIMLWG"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="289"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="49"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="376"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="409"/>
            <end position="416"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ74865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="617" mass="69184" checksum="F7D5D596D1DCABBE" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFK2</accession>
    <name>B7IFK2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_372</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579530.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_372"/>
    <dbReference type="EnsemblBacteria" id="ACJ74866">
        <property type="protein sequence ID" value="ACJ74866"/>
        <property type="gene ID" value="THA_372"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_372"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_9_0"/>
    <dbReference type="OMA" id="MSVMMAT"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="16"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="331"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="365"/>
            <end position="372"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="574" mass="64282" checksum="8EB25D1C3996A421" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFM6</accession>
    <name>RL25_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L25</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">General stress protein CTC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplY</name>
        <name evidence="1" type="synonym">ctc</name>
        <name type="ordered locus">THA_397</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA. Binds to the 5S rRNA independently of L5 and L18.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL25 family. CTC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104129.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFM6"/>
    <dbReference type="SMR" id="B7IFM6"/>
    <dbReference type="STRING" id="484019.THA_397"/>
    <dbReference type="EnsemblBacteria" id="ACJ74890">
        <property type="protein sequence ID" value="ACJ74890"/>
        <property type="gene ID" value="THA_397"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_397"/>
    <dbReference type="eggNOG" id="COG1825">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075939_2_1_0"/>
    <dbReference type="OMA" id="HVDFYEV"/>
    <dbReference type="OrthoDB" id="1673077at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00495">
        <property type="entry name" value="Ribosomal_L25_TL5_CTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01334">
        <property type="entry name" value="Ribosomal_L25_CTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_L25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029751">
        <property type="entry name" value="Ribosomal_L25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_L25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020057">
        <property type="entry name" value="Ribosomal_L25_b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037121">
        <property type="entry name" value="Ribosomal_L25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001021">
        <property type="entry name" value="Ribosomal_L25_long"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01386">
        <property type="entry name" value="Ribosomal_L25p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14693">
        <property type="entry name" value="Ribosomal_TL5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="SSF50715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00731">
        <property type="entry name" value="bL25_bact_ctc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000142555" description="50S ribosomal protein L25">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="217" mass="24355" checksum="58EE8C95270993FA" modified="2009-02-10" version="1">MEKHTLEALVRSVVGKKRAVRRLRRQGFVPGVVYGPDVEPLSISIKRSNLIKLFHEVTEASIISLTVKDENGKEVFSHDVFIKNVQYDKLTDEVKHVDFYAPEKGHKMKINLPLEFVGKAKGEEKGGVLEIHHHELPVETLPNAVVEKLEIDVSNLDLGQALYVKDLKLPEGMEAELDEEEIIAIVSTPSGLEVEEETGEEESAEPEVIEKGKKEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFN0</accession>
    <name>B7IFN0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579549.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_401"/>
    <dbReference type="EnsemblBacteria" id="ACJ74894">
        <property type="protein sequence ID" value="ACJ74894"/>
        <property type="gene ID" value="THA_401"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_401"/>
    <dbReference type="eggNOG" id="COG2182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_715583_0_0_0"/>
    <dbReference type="OMA" id="WFSTFLM"/>
    <dbReference type="OrthoDB" id="1287217at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="407" mass="47882" checksum="98CFC8C58F58D1F8" modified="2009-02-10" version="1">MKKAFVVIFIISSVLLFSYIVSPKNFPDGKYRVIELIPPFEKIEAISLISNDVKENIALYDLIEIKNGNFYFKGKKFTNMEVKAEFLEKLQREVWLSWEGINDIKEYIEKYSKMHDLNIKVLEVPQISSKLITMSKSNVKLPDVIMTSAFDYPTYKELNIVKGKLYNYYYDTQIVYVQKDINIVPKSWNLKTVEKIVQDTNKKIAINPISAYWFSTFLMGYGKIPLISDAFILTDENTINAFNVVKKWYENNYFDFLNKQAQIASFVNKKVALMFQGSFLLPIISEKMEDFYILPLPKPLVPFKDYKAFVSTKDGDEHFTYWLYLLLNNPDFKKYFADKYTKFFDDYIPEGKFKNVFESTQKVAQSIPFDKRYVKFHKNVSDILKLMLFNKISLQDGLKKLEEIVNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFN4</accession>
    <name>ENO_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enolase</fullName>
            <ecNumber evidence="1">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eno</name>
        <name type="ordered locus">THA_405</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <text evidence="1">Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579553.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFN4"/>
    <dbReference type="SMR" id="B7IFN4"/>
    <dbReference type="STRING" id="484019.THA_405"/>
    <dbReference type="PRIDE" id="B7IFN4"/>
    <dbReference type="EnsemblBacteria" id="ACJ74898">
        <property type="protein sequence ID" value="ACJ74898"/>
        <property type="gene ID" value="THA_405"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_405"/>
    <dbReference type="eggNOG" id="COG0148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031223_2_1_0"/>
    <dbReference type="OMA" id="EFMIIPV"/>
    <dbReference type="OrthoDB" id="533698at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="PTHR11902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <feature type="chain" id="PRO_1000119581" description="Enolase">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent; in inhibited form" evidence="1">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="365"/>
            <end position="368"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <sequence length="429" mass="47029" checksum="3725683278F01F84" modified="2009-02-10" version="1">MYIEIIDVFAREVLDSRGNPTVEVEVMLEDGSIGRAIVPSGASTGKFEALELRDGDKKRYLGKGVLKAVENVNELIAPKLLGLNAYDQVYIDNVLLELDGTENKSKLGANAILGVSMAVARAAANSLQLPLYKYLGGVNAKVLPVPLMNVINGGAHADNNLDIQEFMIVPAGAPSFREALRYGAETFHALKKILKEAGHVTAVGDEGGFAPNLKNNEEAIQVLIQAIQSAGYEPGKDIFIALDVAASEFYNEETGKYFIDGSEKTVDELIEYYKSLIEKYPIISIEDPFDQEDWEAYQKFNKEVGNKVQIVGDDLYVTNVKRLQKGIELKASNSILIKLNQIGSVTETLNAIELAKTNNMTNVISHRSGETEDTFIADLAVATNAGLIKTGSLSRSERIAKYNQLLRIEEELGDVAQYRGLDAFYSIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFP8</accession>
    <name>B7IFP8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">LysM domain/M23/M37 peptidase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579566.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_419"/>
    <dbReference type="MEROPS" id="M23.009"/>
    <dbReference type="EnsemblBacteria" id="ACJ74912">
        <property type="protein sequence ID" value="ACJ74912"/>
        <property type="gene ID" value="THA_419"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_419"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029425_7_3_0"/>
    <dbReference type="OMA" id="LFEINGC"/>
    <dbReference type="OrthoDB" id="1891666at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="1">
        <location>
            <begin position="24"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="1">
        <location>
            <begin position="73"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="273" mass="30542" checksum="A390613E7E5B59A9" modified="2009-02-10" version="1">MNKKVLAIFFFLIAASLFANYYILNYYIQPGDTLYLISKEFNVSPSVILDWNDIDPYHLKVGQSIKIPQPNGIIYEVKQGDTLYDISLRFFTTVDAIKKANNLLSSFIYVGQKLFIPIDYVGVAFNVYDKSFIWPVYGKISSTYGSRIHPIYQKRTFHTGLDIAAPEGSPIFSATNGVVTHAGEYGGYGLAVIVKYGNYEIVYGHMSKVSVYKGQTVRKGELLGRVGSTGISTGPHLHFEVRINGKHTNPVVFLPSYGRMYVLKDQGLYLGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFR5</accession>
    <name>B7IFR5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Radical SAM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579580.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_438"/>
    <dbReference type="EnsemblBacteria" id="ACJ74929">
        <property type="protein sequence ID" value="ACJ74929"/>
        <property type="gene ID" value="THA_438"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_438"/>
    <dbReference type="eggNOG" id="COG1032">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011543_3_3_0"/>
    <dbReference type="OMA" id="PESYNWS"/>
    <dbReference type="OrthoDB" id="970297at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045784">
        <property type="entry name" value="Radical_SAM_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19864">
        <property type="entry name" value="Radical_SAM_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="215"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="460" mass="54066" checksum="68030E07A3BC73A3" modified="2009-02-10" version="1">MRRPRKSDDFKEYSYVKKFSDSEIRLNSINGNRGVALIFPNNYTVAASSLSWSWVQQLFWEYNIPVERYFYEKWFKKFYSIESFTPLDENKVFLFSFHFEPDIENIVDILKKQGIPILKNQRNENHPTIIIGGAITFFNLELVKPIADVIVIGDLEDKVEQISEGINILLKDKESGLKFLDKIDNVIVNEQKNYTLSNFDISKKLPASHIITRFSEFGEKRLIEIGRGCIRRCKFCVMGNTKKPVKFVKPELIKDLLKDEKYPIGIISATITDYPWLDELLDILEYYEIKFSVSSMRADGITKRLLRLLKESGQNTYTIAPEGISQKIRDVMLKDLNEEELLTALEMGRMEDFSNVKLYYIVGFDEEDNEDFNELKSFIDKVKKLGYKSITLSINPLIPKKNTPFYGRKFINERRYNEIRKWMYENLKGVKLHFESYKLSKKQNLYNNISLEELINLFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFS8</accession>
    <name>B7IFS8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_451</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579590.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_451"/>
    <dbReference type="EnsemblBacteria" id="ACJ74942">
        <property type="protein sequence ID" value="ACJ74942"/>
        <property type="gene ID" value="THA_451"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_451"/>
    <dbReference type="eggNOG" id="ENOG502ZASC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_537197_0_0_0"/>
    <dbReference type="OMA" id="RWKWFWD"/>
    <dbReference type="OrthoDB" id="310145at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="471" mass="55241" checksum="ABB4C69E931E114C" modified="2009-02-10" version="1">MLKQKFIIFLLLFSSIYFSEFIFYSSNNFENNFENKFSINIMCSFSNWSNGFVVFHGDGNGYPTNPAENVFYLFDNIKPFFEISLFYNFQNLVLYTNIPFQKEIISKVKDPSTNFFLVDGKPTFDMNGPENFLLGYISDNIFIGIGRFPLHWGDSKYPISISDTTFQDNITFSTKNNFFKYTYHLISSYPLLSSYEQEVQRKYFEKHTPSTNFFEPVKTIAAHRFDFFIDNFRIGIGEINVVGGKIPDIIDINPLMFFHNTYGEGYSNVLSSIDFNVKVSNISIYGEFCLDDINGPTEVGTNYKPDAYGYNFGMFLDYKPFNVWIEYDFTSEWMYITNYLPYLRVNVRHFYIDNNSFPSRSLMDYPLGFRYGPDATMISLGFSFSKSNFKVTTEFNLLVKGMVIDDSNGKIRWKWFWDSWSGNVNPIPESSVINTFDQVYNIFSFEFSYKFLSFKSLFIDKNYFISLQVNF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFU5</accession>
    <name>MURD_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylmuramoylalanine--D-glutamate ligase</fullName>
            <ecNumber evidence="1">6.3.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">D-glutamic acid-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murD</name>
        <name type="ordered locus">THA_468</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + D-glutamate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16429"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29986"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83898"/>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurCDEF family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.9" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104249.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFU5"/>
    <dbReference type="SMR" id="B7IFU5"/>
    <dbReference type="STRING" id="484019.THA_468"/>
    <dbReference type="EnsemblBacteria" id="ACJ74959">
        <property type="protein sequence ID" value="ACJ74959"/>
        <property type="gene ID" value="THA_468"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_468"/>
    <dbReference type="eggNOG" id="COG0771">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032540_0_1_0"/>
    <dbReference type="OMA" id="CNFENYL"/>
    <dbReference type="OrthoDB" id="1093223at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008764">
        <property type="term" value="F:UDP-N-acetylmuramoylalanine-D-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00639">
        <property type="entry name" value="MurD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005762">
        <property type="entry name" value="MurD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01087">
        <property type="entry name" value="murD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000130878" description="UDP-N-acetylmuramoylalanine--D-glutamate ligase">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="112"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00639"/>
        </source>
    </evidence>
    <sequence length="423" mass="48274" checksum="35AA9FAF02AF9D74" modified="2009-02-10" version="1">MKYTLLGFGISNKEILKYLLKKGERVFVSEGKKLSEVDKEFLNSFGVDFEENGHTEKVLDSDVILVSPGIHFENDIIKKAKENNIKIDTEISFCLKEFEKINWFPYVIAVTGSVGKSTTVSMIQHVLNKYKRTLLAGNIGIPIAKLLNDDLRADYLVLEVSSFQLFWSDKFKPNISSILNIYPNHLNWHPEMSHYINSKFKITLSQDENDFFVYNPNDEYIVKNLGKVKAKKVPFKYDFEIHQLPEHLRYKQTIENVAAAKTIVEVAGFEFNIDFLEDFEKLPHRMEYVTEINGVKFFNDSKATNAAAVIRAVENFDGKLHLIMAGIGKNEDYTLLRKVLKNSVKTVALVGPIAKDVKPYLDGINFMECSSINEAVNQLFRIANPGDVIMLSPGGASFDAFKNFEERGEYFKQLVFQLKEGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFU8</accession>
    <name>B7IFU8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="6">2.1.1.182</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">16S rRNA dimethyladenosine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">16S rRNA dimethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rsmA</name>
        <name evidence="6 9" type="synonym">ksgA</name>
        <name evidence="9" type="ordered locus">THA_471</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>adenosine(1518)/adenosine(1519) in 16S rRNA + 4 S-adenosyl-L-methionine = 4 H(+) + N(6)-dimethyladenosine(1518)/N(6)-dimethyladenosine(1519) in 16S rRNA + 4 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:19609"/>
            <dbReference type="Rhea" id="RHEA-COMP:10232"/>
            <dbReference type="Rhea" id="RHEA-COMP:10233"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.182"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.182" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579600.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_471"/>
    <dbReference type="EnsemblBacteria" id="ACJ74962">
        <property type="protein sequence ID" value="ACJ74962"/>
        <property type="gene ID" value="THA_471"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_471"/>
    <dbReference type="eggNOG" id="COG0030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041220_0_0_0"/>
    <dbReference type="OMA" id="KEEEPYF"/>
    <dbReference type="OrthoDB" id="2030110at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052908">
        <property type="term" value="F:16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="PTHR11727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="rADc" evidence="8">
        <location>
            <begin position="28"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="261" mass="30188" checksum="0F1F5B873BB318F3" modified="2009-02-10" version="1">MKIADYLRQYKLNLKKSLGQNFLTNLEIAKKIVESANVQTGETVLEIGPGAGTITEFLIKKGANVVAVEIDKRLTPILDRFNEFENFKIYYQDFLKFDMKLLGENFKVVSNIPYSITGMIIKKILFSKFSTAVLMVQKEVGDRLLAKPGEDRSFLTVVVQTFTDIEKVLHVSKGNFVPPPQVDSVVLKFTRKENIYEKYNIEEFWTFVSKCFEKKRKTLLNNLKGFVKNLDCFSEFDLKLRPQQLSNDDFLKLFEKYKGCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFY9</accession>
    <name>B7IFY9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">S1 RNA binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_514</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579627.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_514"/>
    <dbReference type="EnsemblBacteria" id="ACJ75003">
        <property type="protein sequence ID" value="ACJ75003"/>
        <property type="gene ID" value="THA_514"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_514"/>
    <dbReference type="eggNOG" id="COG2183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009833_0_2_0"/>
    <dbReference type="OMA" id="RWAWRTR"/>
    <dbReference type="OrthoDB" id="120764at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3500.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041692">
        <property type="entry name" value="HHH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023319">
        <property type="entry name" value="Tex-like_HTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018974">
        <property type="entry name" value="Tex-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032639">
        <property type="entry name" value="Tex_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17674">
        <property type="entry name" value="HHH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09371">
        <property type="entry name" value="Tex_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16921">
        <property type="entry name" value="Tex_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="614"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="43"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="675" mass="78260" checksum="A14D62E2ECC2AD72" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG07</accession>
    <name>B7IG07_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flagellar hook-associated protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">flgL</name>
        <name evidence="2" type="ordered locus">THA_533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579636.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_533"/>
    <dbReference type="EnsemblBacteria" id="ACJ75021">
        <property type="protein sequence ID" value="ACJ75021"/>
        <property type="gene ID" value="THA_533"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_533"/>
    <dbReference type="eggNOG" id="COG1344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024437_2_0_0"/>
    <dbReference type="OMA" id="MRVTQGM"/>
    <dbReference type="OrthoDB" id="1788350at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009424">
        <property type="term" value="C:bacterial-type flagellum hook"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013384">
        <property type="entry name" value="Flagell_FlgL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001492">
        <property type="entry name" value="Flagellin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001029">
        <property type="entry name" value="Flagellin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42792">
        <property type="entry name" value="PTHR42792"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00669">
        <property type="entry name" value="Flagellin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02550">
        <property type="entry name" value="flagell_flgL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flagellin_N" evidence="1">
        <location>
            <begin position="8"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="295" mass="32998" checksum="F728B759320C38A7" modified="2009-02-10" version="1">MRITNGMINERTLFNIQHSLYRISKLHDKLSSGKEVSYPSDDAVVATRASNISSRMRELKQFKRNVEHTQNFVNIYDSTIQELTNVYHRIKELIVRGANGTNDAAEREAIASELEKLKEHLEDIANTQLGGEYIFGGARSDLKPVENGKIQTPPDANIKRKVNAMGFTIEYGVTVYDVFKLENGKDAFTVIDDAINALREGNSLKLSNITLKEVNLLESSTMEVFAQIGANSRTLELVSSRLTDIDTFMTEYLSKEQDADLTKVLTDLSMQQSVLQAALKSAAQVLQKTLVDFVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG21</accession>
    <name>B7IG21_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hit family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_547</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100603.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_547"/>
    <dbReference type="EnsemblBacteria" id="ACJ75035">
        <property type="protein sequence ID" value="ACJ75035"/>
        <property type="gene ID" value="THA_547"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_547"/>
    <dbReference type="eggNOG" id="COG0537">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_8_1_0"/>
    <dbReference type="OMA" id="SDCLFCK"/>
    <dbReference type="OrthoDB" id="1934382at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="PTHR23089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="5"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="1">
        <location>
            <begin position="96"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="111" mass="13109" checksum="92CF9E9554288B8B" modified="2009-02-10" version="1">MENCVFCKIINNEIPSEKLYEDEDFIVIKDINPVAPIHLLVIYKKHVPVIHELSEDDQRRFWKIFNIIKKLSEELNFKEYRIVQNNGKQAGQEIPHIHFHVIAGRKFGKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG52</accession>
    <name>B7IG52_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_579</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579657.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_579"/>
    <dbReference type="EnsemblBacteria" id="ACJ75066">
        <property type="protein sequence ID" value="ACJ75066"/>
        <property type="gene ID" value="THA_579"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_579"/>
    <dbReference type="eggNOG" id="ENOG502ZC2Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105443_0_0_0"/>
    <dbReference type="OMA" id="CALVKND"/>
    <dbReference type="OrthoDB" id="1701025at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="199" mass="21932" checksum="4A4E5529030664BF" modified="2009-02-10" version="1">MINVSNIEEVLTKIPGIQAAKAVVEDEEIVELHVVADDEKLPKQIVRDIETVLFASLGIKIDRKVISIAQLNSKIESSKVLPYRLEKLDVKDEGKNIKVSVEIVHGQEKMSGEFSGPKTSRNIPIVIGNAVLNALENIHDFVISVDDIAEVSLAGKNFLITHLTKEYKNVEESIIGAAPIEKDKNTAIAESVLDAFRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG84</accession>
    <name>B7IG84_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Autolysin response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_612</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579681.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_612"/>
    <dbReference type="EnsemblBacteria" id="ACJ75098">
        <property type="protein sequence ID" value="ACJ75098"/>
        <property type="gene ID" value="THA_612"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_612"/>
    <dbReference type="eggNOG" id="COG3279">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_14_1_0"/>
    <dbReference type="OMA" id="IQAFEEH"/>
    <dbReference type="OrthoDB" id="1958442at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007492">
        <property type="entry name" value="LytTR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04397">
        <property type="entry name" value="LytTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00850">
        <property type="entry name" value="LytTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50930">
        <property type="entry name" value="HTH_LYTTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="3"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="HTH LytTR-type" evidence="3">
        <location>
            <begin position="125"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="227" mass="26685" checksum="D00B020D0F766006" modified="2009-02-10" version="1">MIKVAIIDDEYYARESLKDLINEMTDFEIVGCFESVEEFLKNKKNKKTDVIFLDIEMPSINGIKASKCLDKYEIVFVTAYSEYAVEAFEVNALDYLTKPVSEIRFLETIKRIEEKLRTKNIINTIAAENNNEIIFLSFDDILYFEYFEKNIIAITEDNEFVIKHYKSLGSLEKDLPQNFVRIHKSYIINLNFIEKFIKEPISLEMKNKKLLPIGKTHLKEVKKILKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGB0</accession>
    <name>B7IGB0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_646</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579699.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_646"/>
    <dbReference type="EnsemblBacteria" id="ACJ75124">
        <property type="protein sequence ID" value="ACJ75124"/>
        <property type="gene ID" value="THA_646"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_646"/>
    <dbReference type="eggNOG" id="COG1592">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095256_1_0_0"/>
    <dbReference type="OMA" id="AYAEFVH"/>
    <dbReference type="OrthoDB" id="1681849at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01041">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="2">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin-like" evidence="1">
        <location>
            <begin position="135"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="168" mass="19287" checksum="884C1351EAD32F8B" modified="2009-02-10" version="1">MREMTKQFLEAAFAGESMAHMKYLIFAEEAEKKGLLKLSKLFKAIAYAEYVHAKNHFKVLKKLSDDMKDNVQSCIDGETFEIEEMYPVYKNAAEFQDEKDAVRSTHFALEAEKIHADMYKKAKELVEKGEDYPAEKIYICPVCGYTVEDEAPEKCPVCGAPKDKFVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGF4</accession>
    <name>B7IGF4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Na(+)-translocating NADH-quinone reductase subunit c (Na(+)-translocating nqr subunit c) (Na(+)-nqr subunit c), putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">THA_697</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104580.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_697"/>
    <dbReference type="EnsemblBacteria" id="ACJ75168">
        <property type="protein sequence ID" value="ACJ75168"/>
        <property type="gene ID" value="THA_697"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_697"/>
    <dbReference type="eggNOG" id="COG4659">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077882_0_2_0"/>
    <dbReference type="OMA" id="LWGTITG"/>
    <dbReference type="OrthoDB" id="730368at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016655">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007329">
        <property type="entry name" value="FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010204">
        <property type="entry name" value="NqrC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37838">
        <property type="entry name" value="PTHR37838"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04205">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00900">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="FMN_bind" evidence="12">
        <location>
            <begin position="97"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ75168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="200" mass="22578" checksum="EDEEFD4440844697" modified="2009-02-10" version="1">MKENKIYTIIFTFVISFLFVLILSIINSMTYDKVLENQNFSKIRAILNSMGIEYKDEKDAIKIFNSKIDQIEIGGLKFYTTTINGEKIYSYIFSGSGLWGTITGVISVNENVDRIIGIDIISQNETPGLGGRIEEDWFKQQFKGELVKDKIKIKVSGASSDFNKENSSVDAITGATLTSRFFINIINDSLEKIRNALRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGF7</accession>
    <name>B7IGF7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">4Fe-4S binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_700</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_700"/>
    <dbReference type="EnsemblBacteria" id="ACJ75171">
        <property type="protein sequence ID" value="ACJ75171"/>
        <property type="gene ID" value="THA_700"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_700"/>
    <dbReference type="eggNOG" id="COG1145">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1774205_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="148" mass="16868" checksum="FA8F754124637946" modified="2009-02-10" version="1">MPLNCFSGMKEQEIKEILRNLPNKIDDIVDNLLKEKRKVLKPTLKGLILSKFSIFEKIFSKLFAKGYKVNDNCNLCGFCIKNCPTNNISIKNGKVHFGFNCALCLKCIYGCPQGAIIQRMFPFMIIKDGYKLENYLNKFGGDLNGKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGG9</accession>
    <name>B7IGG9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Bacterioferritin comigratory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_712</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579747.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_712"/>
    <dbReference type="EnsemblBacteria" id="ACJ75183">
        <property type="protein sequence ID" value="ACJ75183"/>
        <property type="gene ID" value="THA_712"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_712"/>
    <dbReference type="eggNOG" id="COG1225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_1_0"/>
    <dbReference type="OMA" id="RVVVYFY"/>
    <dbReference type="OrthoDB" id="1996723at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="3">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="150" mass="17368" checksum="5FFE38CC62BD9474" modified="2009-02-10" version="1">MEKAKNFCLPDKNGKMHCLEDFKGKWVVLYFYPKDNTSGCTREAKDFSQLKEEFEKLGAVIIGISKDSPKSHQKFVEKHSLSILLLSDEAHKVIEDYQAWGKKKMYGKEYFGVIRSTFLINPDGNIVKVWRNVKVKDHANEVLKTLKELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGH3</accession>
    <name>B7IGH3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">THA_717</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579750.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_717"/>
    <dbReference type="EnsemblBacteria" id="ACJ75187">
        <property type="protein sequence ID" value="ACJ75187"/>
        <property type="gene ID" value="THA_717"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_717"/>
    <dbReference type="eggNOG" id="COG1074">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001114_1_3_0"/>
    <dbReference type="OMA" id="YVGDRKQ"/>
    <dbReference type="OrthoDB" id="137860at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="10">
        <location>
            <begin position="1"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="11">
        <location>
            <begin position="399"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ75187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="1041" mass="123121" checksum="8F9AFD1A60DC2170" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGI4</accession>
    <name>B7IGI4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Electron transfer flavoprotein subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579758.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_731"/>
    <dbReference type="EnsemblBacteria" id="ACJ75198">
        <property type="protein sequence ID" value="ACJ75198"/>
        <property type="gene ID" value="THA_731"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_731"/>
    <dbReference type="eggNOG" id="COG2086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060196_2_1_0"/>
    <dbReference type="OMA" id="YNGGMVP"/>
    <dbReference type="OrthoDB" id="1027643at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="PTHR21294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ETF" evidence="1">
        <location>
            <begin position="22"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="260" mass="28949" checksum="65F4071FF6E29251" modified="2009-02-10" version="1">MNIVVCIKQVPDTTELKIDPITGTLIRKGVPSIMNYDDKAALEEALKLKDQIGAKVTAISMGPEQAKEVLEEALAMGADDAILISDKVFSGSDTWATSNVLSEAIKKIGFDVIFTGRQAIDGDTAQVGPQIAQKLGIPQVTYVRKLSYKEGKFLIEREMDDYIEIMEARPPVLFSVVKEANNPRYLDVRRLVDICQKDPIQIWNNNDLKIDESIIGLKGSPTKVKKTFTPTFEKETKVIEGSTDEKVQTLIKELKEKHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGL5</accession>
    <name>B7IGL5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydroxyacylglutathione hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579786.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_764"/>
    <dbReference type="EnsemblBacteria" id="ACJ75229">
        <property type="protein sequence ID" value="ACJ75229"/>
        <property type="gene ID" value="THA_764"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_764"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_5_2_0"/>
    <dbReference type="OMA" id="GAWGTNC"/>
    <dbReference type="OrthoDB" id="1581636at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="13"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="191" mass="21736" checksum="164C691CB781C681" modified="2009-02-10" version="1">MNYQVFKTSGALATNTYVFEDDGKIYIVDPGFGIGKFVSDKEVYVLLTHGHFDHIMGLSELNIKKVFISKEDREMLTNPSLNFSQLFGKSFSFNGDVEDIDEHFETIKAPGHTMGSRIIIIDDLIFTGDTVFCSTIGRVDLSGSREKMIETLKTLNERFSKMDKNLKILPGHNEQCTIKTLFKINPYFKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGU3</accession>
    <name>B7IGU3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Maltose transport system permease protein MalG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_846</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100476.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_846"/>
    <dbReference type="EnsemblBacteria" id="ACJ75307">
        <property type="protein sequence ID" value="ACJ75307"/>
        <property type="gene ID" value="THA_846"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_846"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_2_0"/>
    <dbReference type="OMA" id="IAFFIAW"/>
    <dbReference type="OrthoDB" id="1510342at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="71"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="279" mass="31323" checksum="31D4BF1F61448576" modified="2009-02-10" version="1">MQAKKIIYRFILYFLVAIILIWCIFPFYWAIISSLKPDKDLFEVYPSLFPKRITFENYIKVFTERPFHINILNSVIVAGVTTLASLLIGSLAAYAIARLRFKGKVIVMSLILAVSMFPQVSILGSLFVLLRKMHLINTYPGLILPYIAITLPLTTWILQNFFRELPKEVEESAAIDGCSRLRTLWSIVLPMAAPGLVATGLLTFISAWNEFLFALTFMQRPEKYTVPVAIALFKGASQYEIPWGQIMAAAVIVTTPLVILVLIFQKRIIAGLSAGSVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGV9</accession>
    <name>B7IGV9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_863</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579843.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_863"/>
    <dbReference type="EnsemblBacteria" id="ACJ75323">
        <property type="protein sequence ID" value="ACJ75323"/>
        <property type="gene ID" value="THA_863"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_863"/>
    <dbReference type="eggNOG" id="COG1044">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_9_0_0"/>
    <dbReference type="OMA" id="NDNYMGR"/>
    <dbReference type="OrthoDB" id="1465613at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="250" mass="27230" checksum="E5FAF4C732CA56CE" modified="2009-02-10" version="1">MYISETAKVGKNVKFGHNVVIEDNVVIGDEVVIGHNVVVKEGTVIGRGCVIADNTVLGKKPFKASASATTEEKTLPPLVLGEYVTIGANCVIYRGAVLKDFVFVGDLASIREDVEIGEYTIIGRGVTVENKTRIGKYVKIETEAYITAISTVEDYCFVAPEVTFTNDNFLGRTEERKKYFKGPTLRKGARIGANATILPGIEIGEDALIAAGAVVTKNVPPKKIFAGVPAKEFRDVPEEQLLKNQKYYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGW2</accession>
    <name>B7IGW2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Lipopolysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_866</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_866"/>
    <dbReference type="EnsemblBacteria" id="ACJ75326">
        <property type="protein sequence ID" value="ACJ75326"/>
        <property type="gene ID" value="THA_866"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_866"/>
    <dbReference type="eggNOG" id="COG2244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_7_5_0"/>
    <dbReference type="OMA" id="LFCTLVE"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="331"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="467" mass="53726" checksum="8F87299C27979ECA" modified="2009-02-10" version="1">MAMKNYIKSFFSFSIGTWIRAIIVFFSTPIISYLILPEEFGRASMFTLAYNIALLVTLLGLDQSLVRYYYEEEDKNNVFWSCLFPSVILGFFVSIFFIVFEKSLSIFLYGDYYKGIGYLFAFSLLSGIFQRFNQLSIRMQKRGFLFSFINVILALGNFGGTVVFALTVSRSFYAIIFGQIIGNIFALLVGFFVDKQSRSFSKIDFEKLLEFLKYGIPFIPSSILYWLFSSIDRISLRQFSTFTEIGLYSAAFKIVSIMQLIQVGFTTFWVPLAYEKYNSKAISDNFFEKANSLISAILFIFGLLLLAFKDLIIFLLFAKSYREASYIAPFLILYPIMYMLSETTVLGINFSKKTYWHIVITGVSAFVNFVGNNLLVPFLGAKGAAISTGLSYVVFFTLRTIIAQKYYTMHIKIKRIYMSVILIVIVASLGTFFKASYIYYIFCILVLLIYLLLYKDVISYVKDQMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGX3</accession>
    <name>B7IGX3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_877</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_877"/>
    <dbReference type="EnsemblBacteria" id="ACJ75337">
        <property type="protein sequence ID" value="ACJ75337"/>
        <property type="gene ID" value="THA_877"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_877"/>
    <dbReference type="eggNOG" id="COG0618">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1562138_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="171" mass="20315" checksum="FF476AEEFBACD859" modified="2009-02-10" version="1">MYDISLENNIKLSEEMLFSFAVALYTDTAMFRTARSTEFLYLSKFLSTKRFEEVLETIYFEKIGRKNFVNQIGNTEFYEINGLSIAVCKFNNQDEYYAFIDGLFDALSLDVFISIIPEGIKVHVKKRHVQKIYHRILVPLQKRLNVKRGHGIWFDFYNYNLMLDALREYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGX8</accession>
    <name>B7IGX8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integrase, catalytic region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_882</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_882"/>
    <dbReference type="EnsemblBacteria" id="ACJ75342">
        <property type="protein sequence ID" value="ACJ75342"/>
        <property type="gene ID" value="THA_882"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_882"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052819_0_0_0"/>
    <dbReference type="OMA" id="PRRTMYY"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025948">
        <property type="entry name" value="HTH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13276">
        <property type="entry name" value="HTH_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="100"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="273" mass="32776" checksum="AFED3238EAE7619F" modified="2009-02-10" version="1">MIEEGFSVTEAVRYLKISRSTYYYKPKKYKRKKNDESILKRIQEIKQEHPYWGYRRICAMLRKQGEKINHKRVYRIMKENRLLLKQYKMKAKRSFQRKIIPNKPRQVLGIDMTKVLTKDGGWVNYIAVIDWYTREVLGSQISLRGRTEEWLKALNNALNKGYKDGVRGREVILVSDNGTQPTSRRFMEECKILGIKQIFTSYNNPKGNANTERYFRTYKEEVVWPVEEMSYSELVARTKEYEEFYNSKYPHSALKYRSPREVYEEYLSFTKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH10</accession>
    <name>B7IH10_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS domain containing membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100794.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_915"/>
    <dbReference type="EnsemblBacteria" id="ACJ75374">
        <property type="protein sequence ID" value="ACJ75374"/>
        <property type="gene ID" value="THA_915"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_915"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_9_0_0"/>
    <dbReference type="OMA" id="WDKQVHQ"/>
    <dbReference type="OrthoDB" id="2014094at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="93"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="147" mass="16624" checksum="4FB907628628BE53" modified="2009-02-10" version="1">MKVKDFYIRDITAVLEDESVSRVLKILSRQQVTGVPVIDEDYKVVGFISENDIIRAALPSYFSLLQTASFIPDLNQFVRNLKKISNRAVSEIMTKPAITIKESTPLLHAADLMIRHSLKILPVVDEDDKLLGVITRMKILEAVSRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH13</accession>
    <name>B7IH13_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_918"/>
    <dbReference type="EnsemblBacteria" id="ACJ75377">
        <property type="protein sequence ID" value="ACJ75377"/>
        <property type="gene ID" value="THA_918"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_918"/>
    <dbReference type="HOGENOM" id="CLU_214861_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="47" mass="5286" checksum="3F90A3D5F648227F" modified="2009-02-10" version="1">MAKSVKILSQIELIKLTSSGNSEAKKELEKRLKDMVNSDEKHKKGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH16</accession>
    <name>B7IH16_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_921</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579879.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_921"/>
    <dbReference type="EnsemblBacteria" id="ACJ75380">
        <property type="protein sequence ID" value="ACJ75380"/>
        <property type="gene ID" value="THA_921"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_921"/>
    <dbReference type="eggNOG" id="ENOG50334Z2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_534124_0_0_0"/>
    <dbReference type="OMA" id="HYITVYA"/>
    <dbReference type="OrthoDB" id="451730at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="521" mass="58948" checksum="64E60316DEB0946C" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH23</accession>
    <name>B7IH23_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH-dependent butanol dehydrogenase a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_928</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100815.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_928"/>
    <dbReference type="EnsemblBacteria" id="ACJ75387">
        <property type="protein sequence ID" value="ACJ75387"/>
        <property type="gene ID" value="THA_928"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_928"/>
    <dbReference type="eggNOG" id="COG1979">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007207_0_4_0"/>
    <dbReference type="OMA" id="CTMALNG"/>
    <dbReference type="OrthoDB" id="717704at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990362">
        <property type="term" value="F:butanol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08187">
        <property type="entry name" value="BDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044731">
        <property type="entry name" value="BDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43633">
        <property type="entry name" value="PTHR43633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00913">
        <property type="entry name" value="ADH_IRON_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-ADH" evidence="3">
        <location>
            <begin position="9"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="383" mass="42005" checksum="298C37DDC91A3A01" modified="2009-02-10" version="1">MQNFVFYNPTKLVFGKNTVEKIGEYLKKDNVKKVLLLAGGGSIKKNGVYEKVVRSLVENGVEKIEVWGVRPNPVLSKVKEAISVAKNENVDAILAVGGGSVIDSAKAVAAGVRYEGDVWDFYSGKAKVKEAMPLYAILTLSATGTEMNGNSVVTNEETLEKFSFSSIHVYPKVSVVDPEVQYTLPQNQLVNGAVDAISHVMEYYFDGEEVSLTDRIDEGIIKTIMQTTEVLLDDPKNYDARANFALSTTLALNGVSGLGHKGGDWSSHAIEHALSALNPDVAHGAGLAVVFPAWMKYVYSQAPERFVQFGENIFGLKDCKPEEAINALSDWYKKIGAPTTLKELGFKEEDIEKLTEIASKMAPMGKLKKLERDDIKKILEIAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH32</accession>
    <name>B7IH32_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-N-acetylglucosamine 2-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_937</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-N-acetylglucosamine 2-epimerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579890.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_937"/>
    <dbReference type="EnsemblBacteria" id="ACJ75396">
        <property type="protein sequence ID" value="ACJ75396"/>
        <property type="gene ID" value="THA_937"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_937"/>
    <dbReference type="eggNOG" id="COG0381">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041674_1_0_0"/>
    <dbReference type="OMA" id="RYNTERP"/>
    <dbReference type="OrthoDB" id="1117971at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008761">
        <property type="term" value="F:UDP-N-acetylglucosamine 2-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003331">
        <property type="entry name" value="UDP_GlcNAc_Epimerase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029767">
        <property type="entry name" value="WecB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43174">
        <property type="entry name" value="PTHR43174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02350">
        <property type="entry name" value="Epimerase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00236">
        <property type="entry name" value="wecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase_2" evidence="2">
        <location>
            <begin position="25"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="354" mass="40110" checksum="A62C0015BF585776" modified="2009-02-10" version="1">MKLAIVCGTRPEVIKVAPVYLKAKEMGIDVKLIATAQHRQMMDVMLNVFSIKPDYDLDVMRENQTLNLISSRIFSRIDEIFEREKFEWLFVQGDTTTAMTSAIAAFNRGIKVGHIEAGLRSNNLKDPFPEEMNRRVIDQVSDLMFAPTKKAYENLKKEGYEDERIKITGNTVVDALYYVKNRFELEKIRKSLVDVKEYVLVTLHRRENWGEKMKKILRGIKRFSVEKDIPIVFPVHLNPKVRKVVNQELGDFKNAKLLDPVGYVEFLSLLSGAKIVASDSGGIQEEAPSFGKFVVVCRNTTERPELIESGYGVLAGTEEESVYKSLIEGLDRKLAVDNPFGDGKASEKILSAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH34</accession>
    <name>B7IH34_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100845.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_939"/>
    <dbReference type="EnsemblBacteria" id="ACJ75398">
        <property type="protein sequence ID" value="ACJ75398"/>
        <property type="gene ID" value="THA_939"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_939"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_15_1_0"/>
    <dbReference type="OMA" id="KPWCAGI"/>
    <dbReference type="OrthoDB" id="1746871at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857413" evidence="1">
        <location>
            <begin position="20"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="415" mass="46328" checksum="367706E016AC5ED8" modified="2009-02-10" version="1">MKKILIIAFAIILSLSIFATENALEIFSWWTGGGEEEGLLALFDLFNKYYPDIQIINAAVAGGAGTNAKAVLKTRMLGGNPPDSFQVHAGMELTDTYVIPGLMEPITWLLKETGAYDKFPKDLIEIVSYKGEVYSVPVNVHRANVVFYNKKIAKEIGMTKEPTTWPEFLTYLDKAKKMGYVGIALGDKNKWTSLHLFESIMLAELGPEKYNKLWKGEASFNDPAIKQALSILKDILAYVNSDHSALTWQDATRMVFEGKAFCNMMGDWAEGYLKTLGWKPGVDFGWFALPGTQDAFMLVSDTFGLPKNAPHRENAIKWLKFLATVEAQDTFNPIKGSIPARIDADKSKYDVYLKWSMNDFSTKAICPSIIHGSAAPESFATALMDAINIFVTEQNVNQTFKEILWIAEDNGYLTD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH57</accession>
    <name>NUSB_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription antitermination protein NusB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Antitermination factor NusB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nusB</name>
        <name type="ordered locus">THA_962</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NusB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579905.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IH57"/>
    <dbReference type="SMR" id="B7IH57"/>
    <dbReference type="STRING" id="484019.THA_962"/>
    <dbReference type="EnsemblBacteria" id="ACJ75421">
        <property type="protein sequence ID" value="ACJ75421"/>
        <property type="gene ID" value="THA_962"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_962"/>
    <dbReference type="eggNOG" id="COG0781">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087843_3_3_0"/>
    <dbReference type="OMA" id="TKQFCNL"/>
    <dbReference type="OrthoDB" id="1574817at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00073">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011605">
        <property type="entry name" value="NusB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11078">
        <property type="entry name" value="PTHR11078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01951">
        <property type="entry name" value="nusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0889">Transcription antitermination</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_1000117057" description="Transcription antitermination protein NusB">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00073"/>
        </source>
    </evidence>
    <sequence length="142" mass="16708" checksum="ADC3C65C7BA335C1" modified="2009-02-10" version="1">MIKRRHKMRESVFKALFQWDFQKDEDLTEIAKQHVSLLEDEKLREQAYSYIEGIKKTIESLDEIISKYLQNWTLDRLSVTDRNILRLGTYELLYVDDVPIEVTLDEMIELGKTYGTENSGKFVNGVLDRIAKGEAPKEKFDM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH68</accession>
    <name>SURE_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5'-nucleotidase SurE</fullName>
            <ecNumber evidence="1">3.1.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside 5'-monophosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">surE</name>
        <name type="ordered locus">THA_973</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100941.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IH68"/>
    <dbReference type="SMR" id="B7IH68"/>
    <dbReference type="STRING" id="484019.THA_973"/>
    <dbReference type="EnsemblBacteria" id="ACJ75432">
        <property type="protein sequence ID" value="ACJ75432"/>
        <property type="gene ID" value="THA_973"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_973"/>
    <dbReference type="eggNOG" id="COG0496">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045192_1_3_0"/>
    <dbReference type="OMA" id="DCVHIAL"/>
    <dbReference type="OrthoDB" id="1909278at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106411">
        <property type="term" value="F:XMP 5'-nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00060">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="PTHR30457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SSF64167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000196616" description="5'-nucleotidase SurE">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00060"/>
        </source>
    </evidence>
    <sequence length="255" mass="28519" checksum="BF2AC956690A35DD" modified="2009-02-10" version="1">MNILVTNDDGVTADGILCLARTLSKKYKVTVVAPETEQSAVGHAITLRLPLWLRKLDINENFEIYSVSGTPADCVKMGIDVVLGEKPDLLISGINRGNNLGTDVVYSGTVSGALEGAIAGVPSIAVSSYSFENPMYETAAKFILDFLEEFDVRSIPRFTALNINVPSVPYDQIKGWKLTRQSKRMYDDYFEKRVDPSGGNYYWMMGTIIEDDPDPKADYKAIAENYVSVTPISVFLTNEEYLKRLEERYEDKTIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH87</accession>
    <name>B7IH87_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Histidinol-phosphatase</fullName>
            <shortName evidence="7">HolPase</shortName>
            <ecNumber evidence="4 7">3.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_992</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H2O + L-histidinol phosphate = L-histidinol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14465"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="3.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PHP hydrolase family. HisK subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.15" evidence="4 7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100984.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_992"/>
    <dbReference type="EnsemblBacteria" id="ACJ75451">
        <property type="protein sequence ID" value="ACJ75451"/>
        <property type="gene ID" value="THA_992"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_992"/>
    <dbReference type="eggNOG" id="COG1387">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054611_3_0_0"/>
    <dbReference type="OMA" id="THHSYDS"/>
    <dbReference type="OrthoDB" id="1615112at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00013"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004401">
        <property type="term" value="F:histidinol-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010140">
        <property type="entry name" value="Histidinol_P_phosphatase_HisJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21039">
        <property type="entry name" value="PTHR21039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01856">
        <property type="entry name" value="hisJ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POLIIIAc" evidence="8">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="238" mass="28000" checksum="81F8714EA6781035" modified="2009-02-10" version="1">MIDYHIHSNFSPDSNSSVEDIVKYAREKNLKIIITDHYEAVEKDHNFKFDVKSYKEAMKKYGLLVGVEFGWDGKSKIDIDLKEFDFVILSYHAYKEYDDIQKMYENYLKDLYNIIEVVNDFHVLGHLDFPRRFVKNNEPFSKKLYPLISEIFKKIIKEGKILEVNTSAIYRYGEPNPSYDILKLYRDLGGKYVTIGSDAHRLEDVGRGIYDTLGNLKDLGFEYIMVLNDGKWDMEKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHB7</accession>
    <name>B7IHB7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1023"/>
    <dbReference type="EnsemblBacteria" id="ACJ75481">
        <property type="protein sequence ID" value="ACJ75481"/>
        <property type="gene ID" value="THA_1023"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1023"/>
    <dbReference type="HOGENOM" id="CLU_3223194_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="44" mass="5444" checksum="DCA3F790D4BD82E2" modified="2009-02-10" version="1">MLKQMIDFSKKLKRENFYFQDEELGEDIVYVIISLEDKKSFYYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHC2</accession>
    <name>B7IHC2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sensory box protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579943.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75486">
        <property type="protein sequence ID" value="ACJ75486"/>
        <property type="gene ID" value="THA_1028"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1028"/>
    <dbReference type="eggNOG" id="COG2461">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026706_1_0_0"/>
    <dbReference type="OMA" id="LCHPPKS"/>
    <dbReference type="OrthoDB" id="565213at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007380">
        <property type="entry name" value="DUF438"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04282">
        <property type="entry name" value="DUF438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF438" evidence="2">
        <location>
            <begin position="11"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Hemerythrin" evidence="1">
        <location>
            <begin position="86"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="418" mass="49730" checksum="C7AE0AE68260BA64" modified="2009-02-10" version="1">MSEVFNKREYLKNLIKRTKTEDSEKLKKEIQQILSEISPLEIAIVEQELMNEEGISVDEVRKVCNIHIDVLESKTKGPTLDVPKWHPIYILVKEHEHFQEKVVEFKKLVDQINQKDNFSEAMPLIIKIQEFIDEFENLELYFQKEENVLFPVIEKHGIEKPPMVMWKEHDVFRTLRKELKQLKENRQINFEDLKEKLWNISTAMVDLILNHIYKEHFVLFPSSLETITDEEWKTIREEFDKIGYCCYKPEEYSKEDDNINDEDITIDKINSMIKKYLEKSSSDKIEFPSGVLTKKQLLWILNTLPVDITFVDENDEVKYFSETKDRIFVRSRTIIGRKVQNCHPEKSIEIVNKILSDFKSGKRDKADFWLKLGEKYVYIQYFAVRDENGKYLGTLEVTQDIAPIQKITGEKRIYDDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHC4</accession>
    <name>B7IHC4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1030"/>
    <dbReference type="EnsemblBacteria" id="ACJ75488">
        <property type="protein sequence ID" value="ACJ75488"/>
        <property type="gene ID" value="THA_1030"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1030"/>
    <dbReference type="HOGENOM" id="CLU_3223195_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="44" mass="4951" checksum="CCC10F756F23F43E" modified="2009-02-10" version="1">MLFFETKNENSKTSGLVKQNSSISGMKKSPKSLISFNPPYVKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHC8</accession>
    <name>B7IHC8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Secretin and TonB N terminus short domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579947.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1034"/>
    <dbReference type="EnsemblBacteria" id="ACJ75492">
        <property type="protein sequence ID" value="ACJ75492"/>
        <property type="gene ID" value="THA_1034"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1034"/>
    <dbReference type="eggNOG" id="COG4796">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059922_0_0_0"/>
    <dbReference type="OMA" id="IFFATRI"/>
    <dbReference type="OrthoDB" id="870677at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="360" mass="40939" checksum="CBC6CE0754B5F699" modified="2009-02-10" version="1">MKKITFLFLLIWGIVFSLTVSFYEQDIKEALNILSLESGKKIVYEPNLSGIVSIEIDEDLSLEEILDIILLPYNYYWVKYDEIYFVGVSNPESPGFFKNAKIFNIPLRYKTASELSKVLPKELKEYILYSSSEDSITVFAPIPVASQIANIVSILDVKKKSYTANIKIIDVSESYLKSFLLKINYSQERLNDLLSGAIFSIPLYGNYLNLLFSSTTENKDFDLIYDGNIKMLSGVKSSLRTSKKVLVDEIVDNKISKKAVESNISFELTPYFLKSSALIDLNINLENIGTDRLGSQVSTKVPIDFSVPKVIGAYSYLKSERADGGLSFLMDLPIIGNLFKQNFEKHEKRYILFVLRIEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHD3</accession>
    <name>B7IHD3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cob(I)alamin adenosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cobO</name>
        <name evidence="1" type="ordered locus">THA_1039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101074.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1039"/>
    <dbReference type="EnsemblBacteria" id="ACJ75497">
        <property type="protein sequence ID" value="ACJ75497"/>
        <property type="gene ID" value="THA_1039"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1039"/>
    <dbReference type="eggNOG" id="COG2109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088595_2_0_0"/>
    <dbReference type="OMA" id="HAMGEGF"/>
    <dbReference type="OrthoDB" id="1690483at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:cob(I)yrinic acid a,c-diamide adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00561">
        <property type="entry name" value="CobA_CobO_BtuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003724">
        <property type="entry name" value="CblAdoTrfase_CobA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02572">
        <property type="entry name" value="CobA_CobO_BtuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015617">
        <property type="entry name" value="Adensltrnsf_CobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00708">
        <property type="entry name" value="cobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="169" mass="19413" checksum="E4C44443F4CDC8E4" modified="2009-02-10" version="1">MGYIHVYTGNGKGKTTAAFGLALRAACADKKVFIGQFIKGMDYSELRVPEFIKNIEIEQFGRNCFIFNKPTQEDIDAAKKGLKRIEEVLKSKKYDVVILDEVNVAIYYKLFSVEDVLEVLNNRDEKVEIILTGRYAPEKFIEIADLVTEMKEIKHYYKKGVKARKGIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHG9</accession>
    <name>B7IHG9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydroxymethylbilane synthase</fullName>
            <ecNumber evidence="4">2.5.1.61</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hemC</name>
        <name evidence="8" type="ordered locus">THA_1077</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579983.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1077"/>
    <dbReference type="EnsemblBacteria" id="ACJ75533">
        <property type="protein sequence ID" value="ACJ75533"/>
        <property type="gene ID" value="THA_1077"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1077"/>
    <dbReference type="eggNOG" id="COG0181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019704_1_0_0"/>
    <dbReference type="OMA" id="NAHEWAG"/>
    <dbReference type="OrthoDB" id="1450400at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PTHR11557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobil_deam" evidence="7">
        <location>
            <begin position="3"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="280" mass="31500" checksum="83318141AF610C4F" modified="2009-02-10" version="1">MKIKVGTRGSKLALIQTNMVVERLKSKLSNVSFEVIPIKTKGDIVKKPVGEIGGKGVFVSDIEKLLLNGEIDIAIHSMKDMPSKIPDGLYLTSVLKREDARDVFVGKKDFFLLESGAKIGTSSKRRMLQLSILRPDIEIVPIRGNVDTRLKRIGELDGIVLAAAGLKRLGLEKKITNYFSIEQVVPAPCQGILALEFTSKFLPLFEKVKDEIVDPDTEFVSKIERKVLSISGFDCNTPFGFYCEKNDDNLVFHIFKNEKRERLVADKERAYEVLGEIRWE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHK5</accession>
    <name>SYH_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Histidyl-tRNA synthetase</fullName>
            <shortName evidence="1">HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisS</name>
        <name type="ordered locus">THA_1116</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-histidyl-tRNA(His)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580009.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHK5"/>
    <dbReference type="SMR" id="B7IHK5"/>
    <dbReference type="STRING" id="484019.THA_1116"/>
    <dbReference type="EnsemblBacteria" id="ACJ75569">
        <property type="protein sequence ID" value="ACJ75569"/>
        <property type="gene ID" value="THA_1116"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1116"/>
    <dbReference type="eggNOG" id="COG0124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_1_1_0"/>
    <dbReference type="OMA" id="CDFDFIG"/>
    <dbReference type="OrthoDB" id="277998at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000199161" description="Histidine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00127"/>
        </source>
    </evidence>
    <sequence length="418" mass="47932" checksum="97F0F101018D9E02" modified="2009-02-10" version="1">MYKRIKGTEDIFGDDIKYWYYIENVVKETVRLYGYSEIRTPIFEATELFIRSVGEETDIVQKEMYTFEDKGQRSITLRPEGTAPTIRAFIENSMMATGLPKRLFYIGPMFRYERPQKGRQRQFHQFGVELIGSPSPLADAEAIIVADRVLKNLGLLNYTIKINSIGCDKCRANYKNALKEYYSDKLDYVCDDCKRRYNTNVLRLLDCKVDVEYIKDAPKITDYLCDDCKHHYEETKRLLDNLKIKYVEDPLLVRGLDYYNGVVFEIHHGDLGAQSAVGGGGRYDKLIKELGGSQTPSLGFAMGIERLIIALKNEKIPVEEIKNNEIFVAHLGEQARIEALKIAEDLRDNGISVVFSTMERGLSAQLKHAARLKCKLCVIVGENELERNIVILRNMETGEQIEIERDYVVGTAKEWIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHM6</accession>
    <name>B7IHM6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0473 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580026.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1138"/>
    <dbReference type="EnsemblBacteria" id="ACJ75590">
        <property type="protein sequence ID" value="ACJ75590"/>
        <property type="gene ID" value="THA_1138"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1138"/>
    <dbReference type="eggNOG" id="ENOG5033145">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146610_1_0_0"/>
    <dbReference type="OMA" id="YWVCEEI"/>
    <dbReference type="OrthoDB" id="2057244at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009711">
        <property type="entry name" value="UPF0473"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06949">
        <property type="entry name" value="DUF1292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="109" mass="13090" checksum="AF006EF72088162B" modified="2009-02-10" version="1">MHDHHHEHEHEHLEAFTLYDEEGNEHNFILLAEIENEGSDYWACEEIFVENEEITDFGELYIFKKTVDEEGNVFLDTIEDEDEFNRVAKMFEEMSDFEIVDDENEDIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHN4</accession>
    <name>B7IHN4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580034.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1146"/>
    <dbReference type="EnsemblBacteria" id="ACJ75598">
        <property type="protein sequence ID" value="ACJ75598"/>
        <property type="gene ID" value="THA_1146"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1146"/>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_668902_0_0_0"/>
    <dbReference type="OMA" id="YKCVAVY"/>
    <dbReference type="OrthoDB" id="400128at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="411" mass="48056" checksum="0B9E7249895731C6" modified="2009-02-10" version="1">MRHLFYLQQFFERLPSPAFIKDNKFRYVWINKEWKKTYELDDRKVIGKTDQKIFGNTISEKIETKVIKSKKNQEYEKEYKDKFFRVVVIPIRLGDGTYGVAGIEFDETKRYFNDLVLQANLKVSEIIRELLVSPLESKDFFVERLLDKVQEKMHLEKYALIKNNEIIKSFFPENVCLKAMTKEGIKEFSVNTVKYIVIPFDEYKVVINSSPITLKLTNYIVPLILPKVENVLSKIENEARKKQYYTTMEKMVEAVISWKKEKLEDFLQKVLEDIVQIIPEAEKGTVWLIKDGKYKCVAEVGYKDATKLEFPPEKTSYGQKIEEMIKEGYTVFEIEGAKDLVSTSPLAETFKNYGMFDPNFRPLLGVFKVGNNVIGNISIDNFSGISFSSESKKILEQYVKILSNFLEEYEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHU7</accession>
    <name>RL4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplD</name>
        <name type="ordered locus">THA_1216</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101441.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHU7"/>
    <dbReference type="SMR" id="B7IHU7"/>
    <dbReference type="STRING" id="484019.THA_1216"/>
    <dbReference type="PRIDE" id="B7IHU7"/>
    <dbReference type="EnsemblBacteria" id="ACJ75661">
        <property type="protein sequence ID" value="ACJ75661"/>
        <property type="gene ID" value="THA_1216"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1216"/>
    <dbReference type="eggNOG" id="COG0088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041575_5_2_0"/>
    <dbReference type="OMA" id="PQVHILE"/>
    <dbReference type="OrthoDB" id="1572673at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_B">
        <property type="entry name" value="Ribosomal_L4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_L4/L1e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_L4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4/L1e"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="PTHR10746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="SSF52166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03953">
        <property type="entry name" value="rplD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000142196" description="50S ribosomal protein L4">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="221" mass="24796" checksum="D2E87D3D54C42CBC" modified="2009-02-10" version="1">MAKVSLFNSKGENIGPIDLKDEVFAIEPNFDVIWRYVDMQLTNARAGTASTKTRGEVSGGGRKPWIQKHTGRARQGSIRAPHWRHGGVAHGPKPRVYFKRLNKKMKRLALKSALSLRLKEGNLIVVDEIKFKRPKTKDLREVLKNLGLENQKVLVVLPKKVEEYVNVKISGRNIPGVKVIIADNPGVDRTNIDGLNVYDILNHDKLVLLQGTVQKIEEVLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHV6</accession>
    <name>RL14_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplN</name>
        <name type="ordered locus">THA_1225</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L19. In the 70S ribosome, L14 and L19 interact and together make contacts with the 16S rRNA in bridges B5 and B8.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101458.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHV6"/>
    <dbReference type="SMR" id="B7IHV6"/>
    <dbReference type="STRING" id="484019.THA_1225"/>
    <dbReference type="EnsemblBacteria" id="ACJ75670">
        <property type="protein sequence ID" value="ACJ75670"/>
        <property type="gene ID" value="THA_1225"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1225"/>
    <dbReference type="eggNOG" id="COG0093">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095071_2_1_0"/>
    <dbReference type="OMA" id="AKEVLCI"/>
    <dbReference type="OrthoDB" id="1799923at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_L14_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_L14P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005745">
        <property type="entry name" value="Ribosomal_L14P_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_L14P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="PTHR11761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="SSF50193"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01067">
        <property type="entry name" value="rplN_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000144341" description="50S ribosomal protein L14">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <sequence length="122" mass="13520" checksum="C77741B834062B73" modified="2009-02-10" version="1">MIINESYLNVADNSGAKLLRVIRVMGGSRRKWGTVGDVVVCSVRDAVPNGDLKKGDVVKAVIVRTKKEIRRPDGTYIRFDDNAAVVLDKYNEPKGTRVFGPVAKELREKGFMKIVSLAPEVF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHW3</accession>
    <name>RS5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsE</name>
        <name type="ordered locus">THA_1232</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S4 and S8.</text>
    </comment>
    <comment type="domain">
        <text>The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101468.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHW3"/>
    <dbReference type="SMR" id="B7IHW3"/>
    <dbReference type="STRING" id="484019.THA_1232"/>
    <dbReference type="EnsemblBacteria" id="ACJ75677">
        <property type="protein sequence ID" value="ACJ75677"/>
        <property type="gene ID" value="THA_1232"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1232"/>
    <dbReference type="eggNOG" id="COG0098">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065898_2_2_0"/>
    <dbReference type="OMA" id="KRGCGSW"/>
    <dbReference type="OrthoDB" id="1505038at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_B">
        <property type="entry name" value="Ribosomal_S5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_S5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005712">
        <property type="entry name" value="Ribosomal_S5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_S5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_S5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_S5_N_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="PTHR13718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01021">
        <property type="entry name" value="rpsE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000140898" description="30S ribosomal protein S5">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="S5 DRBM" evidence="1">
        <location>
            <begin position="15"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <sequence length="176" mass="18908" checksum="8FB2D79A33A112C0" modified="2009-02-10" version="1">MADIAQKIRNTREDFEERIVEIRRTTKVTKGGKNLSFRVLAVVGNRNGKVGVGVGKAREVPDAIRKALSAARRNVFEVPIYNGTIPHEIVGRQDAAKVLLKPAAPGTGIISNGTVRAVVELAGIHNILTKTSGSTNPVVLAQATVNGLKNLLSLEKIAQLRDITPQEVIYGVKKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHX3</accession>
    <name>B7IHX3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">DNA-directed RNA polymerase subunit alpha</fullName>
            <shortName evidence="11">RNAP subunit alpha</shortName>
            <ecNumber evidence="2 11">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">RNA polymerase subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">Transcriptase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">rpoA</name>
        <name evidence="13" type="ordered locus">THA_1242</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="domain">
        <text evidence="11">The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the RNA polymerase alpha chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2 11"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101496.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1242"/>
    <dbReference type="EnsemblBacteria" id="ACJ75687">
        <property type="protein sequence ID" value="ACJ75687"/>
        <property type="gene ID" value="THA_1242"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1242"/>
    <dbReference type="eggNOG" id="COG0202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053084_0_1_0"/>
    <dbReference type="OMA" id="LMKFRNF"/>
    <dbReference type="OrthoDB" id="662686at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00059">
        <property type="entry name" value="RNApol_bact_RpoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011773">
        <property type="entry name" value="DNA-dir_RpoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011260">
        <property type="entry name" value="RNAP_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32108">
        <property type="entry name" value="PTHR32108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03118">
        <property type="entry name" value="RNA_pol_A_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="SSF55257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="SSF56553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02027">
        <property type="entry name" value="rpoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RPOLD" evidence="12">
        <location>
            <begin position="22"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Alpha N-terminal domain (alpha-NTD)" evidence="11">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Alpha C-terminal domain (alpha-CTD)" evidence="11">
        <location>
            <begin position="260"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ75687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="330" mass="37546" checksum="65BD7C06D0863A7B" modified="2009-02-10" version="1">MEFVMPKRLKMEEHKESEDYYYARYVLSPLEKGYATTIGNTLRRVLLSSIPSLAITDVKFVRPEKYHEFDTIEGVKEDILEILLNLKKVQLSMEDYVEDPVELTIQHKGAGEIKAGDIIVPAGVSVTNPNLHIATLNEDADLEIKLYATIGKGFVPAAERNEKPDIGWIVLDGVYNPVLKVNWIAENVRVGKKTDYDKLVLEIWTKKNIKPSDALKKAVKIINDHFRIIEESFGEFDEENDIAEVSEVSTVEEEEVVDENDILSRKIDELDLSMRALNCLKRDKIETIGDLLSRGEEELLKIKNFGQKSLDEVKEKLMEKFGLSFGKGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICQ5</accession>
    <name>B7ICQ5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1337</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101688.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1337"/>
    <dbReference type="EnsemblBacteria" id="ACJ75782">
        <property type="protein sequence ID" value="ACJ75782"/>
        <property type="gene ID" value="THA_1337"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1337"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_92_10_0"/>
    <dbReference type="OMA" id="WINLLYM"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="5"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="HD-GYP" evidence="3">
        <location>
            <begin position="255"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="447" mass="52041" checksum="8CEB5C33AF09F490" modified="2009-02-10" version="1">MDKWKILIVDDEPDVHTITSIILKDIEFKGKKIKMINAYSLEQAIEILKNEKDIALAIVDIVMENKNDGFKLIQFIRETLKNRLMRIVVRTGQPGVAPPREIILKYDINDYREKSEFSSSALFTLVISKLREYSDLLELYNQREILEEFANINFHNLDDLNLFFEKIKNILSTNINIEYSVLDYSENEDKCDIIDKTLWKSNNEVEICIKAEKEKRLKYTIKFDNQLSNAYKNILNLTLSKQILNIENNILSKELIDNLYQIIYILSETTETRSFETGEHVKRVGLITKVIAKELGFKNQKLEFISTAAMLHDIGKIGIPDAILNKPTKLEDNEWEVMKQHTIIGYKILSTVDNPLFKMASNIALYHHENWDGSGYPKGLKGEEIPLEARIVALVDVYDALSTDRVYRKAWSEEKVLKYIKELNGKKFDPNIVEIFLNNYDEIKKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICS4</accession>
    <name>B7ICS4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Base excision repair protein, HhH-GPD family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1356"/>
    <dbReference type="EnsemblBacteria" id="ACJ75801">
        <property type="protein sequence ID" value="ACJ75801"/>
        <property type="gene ID" value="THA_1356"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1356"/>
    <dbReference type="eggNOG" id="COG2231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_6_0_0"/>
    <dbReference type="OMA" id="GPQGWWP"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="1">
        <location>
            <begin position="32"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="210" mass="24552" checksum="86A919E7FD6C50F1" modified="2009-02-10" version="1">MKNLYKLLLKAYGSVGKWWPGTSEEIIVTAVLTQNTNWKNVERALENIYKNKTKENLLEYLYELPIGYLSELIKPAGFFNLKARRLKNLLSFLKEYDFELSKIKRLKNLREKLLKINGIGKETADSILLYALEIPVFVVDAYTKRLLKRMYNINLSDYDSVQNLFYENYPKNVKLFQELHGLIVEHSKAVCRKNPICSECKISDNCKKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICS8</accession>
    <name>B7ICS8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1360"/>
    <dbReference type="EnsemblBacteria" id="ACJ75805">
        <property type="protein sequence ID" value="ACJ75805"/>
        <property type="gene ID" value="THA_1360"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1360"/>
    <dbReference type="eggNOG" id="ENOG50342UC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1618209_0_0_0"/>
    <dbReference type="OrthoDB" id="1170010at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="164" mass="17751" checksum="2A8126E3CD9096B9" modified="2009-02-10" version="1">MLFLIIFQTLFPANIAKIPLNDILKDVSLLCLGSLGDLTLSYDAGLAAGYLLKQQGYDAYVVGTLDTLSKDDKDPLNRVNVSAFITAHVYSLFARGLATAGIIPIFDGTILDKEVVVSLNTRDATYPIVVDSEVKKNLLSELGYNGSVFLSNEIGKYIDGIKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICT8</accession>
    <name>B7ICT8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type multidrug transport system, ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1370</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580186.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1370"/>
    <dbReference type="EnsemblBacteria" id="ACJ75815">
        <property type="protein sequence ID" value="ACJ75815"/>
        <property type="gene ID" value="THA_1370"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1370"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_0"/>
    <dbReference type="OMA" id="AMTREYM"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="255" mass="29207" checksum="CC08BCAE5800FA49" modified="2009-02-10" version="1">MIELFSVSKRYKKDWVVKDISFSALDGEVIGIIGRNGCGKSTILKMVAGIIRPTRGKIKLNSNLISYIPEKPVLIPELTLEENIGYFASIRNVSKEKIDKEIEYFQLSSHLKKRPMELSKGLQQRLSMAVALLIDPDIVLLDEPTSGLDAESKKLILNRIKELKMNKKTILYVTHDDEEIEKICDKVLILNKGVKVFFGSVEDFWMKYERFVYVTLAKDKETKLVRIEDLKNFEDIVHIRSVGIREYLGGLENGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICX4</accession>
    <name>B7ICX4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2520 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580209.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1407"/>
    <dbReference type="EnsemblBacteria" id="ACJ75851">
        <property type="protein sequence ID" value="ACJ75851"/>
        <property type="gene ID" value="THA_1407"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1407"/>
    <dbReference type="eggNOG" id="COG5495">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055635_1_0_0"/>
    <dbReference type="OMA" id="WQVVEEE"/>
    <dbReference type="OrthoDB" id="1194694at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018931">
        <property type="entry name" value="DUF2520"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037108">
        <property type="entry name" value="TM1727-like_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10728">
        <property type="entry name" value="DUF2520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2520" evidence="1">
        <location>
            <begin position="125"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="262" mass="29370" checksum="217780B2BC296FBF" modified="2009-02-10" version="1">MKINIVGIGKVTTAILFNLKDKVDIGYIVSRDLNKAKKLSDELGKGIPVTYNDNFKFEDIVLVGLNDSTLPDSVDLIKNFVSEGNTVIHFSGFHPSTIFPKEWNPASMHPNCPVLGKETSFKDIVFGIEGNTKITKEIVNLLGGKYFEIPSESKVLYHLSAVLMSNFPLALVYLAEKFYKNANLPHEMFLEVMESLLKTTIENVKKNGTLNSITGPIYREDVDIVNVEMEIFCSTFPELCNLFDGFIQIILSMKEEKESESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID26</accession>
    <name>B7ID26_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="13">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">pgsA</name>
        <name evidence="13" type="ordered locus">THA_1460</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="10">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101927.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1460"/>
    <dbReference type="EnsemblBacteria" id="ACJ75903">
        <property type="protein sequence ID" value="ACJ75903"/>
        <property type="gene ID" value="THA_1460"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1460"/>
    <dbReference type="eggNOG" id="COG0558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051314_2_2_0"/>
    <dbReference type="OMA" id="WSMVYYL"/>
    <dbReference type="OrthoDB" id="1702107at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004570">
        <property type="entry name" value="Phosphatidylglycerol_P_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000847">
        <property type="entry name" value="Phos_ph_gly_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00560">
        <property type="entry name" value="pgsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ75903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="174" mass="19598" checksum="7240CA82E6E97D25" modified="2009-02-10" version="1">MNVPNFITWVRVVLTAIIVFLLLNGFYLSAFVLFLIASISDYFDGYFARKLNQVTNFGKIFDQMSDKILITSILIVFVQLGLVPSWILVVIVFRDTLVSTVRMAAAYGNKVIAANYFGKLKTVSQMVWTIGIFLQLVGFESLVIFNVLLSYLVVFLTVVSGLVYMVQNKEILKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID39</accession>
    <name>B7ID39_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit PurS</fullName>
            <shortName evidence="5">FGAM synthase</shortName>
            <ecNumber evidence="5">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Formylglycinamide ribonucleotide amidotransferase subunit III</fullName>
            <shortName evidence="5">FGAR amidotransferase III</shortName>
            <shortName evidence="5">FGAR-AT III</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">purS</name>
        <name evidence="6" type="ordered locus">THA_1473</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PurS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580249.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1473"/>
    <dbReference type="EnsemblBacteria" id="ACJ75916">
        <property type="protein sequence ID" value="ACJ75916"/>
        <property type="gene ID" value="THA_1473"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1473"/>
    <dbReference type="eggNOG" id="COG1828">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164833_2_0_0"/>
    <dbReference type="OMA" id="DIQYKSN"/>
    <dbReference type="OrthoDB" id="2012814at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01926">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003850">
        <property type="entry name" value="PurS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696">
        <property type="entry name" value="PTHR34696"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02700">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="SSF82697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00302">
        <property type="entry name" value="TIGR00302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="82" mass="9441" checksum="DCB7D0D626EB4368" modified="2009-02-10" version="1">MQKFKFVVDIQYKSNVRDPRGETIARVLREESNIPIESIRIGKSIHGEVQAESKEEAEKLVIKACEELLVNPVTEEFKVEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID42</accession>
    <name>B7ID42_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cysteine synthase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cysK</name>
        <name evidence="4" type="ordered locus">THA_1476</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580252.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1476"/>
    <dbReference type="EnsemblBacteria" id="ACJ75919">
        <property type="protein sequence ID" value="ACJ75919"/>
        <property type="gene ID" value="THA_1476"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1476"/>
    <dbReference type="eggNOG" id="COG0031">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_0"/>
    <dbReference type="OMA" id="RTPVFKF"/>
    <dbReference type="OrthoDB" id="1033353at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PALP" evidence="3">
        <location>
            <begin position="2"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="285" mass="31001" checksum="6C12AE363D743770" modified="2009-02-10" version="1">MIGNTPVYFIEELGIYAKFERNNPSGSVKDRAAYFMIKAAINNNLIKNGIIVEPTSGNTGISLSWIGKKLGLKTILTMPESMSKERIEIMKAFGADVVLTPADKGMKGAIEKAKEIVAEKDAFMPNQFENPFNVLAHELTTGPEILRQTNFELDVFVAGVGTGGTISGVGRVLKKFFNKKVKIVAVEPENSAVLSGKNPGKHKIQGIGAGFIPKILDQTVIDKIETVKDEEVLDMFVYLNKKLGLNVGISAAANALVATKYKNFGKVLTIFPDDVTKYMSILDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID51</accession>
    <name>B7ID51_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transporter, major facilitator family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1487</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580255.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1487"/>
    <dbReference type="EnsemblBacteria" id="ACJ75928">
        <property type="protein sequence ID" value="ACJ75928"/>
        <property type="gene ID" value="THA_1487"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1487"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025894_0_0_0"/>
    <dbReference type="OMA" id="RISPQVW"/>
    <dbReference type="OrthoDB" id="626204at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="286"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="358"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="3"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="383" mass="42746" checksum="030281877CCB281C" modified="2009-02-10" version="1">MSAPVRIVFYTFLNGISRNVYQVLFNLYLKNFGYDNSIIGKIMSFNLWGSAILAVFLGILSDKLGRKKMLLFVQPLGALFAIMRLFPVNLTWLYATSFLFGGFNSAIMILTDVFLVESTHNKNRAKFFGMNFGVRMLTGVFGNALGGFLGDVFGFRNVMIWSMVLRIFALIPILKINEVVVLKEKIKMDEFQKKIFAFYIFSTASVGFGAGLFIHFGNVIFYDLFALSATVIGFILALAQFGTSIGSTFSHKLGKKFGASKVLMLSNLAVPILILMLSFVREPISFTAIYVARFTIMNMVNPIFNTLVLSYLPKSILASTAGIRSFANNTSRAFAAMLFSVLATSSSGYSIIFLISSIFYFVNALVIYVFYRIMKGKDSEFYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID53</accession>
    <name>B7ID53_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methylated-DNA--protein-cysteine methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1489</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101975.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1489"/>
    <dbReference type="EnsemblBacteria" id="ACJ75930">
        <property type="protein sequence ID" value="ACJ75930"/>
        <property type="gene ID" value="THA_1489"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1489"/>
    <dbReference type="eggNOG" id="COG0350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_52_2_0"/>
    <dbReference type="OMA" id="IFVITTE"/>
    <dbReference type="OrthoDB" id="1327994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="SSF46767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_binding_1" evidence="6">
        <location>
            <begin position="67"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="145" mass="16853" checksum="54E7F18733B65869" modified="2009-02-10" version="1">MEKGIVNTEIGSIIVYVKDNKAIKIEFKNEFLKEIELGIFTSQIKEYFEGKRKKLLFNVEINTGKTFKKIWDFVREIPYGQTMTYGQIAKILNVNPRVVGFAMSKNPLPIYIPCHRVVGKNDIHGFTGGRKWKEYLLNLESSQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID64</accession>
    <name>B7ID64_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102004.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1500"/>
    <dbReference type="EnsemblBacteria" id="ACJ75941">
        <property type="protein sequence ID" value="ACJ75941"/>
        <property type="gene ID" value="THA_1500"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1500"/>
    <dbReference type="eggNOG" id="ENOG502Z80Y">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075784_0_0_0"/>
    <dbReference type="OMA" id="GDYDIMT"/>
    <dbReference type="OrthoDB" id="857043at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029433">
        <property type="entry name" value="DUF4438"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044909">
        <property type="entry name" value="TM_1086_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044910">
        <property type="entry name" value="TM_1086_SG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14505">
        <property type="entry name" value="DUF4438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="280" mass="30293" checksum="AEBE8332154647EB" modified="2009-02-10" version="1">MITNKEKVVKLSIMVEVAHPVVRTPVLDGEGNAFYFPGVGGITYNFGLGDNAFKMHGDHIEPDVSTKNSNDKLNSTCMTLACIGNEAEVISGDAKGMKGFVIGKHGGINHILVYFPEKEKLRVGDKIQIKAWGQGLELLDYPEVKVFNIDPELFEKLPIEEKDGKLIVPVTAEIPAHLTGSGIGSSNPAGTDYDINTHDMEEIKRYGIDKVKIGDIVAIKDHYSGFGVGGFRKGAISIGVVVHSNCVQTGHGPGIVVIMTSAKDLIVPKKVTHMNIKELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID95</accession>
    <name>B7ID95_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1531</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580264.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1531"/>
    <dbReference type="EnsemblBacteria" id="ACJ75972">
        <property type="protein sequence ID" value="ACJ75972"/>
        <property type="gene ID" value="THA_1531"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1531"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_8_4_0"/>
    <dbReference type="OMA" id="NQYVRLV"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002854448" evidence="1">
        <location>
            <begin position="19"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="2">
        <location>
            <begin position="80"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="579" mass="65961" checksum="9D4D455FC2190F11" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID99</accession>
    <name>B7ID99_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1535</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1535"/>
    <dbReference type="EnsemblBacteria" id="ACJ75976">
        <property type="protein sequence ID" value="ACJ75976"/>
        <property type="gene ID" value="THA_1535"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1535"/>
    <dbReference type="HOGENOM" id="CLU_3067157_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="53" mass="6058" checksum="9E76B99236F249D1" modified="2009-02-10" version="1">MVFSTQIPSRIAIHSTRNDMNKGNVTVKNEFRFGITEATHIEMAYNEETGEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDD6</accession>
    <name>B7IDD6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lactose transport system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1575</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102308.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1575"/>
    <dbReference type="EnsemblBacteria" id="ACJ76013">
        <property type="protein sequence ID" value="ACJ76013"/>
        <property type="gene ID" value="THA_1575"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1575"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_0"/>
    <dbReference type="OMA" id="WITDPFW"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="67"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="297" mass="33702" checksum="65A0E7D396C1F9EB" modified="2009-02-10" version="1">MKIRKSENIWGWVFISLALSGLLLFILYPIIYSLYLSTFSTRGYLKHFVGLGNYKRLFQDSNFLLSLKNIFIIFIIQVPIMLFLALIFAFLLNNPKLKFRGFYRTALFLPAVTSLVAYSVVFKMMFSTQGLVNNILLSLHIIKEPIRWLLDPFWAKVTLIIAMTWRWTGYNMMFYLAGLQNIPLEIYEAAEIDGASKTVQFFKITIPLLKPIILFTTIMSTIGTLQLFDEPMNLAAGVVTGSSVGPGNSLLTPSVYIYNVCFKYVPNFGYASAISYIIVLIAAILAIIQFKVAGDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDE0</accession>
    <name>B7IDE0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alpha-galactosidase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1579</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580280.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1579"/>
    <dbReference type="EnsemblBacteria" id="ACJ76017">
        <property type="protein sequence ID" value="ACJ76017"/>
        <property type="gene ID" value="THA_1579"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1579"/>
    <dbReference type="HOGENOM" id="CLU_905936_0_0_0"/>
    <dbReference type="OrthoDB" id="2231709at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="307" mass="36225" checksum="06FC7AE63141306B" modified="2009-02-10" version="1">MFSNFSNGYFYEKKEGFEIKWKIENFSIGWIFSWSIKGEAGIFEVFKMKKAPDLKPYSFFAINSEGLFGFLGAKEGSIEIIEDDENISFVINYEHKSFEDFSCENSFVILKHTDRGYLLKTYKNLIESQLKSKKSQFNMKDVVIFNEGLNFENLKGKVVVFNCNFEEIDKKLIENLKDLNCKIFARSKNYLQDFEYLDGFVLEGSFEENYLFSNVGNFIVLDEKEEFEGEWKVLFDNVKNNKKIVSFSCDEGKYILETINKEKRKIKIYLDFDRKVSKFAEEDFINLKSRKLIREDGRIFNFYGGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDH0</accession>
    <name>B7IDH0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1609</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580295.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1609"/>
    <dbReference type="EnsemblBacteria" id="ACJ76047">
        <property type="protein sequence ID" value="ACJ76047"/>
        <property type="gene ID" value="THA_1609"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1609"/>
    <dbReference type="eggNOG" id="ENOG50318GB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037207_0_0_0"/>
    <dbReference type="OMA" id="TKYTFQP"/>
    <dbReference type="OrthoDB" id="463273at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="454" mass="53179" checksum="F74637B7A1A2D36D" modified="2009-02-10" version="1">MKKFFLLFLILTSTIFATTFSLRYENIIYTSDTNKYFSNIFTPAIKYQNDNFSFFVEMTFSNDGKFPLFYEEFYGDYYIKFKNSGINFNLNNVKISFGNLENYDIVDSPYSLFISSLGHSKPTVSFKFEDSNFKYESRYILISYDDFVPLNLSTKSSIDKGLNLKYYAIKYGNFTFGYEEASVYVNRLFDFEYFSNPLPNFFVQYYRENVLYNGIRSFNDNSLMGFFATYEDLKKYLYAQILIDDFNANRFFNPDNPTVDKIAWSFGGTYNLNNYLKAKIFSAGATKYTFQPSADSGNPLYYGYTYFIVEKLKDWIIDYEENYIGYKYGENNLALLIGLDYSKEFNLTGDLEFILSGTKSPINPWTEKNSSTYERGFNLLNEEKIEKTFLLSLNISKKFGIMELGGFIKLGEIFNVLKLVEEVNDNVKKQYYAPSDENKKIFDLSIFLKVSYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDI4</accession>
    <name>B7IDI4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cell shape-determining protein MreB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mreB</name>
        <name evidence="3" type="ordered locus">THA_1623</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Forms polymers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="2">Membrane-associated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the FtsA/MreB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102407.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1623"/>
    <dbReference type="EnsemblBacteria" id="ACJ76061">
        <property type="protein sequence ID" value="ACJ76061"/>
        <property type="gene ID" value="THA_1623"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1623"/>
    <dbReference type="eggNOG" id="COG1077">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052037_0_0_0"/>
    <dbReference type="OMA" id="MVICIPS"/>
    <dbReference type="OrthoDB" id="410781at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10225">
        <property type="entry name" value="MreB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02207">
        <property type="entry name" value="MreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004753">
        <property type="entry name" value="MreB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01652">
        <property type="entry name" value="SHAPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00904">
        <property type="entry name" value="mreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0133">Cell shape</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="13"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="157"/>
            <end position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="205"/>
            <end position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="287"/>
            <end position="290"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="337" mass="36252" checksum="326D72F6AE2EA58E" modified="2009-02-10" version="1">MFKRYDMGIDLGTANTLVYVKNKGIVVNEPSVVAINVETDEVLKVGNEAKNMIGKTPAYIKAIRPLKDGVIADYNVALAMLSYFINRAQNGFSLFRPLVVVGVPVGITEVESRAILEAGNEAGAKRVFLIEEPMATAIGANLNVEEPTGNMVVDIGGGTTEIAVISLGSLVTWTSIRVAGDELDDAIIQYVREVYRVVIGERTAERVKIEIGNVFPDKEYDELETSVTGIDLSSGLPKKLVLKGGEIREALKPIVMQIIDSTKATLEKTPPELVADITERGIVVAGGGSLLRGITTLIEKETGINAIVADEPMTCVARGAGMVLDKISILSRLRRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDQ1</accession>
    <name>B7IDQ1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADH-ubiquinone oxidoreductase, chain 4l</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1691</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1691"/>
    <dbReference type="EnsemblBacteria" id="ACJ76128">
        <property type="protein sequence ID" value="ACJ76128"/>
        <property type="gene ID" value="THA_1691"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1691"/>
    <dbReference type="eggNOG" id="COG1006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082058_2_1_0"/>
    <dbReference type="OMA" id="KRHGTVD"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="113" mass="12316" checksum="42FEDE5A56FF2896" modified="2009-02-10" version="1">MVYYISFILIGVGIYGILTQKNLFKTLVSLTIIDTAINILIISLGYIEGSDVPIYSKYTQSANFVDPLPQALVLTAIVIGVGTLALGASLLIRVYEKYGTLDTEEIAVKEARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDS6</accession>
    <name>B7IDS6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1717</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580369.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1717"/>
    <dbReference type="EnsemblBacteria" id="ACJ76153">
        <property type="protein sequence ID" value="ACJ76153"/>
        <property type="gene ID" value="THA_1717"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1717"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047251_4_2_0"/>
    <dbReference type="OMA" id="LQTFAIM"/>
    <dbReference type="OrthoDB" id="373926at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="PNPLA" evidence="3">
        <location>
            <begin position="5"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="36"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="163"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="264" mass="30355" checksum="7362F7D205CF8C2F" modified="2009-02-10" version="1">MKIGLSLAAGGVKGFAHIATLKFLEENDIDVDIITGSSAGAIVAALYGLYGDSNKVFEMFSEAIKLYLPNFKKYMTKLEKSNFWYIFQRSLVPVEDYYPFFKFLFGKKKFSDLKKNVGIITFDTLEGTSLLITEGFLVDAVMASSSVPGVFSPLWIAGTQNTDGGVLAPTPVKEAKLLGSDFVVASTFERKPQDFPKDQLELMYYLDTWKEIEIEYVDLELANVVIYYRIPYQWYEFNNYLQIYNTALNYINERREEFDPRFWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDU3</accession>
    <name>B7IDU3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bifunctional protein PyrR</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="4">Pyrimidine operon regulatory protein</fullName>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="4">Uracil phosphoribosyltransferase</fullName>
                <shortName evidence="4">UPRTase</shortName>
                <ecNumber evidence="4">2.4.2.9</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="4" type="primary">pyrR</name>
        <name evidence="6" type="ordered locus">THA_1735</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Also displays a weak uracil phosphoribosyltransferase activity which is not physiologically significant.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Regulates the transcription of the pyrimidine nucleotide (pyr) operon in response to exogenous pyrimidines.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>diphosphate + UMP = 5-phospho-alpha-D-ribose 1-diphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:13017"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrR subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580378.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1735"/>
    <dbReference type="EnsemblBacteria" id="ACJ76170">
        <property type="protein sequence ID" value="ACJ76170"/>
        <property type="gene ID" value="THA_1735"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1735"/>
    <dbReference type="eggNOG" id="COG2065">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094234_2_1_0"/>
    <dbReference type="OMA" id="PIQPDFC"/>
    <dbReference type="OrthoDB" id="1725260at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01219">
        <property type="entry name" value="PyrR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023050">
        <property type="entry name" value="PyrR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="5">
        <location>
            <begin position="13"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="PRPP-binding" evidence="4">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="176" mass="20072" checksum="817F9958D9A8B389" modified="2009-02-10" version="1">MKKILGKDDIRRSLMRISHEILERNKGAEDIVLLGIYTRGYYLAKRISENIKMIEGVAVPVGGLDVGPFRDDEKKTKDDKSNINFEVKNKIVILVDDVLFTGRTIRAAMDAVTSRGRPKFIQLAVLVDRGHREFPIRPDYVGKNIPSSKERETIKVRISEVDGEDSVYILDKEEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDU6</accession>
    <name>B7IDU6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptide chain release factor N(5)-glutamine methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.297</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1738</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine = H(+) + N(5)-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42896"/>
            <dbReference type="Rhea" id="RHEA-COMP:10271"/>
            <dbReference type="Rhea" id="RHEA-COMP:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
            <dbReference type="EC" id="2.1.1.297"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.297" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102631.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1738"/>
    <dbReference type="EnsemblBacteria" id="ACJ76173">
        <property type="protein sequence ID" value="ACJ76173"/>
        <property type="gene ID" value="THA_1738"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1738"/>
    <dbReference type="eggNOG" id="COG2890">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_3_2_0"/>
    <dbReference type="OMA" id="SYEPQNA"/>
    <dbReference type="OrthoDB" id="1816476at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102559">
        <property type="term" value="F:protein-(glutamine-N5) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019874">
        <property type="entry name" value="Release_fac_Glu-N5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03534">
        <property type="entry name" value="RF_mod_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS" evidence="7">
        <location>
            <begin position="96"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="261" mass="30272" checksum="1E23F83E4A1EF69A" modified="2009-02-10" version="1">MTVYEALKIYKDRGLPELEAYLVIKKITGKTKEYLVAHPEYQFCEQSFFDLLNKRLVGFPLSYLLNEKSFYKHNFYVEEGVLIPRPETELLVENALKVIDKEKIKKVAEIGVGSGAVIISILLETNCVGFGTDISKKALEIAKKNARKFRVIDRLDLRLGKFLEPFKNEYDEIELIVSNPPYVKNNSNLQKEVMFEPKEALFGGEDGLDFYREFLSSYDLNGKIVVMEIGHDQGDFFRKKDWNVIKDYSGNDRIVIKDFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE27</accession>
    <name>B7IE27_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1823</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580446.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1823"/>
    <dbReference type="EnsemblBacteria" id="ACJ76254">
        <property type="protein sequence ID" value="ACJ76254"/>
        <property type="gene ID" value="THA_1823"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1823"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_0"/>
    <dbReference type="OMA" id="SFFKWDF"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="202"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="429" mass="50353" checksum="100CD67298FFDF6F" modified="2009-02-10" version="1">MKTATKKKLKESLLAYLFLLPSLIVLGIFVFWPVGFSFVLSFFKWDFRNMNAPYFNGLDNYISIFEFDYPPKYSFFESFIFSIGYILIAILIVITFYTLITVFSKKKNIFLLLLNVIPIILIPFFKNMNFYLMIFFDIVIFILVFYNFKNREQMRLYRNYTWLNVAAIIFTYVMIEASKFNENGLLNFFMDAKDKNLFVKALVNTFYYVILTVPITLAISLFLAVLLNSNIRFKVFFRTSYFIPFVTSVVAVSLVWKWIFNDEFGLLNYFLSWFGIEPIRWLKDEKWTIPTIAIVSIWKQIGYDAIIFLAGLQNIDQFYYEAAEVDGANSWHKFSKITWPLLSPTTFFLLIVSMIGAFKVFAQVYVLYDGLPGPYNNSGMTMVYYVFDLFYRQQRMGIASAAAYLLFGIILIFTAIQYKVGNKVVEYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE49</accession>
    <name>B7IE49_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1845</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580458.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1845"/>
    <dbReference type="EnsemblBacteria" id="ACJ76276">
        <property type="protein sequence ID" value="ACJ76276"/>
        <property type="gene ID" value="THA_1845"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1845"/>
    <dbReference type="HOGENOM" id="CLU_2208801_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="107" mass="12908" checksum="BE83EF6C1C772903" modified="2009-02-10" version="1">MEEMFEVEETLKKINSEVKKLKTYNDSIEVIEKSLSEILNSYKRTLSKLDEYIKINNDLETQIKQLNLKLEKMNDDFFKAKRFLKDNIFEYSIIIVFIVFLIFEILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE92</accession>
    <name>B7IE92_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Hypoxanthine phosphoribosyltransferase</fullName>
            <ecNumber evidence="1 2">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1893</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>diphosphate + IMP = 5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="1 2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102901.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1893"/>
    <dbReference type="EnsemblBacteria" id="ACJ76319">
        <property type="protein sequence ID" value="ACJ76319"/>
        <property type="gene ID" value="THA_1893"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1893"/>
    <dbReference type="eggNOG" id="COG0634">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073615_0_1_0"/>
    <dbReference type="OMA" id="TMDWMAV"/>
    <dbReference type="OrthoDB" id="1819460at2"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052657">
        <property type="term" value="F:guanine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005904">
        <property type="entry name" value="Hxn_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01203">
        <property type="entry name" value="HGPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="3">
        <location>
            <begin position="13"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU364099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="170" mass="19532" checksum="A45EF47332DEF920" modified="2009-02-10" version="1">MNLNVLIGEQKLQERIKSLGKEITEYYKEKTDTIHAVCVLKGAIHFFTDLVENIDLNVEYSFIHVSSYSGMESTGRIRVKSWIDEPIEGRHVLVVEDILDTGQTLSYILGYLKRYNPADLKVATLLKKTSKNPIVNAEFVGFEIEDKFVVGYGLDFDEKYRNIPYIGYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEA3</accession>
    <name>B7IEA3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ion-translocating oxidoreductase complex subunit D</fullName>
            <ecNumber evidence="10">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Rnf electron transport complex subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rnfD</name>
        <name evidence="11" type="ordered locus">THA_1904</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NqrB/RnfD family.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580476.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1904"/>
    <dbReference type="EnsemblBacteria" id="ACJ76330">
        <property type="protein sequence ID" value="ACJ76330"/>
        <property type="gene ID" value="THA_1904"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1904"/>
    <dbReference type="eggNOG" id="COG4658">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042020_1_0_0"/>
    <dbReference type="OMA" id="GWQWINL"/>
    <dbReference type="OrthoDB" id="1654433at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00462">
        <property type="entry name" value="RsxD_RnfD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004338">
        <property type="entry name" value="NqrB/RnfD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011303">
        <property type="entry name" value="RnfD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30578">
        <property type="entry name" value="PTHR30578"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30578:SF0">
        <property type="entry name" value="PTHR30578:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03116">
        <property type="entry name" value="NQR2_RnfD_RnfE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01946">
        <property type="entry name" value="rnfD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 10" id="KW-0288">FMN</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="modified residue" description="FMN phosphoryl threonine" evidence="10">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ76330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="318" mass="34864" checksum="D857EBC784762F20" modified="2009-02-10" version="1">MKLITGNAPHLRSTDTTPRVMLDVIIALLPALIGAWYFFGFYAFFLAILGAFFGELFELFIMKVLRKDKNFVPNGSAAVTGILLAMNVSPATSWWVFLLGLVFALGVAKHAFGGLGMNIFNPALAGRAFLLVSFPVQMTTWYKPTLSFSKWVDVQTTATPLAVLKETGSVNVSYWDLFIGNRAGSIGETSVLLLLIGFAYLVARKRIKLMIPIFYIGTVFVMSSFFYFANPTFGTPLFHILSGGLMLGALFMATDMVTSPMTIKGQAIFGIGAGVMTLLIRYFAGYPEGVSLSILLMNAFVPLIDRYTQPRIFGEVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEA5</accession>
    <name>B7IEA5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ion-translocating oxidoreductase complex subunit E</fullName>
            <ecNumber evidence="7">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Rnf electron transport complex subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rnfE</name>
        <name evidence="8" type="ordered locus">THA_1906</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NqrDE/RnfAE family.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102925.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1906"/>
    <dbReference type="EnsemblBacteria" id="ACJ76332">
        <property type="protein sequence ID" value="ACJ76332"/>
        <property type="gene ID" value="THA_1906"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1906"/>
    <dbReference type="eggNOG" id="COG4660">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046659_1_1_0"/>
    <dbReference type="OMA" id="DGFMMGL"/>
    <dbReference type="OrthoDB" id="1782047at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00478">
        <property type="entry name" value="RsxE_RnfE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003667">
        <property type="entry name" value="NqrDE/RnfAE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010968">
        <property type="entry name" value="RnfE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30586:SF0">
        <property type="entry name" value="PTHR30586:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02508">
        <property type="entry name" value="Rnf-Nqr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006102">
        <property type="entry name" value="NQR_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01948">
        <property type="entry name" value="rnfE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="204" mass="22177" checksum="023790E4A8700DAD" modified="2009-02-10" version="1">MASRSWKEFSKGFIVENPTYVQALGMCPTLATTTSAKNGLGMGLAATAVLVMSNIVVSLLRKAVPEKIRIPIFITIIASFVTIVDLLMHAYVYDLWKTLGLFIPLIVVNCVIMGRAESFASKNNVWYSMLDGLGMGLGFTASLTLLGAIRELLGSGTLFDVKIWGKAFNVFIMILPPGAYLTLGLLAALFAYIGMKRKERGNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEA6</accession>
    <name>B7IEA6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ion-translocating oxidoreductase complex subunit A</fullName>
            <ecNumber evidence="8">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Rnf electron transport complex subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnfA</name>
        <name evidence="9" type="ordered locus">THA_1907</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NqrDE/RnfAE family.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102926.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1907"/>
    <dbReference type="EnsemblBacteria" id="ACJ76333">
        <property type="protein sequence ID" value="ACJ76333"/>
        <property type="gene ID" value="THA_1907"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1907"/>
    <dbReference type="eggNOG" id="COG4657">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095255_1_0_0"/>
    <dbReference type="OMA" id="CPFFGVS"/>
    <dbReference type="OrthoDB" id="1814639at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00459">
        <property type="entry name" value="RsxA_RnfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011293">
        <property type="entry name" value="Ion_transpt_RnfA/RsxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003667">
        <property type="entry name" value="NqrDE/RnfAE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02508">
        <property type="entry name" value="Rnf-Nqr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006102">
        <property type="entry name" value="NQR_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01943">
        <property type="entry name" value="rnfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="191" mass="21117" checksum="AD37A6C673A58D9F" modified="2009-02-10" version="1">MKIFLILLSAMLVNNYVFMRFLGICPFLGVSKKMDTAVGMGLAATFVLVMSSTISWFVDRLLISLGLEFLRTIAFILVIASFVQFVELFLKKNNPNLYDALGIFLPLITTNCIILGVALINSMNNYNLLETIFNSLGAGLGFLLALIIFSAIREKLELYDLPKPFEGLPIALITASLLSLAFMGFQGMIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEA9</accession>
    <name>B7IEA9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1911</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102928.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1911"/>
    <dbReference type="EnsemblBacteria" id="ACJ76336">
        <property type="protein sequence ID" value="ACJ76336"/>
        <property type="gene ID" value="THA_1911"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1911"/>
    <dbReference type="HOGENOM" id="CLU_946398_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="294" mass="34678" checksum="AE4BD06FA601FD00" modified="2009-02-10" version="1">MPIYRVNDVTNDERINDYLKLNEKDDIKGISFGVLPQKETLFPSIEDIVYDSPDEIIETLEEADFSGCEGDAGDCVMNFLESLSDEDYVKFLRNFIINFNEVYSEIVYFNYDEVLSDEDIKDFLLENIESIIEECHYKELDDEEDESYLFEQLDDDEMLETIEEFSGDIESIKIGSLDALFWGNEEKLSKETKEILEKLDAERYEINRDLVVVYTNYFMENFAEKVGEILVEIFEDGDWPEEIWTRNAVNRMFNLAFDKIWQHMDEIESYIAENLSNDYLYALSRAFVATLEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEC8</accession>
    <name>B7IEC8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar ABC transporter permease, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1932</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102984.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1932"/>
    <dbReference type="EnsemblBacteria" id="ACJ76355">
        <property type="protein sequence ID" value="ACJ76355"/>
        <property type="gene ID" value="THA_1932"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1932"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_0"/>
    <dbReference type="OMA" id="FPYLING"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="157"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="68"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="294" mass="33194" checksum="13BA95088A5BDEB9" modified="2009-02-10" version="1">MKKIKKYMHWILLSPYLTLFIIFILLPIIVAIYLSFTYFNAIQSPRWIGLQNYVNLFTRDEIFMQKVLPNTIKFSLIVGPGGYILSFILAWLVAQLTKVPRTIFALILYSPSLTAGVTMAVVWRVLFNGDQQGYLNALLLKLGFIDKPIQWLQSPEYLLTIMIIVSLWSSMGVGFLAMLAGILNVDETLYEAAYIDGIKNRLQEIIYITIPSMKPQMLFGAVMSIVQTFTTAGIGVALSGTNPTPQYAGQLIVNHIEDYGFLRYEMGYAAAVSVVLLIIIYLFSKIAWKLFGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEE8</accession>
    <name>B7IEE8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1953"/>
    <dbReference type="EnsemblBacteria" id="ACJ76375">
        <property type="protein sequence ID" value="ACJ76375"/>
        <property type="gene ID" value="THA_1953"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1953"/>
    <dbReference type="HOGENOM" id="CLU_3104876_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="51" mass="6031" checksum="56699295451C8884" modified="2009-02-10" version="1">MCGSVKKPGDRLKKIKKLCNFKIFYVNIVYFFAMIFSKRLQKVIECNIIIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEF3</accession>
    <name>B7IEF3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cas crispr-associated protein Cas5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1958</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580506.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1958"/>
    <dbReference type="EnsemblBacteria" id="ACJ76380">
        <property type="protein sequence ID" value="ACJ76380"/>
        <property type="gene ID" value="THA_1958"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1958"/>
    <dbReference type="eggNOG" id="ENOG502ZBNI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100173_0_0_0"/>
    <dbReference type="OMA" id="TYNNIHK"/>
    <dbReference type="OrthoDB" id="1815464at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021124">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013422">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09704">
        <property type="entry name" value="Cas_Cas5d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02593">
        <property type="entry name" value="CRISPR_cas5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="257" mass="30449" checksum="8610CF7BE232FDED" modified="2009-02-10" version="1">MKAFCFNIKADMGFFKKNDSNARVFTTYNFIHKPAVLGIFGAILGYSGYDRDRKNNGIEYYEKLKNFKIAIKPLYKKPLKKTVVVFNNSSGLASEETGGVLQAKEQVIVESLGYTIFVPFFETFSEAEKKIYEKLEYMVKNKYAEYPIYFGKNEFFAYFENYRSLNVEELERKEAVVDSLVKMKDVDFFGKTDEEEFDLFEEHKEEQKITLIEELPYELDENYMYVKDLFIFTDKELKIKNNEGFWRTIDDEIIYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEM6</accession>
    <name>B7IEM6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide transport system permease protein AppC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_32</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579306.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_32"/>
    <dbReference type="EnsemblBacteria" id="ACJ74540">
        <property type="protein sequence ID" value="ACJ74540"/>
        <property type="gene ID" value="THA_32"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_32"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_2_0"/>
    <dbReference type="OMA" id="FIRSVYI"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="100"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="304" mass="33435" checksum="357BEB1623C42944" modified="2009-02-10" version="1">MNTEFKRTMKKLFRNPSAVLGFSLLIFFALIAAFAPLLAPPVNPMFPDENGNPMKLEDPYIMPILSWSSDPVPPSKDHPFGMIAGRDIYYGVIWGTRTAFKIGLTVVTLSTIIGLIIGSIAGYFGGWIDEILMRITDIFLSIPFLLAAMVLTTILGSGLDKVMIAMTVFGWMAVARLIRANILQVKEEQFVLAAKALGIKNFIIIVRHVLPNTIFPVLVQATMRLGSLVITAAALSFLGLGAPIGYADWGSMLNYTRNYILGASGQALAYWYTVFYPGMAMVLFVLAWNLVGDALRDVFDPKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEN8</accession>
    <name>B7IEN8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_44</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103372.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_44"/>
    <dbReference type="EnsemblBacteria" id="ACJ74552">
        <property type="protein sequence ID" value="ACJ74552"/>
        <property type="gene ID" value="THA_44"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_44"/>
    <dbReference type="HOGENOM" id="CLU_1757953_0_0_0"/>
    <dbReference type="OMA" id="YLRNDKQ"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="154" mass="17666" checksum="65C81B182F4DC534" modified="2009-02-10" version="1">MTLNKKYLFIILVLILIVVIFFISILNLRKSLEVEVLSSVNVVKIKDYYLNFINVKDVSFFEPYYLKIDGSYSKLGNFSIVEDLEYLGYYDESGTSVVYLRNDKQLLKVDPKQILLNRYYILNISGNSLVVLDIKDGSIKLIVSKKSGGISDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEP4</accession>
    <name>B7IEP4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cobalt import ATP-binding protein CbiO 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_50</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103375.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_50"/>
    <dbReference type="EnsemblBacteria" id="ACJ74558">
        <property type="protein sequence ID" value="ACJ74558"/>
        <property type="gene ID" value="THA_50"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_50"/>
    <dbReference type="eggNOG" id="COG1122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_0"/>
    <dbReference type="OMA" id="MFQETEL"/>
    <dbReference type="OrthoDB" id="1752365at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="220" mass="25549" checksum="82F90C1796FB5630" modified="2009-02-10" version="1">MTLELKNVNYIYNYKTVFQKHVLKDINFKINDNEVIFIIGKSGSGKTTLLYLMDFLLKPTFGEIVVDGEDPFTNPHKFRKKIGFAFQFPEKQFFSETVEDEIMFSIKNFEIKNPKERLEEIKDLMNLDDELLKKSPFQLSGGQQRKVAIASAIAHDPELLILDEPTVSLDYKSEKSLIEFLKLWASKKDKSLIIVTHDLEKFSDFKGKIFEIVDGEGYFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IER7</accession>
    <name>B7IER7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pilin secretion/fimbrial assembly signal peptidase, PilD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_73</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579332.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_73"/>
    <dbReference type="EnsemblBacteria" id="ACJ74581">
        <property type="protein sequence ID" value="ACJ74581"/>
        <property type="gene ID" value="THA_73"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_73"/>
    <dbReference type="eggNOG" id="COG1989">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057101_0_1_0"/>
    <dbReference type="OMA" id="VFWLFKL"/>
    <dbReference type="OrthoDB" id="2046608at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010627">
        <property type="entry name" value="Pept_A24A_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06750">
        <property type="entry name" value="DiS_P_DiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="DiS_P_DiS" evidence="2">
        <location>
            <begin position="8"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="232" mass="27348" checksum="5F57B0B79125BDE1" modified="2009-02-10" version="1">MWYFLNFLLGIILGSFLNVIIYRSVEGIKIYDPPRSFCPMCNHQLKWYDNIPLLSYLLLRGKCRYCGSKIPFRYFFVECIVGILFLIHSILFNYTEAILLNIIVFAIIPAIFIDFSIMMIPDFSWILIWVVSVIDLLLRHELWFLKTFSFITIMIIFMIIKKIYKEGLGEGDIYLIAPFSFLLIPPLSIYLLLFSSISALIYALIKKNKIIPFGPFISISGYFLYFFMLKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IES3</accession>
    <name>B7IES3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_79</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579337.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_79"/>
    <dbReference type="EnsemblBacteria" id="ACJ74587">
        <property type="protein sequence ID" value="ACJ74587"/>
        <property type="gene ID" value="THA_79"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_79"/>
    <dbReference type="HOGENOM" id="CLU_2119978_0_0_0"/>
    <dbReference type="OMA" id="HIINIMI"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="114" mass="13501" checksum="8F5A194D2AC654BB" modified="2009-02-10" version="1">MAHIINIMIGFLFLSTFFGLENYHISIIISIFIYIIINPGKFNFLKVFYKLVINIPKSLYETLILPILKKEVIESEKYTDDFQMLIKILTITLTPKTIVFDHDNNFLYIHKLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IET0</accession>
    <name>B7IET0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metallophosphoesterase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_86</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579341.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_86"/>
    <dbReference type="EnsemblBacteria" id="ACJ74594">
        <property type="protein sequence ID" value="ACJ74594"/>
        <property type="gene ID" value="THA_86"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_86"/>
    <dbReference type="eggNOG" id="COG1409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082706_0_0_0"/>
    <dbReference type="OMA" id="RINDWAD"/>
    <dbReference type="OrthoDB" id="1242748at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="Metallophos" evidence="2">
        <location>
            <begin position="68"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="283" mass="33518" checksum="87D0A7B667076DE4" modified="2009-02-10" version="1">MTFKKYVIFFLIIFSIVIFPEINKTLYFNFNNKIIVKNYDESFNFSLNESTIVAIYGDSRWGDKIHSEIVSQILKFNPHVVIHLGDMVNKGDNINDWEKFFEITYDLRKRSYFQVVKGNHENPSDYFDKIFGVKNYYSDFFGYRFIFLDAESGINKCIEFIKNYANEKSIVFLHYPIFTVGPHFKDSIVKKLKILHEIFLNKNVKLVISAHEHNYQHFVFNNFHYIITGGGGAATYNKVLNNKNLTFFSKVHNFVILEFNNKFVTVKSYKYNGELLEEFNVQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IET3</accession>
    <name>B7IET3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_89</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103447.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_89"/>
    <dbReference type="EnsemblBacteria" id="ACJ74597">
        <property type="protein sequence ID" value="ACJ74597"/>
        <property type="gene ID" value="THA_89"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_89"/>
    <dbReference type="eggNOG" id="ENOG502ZRWA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1102367_0_0_0"/>
    <dbReference type="OMA" id="LFMDFWN"/>
    <dbReference type="OrthoDB" id="1919817at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="252" mass="30171" checksum="B713D21A3093F428" modified="2009-02-10" version="1">MEIYIDSTFSKNELIEFVVPIIIRANNNFFVDFYNYIALYNPNLNLVKNEKLLDIHMNVYEINTEIDFDNLQELTKENSEIFFTRNFIAKKALELKINENNNFLRYENSISSIELIISLNTNFLIDNPEIFSYKNSEKIFDLFIYIKLLYLSKKYNYLLESKGSTMIYKINYQPDFNLFMDFWNNFVKLNSKLTPTFLNRISNKTCEKIKNVFTFLPQDTLKELVLKDELFINKLFNEITTLKRIISEEVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEW9</accession>
    <name>B7IEW9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_125</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3 6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_125"/>
    <dbReference type="EnsemblBacteria" id="ACJ74633">
        <property type="protein sequence ID" value="ACJ74633"/>
        <property type="gene ID" value="THA_125"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_125"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_3_6_0"/>
    <dbReference type="OMA" id="RHGKRWM"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:cellular aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="PTHR43570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="7">
        <location>
            <begin position="9"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="active site" evidence="4 5">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="445" mass="51031" checksum="E7C0634DF4716A33" modified="2009-02-10" version="1">MINSLKEFFSTGKTYDLEFRINQLKILKHALEEFEEDLYSALFLDLNKSKEESFLTEIFIVYKEIKYFIKNLKKLSKPKRVKVGIENQSGKGFLIPEPYGVALIISPWNYPVNLTLTPLVGAIAAGNCAVLKPSEYSENVTKVLEKMITKYFSEDYIKVVTGDAEISKKLLDQDFDYIFFTGSPGVGKYVMKKASERLIPVTLELGGKNPTIVDSTCDLELSAKRIAWGKLLNAGQTCISPDYLIVEESIKEQLVERLIKYFEDFQSKMACIINDRHVKRLKNLLNNTKGRVIFGGKVYERKFEPTIVDDVKINDSLMSEEIFGPILPIITFKDEKDIFEIISKNPYPLSLYVFSNNKVFVKNVINKIQAGGISVNDTISHFVPESFPFGGIKTSGIGRYHGRYSFETFSHLKPVFFRGKFEINVKYQPYKGFEKIQKILRKWYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEX8</accession>
    <name>B7IEX8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA-binding response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_134</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103564.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_134"/>
    <dbReference type="EnsemblBacteria" id="ACJ74642">
        <property type="protein sequence ID" value="ACJ74642"/>
        <property type="gene ID" value="THA_134"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_134"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_0"/>
    <dbReference type="OMA" id="LAEVWDW"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="5"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="135"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="135"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="239" mass="27873" checksum="A52555C6C707BB16" modified="2009-02-10" version="1">MGKRRIMVIDDQPEILELISFTLEKEGYEVVPVEDAEKALEEIKDKEIDMFLVDIMLPGMDGFEFVRNIRSQEKYKSTPVIFLSAKGEEFDKVLGLELGADDYITKPFSIRELLARIKAVFRRMQLSTQVKEEKPKKIVAKDLEIDVDKYEVKIKGKKVNLTPLEFDLLRFLAENEGKVFSRNVLLDKLWGYDYFGDTRTVDVHIRRLRTKIEEDPSNPKYIVTVRGKGYKFRDPGKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEY3</accession>
    <name>B7IEY3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glucose-6-phosphate isomerase</fullName>
            <shortName evidence="7">GPI</shortName>
            <ecNumber evidence="7">5.3.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Phosphoglucose isomerase</fullName>
            <shortName evidence="7">PGI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphohexose isomerase</fullName>
            <shortName evidence="7">PHI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pgi</name>
        <name evidence="9" type="ordered locus">THA_139</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7 8">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7 8">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103575.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_139"/>
    <dbReference type="EnsemblBacteria" id="ACJ74647">
        <property type="protein sequence ID" value="ACJ74647"/>
        <property type="gene ID" value="THA_139"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_139"/>
    <dbReference type="eggNOG" id="COG0166">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037303_1_0_0"/>
    <dbReference type="OMA" id="IGVWYIN"/>
    <dbReference type="OrthoDB" id="417261at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="PTHR11469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 8" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4 7 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="449" mass="50852" checksum="B081AEC003F1C2F2" modified="2009-02-10" version="1">MLKFDFTNLFSNKVPNNITLDEVKSYENKINQIIKEIDTNRPGFVETIFSRKWIDSVLDLKDTIFQFDNLVVLGIGGSALGNIALQNTLKPLNWNFLSKEERNRKLRIFVIDNVDPDFVASILDTIDIRKTLFNVISKSGSTAEAMSNYLIARGIIETYGLDPKKHLIFTTDPEKGVLRKIANEENIKTLEIPQNVGGRFSVLTPVGLLSAYAAGINIEALYDGAKIAYSRFLKPLFENPAALIAITHYLYYNKGRNISVMMAYSNRLYHLADWYRQLWAESLGKKLDKDGNIVNVGQTPVKALGAVDQHSQVQLYNEGPDDKIITFLKTEKFNRDIKIPNVHEEEALSYLQGKYLSELLNNEQLGTESAILEHGKPSLKITFPVIDEVHVGEFFMHYELATAIAGELFNINPYDQPGVELGKKITYALMGRPGYDDHKINIEEKRIEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF03</accession>
    <name>IF2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">infB</name>
        <name type="ordered locus">THA_160</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579381.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IF03"/>
    <dbReference type="SMR" id="B7IF03"/>
    <dbReference type="STRING" id="484019.THA_160"/>
    <dbReference type="EnsemblBacteria" id="ACJ74667">
        <property type="protein sequence ID" value="ACJ74667"/>
        <property type="gene ID" value="THA_160"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_160"/>
    <dbReference type="eggNOG" id="COG0532">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006301_5_1_0"/>
    <dbReference type="OMA" id="NRDNRTG"/>
    <dbReference type="OrthoDB" id="347113at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006847">
        <property type="entry name" value="IF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="PTHR43381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04760">
        <property type="entry name" value="IF2_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="SSF52156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00487">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000117338" description="Translation initiation factor IF-2">
        <location>
            <begin position="1"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="187"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="196"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="221"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="242"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="296"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="333"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="196"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="242"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="296"/>
            <end position="299"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <sequence length="696" mass="78610" checksum="9E3E3DBDB8DF8DC4" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF16</accession>
    <name>B7IF16_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aspartokinase</fullName>
            <ecNumber evidence="13">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">THA_175</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-aspartate = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 14">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 13">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="13"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579390.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_175"/>
    <dbReference type="EnsemblBacteria" id="ACJ74680">
        <property type="protein sequence ID" value="ACJ74680"/>
        <property type="gene ID" value="THA_175"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_175"/>
    <dbReference type="eggNOG" id="COG0527">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_3_2_0"/>
    <dbReference type="OMA" id="DMIVQTI"/>
    <dbReference type="OrthoDB" id="1067792at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04261">
        <property type="entry name" value="AAK_AKii-LysC-BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041740">
        <property type="entry name" value="AKii-LysC-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13840">
        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 13 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="15">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="ACT_7" evidence="16">
        <location>
            <begin position="336"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="6"/>
            <end position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="207"/>
            <end position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000726-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF13840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ACJ74680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="399" mass="44210" checksum="5082C1204728AC07" modified="2009-02-10" version="1">MIVVQKYGGSSVADVEKILKVAKRIKKRIEKGDKIIVIVSAMGKTTDNLINLAKSLSKNPHPREMDMLLTTGEQMSVALLSIALNELGIKSKSLNAFQLNIKTTTDHTKARIIDIDIDKLNNELQKNEVIIVTGFQGITEDGDLTTLGRGGSDTSAVAIAAKLNVNCEIYSDVDGIYTCDPRIVKSAKKLKYITYDDMLELSSLGAKVLHSRAVELAKKYNIKIYCASTFSEEEGTWVVDKLPEWLEQPVVTGATIEKDQIKITLNNLPKDKKIIEKIFETLSNNKINIDMISMFADNEKFHLSFSVLNDLRETIEKSIKAANPLIEMSYQGMFDKVSIVGVGMKSSPGVAARFFKVLYENNIIPELVTTSEIKISVLVSKEKSELLLKELAKEFSLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF34</accession>
    <name>B7IF34_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA polymerase III, gamma subunit-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579402.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_193"/>
    <dbReference type="EnsemblBacteria" id="ACJ74698">
        <property type="protein sequence ID" value="ACJ74698"/>
        <property type="gene ID" value="THA_193"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_193"/>
    <dbReference type="eggNOG" id="COG0470">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1000218_0_0_0"/>
    <dbReference type="OMA" id="LWICEEF"/>
    <dbReference type="OrthoDB" id="1971388at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="265" mass="30762" checksum="8B4FF829858F5159" modified="2009-02-10" version="1">MEKIRKLIENNSGNSIAIVGENKRYVNDKVMQAVLDVTNYDIQRYLMIDENNIKIDHVRNIQEFLSFSSDKGKKIVVILNAEKMLPEAENALLKTLEEPPSYSLIVLTTTNWKALYPTIRSRLQKYYVSVPKDILLGLQDFILKKLAIEFPDKVDEIKANNLKIVEIENFEESPSYYYSLYLKIKENLEDAKKINNFVASLSSIKDFNFLKVLSKIALWICEEFSCDYKFAKVLSSILSSKVANYNYELTYYFILLSLNDAIKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF38</accession>
    <name>B7IF38_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Putative K(+)-stimulated pyrophosphate-energized sodium pump</fullName>
            <ecNumber evidence="9">7.2.3.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Membrane-bound sodium-translocating pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Pyrophosphate-energized inorganic pyrophosphatase</fullName>
            <shortName evidence="9">Na(+)-PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hppA</name>
        <name evidence="10" type="ordered locus">THA_197</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na(+) movement across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>diphosphate + H2O + Na(+)(in) = H(+) + Na(+)(out) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:57884"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Requires K(+) for maximal activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.3.-" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103705.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_197"/>
    <dbReference type="EnsemblBacteria" id="ACJ74702">
        <property type="protein sequence ID" value="ACJ74702"/>
        <property type="gene ID" value="THA_197"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_197"/>
    <dbReference type="eggNOG" id="COG3808">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008743_3_1_0"/>
    <dbReference type="OMA" id="MATTAMQ"/>
    <dbReference type="OrthoDB" id="98036at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009678">
        <property type="term" value="F:pyrophosphate hydrolysis-driven proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01129">
        <property type="entry name" value="PPase_energized_pump"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004131">
        <property type="entry name" value="PPase-energised_H-pump"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31998">
        <property type="entry name" value="PTHR31998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03030">
        <property type="entry name" value="H_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001265">
        <property type="entry name" value="H+-PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01104">
        <property type="entry name" value="V_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 9" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="344"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="539"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="563"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="630"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="site" description="Determinant of potassium dependence" evidence="9">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="648" mass="67735" checksum="C3D963AF0876F848" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF88</accession>
    <name>B7IF88_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">ATP-dependent DNA helicase RecG</fullName>
            <ecNumber evidence="3 6">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">recG</name>
        <name evidence="9" type="ordered locus">THA_250</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the helicase family. RecG subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_250"/>
    <dbReference type="EnsemblBacteria" id="ACJ74752">
        <property type="protein sequence ID" value="ACJ74752"/>
        <property type="gene ID" value="THA_250"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_250"/>
    <dbReference type="eggNOG" id="COG1200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005122_7_1_0"/>
    <dbReference type="OMA" id="DNGFQAC"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004609">
        <property type="entry name" value="ATP-dep_DNA_helicase_RecG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045562">
        <property type="entry name" value="RecG_dom3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028993">
        <property type="entry name" value="RecG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036845">
        <property type="entry name" value="RecG_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033454">
        <property type="entry name" value="RecG_wedge"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19833">
        <property type="entry name" value="RecG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17190">
        <property type="entry name" value="RecG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17191">
        <property type="entry name" value="RecG_wedge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69008">
        <property type="entry name" value="SSF69008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00643">
        <property type="entry name" value="recG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="361"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="541"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="769" mass="88510" checksum="F4631DA7C7A5E3FE" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFA6</accession>
    <name>CH60_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperonin GroEL</fullName>
            <ecNumber evidence="1">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">60 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Chaperonin-60</fullName>
            <shortName evidence="1">Cpn60</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">groEL</name>
        <name evidence="1" type="synonym">groL</name>
        <name type="ordered locus">THA_269</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide.</text>
            <dbReference type="EC" id="5.6.1.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back. Interacts with the co-chaperonin GroES.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579459.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFA6"/>
    <dbReference type="SMR" id="B7IFA6"/>
    <dbReference type="STRING" id="484019.THA_269"/>
    <dbReference type="EnsemblBacteria" id="ACJ74770">
        <property type="protein sequence ID" value="ACJ74770"/>
        <property type="gene ID" value="THA_269"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_269"/>
    <dbReference type="eggNOG" id="COG0459">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016503_3_0_0"/>
    <dbReference type="OMA" id="TDTDKME"/>
    <dbReference type="OrthoDB" id="265347at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="SSF54849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000130069" description="Chaperonin GroEL">
        <location>
            <begin position="1"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="86"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="479"/>
            <end position="481"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="495"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00600"/>
        </source>
    </evidence>
    <sequence length="539" mass="58080" checksum="531D5230FD7E66BB" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFA8</accession>
    <name>B7IFA8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mini-ribonuclease 3</fullName>
            <shortName evidence="2">Mini-3</shortName>
            <shortName evidence="2">Mini-RNase 3</shortName>
            <ecNumber evidence="2">3.1.26.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Mini-RNase III</fullName>
            <shortName evidence="2">Mini-III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mrnC</name>
        <name evidence="4" type="ordered locus">THA_272</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in correct processing of both the 5' and 3' ends of 23S rRNA precursor. Processes 30S rRNA precursor transcript even in absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller rRNA precursors.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MrnC RNase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.-" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579461.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_272"/>
    <dbReference type="EnsemblBacteria" id="ACJ74772">
        <property type="protein sequence ID" value="ACJ74772"/>
        <property type="gene ID" value="THA_272"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_272"/>
    <dbReference type="eggNOG" id="COG1939">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091169_3_0_0"/>
    <dbReference type="OMA" id="AYMGDAI"/>
    <dbReference type="OrthoDB" id="1939897at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01468">
        <property type="entry name" value="RNase_Mini_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008226">
        <property type="entry name" value="Mini3_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005520">
        <property type="entry name" value="UCP005520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="SSF69065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="3">
        <location>
            <begin position="20"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="126" mass="14462" checksum="D734C302C7393195" modified="2009-02-10" version="1">MNIFEKIHPNLNVNELSTDSLAYLGDAVFNLFVKVLYFKNTSVKNLHKDSQLLVNRKFQSTLLDKVLPLLNEEEKAFVKRGINSKGAGRYGNDPAYRKSTGFEALVGYLYLTNQERLYNLLMEVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFD8</accession>
    <name>B7IFD8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative hydroxypyruvate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_302</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_302"/>
    <dbReference type="EnsemblBacteria" id="ACJ74802">
        <property type="protein sequence ID" value="ACJ74802"/>
        <property type="gene ID" value="THA_302"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_302"/>
    <dbReference type="eggNOG" id="COG2379">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032279_1_1_0"/>
    <dbReference type="OMA" id="GKAAWRM"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008887">
        <property type="term" value="F:glycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1480.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037035">
        <property type="entry name" value="GK-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038614">
        <property type="entry name" value="GK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007835">
        <property type="entry name" value="MOFRL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025286">
        <property type="entry name" value="MOFRL_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039760">
        <property type="entry name" value="MOFRL_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12227">
        <property type="entry name" value="PTHR12227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13660">
        <property type="entry name" value="DUF4147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05161">
        <property type="entry name" value="MOFRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4147" evidence="2">
        <location>
            <begin position="7"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="MOFRL" evidence="1">
        <location>
            <begin position="296"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="407" mass="44796" checksum="9A9E769478C5A3DB" modified="2009-02-10" version="1">MELKTLAKKIIFDTLEDIQPEKLVKEKLKEIRVDKKVYVLSIGKAAWKMAKAASDVLDIEYGIVITKYGYNFGNIENFDIFEAGHPLPDENSLSATKFAVEKFSKLSKDDILLFLISGGGSSTFELLQDGLSLDDLKDVTTQLLKSGADIKEINTIRSRLSKVKGGRFLNFVKSKVITLVLSDVLENDLRYIASGPTYEACSTFEDAYKIIKKYNLDFNENIINVLKKDVKINKQIDVEHYIIGSIDIACDILEKHARKYNLNTLVLTTRLDCEAKEAGKFIASIAKSSKLKTPYCIIFGGETVVKVKGSGKGGRNQELCYSAALEIENMKDVVIASVGTDGTDGPTDAAGAIVDGKTISKVRKFSNDPNEFLLNNDTYNALKLSGDLLITGPTGTNLNDIGFILKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFE0</accession>
    <name>DNAJ_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperone protein DnaJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaJ</name>
        <name type="ordered locus">THA_304</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DnaJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579485.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFE0"/>
    <dbReference type="SMR" id="B7IFE0"/>
    <dbReference type="STRING" id="484019.THA_304"/>
    <dbReference type="EnsemblBacteria" id="ACJ74804">
        <property type="protein sequence ID" value="ACJ74804"/>
        <property type="gene ID" value="THA_304"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_304"/>
    <dbReference type="eggNOG" id="COG0484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_7_0"/>
    <dbReference type="OMA" id="DLHCTVT"/>
    <dbReference type="OrthoDB" id="1738789at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="SSF49493"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="SSF57938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_1000137733" description="Chaperone protein DnaJ">
        <location>
            <begin position="1"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="162"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="179"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="205"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="219"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="1">
        <location>
            <begin position="149"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01152"/>
        </source>
    </evidence>
    <sequence length="379" mass="42499" checksum="EE33E0ADAF4E8E1C" modified="2009-02-10" version="1">MAKKDYYEILGVSRNASQEEIRQAYKQLIKKWHPDRNHQNRKEAEEKFKEIQEAYEVLSDPEKRAMYDRFGYVGDVPPNAGSGGGFGGFGGFGGFEDIFKDFGDFINNDIFNIFFGDQRTSSRQKQRRAKRGEDINITVDVPFEQVFTGTTIPIEYDRYEVCSHCNGEGVEPGSGWVSCPKCHGTGVVREERRTFLGVIVNQYTCNQCGGTGKIPGETCHVCGGSGRIRKRHRVEVKIPAGVDNGTVIRVQGKGNAGYNGGGYGDLYVNVRITGHIDFERRGNDLIKEIKIDYVEAILGTKVKIKMPDGRVKEVKIPSGVQDGENIYVYGEGVPDMRTGRRGDLILKIKVDIPKRVSRSEKKLLKEIAKLRGKDVDEEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFG8</accession>
    <name>Y332_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative regulatory protein THA_332</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">THA_332</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RemA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103981.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFG8"/>
    <dbReference type="SMR" id="B7IFG8"/>
    <dbReference type="STRING" id="484019.THA_332"/>
    <dbReference type="EnsemblBacteria" id="ACJ74832">
        <property type="protein sequence ID" value="ACJ74832"/>
        <property type="gene ID" value="THA_332"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_332"/>
    <dbReference type="eggNOG" id="COG2052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165326_0_0_0"/>
    <dbReference type="OMA" id="RDRNMLI"/>
    <dbReference type="OrthoDB" id="1786332at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01503">
        <property type="entry name" value="RemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007169">
        <property type="entry name" value="RemA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38449">
        <property type="entry name" value="PTHR38449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04025">
        <property type="entry name" value="DUF370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000146091" description="Putative regulatory protein THA_332">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01503"/>
        </source>
    </evidence>
    <sequence length="94" mass="10556" checksum="0B799ADF8AAA539C" modified="2009-02-10" version="1">MFGLINIGFGNVIAGDRIVAIVNPESAPLKRLKEDAKEEGKLIDATYGRKTRAILISDSNHIILSAIQPETIAQRFMQSFFEIEEQLDKIRRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFH1</accession>
    <name>B7IFH1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-oxoglutarate oxidoreductase, beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579507.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_335"/>
    <dbReference type="EnsemblBacteria" id="ACJ74835">
        <property type="protein sequence ID" value="ACJ74835"/>
        <property type="gene ID" value="THA_335"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_335"/>
    <dbReference type="eggNOG" id="COG1013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048564_2_1_0"/>
    <dbReference type="OMA" id="SAWIASP"/>
    <dbReference type="OrthoDB" id="981516at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="1">
        <location>
            <begin position="51"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="250" mass="27449" checksum="19FDF6795E328C9D" modified="2009-02-10" version="1">MRKVLYKMPESLTGKEFTYCPGCHHGIIHRLIAEVIDELGIREETLVVSPIGCSVFAYQFFNMDGTIAPHGRAPAVATGMKRARPDLYVFTYQGDGDLAAIGTAEIMHAANRGEKITTIFVNNAIYGMTGGQMAPTTLLGMKSTTTPYGRTAENDGYPMHMCEFLKEARGVAYLARTKVNTPQDVEKTKKAIKKAFLAQVKGIGFGMVEVLSTCPTNWGIDPVSANKWLEENMIPEYPLGVFVDKVGDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFI0</accession>
    <name>B7IFI0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Soluble hydrogenase 42 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_344</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104013.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_344"/>
    <dbReference type="EnsemblBacteria" id="ACJ74844">
        <property type="protein sequence ID" value="ACJ74844"/>
        <property type="gene ID" value="THA_344"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_344"/>
    <dbReference type="eggNOG" id="COG0075">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027686_1_1_0"/>
    <dbReference type="OMA" id="KNWLPIM"/>
    <dbReference type="OrthoDB" id="996960at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="6">
        <location>
            <begin position="6"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000524-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="380" mass="42077" checksum="879AEB420AF4F0A3" modified="2009-02-10" version="1">MIKKNYLLAPGPTPVPSEILLEGARETIHHRTPQFVKILEETLNELKYLFQTEHRVYTLLSSGTGALEAAVTNLLNPGDKAIIVEAGKFGERWREIAERFNVNVVSIKLEWGEAVTPEQIKEAIEKHPDAKAVFTTYSETSTGTVIDLEGIAKVTRDTDVVLVTDAVSALLAEPLKMDEWGVDVVVSGSQKGVMLPPGLAFIALNDKAWKLVENCKNSNYYFNLKAYAKKYPDNPWTPGVNLIYMLRKAIQMVKEEGIENVWERHRILADATRAAVKAMGLELFSKRPGNVATAVKVPEGVDGNKLTKIMRDKYGVTIAGGQEHVKGKIFRISTLGYLSIFDTIVGISALEFVLNELGYKVEFGTGVKAAQEVLFKEVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFJ9</accession>
    <name>B7IFJ9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Copper-translocating P-type ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_369</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579528.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_369"/>
    <dbReference type="EnsemblBacteria" id="ACJ74863">
        <property type="protein sequence ID" value="ACJ74863"/>
        <property type="gene ID" value="THA_369"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_369"/>
    <dbReference type="eggNOG" id="COG2217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_0_3_0"/>
    <dbReference type="OMA" id="HWMLPAW"/>
    <dbReference type="OrthoDB" id="237367at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00941">
        <property type="entry name" value="CDATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="668"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="5"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="728" mass="79835" checksum="DBF79A82D7592A62" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFP6</accession>
    <name>B7IFP6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_417</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579564.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_417"/>
    <dbReference type="EnsemblBacteria" id="ACJ74910">
        <property type="protein sequence ID" value="ACJ74910"/>
        <property type="gene ID" value="THA_417"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_417"/>
    <dbReference type="eggNOG" id="COG1507">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097805_2_1_0"/>
    <dbReference type="OMA" id="IAHRCPC"/>
    <dbReference type="OrthoDB" id="1922611at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007511">
        <property type="entry name" value="DUF501"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37163">
        <property type="entry name" value="PTHR37163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04417">
        <property type="entry name" value="DUF501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="176" mass="20569" checksum="633D4ACE11A0AFCF" modified="2009-02-10" version="1">MECFSNKDIRKIVEKQLSINATNFCKVAKFCSYGFPQVIKSLPIKDEKPFPTLNYLVCPYLIKEVSRLEEKGYIKKFQDEVEADKELREKLVNAHMKVIEERDKFFERNDLKKYEQWRSVLKNVGSGGIKDFTKIKCLHLHLADFFAGIENPIGEKVASLLKSLECDDSYCKRFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFP9</accession>
    <name>B7IFP9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_420"/>
    <dbReference type="EnsemblBacteria" id="ACJ74913">
        <property type="protein sequence ID" value="ACJ74913"/>
        <property type="gene ID" value="THA_420"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_420"/>
    <dbReference type="HOGENOM" id="CLU_3067154_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="53" mass="6330" checksum="6576A8D3BF17EA57" modified="2009-02-10" version="1">MLKLFYSPYTLLSPFTKFILSYTPQKIQIDKSQNKIVLVKKSTFEKMRKKTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFR6</accession>
    <name>B7IFR6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_439</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104197.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_439"/>
    <dbReference type="EnsemblBacteria" id="ACJ74930">
        <property type="protein sequence ID" value="ACJ74930"/>
        <property type="gene ID" value="THA_439"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_439"/>
    <dbReference type="eggNOG" id="COG5496">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152014_0_0_0"/>
    <dbReference type="OMA" id="VWNEDIE"/>
    <dbReference type="OrthoDB" id="1682673at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025540">
        <property type="entry name" value="FlK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36934">
        <property type="entry name" value="PTHR36934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="131" mass="14969" checksum="DED0D1372291A007" modified="2009-02-10" version="1">MWRKIEGISKTIDFTPDESYLWDEDDEMRNYHFVSSSSLARVAFKLGADMVKDFLPEDLISVVSEFTVKHYMPVVIGAKLVVGVRVKSVDGNMISFSGLITKDNKKVAEVEFTRVIVSRNYLRRKAVEETT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFS0</accession>
    <name>B7IFS0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_443</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_443"/>
    <dbReference type="EnsemblBacteria" id="ACJ74934">
        <property type="protein sequence ID" value="ACJ74934"/>
        <property type="gene ID" value="THA_443"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_443"/>
    <dbReference type="eggNOG" id="ENOG50331P7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1194426_0_0_0"/>
    <dbReference type="OMA" id="YFEKLNW"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="208" mass="23722" checksum="7737A3AF7D0674AE" modified="2009-02-10" version="1">MLFLASTIFLYVKKAAVKVDVIYIPKPLELNKENSGSEKLPEFSLPSTETMINFEEFDYNKLIAHSIEILEKGTEVSTFVVDKENALKIVNKYKSFLINILSENTYFVVLPIKDVKIPGLISGKSVYTIFLKTGENPEQIFQDMMTLKANGYVSYSMRFKRNNKTFYTLCLGAFPDIDTAKEYFNSLDLEKLRSLVVTYGPYVGKITP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFX0</accession>
    <name>B7IFX0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_493</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104299.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_493"/>
    <dbReference type="EnsemblBacteria" id="ACJ74984">
        <property type="protein sequence ID" value="ACJ74984"/>
        <property type="gene ID" value="THA_493"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_493"/>
    <dbReference type="eggNOG" id="ENOG503331X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160070_0_0_0"/>
    <dbReference type="OMA" id="VKYEHEL"/>
    <dbReference type="OrthoDB" id="1773909at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="117" mass="14178" checksum="06E68A4F995196D2" modified="2009-02-10" version="1">MEKGKKIISFVKYEHEIEPSYREKLANAKRKSDVREVFINTLLELLSSIDPEFNEFSQEDIKFESEYYEFSKKLEEKLEKFKDSDLHAIIERIFEAAKNRYLKIEHDENTDYFNLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFX4</accession>
    <name>B7IFX4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_498</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579616.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_498"/>
    <dbReference type="EnsemblBacteria" id="ACJ74988">
        <property type="protein sequence ID" value="ACJ74988"/>
        <property type="gene ID" value="THA_498"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_498"/>
    <dbReference type="HOGENOM" id="CLU_1288370_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="214" mass="25379" checksum="D816B646508574C4" modified="2009-02-10" version="1">MKLKDISYTDIKHLKWIFSTVTVEDAKNGAIVLGVELYNLFVYKKHYTDFFEIKLFFDNLKGILPRSINSEEACVYISKVLNYFFDYDGELLYTNSNRSLVKILEKYYDEKYSKNLGKLLNDNIDYFIVLFNKSNNGLIENVLMQSNIILDNKVYIDKCANFKKDISEVYVSTDEKMNQNVEEIKRIRDLEDLKNLLPSYYLFFEEEYESILLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG48</accession>
    <name>B7IG48_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribosomal subunit interface protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_575</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100551.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_575"/>
    <dbReference type="EnsemblBacteria" id="ACJ75062">
        <property type="protein sequence ID" value="ACJ75062"/>
        <property type="gene ID" value="THA_575"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_575"/>
    <dbReference type="eggNOG" id="COG1544">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071472_0_2_0"/>
    <dbReference type="OMA" id="VFRNMET"/>
    <dbReference type="OrthoDB" id="1766263at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00552">
        <property type="entry name" value="RaiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036567">
        <property type="entry name" value="RHF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003489">
        <property type="entry name" value="RHF/RaiA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032528">
        <property type="entry name" value="Ribosom_S30AE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038416">
        <property type="entry name" value="Ribosom_S30AE_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16321">
        <property type="entry name" value="Ribosom_S30AE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02482">
        <property type="entry name" value="Ribosomal_S30AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69754">
        <property type="entry name" value="SSF69754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Ribosom_S30AE_C" evidence="2">
        <location>
            <begin position="117"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="176" mass="20705" checksum="B5D59E90A1CB029D" modified="2009-02-10" version="1">MDYRVSAKGFDLTNAVKNYIEKRFQKVDRVLEDEVHLDIKLEKDATSFIGKAMIHYLGKDLVVTETSDDIYNLIDILSDAFEKKLKREREYVRTRNKSNLKGLGEVFKDEMPEEESKEKISSIKRVNLMITSVEEAIAQMEVMNHEFFVFRNMETDEINMLIKGKDGKFILYEFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG97</accession>
    <name>B7IG97_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase, gnat family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579692.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_625"/>
    <dbReference type="EnsemblBacteria" id="ACJ75111">
        <property type="protein sequence ID" value="ACJ75111"/>
        <property type="gene ID" value="THA_625"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_625"/>
    <dbReference type="eggNOG" id="COG0454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118578_0_0_0"/>
    <dbReference type="OMA" id="CYDTTAR"/>
    <dbReference type="OrthoDB" id="2055010at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="154" mass="17259" checksum="39F8B3646B3D4665" modified="2009-02-10" version="1">MSDLIVKLYDLDFSYDLPENILIKRPIGPEKIHVVEWALKNFGKLWASEVDVAFSRNPISVFIAYEKESKDIVGFACYDTTLRGFFGPTGVLKEYRKKGIGKALLLRALQDMLNVGYAYAIIGDAGPVEYYKKTVGAIEIPDSSPGVYKDLLDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGA0</accession>
    <name>RL11_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplK</name>
        <name type="ordered locus">THA_631</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">One or more lysine residues are methylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104446.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IGA0"/>
    <dbReference type="SMR" id="B7IGA0"/>
    <dbReference type="STRING" id="484019.THA_631"/>
    <dbReference type="EnsemblBacteria" id="ACJ75114">
        <property type="protein sequence ID" value="ACJ75114"/>
        <property type="gene ID" value="THA_631"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_631"/>
    <dbReference type="eggNOG" id="COG0080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074237_2_0_0"/>
    <dbReference type="OMA" id="CKQFNAK"/>
    <dbReference type="OrthoDB" id="1702697at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_L11/L12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_L11/L12_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006519">
        <property type="entry name" value="Ribosomal_L11_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_L11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_L11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_L11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_L11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="PTHR11661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="SSF46906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="SSF54747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01632">
        <property type="entry name" value="L11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000195738" description="50S ribosomal protein L11">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <sequence length="141" mass="15258" checksum="C291F8CEA2460B12" modified="2009-02-10" version="1">MAKKVVAQVRLQLEAGKATPAPPVGPALGQRGVNLMEFCKKFNAATADKAGMIIPVIITVYEDRSFTFITKTPPASFLLKKAAKLNSGSQEPKRKMVGKVTRDQIKEIAEIKMKDLNANDIEAAMKIIEGTAKSMGIEVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGA9</accession>
    <name>B7IGA9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="8">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glycyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="8">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">glyS</name>
        <name evidence="9" type="ordered locus">THA_645</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_645"/>
    <dbReference type="EnsemblBacteria" id="ACJ75123">
        <property type="protein sequence ID" value="ACJ75123"/>
        <property type="gene ID" value="THA_645"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_645"/>
    <dbReference type="eggNOG" id="COG0751">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007220_2_2_0"/>
    <dbReference type="OMA" id="LPIPKRM"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00255">
        <property type="entry name" value="Gly_tRNA_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015944">
        <property type="entry name" value="Gly-tRNA-synth_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006194">
        <property type="entry name" value="Gly-tRNA-synth_heterodimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30075">
        <property type="entry name" value="PTHR30075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02092">
        <property type="entry name" value="tRNA_synt_2f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01045">
        <property type="entry name" value="TRNASYNTHGB"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00211">
        <property type="entry name" value="glyS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50861">
        <property type="entry name" value="AA_TRNA_LIGASE_II_GLYAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="637" mass="73877" checksum="2A310587AD8A082D" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGC4</accession>
    <name>B7IGC4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdopterin oxidoreductase, 4Fe-4S cluster-binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_661</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104519.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_661"/>
    <dbReference type="EnsemblBacteria" id="ACJ75138">
        <property type="protein sequence ID" value="ACJ75138"/>
        <property type="gene ID" value="THA_661"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_661"/>
    <dbReference type="eggNOG" id="COG3862">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148086_0_0_0"/>
    <dbReference type="OMA" id="PRGKEWA"/>
    <dbReference type="OrthoDB" id="1751763at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036593">
        <property type="entry name" value="CPE0013-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012460">
        <property type="entry name" value="DUF1667"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39450">
        <property type="entry name" value="PTHR39450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07892">
        <property type="entry name" value="DUF1667"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160148">
        <property type="entry name" value="SSF160148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="119" mass="13219" checksum="FED312282AD9E5EA" modified="2009-02-10" version="1">MIKKLTCISCPIGCELTVYVEGDDIKVEGNRCPRGFEYAKNEVTNPQRMLTISVKVENGVMDLVSARTDRPIPKKMLEEAISYIKGLKVQAPIKRGDIIVKDLLNTGANLIATRTVLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGE4</accession>
    <name>B7IGE4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Negative regulator of genetic competence ClpC/mecB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_686</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579727.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_686"/>
    <dbReference type="EnsemblBacteria" id="ACJ75158">
        <property type="protein sequence ID" value="ACJ75158"/>
        <property type="gene ID" value="THA_686"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_686"/>
    <dbReference type="eggNOG" id="COG0542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005070_4_1_0"/>
    <dbReference type="OMA" id="GPEHILM"/>
    <dbReference type="OrthoDB" id="44062at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clp R" evidence="8">
        <location>
            <begin position="4"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="735" mass="83555" checksum="453A9356B61646E4" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGH5</accession>
    <name>B7IGH5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_719</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579752.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_719"/>
    <dbReference type="EnsemblBacteria" id="ACJ75189">
        <property type="protein sequence ID" value="ACJ75189"/>
        <property type="gene ID" value="THA_719"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_719"/>
    <dbReference type="HOGENOM" id="CLU_1474506_0_0_0"/>
    <dbReference type="OMA" id="DVINIYL"/>
    <dbReference type="OrthoDB" id="1946416at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="183" mass="22220" checksum="98B9C7329F2F226E" modified="2009-02-10" version="1">MEELLCAENVARIITRLKPIDFKYEEQYKNFFDVINIYLTTKDEELARYILDEEFEDLFEIFKKIICKTKFFNFINPTKRVFYKTFDIGERKVKIYLNTHFHVKHRNKVFYYYILPDMREWNEKTLKHFHRLGNDILNSNLPPDQSEFRIVNVIKGKIIKGCNVSYKYHRAISSVVYESFSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGI5</accession>
    <name>B7IGI5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Electron transfer flavoprotein subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_732</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579759.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_732"/>
    <dbReference type="EnsemblBacteria" id="ACJ75199">
        <property type="protein sequence ID" value="ACJ75199"/>
        <property type="gene ID" value="THA_732"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_732"/>
    <dbReference type="eggNOG" id="COG2025">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034178_1_1_0"/>
    <dbReference type="OMA" id="WRPYAEQ"/>
    <dbReference type="OrthoDB" id="1400253at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="PTHR43153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ETF" evidence="5">
        <location>
            <begin position="4"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="323" mass="35758" checksum="C7A0150A3CE89A86" modified="2009-02-10" version="1">MKEIWVYIETFNKKVKKVSLELLTKANELSQKLSAKVVAVYIDKDDLDDTISKYGADKIVHLYNKDLQFYDSQIYTNALYNLIKDDRPHILLIGATSVGRDLAPRLAAKLNTGLTADCTQLDLDEDGLLLQTRPAFGGNIMATIICPEHIPQMATVRPGIFDAKKLEKIPEIEKIEFELEKNCTTKTLEIMKKNSLLKDITESDVIVAGGRGLSKKEGFDLLRDLAKELNGTIAASRAAVEAGWAPQEIQVGQTGKTVKPKIYIACGISGAIQHIAGMKDSKCIIAINKDKNAPIFKIADYAIVGDVYEVVPKLIEKLRVLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGJ3</accession>
    <name>B7IGJ3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_741</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_741"/>
    <dbReference type="EnsemblBacteria" id="ACJ75207">
        <property type="protein sequence ID" value="ACJ75207"/>
        <property type="gene ID" value="THA_741"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_741"/>
    <dbReference type="HOGENOM" id="CLU_3259067_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="42" mass="5355" checksum="6C3C627A5A7C01AA" modified="2009-02-10" version="1">MKNKPVFDLHILEPFKQFKKYKFFENYIKNKYSFKEIIIYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGJ5</accession>
    <name>B7IGJ5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Wzx, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_743</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579768.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_743"/>
    <dbReference type="EnsemblBacteria" id="ACJ75209">
        <property type="protein sequence ID" value="ACJ75209"/>
        <property type="gene ID" value="THA_743"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_743"/>
    <dbReference type="eggNOG" id="COG2244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_7_4_0"/>
    <dbReference type="OMA" id="VWFLDIA"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="434"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="472" mass="53438" checksum="1AE6E4B65902044D" modified="2009-02-10" version="1">MKQYRKLAINTFLFAIGNVGSRSISFLLLPLFTRYMVPSDYGKLDVINTTVSLLVPLLSFQIIEATFRFAADVKETGKGKNVFSSAFLFSFLSFLFSVCLYPLLRNIDIFAEFSLYFYSIFFLTVLNGVVKQFVRGLEKIKLYVISDILYSGVFAGSNIILLVVMKLGIRAYLLSNIVALLSSILFIFFSAGLVKYLSPRIDSKTLKEMLSYSIPLIPNGIMWWMVNASDRYFLSYFIGYEATGMYSVASRFPSLITTLYAVFFQAWQLSAIEEFGKEGYGRFFKNVFGMLSSVMFLGSSILFLLIKPFMRVYVGATYFESWKYTVFLFLGAVFNTFASFYGVNYTASKRTAGAFSTSVIAATTKVAVVLSLIKILRIQAASISTFLAYFVMWIVRIFHTRKIVKVELDVKHIVSSSAIVITQAIFLLMIESTVLLYVLQAVLLTILLWSQREYISKAFRFGRSLLGSKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGJ8</accession>
    <name>B7IGJ8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase, group 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_746</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_746"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75212">
        <property type="protein sequence ID" value="ACJ75212"/>
        <property type="gene ID" value="THA_746"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_746"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_4_0"/>
    <dbReference type="OMA" id="MHILILP"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="101"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="238"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="410" mass="46450" checksum="CEF5426D3F013609" modified="2009-02-10" version="1">MFVSKHIEKRGVVLKILILTNLFPKKENPNSGIFITKRLKEHEKLGVDFTAVSLAFRDKGRLLSLLRSLLRKPSEVPLKELEGVSFKPVFVERGLFDVVVQKFWTMKRALENLTDRFAEQVVQNFPKHTVIHAHGMYLPAPAGVVARKVSEIWNVPYVVTLHGSDVNLNMARRDVRDIYLETLENAARCIFVSRALLEKAKSFGYSGQNATVIPNGYDPDVFKPMDKNTVIKELGIYKEDTQYVGFVGNLIPIKRADKLPEIFRKIAKELPNTRFLIVGNGVLRDKILKEMEGLDVIFAGRVPQVEVAKYMNAMNVMVLPSRNEGWPCVVLEAQACGTCVVGSSNGGIPEAIGFDEYVVEEGENFEERFAKRVVDVLKKGYDRDRLIERAKGFTWEEIVKREISILNKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGJ9</accession>
    <name>B7IGJ9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579772.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_747"/>
    <dbReference type="EnsemblBacteria" id="ACJ75213">
        <property type="protein sequence ID" value="ACJ75213"/>
        <property type="gene ID" value="THA_747"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_747"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1255445_0_0_0"/>
    <dbReference type="OrthoDB" id="842576at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="220" mass="25521" checksum="F36760D571D40A45" modified="2009-02-10" version="1">MDKIIKKLKQIDPFSVFNLIEIHKKYGTTGTFFFLPDNQPKKFGNGYKLHRNIKHLQLLEKEIMAINGSIGIHYDARYLEENRMKNDLDNLKNVFSSIMEAGRAHYLLFDITKSFKILENSGIKIDTTCSYADRVGFRFGTCKPFNPYNFEKNREYGLLEIPLVVMDGTLQSKKYMGLTPKEGLKKIKELINKTIRYKGTLANVTEKVERVKVEKRATPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGL0</accession>
    <name>B7IGL0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA-dihydrouridine synthase</fullName>
            <ecNumber evidence="10">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">nifR</name>
        <name evidence="13" type="ordered locus">THA_759</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 10">Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 5,6-dihydrouridine in tRNA + NAD(+) = a uridine in tRNA + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:54452"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13887"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 5,6-dihydrouridine in tRNA + NADP(+) = a uridine in tRNA + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:23624"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13887"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 10">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the dus family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579782.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_759"/>
    <dbReference type="EnsemblBacteria" id="ACJ75224">
        <property type="protein sequence ID" value="ACJ75224"/>
        <property type="gene ID" value="THA_759"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_759"/>
    <dbReference type="eggNOG" id="COG0042">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013299_0_3_0"/>
    <dbReference type="OMA" id="RPWLFAD"/>
    <dbReference type="OrthoDB" id="1710586at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024036">
        <property type="entry name" value="tRNA-dHydroUridine_Synthase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 10" id="KW-0288">FMN</keyword>
    <keyword evidence="9 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Dus" evidence="12">
        <location>
            <begin position="7"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ75224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="296" mass="33091" checksum="E6EB9B23338557EA" modified="2009-02-10" version="1">MIGKIGLAPMAGVTNWSFRKLCFGFGAEFAYTEMISAESVIRNLKINEYYFPKPEEKDKVAIQIFGSDPFVMAKAASMLENMGAWIDINAGCPVKKVVKKGAGSALLKDLNRLKNIIMQVKSAVSCKVSVKVRIGFEKDEFEKIYDTVVDAGADMIAVHGRTAKQMYSGKATWNIKNKGYIPLYINGDIHSLSDAKRAMEISKADGVLIARASIGKPWVFNDKPPSLSEIKKIILYHIDLLYSEIGERAAQEFRKFVAGYTKNLPNSRQFRAKVMKINDVETLKKAFEEYLTSISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGL6</accession>
    <name>B7IGL6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidoglycan glycosyltransferase</fullName>
            <ecNumber evidence="1">2.4.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_765</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100309.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_765"/>
    <dbReference type="CAZy" id="GT51">
        <property type="family name" value="Glycosyltransferase Family 51"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75230">
        <property type="protein sequence ID" value="ACJ75230"/>
        <property type="gene ID" value="THA_765"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_765"/>
    <dbReference type="eggNOG" id="COG0744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_2_7_0"/>
    <dbReference type="OMA" id="YQLTSMM"/>
    <dbReference type="OrthoDB" id="652304at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transgly" evidence="9">
        <location>
            <begin position="54"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="8">
        <location>
            <begin position="299"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="635" mass="72548" checksum="7DE6E03AF9DC7652" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGN9</accession>
    <name>B7IGN9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxylesterase BioH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_791</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100363.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="SMR" id="B7IGN9"/>
    <dbReference type="STRING" id="484019.THA_791"/>
    <dbReference type="EnsemblBacteria" id="ACJ75253">
        <property type="protein sequence ID" value="ACJ75253"/>
        <property type="gene ID" value="THA_791"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_791"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_13_2_0"/>
    <dbReference type="OMA" id="LLFWGKD"/>
    <dbReference type="OrthoDB" id="1119700at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="22"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="160"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="252" mass="28697" checksum="CF54E162261A7BE2" modified="2009-02-10" version="1">MKKKISDITINYKVIENGTKNILFLHGWGANLNSFLPVAKELKCRSFLIDLPGFGESSLPNSIYSSYDYANLIELFINELNLDNLILVGHSFGGKIASLIASKNPSWLEKVVIISSPGIKTKKKFKTRLKIFTYKLLAKIFYTFGLKKSLEKLRNKFGSQDYKSSKGIMREILKNVINEDISTELKKIKKDTLIIFGKNDDAVPVEVGIKFNQYIENSKLIVYENAGHFPFLENFDSFLYDLKKFIGEINNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGP3</accession>
    <name>B7IGP3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome biogenesis GTPase A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_795</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for a late step of 50S ribosomal subunit assembly. Has GTPase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class YlqF/YawG GTPase family. MTG1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579801.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_795"/>
    <dbReference type="EnsemblBacteria" id="ACJ75257">
        <property type="protein sequence ID" value="ACJ75257"/>
        <property type="gene ID" value="THA_795"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_795"/>
    <dbReference type="eggNOG" id="COG1161">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011106_1_0_0"/>
    <dbReference type="OMA" id="WKLIVIN"/>
    <dbReference type="OrthoDB" id="1083771at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019991">
        <property type="entry name" value="GTP-bd_ribosome_bgen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016478">
        <property type="entry name" value="GTPase_MTG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006230">
        <property type="entry name" value="MG442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03596">
        <property type="entry name" value="GTPase_YlqF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="3">
        <location>
            <begin position="105"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="54"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="113"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006230-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="258" mass="29430" checksum="25144761DA262AE8" modified="2009-02-10" version="1">MWYPGHISKAKRKIKEYLKAVDTIMIILDARAPLATTAFEENIFSNKDVLFVLNKADLSDETYNNLWVKEISKTNPVITFSKKESVEKIKKFIKTYSKEKLKETRILIAGVPNVGKSSIINKVSGRKLAKTGMAPGITRGLQWINLGKVKLLDTPGILYSKLFNKDVTAKLLLIGSIPVESVDKYDIITRAYEIFSNELNIKETFEEFIENFGKKRGYLAKGGLVDFERAKNNFFKAVAEGKFGRMTFDKEVQLWMQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGS2</accession>
    <name>B7IGS2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hydroxylamine reductase</fullName>
            <ecNumber evidence="6">1.7.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Hybrid-cluster protein</fullName>
            <shortName evidence="6">HCP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Prismane protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hcp</name>
        <name evidence="7" type="ordered locus">THA_824</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>A + H2O + NH4(+) = AH2 + H(+) + hydroxylamine</text>
            <dbReference type="Rhea" id="RHEA:22052"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15429"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="1.7.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </cofactor>
        <text evidence="6">Binds 1 hybrid [4Fe-2O-2S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HCP family.</text>
    </comment>
    <dbReference type="EC" id="1.7.99.1" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579818.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_824"/>
    <dbReference type="EnsemblBacteria" id="ACJ75286">
        <property type="protein sequence ID" value="ACJ75286"/>
        <property type="gene ID" value="THA_824"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_824"/>
    <dbReference type="eggNOG" id="COG1151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038344_0_0_0"/>
    <dbReference type="OMA" id="CAYAQGM"/>
    <dbReference type="OrthoDB" id="337713at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050418">
        <property type="term" value="F:hydroxylamine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01914">
        <property type="entry name" value="HCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00069">
        <property type="entry name" value="Hydroxylam_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010048">
        <property type="entry name" value="Hydroxylam_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016100">
        <property type="entry name" value="Prismane_a-bundle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="PTHR30109"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109:SF0">
        <property type="entry name" value="PTHR30109:SF0"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01703">
        <property type="entry name" value="hybrid_clust"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via persulfide group" evidence="6">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Cysteine persulfide" evidence="6">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="433" mass="48231" checksum="EABE166406187AE8" modified="2009-02-10" version="1">MGFNMFCYQCSQAMNGEGCTVTGVCGKSPTVDRLQDNLVYILKGISAYYYHARELGYVDEEIAAYLGKGLYTTLTNVNFDAENIVDLALEAGMMNYKVMQLLKKAHIETYGEPTPTEVETGTKKGHAIIVTGHNLKALEELLKQVEGTDVYVYTHSEMLPAHGYPGLRKYKNLVGNLGKAWFDQRELFDKIPAAILGTSNCVLIPKDSYKDRMFTTSIAKLPGVKHIEGYDYTEVIEKAKDLPKLEEKPGDYKLVTGYSTSVILSLADKIKDYVEKGKIRHFFVVGGCDTPTRKGSYYREFVEKLPKDTVVITLACGKFRINDLDLGDIDGIPRLIDVGQCNDTIVAIEIAEALSKLFNVGINELPLSLVLTWMEQKAVAILWTLLALGVKGIYLGPVLPAWVNEDILKVLQTNYDLKLIGDPEEDIKTILKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGS9</accession>
    <name>B7IGS9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_831</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579821.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_831"/>
    <dbReference type="EnsemblBacteria" id="ACJ75293">
        <property type="protein sequence ID" value="ACJ75293"/>
        <property type="gene ID" value="THA_831"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_831"/>
    <dbReference type="eggNOG" id="COG1611">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107614_0_0_0"/>
    <dbReference type="OMA" id="ANPYVDI"/>
    <dbReference type="OrthoDB" id="1255127at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005268">
        <property type="entry name" value="CHP00725"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041164">
        <property type="entry name" value="LDcluster4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18306">
        <property type="entry name" value="LDcluster4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00725">
        <property type="entry name" value="TIGR00725"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="169" mass="18361" checksum="F62376955F295BE2" modified="2009-02-10" version="1">MKKVAVIGSSGKIDENLEKLCVKLGEELSKNYIVITGGRDGVMEAVSKGVKNNLGTSIGILPYDEKGNEFNTIEISTGLDFSTRSFIILRNADAVISIAGECGTAIEIFGAYAYKKPLILFKGTGGWTDRIQNVLIDGKYLDNRKHSEIYIADSIDKILKILEKILGGS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGT1</accession>
    <name>SYI_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="1">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ileS</name>
        <name type="ordered locus">THA_833</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579823.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IGT1"/>
    <dbReference type="SMR" id="B7IGT1"/>
    <dbReference type="STRING" id="484019.THA_833"/>
    <dbReference type="EnsemblBacteria" id="ACJ75295">
        <property type="protein sequence ID" value="ACJ75295"/>
        <property type="gene ID" value="THA_833"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_833"/>
    <dbReference type="eggNOG" id="COG0060">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_7_0_0"/>
    <dbReference type="OMA" id="HLGTAWN"/>
    <dbReference type="OrthoDB" id="32262at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07960">
        <property type="entry name" value="Anticodon_Ia_Ile_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02002">
        <property type="entry name" value="Ile_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033708">
        <property type="entry name" value="Anticodon_Ile_BEm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023585">
        <property type="entry name" value="Ile-tRNA-ligase_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010663">
        <property type="entry name" value="Znf_FPG/IleRS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06827">
        <property type="entry name" value="zf-FPG_IleRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000189208" description="Isoleucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="590"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="549"/>
        </location>
        <ligand>
            <name>L-isoleucyl-5'-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:178002"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="593"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="878"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="881"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="898"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="901"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02002"/>
        </source>
    </evidence>
    <sequence length="910" mass="105389" checksum="3A1050A32E0C1698" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGU7</accession>
    <name>B7IGU7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579831.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_850"/>
    <dbReference type="EnsemblBacteria" id="ACJ75311">
        <property type="protein sequence ID" value="ACJ75311"/>
        <property type="gene ID" value="THA_850"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_850"/>
    <dbReference type="eggNOG" id="ENOG5033TCG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_419057_0_0_0"/>
    <dbReference type="OMA" id="GENIGIK"/>
    <dbReference type="OrthoDB" id="147764at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="599" mass="71489" checksum="6763B884560D54B4" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGU8</accession>
    <name>B7IGU8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_851</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579832.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_851"/>
    <dbReference type="EnsemblBacteria" id="ACJ75312">
        <property type="protein sequence ID" value="ACJ75312"/>
        <property type="gene ID" value="THA_851"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_851"/>
    <dbReference type="eggNOG" id="ENOG50341A6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_660440_0_0_0"/>
    <dbReference type="OMA" id="LECNISY"/>
    <dbReference type="OrthoDB" id="1578527at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="416" mass="48667" checksum="B8B4530BBFF3F334" modified="2009-02-10" version="1">MKKLILIFLGIGLTLFSNVPIIKINITAPSYIEIINATIYDSIIESGKFKLYLQDELEKLYKELRLQDLLSNKNLILKESKQPIINFDVVIKNLQIKEEKIKKDYIVNNESGDYIRVGEKYIKVQKGIYYSYDVNTSRYIPSKDGYYIKGYDGKFYTFNDFYTKFSHEKIVYKVSGKVYYNLFGYANDSFSFSKEIKKRWYKWDETILKPMKKENSQIEILKYIASVIKSNILENIKNAYKLEGLIYDLKKDRVILNIGKKDGVLPGTVFTIAENKGQIVIKNVENNYSEAEFYYLKKHSKIEIGDQVVEDSKKIFIPISLGIYYTSNGLKISLGLRQRDVYNDKKAFLVVDYLTNTQNYIFTLGYNIFDFYELKTYLSLSSNYSIGGMIKYDLFDLMFNYSLQNKTFNFGGGFSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGV6</accession>
    <name>B7IGV6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75320">
        <property type="protein sequence ID" value="ACJ75320"/>
        <property type="gene ID" value="THA_860"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_860"/>
    <dbReference type="HOGENOM" id="CLU_3158796_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="48" mass="5510" checksum="86D32FA7E25BCDA2" modified="2009-02-10" version="1">MGYKNLFCSIIIKFVPYLAGIEITTDPRPKFSGGWFVPYLAGIEMNYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGY1</accession>
    <name>B7IGY1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579860.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_885"/>
    <dbReference type="EnsemblBacteria" id="ACJ75345">
        <property type="protein sequence ID" value="ACJ75345"/>
        <property type="gene ID" value="THA_885"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_885"/>
    <dbReference type="eggNOG" id="ENOG50348WU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_713578_0_0_0"/>
    <dbReference type="OMA" id="FSWQFTK"/>
    <dbReference type="OrthoDB" id="2155580at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="370"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="394" mass="47824" checksum="0762D1AC60ED7795" modified="2009-02-10" version="1">MSYLIILLIISYFVYSLDKYSKKIFSISVILILSQFTFSYVFYNSEINRKWIFIYDKPKMSWSFLIFFLLFIFGILWGYYFQEKVKFVFSFLPARSLKYSKFKLKLMYYFLAILSILAFSIAISGVNINFLVVSPRLYEQQFGRSTLINYLYFLNVVAIILSVYYRYLFQKRIKYSKIINILLIFISFFHGIKFTIFDTFLYPIIFSYVVNDDLKTIKDFITRYIIVLIFLFIAIVLFFIFVRGNGVAGIYNYFFSSVYNYFYNIETRPFQFVNPLRLFIPREFNLNFKFEIWGIYGYSLNDGYNTYTGFSMLYSIFNIFYPFIYFPYLLLLIKNFYLKKESGLLNSFLLTYLLYCNLMMFFSWQFTKSKYIYYIFVVFIIDIFSRKRNCIKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGY4</accession>
    <name>B7IGY4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Bacterial sugar transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_889</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial sugar transferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579863.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_889"/>
    <dbReference type="EnsemblBacteria" id="ACJ75348">
        <property type="protein sequence ID" value="ACJ75348"/>
        <property type="gene ID" value="THA_889"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_889"/>
    <dbReference type="eggNOG" id="COG2148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024920_0_0_0"/>
    <dbReference type="OMA" id="GPRPEMV"/>
    <dbReference type="OrthoDB" id="1759731at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003362">
        <property type="entry name" value="Bact_transf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02397">
        <property type="entry name" value="Bac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="229"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_transf" evidence="3">
        <location>
            <begin position="227"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="414" mass="48670" checksum="E5C66E935F3C4600" modified="2009-02-10" version="1">MSISRTLFTIDFVGFFIVSFLTTNSVLFSIFSSFFLVISLFAFRVYENIEVKNDHFLRIIVAYFFGIAIILIFNLPFNFHISKFFFISSFLMSITLLPIIHFVLYKFALVKTPVKKYLVIGRKEEFYDILKEIEEKSKGKIKFVDFINPSPAVLKEKVKTADRVLVCDLELEEYVKDDLEILSNTYKVEYLPNLVEKVLKRIPIKVIEKFEEYYKISFRNVSESPAKRLLDIFGSIVGLIVFSPFMLIAAIAILVEDGRPVVFKQERVGKNERLFEFIKLRSLKNTRIDKNSPNKDIEKRALKIGKIIRKTRIDESLQFLLVLKGKMSLVGPRPEMIEYHEMMKGNIPYYTYRLLLKPGLTGWAQLNYKHSSTLEEYKKKTEYDLYYIKNRNIFLDLQIILQTLEAIFWKRGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH11</accession>
    <name>B7IH11_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar kinase, PfkB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_916</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100795.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_916"/>
    <dbReference type="EnsemblBacteria" id="ACJ75375">
        <property type="protein sequence ID" value="ACJ75375"/>
        <property type="gene ID" value="THA_916"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_916"/>
    <dbReference type="eggNOG" id="COG1105">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050013_0_2_0"/>
    <dbReference type="OMA" id="GHVNMAH"/>
    <dbReference type="OrthoDB" id="1323781at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023314">
        <property type="entry name" value="Myo_inos_IolC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017583">
        <property type="entry name" value="Tagatose/fructose_Pkinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000535">
        <property type="entry name" value="1PFK/6PFK/LacC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="5">
        <location>
            <begin position="35"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="317" mass="35745" checksum="604643122A62DBA7" modified="2009-02-10" version="1">MAVLSVCMNPALDREFYINDFKIDKLHRLSFEMSKMSPGGKAINVAIDLATYGVKTIVTGFIGGYIGNIVLTELRKVSDLITTNFVHIDGETRENIAIIDETNHTLTEINSAGPRVPIEDIDHFLRRFSLIVSSADAVVISGSLPQGVPNQIYGDLTKIALKHGKKVFWETKDEIIQESLKISAPCILRPDFRKDKILFGRELVTQEDYIDAGKELIKYGIKMVVLSYKIDYDIVVTQDGVWFLKPLVEIEHSHLLGTGDTYMAAMVYKCFKSDNLVDIAKYGYAAALAKTWYRAKVPPKLEDIEKAFEKFKIERVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH51</accession>
    <name>B7IH51_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Phosphate propanoyltransferase</fullName>
            <ecNumber evidence="3 6">2.3.1.222</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_956</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in 1,2-propanediol (1,2-PD) degradation by catalyzing the conversion of propanoyl-CoA to propanoyl-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>phosphate + propanoyl-CoA = CoA + propanoyl phosphate</text>
            <dbReference type="Rhea" id="RHEA:28046"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:58933"/>
            <dbReference type="EC" id="2.3.1.222"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Polyol metabolism; 1,2-propanediol degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the PduL family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.222" evidence="3 6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100903.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_956"/>
    <dbReference type="EnsemblBacteria" id="ACJ75415">
        <property type="protein sequence ID" value="ACJ75415"/>
        <property type="gene ID" value="THA_956"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_956"/>
    <dbReference type="eggNOG" id="COG4869">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080676_1_0_0"/>
    <dbReference type="OMA" id="EMRERPI"/>
    <dbReference type="OrthoDB" id="1156172at2"/>
    <dbReference type="UniPathway" id="UPA00621"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051144">
        <property type="term" value="P:propanediol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008300">
        <property type="entry name" value="PTAC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39453">
        <property type="entry name" value="PTHR39453"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06130">
        <property type="entry name" value="PTAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010130">
        <property type="entry name" value="PduL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR010130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="196" mass="21669" checksum="A0A55C9E22530A54" modified="2009-02-10" version="1">MKIKEPGIIAGVSNRHVHLSREDVEILFGKDYQLTPIKDLGQPGQFACQETVIIVGPKGAIEKVRVLGPERKETQIEISLTDAFKIGVRPPVRDSGDLDGTPGIVIVGPKGSVIKDKGVIIAKRHIHMHTSDAEKYGVKDKDIVKVLVEKEGRRLIFDDVLIRVSEKYALEFHVDTDEANAALLKTGDLVYIIDEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH88</accession>
    <name>PYRG_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTP synthase</fullName>
            <ecNumber evidence="1">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="1">CTP synthetase</shortName>
            <shortName evidence="1">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrG</name>
        <name type="ordered locus">THA_993</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + NH4(+) + UTP = ADP + CTP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579918.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IH88"/>
    <dbReference type="SMR" id="B7IH88"/>
    <dbReference type="STRING" id="484019.THA_993"/>
    <dbReference type="MEROPS" id="C26.964"/>
    <dbReference type="EnsemblBacteria" id="ACJ75452">
        <property type="protein sequence ID" value="ACJ75452"/>
        <property type="gene ID" value="THA_993"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_993"/>
    <dbReference type="eggNOG" id="COG0504">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_0"/>
    <dbReference type="OMA" id="EFNNAYR"/>
    <dbReference type="OrthoDB" id="783657at2"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="PTHR11550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000164962" description="CTP synthase">
        <location>
            <begin position="1"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="282"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Amidoligase domain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="1">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="499"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="501"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="15"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="145"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="185"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="185"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="370"/>
            <end position="373"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01227"/>
        </source>
    </evidence>
    <sequence length="526" mass="59145" checksum="6F161620C735DEE8" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH97</accession>
    <name>B7IH97_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1002"/>
    <dbReference type="EnsemblBacteria" id="ACJ75461">
        <property type="protein sequence ID" value="ACJ75461"/>
        <property type="gene ID" value="THA_1002"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1002"/>
    <dbReference type="eggNOG" id="ENOG5033JYA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1160659_0_0_0"/>
    <dbReference type="OMA" id="EIERNYM"/>
    <dbReference type="OrthoDB" id="1624067at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="239" mass="28510" checksum="F9F4AEDB2CB4006F" modified="2009-02-10" version="1">MRKLYSFSFLLFVSFIFAISYHALRLTFEYNEDKLQMVRYEEVYYRDGDKIVFVKVPKKVIWVKLGERYYIGEGTTLKLSPPIKDLEDIFYQYLSYHKISDDFDGEITISEKTFNIKAEKRNGFITKIARKFHSVLTEMQYIYLPDNKTFEDVLSEFKFVDKSDFPMKIYNILNLFLWFDAKISGNDLEIFAIDKEGLEVELTLSKEKGTYKIDNFYLTIKKASEETRKEIENEVRDNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHC3</accession>
    <name>B7IHC3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zn-dependent protease with chaperone function</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">THA_1029</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7 8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579944.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1029"/>
    <dbReference type="EnsemblBacteria" id="ACJ75487">
        <property type="protein sequence ID" value="ACJ75487"/>
        <property type="gene ID" value="THA_1029"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1029"/>
    <dbReference type="eggNOG" id="COG0501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025947_1_0_0"/>
    <dbReference type="OMA" id="LPFKIYK"/>
    <dbReference type="OrthoDB" id="1918093at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07343">
        <property type="entry name" value="M48A_Zmpste24p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027057">
        <property type="entry name" value="CAXX_Prtase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032456">
        <property type="entry name" value="Peptidase_M48_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16491">
        <property type="entry name" value="Peptidase_M48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 8 12" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 7 8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48_N" evidence="11">
        <location>
            <begin position="31"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48" evidence="10">
        <location>
            <begin position="203"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ75487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="408" mass="47481" checksum="8A2B06851456EE77" modified="2009-02-10" version="1">MFKFIFLFIVVFEVLWEEILLVLNLKYSTSKNASVPEVLKDKISDGDFERSKRYLKDKTLLESFSQLVRLGVTLMFLLYGFPFLENIVSSFSNSIIIQGLLFFALYGLITFLVNLPFNIYSTFVIEEKYGFNKMNAKTFVVDIIKSFVVSIILFVPLISLLLWILSVDQNWWWKVSIGYILFQVILTLIYPIFIAPIFNKFTPLEDEKLKEEINKLLKKAGFNILSIYVMDASKRTKKQNAALTGIGKSKRIVLYDTILDYSTEEILAIIAHELGHSKRKHIPKLLTIISVLYTVIFYLVNVVYNYILSSNLFGINMSYTAFTYSFIFISSVVYFAIPIINFLQRKFEFEADRYSAELLETPEYLISSLKSLVKENLSNVNPLPLYKVWHYNHPSPEERIKNLLKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHC9</accession>
    <name>B7IHC9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Secretin and TonB N terminus short domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1035</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579948.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1035"/>
    <dbReference type="EnsemblBacteria" id="ACJ75493">
        <property type="protein sequence ID" value="ACJ75493"/>
        <property type="gene ID" value="THA_1035"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1035"/>
    <dbReference type="eggNOG" id="COG4796">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_616665_0_0_0"/>
    <dbReference type="OMA" id="ITTEYFK"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011662">
        <property type="entry name" value="Secretin/TonB_short_N"/>
    </dbReference>
    <dbReference type="SMART" id="SM00965">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="STN" evidence="5">
        <location>
            <begin position="42"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="444" mass="51655" checksum="B2F7313E13758A69" modified="2009-02-10" version="1">MQRNFLIFLSLIVSIISFAINLDFNNKPLIDVLNAIEDETGVKFIILADDIDRFITLSLKDVSLDVALRSVLLPYGLDYERISNDTFLIYSTTQKKTVKLMPKIYTLKHTSPELISNYLSLNGIENYVIDEKLVFYVDSEEEYKRIISDIEKLDEKINGKILIFSVFKLNKYKEVSTLDDRVLINLYNFSQSYKLFSFVEGGYLSYMDVKMPATATSQVIKIAEFTSNEFILRINSEKENKKLELSIGSDVFSTSLKENNQEQNIVLRGKKEIYILRYKILSDVLAEKVKDIEPFDLDNKQTEITTEYFKGRTDLKIQDNKYLLKLSFESIEILGFEFYTNVIDNFSVGIGFESNLFFLTLNDLIKINDLSLQMNLRYDLENFYLRNLVIYKFNFDRFKIGVGIEFSNDSTEVVFHTNVDFSRYEFGFKISNDKSFGVLLGVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHD0</accession>
    <name>B7IHD0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101069.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75494">
        <property type="protein sequence ID" value="ACJ75494"/>
        <property type="gene ID" value="THA_1036"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1036"/>
    <dbReference type="HOGENOM" id="CLU_1844145_0_0_0"/>
    <dbReference type="OMA" id="WSYNENS"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="139" mass="15984" checksum="4CF07E78C077A735" modified="2009-02-10" version="1">MKKFFLLAILLLNIIIIPQTINILWSYNENSVFVIDDLTKKAIVGYMEISSSPGFYMYILPTTNNSKVTVTNVWIGKKSISMNPNYPTKIGYKIFRWGYGMVSGDLILQFNYPDFDLSLNTINVTLDFNFIPMLSLIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHL9</accession>
    <name>B7IHL9_THEAB</name>
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        <submittedName>
            <fullName evidence="7">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
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        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580020.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1130"/>
    <dbReference type="EnsemblBacteria" id="ACJ75583">
        <property type="protein sequence ID" value="ACJ75583"/>
        <property type="gene ID" value="THA_1130"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1130"/>
    <dbReference type="eggNOG" id="COG0392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039146_3_0_0"/>
    <dbReference type="OMA" id="MKFIIYQ"/>
    <dbReference type="OrthoDB" id="1201040at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00374">
        <property type="entry name" value="TIGR00374"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="324" mass="37422" checksum="72E5390EFF74499F" modified="2009-02-10" version="1">MKKKLILNAIIAVVLSLGLIIAIQFFQGDISGLKNIPAYIFVYTLVGYILMYLIGGLSTKIFLKVLGYKVRYIDCFENYFFGNFFSYITPLYVGGQPFQIYHLSKLGVKSEDGTNFISTRLFETFVVNIILDVWIFKYAFKNLNFGNVSKFIILIGFIVQVALTISILIFMFFPQIFRFLIKPISKLFKLDLTKLENWLSNLKESVFNMWNKNAYIVLIDTAFWFVIVCLHAVPFYLMFKHFAHIDISFLRLVGILSLINTVAYFAPTPGAAGGIEGMYQLSLRGIISSSIISRAILLWRLFSYYIPIFLGLVFIWRIKDWTQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHM9</accession>
    <name>B7IHM9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1141</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580029.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1141"/>
    <dbReference type="EnsemblBacteria" id="ACJ75593">
        <property type="protein sequence ID" value="ACJ75593"/>
        <property type="gene ID" value="THA_1141"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1141"/>
    <dbReference type="eggNOG" id="COG0628">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031275_8_3_0"/>
    <dbReference type="OMA" id="GAYVKRY"/>
    <dbReference type="OrthoDB" id="1403084at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="334" mass="38047" checksum="488D2ED6282E23F2" modified="2009-02-10" version="1">MKDKDLKAAFFVGIYALIFLIFCIIFDTILNIFVFTLMSVLIVNLIAKFFKLIKISTKSSIILGLIIYFFIIIYGIILIIPSVAQQFGEFIATINSVFENKAWESYLENNPDLLSGVNDVMNWIRPKLNDLTTSLLNNIAKGTPTFFITIFYSILLTIYILLYSSWLKNTFPNLFPKDIRNDVATFFDKVYSTLSNFVNVVVINAVITAIAFYIVSSFYFKELAAILSFWAGITNLIPIVGVFFEYVPVFLFSLTLGLKGFIIVNIFVIAIHLALFIIFVNVMKMNLNLNPVLMIIAIIIVNQIFGLVGTFFAVPLLIFIVAYWEKFVKPKFET</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHR8</accession>
    <name>B7IHR8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF58 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1182</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580062.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1182"/>
    <dbReference type="EnsemblBacteria" id="ACJ75632">
        <property type="protein sequence ID" value="ACJ75632"/>
        <property type="gene ID" value="THA_1182"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1182"/>
    <dbReference type="eggNOG" id="COG1721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_666904_0_0_0"/>
    <dbReference type="OMA" id="RSYLLMR"/>
    <dbReference type="OrthoDB" id="848990at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="DUF58" evidence="2">
        <location>
            <begin position="173"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="398" mass="46614" checksum="FCA911DF0F32855E" modified="2009-02-10" version="1">MQIENVRLVFLTVIVLFLNFFRINGFTIFLDAFVIIQWVNYSRIVNIFKDIDIDVKLDKDRVFIDEDFNLIISLCGGKVPLHLSVPELGEGKVYLKDVAVLKHSFAKRGKFNIEKFFLKYDAAFFNIIKKVEKKDIQVKVFPNYEEVYFRKEKLLDLVPNVVSKIRLLEDPTSIIGVRKYEKDPIKMINWKISAKLGDIYVKQTEYTSQGRLFLGLFLNLHSQIFSKTAWRPILNKYVEDAILAANSIVKEVSARNIPVKLIADTSEGIKKEFSSDWIDYYEVLSTSYGSLEGFSFEVYNEIEREIIFNDTLLIITMFLSEKDLEKIMRIRERVSRIIILVMPYGFRRYNTKKFKSYLDIPQDIEAIKKNCAILRENDIHVIVYLENMLLQEGIELVN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHV2</accession>
    <name>RS3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsC</name>
        <name type="ordered locus">THA_1221</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a tight complex with proteins S10 and S14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101447.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHV2"/>
    <dbReference type="SMR" id="B7IHV2"/>
    <dbReference type="STRING" id="484019.THA_1221"/>
    <dbReference type="PRIDE" id="B7IHV2"/>
    <dbReference type="EnsemblBacteria" id="ACJ75666">
        <property type="protein sequence ID" value="ACJ75666"/>
        <property type="gene ID" value="THA_1221"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1221"/>
    <dbReference type="eggNOG" id="COG0092">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058591_0_2_0"/>
    <dbReference type="OMA" id="KTNPIGN"/>
    <dbReference type="OrthoDB" id="1132353at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_B">
        <property type="entry name" value="Ribosomal_S3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005704">
        <property type="entry name" value="Ribosomal_S3_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_S3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_S3_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="SSF54821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01009">
        <property type="entry name" value="rpsC_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000141026" description="30S ribosomal protein S3">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="1">
        <location>
            <begin position="38"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <sequence length="209" mass="23839" checksum="441E69E33F6E1F48" modified="2009-02-10" version="1">MGQKVHPRGFRLGITADWQATWFNEKNYKEYLLEDEEIRKVIKNKYAQAGISEIVIERPDSERVIAIIKSARPGIIIGKKGAEITELRQELEKRFNRRFIVNVEEIKNPEVDAQLVAENIASRIEKRASYKVVMKKAIFNAMKKGAKGIKIMVSGRLAGAEIARTEWYLKGRLPLQTIKSIIDYGTARAETKYGTIGIKVWIYKGDADI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHW4</accession>
    <name>RL30_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmD</name>
        <name type="ordered locus">THA_1233</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101469.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHW4"/>
    <dbReference type="SMR" id="B7IHW4"/>
    <dbReference type="STRING" id="484019.THA_1233"/>
    <dbReference type="EnsemblBacteria" id="ACJ75678">
        <property type="protein sequence ID" value="ACJ75678"/>
        <property type="gene ID" value="THA_1233"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1233"/>
    <dbReference type="eggNOG" id="COG1841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131047_2_1_0"/>
    <dbReference type="OMA" id="GKQNQRD"/>
    <dbReference type="OrthoDB" id="2084745at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01658">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01371_B">
        <property type="entry name" value="Ribosomal_L30_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="L30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005996">
        <property type="entry name" value="Ribosomal_L30_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_L30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_L30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15892">
        <property type="entry name" value="PTHR15892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002211">
        <property type="entry name" value="Ribosomal_L30_bac-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="SSF55129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01308">
        <property type="entry name" value="rpmD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000144728" description="50S ribosomal protein L30">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01371"/>
        </source>
    </evidence>
    <sequence length="61" mass="7177" checksum="F9EF288F7B507AEC" modified="2009-02-10" version="1">MKKLKITLVKSPIGYKYDQKDTVKRLGLRKLNSTVIKDDVPQIRGMIRKVRHLVKVEEIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHY2</accession>
    <name>B7IHY2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutamine synthetase</fullName>
            <ecNumber evidence="3">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">glnA1</name>
        <name evidence="5" type="ordered locus">THA_1251</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580089.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1251"/>
    <dbReference type="EnsemblBacteria" id="ACJ75696">
        <property type="protein sequence ID" value="ACJ75696"/>
        <property type="gene ID" value="THA_1251"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1251"/>
    <dbReference type="eggNOG" id="COG0174">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_1_3_0"/>
    <dbReference type="OMA" id="TFYTHPE"/>
    <dbReference type="OrthoDB" id="416474at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_b-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="SSF54368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln-synt_C" evidence="4">
        <location>
            <begin position="102"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="439" mass="50005" checksum="646C50C05784FFBE" modified="2009-02-10" version="1">MGIDEIFKIIEDEKIRFVRLQFSDINGALKNVEIPSADLKRAFEKGIMFDGSSIEGFVRINESDMYLKPDPDTFAILPWTLEGQRSARIICDVYKQDGTPFEGDPRFRLRKVMQEAKDMGFVPHAGPEVEFFLLPRKNNKPSFEFLDEGGYFDLLPVDIAEHLRSEVAVMLEEMGIDVEATHHEVAPSQHEVDFRYSNALSAADAVQTVKLVIKTLAIKNNLYATFMPKPFFGVNGSGMHVHVSLLSTDLQKNMFFDEKSGDISEHMRYFIGGVLKYSKAITAVTNPTVNSYKRLVPGYEAPVNIAWSLANRSALVRVPSARGMATRIEYRSPDPSCNPYLALAVIIKAGLQGIKDKIEPPLPVEKDIYHMKEEEKDNFGIDHLPSNLKEALDEMEKCELIKETLGEHIFNKFLEMKRLEWKDFSIAVTEWERQRYEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHZ0</accession>
    <name>B7IHZ0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Csh crispr-associated protein, Csh2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1259</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580097.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1259"/>
    <dbReference type="EnsemblBacteria" id="ACJ75704">
        <property type="protein sequence ID" value="ACJ75704"/>
        <property type="gene ID" value="THA_1259"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1259"/>
    <dbReference type="eggNOG" id="COG3649">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071770_0_0_0"/>
    <dbReference type="OMA" id="KWGRSLH"/>
    <dbReference type="OrthoDB" id="598606at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006482">
        <property type="entry name" value="Cas7_Csh2/Csh2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013419">
        <property type="entry name" value="CRISPR-assoc_prot_Csh2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05107">
        <property type="entry name" value="Cas_Cas7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02590">
        <property type="entry name" value="cas_Csh2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01595">
        <property type="entry name" value="cas_CT1132"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="302" mass="35042" checksum="03878D161BD89F6C" modified="2009-02-10" version="1">MNEKENSKVNNSEILFIYDAQFCNPNGDPDEENRPRMDSEREINLVSDLRLKRYIRDYLYEKGYDIFVRKVDDKPVTAEKRMGEFNNSKEEEILDKLIDVRLFGATMPVRGNNRAYIGPVQFNWGYSLNKVELLEASITSQFATSENTQQGTIGKDFRVKYSLIAFFGVVSGRRAEKTKLKNEDLKLLDEAMVKAIPLQATRSKIGQYPRLYMRVEYTDSETVLGDFRDLIKLEEKVESIRDIKECALDISELVETLNKNKEKINKIYYFVDKNLELKVNGEIKTLKDALKDLGDGKLEEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMT2</accession>
    <name>C0QMT2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase repeat family IS605</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_2006</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29499">
        <property type="protein sequence ID" value="ACN29499"/>
        <property type="gene ID" value="THA_2006"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2006"/>
    <dbReference type="eggNOG" id="COG0675">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_9_1_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_IS605" evidence="6">
        <location>
            <begin position="28"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="7">
        <location>
            <begin position="155"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="68"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACN29499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="259" mass="29529" checksum="08E1701B9EC7AF7C" modified="2009-05-05" version="1">MHRKFPTEFKIKNVTFERTATNKYYVSIVLEITDTKITNTSDAIIGIDLGIKDTLTLSTGDKYSLPDLSKYEKKLKRLHRQLSRKQKGSKNREKARLRLAKVYEKITNIKNDWIHKVTHKIVNENQVGKIVVEDININGMMKNSKLSKYIHMQSWGKFITILTYKAKNLGIEVIKANRFFPSSQTCSVCGYVNKEVKDLKIRKWVCPICGTEHDRDINAAKNLAKYGLTIPVGREPSEFTPVDSALAAEPEKGLRAITG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICM5</accession>
    <name>RL28_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmB</name>
        <name type="ordered locus">THA_1307</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580138.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ICM5"/>
    <dbReference type="SMR" id="B7ICM5"/>
    <dbReference type="STRING" id="484019.THA_1307"/>
    <dbReference type="EnsemblBacteria" id="ACJ75752">
        <property type="protein sequence ID" value="ACJ75752"/>
        <property type="gene ID" value="THA_1307"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1307"/>
    <dbReference type="eggNOG" id="COG0227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064548_7_0_0"/>
    <dbReference type="OMA" id="RINVCTS"/>
    <dbReference type="OrthoDB" id="2062238at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00373">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026569">
        <property type="entry name" value="Ribo_L28/L24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037147">
        <property type="entry name" value="Ribo_L28/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001383">
        <property type="entry name" value="Ribosomal_L28"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00830">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00009">
        <property type="entry name" value="L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000121700" description="50S ribosomal protein L28">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="69" mass="7838" checksum="FC26276B43CB1B85" modified="2009-02-10" version="1">MAKCQICGKGPASGKNVSHSNRRTNRWFRPNLQKVRVLREDGTVKRMWVCTDCLSAGKIKRYVSKSVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICS7</accession>
    <name>B7ICS7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1359</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580175.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1359"/>
    <dbReference type="EnsemblBacteria" id="ACJ75804">
        <property type="protein sequence ID" value="ACJ75804"/>
        <property type="gene ID" value="THA_1359"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1359"/>
    <dbReference type="eggNOG" id="COG0561">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044146_3_1_0"/>
    <dbReference type="OMA" id="GAWIQDP"/>
    <dbReference type="OrthoDB" id="1374424at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="266" mass="30289" checksum="9CBADBE09547BB4F" modified="2009-02-10" version="1">MVFVFDIDGTLLKSDYTVSQETVDAIKSLERNGHTVIFASGRMLVSVKKFLKNFFGKDFPVIAYNGGMVWIPEKGVVFEKSVDFNSTKKIIEFLRGKSLHRQIYVDDKLYGEEDNEYIKSYARHADVEYFIVEDLVKLSKEKLPTKILSIADEKVLDNVKDELDKMNLNVDIFKSMNIFLDIVPKNVNKAEALKYLIDKLNCRDQKIVVFGDNHNDIGMFKIADFSVAVENAVEELKKEADYIAPSNDEDGVYVALSNHFSDIISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICV9</accession>
    <name>B7ICV9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1392</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580201.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1392"/>
    <dbReference type="EnsemblBacteria" id="ACJ75836">
        <property type="protein sequence ID" value="ACJ75836"/>
        <property type="gene ID" value="THA_1392"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1392"/>
    <dbReference type="eggNOG" id="COG4198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031277_0_0_0"/>
    <dbReference type="OMA" id="LMFLCPM"/>
    <dbReference type="OrthoDB" id="591335at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008323">
        <property type="entry name" value="UCP033563"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36454">
        <property type="entry name" value="PTHR36454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06245">
        <property type="entry name" value="DUF1015"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033563">
        <property type="entry name" value="UCP033563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="412" mass="47514" checksum="834BFE8B50E329D6" modified="2009-02-10" version="1">MVVRPFRGLRPRKDMIDKVAAKPYDVVSTEEARKEAERNPYTFYKVTRPEINFDGEVDTHSDEVILKGKEVLDNFQKEGVMILEDKPAIYVYKEEWKGYSQIGIYATFSTQEYREGKIKKHELTRKDKEDERARHVKLMQAHTGPVFLMYRSRPEIDQLIIKHTTKEPEYDYTDESGIHHTLWVVKDENEIKEIVDAFKSVDAFYIADGHHRAAAAARAAEELAKENPNHTGEEEYNFFLAVLFPHSQLHILDYNRVVKDLNGNSKEEFLKKISAKFDIEDAPNSPFKPEKKHEFGMYLDGKWYKLSAKEGTFDKNDVVSSLDVAILQNNLLNPILGIENPRTDKRINFVGGIKGVEELQRLVDSGEYTVAFALYPTSIEELLQVADEGKIMPPKSTWFEPKLKSGIIVHLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICW7</accession>
    <name>B7ICW7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Spermidine/putrescine ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1400</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580205.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1400"/>
    <dbReference type="EnsemblBacteria" id="ACJ75844">
        <property type="protein sequence ID" value="ACJ75844"/>
        <property type="gene ID" value="THA_1400"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1400"/>
    <dbReference type="eggNOG" id="COG1176">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_18_3_0"/>
    <dbReference type="OMA" id="RIYAWMI"/>
    <dbReference type="OrthoDB" id="1488093at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="57"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="271" mass="30298" checksum="C89AD0407FF029E1" modified="2009-02-10" version="1">MKYVSGYSIWLVLFFIVPVLIVLSFSFMEKSTYGGVEFSFSLEGYKSLFSIGYLKILWRSIWISLVATLLTILLALPVAYYIANSKIKDLLLLLVVIPFWTNSLIRIYAWIFVLGNNGIINQLLRKIGLVNDYIQFLYNPYAVILVIVYTYLPYAIIPLFASIDRLEKSILEAAFDLGCNKTKAFFKVLIPNIKSGLITATLFVFIPALGSYAIPDLVGGVNSKMIGNEIVRQLTVAKNWPAASAISSILTLATLIGVLLFVKSTQKEVHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICX8</accession>
    <name>B7ICX8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1411</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101816.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1411"/>
    <dbReference type="EnsemblBacteria" id="ACJ75855">
        <property type="protein sequence ID" value="ACJ75855"/>
        <property type="gene ID" value="THA_1411"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1411"/>
    <dbReference type="eggNOG" id="COG1480">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015767_4_1_0"/>
    <dbReference type="OMA" id="PFYFKEN"/>
    <dbReference type="OrthoDB" id="1124669at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011621">
        <property type="entry name" value="Metal-dep_PHydrolase_7TM_intra"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07698">
        <property type="entry name" value="7TM-7TMR_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="HDc" evidence="2">
        <location>
            <begin position="233"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="459" mass="53022" checksum="E4B1E85EE330983F" modified="2009-02-10" version="1">MKKIPLKNFDGVIVSFITVALLNFFDFNIIKAIYELIFLIIIWYLVIEKVFEEVFYFKLHKIYKNTFYFLFIFGVFITYISIKRFDLTLSPFLISSLLLTLLTSYIVGFSAGILQSILIAFHINDFYSAIYMISQILLMNYLIRNINRRIEIAKSAVFTSLLSFALLLDQIPLKPLYLNIYDISYGVLNPFISTIIILGTLPYIEYFTRIYSNIGLSELANMNHPLLKRLSLQAPGTYYHSIMVATLAEAAAERIGANSTFARVASYFHDIGKMIRPYFFVENIPDNQENPHDSISPFLSHLILEDHVKSGIELARKYRLPLTIEFIIPQHHGTRVQKYFYHKAKELEENVSEDSFRYPGPKPQFKEAGIIMLADSVEAALKSLNSSNYQRIKDLVENIVSSIYNERQLDESGLNLKELELIIDEFIKVIVNITKARIEYPKEEIKKVVQANGDNKQTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICY5</accession>
    <name>B7ICY5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Propionyl-CoA carboxylase, beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1418</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580214.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1418"/>
    <dbReference type="EnsemblBacteria" id="ACJ75862">
        <property type="protein sequence ID" value="ACJ75862"/>
        <property type="gene ID" value="THA_1418"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1418"/>
    <dbReference type="eggNOG" id="COG4799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018822_6_0_0"/>
    <dbReference type="OMA" id="FICGPEV"/>
    <dbReference type="OrthoDB" id="886663at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="2">
        <location>
            <begin position="263"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="513" mass="55911" checksum="74E4EAFC5DE9783E" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICY6</accession>
    <name>B7ICY6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methylmalonyl-CoA epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1419"/>
    <dbReference type="EnsemblBacteria" id="ACJ75863">
        <property type="protein sequence ID" value="ACJ75863"/>
        <property type="gene ID" value="THA_1419"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1419"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2774563_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="5"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="69" mass="8041" checksum="606156A823220390" modified="2009-02-10" version="1">MKSNKIDHIGIAVKNAKERLKLYKDFLGLEVSGEEELPERGLKVYFVKIGETRFELLEPLNENSEIHHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID16</accession>
    <name>B7ID16_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Respons regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101889.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1450"/>
    <dbReference type="EnsemblBacteria" id="ACJ75893">
        <property type="protein sequence ID" value="ACJ75893"/>
        <property type="gene ID" value="THA_1450"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1450"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_628413_0_0_0"/>
    <dbReference type="OMA" id="ININEFY"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="425" mass="49687" checksum="A264F88BAEBFA394" modified="2009-02-10" version="1">MPYKILIVDDSYVWRSFLSKHLTNHGYTVEEASDGIDGINKFFDFLPDVVIVDYIMPNLNGIQFSRFLRSFSACKNVGIILLTASNESINEFWAKKSGANLFLNKSIEQEELLKKIDEFLKGTFSIDWSREVYKIHAQPFGELIDILDESLKYSTILSEITNLLVYLNDEELLFSKLHEFFAELFYFSNLYFLILTPSRLRIYNFSDNEKATIKSITEFLYSKIPFLLPFSKISSNYEGEKQLDENNLLLPLDLKGNLLGFLIFENPKNEEQIIYNFNLLKDALSNLFVILNDYYYLFNNITYDDNTKTLVLPIFRSKIQQSIKFAKRNNINFKVFTVEIKNIPHLLKNKGTKYVINLLKKIAEKLNSTFPDATSRITQTKFACISFEDINLDKLLNFEEININEFYWDNKDILEILDLIYRGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID35</accession>
    <name>B7ID35_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PrcB_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580245.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1469"/>
    <dbReference type="EnsemblBacteria" id="ACJ75912">
        <property type="protein sequence ID" value="ACJ75912"/>
        <property type="gene ID" value="THA_1469"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1469"/>
    <dbReference type="eggNOG" id="ENOG50338HY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1102368_0_0_0"/>
    <dbReference type="OMA" id="VTQAFTI"/>
    <dbReference type="OrthoDB" id="1084416at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025748">
        <property type="entry name" value="PrcB_C_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14343">
        <property type="entry name" value="PrcB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PrcB_C" evidence="1">
        <location>
            <begin position="167"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="252" mass="29231" checksum="0C7D6E347EE9E722" modified="2009-02-10" version="1">MKKLFSLFLTIFSIIILSNSVFIYPVDKELPDNLFQYEGTKSNMVYYLKLFENDQEKGYIIKGWYFTPKSAIETTKILIRFNNEEHNYTIKTLRKGNYSIIPSYLIICPKNATVIIGNYEIDKKQITKNVEIGEIMTPQFREMGIYVFTIENKIVLKDTFSTNDDIFIVVSMGLKKTGGYSLELENYEIKENQILINLVLNEPKKGEMVTQAFTIPTYKLNIGKLEKGNYVIKVNVMSVNNVLKFSKEIEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID37</accession>
    <name>B7ID37_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit PurL</fullName>
            <shortName evidence="5">FGAM synthase</shortName>
            <ecNumber evidence="5">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Formylglycinamide ribonucleotide amidotransferase subunit II</fullName>
            <shortName evidence="5">FGAR amidotransferase II</shortName>
            <shortName evidence="5">FGAR-AT II</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glutamine amidotransferase PurL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 10" type="primary">purL</name>
        <name evidence="10" type="ordered locus">THA_1471</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FGAMS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580247.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1471"/>
    <dbReference type="EnsemblBacteria" id="ACJ75914">
        <property type="protein sequence ID" value="ACJ75914"/>
        <property type="gene ID" value="THA_1471"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1471"/>
    <dbReference type="eggNOG" id="COG0046">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003100_0_1_0"/>
    <dbReference type="OMA" id="EHCGYSH"/>
    <dbReference type="OrthoDB" id="26038at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031652">
        <property type="entry name" value="PurL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038411">
        <property type="entry name" value="PurL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PTHR43555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16904">
        <property type="entry name" value="PurL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01736">
        <property type="entry name" value="FGAM_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FGAR-AT_linker" evidence="9">
        <location>
            <begin position="3"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS" evidence="6">
        <location>
            <begin position="65"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="7">
        <location>
            <begin position="169"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS" evidence="6">
        <location>
            <begin position="413"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="PurL_C" evidence="8">
        <location>
            <begin position="508"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="70"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="279"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="478"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="597" mass="65176" checksum="9D28AADE20BFC6F6" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID44</accession>
    <name>B7ID44_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">L-lactate dehydrogenase</fullName>
            <shortName evidence="6">L-LDH</shortName>
            <ecNumber evidence="4 6">1.1.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ldh</name>
        <name evidence="12" type="ordered locus">THA_1478</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of lactate to pyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>(S)-lactate + NAD(+) = H(+) + NADH + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:23444"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.27"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="6">Allosterically activated by fructose 1,6-bisphosphate (FBP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the LDH/MDH superfamily. LDH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.27" evidence="4 6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101959.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="SMR" id="B7ID44"/>
    <dbReference type="STRING" id="484019.THA_1478"/>
    <dbReference type="EnsemblBacteria" id="ACJ75921">
        <property type="protein sequence ID" value="ACJ75921"/>
        <property type="gene ID" value="THA_1478"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1478"/>
    <dbReference type="eggNOG" id="COG0039">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045401_1_1_0"/>
    <dbReference type="OMA" id="CYIIVLT"/>
    <dbReference type="OrthoDB" id="870724at2"/>
    <dbReference type="UniPathway" id="UPA00554">
        <property type="reaction ID" value="UER00611"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00488">
        <property type="entry name" value="Lactate_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011304">
        <property type="entry name" value="L-lactate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018177">
        <property type="entry name" value="L-lactate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01771">
        <property type="entry name" value="L-LDH-NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00064">
        <property type="entry name" value="L_LDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ldh_1_N" evidence="10">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Ldh_1_C" evidence="11">
        <location>
            <begin position="142"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="117"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="145"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6 7">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="76"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="115"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine" evidence="6">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ75921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="303" mass="33788" checksum="5C5FF575CEA500E8" modified="2009-02-10" version="1">MKISIVGAGRVGTSIAYSLLHRKIANEIVIIDKNYEKAYGEALDLYHGTSLLKRCNIYAGNFQDLKNSDIVIITAGAAQKVGETRLDLTKRNYEIIKEISKEIKKCANESIIINVTNPVDVLTYFLWKELDFPKNKVIGTGTILDTARFRALVSKQCGVSPASVHAYIVGEHGDSELMVWSSATIGGVNIKDFCKVCNRKNCRNLRDIFEETKNAAYTIIEKKGATNFAIGVVTAQLVESIFKDEKKVLTPSTYIDGIFIGFPAIVGKNGIERIIPIEMDEFEISLFENSKNIIRKQIEEIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID75</accession>
    <name>B7ID75_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102034.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1511"/>
    <dbReference type="EnsemblBacteria" id="ACJ75952">
        <property type="protein sequence ID" value="ACJ75952"/>
        <property type="gene ID" value="THA_1511"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1511"/>
    <dbReference type="eggNOG" id="COG1078">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026821_3_2_0"/>
    <dbReference type="OMA" id="DGHDEYR"/>
    <dbReference type="OrthoDB" id="370035at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="52"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="502" mass="58863" checksum="0BFA305105C1605B" modified="2009-02-10" version="1">MYYKVSRDPIHAEIFMYPLEIIASDTKAMQRLRYLSQLVGSEYVYPCATHTRFAHSLGVMHISGMYASHLFGDDPAKVRILRLAGLLHDIGHGPFSHQFDEVVYKNMGLEDGHDEYREKILLEYMPKAMYKVFERAPEKLKNAVLEDLNMVLGEVDDLEDDFRKVMEKVVETYKGENEGTIEFAVIQGPLGADRMDFVLRDSYFAGTRGFGTGSLDRLVRNSMVVEKDGKKFLAYNIKVIDEIYTTLFGRFMMYKNVYFHKTSRAADLMIQDLLELCYRPLKLKERVENLETFMELTDQRIINEVEIYFNQIVEEYNISSFEDTKEKILNFEIDLQPVELDIVMAYEIVERLKNRDFWKTILETPFSVEGIDPSAASQGFAMDILQKIRLRLEKAVEISDESDREELLKILSNFDEIFKVDTPYKLSLVHPDEFLKSNIYIYDSWKEHVLSFDEYVREYPAYKLMTSNLIQIVRFYVTKDVRNLLEKYNIIPKVDTSLTTRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID77</accession>
    <name>B7ID77_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102039.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1513"/>
    <dbReference type="EnsemblBacteria" id="ACJ75954">
        <property type="protein sequence ID" value="ACJ75954"/>
        <property type="gene ID" value="THA_1513"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1513"/>
    <dbReference type="eggNOG" id="COG1032">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021572_4_3_0"/>
    <dbReference type="OMA" id="YDFWLKP"/>
    <dbReference type="OrthoDB" id="973846at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="199"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="451" mass="52386" checksum="421042E4CFB3517F" modified="2009-02-10" version="1">MKILLVNPWIEDFAAYDFWLKPIGLLYIASYLKKLGIEIHFIDLMNRHDKSLQKFTKVPKDKFYGTGKFPFVEIKKPDVLAFVPRKYKRYGAPEQYFYDKLKEVGNVDAIFVTSTLTYWYPGYWDTINFLRGYYGNKVPIFFGGFYVRNLPQHARKTNAIIFPFSDLNRIPKMLEDLTGKSVKRIGIDWFMELSPLYDVYENIGYLVFLTTLGCPFKCSYCVAHKLWDGIKFRDPERVVSDIERYVEKFKVKDVVFFDDAFLVNSNKHAKVILKKLLEKNFSKDIRFHLPNGIHARLIDEELAFLMKELNFKTIKLGYETSGELQLKTGGKVVDKDLVNAARILRNAGFTEEEISAYIMVNIPGQKVEDVINAMKICKSEGISFSINEFTPIVGTEDWLKLVSEGKLSGKEDPVLLNNTVLPFWWKHGMDEQTVQNLKMLAREIKEGDCYD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID86</accession>
    <name>GREA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription elongation factor GreA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Transcript cleavage factor GreA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">greA</name>
        <name type="ordered locus">THA_1522</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GreA/GreB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102051.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ID86"/>
    <dbReference type="SMR" id="B7ID86"/>
    <dbReference type="STRING" id="484019.THA_1522"/>
    <dbReference type="PRIDE" id="B7ID86"/>
    <dbReference type="EnsemblBacteria" id="ACJ75963">
        <property type="protein sequence ID" value="ACJ75963"/>
        <property type="gene ID" value="THA_1522"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1522"/>
    <dbReference type="eggNOG" id="COG0782">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101379_2_1_0"/>
    <dbReference type="OMA" id="TWLTQEA"/>
    <dbReference type="OrthoDB" id="1536415at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00105">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036953">
        <property type="entry name" value="GreA/GreB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018151">
        <property type="entry name" value="TF_GreA/GreB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006359">
        <property type="entry name" value="Tscrpt_elong_fac_GreA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028624">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001437">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023459">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022691">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036805">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30437">
        <property type="entry name" value="PTHR30437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01272">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03449">
        <property type="entry name" value="GreA_GreB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006092">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46557">
        <property type="entry name" value="SSF46557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01462">
        <property type="entry name" value="greA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00829">
        <property type="entry name" value="GREAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_1000117407" description="Transcription elongation factor GreA">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00105"/>
        </source>
    </evidence>
    <sequence length="156" mass="17702" checksum="2FB26F64D2D6FE58" modified="2009-02-10" version="1">MKKESIYLTKEGYEKLKAELDQLKQKLMFEIAQRIKEARELGDLSENSEYQEAKNEQGRIAARINELENMLSKAEVIEGLDTNVINIGNWVLIKNLDTGEEKTIQIVTPHEADVFNNKISFESPIGRVLVGKKVGEVVKIKAPKGVFKYQILGIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDB3</accession>
    <name>B7IDB3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1551</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580273.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1551"/>
    <dbReference type="EnsemblBacteria" id="ACJ75990">
        <property type="protein sequence ID" value="ACJ75990"/>
        <property type="gene ID" value="THA_1551"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1551"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_12_2_0"/>
    <dbReference type="OMA" id="ETNSYFA"/>
    <dbReference type="OrthoDB" id="2089619at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="184" mass="21160" checksum="03F1408C685C7C59" modified="2009-02-10" version="1">MARKNTKILILEAAKKAFSEKGYDAVSIDEIAQLAGVQKSLIYYYFPSKEALFEEVWIKSIEELENELFGVEESDSSYVARIKNFLKRYIDFLTSKKAISDLLRREKTKVIDQENWKNAREKYESFLGKIQSLIVEGKKKKIINEEVDPKAATDMIASVDSLPRKGLLKSVENMLIKILLKDNT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDB4</accession>
    <name>RL31_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmE</name>
        <name type="ordered locus">THA_1552</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL31 family. Type A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102224.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDB4"/>
    <dbReference type="STRING" id="484019.THA_1552"/>
    <dbReference type="EnsemblBacteria" id="ACJ75991">
        <property type="protein sequence ID" value="ACJ75991"/>
        <property type="gene ID" value="THA_1552"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1552"/>
    <dbReference type="eggNOG" id="COG0254">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114306_4_3_0"/>
    <dbReference type="OMA" id="WYPDAKV"/>
    <dbReference type="OrthoDB" id="2014569at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00501">
        <property type="entry name" value="Ribosomal_L31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002150">
        <property type="entry name" value="Ribosomal_L31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027491">
        <property type="entry name" value="Ribosomal_L31_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042105">
        <property type="entry name" value="Ribosomal_L31_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33280">
        <property type="entry name" value="PTHR33280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01197">
        <property type="entry name" value="Ribosomal_L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01249">
        <property type="entry name" value="RIBOSOMALL31"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00105">
        <property type="entry name" value="L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000126753" description="50S ribosomal protein L31">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00501"/>
        </source>
    </evidence>
    <sequence length="69" mass="7723" checksum="A751286C62ED651A" modified="2009-02-10" version="1">MKKGIHPEMKLITVKCACGAEHKFYSAKENVRIDVCSSCHPFYKGAGAAGMIVDTEGRIEKFKKKYNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDB6</accession>
    <name>B7IDB6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADP-reducing hydrogenase, subunit c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1554</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102229.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1554"/>
    <dbReference type="EnsemblBacteria" id="ACJ75993">
        <property type="protein sequence ID" value="ACJ75993"/>
        <property type="gene ID" value="THA_1554"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1554"/>
    <dbReference type="eggNOG" id="COG1894">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014881_3_2_0"/>
    <dbReference type="OMA" id="CKQCTYC"/>
    <dbReference type="OrthoDB" id="688908at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="SSF140490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="SSF142019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00644">
        <property type="entry name" value="COMPLEX1_51K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH_4Fe-4S" evidence="6">
        <location>
            <begin position="428"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="537" mass="58888" checksum="385D53FA06AE5307" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDE7</accession>
    <name>B7IDE7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rubredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1586</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="6 7">Binds 1 Fe(3+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the rubredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1586"/>
    <dbReference type="EnsemblBacteria" id="ACJ76024">
        <property type="protein sequence ID" value="ACJ76024"/>
        <property type="gene ID" value="THA_1586"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1586"/>
    <dbReference type="eggNOG" id="COG1773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128747_3_3_0"/>
    <dbReference type="OMA" id="APKDMFE"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024922">
        <property type="entry name" value="Rubredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018527">
        <property type="entry name" value="Rubredoxin_Fe_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000071">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00202">
        <property type="entry name" value="RUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000071-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="52" mass="5883" checksum="37E66A1D9323567E" modified="2009-02-10" version="1">MKRYVCTVCGYVYDPEVGDPDNGIEPGTPFEELPEDWVCPVCGVPKDQFEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDM3</accession>
    <name>B7IDM3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative FAD-binding subunit of oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102472.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1662"/>
    <dbReference type="EnsemblBacteria" id="ACJ76100">
        <property type="protein sequence ID" value="ACJ76100"/>
        <property type="gene ID" value="THA_1662"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1662"/>
    <dbReference type="eggNOG" id="COG1319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073026_0_0_0"/>
    <dbReference type="OMA" id="GGCAFLK"/>
    <dbReference type="OrthoDB" id="1017413at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03450">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="SSF55447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="262" mass="29489" checksum="4CEA1B740246A881" modified="2009-02-10" version="1">MIKFKEYVKPKTLEEAYQILTEKNSRVIGGAAFLKLSNQEFDVAIDLIDTNLDFINDYPEKVEVGSMVTISAFEEDPVLSTLYNGFLKNVAEKILSYQMRNIVTVGGTIFPKYGFSDLITALLVLNTNVELYKNGKMPLEKFLETKIVKDILSKIEIMKENLKLAFEYVRNSEYDFSLLNVAVSNNEGKFKIAVGARPGIAQLAKNAAEFLSNNAITKENIEKAAEIAAEELKFSDDLRATAEYRKKVCKALVKRALEEVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDN2</accession>
    <name>B7IDN2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Band 7/Mec-2 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1671</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102489.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1671"/>
    <dbReference type="EnsemblBacteria" id="ACJ76109">
        <property type="protein sequence ID" value="ACJ76109"/>
        <property type="gene ID" value="THA_1671"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1671"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024949_2_2_0"/>
    <dbReference type="OMA" id="TQIRAEM"/>
    <dbReference type="OrthoDB" id="1213601at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018080">
        <property type="entry name" value="Band_7/stomatin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_HflKC_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01270">
        <property type="entry name" value="BAND_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHB" evidence="2">
        <location>
            <begin position="19"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="305" mass="34323" checksum="7645143B4F458171" modified="2009-02-10" version="1">MLYFLFLILIFFLFLVAASGIRIVRPYERGLVERLGKFRKEVKAGIHFIIPFFDRMIKVDLREHVIDVPPQEVITKDNVVVTVDAVIYYEITDAYKAVYNVSNFEFATIKLAQTNLRNVIGELELDQTLTSREKINTKLRTVLDEATDKWGIRITRVEIKKIDPPKDIMEAMSKQMKAERTKRAAILEAEGIRQSEILKAEGQKQAAILKAEGEAEAIKKVAEANKYKLIAEAQGQGEAIMLVFKSIHEGNPTNDVIAVRYLETLKEMANGNATKIFLPMELSGILGSIGAISEMFKSGGEKDNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDQ0</accession>
    <name>B7IDQ0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76127">
        <property type="protein sequence ID" value="ACJ76127"/>
        <property type="gene ID" value="THA_1690"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1690"/>
    <dbReference type="HOGENOM" id="CLU_3067158_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="53" mass="5781" checksum="658066637CEE928F" modified="2009-02-10" version="1">MDSAVSSGRLNNKMMLANTINGRIYFDFSLNGTNMKAEIKGMAINKAVIFSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDQ4</accession>
    <name>B7IDQ4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Monovalent cation/proton antiporter, MnhG/PhaG subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580354.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1694"/>
    <dbReference type="EnsemblBacteria" id="ACJ76131">
        <property type="protein sequence ID" value="ACJ76131"/>
        <property type="gene ID" value="THA_1694"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1694"/>
    <dbReference type="eggNOG" id="COG1320">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121334_0_0_0"/>
    <dbReference type="OMA" id="TNPISAH"/>
    <dbReference type="OrthoDB" id="2051355at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005451">
        <property type="term" value="F:monovalent cation:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098662">
        <property type="term" value="P:inorganic cation transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005133">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34703">
        <property type="entry name" value="PTHR34703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03334">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01300">
        <property type="entry name" value="CPA3_mnhG_phaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="106" mass="11376" checksum="3C42D64D93462958" modified="2009-02-10" version="1">MSILGYILITIGAFFYFLGGLGIFRMPDVYNRLQAGTKATTLGSFSVILGVGLVNLDYLPKALVIIAFIALTNPVGSSVLARAAYLKGIKPTEKTKVDEYKGGDEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDQ7</accession>
    <name>REX_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Redox-sensing transcriptional repressor Rex</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rex</name>
        <name type="ordered locus">THA_1697</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Modulates transcription in response to changes in cellular NADH/NAD(+) redox state.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulatory Rex family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580357.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDQ7"/>
    <dbReference type="SMR" id="B7IDQ7"/>
    <dbReference type="STRING" id="484019.THA_1697"/>
    <dbReference type="EnsemblBacteria" id="ACJ76134">
        <property type="protein sequence ID" value="ACJ76134"/>
        <property type="gene ID" value="THA_1697"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1697"/>
    <dbReference type="eggNOG" id="COG2344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061534_1_0_0"/>
    <dbReference type="OMA" id="HEQRKAG"/>
    <dbReference type="OrthoDB" id="1872374at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051775">
        <property type="term" value="P:response to redox state"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01131">
        <property type="entry name" value="Rex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009718">
        <property type="entry name" value="Rex_DNA-bd_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022876">
        <property type="entry name" value="Tscrpt_rep_Rex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35786">
        <property type="entry name" value="PTHR35786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06971">
        <property type="entry name" value="Put_DNA-bind_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_1000137337" description="Redox-sensing transcriptional repressor Rex">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="15"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="89"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01131"/>
        </source>
    </evidence>
    <sequence length="207" mass="23579" checksum="EFBBE246FC0030C3" modified="2009-02-10" version="1">MEKKIPRPVIKRLGLYYRCLNRLYEEGIEYVASKDIAERLGIKSSQVRKDLSYFGEFGKRGVGYNTYELMERLEDIIGVNKYWNVIVIGAGNIGSAIANYEGLRKEKFNVIGIFDADRSKVGRKIGRLIVKHFSEIDDFFKKNIVEIAVLAVPENAAQMVVDKLEELGIKGIVNFAPVKLRTNIPVEDVDITLSFKSLSFKIERNIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDT3</accession>
    <name>B7IDT3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase</fullName>
            <ecNumber evidence="2">2.5.1.145</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lgt</name>
        <name evidence="3" type="ordered locus">THA_1724</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the transfer of the diacylglyceryl group from phosphatidylglycerol to the sulfhydryl group of the N-terminal cysteine of a prolipoprotein, the first step in the formation of mature lipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + L-cysteinyl-[prolipoprotein] = H(+) + S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[prolipoprotein] + sn-glycerol 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:56712"/>
            <dbReference type="Rhea" id="RHEA-COMP:14679"/>
            <dbReference type="Rhea" id="RHEA-COMP:14680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
            <dbReference type="ChEBI" id="CHEBI:140658"/>
            <dbReference type="EC" id="2.5.1.145"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; lipoprotein biosynthesis (diacylglyceryl transfer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Lgt family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.145" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102594.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1724"/>
    <dbReference type="EnsemblBacteria" id="ACJ76160">
        <property type="protein sequence ID" value="ACJ76160"/>
        <property type="gene ID" value="THA_1724"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1724"/>
    <dbReference type="eggNOG" id="COG0682">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013386_1_2_0"/>
    <dbReference type="OMA" id="TDVPWAI"/>
    <dbReference type="OrthoDB" id="435911at2"/>
    <dbReference type="UniPathway" id="UPA00664"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008961">
        <property type="term" value="F:phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01147">
        <property type="entry name" value="Lgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001640">
        <property type="entry name" value="Lgt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30589">
        <property type="entry name" value="PTHR30589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01790">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00544">
        <property type="entry name" value="lgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01311">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol)</name>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="293" mass="34557" checksum="2B50CD68B6AB7279" modified="2009-02-10" version="1">MGKSLKLVLTLIFICLIIGMFFFLKGVFSGEIILKRYIFKFGFFELRWYSFLIVTGIIISYYLVRRRLKYFDINPDDLDEAVFWGILSSIVGARTYYVIFMWDEFYSKYPSEIIKIWHGGLAIHGAVIGAIVSTFLYTRLKKNCSFTFLQGTDLLTSVLPLGQAIGRWGNFFNYEAYGGPTNLPWKMFVPPENRMVGYENYSYFHPTFLYESIFDFLIFLFLYKYDLKWKKKNGETTALYFILYSANRFWIEGLRTDSLYWGKIRVAQLVSLILIILGIIMFILLRRKTEVKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDU4</accession>
    <name>GLMM_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoglucosamine mutase</fullName>
            <ecNumber evidence="1">5.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glmM</name>
        <name type="ordered locus">THA_1736</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23424"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="5.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Activated by phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.10" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580379.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDU4"/>
    <dbReference type="SMR" id="B7IDU4"/>
    <dbReference type="STRING" id="484019.THA_1736"/>
    <dbReference type="EnsemblBacteria" id="ACJ76171">
        <property type="protein sequence ID" value="ACJ76171"/>
        <property type="gene ID" value="THA_1736"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1736"/>
    <dbReference type="eggNOG" id="COG1109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_7_0_0"/>
    <dbReference type="OMA" id="PHNPEPL"/>
    <dbReference type="OrthoDB" id="1265792at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05802">
        <property type="entry name" value="GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01554_B">
        <property type="entry name" value="GlmM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006352">
        <property type="entry name" value="GlmM_bact"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01455">
        <property type="entry name" value="glmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000201152" description="Phosphoglucosamine mutase">
        <location>
            <begin position="1"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="1">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01554"/>
        </source>
    </evidence>
    <sequence length="430" mass="47844" checksum="F715DE47690A325D" modified="2009-02-10" version="1">MRYFGTDGIRGVVNEFLTPELAFRLGNAVGNMVNGKVFIAKDTRNSGDMLEAALIAGITSAGADVYRCGIMPTPALALITKLEDAAGIMISASHNPPEYNGLKVIMKGYKLPDSLEERIENEMQNVKYNSFEKVGRVIDYRLAEEEYFNYIKELYKNLDLSGIKIVMDVANGATFNLNPKILEYFGAKIEVVNNEPDGFNINKDCGSTHPENIKNYIVNGKIGILHDGDGDRCIFLDENGQEFHGDKIIGLTALQLKKEGRLKNDKVVVTILSNMGLERFLNENSIDVVRTKVGDRYVLEEMLKENITLGGERSGHIIYLDRSTTGDGLITALETLSAMVNSGKRLADLSNLIPDYPQVMLNVKVSDKEVYKSKEVFEKLKSIKDYRVIVRPSGTEPVVRVLVEGPDMDESTIIANDIADLIKKFDNKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDV5</accession>
    <name>B7IDV5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA polymerase sigma factor for flagellar operon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1748</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580385.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1748"/>
    <dbReference type="EnsemblBacteria" id="ACJ76182">
        <property type="protein sequence ID" value="ACJ76182"/>
        <property type="gene ID" value="THA_1748"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1748"/>
    <dbReference type="eggNOG" id="COG1191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_8_1_0"/>
    <dbReference type="OMA" id="IKFETYA"/>
    <dbReference type="OrthoDB" id="1857862at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012845">
        <property type="entry name" value="RNA_pol_sigma_FliA_WhiG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000770">
        <property type="entry name" value="RNA_pol_sigma-SigE/K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02479">
        <property type="entry name" value="FliA_WhiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4" evidence="6">
        <location>
            <begin position="172"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="226" mass="26215" checksum="C74462E7BA396873" modified="2009-02-10" version="1">MINKEALVSEYLPYIKKIAYDLKRNLPHNVEVDDLIQEGLVAFIQAIEKFDPKKGAKLRSYLLTRVKGAMYDYLRKIDWMPKNLRHDIKLVEDAIVNMESDTNDIDFEKIAKITNLPIENVKRAYNELTRKQLLMLDSYISDDSELIDKIASDENPYSYAINELLKEQLIEAIKKLDDKEQLLLSLRFEHELSLKEIGKVLNVTESRVSQLLAKTLAKLKKYLGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE10</accession>
    <name>B7IE10_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thiamine biosynthesis protein ThiH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1806</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580430.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1806"/>
    <dbReference type="EnsemblBacteria" id="ACJ76237">
        <property type="protein sequence ID" value="ACJ76237"/>
        <property type="gene ID" value="THA_1806"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1806"/>
    <dbReference type="eggNOG" id="COG0502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046249_0_0_0"/>
    <dbReference type="OMA" id="LICAYRL"/>
    <dbReference type="OrthoDB" id="419725at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044271">
        <property type="term" value="P:cellular nitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018130">
        <property type="term" value="P:heterocycle biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901362">
        <property type="term" value="P:organic cyclic compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901566">
        <property type="term" value="P:organonitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042364">
        <property type="term" value="P:water-soluble vitamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024007">
        <property type="entry name" value="FeFe-hyd_mat_HydG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034428">
        <property type="entry name" value="ThiH/NoCL/HydG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43583">
        <property type="entry name" value="PTHR43583"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43583:SF2">
        <property type="entry name" value="PTHR43583:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00319">
        <property type="entry name" value="Fe_hydrogenase_maturase_(HydG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03955">
        <property type="entry name" value="rSAM_HydG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="80"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="471" mass="54060" checksum="D646CAA968585095" modified="2009-02-10" version="1">MYVFIKEKDTQKTFIDEKKIKENLSSNLNPDSEKIRNILQKSLDKQRLDPDEVATLLNAKDEKQWEEIFEAARTLKEKVYGNRIVLFAPLYIGNDCINDCEYCGFRISNKEVVRKTLSFEKLKEEVKALVSKGHKRLIVVYGEHPKYSPEFIAKTIDIIYNTKHGNGEIRRVNVNAAPQTVEGYKIIKEVGIGTYQIFQETYHYPTYKKLHPRGPKSNFAWRLYGLDRAMLAGIDDVGIGALFGLYDWKFEVMGLIYHTLHLEERFGVGPHTISFPRIEPAVGTPIAERPPYQVSDEDFKKLVAVLRLAVPYTGLILTAREPADLRREVLKLGVSQIDAGSSIGVGSYSETDPEVIRKSQFILGDTRTLDEVIYELLKEEYIPSFCTACYRAGRTGEHFMEFAIPGFVKRFCTPNALFTLNEYLNDYASEKTYKEGKKVLEKEIKKIDEKQKKIVLDGLERINKGDRDVRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE21</accession>
    <name>PANC_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pantothenate synthetase</fullName>
            <shortName evidence="1">PS</shortName>
            <ecNumber evidence="1">6.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pantoate--beta-alanine ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pantoate-activating enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">panC</name>
        <name type="ordered locus">THA_1817</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantoate + ATP + beta-alanine = (R)-pantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The reaction proceeds by a bi uni uni bi ping pong mechanism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pantothenate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580440.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IE21"/>
    <dbReference type="SMR" id="B7IE21"/>
    <dbReference type="STRING" id="484019.THA_1817"/>
    <dbReference type="EnsemblBacteria" id="ACJ76248">
        <property type="protein sequence ID" value="ACJ76248"/>
        <property type="gene ID" value="THA_1817"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1817"/>
    <dbReference type="eggNOG" id="COG0414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047148_0_0_0"/>
    <dbReference type="OMA" id="CNHKLEP"/>
    <dbReference type="OrthoDB" id="1661843at2"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00005"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004592">
        <property type="term" value="F:pantoate-beta-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00560">
        <property type="entry name" value="PanC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00158">
        <property type="entry name" value="PanC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003721">
        <property type="entry name" value="Pantoate_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042176">
        <property type="entry name" value="Pantoate_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21299:SF1">
        <property type="entry name" value="PTHR21299:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02569">
        <property type="entry name" value="Pantoate_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00018">
        <property type="entry name" value="panC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000118157" description="Pantothenate synthetase">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="30"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>(R)-pantoate</name>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>beta-alanine</name>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="147"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>(R)-pantoate</name>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="184"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00158"/>
        </source>
    </evidence>
    <sequence length="278" mass="32189" checksum="FC012F6191E5DF40" modified="2009-02-10" version="1">MKVIEKIEEMKKISREILESKKAIGFVPTMGFLHEGHLSLVKAAKSENDITVVSIFVNPTQFGPNEDYNNYPRDLERDLSMLKDMEVDYVFVPSVEEMYPDSFSTYVEEIKLSRFLCGASRPGHFRGVCTVVTKLFNIVKPTRAYFGQKDAQQFRVLRRMVRDLNMDVELVEMPIVREPDGLALSSRNTYLNDEERKEAVRLYKSLLKAKELIESGEKDVEIIKNEMKKILTHPLLRIDYIEIVDEENLEPVEKIDRRVIIAIAVFVGRARLIDNMII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE59</accession>
    <name>B7IE59_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exopolyphosphatase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1860</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102845.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1860"/>
    <dbReference type="EnsemblBacteria" id="ACJ76286">
        <property type="protein sequence ID" value="ACJ76286"/>
        <property type="gene ID" value="THA_1860"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1860"/>
    <dbReference type="eggNOG" id="COG0618">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039720_0_0_0"/>
    <dbReference type="OMA" id="YTGIMTD"/>
    <dbReference type="OrthoDB" id="1040743at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="1">
        <location>
            <begin position="18"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="203"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="328" mass="36661" checksum="0D083F7C2D9C4F18" modified="2009-02-10" version="1">MVANNFLNIVANLKQANKVLVLGHIMPDGDDISSVLSLTLGLKKLGKEVIPAIDWKIPSYLGEIDEVLLIKSFEEIDIKNFAPDIIAIVDASSPDRVGRFSQYLNDYNVLVIDHHATNTLFGNINWVDPTFGATAQMILRINRELEVEYDERLALINLLGIETDTGFFRYSNADERVFKDAAALISLGAKPDVIARMVLENKRLEQFKLLSRMIEKLKIESDGLLAYSYLSKEDYEELNCTDEDSGGFVSEIRSIKGVEVAIFFYEYTEGEIHISFRSKEWFDVSKVALLIGGGGHPRAAGASVKGKIEDIVDEVVKLTISEIKNQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEC0</accession>
    <name>B7IEC0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Diguanylate cyclase with PAS/PAC sensor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1923"/>
    <dbReference type="EnsemblBacteria" id="ACJ76347">
        <property type="protein sequence ID" value="ACJ76347"/>
        <property type="gene ID" value="THA_1923"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1923"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2977777_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="58" mass="7043" checksum="5D742F5313173448" modified="2009-02-10" version="1">MSEKLSEIFKLSFQRISDNIFRYGGEEFVIYYLSQNKENFFEILENLRKKVKKNTNTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEE9</accession>
    <name>B7IEE9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endoribonuclease Cas2</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cas2</name>
        <name evidence="10" type="ordered locus">THA_1954</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas1 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580502.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1954"/>
    <dbReference type="EnsemblBacteria" id="ACJ76376">
        <property type="protein sequence ID" value="ACJ76376"/>
        <property type="gene ID" value="THA_1954"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1954"/>
    <dbReference type="eggNOG" id="COG1343">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161124_0_1_0"/>
    <dbReference type="OMA" id="KKYFTWV"/>
    <dbReference type="OrthoDB" id="1951170at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09725">
        <property type="entry name" value="Cas2_I_II_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405">
        <property type="entry name" value="PTHR34405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ76376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="86" mass="10283" checksum="9F63514283B7796D" modified="2009-02-10" version="1">MFVILYYDVNVKRVSKMLKVCRKYLTWVQNSVFEGEISYSNLESLKSEIKKIVKDEDSVVIYVFNQMLYSKREVLGKDKKEETKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEF5</accession>
    <name>B7IEF5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580508.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1960"/>
    <dbReference type="EnsemblBacteria" id="ACJ76382">
        <property type="protein sequence ID" value="ACJ76382"/>
        <property type="gene ID" value="THA_1960"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1960"/>
    <dbReference type="HOGENOM" id="CLU_483059_0_0_0"/>
    <dbReference type="OMA" id="ISLWAND"/>
    <dbReference type="OrthoDB" id="1691660at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="564" mass="67331" checksum="2C0F7AF9A8E0585F" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEG6</accession>
    <name>B7IEG6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-oxoacyl-[acyl-carrier protein] reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1972</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1972"/>
    <dbReference type="EnsemblBacteria" id="ACJ76393">
        <property type="protein sequence ID" value="ACJ76393"/>
        <property type="gene ID" value="THA_1972"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1972"/>
    <dbReference type="eggNOG" id="COG0300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1502785_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="179" mass="20402" checksum="539E96AE46F5D3D2" modified="2009-02-10" version="1">MTFNPHLINCFSPVEINLKSVKRKIESVNKNIRVETLSFDLSNDMEDLIKKLDNYPIDHLINNAGFGWYGEFVNGNKEIYENMISVNIKALTILSYHFSKKFIEKGKGGIINVGSVAGFFPIPHFAVYGATKAYVYSLSYALWAELKKHNVHVMCLAPGKTKTRFFERANMQNSDKTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEI5</accession>
    <name>B7IEI5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1991</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580532.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76412">
        <property type="protein sequence ID" value="ACJ76412"/>
        <property type="gene ID" value="THA_1991"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1991"/>
    <dbReference type="eggNOG" id="ENOG50332JU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132130_0_0_0"/>
    <dbReference type="OMA" id="YWDEDEL"/>
    <dbReference type="OrthoDB" id="1543779at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="119" mass="14452" checksum="4AE443AD2C6F135A" modified="2009-02-10" version="1">MPSQLYISKEKLKNVMRLIKYKGLNNLEPIPIKKINNIIFYTDGHTRAFALWKLGFKKIKVEWETEELDWELYLICIDWCKNKNISNISHLKIISHSQYKKWWIKKCEDMHKLLYPSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEL2</accession>
    <name>B7IEL2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UAA transporter family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_18</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103109.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_18"/>
    <dbReference type="EnsemblBacteria" id="ACJ74526">
        <property type="protein sequence ID" value="ACJ74526"/>
        <property type="gene ID" value="THA_18"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_18"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_969537_0_0_0"/>
    <dbReference type="OMA" id="MVMMAIE"/>
    <dbReference type="OrthoDB" id="1690449at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="3"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="147"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="287" mass="32347" checksum="36D3733ECE3286EF" modified="2009-02-10" version="1">MHYITAIMSAFSSSVTSIFGKLSLNVGASALQILFLRFFFSLIISGLFFVLLKKKINIKYLLFFGIFGIVNYGIAAYLFFYGLRFLNPAYATVLFFSNPIFVLIFQKLIYKGNFSVFNLFAVVLSFTGIILSNLAEKSIGGSENLVFGSLLVILAAIFNALFITLVGDKIKKLQLSPFESSFYTFLGVTILYFIISIANKELYTIKPDYYIYGLLLAVFSTLIPLTLNYFSLRKINSTTLSLIMPLEIVFASILSFIFFDEMFNILKVLGFSFVVVAPILERVKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEL4</accession>
    <name>B7IEL4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_20</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579297.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_20"/>
    <dbReference type="EnsemblBacteria" id="ACJ74528">
        <property type="protein sequence ID" value="ACJ74528"/>
        <property type="gene ID" value="THA_20"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_20"/>
    <dbReference type="eggNOG" id="COG0730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_2_3_0"/>
    <dbReference type="OMA" id="KELKGHW"/>
    <dbReference type="OrthoDB" id="1445898at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="245" mass="26842" checksum="5C7942423A624769" modified="2009-02-10" version="1">MAILLYLFLFFAGISAGFINVLAGGGSMLTLPVLTLLGLDISVANGTNRVGILLQNIIATKNFKKHRLFTLKEGFSIALPATIGAIFGTFTVLNLNKELLEKIIGTIFLFMSFFILYKPKLWEEGKKVKRNYLITFIVFFAIGFYGGFIQAGVGFFLILGLVLVEGNNIIKTNALKVFVVLNYTVFSFLIFLLNGKVDILKGLVLAAGTMIGANLGTKTALKTGAKFVRIFVFLMIVISAIKYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEN0</accession>
    <name>B7IEN0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 6" type="primary">gcvH</name>
        <name evidence="6" type="ordered locus">THA_36</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="3">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579310.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_36"/>
    <dbReference type="EnsemblBacteria" id="ACJ74544">
        <property type="protein sequence ID" value="ACJ74544"/>
        <property type="gene ID" value="THA_36"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_36"/>
    <dbReference type="eggNOG" id="COG0509">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097408_2_2_0"/>
    <dbReference type="OMA" id="YRDSHEW"/>
    <dbReference type="OrthoDB" id="1773485at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="PTHR11715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="14"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="3 4">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617453-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="122" mass="13628" checksum="7B023B264AC382D3" modified="2009-02-10" version="1">MKKFTKTHEWVDENGYVGITSHAQEELGDIVYVDLPEVGKEVKKGDVLLSIESVKAASDVYAPISGKIVEVNNELDARPELVNEDAEGEGWLVKIEISNPSELDELMTEEEYKEFLNSEGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEP0</accession>
    <name>B7IEP0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acriflavine resistance protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_46</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579314.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_46"/>
    <dbReference type="EnsemblBacteria" id="ACJ74554">
        <property type="protein sequence ID" value="ACJ74554"/>
        <property type="gene ID" value="THA_46"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_46"/>
    <dbReference type="HOGENOM" id="CLU_1460642_0_0_0"/>
    <dbReference type="OMA" id="ANIIRNH"/>
    <dbReference type="OrthoDB" id="2000355at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="185" mass="22265" checksum="A924F4D643D611A0" modified="2009-02-10" version="1">MENLNLYKDKEKRISLKRFLIYLIISFLPFFSLYYFSQFFLFTNIANIIRNHPLVFNEISFNELTEIPDIVDLKISEYDSKINSLINKIRFLKTSLNKSFFSKYFLQVLVNELSNINEYFFIKNIEFDGNSFLITFYEYSEKPVVPETIKEKLSKYYKNVELKVTDIKNVYENFKMYELQLGGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IET1</accession>
    <name>B7IET1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase, gnat family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_87</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103443.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_87"/>
    <dbReference type="EnsemblBacteria" id="ACJ74595">
        <property type="protein sequence ID" value="ACJ74595"/>
        <property type="gene ID" value="THA_87"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_87"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_19_1_0"/>
    <dbReference type="OMA" id="FGMGVKD"/>
    <dbReference type="OrthoDB" id="1809950at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="172" mass="20312" checksum="75830506DF92B759" modified="2009-02-10" version="1">MFNTELIIEEISKSDIFNLYEYRKNILSETPFLVTTLDDLMDFNSFKNYIHFYIENDLRNIFVAKKDNTIIGEITILIHDKKRMRHVAEFGISVLKEYRGKGIGRKLIQTAEKWAFDKNVKRIQIEVMENNNVALNLYLSLGYKIEGRKKMAVRFGDSFVDLIIMAKLRDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IET9</accession>
    <name>B7IET9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Probable diacylglycerol kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">THA_95</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103474.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_95"/>
    <dbReference type="EnsemblBacteria" id="ACJ74603">
        <property type="protein sequence ID" value="ACJ74603"/>
        <property type="gene ID" value="THA_95"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_95"/>
    <dbReference type="eggNOG" id="COG0818">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112343_2_1_0"/>
    <dbReference type="OMA" id="SFRYAAQ"/>
    <dbReference type="OrthoDB" id="2028997at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14265">
        <property type="entry name" value="UDPK_IM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000829">
        <property type="entry name" value="DAGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036945">
        <property type="entry name" value="DAGK_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033717">
        <property type="entry name" value="UDPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34299">
        <property type="entry name" value="PTHR34299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01219">
        <property type="entry name" value="DAGK_prokar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ74603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="159" mass="18336" checksum="6A36866CC6917D29" modified="2009-02-10" version="1">MKEKLNGRLASNNLKESFEHAIEGIIYALSSERNLRIHFFVGFVILFITLFLPIDKNNYIWIFFAVFFVIISELINTLIEKILDLFVPEYHPIVKVIKDISSGVVLWAALFSVVVGIAIIGNYLFNWNVLIAKVVGFVFVTAFPLIYLILGVRKWKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEU2</accession>
    <name>B7IEU2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Deoxycytidylate deaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_98</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103479.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_98"/>
    <dbReference type="EnsemblBacteria" id="ACJ74606">
        <property type="protein sequence ID" value="ACJ74606"/>
        <property type="gene ID" value="THA_98"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_98"/>
    <dbReference type="eggNOG" id="COG2131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047993_2_3_0"/>
    <dbReference type="OMA" id="TKWYVLH"/>
    <dbReference type="OrthoDB" id="1544522at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004132">
        <property type="term" value="F:dCMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008251">
        <property type="term" value="F:tRNA-specific adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01286">
        <property type="entry name" value="deoxycytidylate_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016473">
        <property type="entry name" value="dCMP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015517">
        <property type="entry name" value="dCMP_deaminase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035105">
        <property type="entry name" value="Deoxycytidylate_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028883">
        <property type="entry name" value="tRNA_aden_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086">
        <property type="entry name" value="PTHR11086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14437">
        <property type="entry name" value="MafB19-deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006019">
        <property type="entry name" value="dCMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="7">
        <location>
            <begin position="21"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR006019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="170" mass="18957" checksum="DC7A6E38D756CF62" modified="2009-02-10" version="1">MNLDSYLKNLKITISMDKRESWDSYFLKIAEIIATRSTCFHRKVGAVIVKDKRILATGYNQPPSGFPHCDDIGCIRDDLNIKSGENQEVCYALHAEQNALMQAAKFGISTDGATIYVTHKPCSVCARLIINAGIKKVVYLNDYPDSLTDFLFKTSKISVEKHGGVIHEKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEV2</accession>
    <name>METK_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-adenosylmethionine synthase</fullName>
            <shortName evidence="1">AdoMet synthase</shortName>
            <ecNumber evidence="1">2.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">MAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Methionine adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metK</name>
        <name type="ordered locus">THA_108</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579352.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IEV2"/>
    <dbReference type="SMR" id="B7IEV2"/>
    <dbReference type="STRING" id="484019.THA_108"/>
    <dbReference type="EnsemblBacteria" id="ACJ74616">
        <property type="protein sequence ID" value="ACJ74616"/>
        <property type="gene ID" value="THA_108"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_108"/>
    <dbReference type="eggNOG" id="COG0192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041802_1_1_0"/>
    <dbReference type="OMA" id="MPYLRPD"/>
    <dbReference type="OrthoDB" id="1024388at2"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00086">
        <property type="entry name" value="S_AdoMet_synth1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="PTHR11964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="SSF55973"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000196734" description="S-adenosylmethionine synthase">
        <location>
            <begin position="1"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Flexible loop" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="175"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="242"/>
            <end position="243"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="257"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00086"/>
        </source>
    </evidence>
    <sequence length="395" mass="43442" checksum="C97CAEF62B079E17" modified="2009-02-10" version="1">MKRLFTSESVTEGHPDKVADQISDAILDAMLEQDPKSRVAVETLVTTGIAIVSGEVTTRAYVDIQDIVRKTILDIGYTRAKYGFDGETCAVLSSIHSQSPDIALGVDKALEAKEGELIAEDELEQVGAGDQGMMFGYATNETKEYMPLPIMLSHKLAMKLSEVRKNGTLPFLRPDGKTQVTIEYDENDKPVRVDTVLISTQHEPDVTISEIKEALIKHVIDPIIPEELRDDKMKILVNPTGRFVLGGPSADTGLTGRKIIVDTYGGAVPHGGGAFSGKDPTKVDRSAHYFARYVAKNVVAAGLADKFMIQVAYAIGKAQPVSVMINTFGTAKTDEDKILKAILEIFDFRPGAIIKKLDLLRPIYKKTAAYGHFGRELEEFTWEKLDMVNELKKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEW2</accession>
    <name>B7IEW2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sensor histidine kinase, PhoR-related</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_118</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579359.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_118"/>
    <dbReference type="EnsemblBacteria" id="ACJ74626">
        <property type="protein sequence ID" value="ACJ74626"/>
        <property type="gene ID" value="THA_118"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_118"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_2_0"/>
    <dbReference type="OMA" id="EPQWSAK"/>
    <dbReference type="OrthoDB" id="1827824at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="109"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="316" mass="36981" checksum="7DC8AA8CC0D8D86E" modified="2009-02-10" version="1">MFNFEIFQNFDEPIIMLDGLKIAKANNKALEYGFKENFEILDVFTSKNIDVIIEYLIERKNFELEEKLYFFEFSDWRYVKIKFFDNLLYLSDLTEYVKIKEAKVNFTTAISHELFNPLSIIKANTIYLKDTLNENNKDILEALEDIEKSSNRMERIIKQLKVLAMLELGMYKPKIDIVDFEKILNEVIDELSQKLKSKNLTIDLKILNKEYKSDGFMLYTIIKNVLSNSIKYSYNNSTIIIEAYKDKIIISDSGIGIKKEDLEKVTQRFYRSKEATKMATGSGLGLSIVKHICNILNYKLIIESNYLIGTKVIIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEW7</accession>
    <name>B7IEW7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphate ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_123</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103529.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_123"/>
    <dbReference type="EnsemblBacteria" id="ACJ74631">
        <property type="protein sequence ID" value="ACJ74631"/>
        <property type="gene ID" value="THA_123"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_123"/>
    <dbReference type="eggNOG" id="COG0573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_1_0_0"/>
    <dbReference type="OMA" id="VIRMSVL"/>
    <dbReference type="OrthoDB" id="1325025at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="61"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="67"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="286" mass="31738" checksum="5C13F720E1688AD4" modified="2009-02-10" version="1">MRTFKYYFQMFLIYFFALLSITALFLLIVFIFKEALPAIVELKLKPLMSVYWYPTYDPPEFGMLALIADTLLVTLVSSIITIPIGYFIAFYLHTYSNGFEKRFIKFIISILSGIPSVIIGMFLVFYISPIFLNFGVWSTENFLLAVIGLSLLSLPYSVSLMTDSLDSVPKELIESSLSLGVSKFITTLKITTLASRSGLINSTILTINRIIGETMVVLLVGGGAAMIPTSFFDPIKPLTAAIASEIGEAGVGSMHYHSLFLAGLILLVISLCLTYLSKLRSWKTYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEZ7</accession>
    <name>B7IEZ7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyridoxal 5'-phosphate synthase subunit PdxS</fullName>
            <shortName evidence="3">PLP synthase subunit PdxS</shortName>
            <ecNumber evidence="3">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Pdx1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pdxS</name>
        <name evidence="6" type="ordered locus">THA_154</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = H(+) + 3 H2O + L-glutamate + phosphate + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">In the presence of PdxT, forms a dodecamer of heterodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103611.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_154"/>
    <dbReference type="EnsemblBacteria" id="ACJ74661">
        <property type="protein sequence ID" value="ACJ74661"/>
        <property type="gene ID" value="THA_154"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_154"/>
    <dbReference type="eggNOG" id="COG0214">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055352_1_0_0"/>
    <dbReference type="OMA" id="RYANRGW"/>
    <dbReference type="OrthoDB" id="784095at2"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PTHR31829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00343">
        <property type="entry name" value="TIGR00343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01235">
        <property type="entry name" value="PDXS_SNZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0704">Schiff base</keyword>
    <feature type="domain" description="SOR_SNZ" evidence="5">
        <location>
            <begin position="7"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribose 5-phosphate binding" evidence="3">
        <location>
            <begin position="235"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="3">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="292" mass="31587" checksum="549CC917D5427C8A" modified="2009-02-10" version="1">MTQKGTWMVKEGFAEMFKGGVIMDVTTAEQAKIAEEAGAVAVMALERVPADIRKAGGVARMASISKIKEIMEAVSIPVMAKVRIGHIAEARILEALGVDFIDESEVLTPADDKYHINKWDFKVPFVCGARNLGEALRRIAEGAAMIRTKGEAGTGNVVEAVKHMRTVMDEIRMVQNMPDEELVTFAKNIGAPVNLVAKVKELGRLPVVNFAAGGVATPADAALMMMLGADGVFVGSGIFKSKDPAKMAKAIVMAVTYWNDPEMLLKISEDIGQPMEGLEIEELDVKLQERGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF10</accession>
    <name>B7IF10_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_169</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAE transporter (TC 2.A.81) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579385.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_169"/>
    <dbReference type="EnsemblBacteria" id="ACJ74674">
        <property type="protein sequence ID" value="ACJ74674"/>
        <property type="gene ID" value="THA_169"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_169"/>
    <dbReference type="eggNOG" id="COG2985">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035023_4_0_0"/>
    <dbReference type="OMA" id="AMWLIRL"/>
    <dbReference type="OrthoDB" id="1458740at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006512">
        <property type="entry name" value="YidE_YbjL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06826">
        <property type="entry name" value="Asp-Al_Ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01625">
        <property type="entry name" value="YidE_YbjL_dupl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Asp-Al_Ex" evidence="7">
        <location>
            <begin position="8"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Asp-Al_Ex" evidence="7">
        <location>
            <begin position="207"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="380" mass="41704" checksum="00A56A1860CB7ECA" modified="2009-02-10" version="1">MSNPFFLLFISILSGLLLGKIQFKNFKLGASGTLFAGLFFGWLFPLFINSQKELINIQNTLNTFFNFSLILFVSSIGLIASRDIKKILKTHGFKFIILGFLITFSGFISTLLFTYFTNLNNLNLLGVFSGSMTSSPGLASALESIKEGSADIIYGYTVGYIPGVLAVIFSIYFIPIIFKININKEKESYKFESIDNSKDYNFNFISFSIVIILGILIGNLNLNLKFASLKLGNTGGILLSSLFLGSLGKIGNLSFEFNKNILKVFQNLGLVIFLSSIGLRSGYKVISNFNRESLYLMVISFVCAIFSILVGYIIGKYIFKLDWIILAGAITGGMTSTPGLGAALDSTKSELVTAGYGATYPFALLGMVIFNKLFSILTNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFB0</accession>
    <name>B7IFB0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TldD protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_274</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103860.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_274"/>
    <dbReference type="EnsemblBacteria" id="ACJ74774">
        <property type="protein sequence ID" value="ACJ74774"/>
        <property type="gene ID" value="THA_274"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_274"/>
    <dbReference type="eggNOG" id="COG0312">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_1_0_0"/>
    <dbReference type="OMA" id="TDFWGSM"/>
    <dbReference type="OrthoDB" id="806701at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD" evidence="3">
        <location>
            <begin position="22"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_M" evidence="5">
        <location>
            <begin position="113"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_C" evidence="4">
        <location>
            <begin position="228"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="462" mass="50468" checksum="EDB069DADC79A666" modified="2009-02-10" version="1">MLNQSLIKDIIGTVLKYGGDFAEVFVENKYENRIELTDGKIQKANTNNISGVGIRGFLGKKAIYAYTNVFEKENLLSVAKRVGEALSEVKIDDLTLDFDNKKLKNRHVIYLYPRDVEKTEKVKVMKRAYESAKNYSELIKQVQVWFWEYDQEVLIANSEGVWAEDRRVKTRLMINAIAEHNGNMERGFYGPGASMGFELFDRIDVEEAAKRAARIAARMVDAEPAPAGKMPVIISNEFGGVIFHEAVGHALEATSVAKGASVFAGKLGQKVAAECVSAVDDATIPNAWGSANVDDEGTPTRRNLLIDKGVLVGYLIDKLGGRRMGMESTGSARRQDYTFAPTSRMSNTFILPGDYHPEEIIAATEYGLYAKEMGGGSVNPSTGEFNFAVNEAYLIEKGKITKPVKGATLIGKGYEIIQKIDMVGNDVGRGQGVCGSISGAVPADVGQPTIRVSEIIVGGRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFF2</accession>
    <name>B7IFF2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Crispr-associated protein, family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579495.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_316"/>
    <dbReference type="EnsemblBacteria" id="ACJ74816">
        <property type="protein sequence ID" value="ACJ74816"/>
        <property type="gene ID" value="THA_316"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_316"/>
    <dbReference type="eggNOG" id="COG1517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_570994_0_0_0"/>
    <dbReference type="OMA" id="ILIAPWG"/>
    <dbReference type="OrthoDB" id="2139533at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010171">
        <property type="entry name" value="CRISPR_Csx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019016">
        <property type="entry name" value="Csx1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09455">
        <property type="entry name" value="Cas_DxTHG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01897">
        <property type="entry name" value="cas_MJ1666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="478" mass="56552" checksum="F47911CF48C4E07C" modified="2009-02-10" version="1">MKLFFSTFGKISNYKEVEYSIDYNENTFESPFLPDLMCKAFGVDTTELFLLDTLVEKNDLLKEDFKYEDIITSLENKYFNYFKSKDYKGNLNLHIIPGVGSYKEFRFVGNMNDTYYMTLIQLLNIFSRPEVINSEEIEVILDISFGINYQSNVLYRTLMDILPVLAYFKKVRFKLTNAEPVINTSAEGKNKINVYVVEEKVFEQSISYKKDFAKKTISVFRGLSINERKEVGKNYIREFEKRLRNEKNMGLNNVFLNLELYLKSIYYGLPLLYLYAMNGADFKYVVDKSLEMYNSAIEIRDSNENLKMVLRKLEFSEGIKYLVLGSIFKIYFENIYGTKYCTEIPYDELKKLSNFIWKIDEFKNQISLVLEKEIKEIESLFDYIEEGEEILFDLEMLKSRKNESIECEEKVDENTKKELEHENDELKKQNSIENFSRKERNFIAHGSLSKNTFYLKKYNSMVCIRLNEEEICKLGKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFG2</accession>
    <name>B7IFG2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transporter, major facilitator family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_326</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579501.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_326"/>
    <dbReference type="EnsemblBacteria" id="ACJ74826">
        <property type="protein sequence ID" value="ACJ74826"/>
        <property type="gene ID" value="THA_326"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_326"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035564_0_0_0"/>
    <dbReference type="OMA" id="WQAYFLK"/>
    <dbReference type="OrthoDB" id="1345431at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="1"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="400" mass="45232" checksum="71DBA987468CCF0F" modified="2009-02-10" version="1">MIKKDKQFRKFQMYGFLKNLRFFEPFLILFFLSSGLSYLQIGILYSIKSIATNILEIPTGVVADLYGRKKSMLFSMISYIVSFLIFYFSRSFYLYILAMIFFSFGEAFRTGTHKAMIFQYLKINGMENLKVQYYGATRAASQLGSALNSLIAASIVFVSGNYRTVFFIAILPYILNLINLATYPNELDKAEVKKTKKQTLKDFIDAVASWRVLKILLNTSSYSAIFKSLKDYLQPILESFALSIPIFVWLDDKKRSSIVIGIVYFFLYLLTSYASKNSWKIGKKSSSFALNLTLFVGALSVIFAGVSYIFNFYIVSILIFIVLYILENLRRPIAVSYVGDNVESDALASVLSVESQATTLLTAVFSIVLGFFADKLGVGQAVSILGIILLLMSLFFKIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFH2</accession>
    <name>B7IFH2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-oxoglutarate oxidoreductase, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">vorB</name>
        <name evidence="3" type="ordered locus">THA_336</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579508.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_336"/>
    <dbReference type="EnsemblBacteria" id="ACJ74836">
        <property type="protein sequence ID" value="ACJ74836"/>
        <property type="gene ID" value="THA_336"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_336"/>
    <dbReference type="eggNOG" id="COG0674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_0_0_0"/>
    <dbReference type="OMA" id="DFIQGDE"/>
    <dbReference type="OrthoDB" id="360732at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR_N" evidence="1">
        <location>
            <begin position="14"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="PFOR_II" evidence="2">
        <location>
            <begin position="248"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="352" mass="39032" checksum="9DBA83842C012AAC" modified="2009-02-10" version="1">MAKVMVKGTEAIGEAAIRAGCRHFFGYPITPQSELPEYMSKRLPEVGGVFLQTESEVATVNMLYGAACTGKRVMTSTSSPGFSLMMEGVSYMACAKLPAVFVNVVRGGPGLGDIQPAQGDYWQATKGGGHGDYRLIVLAPSTVQEAVDLTVLAFDLSDKYRTPALILADGMLGQMMEPVEFPEFRDLSTLPDHSDWALTGAKGREPHIVTSFDIDPYNLEKMNLELVEIYKEIEKNEVRWEEYRTEDADIVITAFGTVARIAKSVVDMAREDGIKVGLFRPITVWPYPYEPLEKLADKVEMFFDVEMNMGQMLEDVKLAVKGKKPIKFYSRMGGVVPTPNEIYDALKKEIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFI5</accession>
    <name>B7IFI5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein SsnA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104030.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_350"/>
    <dbReference type="EnsemblBacteria" id="ACJ74849">
        <property type="protein sequence ID" value="ACJ74849"/>
        <property type="gene ID" value="THA_350"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_350"/>
    <dbReference type="eggNOG" id="COG0402">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_2_6_0"/>
    <dbReference type="OMA" id="CYEVSDR"/>
    <dbReference type="OrthoDB" id="1592010at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="1">
        <location>
            <begin position="49"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="427" mass="48323" checksum="807B555925DF6C4B" modified="2009-02-10" version="1">MKAIINAKIFDYENFHENYYIIFDKKIIDIGSMENFKGAPQTIDAKGNFVLPGFVIGHTHIYSTFARGLNLPFNPLNFKDILTQLWWKLDSQLAKEENYYSALVAGIEFIKNGVTTVIDHHASGLQIKGSLNTLKKALCDEVGLRGIFCFETSDRFNVDECIEENLEFLKTRSEMHVGLFGLHASLSLSDNTLEKVSKLYKGPIHIHTAESIDDVEDSLSKYGLRVIPRLEKFGLLRENSILAHCVHTSEEELELISKNNCFVALNVTSNMNNAVGLPNYKLMKKHNVKVIAGNDGLGFNFARELLNLYFSMKLKGNSPLSFSFDDLKSIISNTYEIASKHLNVKLGKIKPGYAADLIIVPYTPPTPLNSENAFGHVIYGLFDNFRPSHVIVNGKILMQNYETNLQVNEIYKQARKVAEYLWKKLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFJ5</accession>
    <name>B7IFJ5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable tRNA sulfurtransferase</fullName>
            <ecNumber evidence="6">2.8.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Sulfur carrier protein ThiS sulfurtransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Thiamine biosynthesis protein ThiI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA 4-thiouridine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">thiI</name>
        <name evidence="9" type="ordered locus">THA_365</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + ATP + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:13337"/>
            <dbReference type="Rhea" id="RHEA-COMP:13338"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136798"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase]</text>
            <dbReference type="Rhea" id="RHEA:43340"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12908"/>
            <dbReference type="Rhea" id="RHEA-COMP:12910"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:90619"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ThiI family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.4" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579525.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_365"/>
    <dbReference type="EnsemblBacteria" id="ACJ74859">
        <property type="protein sequence ID" value="ACJ74859"/>
        <property type="gene ID" value="THA_365"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_365"/>
    <dbReference type="eggNOG" id="COG0301">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037952_4_0_0"/>
    <dbReference type="OMA" id="SMPEFCG"/>
    <dbReference type="OrthoDB" id="773697at2"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140741">
        <property type="term" value="F:tRNA U4 sulfurtransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01712">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00021">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003720">
        <property type="entry name" value="tRNA_STrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00342">
        <property type="entry name" value="TIGR00342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="8">
        <location>
            <begin position="56"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="179"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="204"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="389" mass="44242" checksum="3B235F72EC17495E" modified="2009-02-10" version="1">MEPVVVVRYSEIGLKGKNRGYFEKKLIDNIRKIARPPEINKRYGRIIIRLKDMDFDEIKERLKYVFGIQNFSFAYAVSHDLDKIKEVVLKLLKLKLKNEKTFKVQTKRSYKQFPMNSQELSAHIGAFVLENFKELSVDVHNPDIIVGIEVKEKEVFVFVGKEQMYGGFPVGSSGKGILLLSGGIDSPVAGWYMMKRGILIETLSFLSPPFTSEKSKNKILELSNILSKYVPDTLKTWIVPFTPVQQYIKTNAPDKYSLIIQRRSMMRIANKLAKKIKAKAIITGENVGQVASQTLENLHTIESASNLPVLRPLIGFEKIDIVEKAKEIGSYEISIQPYLDSCVVFAPKNPATKSSIKEIEEIESKLTELSNLEEKAFENIEKHVIGRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFL1</accession>
    <name>B7IFL1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579537.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_382"/>
    <dbReference type="EnsemblBacteria" id="ACJ74875">
        <property type="protein sequence ID" value="ACJ74875"/>
        <property type="gene ID" value="THA_382"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_382"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077889_0_0_0"/>
    <dbReference type="OMA" id="QFTYKPT"/>
    <dbReference type="OrthoDB" id="1160025at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029059">
        <property type="entry name" value="AB_hydrolase_5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12695">
        <property type="entry name" value="Abhydrolase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_5" evidence="1">
        <location>
            <begin position="63"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="240" mass="26994" checksum="8D308F5815CADBEA" modified="2009-02-10" version="1">MKKALLISTSIILAFIFIFLIWSINPLKPLSEVENYLKSSEKVEIEYIGKDIFFKPKTNSNIGIIFYPGGHVDYRAYAPLGFYLAKNNINFVLLKMPLNLAFFRTNGAKSIIDKYGNIKWYVAGHSLGGIAAIEFAKKNNVEGVILLASYPAKDISNLELKILAITASNDGLVTQEKFKSKIKLFPKNTVFYEIDGGNHSQFGFYPLQKGDNEATIDRYKQLSTIVNKILEFINENSSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFL2</accession>
    <name>B7IFL2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">K+ channel, beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_383</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104097.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_383"/>
    <dbReference type="EnsemblBacteria" id="ACJ74876">
        <property type="protein sequence ID" value="ACJ74876"/>
        <property type="gene ID" value="THA_383"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_383"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_0"/>
    <dbReference type="OMA" id="PPYSLFW"/>
    <dbReference type="OrthoDB" id="1351747at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="PTHR43150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01577">
        <property type="entry name" value="KCNABCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0407">Ion channel</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="2">
        <location>
            <begin position="15"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="320" mass="36487" checksum="E8259BC521BA0BEC" modified="2009-02-10" version="1">MNYRKVGKWGLKISELSLGSWLTFGNQLDIAGAKEIVRESFKNGINFFDTAEAYANGMAESMLGEVLKEFRRSDIVVSTKIFWGGNGPNDKGLSRKHLLEGTWASLKRLQLDYVDIVYCHRPDPETPIEETVLAMDYIVRNGLALYWGTSEWSAEQLEAAHKACKELNCIPPIVEQPQYNMLVRKRVEEEYKPIYEKYGMGLTIWSPLASGILTGKYNNGIPENSRLARFPHLRKHLEENGLLGEKVFKKLQKLQKIADELDAKMSQLAIAWCLLNDNVSSVILGVSSKEQLHENLKAIEIKEKITEDLEKQIREILEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFQ6</accession>
    <name>B7IFQ6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA mismatch repair protein MutS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_427</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579572.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_427"/>
    <dbReference type="EnsemblBacteria" id="ACJ74920">
        <property type="protein sequence ID" value="ACJ74920"/>
        <property type="gene ID" value="THA_427"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_427"/>
    <dbReference type="eggNOG" id="COG0249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041770_0_0_0"/>
    <dbReference type="OMA" id="PHFYIYD"/>
    <dbReference type="OrthoDB" id="1419510at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="PTHR11361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="SSF48334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_MISMATCH_REPAIR_2" evidence="4">
        <location>
            <begin position="253"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="135"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="453" mass="52822" checksum="D27986F6598ADD84" modified="2009-02-10" version="1">MNTGFEYIRKQLDFSSPLGKKAFENIKIINSKEEIEKNLQAIEVFNNIEEKNIEKIKQILSHIQDIEETIKKLNFECTLDEIELFQIKVFSIFSQDLLKLLNTLEIYNIFNLENIEEVIEILDPRGERLPTFYIYEEYDKNLKEIRKIKESENDESKLLEILSREKEIEREICEKLSKKLHKFSNKLLKNFRIISFLDLSISKYELSKKLNLKMPQINSSFEIIYEKLFNPEVENLLNERGKKFQKIDITLNPGTQIITGANMGGKTVLLRTIALSQMMFQMGFYVPAEFSKLPIFDEIFFVSGDFQNIKLGLSSFAAEMEIINNIYNEIKSGKKILILLDEPARTTNPTEGSAIVKTISNLFNRGNVICVISTHFDNVIDKEMRHLRVKGLKDVEKIDPSKDIQDYFDYSVEEVQLYNPPKEALKILKILKIDNDIVSKVEEVLTDEKQIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFR0</accession>
    <name>B7IFR0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Macrolide export ATP-binding/permease protein MacB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_431</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579576.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_431"/>
    <dbReference type="EnsemblBacteria" id="ACJ74924">
        <property type="protein sequence ID" value="ACJ74924"/>
        <property type="gene ID" value="THA_431"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_431"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_0"/>
    <dbReference type="OMA" id="GIMNITF"/>
    <dbReference type="OrthoDB" id="768211at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="20"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="299"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="393" mass="43640" checksum="27D0A8241BF91C41" modified="2009-02-10" version="1">MIYEMFKEAIRSLMHNKMRSFLSMLGIVIGVMAVIIVLSLGNGATYSVKSEIESIGSNVFFVMAKRTKYGKLTLDDIEEMKRQAMYLTDITPSFSSNGNFKYAENTISTQFYATNPDFINIFGLKVESGRLINELDFEGGLKVAVIGSYVAEELFGEEDPLGKEIKLYINNRFLPLKVVGVLEETGSKLFLNVDNVVFIPFTTVEQRLLKLDTVNQFFAKAKNSEVVEQAKDELEIFLFSRLKDEDSYKILSQDEILDTINQVTGMLNLVLGAIAGISLLVGGIGIMNIMLVSVTERTREIGIKKAIGAKNSNIMLQFLIESSILTVSAGIIGVIMGTVFSNFIGKFINVTPHFRVDQIILSFVISGLIGLFFGIYPAIKASKLNPVDALRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFY2</accession>
    <name>B7IFY2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_507</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100674.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_507"/>
    <dbReference type="EnsemblBacteria" id="ACJ74996">
        <property type="protein sequence ID" value="ACJ74996"/>
        <property type="gene ID" value="THA_507"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_507"/>
    <dbReference type="eggNOG" id="COG0592">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_753866_0_0_0"/>
    <dbReference type="OMA" id="NCAKVEI"/>
    <dbReference type="OrthoDB" id="655860at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="343" mass="39896" checksum="817D00222834ACEE" modified="2009-02-10" version="1">MRVFLSVEELKRVLKLIDKTVGTVEKTNRIVGFKYENGFLNLYGSDGCLILKAKVSEMNPTTLSFSVPLDILKFFVFELSGNISIYSDGNYVVLKAKDENLKLKTERFSFKNFEERYEELINVPINTFVNDLDFVSSHLEEGSYVDFYFGNKVRMVAENGSIINYVVRDKKSKEYSWRIPYYSARHLIKALSEIKSGELIVGEGLNKLVLSSDYLFNVCGEELDHLDLVPLENEIEKSEEFLRVSLKSIRRFLRRAMISGRYSRLKIFGNEKGLYFYAFSENMEYRGHIDISSKYIFQTTTNAYFLRSALNRIGSETLLFFKGEKYLIISTMQKRRFILLPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFY5</accession>
    <name>B7IFY5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dihydroorotate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pyrD</name>
        <name evidence="8" type="ordered locus">THA_510</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579624.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_510"/>
    <dbReference type="EnsemblBacteria" id="ACJ74999">
        <property type="protein sequence ID" value="ACJ74999"/>
        <property type="gene ID" value="THA_510"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_510"/>
    <dbReference type="eggNOG" id="COG0167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042042_0_1_0"/>
    <dbReference type="OMA" id="SCPHAKG"/>
    <dbReference type="OrthoDB" id="1109542at2"/>
    <dbReference type="UniPathway" id="UPA00070"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01037">
        <property type="entry name" value="pyrD_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHO_dh" evidence="7">
        <location>
            <begin position="3"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="272" mass="29901" checksum="059A2B7A07012B5F" modified="2009-02-10" version="1">MNLNPPLVIASGPGGSGEYLSLINPKYVGAYTLKTITLNPKSGNKTKRMENSENYIINSIGLENPGIEYVISNIEKFVLKETKTILSLGGDSKDEYIKIAEKIAPYAKKFEAIEFNFSCPNVKKGGLSILSDINEWQEILENVRKILPDSFLIAKLSIEGNFVEVLSKKVKEFGWNGLTLINCVRGLLIKDGKIVMGGLSGPILKPIALRAIYEVRKVLKDIYIIASGGIYTKKDVDDFLLVGANAISIGSALFKDPQVVEELGKYLKGVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG02</accession>
    <name>B7IG02_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FecR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579631.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_528"/>
    <dbReference type="EnsemblBacteria" id="ACJ75016">
        <property type="protein sequence ID" value="ACJ75016"/>
        <property type="gene ID" value="THA_528"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_528"/>
    <dbReference type="eggNOG" id="COG4254">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_382997_0_0_0"/>
    <dbReference type="OMA" id="RITYPIM"/>
    <dbReference type="OrthoDB" id="102343at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FecR" evidence="1">
        <location>
            <begin position="142"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="673" mass="74986" checksum="A7C713B7FEF383E0" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG12</accession>
    <name>B7IG12_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transporter, small conductance mechanosensitive ion channel (MscS) family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_538</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100623.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_538"/>
    <dbReference type="EnsemblBacteria" id="ACJ75026">
        <property type="protein sequence ID" value="ACJ75026"/>
        <property type="gene ID" value="THA_538"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_538"/>
    <dbReference type="eggNOG" id="COG3264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037945_1_0_0"/>
    <dbReference type="OMA" id="GMQDTLT"/>
    <dbReference type="OrthoDB" id="1903165at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="PTHR30221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="SSF82689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="SSF82861"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="275" mass="31097" checksum="7EB0A8CFA8A89B36" modified="2009-02-10" version="1">MKEIFQNFWDEVLLSIISVLVGYVVYKYLTKIIQKSLEAFGKELKAPKTVKFFVGVIISVFVILALLSIWKVNLVPYITGLGISSIIVGLALQEPLTNFVSGILVMSTRKLFEGEVVDINGVTGVVDEIKINHSYIKSFDGKLILIPNKSVWSGIVTKFWPGPVRRVTMDVGVSYNTDLETAFKLLQKAIEEEPLVVKEGVSNFIAFKQFGASSIDFTVYFWVERSTYFDAVNSLAFRIKKIFDENNIEIPFTQIDVHLDYQNLGKVFISEDKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG14</accession>
    <name>B7IG14_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endopeptidase La</fullName>
            <ecNumber evidence="1">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_540</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100612.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_540"/>
    <dbReference type="EnsemblBacteria" id="ACJ75028">
        <property type="protein sequence ID" value="ACJ75028"/>
        <property type="gene ID" value="THA_540"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_540"/>
    <dbReference type="eggNOG" id="COG1067">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014785_0_1_0"/>
    <dbReference type="OMA" id="DWVYVNN"/>
    <dbReference type="OrthoDB" id="128102at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041699">
        <property type="entry name" value="AAA_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13654">
        <property type="entry name" value="AAA_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Lon proteolytic" evidence="3">
        <location>
            <begin position="550"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="124"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="640"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="786" mass="89511" checksum="C9016B568355E038" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG24</accession>
    <name>B7IG24_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_550</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3 7">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100596.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_550"/>
    <dbReference type="EnsemblBacteria" id="ACJ75038">
        <property type="protein sequence ID" value="ACJ75038"/>
        <property type="gene ID" value="THA_550"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_550"/>
    <dbReference type="eggNOG" id="COG0334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_1_2_0"/>
    <dbReference type="OMA" id="PCFAAFP"/>
    <dbReference type="OrthoDB" id="1184827at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004353">
        <property type="term" value="F:glutamate dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELFV_dehydrog" evidence="8">
        <location>
            <begin position="191"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="6">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="427" mass="47026" checksum="61A2468A64C889D2" modified="2009-02-10" version="1">MRLSELGRLEAPGPLYKMAQNQFLRAAKLMDLDPNIGNFLLWPQKSLIVHFPVVMDDGRVEIFEGYRVQHNTARGPAKGGIRYHPETNLDEVSSLAFWMTWKCAVVNLPYGGGKGGVRVDPRKLSEKELEKLSRRFFSEIQMMVGPTKDIPAPDVNTNAKIMAWFMDTYSMNTGNTTLGVVTGKPLDLGGSEGRPEATGRGVSITAAEACKAKGMDISKATVAVQGFGNVGSYAAKILHEEYGAKIVAVSDVSGGLYCEEGFDVNDLIRYRDENGGVIKGYPKGKPISNEELLTLDVDILVPAALENAINGEIAKDVRAKIIVEGANGPTTEEAEKILIEKDVLIVPDILANAGGVTVSYFEWVQDLQSFFWDIDDIRKKLHRIMTKSFSEVYATKEKYNTDMRTAAYIVAISRVAEAVKKRGYFPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG32</accession>
    <name>B7IG32_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100582.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_558"/>
    <dbReference type="EnsemblBacteria" id="ACJ75046">
        <property type="protein sequence ID" value="ACJ75046"/>
        <property type="gene ID" value="THA_558"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_558"/>
    <dbReference type="HOGENOM" id="CLU_2774562_0_0_0"/>
    <dbReference type="OrthoDB" id="2005136at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="69" mass="8323" checksum="76B07BF155101868" modified="2009-02-10" version="1">MRNTPYYWLVLLGEKEYHILPILFVPSQSHVDNILETIYKNKLIDFEVKKVMFSDNFEAVKRVTEKYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG66</accession>
    <name>B7IG66_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579667.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75080">
        <property type="protein sequence ID" value="ACJ75080"/>
        <property type="gene ID" value="THA_593"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_593"/>
    <dbReference type="eggNOG" id="ENOG50331HZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1007630_0_0_0"/>
    <dbReference type="OMA" id="VQFFEME"/>
    <dbReference type="OrthoDB" id="1710746at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025491">
        <property type="entry name" value="DUF4382"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14321">
        <property type="entry name" value="DUF4382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4382" evidence="1">
        <location>
            <begin position="27"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="270" mass="30168" checksum="74FEB593EE26713A" modified="2009-02-10" version="1">MRKVLVISSLFIILFAFFGCLNLFEKTTTVSVLLTDRPVSDIDKLTVDISSFTYHYAVGDEEGQWATPTNVATTIDILSLAGTEVSWFNIELPENASLTQLRLTIEEATVTIGGEEYPVEVKNKDVKVIKSALNILSGGELVLDFDLARSLHQKGSSNVYILNPVLKPTFRRGKLYFIKGKVLKSGNPLKLSIVALMPTDESTVLRITMTNKYEEFNLGKWKDGEYLIKVYKNVELPDETEDLDLLAFTEDASEIVTVSGEDVFRTINVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGB2</accession>
    <name>B7IGB2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein HflC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hflC</name>
        <name evidence="5" type="ordered locus">THA_648</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">HflC and HflK could regulate a protease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the band 7/mec-2 family. HflC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104486.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_648"/>
    <dbReference type="MEROPS" id="I87.001"/>
    <dbReference type="EnsemblBacteria" id="ACJ75126">
        <property type="protein sequence ID" value="ACJ75126"/>
        <property type="gene ID" value="THA_648"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_648"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059167_1_0_0"/>
    <dbReference type="OMA" id="DFFAFYR"/>
    <dbReference type="OrthoDB" id="1714999at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052547">
        <property type="term" value="P:regulation of peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03405">
        <property type="entry name" value="SPFH_HflC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010200">
        <property type="entry name" value="HflC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_HflKC_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42911">
        <property type="entry name" value="PTHR42911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005651">
        <property type="entry name" value="HflC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01932">
        <property type="entry name" value="hflC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="PHB" evidence="4">
        <location>
            <begin position="21"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR005651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="284" mass="32458" checksum="7F1E749627DDEAFD" modified="2009-02-10" version="1">MKAKIITVSVILLIAIIFLTLSMFVVDQTQQAVVLRFGQIVNTYSTPGIHFRTPFVDNVVKFEKRILLYDIEPEKIITLDKKTLIVDTYALWKIVDAKKFIETMKTIGLAESRIDDIVYSNIRNVFAKHSFDEIISDKRESFLKEVTTLSRADLENFGIEIVDVRVKHADLPSENVNAVYERMKAERYSIAAQIRAEGQKEAQKIRAEADKNVTVILAQAQSQAEKIRGDGEASATRIYALAYQTNPEFFELWRSLSAYDTILNNGTVIFGKDLEIFKYIFEVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGD0</accession>
    <name>RBSD_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-ribose pyranase</fullName>
            <ecNumber evidence="1">5.4.99.62</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rbsD</name>
        <name type="ordered locus">THA_669</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of beta-pyran and beta-furan forms of D-ribose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-ribopyranose = beta-D-ribofuranose</text>
            <dbReference type="Rhea" id="RHEA:25432"/>
            <dbReference type="ChEBI" id="CHEBI:27476"/>
            <dbReference type="ChEBI" id="CHEBI:47002"/>
            <dbReference type="EC" id="5.4.99.62"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodecamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RbsD / FucU family. RbsD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.62" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579713.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IGD0"/>
    <dbReference type="SMR" id="B7IGD0"/>
    <dbReference type="STRING" id="484019.THA_669"/>
    <dbReference type="EnsemblBacteria" id="ACJ75144">
        <property type="protein sequence ID" value="ACJ75144"/>
        <property type="gene ID" value="THA_669"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_669"/>
    <dbReference type="eggNOG" id="COG1869">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135498_0_0_0"/>
    <dbReference type="OMA" id="IIRTGEC"/>
    <dbReference type="OrthoDB" id="1750843at2"/>
    <dbReference type="UniPathway" id="UPA00916">
        <property type="reaction ID" value="UER00888"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062193">
        <property type="term" value="F:D-ribose pyranase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016872">
        <property type="term" value="F:intramolecular lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019303">
        <property type="term" value="P:D-ribose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01661">
        <property type="entry name" value="D_rib_pyranase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023064">
        <property type="entry name" value="D-ribose_pyranase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023750">
        <property type="entry name" value="RbsD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007721">
        <property type="entry name" value="RbsD_FucU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37831">
        <property type="entry name" value="PTHR37831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05025">
        <property type="entry name" value="RbsD_FucU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102546">
        <property type="entry name" value="SSF102546"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000187170" description="D-ribose pyranase">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="117"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01661"/>
        </source>
    </evidence>
    <sequence length="128" mass="14459" checksum="652B88A5E0A46C47" modified="2009-02-10" version="1">MKKTGIFNSEISRVIAKLGHKDKIAIVDLGFPIPSHVERIDIVLDFGRPTFEEVLKVILKELEVEQVILAHESSKKFEQIIKENIPRVEFLKISHEELKKLTNDVVAVIRTGDVVPYSNAILVSGVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGE0</accession>
    <name>B7IGE0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579723.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_682"/>
    <dbReference type="EnsemblBacteria" id="ACJ75154">
        <property type="protein sequence ID" value="ACJ75154"/>
        <property type="gene ID" value="THA_682"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_682"/>
    <dbReference type="eggNOG" id="COG0719">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_894122_0_0_0"/>
    <dbReference type="OMA" id="PVHLCFG"/>
    <dbReference type="OrthoDB" id="635721at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="SSF101960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="315" mass="34841" checksum="624C70C74CF5AB6E" modified="2009-02-10" version="1">MDVKREYEAIVKAVEKLGTDASKFLDKKIASIIISGDKVLGLNNVEGIKLVPTQIENGVQVDMEILENAHIPRPIHVCTGYVEKKGFQKVIFNIKVRKGARVKFLAHCVFPQAEEFEHNAISNVIVEEGAYMEYEDEHYHSDKGTIKLITKTNAVVKEGGVYKNVFSLTKTRVGKLDIVMDLTLEKQAVGELFSKIKASKTDEVNINEILRLEGEHSRGIAKTVVVALDSSKAKVLNEAYGSAPYSKGHISCEEITKGSNVDVSTIPVLKIDNDLSELTHEASIGRVNQKQLEMLMAKGLTEDEATELIIKGLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGE6</accession>
    <name>B7IGE6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Maltose/maltodextrin transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">THA_688</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104557.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_688"/>
    <dbReference type="EnsemblBacteria" id="ACJ75160">
        <property type="protein sequence ID" value="ACJ75160"/>
        <property type="gene ID" value="THA_688"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_688"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_20_1_0"/>
    <dbReference type="OMA" id="FWAKVAV"/>
    <dbReference type="OrthoDB" id="1619224at2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:carbohydrate transport"/>
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    </dbReference>
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        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF4896"/>
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        <property type="entry name" value="MalF-like"/>
    </dbReference>
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        <property type="entry name" value="MalF_N"/>
    </dbReference>
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        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032550">
        <property type="entry name" value="TM_PBP2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47314:SF1">
        <property type="entry name" value="PTHR47314:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16237">
        <property type="entry name" value="DUF4896"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TM_PBP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="535"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="329"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="568" mass="64511" checksum="E873028685DE023B" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGF6</accession>
    <name>B7IGF6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rubrerythrin subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579736.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_699"/>
    <dbReference type="EnsemblBacteria" id="ACJ75170">
        <property type="protein sequence ID" value="ACJ75170"/>
        <property type="gene ID" value="THA_699"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_699"/>
    <dbReference type="eggNOG" id="COG1633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122749_1_0_0"/>
    <dbReference type="OMA" id="YEQDLNP"/>
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    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubrerythrin" evidence="1">
        <location>
            <begin position="5"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="158" mass="18121" checksum="95C6E3C20CB6D5FA" modified="2009-02-10" version="1">MTINELLGVALKIEGAGYSYYEKLSNVSSGALKDLFLELANEEREHARIFEEILSGFKDEKNLNLNEEEVGYLKAFAEDVIFPKLTIEKIPTSVKEAIKLAIEVEKDSIIFYNDIKMLVPDNSAIEKVIEEEKKHMKKLTLRLREDDEFDIYSEGSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGG6</accession>
    <name>B7IGG6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_709</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579744.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_709"/>
    <dbReference type="EnsemblBacteria" id="ACJ75180">
        <property type="protein sequence ID" value="ACJ75180"/>
        <property type="gene ID" value="THA_709"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_709"/>
    <dbReference type="eggNOG" id="ENOG502Z7HP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053973_0_0_0"/>
    <dbReference type="OMA" id="NYFEGWY"/>
    <dbReference type="OrthoDB" id="444212at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009976">
        <property type="term" value="F:tocopherol cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025893">
        <property type="entry name" value="Tocopherol_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35309">
        <property type="entry name" value="PTHR35309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14249">
        <property type="entry name" value="Tocopherol_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="318" mass="36713" checksum="FE78CDCBC91EECA9" modified="2009-02-10" version="1">MGIDNILIYHPESYHGEGKKNFFEGWYFKNTSSNGSYVFAIIPGISIGSDSHSFIQVIDNRGYSKYFRFEFSDFKFSNKPFWVKIKDNYFSLEKIKINLENFEGQLIFKGLKPWPVSIFSPGVMGPFGFLNFLECYHGILSFNHKVRGYLKVNGKEIDFNNGKGYIEKDWGISFPKSWIWSASNDFEKDESISISIATVPFFRNYFVGFIIGLYVRGKLYKFTTYNGSKIKNLEIFEDRIFLTVQKRKYKMNVEIIKSEGNILIAPRQGEMVERISESLNAKVKVKLYKGNELLFAGIGKNAGLEVVGNIIDDMKIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGH1</accession>
    <name>B7IGH1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rluB</name>
        <name evidence="5" type="ordered locus">THA_715</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RsuA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579749.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_715"/>
    <dbReference type="EnsemblBacteria" id="ACJ75185">
        <property type="protein sequence ID" value="ACJ75185"/>
        <property type="gene ID" value="THA_715"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_715"/>
    <dbReference type="eggNOG" id="COG1187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024979_1_2_0"/>
    <dbReference type="OMA" id="SMEFAPF"/>
    <dbReference type="OrthoDB" id="1037615at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042092">
        <property type="entry name" value="PsdUridine_s_RsuA/RluB/E/F_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000748">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB/E/F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018496">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00093">
        <property type="entry name" value="TIGR00093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01149">
        <property type="entry name" value="PSI_RSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="4">
        <location>
            <begin position="8"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="247" mass="28402" checksum="554DFB63C5D2D643" modified="2009-02-10" version="1">MLKGDEIIKLQKYLQMCGFARRKADQYIIAGRVTVNGKVVDKPWFDVKDDDIVMLDNKKLKINQKFEYYVLHKPKGYATTLYDPKQKKTIKELIKHIKTPVKPAGRLDKDATGVLILTNDGKLIDILTNAKYGVKKEYIVKVSGSVSFNDLLKLKKGIYDERDFLKLEYFDILEKGFDYSIIKVVLIKGKKHEIKRLFKSIGHKVLELKRIAHGPISISIVPNEGDLTKLKGKDLERLLKLKKGEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGH7</accession>
    <name>B7IGH7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Shikimate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroK</name>
        <name evidence="1" type="ordered locus">THA_723</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579753.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_723"/>
    <dbReference type="EnsemblBacteria" id="ACJ75191">
        <property type="protein sequence ID" value="ACJ75191"/>
        <property type="gene ID" value="THA_723"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_723"/>
    <dbReference type="eggNOG" id="COG0703">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1659883_0_0_0"/>
    <dbReference type="OMA" id="IYENFHA"/>
    <dbReference type="OrthoDB" id="1392851at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="159" mass="18408" checksum="2C0009ACE4759082" modified="2009-02-10" version="1">MPLYIVGLPGSGKSAVGRILKEDFGYDVVDFDEILKIETGKSFNDIIRNNGLQTIKSLEGKFLKRFKKFEEKIVVTLGTISNLELFNGKIIYIKIPKEKFVKRLKKINKKYSDKELDTIFEEMHSIFSQKADLVISSENKTKFDISKIIDDFYRKSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGJ2</accession>
    <name>B7IGJ2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="3">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">purM</name>
        <name evidence="14" type="ordered locus">THA_740</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AIR synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104694.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_740"/>
    <dbReference type="EnsemblBacteria" id="ACJ75206">
        <property type="protein sequence ID" value="ACJ75206"/>
        <property type="gene ID" value="THA_740"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_740"/>
    <dbReference type="eggNOG" id="COG0150">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047116_0_0_0"/>
    <dbReference type="OMA" id="FNMGYGL"/>
    <dbReference type="OrthoDB" id="923547at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="PTHR10520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="12">
        <location>
            <begin position="50"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="13">
        <location>
            <begin position="163"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ75206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="301" mass="34051" checksum="3152FC4B7B3FCC69" modified="2009-02-10" version="1">MKYTYKQAGVDVKKGDEFAKIIKENVNIPEWILKEPTGYATVLLFTTPPIAVTADGIGTKILLHIEHGTFKEAAKDLIAMNYNDLICVGAKPIAFLDYIGTHKIGEIEKKFIDALIKELKNCDMYLVAGETAEMPDMYQENHFDVAGFAIGTLIKKFEHSHIKPGDYIMALDSSGFHSNGWSLIRKIISEEKINIKDLPFDILKGTRIYKEIIPYFDKIKAMAHVTGGGITRALRRLLGNLGYEINIEKKEFVDWILKYVEFDEAINTFNMGYGLLYVSETDELPGKVVGRVKESERVIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGM8</accession>
    <name>B7IGM8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_780</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579793.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_780"/>
    <dbReference type="EnsemblBacteria" id="ACJ75242">
        <property type="protein sequence ID" value="ACJ75242"/>
        <property type="gene ID" value="THA_780"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_780"/>
    <dbReference type="eggNOG" id="COG3864">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_694047_0_0_0"/>
    <dbReference type="OMA" id="PVFEYAN"/>
    <dbReference type="OrthoDB" id="801007at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025154">
        <property type="entry name" value="Put_metallopeptidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018698">
        <property type="entry name" value="VWA-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09967">
        <property type="entry name" value="DUF2201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13203">
        <property type="entry name" value="DUF2201_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2201_N" evidence="2">
        <location>
            <begin position="4"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2201" evidence="1">
        <location>
            <begin position="259"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="385" mass="44562" checksum="9A952FDC7B0AFA33" modified="2009-02-10" version="1">MVIEKAFQNLMKKSPFYAYLLLGVVLKEDNNVKTMSIKFTKNGEILFLYNKKINKKPIWFVESLILHELMHIINQHFRIKPKNNREKKIWDLAMDAAINQYIPELDARSVPLNVLIQEGHGVDNEYMFAAPPPFMINKTAEEYFDWMMKEFEKKGDFDIEALPDSLDDHNFESDLPIEMIIEITKDKVGKAFNMFGKELESGLKQNINISLQKSTLDWETLIRRFSNASIKGDKYRTPLRPNRRYDDQPGWKYEYNSKLAVIVDTSASIIEEELNQFLTEIEKIAKYDSKLLLIQVDQAVTMVTEYSSGKWKDLEIYGGGETNLQPAVDLAQSHNVEGIIIFTDGHVDVPVVKRRVLFVLSSKHNPDFIEDAVRIYGRSSIVILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGQ7</accession>
    <name>B7IGQ7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zinc protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_809</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579811.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_809"/>
    <dbReference type="EnsemblBacteria" id="ACJ75271">
        <property type="protein sequence ID" value="ACJ75271"/>
        <property type="gene ID" value="THA_809"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_809"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_1_1_0"/>
    <dbReference type="OMA" id="FFEHLLF"/>
    <dbReference type="OrthoDB" id="1188251at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M16" evidence="3">
        <location>
            <begin position="40"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="4">
        <location>
            <begin position="192"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="436" mass="50352" checksum="5DDCEE5D1AE9B375" modified="2009-02-10" version="1">MKRLLLLLMLSIFALFLFAVEIPNVNFVEYTLNNGLKVFIFEDHSVPLVKVEIWYKVGSIDEEEGKTGIAHLLEHTMFNGTNALPKGGIDDLITSVGGSNNAATSYDYTVYYELVPSAKLELALAIEADRMRNLKIDPDDFYREKEVVKQERRMRIENNYIQSGWEELQANAFKGTPLGHFVIGFMEDLERITHIDVRNFYEMFYAPNNAILSISGDVNPEEAIKLVEKYFGEYSPEQVKRPEYAEPKIEGETILKLPRMTRLSLLMELYSIPKADHEDIPAIEALLDIWLNSKNSRVNKELYFNKQLILGTGGFIYDLRIPGMAVIYAFGYKEEDLDAIKDGIDKELERIINEGISEEELQKVKKQMIKSLIFAQKDLKEFSSEIVLGKLRFDNPELYKQKLEKLNQLTSEDIQRVAKKYFYSKNRYVGYIVPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGR4</accession>
    <name>B7IGR4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_816</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100414.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75278">
        <property type="protein sequence ID" value="ACJ75278"/>
        <property type="gene ID" value="THA_816"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_816"/>
    <dbReference type="HOGENOM" id="CLU_204730_0_0_0"/>
    <dbReference type="OMA" id="NVFFISA"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="66" mass="7326" checksum="4ACAB80FF6FA118A" modified="2009-02-10" version="1">MKLISNVFFISAVIFLAGSLIFFEIGMRAMRKKIEEKEKKSTKIAIKLLIVSGILFGISGVLAFFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGX4</accession>
    <name>B7IGX4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_878</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_878"/>
    <dbReference type="EnsemblBacteria" id="ACJ75338">
        <property type="protein sequence ID" value="ACJ75338"/>
        <property type="gene ID" value="THA_878"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_878"/>
    <dbReference type="eggNOG" id="COG0618">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3030956_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="55" mass="6228" checksum="97BE145DBBE2EA8E" modified="2009-02-10" version="1">MNFNALLKFCKGKNILHTTHTKSDCDGIASVFWGLNVFGGEYYIPPHMNLEPLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGX6</accession>
    <name>B7IGX6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycosyl transferase, group 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579855.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_880"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75340">
        <property type="protein sequence ID" value="ACJ75340"/>
        <property type="gene ID" value="THA_880"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_880"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_752130_0_0_0"/>
    <dbReference type="OMA" id="KIIGMES"/>
    <dbReference type="OrthoDB" id="1591999at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="363" mass="43265" checksum="B6ED93E30AE30C52" modified="2009-02-10" version="1">MKILVIGYMHSKEDKRVFRTVETLSRKHKIIYQYLTKDDEKSYTENSITYVPIKYELNKTNYLIEFKNRTNLDKKILKMVENFEYDIIYFHHFLPTRPLKPFKIAKKRGKIIIYDIHEYHPENFLNSLNGMVKVLKEKIMWKIFEKQIMLSNKLVFVSKETQEDIYKKLNVNTPYLIVPNYASISIEPDEKRKEISLVGKITRNIENEKEIIKKLIEKGFKFKIIGMESDAFKDIPHEYTSFLPYEDMMVELSKSAFSLISYNTVKNENYKNDLFALPHKYYDSIAAGTPVIVKNTFLSMTKEVEKYGIGIVINPKNVEESVDKIIKAYENYNILQENIKKYKHLFVWDKDKENEFLNFILKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGZ1</accession>
    <name>B7IGZ1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mannose-1-phosphate guanylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">manC</name>
        <name evidence="2" type="ordered locus">THA_896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579869.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_896"/>
    <dbReference type="EnsemblBacteria" id="ACJ75355">
        <property type="protein sequence ID" value="ACJ75355"/>
        <property type="gene ID" value="THA_896"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_896"/>
    <dbReference type="eggNOG" id="COG0836">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035527_0_1_0"/>
    <dbReference type="OMA" id="ELKKHDP"/>
    <dbReference type="OrthoDB" id="224217at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="368" mass="41943" checksum="39BE1F8234121E20" modified="2009-02-10" version="1">MNNGIIKSVKKAKGGKSMKNCALIMAGGKGERFWPYSTDEKPKQFLNLFNEKTMIQLTVERLKSIIPIENTFVVTGQIYVDLVKEQLPTLPEENIIIEPAGRNTAPCIALSAFYIKEKLGDVNLAVLPADHLVEDVKNFQKALTNAFEFINKNKNAIVTLGIKPDRPETGYGYIKYQSKDNLNINESVLKVERFVEKPSLEVAKKYLSEGNYLWNAGIFVWNTGTILENTKKFLPNTYNILEEIFKYSGKEYLKILGELYPKVDKISVDYGIMEHAKDIYVIPSEFGWDDVGSWTSVERHSKKDENGNVINENTYYFDSKANIVKSKKITILNDVENLVVIETDDYLIISSKESIQKIREIKNKISKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH25</accession>
    <name>ERA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTPase Era</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">era</name>
        <name type="ordered locus">THA_930</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Era GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100821.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IH25"/>
    <dbReference type="SMR" id="B7IH25"/>
    <dbReference type="STRING" id="484019.THA_930"/>
    <dbReference type="PRIDE" id="B7IH25"/>
    <dbReference type="EnsemblBacteria" id="ACJ75389">
        <property type="protein sequence ID" value="ACJ75389"/>
        <property type="gene ID" value="THA_930"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_930"/>
    <dbReference type="eggNOG" id="COG1159">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038009_1_0_0"/>
    <dbReference type="OMA" id="WAEVDVI"/>
    <dbReference type="OrthoDB" id="984717at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04163">
        <property type="entry name" value="Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00367">
        <property type="entry name" value="GTPase_Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030388">
        <property type="entry name" value="G_ERA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005662">
        <property type="entry name" value="GTP-bd_Era"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42698">
        <property type="entry name" value="PTHR42698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00436">
        <property type="entry name" value="era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51713">
        <property type="entry name" value="G_ERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000121363" description="GTPase Era">
        <location>
            <begin position="1"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Era-type G" evidence="2">
        <location>
            <begin position="2"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="1">
        <location>
            <begin position="193"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="2">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="2">
        <location>
            <begin position="36"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="2">
        <location>
            <begin position="57"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="2">
        <location>
            <begin position="119"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="2">
        <location>
            <begin position="149"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="57"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="119"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01050"/>
        </source>
    </evidence>
    <sequence length="299" mass="33721" checksum="361F41A01FA38597" modified="2009-02-10" version="1">MKSGFVALAGKPNVGKSSIVNAIVGKKILIVSDKPQTTRNRINVIHTTDDFQIIFVDTPGIHKPLYRLGEYMVKAAVSALKGVDLILTVVDAKEGVGKPERFVFDYVNQSKTKTIGVINKIDLVDAKKVEQIYNEIKNSLENCVGIVKTSAVRGEGIKELLDLIVENLEEGPQYYPEDMITDRPLSFMASEIIREKIFHHTYEEVPHSVAVIIEEIKERDNGVLYIRANIYVDRNSQKGIIIGQKGNMIKTIGQEARKEIEYLVGGKVFLDLHVKVKKDWRNKDFIILNEIGMRDDLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH28</accession>
    <name>B7IH28_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_933</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579888.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_933"/>
    <dbReference type="EnsemblBacteria" id="ACJ75392">
        <property type="protein sequence ID" value="ACJ75392"/>
        <property type="gene ID" value="THA_933"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_933"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139775_0_0_0"/>
    <dbReference type="OMA" id="NEAVMFF"/>
    <dbReference type="OrthoDB" id="1829044at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009740">
        <property type="term" value="P:gibberellic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="Pentatricopeptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001440">
        <property type="entry name" value="TPR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0939">Gibberellin signaling pathway</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="67"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="139" mass="16316" checksum="DE7F7C4776DCD4C1" modified="2009-02-10" version="1">MEEKLIKAIELSKQGKFDEAEKIYRELIKENVPEAYNNLGNIYRRKGLIAKAIEYYKQALQIKPDFAECYFNMGCAFMEIDNYSLAIFNFEKAEKLGFNDFELDVQLALCYLATGNNRKAQERMKDEKVRQEVLKYIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH39</accession>
    <name>B7IH39_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100852.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_944"/>
    <dbReference type="EnsemblBacteria" id="ACJ75403">
        <property type="protein sequence ID" value="ACJ75403"/>
        <property type="gene ID" value="THA_944"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_944"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_16_0"/>
    <dbReference type="OMA" id="TPEYDIL"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="41"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="167" mass="19888" checksum="A69C36BD129B129C" modified="2009-02-10" version="1">MELNKKLKYLSERDQLTDLYNRHKMNEEIRKHYYNFKRYGRPFSLLMVDIDKFKKINDTYGHMVGDEVLKKLAKILVKNIRKTDIASRWVGEEFLILLSETDLEQAIEVAEKIRKTIEKESFPKDIRITVSIGVDSVENHEHIKDLLILVDEKLYKAKESGRNRVEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH40</accession>
    <name>B7IH40_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Glucose-inhibited division protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">gidA2</name>
        <name evidence="11" type="synonym">gidA</name>
        <name evidence="11" type="synonym">mnmG</name>
        <name evidence="14" type="ordered locus">THA_945</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 11">NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9 11">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the MnmG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579892.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_945"/>
    <dbReference type="EnsemblBacteria" id="ACJ75404">
        <property type="protein sequence ID" value="ACJ75404"/>
        <property type="gene ID" value="THA_945"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_945"/>
    <dbReference type="eggNOG" id="COG0445">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007831_2_2_0"/>
    <dbReference type="OMA" id="FRPGYAI"/>
    <dbReference type="OrthoDB" id="146811at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00129">
        <property type="entry name" value="MnmG_GidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="PTHR11806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PTHR11806:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00136">
        <property type="entry name" value="gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01280">
        <property type="entry name" value="GIDA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01281">
        <property type="entry name" value="GIDA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 11" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="GIDA" evidence="12">
        <location>
            <begin position="13"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="GIDA_C" evidence="13">
        <location>
            <begin position="407"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="18"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="280"/>
            <end position="294"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ75404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="624" mass="70320" checksum="76DC9A33FDEE5108" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH56</accession>
    <name>B7IH56_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_961</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100919.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_961"/>
    <dbReference type="EnsemblBacteria" id="ACJ75420">
        <property type="protein sequence ID" value="ACJ75420"/>
        <property type="gene ID" value="THA_961"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_961"/>
    <dbReference type="HOGENOM" id="CLU_1626269_0_0_0"/>
    <dbReference type="OMA" id="VEWYIFL"/>
    <dbReference type="OrthoDB" id="1963260at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="163" mass="18685" checksum="93A357D64A95CC9E" modified="2009-02-10" version="1">MICNRNIGGVNISFSEVLVGIIIAVIVLASCCMMLNSVINWYFNIKGDYLRTLEAFFIVNYIRHDFYSRGISEIVYISPQSISFKERIKTGAIKSVRYKVLDYNGKCVVERQASDGKNYFGPFNEKIEFEDGNGYILIKSYIGKFILPKKPVDKVIRKLIPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH85</accession>
    <name>B7IH85_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75449">
        <property type="protein sequence ID" value="ACJ75449"/>
        <property type="gene ID" value="THA_990"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_990"/>
    <dbReference type="HOGENOM" id="CLU_3067156_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="53" mass="5842" checksum="694D4CF043030B67" modified="2009-02-10" version="1">MSSQSILMIAQQIEEMMEAVKQLAYASQDISVSSQELTALANNLVEQVKTLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH98</accession>
    <name>B7IH98_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579923.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1003"/>
    <dbReference type="EnsemblBacteria" id="ACJ75462">
        <property type="protein sequence ID" value="ACJ75462"/>
        <property type="gene ID" value="THA_1003"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1003"/>
    <dbReference type="eggNOG" id="COG1031">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_533842_0_0_0"/>
    <dbReference type="OMA" id="HTPGKYL"/>
    <dbReference type="OrthoDB" id="376425at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="153"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="518" mass="58543" checksum="F6144F0B48BC1707" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHB0</accession>
    <name>B7IHB0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methyl-accepting chemotaxis protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1016</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579934.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1016"/>
    <dbReference type="EnsemblBacteria" id="ACJ75474">
        <property type="protein sequence ID" value="ACJ75474"/>
        <property type="gene ID" value="THA_1016"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1016"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_19_0"/>
    <dbReference type="OMA" id="YMYYSYA"/>
    <dbReference type="OrthoDB" id="477199at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="6">
        <location>
            <begin position="307"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="379"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="373"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="604"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="665" mass="72882" checksum="EFCC796545A3D768" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHB5</accession>
    <name>B7IHB5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101045.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1021"/>
    <dbReference type="EnsemblBacteria" id="ACJ75479">
        <property type="protein sequence ID" value="ACJ75479"/>
        <property type="gene ID" value="THA_1021"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1021"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_7_2_0"/>
    <dbReference type="OMA" id="PAYQLGW"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBP_bac_5" evidence="1">
        <location>
            <begin position="65"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="500" mass="57071" checksum="C94FE1AB38EBFF59" modified="2009-02-10" version="1">MKKFMIIIGLLLVVFSFASKDVIVVGTTDKIRTLDPANCYDYFSSNILQNVLSGPVDYEIGTANIKPWLFESWDVSDDGLVYTFHIRKDAYFEDGTQIDANVFKYSFDRVMKLNGDPAFLLTDVVEKTEVVDKFTFRVTLKSKFSAFVSVLGYTVAFPVNPKVFPEDNFFEGAPSASGPYKITEWIRDIRIVLEENPKYFGPKPKTPKIIIQFYENAQQLRLALETGEVDIAYRHLDPRDILDLKNVPGIKVYLGASPQIRYLVVNVKQKPFDNPLVRQAIALAVDRETIVNDIFVGLAKPLYSMVPAGMWSHEDVFPKRDLKAAVEILKVAGYTKDNPLVIDLWYTPSHYGTTEADVAQVLKESLEQTGVIKVNIKYAEWSTYVEYFLNGTMGLFLLGWYPDYLDPDDYLWPFLSINGAKSLGSFYENKNVEDLMVAARAATDQFSREMIYKLVQNFHAAEVPYIPLWQGSADCEAHDYIDGIILEPTQIFRYYILERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHC5</accession>
    <name>B7IHC5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101064.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1031"/>
    <dbReference type="EnsemblBacteria" id="ACJ75489">
        <property type="protein sequence ID" value="ACJ75489"/>
        <property type="gene ID" value="THA_1031"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1031"/>
    <dbReference type="HOGENOM" id="CLU_2290343_0_0_0"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="101" mass="12107" checksum="A1BA61303628E3BC" modified="2009-02-10" version="1">MYSDVKVDLWEMEKILSNFDRSEKLLEKILDLYTGPFLAGIECLWAKQMRRYYNVLLKSIVDDYLENIGFESRLAVKLMKVFPDLSIFDEELESEYLYDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHE8</accession>
    <name>B7IHE8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Prepilin-type N-cleavage/methylation domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1056"/>
    <dbReference type="EnsemblBacteria" id="ACJ75512">
        <property type="protein sequence ID" value="ACJ75512"/>
        <property type="gene ID" value="THA_1056"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1056"/>
    <dbReference type="eggNOG" id="COG4969">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149241_0_0_0"/>
    <dbReference type="OMA" id="ENYFNTE"/>
    <dbReference type="OrthoDB" id="2048191at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045584">
        <property type="entry name" value="Pilin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54523">
        <property type="entry name" value="SSF54523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00409">
        <property type="entry name" value="PROKAR_NTER_METHYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="141" mass="15699" checksum="F0D14268B30129C1" modified="2009-02-10" version="1">MKMKKGFTLVELLIVLAVIAALLSVATPLALNAVKKAKASQVAQNFRAIKSAVENWFNTESPADPKTEFSKDTLLKKNYINTWPDGFEVTPEASNTGVYEFTIKYTKGDISLVDVEKSGMPEVDEGENNNLVLKFALQKWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHF1</accession>
    <name>B7IHF1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Beta-N-acetylglucosaminidase CbsA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1059</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579966.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1059"/>
    <dbReference type="CAZy" id="GH3">
        <property type="family name" value="Glycoside Hydrolase Family 3"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75515">
        <property type="protein sequence ID" value="ACJ75515"/>
        <property type="gene ID" value="THA_1059"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1059"/>
    <dbReference type="eggNOG" id="COG1472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008392_0_1_0"/>
    <dbReference type="OMA" id="WQMAAEM"/>
    <dbReference type="OrthoDB" id="949956at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_3" evidence="2">
        <location>
            <begin position="15"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="444" mass="51312" checksum="0F86C73804653F01" modified="2009-02-10" version="1">MNDFGNLFFIGFQNQFEKDFLERINPCGIILYPENMNDVYKLQINMERIYSLFDKGYRFFISSDHEGGQLETVPNIFPSPGNMAIGKVGSASEFGNYLAHMLKLHGFNMVFAPVVDVKHEKSSHVTGFRAYSDDHEIIKMRALEFIRSLLDENIAPTLKHFPGHGKAKEDSHFTLPVVFDFDENDEDMMIFKELSKHVDFIMTAHVMYPKLDEVVATLSKKILKGILREKFGYKGLIISDAFEMKALYKNYSPKEVIKRFFEADGDIILIGDVENHYYLIETFNSMVKSGELDIELLKEKVEKIKKIKEKYVSDTYPTRFLSKIAKQAIDFKLDSPVENPSFLIPQAKNLSLADTSQKDLKYLRNLIKEEFKDAKIYTSSSEISNEDTVIYFVLDKVENVNAKRVIYIFLRDVLKKDVEYVNPYSSKLISIYHVLQLFKGEGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHK0</accession>
    <name>B7IHK0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Methyl-accepting chemotaxis protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1111</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1111"/>
    <dbReference type="EnsemblBacteria" id="ACJ75564">
        <property type="protein sequence ID" value="ACJ75564"/>
        <property type="gene ID" value="THA_1111"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1111"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_590426_0_0_0"/>
    <dbReference type="OMA" id="NTTIMIF"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033479">
        <property type="entry name" value="dCache_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029151">
        <property type="entry name" value="Sensor-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02743">
        <property type="entry name" value="dCache_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103190">
        <property type="entry name" value="SSF103190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="311"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="376"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="463" mass="50610" checksum="5134CC79CACBC2AE" modified="2009-02-10" version="1">MKSIKGKLLIFVLMPVIALLIISTFLISFQTRKSVEDLSIETALEISQKASQIIEEWLNGILRDVQNFADTSSVIEALKTRNWKDLMSKELNYKLSSRTDYEMFFIAYPDGTAPTTSGVTANVSDREYFQKIIKQGYKSYISDALISKATGNGIFIVAAAVKDENGKTIGLFGAAVKLDDLNKIAVETKVGDKGFGWIIDSTGLTIAHPDEKIRMKVNILEASKQGFKGLDTIAQKMLEGKSGYGSYTRSDGSEVYVFYSPIKVGKGWSFGLTLYKSELFSSVNKITNTTIMIFLSVIILISIFIYISSSYFISKPLTAMVKNILEFGKGNLTVKFIAKGKDEIAQISNSLQKMADMLRESFSQINETSLNLNSSSEELANIADNLSASAQELSAQITEIDNSIQNASASVEEVTSGVEEVAASAQNVSNAAQNISDNANKMAENAKEGEKEVVAISEIVEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHP6</accession>
    <name>B7IHP6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580046.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1158"/>
    <dbReference type="EnsemblBacteria" id="ACJ75610">
        <property type="protein sequence ID" value="ACJ75610"/>
        <property type="gene ID" value="THA_1158"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1158"/>
    <dbReference type="eggNOG" id="COG1011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_9_1_0"/>
    <dbReference type="OMA" id="NVCTHDW"/>
    <dbReference type="OrthoDB" id="1669868at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="201" mass="24046" checksum="23A5FB14298EFBDD" modified="2009-02-10" version="1">MINNIIFDLGRVLINWEPEKYMKKVFDEKTISFLLEKTFNTNDWNLMDKGVIDEQTLWENKLNQYPEYKKEIEHMKNKVLDLLTPIEENAKLLYILKEKGYNLYILSNFSKIAFQKVYEKYDFFKLFDGMIISSHVKSIKPEDEIYKILIEKYKINPAESLYIDDKIENIKAGEKFGFKTIHLEKPEDLKDKLSKILKIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHQ0</accession>
    <name>B7IHQ0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Threonine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1162</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580050.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1162"/>
    <dbReference type="EnsemblBacteria" id="ACJ75614">
        <property type="protein sequence ID" value="ACJ75614"/>
        <property type="gene ID" value="THA_1162"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1162"/>
    <dbReference type="eggNOG" id="COG0031">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_577286_0_0_0"/>
    <dbReference type="OMA" id="LKCIIVQ"/>
    <dbReference type="OrthoDB" id="428821at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PALP" evidence="3">
        <location>
            <begin position="58"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="475" mass="52673" checksum="BB61338C82F67F01" modified="2009-02-10" version="1">MRDLSYDAAMSRKNEIMKKAVGIDYEKFISSEIVFDYERMMQEVGYSLDKVREIQSETGVGNTPLVELKQINRLIKKIAPKGKGARIFIKDEATNPSGSYKDRRASVSVYHAQKHGYKGVIAATSGNYGAAVASQAAKRGLKCIIAQECYDSRWIGQPEILEKARSCEAYGAEVVQLTVGPELFYYTLVLLEETGFFNASLYTPYAIAGVETLGYEIAEQTMQMVGKYPDAVVITHAGGGILTGTARGLKKAGANETEIIAASVNLRGLHMASDNDFNKKYFTTGHTGFGIPFATFPDRSDVPKNAARALRYMDRYLLVTQGEVFYITEMLAQLEGMQRGPAGNTSLAAAFALALEMDDDKVIVVNETEYTGAGKLPSAQLTFARKMGMEIKRGDPIKEDLPGKRIVIPEHPSQIGYIEFDMDEVRKSYVKEVIKRYNKKEFNKQEIEFMAQDTNTSIEKIVDFVKELGGEVIEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHU5</accession>
    <name>RS10_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsJ</name>
        <name type="ordered locus">THA_1214</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101436.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHU5"/>
    <dbReference type="SMR" id="B7IHU5"/>
    <dbReference type="STRING" id="484019.THA_1214"/>
    <dbReference type="PRIDE" id="B7IHU5"/>
    <dbReference type="EnsemblBacteria" id="ACJ75659">
        <property type="protein sequence ID" value="ACJ75659"/>
        <property type="gene ID" value="THA_1214"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1214"/>
    <dbReference type="eggNOG" id="COG0051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122625_1_3_0"/>
    <dbReference type="OMA" id="QFEIRVH"/>
    <dbReference type="OrthoDB" id="2012682at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_S10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_S10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_S10_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="PTHR11700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="SSF54999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01049">
        <property type="entry name" value="rpsJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000127194" description="30S ribosomal protein S10">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <sequence length="102" mass="11686" checksum="B7F260EA80993E54" modified="2009-02-10" version="1">MPGQKIRIRLKAYDHKLLDESAKKIVEIVKQTNAKVSGPIPLPTERTLYVVLRSPLKHKDSREQFEKRIHKRLIDILEPSPKTIDALMKINLPAGVDVEINL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHV0</accession>
    <name>RS19_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsS</name>
        <name type="ordered locus">THA_1219</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101445.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHV0"/>
    <dbReference type="SMR" id="B7IHV0"/>
    <dbReference type="STRING" id="484019.THA_1219"/>
    <dbReference type="EnsemblBacteria" id="ACJ75664">
        <property type="protein sequence ID" value="ACJ75664"/>
        <property type="gene ID" value="THA_1219"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1219"/>
    <dbReference type="eggNOG" id="COG0185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144911_0_1_0"/>
    <dbReference type="OMA" id="FPEMVGH"/>
    <dbReference type="OrthoDB" id="1893906at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_S19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005732">
        <property type="entry name" value="Ribosomal_S19_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_S19_S15_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="PTHR11880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="SSF54570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01050">
        <property type="entry name" value="rpsS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000128047" description="30S ribosomal protein S19">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <sequence length="95" mass="10681" checksum="3BC35926D6A900AC" modified="2009-02-10" version="1">MGRSTKKGPYVDPKLLKKIRQLNEAGEKKIIKTWSRASMIVPEMVGHTIAVYNGLKHIPVYITENMVGHRLGEFSFTRRFGGHADKSASKGQVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHW8</accession>
    <name>B7IHW8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Methionine aminopeptidase</fullName>
            <shortName evidence="2">MAP</shortName>
            <shortName evidence="2">MetAP</shortName>
            <ecNumber evidence="2 3">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">map</name>
        <name evidence="5" type="ordered locus">THA_1237</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2 3">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="2 3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101478.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1237"/>
    <dbReference type="EnsemblBacteria" id="ACJ75682">
        <property type="protein sequence ID" value="ACJ75682"/>
        <property type="gene ID" value="THA_1237"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1237"/>
    <dbReference type="eggNOG" id="COG0024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_0_1_0"/>
    <dbReference type="OMA" id="GDHAYTF"/>
    <dbReference type="OrthoDB" id="1285136at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070084">
        <property type="term" value="P:protein initiator methionine removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M24" evidence="4">
        <location>
            <begin position="11"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="255" mass="28126" checksum="29CC53271D9D1515" modified="2009-02-10" version="1">MIYIKSKEEIEKIKIASQAVGTILSEAKKVAVEGASAWDIELLAEKILKELKCAPAFKGYNGYPYITTVSVNDEVIHGFPLKKKVFKKGDIVSIDVGAIYEGYYGDGAYTYIIGETDEIGTKLVEVTKKSLEIAIETVRAGIRLGDVSYEIQKYVEENGFNVVRDFVGHGVGKKLHEDPQIPNYGKKGTGVILREGMTIAIEPMVTEGGWHVVILDDGWTVVTKDGKRAAHFEHTLLVKKDGCEVLTRRGDGFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHX6</accession>
    <name>B7IHX6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">ATP-dependent Clp protease proteolytic subunit</fullName>
            <ecNumber evidence="5 8">3.4.21.92</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Endopeptidase Clp</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 11" type="primary">clpP</name>
        <name evidence="11" type="ordered locus">THA_1245</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 9">Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 10">Belongs to the peptidase S14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.92" evidence="5 8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101502.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1245"/>
    <dbReference type="MEROPS" id="S14.001"/>
    <dbReference type="EnsemblBacteria" id="ACJ75690">
        <property type="protein sequence ID" value="ACJ75690"/>
        <property type="gene ID" value="THA_1245"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1245"/>
    <dbReference type="eggNOG" id="COG0740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058707_3_2_0"/>
    <dbReference type="OMA" id="RDYWMKA"/>
    <dbReference type="OrthoDB" id="1728970at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018215">
        <property type="entry name" value="ClpP_Ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="PTHR10381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00493">
        <property type="entry name" value="clpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00381">
        <property type="entry name" value="CLP_PROTEASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 8" id="KW-0720">Serine protease</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 7">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="201" mass="22407" checksum="A75E11E2332C5E2C" modified="2009-02-10" version="1">MKEIINQYVPMVVESNGKYERAYDIYSRLLKDRIVFLGSAIDDHVANLVVAQLLFLEAEDPDKDIQLYINSPGGSVSAGLAIYDTMQYIKCDVATICIGQAASMGAVLLAGGTKGKRFSLPNSRIMIHQPLGGAEGPAKDVEIMTKELLRIKNKINEILSFHTGQPIERIEKDTDRDFFMTPEEALEYGLIDKVIKPEDRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHX7</accession>
    <name>Y1246_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable transcriptional regulatory protein THA_1246</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">THA_1246</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TACO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580086.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHX7"/>
    <dbReference type="SMR" id="B7IHX7"/>
    <dbReference type="STRING" id="484019.THA_1246"/>
    <dbReference type="EnsemblBacteria" id="ACJ75691">
        <property type="protein sequence ID" value="ACJ75691"/>
        <property type="gene ID" value="THA_1246"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1246"/>
    <dbReference type="eggNOG" id="COG0217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062974_2_2_0"/>
    <dbReference type="OMA" id="ETIMYEG"/>
    <dbReference type="OrthoDB" id="1304328at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00693">
        <property type="entry name" value="Transcrip_reg_TACO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017856">
        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002876">
        <property type="entry name" value="Transcrip_reg_TACO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026564">
        <property type="entry name" value="Transcrip_reg_TACO1-like_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532">
        <property type="entry name" value="PTHR12532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01709">
        <property type="entry name" value="Transcrip_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="SSF75625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01033">
        <property type="entry name" value="TIGR01033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_1000132246" description="Probable transcriptional regulatory protein THA_1246">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00693"/>
        </source>
    </evidence>
    <sequence length="252" mass="27904" checksum="39230B4772F98A39" modified="2009-02-10" version="1">MSGHNKWANIKHRKAAQDAKKSKIFTKLIREIIVAAKEGGGDPETNPRLRAVLERARAANMPKDTIEKSIKKGTGELEGVDYQEIIYEAYAPAGVALYIYAMTDNKNRTAQELRHLLSKHGGSLAESGSVAWLFERKGIIEIPKEKIADFEEFAMVAIDAGAEDIIEDDPIQVITAPDMLTEVKDKLAENGFEGEAKVTFIPKNTVKVTGADAEKVLKLVSVLEDNDDVQEVYANFEIDDKELEEIMSKLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHX9</accession>
    <name>B7IHX9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-glutamate O-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1248</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="2.1.1.80"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.80" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1248"/>
    <dbReference type="EnsemblBacteria" id="ACJ75693">
        <property type="protein sequence ID" value="ACJ75693"/>
        <property type="gene ID" value="THA_1248"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1248"/>
    <dbReference type="eggNOG" id="COG1352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025854_1_1_0"/>
    <dbReference type="OMA" id="EWQEFVN"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008983">
        <property type="term" value="F:protein-glutamate O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.155.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026024">
        <property type="entry name" value="Chemotaxis_MeTrfase_CheR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022642">
        <property type="entry name" value="CheR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000780">
        <property type="entry name" value="CheR_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022641">
        <property type="entry name" value="CheR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036804">
        <property type="entry name" value="CheR_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01739">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03705">
        <property type="entry name" value="CheR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000410">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00996">
        <property type="entry name" value="CHERMTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00138">
        <property type="entry name" value="MeTrc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50123">
        <property type="entry name" value="CHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CheR-type methyltransferase" evidence="6">
        <location>
            <begin position="4"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="270" mass="32246" checksum="EE491E67825F7F45" modified="2009-02-10" version="1">MNSLSGNTQEFSMEEFNWFLEVVKKDFNFDLTGYKPHRVKRRIEMLIRKYGLKSYKEYYELIKKDEKKKDEFFDRMTINVTEFFRNPEKWWELRDKFIPQLLKESGSKFKAWSAGCSTGEEPYSLAILLEELKAPSSAKVHATDIDIGVLTKARMGEYEERSFVSTPPQYLKKYFEVTSRGTYRVKQNVKNRVIFKRHNLLQDRFETGYDLILCRNVVIYFEMETKMELYEKFAKALRPGGILFIGNTERIFNYRQLGFEVASPFIYRKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICK4</accession>
    <name>Y1286_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0597 protein THA_1286</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">THA_1286</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0597 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101568.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ICK4"/>
    <dbReference type="SMR" id="B7ICK4"/>
    <dbReference type="STRING" id="484019.THA_1286"/>
    <dbReference type="EnsemblBacteria" id="ACJ75731">
        <property type="protein sequence ID" value="ACJ75731"/>
        <property type="gene ID" value="THA_1286"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1286"/>
    <dbReference type="eggNOG" id="COG3681">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051840_0_0_0"/>
    <dbReference type="OMA" id="GAMIPVM"/>
    <dbReference type="OrthoDB" id="1326279at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01845">
        <property type="entry name" value="UPF0597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005130">
        <property type="entry name" value="Ser_deHydtase-like_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021144">
        <property type="entry name" value="UPF0597"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30501">
        <property type="entry name" value="PTHR30501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03313">
        <property type="entry name" value="SDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006054">
        <property type="entry name" value="UCP006054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000188474" description="UPF0597 protein THA_1286">
        <location>
            <begin position="1"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01845"/>
        </source>
    </evidence>
    <sequence length="402" mass="43596" checksum="18D1E9DB78681B94" modified="2009-02-10" version="1">MIKDIFFDQVKPAYGCTEPIAVALSTATAKKYLNETLEDLKEINITLDKNTYKNGLVVNIPGTNIFGLEIAAALGYLCGIPEKGLEVLKDVNSQCLSKATNLKNKIKIEINDMPYLYVETIAVSKDNHTVKILIEGKHDNIAKITIDSNTILDKPFNPSKTTLENIKKYSIDEIIDYVENPDKEVLDYVEKAIEMNIDIAKYGMQMKGNFSNAAINEYVKYVSAGVDARMSGVLKPVMTVAGSGNQGLSCILPIATKREEYDKEKILKATLLSILVTIYIKAYTGLLTPICGAGSISAAGSAAGLTYLKGGNRQQIKNAINDTIGTLFGLTCDGAKRGCALKAVTGTLTAIQVSELAINNIDVPCGNGIVAKDVEETIRRVGKLTNSVKQFDKDVLDYIGKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICK9</accession>
    <name>B7ICK9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1291</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580124.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1291"/>
    <dbReference type="EnsemblBacteria" id="ACJ75736">
        <property type="protein sequence ID" value="ACJ75736"/>
        <property type="gene ID" value="THA_1291"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1291"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_15_0_0"/>
    <dbReference type="OMA" id="DYIFPRI"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025997">
        <property type="entry name" value="SBP_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13407">
        <property type="entry name" value="Peripla_BP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="350" mass="39403" checksum="1AEBC5495C4BC03D" modified="2009-02-10" version="1">MIPLYLKIANYLETKILKGEYLPGDKIPSENILSEQFNTTRTTVRKALAELEKKGIITRVFGLGTFVTEINIVSKKKIGIIVQNTKIVYGIIKFCSKIGSKCFVVERVENLEAEKQAIKQLLSMGVDGIIMEPTVNSIANDILKSLLQNNFPVVFVDRSIDIGFPVPTVINNNYSGGKMLGEHMRKVHKIKSAIYVTSEDMTISSVYERYNGIKDGLKFSPEIIKVPKIDGDYSQIIKFAKKVDAIFFCNDMMAVRGITTLLQNNIKIPNDIKVLGYDNDYVSKIVNPKLTTVNQDLSLIGETAASLMVSLIKNEKVELENKIESKLIIRKSCGCKEEEEKNVQRKRQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICP6</accession>
    <name>B7ICP6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA pseudouridine synthase B</fullName>
            <ecNumber evidence="5">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">tRNA pseudouridine(55) synthase</fullName>
            <shortName evidence="5">Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">truB</name>
        <name evidence="8" type="ordered locus">THA_1328</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580148.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1328"/>
    <dbReference type="EnsemblBacteria" id="ACJ75773">
        <property type="protein sequence ID" value="ACJ75773"/>
        <property type="gene ID" value="THA_1328"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1328"/>
    <dbReference type="eggNOG" id="COG0130">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032087_0_2_0"/>
    <dbReference type="OMA" id="ELQFIRW"/>
    <dbReference type="OrthoDB" id="1166299at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02573">
        <property type="entry name" value="PseudoU_synth_EcTruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01080">
        <property type="entry name" value="TruB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="PTHR13767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00431">
        <property type="entry name" value="TruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TruB_N" evidence="6">
        <location>
            <begin position="23"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="TruB_C_2" evidence="7">
        <location>
            <begin position="171"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="306" mass="34702" checksum="5019C90163CA6F06" modified="2009-02-10" version="1">MVGFLNAFKPKGVTSHDVIDELRKKLKTKKIGHAGTLDPFAEGVLVIGISGATRLLEYLKNEKKRYYVKAILGLITETFDITGQVKEERVCQKSNDEIKNAILAFKGKYKQVPPAYSAKKYNGERLYKLAREGKIISLPPVEVEIYEIENIKIVPPEFSFEVVVSPGTYIRSLCMDIGYKLGCGATAVELVRKSVGKFSIKDSINPFELSSEQIVNRILRVEEVLDLPQVEIYKDYVEKIFNGVQPTMDFVKEIKDDFRKDQDVMLVCNGRLVAIAVSQRTSNFLKKNEVRGTVFKLKKVFREGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICQ9</accession>
    <name>B7ICQ9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sucrose-phosphate synthase</fullName>
            <ecNumber evidence="3">2.4.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1341</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 6-phosphate + UDP-alpha-D-glucose = H(+) + sucrose 6(F)-phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:22172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:57723"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.14" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580159.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1341"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75786">
        <property type="protein sequence ID" value="ACJ75786"/>
        <property type="gene ID" value="THA_1341"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1341"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_24_2_0"/>
    <dbReference type="OMA" id="VAYPKKH"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046524">
        <property type="term" value="F:sucrose-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044161">
        <property type="entry name" value="SPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46039">
        <property type="entry name" value="PTHR46039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="5">
        <location>
            <begin position="26"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="4">
        <location>
            <begin position="251"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="468" mass="53742" checksum="A69855B07137252A" modified="2009-02-10" version="1">MKKIAFFNPQGNFDKNDSHLTEHPDFGGQLVYVKELAKAIASKGIQVDIITRQIIDESWPEFSEPFDYYPDSPNLRIVRIPFGGKKFLNKEKLWKYLPEYVEGIYSLYKKEGSFPDFVTTHYGDGGISGVLFLEKTDIPFSFTGHSLGAWKLEKMLNEGFSQEELERKFKFSVRILAENLAIKYSSFVVCSTSHERYVQYSHKLYNADPNSDKFKVIPPGINSKIFNLKPHKEDEIIENYLNNVLSSAPKERQKLPFIILSSRIDRKKNHIAVVRAFLKNKDLKEIANLIIVVRAIDDVIKFVNEKDSEESEILREIINEGKKEIGNSIFFLNISDQKSLASLYRVAAKRNSVFTLPSHYEPFGLAIIEAAACGLVVSATKYGGPIEILSDNKELLFDPENVDDIANKLYIALTKYNNSQFIELSRKYTWESTANKYLENIDKVLKNKLEINKMQLQDDIKKFLSKIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICR5</accession>
    <name>RL10_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplJ</name>
        <name type="ordered locus">THA_1347</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the ribosomal stalk of the 50S ribosomal subunit. The N-terminus interacts with L11 and the large rRNA to form the base of the stalk. The C-terminus forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580164.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ICR5"/>
    <dbReference type="SMR" id="B7ICR5"/>
    <dbReference type="STRING" id="484019.THA_1347"/>
    <dbReference type="EnsemblBacteria" id="ACJ75792">
        <property type="protein sequence ID" value="ACJ75792"/>
        <property type="gene ID" value="THA_1347"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1347"/>
    <dbReference type="eggNOG" id="COG0244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092227_1_2_0"/>
    <dbReference type="OMA" id="VRDQKQA"/>
    <dbReference type="OrthoDB" id="1470978at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05797">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00362">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022973">
        <property type="entry name" value="Ribosomal_L10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_L10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002363">
        <property type="entry name" value="Ribosomal_L10_eubac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_L10P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11560">
        <property type="entry name" value="PTHR11560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="SSF160369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01109">
        <property type="entry name" value="RIBOSOMAL_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000121025" description="50S ribosomal protein L10">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00362"/>
        </source>
    </evidence>
    <sequence length="180" mass="20038" checksum="677BC07F6D1D08E6" modified="2009-02-10" version="1">MLTRAQKEQLVKELSEVFKNSSLILFSDYKGLNVAQITDLRKKLREKLADGARYKVIKNSVAYLALKEAGYNVEEIEDVFSGPLAILYVEDGDPIEAIKVIYDFSKEMKGIPSFKGLYLDGKFFSADEVENLSKLPSKEQLLAMVVSGVQGPIRGLVNVLSGTLKNLLYALNAIKDKKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICS1</accession>
    <name>B7ICS1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Replicative DNA helicase</fullName>
            <ecNumber evidence="3 12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">dnaB2</name>
        <name evidence="14" type="ordered locus">THA_1353</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the helicase family. DnaB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 12"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580170.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1353"/>
    <dbReference type="EnsemblBacteria" id="ACJ75798">
        <property type="protein sequence ID" value="ACJ75798"/>
        <property type="gene ID" value="THA_1353"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1353"/>
    <dbReference type="eggNOG" id="COG0305">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005373_0_0_0"/>
    <dbReference type="OMA" id="WENLAKC"/>
    <dbReference type="OrthoDB" id="1709134at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00984">
        <property type="entry name" value="DnaB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036185">
        <property type="entry name" value="DNA_heli_DnaB-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007692">
        <property type="entry name" value="DNA_helicase_DnaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007694">
        <property type="entry name" value="DNA_helicase_DnaB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007693">
        <property type="entry name" value="DNA_helicase_DnaB-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016136">
        <property type="entry name" value="DNA_helicase_N/primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00772">
        <property type="entry name" value="DnaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03796">
        <property type="entry name" value="DnaB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48024">
        <property type="entry name" value="SSF48024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00665">
        <property type="entry name" value="DnaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51199">
        <property type="entry name" value="SF4_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 12 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="8 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 12" id="KW-0639">Primosome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SF4 helicase" evidence="13">
        <location>
            <begin position="177"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ75798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="445" mass="50213" checksum="B168F6048B921138" modified="2009-02-10" version="1">MKNAPHSIEAEQAILGSILIDPETIENIISIVSSRDFFDPRHAEIFKAIEELYDEGIPIDVISICDRLKTKGKLEMVGGEIYVAQLADIVPTSAHAEMYAQIVRDKSVLRALINAASKVVEDAMSDRDVDDILDNAERVIFEIAESKTSKTYLSMDTILHEVFENLENLRTKVKDGVTSLITGIPTGFKKLDEMTSGFHKSDLIILAARPSVGKTAFALNIAKNMALHAKVPVGIFSLEMSKEQLAQRLLGMEALIELQKIRRGTLSDEEWQRLLTATGKLYKANIIVDDEANLDPRSLRAKARRMKKEYGIEAIFIDYLQLMSIKSYRENRQQEISEISRSLKLLARELDVSIIALSQLSRAVEQREDKRPRLSDLRESGSIEQDADMVLFLYREQYYKKEKTLDPHETEVIIGKQRNGPIGTVTLLFDPKFTAFFEIDPTHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICU1</accession>
    <name>B7ICU1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8 10">FAD:protein FMN transferase</fullName>
            <ecNumber evidence="2 8 10">2.7.1.180</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Flavin transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">THA_1373</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Flavin transferase that catalyzes the transfer of the FMN moiety of FAD and its covalent binding to the hydroxyl group of a threonine residue in a target flavoprotein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 10">
            <text>FAD + L-threonyl-[protein] = AMP + FMN-L-threonyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36847"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:74257"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.1.180"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Magnesium. Can also use manganese.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the ApbE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.180" evidence="2 8 10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580188.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1373"/>
    <dbReference type="EnsemblBacteria" id="ACJ75818">
        <property type="protein sequence ID" value="ACJ75818"/>
        <property type="gene ID" value="THA_1373"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1373"/>
    <dbReference type="eggNOG" id="COG1477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044403_1_2_0"/>
    <dbReference type="OMA" id="MGTYYSI"/>
    <dbReference type="OrthoDB" id="2021063at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017013">
        <property type="term" value="P:protein flavinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024932">
        <property type="entry name" value="ApbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30040">
        <property type="entry name" value="PTHR30040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02424">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006268">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 8 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006268-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ75818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="352" mass="39892" checksum="486435FA7FC84ED4" modified="2009-02-10" version="1">MRKKSSGDINKLTKLSLVSTVLILVIVAIILIVAVFFKPNMKYETFETFSLGTYVQLRISTSVNPSVLSKEIFNELDRITKKFDAYNEQSIIYKINHSNDWVEVDDETFAILDLSLQYAKKTNFAFDPTLGRVIDLWGFSKFSEKEASSLVVPRKEDIEEALKLSGAKLVEIDYKKKAVKTNGVWFDLGGIVKGYALERAYQIAKQADPNCTGFIEAGGDIRILGPKFGKEYWIIGIRNPRGNDSIDYIYLKSGAVATSGDYERYFIVDGKRYHHLIDSKTGYPADSAISATVVSDDAIKADVFSTAAFVFGKKDWIYTRTIIPKYDSEVFLVTPEFEYLKTDGFEYYEKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICU6</accession>
    <name>B7ICU6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ErfK/YbiS/YcfS/YnhG family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1378</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1378"/>
    <dbReference type="EnsemblBacteria" id="ACJ75823">
        <property type="protein sequence ID" value="ACJ75823"/>
        <property type="gene ID" value="THA_1378"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1378"/>
    <dbReference type="eggNOG" id="COG1376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_723471_0_0_0"/>
    <dbReference type="OMA" id="NPTYGIH"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="SSF54106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="LysM" evidence="3">
        <location>
            <begin position="174"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="379" mass="42850" checksum="9BB3AA730FFC9AF5" modified="2009-02-10" version="1">MIVKPYYDASQKPKVYLYGPTGFIRAKYKGNDLYEFDDGLFRWVIPIIVGKNKLGQTVFGTISGPILDLYQYKKNISIKVVTNPEDLSLNVKVSTDYQLISGTIDGQNMILFKTSKGYVLYTKEKRNELKNVLSLKIKLPSGKVKTISYDLFTLNGFTTYLRGDFTPYVSSIISTHIVQKGETLWEIAKKYGVRTADLQIINNLENPDKIYSGMKLKIGTVQFTEGLTSIVVNLSTSRLAVYYAGKLVKVFPVAIGRSDSMPPGIYWILNKQIDPALYWYGEYIPPRSPINGLGTRFLQLSNPTYGIHGTTKPWEIGRRISHGCVRMFNFDVEALDAFVDLGSPVLVIKSFEDFPEDISQIPEFLSFKEKIRNKEGESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICX0</accession>
    <name>B7ICX0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polyamine aminopropyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Putrescine aminopropyltransferase</fullName>
            <shortName evidence="3">PAPT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Spermidine synthase</fullName>
            <shortName evidence="3">SPDS</shortName>
            <shortName evidence="3">SPDSY</shortName>
            <ecNumber evidence="3">2.5.1.16</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 8" type="primary">speE</name>
        <name evidence="8" type="ordered locus">THA_1403</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 6">
            <text>putrescine + S-adenosyl 3-(methylsulfanyl)propylamine = H(+) + S-methyl-5'-thioadenosine + spermidine</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer or homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580207.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1403"/>
    <dbReference type="EnsemblBacteria" id="ACJ75847">
        <property type="protein sequence ID" value="ACJ75847"/>
        <property type="gene ID" value="THA_1403"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1403"/>
    <dbReference type="eggNOG" id="COG0421">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048199_0_0_0"/>
    <dbReference type="OMA" id="LWPGQSF"/>
    <dbReference type="OrthoDB" id="1613081at2"/>
    <dbReference type="UniPathway" id="UPA00248">
        <property type="reaction ID" value="UER00314"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="PTHR11558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="1 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="7">
        <location>
            <begin position="19"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-methyl-5'-thioadenosine binding" evidence="3">
        <location>
            <begin position="155"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Spermidine binding" evidence="3">
        <location>
            <begin position="173"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3 4">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="299" mass="34571" checksum="017A90FFCD7B9A42" modified="2009-02-10" version="1">MSEKKIIDGMQYGFVPNRHLWYFEYYTPGNCGIFMKMNKHLYSAQSKYQRIDIFETDFFGRVFSLDGITMTTEKDDFFYHEMLVHVPMFMHPNPKKVLVIGGGDGGSVREVLKHKSVEKVIMCEIDEMVVEAAKKYLPVTASKLDDPRVELVFEDGSQFVRNFKNEFDVIIIDSTDPTAGEGGHLFTEDFYKACFDALKENGTFSAESEDWLYDGAWLRLAYSRIKKVFPVTKLYLGFMPTYPSGMWGYTFASKGVDPIKDFDPEKVKNFGEELKYYNEEIHKAAFALPTFLKKELENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICY3</accession>
    <name>B7ICY3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutaconyl-CoA decarboxylase subunit gamma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1416</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580212.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1416"/>
    <dbReference type="EnsemblBacteria" id="ACJ75860">
        <property type="protein sequence ID" value="ACJ75860"/>
        <property type="gene ID" value="THA_1416"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1416"/>
    <dbReference type="eggNOG" id="COG4770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_5_4_0"/>
    <dbReference type="OMA" id="AIHVGPG"/>
    <dbReference type="OrthoDB" id="1938042at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0092">Biotin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="3">
        <location>
            <begin position="57"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="135" mass="14457" checksum="51C8F73B67F78367" modified="2009-02-10" version="1">MDRKFKVRVNGKEYTVEVEELGGAQSIQSVPTPEPVVENETKSIQTQSTVEEKTAPKQEAASPSSGAKIVAPMSGIVLKVLVSAGQKINSGDKVAILEAMKMENEIISEFSGTVKNVLVKEGDNVDTGQVLIELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICZ1</accession>
    <name>B7ICZ1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1424</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101839.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1424"/>
    <dbReference type="EnsemblBacteria" id="ACJ75868">
        <property type="protein sequence ID" value="ACJ75868"/>
        <property type="gene ID" value="THA_1424"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1424"/>
    <dbReference type="eggNOG" id="COG1896">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_672030_0_0_0"/>
    <dbReference type="OMA" id="ASKWEFD"/>
    <dbReference type="OrthoDB" id="1251692at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="349" mass="40354" checksum="6E161609F3E96319" modified="2009-02-10" version="1">MGYGKLIFMLGNLFTIQRWNNKPAIVKFSEADNAYTTLLISFALRDYYKLDIEPSLKWRLNRILPKLVLSDVSLELKERIERLSPDVWEKVREKAFLELYNIVDQSSITNITSEEFDPIDKFSDISTSLFEAKVNGKVFDFSKPIKELEEKLHPYLKNFSEVSDIPKLIFSIALNMISMTRWNRMYRNIKSTVAGHSFVVTTIAYIISVQLGLSKDKKEEVIKKALLHDLPEAFTGDVITPTKKKTPELDSLVSLVEKQMFNEWVKENKSLSHLEKYEDFAAEPFSGDTGKIVRTADLFAALLECALEIFSGNKEKLFQSVFFDLKKTLKKFKEIDLGQWIDEIEEIVF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID87</accession>
    <name>SYK_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="1">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lysS</name>
        <name type="ordered locus">THA_1523</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 3 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102053.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ID87"/>
    <dbReference type="SMR" id="B7ID87"/>
    <dbReference type="STRING" id="484019.THA_1523"/>
    <dbReference type="EnsemblBacteria" id="ACJ75964">
        <property type="protein sequence ID" value="ACJ75964"/>
        <property type="gene ID" value="THA_1523"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1523"/>
    <dbReference type="eggNOG" id="COG1190">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008255_6_0_0"/>
    <dbReference type="OMA" id="EIFGEKC"/>
    <dbReference type="OrthoDB" id="63621at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034762">
        <property type="entry name" value="Lys-tRNA-ligase_II_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039101">
        <property type="entry name" value="LysRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000199250" description="Lysine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00252"/>
        </source>
    </evidence>
    <sequence length="502" mass="58754" checksum="D31ADE563B1BCE90" modified="2009-02-10" version="1">MLKEFREQRINEIKQISAKGVNPYPYKFEKTHSSQDIKAQFENLNPGEVKEDASVSTAGRIMSLRHHGKSAFFHIKDFFGRIQAYIRQDIVGKDVYEFFKEHIAIGDIVGVKGSVFKSKTGEVTILVKEIELLNKPLRPMPEKWHGIKDKEVLYRQRYVDMIANDETLNRFRIRFEIIKLIREFLNSKGFIEVETPILEYVTGGASARPFITHLNVFDIDMYMRIATELYLKRFIVGGFEKVYELGKNFRNEGLSYKHHPEFTSIEIYQAYADYEDMMNLTEELFVFIVEKLFGTTKVKYQDIEIDFSRPWRRVKMRDFIKEHLGVDILEDTEEKMLEVLKQHDVEVEIKDKGHLIEKLWDLVEDKVVQPTFLLEHPVEISPLAKKHREDPRVTERFELIIYGREMANAFSELNDPVDQYERFLRQAKLREAGDEEAQMMDKDFVRALEYGMPPTGGLGIGIDRLVMLLTNSPTIRDVIAFPLVRPISFEEEEMNLEGGSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID96</accession>
    <name>B7ID96_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide transport system permease protein AppB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1532</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102062.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1532"/>
    <dbReference type="EnsemblBacteria" id="ACJ75973">
        <property type="protein sequence ID" value="ACJ75973"/>
        <property type="gene ID" value="THA_1532"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1532"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_2_0"/>
    <dbReference type="OMA" id="FLMVNVM"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="96"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="321" mass="36236" checksum="EF343BB5DA8C97FF" modified="2009-02-10" version="1">MLRYIARRLIILIPELFIITLIVFLIMQAAPGDFLDQYRLDPSVSEEFLKALEKQYGLDQPVMVQYFKWIKGVLTGDLGYSFYYRRPVVDLIGERVLATLILSLYSFVISWIVGVILGVISALKKYSFWDKFLTVIAFTGIAIPGFFLALLLLYFAAKTGTFPVAGMYDVNHSKMTVWQGFKDIFWHMQLPAFTLTFGGFAGLMRYMRGSLLDVLNEDYVEFARAKGMPERVVIFKHAMRNAINPLITMFGFSLSSLLGGAVITETIFSWPGLGRLVYQALVQQDIYVVMASTIISVIMLIAGNLVGDILLAAVDPRIRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDA6</accession>
    <name>B7IDA6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1543</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1543"/>
    <dbReference type="EnsemblBacteria" id="ACJ75983">
        <property type="protein sequence ID" value="ACJ75983"/>
        <property type="gene ID" value="THA_1543"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1543"/>
    <dbReference type="HOGENOM" id="CLU_3259071_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="42" mass="5051" checksum="AF0ACA35B8D67F17" modified="2009-02-10" version="1">MFYLTIVFSFILSIPIQEIQINIYNYIISWLYSTITLLLHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDC6</accession>
    <name>B7IDC6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Translation initiation factor, eIF-2B alpha subunit-related</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102287.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1565"/>
    <dbReference type="EnsemblBacteria" id="ACJ76003">
        <property type="protein sequence ID" value="ACJ76003"/>
        <property type="gene ID" value="THA_1565"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1565"/>
    <dbReference type="eggNOG" id="COG1184">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092757_0_0_0"/>
    <dbReference type="OMA" id="VADPFKF"/>
    <dbReference type="OrthoDB" id="1723038at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="219" mass="25478" checksum="67F3578E948A5F87" modified="2009-02-10" version="1">MIENFKKLTIQGSHEIAEWILENIKNIEDKESILKEMIKLKPEMQVIKWIYKMIKENKDINNIKEILDTTFENTIKNAKKFLNFDAKVMTISNSRTLEYFFKTHNKKLEIYIPESNPGGEGKILYKNLLNYKKNVYLINDFEVHRYIENCDYVIIGADSVGENGVINKVGSKLLAILSKNFNIPFFVIADQTKFTEVVTEKDRMFEIVPNNLITAIISG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDI7</accession>
    <name>MURA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylglucosamine 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Enoylpyruvate transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylglucosamine enolpyruvyl transferase</fullName>
            <shortName evidence="1">EPT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murA</name>
        <name type="ordered locus">THA_1626</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:18681"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="EC" id="2.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EPSP synthase family. MurA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580305.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDI7"/>
    <dbReference type="SMR" id="B7IDI7"/>
    <dbReference type="STRING" id="484019.THA_1626"/>
    <dbReference type="EnsemblBacteria" id="ACJ76064">
        <property type="protein sequence ID" value="ACJ76064"/>
        <property type="gene ID" value="THA_1626"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1626"/>
    <dbReference type="eggNOG" id="COG0766">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027387_0_0_0"/>
    <dbReference type="OMA" id="CDPHRAT"/>
    <dbReference type="OrthoDB" id="537477at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008760">
        <property type="term" value="F:UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019277">
        <property type="term" value="P:UDP-N-acetylgalactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01555">
        <property type="entry name" value="UdpNAET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00111">
        <property type="entry name" value="MurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005750">
        <property type="entry name" value="UDP_GlcNAc_COvinyl_MurA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01072">
        <property type="entry name" value="murA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000117513" description="UDP-N-acetylglucosamine 1-carboxyvinyltransferase">
        <location>
            <begin position="1"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="21"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="2-(S-cysteinyl)pyruvic acid O-phosphothioketal" evidence="1">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00111"/>
        </source>
    </evidence>
    <sequence length="415" mass="45189" checksum="BC8E361D327A7D2F" modified="2009-02-10" version="1">MGYFKVKNSILNGNVRISGAKNSALPILAATILSSEKLEIENVPDLLDVQTMIEILRKIGKKVIFENNKVTLNGTVENHVVPYDLVRKMRASFNVAGPLAVILGESKVSLPGGCAIGVRPVDYHIMGLKKLGFEVEIEHGEVYIKKGKKEKEVIINLPFPSVGATEHLMTTAALMEGTTVIENAAMEPEIVDLQNFLNSLGAKIQGAGSRKIVIEGVNSLNGGSYKVIPDRIEAGTYAIAIAATGGKGYIEEIIPEHLEILWEILKETGTNVIIEKNRVFVDGEGKKRGININIQPYPGFPTDLQPQILVYLSLADGVSMVVENVFKNRFHHIDELVRMGADIRVVDGTAIVNGVKKLSGTKVEGTDLRATAALIIAGLVAEGTTEVHNDFHVLRGYENIVEKIRHLNGEIEHIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDJ1</accession>
    <name>B7IDJ1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Translation elongation factor G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">fusA2</name>
        <name evidence="5" type="ordered locus">THA_1630</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580309.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1630"/>
    <dbReference type="EnsemblBacteria" id="ACJ76068">
        <property type="protein sequence ID" value="ACJ76068"/>
        <property type="gene ID" value="THA_1630"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1630"/>
    <dbReference type="eggNOG" id="COG0480">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_4_1_0"/>
    <dbReference type="OMA" id="KPKIAYR"/>
    <dbReference type="OrthoDB" id="88967at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="4">
        <location>
            <begin position="5"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="685" mass="75576" checksum="6A24CC7CD28DF191" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDL7</accession>
    <name>B7IDL7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTP pyrophosphokinase (ATP:GTP 3'-pyrophosphotransferase)(PpGpp synthetase I) ((P)ppGpp synthetase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1656</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the relA/spoT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580330.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1656"/>
    <dbReference type="EnsemblBacteria" id="ACJ76094">
        <property type="protein sequence ID" value="ACJ76094"/>
        <property type="gene ID" value="THA_1656"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1656"/>
    <dbReference type="eggNOG" id="COG0317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012300_3_0_0"/>
    <dbReference type="OMA" id="DWISSPK"/>
    <dbReference type="OrthoDB" id="204079at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015969">
        <property type="term" value="P:guanosine tetraphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05399">
        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01668">
        <property type="entry name" value="TGS_RSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004811">
        <property type="entry name" value="RelA/Spo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033655">
        <property type="entry name" value="TGS_RelA/SpoT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13291">
        <property type="entry name" value="ACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00691">
        <property type="entry name" value="spoT_relA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HD" evidence="3">
        <location>
            <begin position="49"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="4">
        <location>
            <begin position="388"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="2">
        <location>
            <begin position="635"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="708" mass="82067" checksum="867709C1553BBEEC" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDM6</accession>
    <name>B7IDM6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Threonine synthase</fullName>
            <ecNumber evidence="5">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">thrC</name>
        <name evidence="10" type="ordered locus">THA_1665</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H2O + O-phospho-L-homoserine = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580335.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1665"/>
    <dbReference type="EnsemblBacteria" id="ACJ76103">
        <property type="protein sequence ID" value="ACJ76103"/>
        <property type="gene ID" value="THA_1665"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1665"/>
    <dbReference type="eggNOG" id="COG0498">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_4_1_0"/>
    <dbReference type="OMA" id="DDVNRFC"/>
    <dbReference type="OrthoDB" id="428821at2"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026260">
        <property type="entry name" value="Thr_Synthase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038945">
        <property type="entry name" value="Thr_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 5 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="cross-link" description="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)" evidence="8">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ76103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="403" mass="44499" checksum="32841F8C95259B80" modified="2009-02-10" version="1">MYKLKCITCGSIYDPEPNMYTCPKCGERYGTLEVIFNYDLINVKKSDFDKHGDISQFSKILPIAEYKVKQVVGNTPTYEFKNYYGIKKVFFKYDGTNPTGSYKDRASAIAISKAYEYGYDTIYCASTGNAASSLAGLSAPTDLKTYIFVPKNIPIAKLAQLHVFGANIILIDGNYDQAFDISTKVGKKLNWYSRNSAINPYLLEGKKTGAMELVVQLDFNVPDIVFVSAGDGTVISSIYKGFYDFYNLNLIQKIPKIIGIQAEGADAIAKTFSYGKPYTPVDIIPNTIADSISVGKPRDVIKACKYVEKSGGTFLTVNDEEIKTAIFELAKNTGIFAEPAGAAAYAGFKKAISMNLLKKEDTVAIFITGNGLKDIKAVQDNIKINPIPPVFEKIIEYINEVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDQ9</accession>
    <name>B7IDQ9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminotransferase, class II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">patB</name>
        <name evidence="2" type="ordered locus">THA_1699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102549.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1699"/>
    <dbReference type="EnsemblBacteria" id="ACJ76136">
        <property type="protein sequence ID" value="ACJ76136"/>
        <property type="gene ID" value="THA_1699"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1699"/>
    <dbReference type="eggNOG" id="COG1168">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_15_0_0"/>
    <dbReference type="OMA" id="RINIGCP"/>
    <dbReference type="OrthoDB" id="1462898at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027619">
        <property type="entry name" value="C_S_lyase_PatB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04350">
        <property type="entry name" value="C_S_lyase_PatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="36"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="384" mass="44380" checksum="0863E3C590BBC882" modified="2009-02-10" version="1">MIDFDKTLERRGTNSYKWDYGYNELYDNLLPLWVADMDFEAPKEVIDAIKARAKHGIYGYTFRPEGYYKSIIKWFDEIHNVKIKKDWIVPIPGVVPGIAFAIQALTNPGDKIIIQPPVYRPFYEVINNLGRRISLNPLIENNGYYKMDFENLEKIIDNRTTMLILCSPHNPVGRVWKKTELEKLGEICLKHNITVVSDEIHADIVYSPNKHNMFFSVSDKFLNNSIVLTAPNKTFNIAGLQTGNAFIPNKFIRQKFEAVINSQHLNLTNIFGIVATEAAYTYGKSWLNQLKKYLYDNAVYVKKALEKNTNVRTLIPEGTFLMWLDLRKYNTDIHEKLLKNGLWLNQGKEFGKEGDGFERMNIATSRKIIEKAVKIIISSLNDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDR3</accession>
    <name>B7IDR3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1703</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580361.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1703"/>
    <dbReference type="EnsemblBacteria" id="ACJ76140">
        <property type="protein sequence ID" value="ACJ76140"/>
        <property type="gene ID" value="THA_1703"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1703"/>
    <dbReference type="eggNOG" id="COG3437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_494251_0_0_0"/>
    <dbReference type="OMA" id="MITIIES"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029151">
        <property type="entry name" value="Sensor-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103190">
        <property type="entry name" value="SSF103190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="HD-GYP" evidence="3">
        <location>
            <begin position="352"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="321"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="551" mass="64119" checksum="A1EF8E45C09E9181" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDS3</accession>
    <name>MIAA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dimethylallyl diphosphate:tRNA dimethylallyltransferase</fullName>
            <shortName evidence="1">DMAPP:tRNA dimethylallyltransferase</shortName>
            <shortName evidence="1">DMATase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl-diphosphate:tRNA isopentenyltransferase</fullName>
            <shortName evidence="1">IPP transferase</shortName>
            <shortName evidence="1">IPPT</shortName>
            <shortName evidence="1">IPTase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">miaA</name>
        <name type="ordered locus">THA_1714</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = diphosphate + N(6)-dimethylallyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102573.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDS3"/>
    <dbReference type="SMR" id="B7IDS3"/>
    <dbReference type="STRING" id="484019.THA_1714"/>
    <dbReference type="EnsemblBacteria" id="ACJ76150">
        <property type="protein sequence ID" value="ACJ76150"/>
        <property type="gene ID" value="THA_1714"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1714"/>
    <dbReference type="eggNOG" id="COG0324">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032616_0_1_0"/>
    <dbReference type="OMA" id="YAKRQYT"/>
    <dbReference type="OrthoDB" id="1069591at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="PTHR11088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000191871" description="tRNA dimethylallyltransferase">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <begin position="33"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00185"/>
        </source>
    </evidence>
    <sequence length="301" mass="35348" checksum="1F5C4EDBE86984B1" modified="2009-02-10" version="1">MKYTIISGPTAVGKTDIVLEIASQINANIISVDSRQIYKLMDIGTAKPSKEEKSKVTHYLIDHIYPDEYYNAFLFRQDALKIRDKLVNEGIVPLFVGGTGLYIDSLVRGFFEGVPKDEKLRKELSEMEKNEPGILRSMLEKYDPQAAQKIHPSDIKRTIRALEVYFKTGKKISQLQTQSEYSKDYKILVLDRYRDELYERINLRVDKMIKEGLVDEVKSLLEKYPKDLNAFQTIGYKEIIRYLENTYDLNTAVHLIKKNTRHYARRQIIWLRRYKQAKWINLSEISRKKAIEEIKKFILEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDS5</accession>
    <name>B7IDS5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1716</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102578.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1716"/>
    <dbReference type="EnsemblBacteria" id="ACJ76152">
        <property type="protein sequence ID" value="ACJ76152"/>
        <property type="gene ID" value="THA_1716"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1716"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1110275_0_0_0"/>
    <dbReference type="OMA" id="CTGFSCI"/>
    <dbReference type="OrthoDB" id="1433122at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="246" mass="28341" checksum="2BD864CA5BDD57A1" modified="2009-02-10" version="1">MTLAFGGDSILYLSIPAFFSAAEKYNIEFEEIHCTGFSCIPAYFYLKTQSSSTSYLYSKKLYLELKKTFHFLFGLNLFGITEQLRTLYKASRSLNGFKSEKVLINFVDKFFVDEPIPEKLKIHAFNINTFNDEILKGSLKEALIKTLSIPIEFKPYNGYITGSWVYGIPEADFLFYIENENEYTLKNAVDYMIVSTIHRTKQIAKKRLENANHKIVINTKSKNPFEISTTILNISEKYLGGLINEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDS7</accession>
    <name>END4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable endonuclease 4</fullName>
            <ecNumber evidence="1">3.1.21.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Endodeoxyribonuclease IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Endonuclease IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nfo</name>
        <name type="ordered locus">THA_1718</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.</text>
            <dbReference type="EC" id="3.1.21.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 3 Zn(2+) ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.2" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102582.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDS7"/>
    <dbReference type="SMR" id="B7IDS7"/>
    <dbReference type="STRING" id="484019.THA_1718"/>
    <dbReference type="EnsemblBacteria" id="ACJ76154">
        <property type="protein sequence ID" value="ACJ76154"/>
        <property type="gene ID" value="THA_1718"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1718"/>
    <dbReference type="eggNOG" id="COG0648">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025885_0_1_0"/>
    <dbReference type="OMA" id="HPGSHLK"/>
    <dbReference type="OrthoDB" id="1088517at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008833">
        <property type="term" value="F:deoxyribonuclease IV (phage-T4-induced) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00152">
        <property type="entry name" value="Nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="PTHR21445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00587">
        <property type="entry name" value="nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00729">
        <property type="entry name" value="AP_NUCLEASE_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00730">
        <property type="entry name" value="AP_NUCLEASE_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00731">
        <property type="entry name" value="AP_NUCLEASE_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000118101" description="Probable endonuclease 4">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00152"/>
        </source>
    </evidence>
    <sequence length="282" mass="31920" checksum="0160009E7D19C112" modified="2009-02-10" version="1">MIRIGAHMPIGGGFKRVPKETYEIGGNAFQIFPHNPRQWKANLPKEEDIKLFKEGIKKYKIDPEAMLCHSGYLINIASPNIETWNKSLELLILEMKICSTLGIKYLNIHPGSHLKSGVENGINQILKALNTAMEKEKNTFILLENVTKKGGNIGSTLEELKLIIDKAKFPDRLGITIDTCHAFDAGYDITNKEKLNEFLNKIDKYFSLEKLKFIHLNDSKNDLGSNKDRHENIGKGKIGSEGLKVFISHPLIQKIPWVLETPGDNDVHKKDIQEVLKILEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDU2</accession>
    <name>B7IDU2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580377.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1734"/>
    <dbReference type="EnsemblBacteria" id="ACJ76169">
        <property type="protein sequence ID" value="ACJ76169"/>
        <property type="gene ID" value="THA_1734"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1734"/>
    <dbReference type="HOGENOM" id="CLU_1077404_0_0_0"/>
    <dbReference type="OMA" id="MHYENAL"/>
    <dbReference type="OrthoDB" id="1820048at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="258" mass="29482" checksum="5264C18A963D63A8" modified="2009-02-10" version="1">MRKSFVVFLIILGLFVFSMRTVKSPVDLLEGQSGVGVKFGVMFLDNWLTNFELDYYDHENYLNGLLYLRTENSESESINSIGYVIKGMSKNFQWGTNFVFNSLTTESTVSRSLSVGIGFEAALSNYFNIGAFIDDLPLYAQKPFTFFKPNVGIDFSFGTSNFNLYGALKYIKQEYIQFNLGPVISFSTLNFGFFWVPEYIIKDNLMLNKISGDFKVELETFSVSIFGFYAFDKNVSVDIPMHYEAAPYGYEVNIEVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDV9</accession>
    <name>B7IDV9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1752</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102654.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1752"/>
    <dbReference type="EnsemblBacteria" id="ACJ76186">
        <property type="protein sequence ID" value="ACJ76186"/>
        <property type="gene ID" value="THA_1752"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1752"/>
    <dbReference type="eggNOG" id="ENOG502ZE6V">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1517216_0_0_0"/>
    <dbReference type="OMA" id="IYRIGCK"/>
    <dbReference type="OrthoDB" id="1716308at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="177" mass="20925" checksum="BB86AF42ABDDB8DD" modified="2009-02-10" version="1">MTIIEFINKNLSGIYLLAKDHNKNDYQVRLINIQKTGVVEIESNEQFNIGKTLQLNIPVKEALLLFVGEVFDIKEKRHFLKTHEKVGIIQRRKEKRYPYFKRATFSNNKILIIDVSKSGMQIFSEKEMELKSSYEIAIDDKKINIIPMWRIYEEEIYRIGCKVSNESLNLWNNILNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDW3</accession>
    <name>B7IDW3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA alkylation repair enzyme superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1759</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102658.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1759"/>
    <dbReference type="EnsemblBacteria" id="ACJ76190">
        <property type="protein sequence ID" value="ACJ76190"/>
        <property type="gene ID" value="THA_1759"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1759"/>
    <dbReference type="eggNOG" id="COG4912">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079880_0_0_0"/>
    <dbReference type="OMA" id="NWDLVDT"/>
    <dbReference type="OrthoDB" id="1933111at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014825">
        <property type="entry name" value="DNA_alkylation"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08713">
        <property type="entry name" value="DNA_alkylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="229" mass="27327" checksum="21784AA48F7C6542" modified="2009-02-10" version="1">MLEKIIKELENNSNEKKAKASYKFLKAFPGGYGEGDEFLGLTVPIQRKIAKKYKNLPLEDVEKLLQSKYHEHRLTALFILILNYKNKKEEVINIYLRNLDRVNNWDLVDSSAPYLLGPYLENKDKSILYELAKSDNLWKQRVAIITTFYFIKKNDFKDALKISELLLNHKHDLIHKAVGWMLREIGKRDKKVEEEFLKKYYKKMPRTMLRYAIEKFPEEERQKILKGIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDY8</accession>
    <name>B7IDY8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hdod domain family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580409.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1784"/>
    <dbReference type="EnsemblBacteria" id="ACJ76215">
        <property type="protein sequence ID" value="ACJ76215"/>
        <property type="gene ID" value="THA_1784"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1784"/>
    <dbReference type="eggNOG" id="COG1639">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048246_4_2_0"/>
    <dbReference type="OMA" id="ANSARFH"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013976">
        <property type="entry name" value="HDOD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08668">
        <property type="entry name" value="HDOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51833">
        <property type="entry name" value="HDOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HDOD" evidence="1">
        <location>
            <begin position="12"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="285" mass="32005" checksum="88933951235F1F8E" modified="2009-02-10" version="1">MIAELLKDIEELPTPDFHVQQIINIASNPDASVKTLEKAISTEPTLSLKVLKLVNSAYYGLPKKVTKISEAVMILGFKTVRNLALSLFTYSSLNRGTSKIDKKMLWKHFMSTAIISEVVAKNIGYPEQEEAFMAGLLHDVGKIVLDIAFPEYLTMIVNKSRESKINLYKLEKMLEVETHNEIGSYLIEKWQLPELYQIVSKFHDEPEENTSPGFNSIVFIVHLSNYISNLLYPGYSGSFGDPILSKDTFKVLGIKPSDLLKLIVKSKDSLAKAKDLMEGGEDNET</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDZ1</accession>
    <name>ENGB_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable GTP-binding protein EngB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">engB</name>
        <name type="ordered locus">THA_1787</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Necessary for normal cell division and for the maintenance of normal septation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580412.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDZ1"/>
    <dbReference type="SMR" id="B7IDZ1"/>
    <dbReference type="STRING" id="484019.THA_1787"/>
    <dbReference type="EnsemblBacteria" id="ACJ76218">
        <property type="protein sequence ID" value="ACJ76218"/>
        <property type="gene ID" value="THA_1787"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1787"/>
    <dbReference type="eggNOG" id="COG0218">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033732_3_0_0"/>
    <dbReference type="OMA" id="LMMDIRH"/>
    <dbReference type="OrthoDB" id="1746051at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00321">
        <property type="entry name" value="GTPase_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03598">
        <property type="entry name" value="GTPase_YsxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0717">Septation</keyword>
    <feature type="chain" id="PRO_1000119598" description="Probable GTP-binding protein EngB">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="EngB-type G" evidence="1">
        <location>
            <begin position="23"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="31"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="57"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="75"/>
            <end position="78"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="143"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="173"/>
            <end position="175"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00321"/>
        </source>
    </evidence>
    <sequence length="194" mass="22664" checksum="10743E7E1556DD54" modified="2009-02-10" version="1">MKIKSVELVKTIYKSNDKYPESLNGEFVFVGRSNVGKSTLLNILTGRNIAKTSKKPGKTASINFYKINNSFYFVDLPGYGYAKVSVEERARWRKIIENYFSKRAWNIKLVFVLIDGRHELQKNDEILLEWLKELELDFAIVMTKMDKLKNSERAKMIRYYKDLYGENYTIIPYSAITREGIDKIYELIEIFGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDZ5</accession>
    <name>B7IDZ5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PSP1 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1791</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580416.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1791"/>
    <dbReference type="EnsemblBacteria" id="ACJ76222">
        <property type="protein sequence ID" value="ACJ76222"/>
        <property type="gene ID" value="THA_1791"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1791"/>
    <dbReference type="eggNOG" id="COG1774">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033149_2_0_0"/>
    <dbReference type="OMA" id="YGGLGPC"/>
    <dbReference type="OrthoDB" id="1523756at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007557">
        <property type="entry name" value="PSP1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04468">
        <property type="entry name" value="PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51411">
        <property type="entry name" value="PSP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP1 C-terminal" evidence="1">
        <location>
            <begin position="64"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="294" mass="33705" checksum="0AECAB0CB731324B" modified="2009-02-10" version="1">MELTAEVYGVELIPMGPIIYYTENGEDIKVNDTVIVISEFGLDYGVVRIGKRQMSIDDIGYDLKPIIRKATEEDLETIEKNKEIAKRAFEVTKQLVKKHGLNMKVLYSKMMVDGSRLVIYFSSKNRVDFRELVKDIAKEFKTRIELRQVGARDEMKFIKGLGLCGKKSCCSYWLRSFDSITLKHAKRQQMMINTAKITGPCGRLLCCLKFEYDFYIDALKNIPDEGSMIKYNGKDAKVITVNVFLKKVTIYTKDGETIALPFDYFRGGNNVEIVDDNRDNIDLDITDDVFNGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDZ9</accession>
    <name>B7IDZ9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Biotin transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1795</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BioY family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580420.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1795"/>
    <dbReference type="EnsemblBacteria" id="ACJ76226">
        <property type="protein sequence ID" value="ACJ76226"/>
        <property type="gene ID" value="THA_1795"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1795"/>
    <dbReference type="eggNOG" id="COG1268">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077931_3_0_0"/>
    <dbReference type="OMA" id="FWPVPMT"/>
    <dbReference type="OrthoDB" id="1691691at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015225">
        <property type="term" value="F:biotin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003784">
        <property type="entry name" value="BioY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34295">
        <property type="entry name" value="PTHR34295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02632">
        <property type="entry name" value="BioY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016661">
        <property type="entry name" value="BioY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR016661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="167" mass="18122" checksum="520BACFB6D9AFD8A" modified="2009-02-10" version="1">MKTKDISLVALFVTLTIVGAQISIPIGTVPITLQVLMVFLTGYFLKPTLALLTQSIYLLLGIIGLPVFANFSGGFASIIGPTGGYLIAFPLAAWIISLSKKDYFSMFLHGILGIAIIYTLGVLVLNYHLHDFKKAFMIGVVPFIGIDFLKMCLAVIISKRVLKVVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE00</accession>
    <name>B7IE00_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Malonyl CoA-acyl carrier protein transacylase</fullName>
            <ecNumber evidence="3">2.3.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fabD</name>
        <name evidence="6" type="ordered locus">THA_1796</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>holo-[ACP] + malonyl-CoA = CoA + malonyl-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fabD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.39" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580421.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1796"/>
    <dbReference type="EnsemblBacteria" id="ACJ76227">
        <property type="protein sequence ID" value="ACJ76227"/>
        <property type="gene ID" value="THA_1796"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1796"/>
    <dbReference type="eggNOG" id="COG0331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030558_0_1_0"/>
    <dbReference type="OMA" id="AANYNCP"/>
    <dbReference type="OrthoDB" id="1123553at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024925">
        <property type="entry name" value="Malonyl_CoA-ACP_transAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004410">
        <property type="entry name" value="Malonyl_CoA-ACP_transAc_FabD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000446">
        <property type="entry name" value="Mct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="SSF55048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00128">
        <property type="entry name" value="fabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_AT" evidence="5">
        <location>
            <begin position="5"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000446-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="297" mass="33547" checksum="B72821DF69194F00" modified="2009-02-10" version="1">MKAFLFPGQGSQYSGMAKDFSKYPFWNDLSKRAEEVLNIDLINIMNGDEETLKLTENAQPAIFLASFVAFKHLESLGINYDIFAGHSLGEYTAFAAAGVYDFDFGIYLVRKRGEYISQAIEPGKGSMAAVLKISAEKVEELVKNYEGLYVANYNSSTQTVISGLKSSIQKFIEDCKNKGIRATELVISGPFHTPFLESAREKLSKEIEHVKFKKPKVPIVLNSTAKESTDPEELKYYLLEQIAGPVYWLQSIRRMLELGVEEFIEVGPKNVLSNMLKREKIDIIHFSEIEEIEDSRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2017-03-15" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE18</accession>
    <name>MURJ_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipid II flippase MurJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">murJ</name>
        <name evidence="6" type="synonym">mviN</name>
        <name evidence="6" type="ordered locus">THA_1814</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2017" name="Nat. Struct. Mol. Biol." volume="24" first="171" last="176">
            <title>Crystal structure of the MOP flippase MurJ in an inward-facing conformation.</title>
            <authorList>
                <person name="Kuk A.C."/>
                <person name="Mashalidis E.H."/>
                <person name="Lee S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="28024149"/>
            <dbReference type="DOI" id="10.1038/nsmb.3346"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)</scope>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>TOPOLOGY</scope>
        <scope>MUTAGENESIS OF SER-17; ARG-18; ARG-24; ARG-52; GLU-57; ASN-162; GLN-229; ASN-231; ARG-255; PHE-256 AND LEU-259</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.</text>
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        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
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<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
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            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
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                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
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        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="Membr_insert_YidC/Oxa1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="PTHR12428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="373"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="434" mass="50542" checksum="61E8B86596B4F33C" modified="2009-02-10" version="1">MKRIIAVIFLFATLIMIAGTISVEERTDGIYVFTKFMEYKFDYNGNLSEVYRTIERRTRMFLYSNDGFDIEGVSSTPTFSWQGLKDGDKYTEVTLKFKYSDEIEKIYNFKEGPNYTFDVSVLSSKEVVVHVPRVGFENDDRLRNNIFLSFYSKGDIISIIKFNGNTLGGNSVKGSNLRFLVYIGPYKKTLIKSAFSDDYDLISEMLKTVSGVGGWFDFILYPLVYFFGWINDFTRNFGLTIIVFTIIVRFVLYPLYHSQTKSMIKMRKLQPIVEQIKKKYKDPQKQQQELLKAYRENKINPASGCLMAFVQLPIFIILYQVIRYYQEEFAFSGRFLFWNDLTAGGFSANWIFLVIQIVAGYYLALITSQDSRTAWQSIIMSFIFPFLFIGLPSGVFLYYTANTLIQLGITYYIYKRYKIKGITQRELWGLPNKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE60</accession>
    <name>B7IE60_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ggdef domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1861</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580462.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1861"/>
    <dbReference type="EnsemblBacteria" id="ACJ76287">
        <property type="protein sequence ID" value="ACJ76287"/>
        <property type="gene ID" value="THA_1861"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1861"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006091_0_0_0"/>
    <dbReference type="OMA" id="YTYFLEG"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="1207"/>
            <end position="1337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="1338" mass="158521" checksum="A2DA2E4C38EED289" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE80</accession>
    <name>B7IE80_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pega domain family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1881</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102880.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1881"/>
    <dbReference type="EnsemblBacteria" id="ACJ76307">
        <property type="protein sequence ID" value="ACJ76307"/>
        <property type="gene ID" value="THA_1881"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1881"/>
    <dbReference type="eggNOG" id="ENOG5032X3A">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_959310_0_0_0"/>
    <dbReference type="OMA" id="WSININL"/>
    <dbReference type="OrthoDB" id="974335at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013229">
        <property type="entry name" value="PEGA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08308">
        <property type="entry name" value="PEGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="PEGA" evidence="2">
        <location>
            <begin position="113"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="307" mass="35156" checksum="0CE2A4B8154B73AD" modified="2009-02-10" version="1">MTFKRLGFLLLLIFSVSSFSTVNWSININLNLGTPQNIPDYSILIDTGVSFVDVYIDDVYAGQTDIFGQLLVNFDTSGYHTITIKDEDYLNISVLVFVDKTGKYLKIPLVEAGKLVVFSNTYPVNVYINGYYYGTIRSIDEEIKLPEGEHFVTFESPGYNPITKKVYIKFEEKGAVNLNFEKLKLELNIRSKINEFSPNNDWYMDRWELEIYLSTYATVTVDILKDDIKIFEKVFSGKPNLNLFTWDGSGASEVGEYLVKVIANDGNDEIVKETKVLLDDKYTYLKEIAITSLIMMFGTILYFVLKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE81</accession>
    <name>RF2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide chain release factor 2</fullName>
            <shortName evidence="1">RF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">prfB</name>
        <name type="ordered locus">THA_1882</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Methylated by PrmC. Methylation increases the termination efficiency of RF2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102881.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IE81"/>
    <dbReference type="SMR" id="B7IE81"/>
    <dbReference type="STRING" id="484019.THA_1882"/>
    <dbReference type="PRIDE" id="B7IE81"/>
    <dbReference type="EnsemblBacteria" id="ACJ76308">
        <property type="protein sequence ID" value="ACJ76308"/>
        <property type="gene ID" value="THA_1882"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1882"/>
    <dbReference type="eggNOG" id="COG1186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_6_0_0"/>
    <dbReference type="OMA" id="YVFHPYQ"/>
    <dbReference type="OrthoDB" id="928964at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00094">
        <property type="entry name" value="Rel_fac_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004374">
        <property type="entry name" value="PrfB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00020">
        <property type="entry name" value="prfB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000117264" description="Peptide chain release factor 2">
        <location>
            <begin position="1"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="1">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00094"/>
        </source>
    </evidence>
    <sequence length="369" mass="42845" checksum="D1BC7C0573CB195B" modified="2009-02-10" version="1">MIDFELKQKIDEAKKKFEDIIKVFHPENKRKELEELEKQMGDSDFWSDQRKAREISQKAQRIRKIIDDMKDIEAKFEDLDAAIELSDEDQSFVETIKEMVEEIEKKVKTFELELILNGKFDASNAYLTIHPGAGGTESQDWASMLLRMYMRWAERKGFDVQIVDYQPGEEAGIKSAMIYIKGDYAYGYLKYERGVHRLVRISPFDANKRRHTSFASVNVIPEIDDDIDIEINPEDLRIDTYRASGAGGQYVNKTESAVRITHIPTGIVVTCQTERSQLQNKETALKVLKARLYQLELEKRQKQLEEIQGELKDISWGNQIRSYVFQPYTMVKDHRTNVETGNIDAVMDGEIDIFIESELIHFAGIRNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEA0</accession>
    <name>B7IEA0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Valine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Valyl-tRNA synthetase</fullName>
            <shortName evidence="8">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 13" type="primary">valS</name>
        <name evidence="13" type="ordered locus">THA_1901</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The C-terminal coiled-coil domain is crucial for aminoacylation activity.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102918.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1901"/>
    <dbReference type="EnsemblBacteria" id="ACJ76327">
        <property type="protein sequence ID" value="ACJ76327"/>
        <property type="gene ID" value="THA_1901"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1901"/>
    <dbReference type="eggNOG" id="COG0525">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_0_2_0"/>
    <dbReference type="OMA" id="FATKLWN"/>
    <dbReference type="OrthoDB" id="32262at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02004">
        <property type="entry name" value="Val_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019499">
        <property type="entry name" value="Val-tRNA_synth_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="PTHR11946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10458">
        <property type="entry name" value="Val_tRNA-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1" evidence="10">
        <location>
            <begin position="17"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="11">
        <location>
            <begin position="603"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="domain" description="Val_tRNA-synt_C" evidence="12">
        <location>
            <begin position="799"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="797"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="523"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="526"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ76327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="864" mass="100674" checksum="2EBBB76C92CA6B86" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEB7</accession>
    <name>B7IEB7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-aspartate dehydrogenase</fullName>
            <ecNumber evidence="6">1.4.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nadX</name>
        <name evidence="9" type="ordered locus">THA_1920</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + L-aspartate + NAD(+) = H(+) + NADH + NH4(+) + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:11788"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + L-aspartate + NADP(+) = H(+) + NADPH + NH4(+) + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:11784"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the L-aspartate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.21" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580482.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1920"/>
    <dbReference type="EnsemblBacteria" id="ACJ76344">
        <property type="protein sequence ID" value="ACJ76344"/>
        <property type="gene ID" value="THA_1920"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1920"/>
    <dbReference type="eggNOG" id="COG1712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089550_0_0_0"/>
    <dbReference type="OMA" id="ECAGHSA"/>
    <dbReference type="OrthoDB" id="1670363at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00456"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033735">
        <property type="term" value="F:aspartate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01265">
        <property type="entry name" value="NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002811">
        <property type="entry name" value="Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022487">
        <property type="entry name" value="Asp_DH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020626">
        <property type="entry name" value="Asp_DH_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011182">
        <property type="entry name" value="L-Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01958">
        <property type="entry name" value="DUF108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005227">
        <property type="entry name" value="Asp_dh_NAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03855">
        <property type="entry name" value="NAD_NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6" id="KW-0521">NADP</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_3" evidence="8">
        <location>
            <begin position="50"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="DUF108" evidence="7">
        <location>
            <begin position="144"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="244" mass="27562" checksum="163FDBF82F931E93" modified="2009-02-10" version="1">MKPLFLGFGNAANVILKELEGYIQDFYYFDEKNVTSSKGKKLEKFFIPDDCSFVVECASVEAVKEYYRDIIISNKDFYILSTGAFADENFRNDFFRFIENSTSKIYIPSGAIGGIDVINAIKKYIEKVVLRTRKPPKAFSRDDKEKMLIFSGDSIEAIKRFPKNTNVSVTLALSVGDFRKVHVEIYSDPKVEKNIHEVEVVSSVGNYKFIFSNNPSNNPKTSLLAPLSLAALIKKNFENVKIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEB9</accession>
    <name>B7IEB9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nicotinate-nucleotide diphosphorylase (carboxylating)</fullName>
            <ecNumber evidence="4">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nadC</name>
        <name evidence="12" type="ordered locus">THA_1922</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102951.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1922"/>
    <dbReference type="EnsemblBacteria" id="ACJ76346">
        <property type="protein sequence ID" value="ACJ76346"/>
        <property type="gene ID" value="THA_1922"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1922"/>
    <dbReference type="eggNOG" id="COG0157">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039622_2_0_0"/>
    <dbReference type="OMA" id="DMIMLKD"/>
    <dbReference type="OrthoDB" id="1718114at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="PTHR32179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="QRPTase_N" evidence="11">
        <location>
            <begin position="23"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="QRPTase_C" evidence="10">
        <location>
            <begin position="100"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ76346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="271" mass="30752" checksum="2C076BB66E25DFA1" modified="2009-02-10" version="1">MVEKIIEWIRKDEGLIDFSAYPLKGKVAKSIIILKDSAVLSGLELVKEVFKRFNIEFETKYEDGMYVNKNSIIFTLMGDAYYLLVIERSVLNLLSLMSGVATKVAKLSKKAKEYNIVIAATRKTIPFVGDLQKIAVLHGGGNTHRLNLSDTVMIKDNHKKIYKSIRKAVEAVKVIKPFTSKIEVEVEDFEELRECLDLEVDIIMLDNFSPEDIKEAIKIIKEKRKDIIIEVSGGINEKNLDEYLIKGVDVISIGKLTSEVQYVDFSMEVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEC1</accession>
    <name>B7IEC1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ggdef family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580485.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1924"/>
    <dbReference type="EnsemblBacteria" id="ACJ76348">
        <property type="protein sequence ID" value="ACJ76348"/>
        <property type="gene ID" value="THA_1924"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1924"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1642911_0_0_0"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="125"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="161" mass="19155" checksum="67A644AF2007A72A" modified="2009-02-10" version="1">MFFVAILISYFLSKKLSKNIGYYLKKISTTLSKFSEKHFETVEVEEGMEEEIKVFVNTFNSVSNKLKYTLENIENIIFQKTNELKEKNKMLSELSIRDLLTNLYNRREFNQKFPKDFSLSKRENMYLNFAIIDIYHFKKINDCHGHLAGDTCLKNFQKFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEC6</accession>
    <name>B7IEC6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Bacterial extracellular solute-binding protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1930</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580488.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1930"/>
    <dbReference type="EnsemblBacteria" id="ACJ76353">
        <property type="protein sequence ID" value="ACJ76353"/>
        <property type="gene ID" value="THA_1930"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1930"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_556373_0_0_0"/>
    <dbReference type="OMA" id="TTFWSFD"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="470" mass="54681" checksum="ACD96BC032355FEC" modified="2009-02-10" version="1">MNKISVLFGFLMIFFAILAFSETVVTVGVFSWDVPIFQQIAEEFEKENPDIKIEIVEIPWTPGDIINSLLTAKAASGEKFPDVIAQSWEPIVYPVSQGWVYPLDEFIKNDPDYNYVPESIQNAFKFLGKTYALGERLHFNTIVLNLDLLEKLNLPVPSYDWDINTFMYLARRATNREYSGIDNLWEFDAYMAAVFSDRTTFWSFDFNKKRFDLINGGWLKAVEIQEKLRKIPGLDASMLFNQQLRDQGELDDYQKKFGEDTDAFFEGKILMGFKGTWDWDEEGLKSLPWKMDMYPLPHDHEIGMRQPIHINYAFMTSTTKHPKEAFKFLKYITYDPKGVVVKFKYVTSVTDAIGNSFWFVPATMHPEVVKEFKEAKFIPNGVKYMLENLDKTVQVDMWKVIPGWFQAINDVMIPTEEKIRSGDAEPTAIAAETEEKLNEIIEQAWNEFIKQVEEVQKNFSKLRKKIESEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEE1</accession>
    <name>B7IEE1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Catabolite gene activator and regulatory subunit of cAMP-dependent protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1946</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580494.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1946"/>
    <dbReference type="EnsemblBacteria" id="ACJ76368">
        <property type="protein sequence ID" value="ACJ76368"/>
        <property type="gene ID" value="THA_1946"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1946"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075053_3_2_0"/>
    <dbReference type="OMA" id="VLCRDFG"/>
    <dbReference type="OrthoDB" id="1596937at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13545">
        <property type="entry name" value="HTH_Crp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51063">
        <property type="entry name" value="HTH_CRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="9"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="HTH crp-type" evidence="3">
        <location>
            <begin position="145"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="227" mass="25990" checksum="AD53A4BB82A363F0" modified="2009-02-10" version="1">MEIFSYESIFDKEKQNELRNVFLALAKKGKQIEVGKNCEIELSNESSIAIVVEGKLKQSLTNKEGLEKILFYLQPGEIFGEVDYFGETKFNTITIAVVNSKISILEKKIIEEQLTASPILYRYFMKSIIRKLRIIEFQMLNMTFNDSIGRISNTLLRLAAQQGIKKDGKVIINFPLTQQELANIIGCSRITVTRTFKDLISKGIISIKNKKIIIKNLKELEKLDKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEF4</accession>
    <name>B7IEF4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1959</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580507.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1959"/>
    <dbReference type="EnsemblBacteria" id="ACJ76381">
        <property type="protein sequence ID" value="ACJ76381"/>
        <property type="gene ID" value="THA_1959"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1959"/>
    <dbReference type="eggNOG" id="COG3649">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078173_0_0_0"/>
    <dbReference type="OMA" id="SNYNADF"/>
    <dbReference type="OrthoDB" id="598606at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006482">
        <property type="entry name" value="Cas7_Csh2/Csh2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05107">
        <property type="entry name" value="Cas_Cas7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="301" mass="34823" checksum="506636B710112E81" modified="2009-02-10" version="1">MFLKRCYGIFVLKSENSNWNADFTKYPRRLPDENATIFATDKSAKYAIRKYFVDLGERVFVWRTFKEDGQPKSLDETYKYWIEVLKDEVKMIKNNKGEEKEAIDKKDFFNKFIDVKLFGITFAVGSQQMSITGPLQISYGVNRYNVNVPYVVDIASPFRNKETDMQTTLGNEIKNLKSYYVYDFVLNPKNLPEGMEISDDEIKKLKDALKKFATNLNTTSKIGTENALLLFVTLKEGSNLVLPTMKNLVNVSEDEILDLSKISELLNKYKDEIEEVEVYYNDNICKVNGIDGSWRKGDILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEF8</accession>
    <name>B7IEF8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sugar ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1964</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580511.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1964"/>
    <dbReference type="EnsemblBacteria" id="ACJ76385">
        <property type="protein sequence ID" value="ACJ76385"/>
        <property type="gene ID" value="THA_1964"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1964"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_6_0_0"/>
    <dbReference type="OMA" id="IYSNPML"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="417" mass="48224" checksum="A40B9FB80E48055D" modified="2009-02-10" version="1">MKKLLIFSLVLLSLFAFSAKIVFWTAPNPLQEEFWKPLIEEWNNNHLDAQIDWKTIPAAGSSEEAILTAVAAGSAPDFCTNIFSGFAVQLAEQGIIMPLDEEYGQEFFDLVKARKMENIVEGWKLNNHYYVFPIYSNPILMWWRKDKLQELGFDKPPRTYSDIYEVAKKYANPPDRYAMQVVQGRNWWDRWFDFITYYYAASSGKSYIDTKLYRATINDEYGKAVAEFIYTMFKNKWPAVEMGNQFSLSTGKALGVLQGPWSLNWAKNTYPEVYKNIWISPPPVPDNYSGGPIKTFADTKGLVVFSHTKYKKEIFEFIKWVFSNTENDVRWIEITKMPPAREDLATNPLFEKYMKDDPYFAAYAKEVGNAIPPALISNTIDVQDAMTVYLIEPLMYLKSSPEDALNKCVKEINKILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEG5</accession>
    <name>B7IEG5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1971</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580516.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1971"/>
    <dbReference type="EnsemblBacteria" id="ACJ76392">
        <property type="protein sequence ID" value="ACJ76392"/>
        <property type="gene ID" value="THA_1971"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1971"/>
    <dbReference type="eggNOG" id="COG1331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_384914_0_0_0"/>
    <dbReference type="OMA" id="YFPANEI"/>
    <dbReference type="OrthoDB" id="568106at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="654" mass="73858" checksum="D94D1B45B4511800" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEJ3</accession>
    <name>B7IEJ3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type III restriction enzyme R protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1999</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580536.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1999"/>
    <dbReference type="REBASE" id="19389">
        <property type="entry name" value="TafTCORF2000P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76420">
        <property type="protein sequence ID" value="ACJ76420"/>
        <property type="gene ID" value="THA_1999"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1999"/>
    <dbReference type="eggNOG" id="COG1061">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013950_0_0_0"/>
    <dbReference type="OMA" id="NQEIAFK"/>
    <dbReference type="OrthoDB" id="13585at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="119"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="977" mass="115572" checksum="EAC952AF6A02BEF6" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>B7IER1</accession>
    <name>B7IER1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_67</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ74575">
        <property type="protein sequence ID" value="ACJ74575"/>
        <property type="gene ID" value="THA_67"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_67"/>
    <dbReference type="HOGENOM" id="CLU_3123686_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="50" mass="5583" checksum="493875993AABA555" modified="2009-02-10" version="1">MLIVTPGKIKGMADSILKVDFKYLFIFVLATIYAPGRPITVANIDEIKAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IER2</accession>
    <name>B7IER2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_68</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_68"/>
    <dbReference type="EnsemblBacteria" id="ACJ74576">
        <property type="protein sequence ID" value="ACJ74576"/>
        <property type="gene ID" value="THA_68"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_68"/>
    <dbReference type="eggNOG" id="COG1178">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021838_5_0_0"/>
    <dbReference type="OMA" id="PLYLFQL"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="289"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="229"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="418" mass="47302" checksum="AF38CA2FAFE47AC5" modified="2009-02-10" version="1">MSIGFLMTFGNNGIITNFLKLFGFNQRLLYTFSAIIIGHVFYNFPLFIRIVGETWEKIDSSIIESAKLDGANNLLIFFKIEIPLILSSILKAFLLTYVYTFTSFSVVLILGGIKYSTIEVAIYMYTKILFDFKSALTLSIVQIILISLISYILSFERHTFLSGKALKEKFPIWGYLYLLITTIFIFIPLIYSLLSGFIKYGGGFGIENFKLLFSLDLKRYIGTSFQSMIIFTLMFSISASLLTILISMIASFYNIRKKFKLEYIMLIPAAISPVTLAFSYIAIGISQFIAIPIIYSLISLPIVFGIISSGWKTLDEFSEEAALIDGANSFVLNFKIRIPQMKYHILTAFVYAFTLSLGEMSATITLSTPPVSTFSISIYRLLSSRKIPQARALNSIYSIIVLILFLIIEILRNKNKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IES4</accession>
    <name>B7IES4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_80</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CPA3 antiporters (TC 2.A.63) subunit F family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103430.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_80"/>
    <dbReference type="EnsemblBacteria" id="ACJ74588">
        <property type="protein sequence ID" value="ACJ74588"/>
        <property type="gene ID" value="THA_80"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_80"/>
    <dbReference type="HOGENOM" id="CLU_2604871_0_0_0"/>
    <dbReference type="OMA" id="PMLIDIG"/>
    <dbReference type="OrthoDB" id="2225447at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007208">
        <property type="entry name" value="MrpF/PhaF-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04066">
        <property type="entry name" value="MrpF_PhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="79" mass="9034" checksum="105DA471C854C6A7" modified="2009-02-10" version="1">MIYIILASIIFSIISVLKEKDIYYKLVPLLTIQTKISILIILYSYIKEQPMLIDIGIFYLLLSIGGTFVISSFISRSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IES6</accession>
    <name>B7IES6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF4040 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_82</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579339.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_82"/>
    <dbReference type="EnsemblBacteria" id="ACJ74590">
        <property type="protein sequence ID" value="ACJ74590"/>
        <property type="gene ID" value="THA_82"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_82"/>
    <dbReference type="eggNOG" id="COG1563">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1271776_0_0_0"/>
    <dbReference type="OMA" id="ILCENHQ"/>
    <dbReference type="OrthoDB" id="1678408at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025383">
        <property type="entry name" value="MbhD-like_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13244">
        <property type="entry name" value="DUF4040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="5"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4040" evidence="8">
        <location>
            <begin position="10"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="217" mass="25238" checksum="4336AC44B2949F2E" modified="2009-02-10" version="1">MIRYIFGMTAIFVTIFIFFSNKTFNSFLYRTILSVLMVAIYTLYFAPDVALAEAMLGALLSTFVYLLTFKIHSKIKVVIVEIPILCENHQNISVGLIPEILNDFAKKYNHKIEFIYEESISKVDKLLLSGEVDIAISYDGDFKILEIPIYKHKDRELTYFEITKSSDIDYSKVKKIKNASLYFKFSDKDTTLFEEFSSFYNSQYINEKLKKYKLGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEU0</accession>
    <name>B7IEU0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Keto/oxoacid ferredoxin oxidoreductase, gamma subunit, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_96</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579344.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_96"/>
    <dbReference type="EnsemblBacteria" id="ACJ74604">
        <property type="protein sequence ID" value="ACJ74604"/>
        <property type="gene ID" value="THA_96"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_96"/>
    <dbReference type="eggNOG" id="COG1014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_0_0_0"/>
    <dbReference type="OMA" id="KHSCLTQ"/>
    <dbReference type="OrthoDB" id="1777574at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="15"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="200" mass="22368" checksum="63FA2BA0A916CCFF" modified="2009-02-10" version="1">MEINRPFSIRIAGIGGQGNLVSGLILAKALVKSGKYVVQTQNYTAQVRGGPSYCDLLVLNQPIDFPKATLFDILIILHPSMISQCKNVRNNGIIIIDNTHISELPADMFRMTKRKIALPVSKMALEKFGNEMFANMIMLGVVLKATSLVDIDSLIESIKENINPKYVEKNIEAVKYGTTLTEKVYKPRIERKIKRTIGFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEX4</accession>
    <name>B7IEX4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
            <ecNumber evidence="3">1.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fabG</name>
        <name evidence="4" type="ordered locus">THA_130</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103556.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_130"/>
    <dbReference type="EnsemblBacteria" id="ACJ74638">
        <property type="protein sequence ID" value="ACJ74638"/>
        <property type="gene ID" value="THA_130"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_130"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_0"/>
    <dbReference type="OMA" id="KGAFYCA"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011284">
        <property type="entry name" value="3oxo_ACP_reduc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01830">
        <property type="entry name" value="3oxo_ACP_reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2 3" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="153"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU366074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="247" mass="26515" checksum="CA9C497FBD607528" modified="2009-02-10" version="1">MRLKDKVCIITGAASGIGKAAALLFSKEGATIIACDMSEENLKVLKEENPGPGVIDTYVLDVTDRKRINEVVEEVASKYGKIDVLVNNAGITRDALLLKMKEEDWDAVINVNLKGVFNMTQAVAPFMIKAGKGSIINTSSIVGVFGNIGQTNYSATKAGVIGMTKTWAKELARKGAQIRVNAVAPGFIKTPMTEKVPEKIINALNEKIPLKRMGEPEEVANLYLFLASDESSYITGQVIGIDGGLVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF42</accession>
    <name>FOLD_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional protein FolD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">Methylenetetrahydrofolate dehydrogenase</fullName>
                <ecNumber evidence="1">1.5.1.5</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Methenyltetrahydrofolate cyclohydrolase</fullName>
                <ecNumber evidence="1">3.5.4.9</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">folD</name>
        <name type="ordered locus">THA_201</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5,10-methenyltetrahydrofolate + H2O = (6S)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="1"/>
    <dbReference type="EC" id="3.5.4.9" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579409.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IF42"/>
    <dbReference type="SMR" id="B7IF42"/>
    <dbReference type="STRING" id="484019.THA_201"/>
    <dbReference type="PRIDE" id="B7IF42"/>
    <dbReference type="EnsemblBacteria" id="ACJ74706">
        <property type="protein sequence ID" value="ACJ74706"/>
        <property type="gene ID" value="THA_201"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_201"/>
    <dbReference type="eggNOG" id="COG0190">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034045_3_0_0"/>
    <dbReference type="OMA" id="HIVGRPM"/>
    <dbReference type="OrthoDB" id="1449514at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000147531" description="Bifunctional protein FolD">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="154"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01576"/>
        </source>
    </evidence>
    <sequence length="271" mass="29955" checksum="D7C16B023E7ED866" modified="2009-02-10" version="1">MLIDVKPLYSEIKNLIMDRMSKLKKVPKLVAVTYKPDPSTISYLKSQEKAAKRFGLDYEIFEASNSIEVLEKLRKLSEDKSVNGIFVTHPLPELDEMLVFENLSPEKDIEGRHPYNLGMLMYGNEFFAPCTAEAVVKILENVTEITGKNVVIIGRSTTVGKPLATMLLRRDRSATVTVCHTKTRNLPEITRNADIIVAAAGKARLVTKEMVKSDSIVIDVGINVVDGKIVGDVKEDVSEIAKVTPVPGGVGRITTALLMEHVVKSAEKMNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF62</accession>
    <name>B7IF62_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_221</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579425.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_221"/>
    <dbReference type="EnsemblBacteria" id="ACJ74726">
        <property type="protein sequence ID" value="ACJ74726"/>
        <property type="gene ID" value="THA_221"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_221"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_849752_0_0_0"/>
    <dbReference type="OMA" id="VEAYIEY"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="3">
        <location>
            <begin position="150"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="288" mass="34501" checksum="3EA49D8D15A88FCF" modified="2009-02-10" version="1">MIKLLIPQVLVESLREFLYEHREVVFDIYNSNLEEKIREDYWDIVYLTEEKTVPGKILVYSLRELELAVKLFEVKEEHRRLKEEYDMLYSFPELQGPIIREFLQKVILDFKEKNELVLLYEDGMYVNEYRTFFESRLPHTKVKFSKKKGVKIPPLRERKEDLPYIFDIVLSSLYLKHKNLDKKIPDNNEYELLKEYNWPGNTKELVKVASNYAATGRIEIPRFKASNFEGIDLVNFTSKLVKHVEKRYIMLALKKSNSRFEAAKMLKINYKTLSHKIKLYGIENSKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF81</accession>
    <name>B7IF81_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Magnesium transporter, CorA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_242</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103794.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_242"/>
    <dbReference type="EnsemblBacteria" id="ACJ74745">
        <property type="protein sequence ID" value="ACJ74745"/>
        <property type="gene ID" value="THA_242"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_242"/>
    <dbReference type="eggNOG" id="COG0598">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007127_8_1_0"/>
    <dbReference type="OMA" id="GILHTDH"/>
    <dbReference type="OrthoDB" id="822653at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="SSF143865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="SSF144083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="308" mass="35933" checksum="B6EFB00725006214" modified="2009-02-10" version="1">MVKFYTMEENKIVEVEKFVPNSLIKVIDPSADEIHMLSSLLQFDPDFIYDSLDDDERARIELDDDTVLIILKIPKKFNGNSKIPYRTVSMGVIIGKNYTIFSSKEPVDFIDYMIENNKFDINKKSRMIFQIFLLNAKVFLSYLKEVNKTISIIEEELHRSMKNKELEEMMYLEKSLVYFTTSLRSNEIMMEKLLRGNIIKIYEEDKDVLEDAIIENRQAIEVSNIYSDILAGMMDSYASVISNNLNIVMKILTVVTILIQVPTIITSFYGMNVKLPLQNNPIAYVHIIFWSIGTILLTLAWFKRKKWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF86</accession>
    <name>B7IF86_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isochorismatase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579441.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_248"/>
    <dbReference type="EnsemblBacteria" id="ACJ74750">
        <property type="protein sequence ID" value="ACJ74750"/>
        <property type="gene ID" value="THA_248"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_248"/>
    <dbReference type="eggNOG" id="COG1535">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_8_3_0"/>
    <dbReference type="OMA" id="GVMTDCC"/>
    <dbReference type="OrthoDB" id="1442962at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="188" mass="22141" checksum="3C175CEB89C74232" modified="2009-02-10" version="1">MNFYKERKKHPLVLNVPALLIVDVQSYFTDRESSAYLEGIENIYENIKKLSERFLQKGYPVISTIHKGGALQMQRWWGNKVDEKWAKPYFKSNEYVYIIKDTYDAFYNTYLEKVLRLKNVTDIIITGVMTHLCCETTARSGFVRNFNVVMVEDALWDKDEFYHFLSLKSLAHGFAHICKTEEVLCKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF89</accession>
    <name>B7IF89_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lipoprotein-releasing system ATP-binding protein LolD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_251</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579444.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_251"/>
    <dbReference type="EnsemblBacteria" id="ACJ74753">
        <property type="protein sequence ID" value="ACJ74753"/>
        <property type="gene ID" value="THA_251"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_251"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_0"/>
    <dbReference type="OMA" id="EVFCLIG"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="229" mass="25574" checksum="84FEFEDF273827F1" modified="2009-02-10" version="1">MIVRAKNLTKVYGKKEKFEALKGVNLEINEGEILAILGPSGCGKTTLLNCLSGLDEPTSGEVEVLGKRLDRMNEEEKTIFRSENMGFIFQFFNLIPVLTAVENVEIPLLISKNGTPSEIRKKALEWLERLHILHRKNAYPSEMSGGEQQRVAIARALINNPKIVWADEPTGALDTKNSQEIIDLIINMNKELGTTFVIVTHDINVAKKAHRIIKMDSGQIVSEEILVRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFA7</accession>
    <name>CH10_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Co-chaperonin GroES</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">10 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Chaperonin-10</fullName>
            <shortName evidence="1">Cpn10</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">groES</name>
        <name evidence="1" type="synonym">groS</name>
        <name type="ordered locus">THA_270</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heptamer of 7 subunits arranged in a ring. Interacts with the chaperonin GroEL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GroES chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579460.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFA7"/>
    <dbReference type="SMR" id="B7IFA7"/>
    <dbReference type="STRING" id="484019.THA_270"/>
    <dbReference type="EnsemblBacteria" id="ACJ74771">
        <property type="protein sequence ID" value="ACJ74771"/>
        <property type="gene ID" value="THA_270"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_270"/>
    <dbReference type="eggNOG" id="COG0234">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132825_2_0_0"/>
    <dbReference type="OMA" id="RSINMTI"/>
    <dbReference type="OrthoDB" id="1965002at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="PTHR10772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000129717" description="Co-chaperonin GroES">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00580"/>
        </source>
    </evidence>
    <sequence length="90" mass="10020" checksum="21F6195B33111315" modified="2009-02-10" version="1">MKVIPLGSRLLIKPIQEEKRTEGGIVLPDTAKEKPMKAEIVAVGNLEDADVDLHVGDKVIFSKYSGTEIKIEEEDYIIIDVEDILAKIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFB3</accession>
    <name>B7IFB3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Core-binding (CB) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_277</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579464.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_277"/>
    <dbReference type="EnsemblBacteria" id="ACJ74777">
        <property type="protein sequence ID" value="ACJ74777"/>
        <property type="gene ID" value="THA_277"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_277"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_762756_0_0_0"/>
    <dbReference type="OMA" id="EMINYLD"/>
    <dbReference type="OrthoDB" id="621410at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="4">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="363" mass="42402" checksum="A5B0A2FCA9D7B6F3" modified="2009-02-10" version="1">MNINSDLERFMEYINTQNISESTKKNYVNMVDSFLKFVEGNVTSENVRKWLSKIKENYKSTAWRQYFVPLRAFLQRFYPLIYSEVVEDLKVPYKKADYEMINYLDFWKLYEEAEKMAKHGNEKYVLLVGLAGILGLKSGDIVKLKGTDIRGNYLYVSDENYEVEIVPPIDKWLKKYEGKDEYLISTGDGEAVKPSYLALMLRGLQKKVDISLKRPLSVEILRNVAVARQVIAQPSLPYVMDLFKYSSTKTIEDIARYITESGDLRTILSIEVVDEMEPVLEFFNKLSIPVRKIENAYIVSRTAYIKRGDKFSRLNTLFVVRNLDGWQEYIEKLGYEVKRKPNDNFFIFLAGKLRVAFLELDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFB8</accession>
    <name>B7IFB8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="8">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">gltX1</name>
        <name evidence="8" type="synonym">gltX</name>
        <name evidence="12" type="ordered locus">THA_282</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579468.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_282"/>
    <dbReference type="EnsemblBacteria" id="ACJ74782">
        <property type="protein sequence ID" value="ACJ74782"/>
        <property type="gene ID" value="THA_282"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_282"/>
    <dbReference type="eggNOG" id="COG0008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015768_6_3_0"/>
    <dbReference type="OMA" id="WDEGPFF"/>
    <dbReference type="OrthoDB" id="1409413at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1c" evidence="10">
        <location>
            <begin position="2"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_2" evidence="11">
        <location>
            <begin position="340"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="250"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ74782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="473" mass="55059" checksum="BE3672829FE428EA" modified="2009-02-10" version="1">MVRLRFAPSPTGYLHVGGARTALFNFLYARKMNGKFILRIEDTDLERSEKEFEEGLIESMKWLGLNWDEGPDVGGGYGPYRQSERLDIYNKYAQKLVEEGKAYKVYAYPEEIEKLREELLSQGKAPHYTREMLESFTTPERIKEYEEKGLKPAIYFQMPRKEYVLNDIVKGQVVFKEGTLGDFAIIRSNGVPIYNFACVIDDYLMKITHVIRGDDHLSNTVKQLAIYEALGWNPPEFGHVSMILGPDAKKLSKRHGATSVEEFKERGYLPQAVVNFLALLGWSHPEGKEIMSLEELISAFSLDRLGKNPAIFDPQKLKWMNAEHFRHLSDEEMLEVSKPYLEKFAREEEINERKEWFIRLLKAIKDRVEELSQIPELVEFFFVEPENKVELSDEVKQVYSELVKEIENIEQWDEKNIYQAFKNAMKKGKVKGKDFYMNLRIVLTGREEGPELIDIVYLLGKENIVKRIQKQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFC9</accession>
    <name>B7IFC9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucose--hexose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">UDP-glucose--hexose-1-phosphate uridylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">galT1</name>
        <name evidence="16" type="ordered locus">THA_293</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:13989"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.7.7.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.12" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579477.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_293"/>
    <dbReference type="EnsemblBacteria" id="ACJ74793">
        <property type="protein sequence ID" value="ACJ74793"/>
        <property type="gene ID" value="THA_293"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_293"/>
    <dbReference type="eggNOG" id="COG1085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029960_1_0_0"/>
    <dbReference type="OMA" id="DWVMIAS"/>
    <dbReference type="OrthoDB" id="1064872at2"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00608">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001937">
        <property type="entry name" value="GalP_UDPtransf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005850">
        <property type="entry name" value="GalP_Utransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005849">
        <property type="entry name" value="GalP_Utransf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02744">
        <property type="entry name" value="GalP_UDP_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01087">
        <property type="entry name" value="GalP_UDP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000808">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00209">
        <property type="entry name" value="galT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GalP_UDP_transf" evidence="14">
        <location>
            <begin position="4"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="GalP_UDP_tr_C" evidence="15">
        <location>
            <begin position="189"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-UMP-histidine intermediate" evidence="13">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ACJ74793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="332" mass="38771" checksum="769DD08E5FA03AF6" modified="2009-02-10" version="1">MPEYRKDPVVRRWVIIATERAKRPHDFTVPKEEAKGGFCPFDYGNEHTTPPEIFAFRPENTEPNSPGWWVRVVPNKFPAVNPDIEIEKYGHGMYDAMAGFGYHEVVVETPEHSSTFALYDDKQAEEVVWAYVKRFRQLREDKRLKYILIFKNHGALGGASLPHPHSQIIAIPSVPKRVLEELNGAKDYYDYKERCVFCDMISQEKIENRRIVEENEDFIAFAPYASRFPFEVWIAPKVHSHDFGKIEDSQVKTFAIILRNTLKRIYKVLDNPPYNFVIHTSPTYEEGKIYYHWHVEIMPRLTRVAGFEWGSGFYINPVPPEDAAKYLKNVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFK8</accession>
    <name>B7IFK8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_379</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104080.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_379"/>
    <dbReference type="EnsemblBacteria" id="ACJ74872">
        <property type="protein sequence ID" value="ACJ74872"/>
        <property type="gene ID" value="THA_379"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_379"/>
    <dbReference type="eggNOG" id="ENOG50333I7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2304696_0_0_0"/>
    <dbReference type="OMA" id="FGSGCCL"/>
    <dbReference type="OrthoDB" id="1747471at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="100" mass="11883" checksum="603924D3D1DC98C0" modified="2009-02-10" version="1">MFFEDFDEEFESLDEMVEKLRNENSCPECVQCGYCCKVTPCYYGKWNEEKNQCEYLTEDNKCGIYDKIVEMEKDQEVKMFGSGCCLNYMNPERLKKLSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFQ4</accession>
    <name>B7IFQ4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MaoC family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579570.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_425"/>
    <dbReference type="EnsemblBacteria" id="ACJ74918">
        <property type="protein sequence ID" value="ACJ74918"/>
        <property type="gene ID" value="THA_425"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_425"/>
    <dbReference type="eggNOG" id="COG2030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_3_3_0"/>
    <dbReference type="OMA" id="PGPGCIY"/>
    <dbReference type="OrthoDB" id="1563802at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="13"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="136" mass="15112" checksum="3785431A63E023CB" modified="2009-02-10" version="1">MKYSDLKVGDTYEKSQVVEDQHVVSFAQITGDKNPVHLDEEYAKNSVFGGRIAHGILLLGYISAVLGMEFPGPGTIYMSQNAKFLKPVYIGEKIKIVIKIVEKDDNKKRAKLSTNIYKESGELAVEGEAMVSLKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFQ9</accession>
    <name>B7IFQ9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579575.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_430"/>
    <dbReference type="EnsemblBacteria" id="ACJ74923">
        <property type="protein sequence ID" value="ACJ74923"/>
        <property type="gene ID" value="THA_430"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_430"/>
    <dbReference type="HOGENOM" id="CLU_2332692_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="98" mass="11291" checksum="27C60B70896FE919" modified="2009-02-10" version="1">MKRLVLIVTILIAISCFSDDKLLHMNVSSFLESIFSSFSDKSEIITFSIGIAKEVYDLLSKNGVCDFYDILADIYGIMLYKMYKFKDYKFSIVIALEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFS4</accession>
    <name>B7IFS4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heptaprenyl diphosphate synthase component I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_447"/>
    <dbReference type="EnsemblBacteria" id="ACJ74938">
        <property type="protein sequence ID" value="ACJ74938"/>
        <property type="gene ID" value="THA_447"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_447"/>
    <dbReference type="eggNOG" id="COG4769">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108933_0_0_0"/>
    <dbReference type="OMA" id="PIPYFRI"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014535">
        <property type="entry name" value="Hpre_diP_synt_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010898">
        <property type="entry name" value="Hpre_diP_synth_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07456">
        <property type="entry name" value="Hpre_diP_synt_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027391">
        <property type="entry name" value="Hpre_diP_synt_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="154" mass="17100" checksum="D6E39C317C73F8FF" modified="2009-02-10" version="1">MFVVERFIPYPVPGGKWGFSNFVILYTVVNIGLRGGLLVGTLKTIIGSLFSGILFTPPFFMGFFGIISAATFEWIFSKTKFFSYTTLSIIGMISNNFVQVLVGSFLIKSRAIFSFLPLMIGLGLISAIINAYLAKKMEEIIDENNFGLKIPKKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFV8</accession>
    <name>FLIW_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flagellar assembly factor FliW</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fliW</name>
        <name type="ordered locus">THA_481</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as an anti-CsrA protein, binds CsrA and prevents it from repressing translation of its target genes, one of which is flagellin. Binds to flagellin and participates in the assembly of the flagellum.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with translational regulator CsrA and flagellin(s).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FliW family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579608.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFV8"/>
    <dbReference type="SMR" id="B7IFV8"/>
    <dbReference type="STRING" id="484019.THA_481"/>
    <dbReference type="EnsemblBacteria" id="ACJ74972">
        <property type="protein sequence ID" value="ACJ74972"/>
        <property type="gene ID" value="THA_481"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_481"/>
    <dbReference type="eggNOG" id="COG1699">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112356_0_2_0"/>
    <dbReference type="OMA" id="DVAVFCI"/>
    <dbReference type="OrthoDB" id="1767625at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01185">
        <property type="entry name" value="FliW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003775">
        <property type="entry name" value="Flagellar_assembly_factor_FliW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024046">
        <property type="entry name" value="Flagellar_assmbl_FliW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39190">
        <property type="entry name" value="PTHR39190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02623">
        <property type="entry name" value="FliW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141457">
        <property type="entry name" value="SSF141457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <feature type="chain" id="PRO_1000138262" description="Flagellar assembly factor FliW">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01185"/>
        </source>
    </evidence>
    <sequence length="145" mass="16833" checksum="1D3B2BF5A91F2313" modified="2009-02-10" version="1">MKYNTRLGEIEINENEIITFEKGIPGFEHLRKFSVISLKDTLPILWLVSLEDENVSLPIIDPWIVDKNYEIEISNEDIKELEIEDKEKVAVWAILTIPSGHPEETTVNLRAPIVINMEKGKGKQIILDTEKYKIKHKLSEFSFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFZ8</accession>
    <name>B7IFZ8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_523</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100650.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_523"/>
    <dbReference type="EnsemblBacteria" id="ACJ75012">
        <property type="protein sequence ID" value="ACJ75012"/>
        <property type="gene ID" value="THA_523"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_523"/>
    <dbReference type="eggNOG" id="COG3330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1065321_0_0_0"/>
    <dbReference type="OMA" id="SSRERWY"/>
    <dbReference type="OrthoDB" id="1711249at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032585">
        <property type="entry name" value="DUF4912"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16258">
        <property type="entry name" value="DUF4912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="261" mass="30407" checksum="681C99FFBB6FAE06" modified="2009-02-10" version="1">MFDGNIEKLKKWLDSNPTIQELKSQARILGLKVKRMMKKREVIKLIEGYINQMQELQEIQRPSSANSVNNVKEDQDIKQEKDIKLPESYNKNKLVVLPVNPNWLHVYWDLSDKTRESLMNLPNGFRIVLRIYDVTFIDFNGNNAHRTFEISVDLRTMKNYYVNVPMPNADYLAELGYVAPDGEFHPIIRSNLCKTPPNSQSQSTRERWLDIRKKRKIVTPSDGVLVKEVETIPGSIFNNNPLSKEESIWQLFSRLRSGRGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG00</accession>
    <name>B7IG00_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-serine dehydratase</fullName>
            <ecNumber evidence="8">4.3.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">sdaB</name>
        <name evidence="10" type="ordered locus">THA_525</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>L-serine = NH4(+) + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:19169"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="EC" id="4.3.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the iron-sulfur dependent L-serine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.17" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100646.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_525"/>
    <dbReference type="EnsemblBacteria" id="ACJ75014">
        <property type="protein sequence ID" value="ACJ75014"/>
        <property type="gene ID" value="THA_525"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_525"/>
    <dbReference type="eggNOG" id="COG1760">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086592_0_0_0"/>
    <dbReference type="OMA" id="HTAGACR"/>
    <dbReference type="OrthoDB" id="1720141at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029009">
        <property type="entry name" value="ASB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004643">
        <property type="entry name" value="Fe-S_L-Ser_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005131">
        <property type="entry name" value="Ser_deHydtase_bsu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03315">
        <property type="entry name" value="SDH_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036692">
        <property type="entry name" value="SDH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143548">
        <property type="entry name" value="SSF143548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00719">
        <property type="entry name" value="sda_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SDH_beta" evidence="9">
        <location>
            <begin position="10"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="215" mass="24044" checksum="E5BB067C4B2D32B1" modified="2009-02-10" version="1">MSLLKILGPVMVGPSSSHTLGALKISRFAYKLVGKTPDRVTFLLHGSFSKTYKGHGTDRALLAGIMGFKQDDPRIKDAFEIAKERGLKYSFEIQDLGEVHPNTVRIKFEVDGISHEIEGSSIGGGDIKITNIDSVPCDLSWDYDTLVIVNKDIPKALEKILDAINVNVANLYLRRINALLERALTIVELDEPIDNLEEITKIPYVYECYFIGRDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG03</accession>
    <name>B7IG03_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_529</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579632.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_529"/>
    <dbReference type="EnsemblBacteria" id="ACJ75017">
        <property type="protein sequence ID" value="ACJ75017"/>
        <property type="gene ID" value="THA_529"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_529"/>
    <dbReference type="eggNOG" id="ENOG50337V2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1314881_0_0_0"/>
    <dbReference type="OMA" id="HLFGDYV"/>
    <dbReference type="OrthoDB" id="1497255at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021737">
        <property type="entry name" value="Phage_phiKZ_Orf197"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11750">
        <property type="entry name" value="DUF3307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="210" mass="24702" checksum="F6CA79EAA3062BD5" modified="2009-02-10" version="1">MDRFIHLFLGHLVGDYVLQNKFIASYKQKKLKVLLAHIILIFFSQVAFFAGKDFNINSIYIISFVTLSHFFIDFFKYKNNNKPFFKSPKYYLIDQLMHIFTLFLASIFSTQTFFYIPNKLAVILISAIFNAYFLGIYTFILKNNVGEYKRDFVGYIVRGLCPIFYIFGPLVYSVYTLLTGFFSLYFLKSYQVISWALSIISTIIFMEVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG34</accession>
    <name>B7IG34_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative Holliday junction resolvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579649.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_560"/>
    <dbReference type="EnsemblBacteria" id="ACJ75048">
        <property type="protein sequence ID" value="ACJ75048"/>
        <property type="gene ID" value="THA_560"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_560"/>
    <dbReference type="eggNOG" id="COG0816">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108858_0_0_0"/>
    <dbReference type="OMA" id="SGKECEQ"/>
    <dbReference type="OrthoDB" id="1908834at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005227">
        <property type="entry name" value="YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03652">
        <property type="entry name" value="RuvX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="YqgFc" evidence="2">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="202" mass="22671" checksum="E4444D46A0E0E0D1" modified="2009-02-10" version="1">MIIAIDYGNSKCGYAIGNNFISKSGAVKTKDIMKLISNAKKIVMGIPLSMSGNYSTQSLKVLTFANNLVEKGYDVFLIDERLTTKMASSFGIKDDDAFSARQIFMDFVQNPSVAQKFRKEKFLNLELREENALFYEVPPSKNIKADALTKDYSIAFLHLSIGNFTYSNPDTLDKKYNIVITKKEFEKNGKNFLKSGGKIILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG43</accession>
    <name>B7IG43_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP synthase gamma chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP synthase F1 sector gamma subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">F-ATPase gamma subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">atpG</name>
        <name evidence="10" type="ordered locus">THA_570</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579654.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_570"/>
    <dbReference type="EnsemblBacteria" id="ACJ75057">
        <property type="protein sequence ID" value="ACJ75057"/>
        <property type="gene ID" value="THA_570"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_570"/>
    <dbReference type="eggNOG" id="COG0224">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050669_0_1_0"/>
    <dbReference type="OMA" id="MQITSAM"/>
    <dbReference type="OrthoDB" id="1701531at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00815">
        <property type="entry name" value="ATP_synth_gamma_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="PTHR11693"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="SSF52943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0139">CF(1)</keyword>
    <keyword evidence="4 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="273" mass="31193" checksum="3D9211E934E90679" modified="2009-02-10" version="1">MSRGKLLSIKKRVQSTESLKKITKAMEMVATARVKKVEKNLKGVREFLNETKRILENIEFVGNHPFITGEGKKALVVISTDMGLCGAFPTEIGRAALNFVEKEGIDYVYAIGNKTIPFFKKHKSTRRIYERIYDVPTFDFAKTLTNDLVNDGVGKIFVVYGKFKNRLMQKPEIVQLTPIEIEKTKSSRYEYEPELENLVDSALVFYVATSIYSYAYETKVSELYSRQNAMRNATENADEVIRELTLEFNKERQASITQELIEIVSGAQALQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG47</accession>
    <name>B7IG47_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_574"/>
    <dbReference type="EnsemblBacteria" id="ACJ75061">
        <property type="protein sequence ID" value="ACJ75061"/>
        <property type="gene ID" value="THA_574"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_574"/>
    <dbReference type="eggNOG" id="ENOG502ZGRB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1685742_0_0_0"/>
    <dbReference type="OMA" id="FYTMELV"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="168" mass="19184" checksum="1D3590A70FD2F37B" modified="2009-02-10" version="1">MKGGNGGINLHIMEKNLSKLFSASLPGDDLVAFVSVFFSLGFAANFFFSFTNKNIYISNTIFLVGILGYLLIAYKWCSSLPEFLKNNVLIFYNYVILSILFCFLSYFFPLLFLISGSLVISLIIAIDKKIHQSNIFLKIMFILLTLTFYLLELAPDVNFRLRNLKEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG68</accession>
    <name>B7IG68_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-dependent DNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579669.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_595"/>
    <dbReference type="EnsemblBacteria" id="ACJ75082">
        <property type="protein sequence ID" value="ACJ75082"/>
        <property type="gene ID" value="THA_595"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_595"/>
    <dbReference type="eggNOG" id="COG2865">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024970_5_2_0"/>
    <dbReference type="OMA" id="AILHIMY"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025831">
        <property type="entry name" value="RecG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038475">
        <property type="entry name" value="RecG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13749">
        <property type="entry name" value="HATPase_c_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HATPase_c_4" evidence="1">
        <location>
            <begin position="13"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="174" mass="20169" checksum="E676FBC10FB60F21" modified="2009-02-10" version="1">MNAVVHRDYSFKGSDIKLAIFDDRVEITSPGGLPGNLSVDLILQGRSEIRNRVIARFFKEIGYVEQWGTGIKRIVELCKARNLKEPEFIDDGAFFKVIIYKVPDSAGYENKILDFLYKNGKITRVDVEQLFNVKERRARDILKNMVDKGILKRKGKGSKVYYELNKDRDFFIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG98</accession>
    <name>B7IG98_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sensory box histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_626"/>
    <dbReference type="EnsemblBacteria" id="ACJ75112">
        <property type="protein sequence ID" value="ACJ75112"/>
        <property type="gene ID" value="THA_626"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_626"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_32_0"/>
    <dbReference type="OMA" id="GTFCVMD"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="212"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="331" mass="39352" checksum="82301A3CE6A69249" modified="2009-02-10" version="1">MRNLRVLKKVGCFDFSCKKSFEKNFLYRVYKIFNKYDDKDDTIDTLKEIFEFIAEILEAQAWSLLLTPKEKKWRFIVWNYNLDDKPLEVLGELFQNNTPYNLSQIISKKRYYFVSDVLKTKYWKEVKGIDTRSWCGIPLVYNGEVYAILNVDWYRKKRLKKIDKILFDIITEELSKPILNILRIKERLEEKNIDLLTGLYNRGVLDYIDNSKYNYLIFLDLDNFKEVNDSFGHLVGDEVLRIISRRLKNTVKSDDLIIRYGGDEFIILVNSTREGLEKLIQRIKEHVSRNINVEDKDVNVGVSIGYCMLDKGFEDAIRIADEMMYKDKNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGD6</accession>
    <name>B7IGD6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vitamin B12-dependent ribonucleotide reductase</fullName>
            <ecNumber evidence="11">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">THA_675</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Genetic information processing; DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the ribonucleoside diphosphate reductase class-2 family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="11"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579719.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_675"/>
    <dbReference type="EnsemblBacteria" id="ACJ75150">
        <property type="protein sequence ID" value="ACJ75150"/>
        <property type="gene ID" value="THA_675"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_675"/>
    <dbReference type="eggNOG" id="COG0209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000404_2_0_0"/>
    <dbReference type="OMA" id="MMATMRC"/>
    <dbReference type="OrthoDB" id="357568at2"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02888">
        <property type="entry name" value="RNR_II_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013344">
        <property type="entry name" value="RNR_NrdJ/NrdZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="SSF48168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02504">
        <property type="entry name" value="NrdJ_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="10 11" id="KW-0170">Cobalt</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 11" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonuc_red_lgN" evidence="12">
        <location>
            <begin position="14"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="RIBORED_LARGE" evidence="13">
        <location>
            <begin position="135"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ75150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="838" mass="95029" checksum="658A42801A7B6557" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGE5</accession>
    <name>B7IGE5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Maltose ABC transporter, periplasmic maltose-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_687</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579728.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_687"/>
    <dbReference type="EnsemblBacteria" id="ACJ75159">
        <property type="protein sequence ID" value="ACJ75159"/>
        <property type="gene ID" value="THA_687"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_687"/>
    <dbReference type="eggNOG" id="COG2182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_17_0_0"/>
    <dbReference type="OMA" id="LWVDTEQ"/>
    <dbReference type="OrthoDB" id="1303718at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015144">
        <property type="term" value="F:carbohydrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006060">
        <property type="entry name" value="Maltose/Cyclodextrin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00181">
        <property type="entry name" value="MALTOSEBP"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="391" mass="42996" checksum="61A1FB44D59AF3DA" modified="2009-02-10" version="1">MKKLLVALLMVVMVFSVFAVKITIWTSEAQAPILTKLADEFKSIYGIDVEVVQVNFNDIKSKFLTAAPAGEGADIIVGAHDWVGELAANGLLEPIPVLPEKDQYLPTPLQAFTYNGKLYGIPYAFDGPALIYNKDYVEEPPKTFDELIELAKQIQEEYEGEVRGLVYDFKNFYFSSYAIFGFGGYVFGEKDGKINVKDVGLANEGAIKGMKLLKRLVDEGLLESGDNYNVMDNLFKDGQAAMIINGPWAVPGWKEAGIDFGVAPIPDLENGVKPKPFFGAQGFMVNAKSPNKLYAIEFLTKFIATKDVMYRIYLADPRVPSRKDILPMVDDVTRAFAEFLGNYGLPMPNVPEMAGVWGAMGDDALSKVLDQGVPVEQALKEAVDTILAGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGG8</accession>
    <name>B7IGG8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Chaperone ClpB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_711</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579746.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_711"/>
    <dbReference type="EnsemblBacteria" id="ACJ75182">
        <property type="protein sequence ID" value="ACJ75182"/>
        <property type="gene ID" value="THA_711"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_711"/>
    <dbReference type="eggNOG" id="COG0542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005070_4_1_0"/>
    <dbReference type="OMA" id="SKMMQGE"/>
    <dbReference type="OrthoDB" id="44062at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clp R" evidence="8">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="403"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="697"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="788" mass="89190" checksum="DCF059B613F7E777" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGI9</accession>
    <name>B7IGI9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="3">2.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5'-phosphoribosylglycinamide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">GAR transformylase</fullName>
            <shortName evidence="3">GART</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">purN</name>
        <name evidence="5" type="ordered locus">THA_737</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the transfer of a formyl group from 10-formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6S)-10-formyltetrahydrofolate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide</text>
            <dbReference type="Rhea" id="RHEA:15053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="EC" id="2.1.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GART family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579763.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_737"/>
    <dbReference type="EnsemblBacteria" id="ACJ75203">
        <property type="protein sequence ID" value="ACJ75203"/>
        <property type="gene ID" value="THA_737"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_737"/>
    <dbReference type="eggNOG" id="COG0299">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038395_1_3_0"/>
    <dbReference type="OMA" id="TGITIHY"/>
    <dbReference type="OrthoDB" id="1815747at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00126"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08645">
        <property type="entry name" value="FMT_core_GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01930">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004607">
        <property type="entry name" value="GART"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00639">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl_trans_N" evidence="4">
        <location>
            <begin position="11"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="5'-phosphoribosylglycinamide binding" evidence="3">
        <location>
            <begin position="20"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="10-formyltetrahydrofolate binding" evidence="3">
        <location>
            <begin position="90"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>(6S)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
        </ligand>
    </feature>
    <feature type="site" description="Raises pKa of active site His" evidence="3">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="185" mass="20829" checksum="26C230934DF757D7" modified="2009-02-10" version="1">MPGMSQSKLPRIVVLASGNGSNFEAIVKAQREGKLRAEILMLVVNKECFAIERAKRLGISYKKLSKDWKGELFALLEELSPDLVVLAGFMKILPPNIVNKWKIVNIHPSLLPAFKGKDAIKQAYEYGVKVTGITIHYVDEGVDTGPIIFQHAINIDGMSFEEVEEEIHKIEHKYYPIIIDKILND</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGJ4</accession>
    <name>B7IGJ4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase, group 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579767.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_742"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75208">
        <property type="protein sequence ID" value="ACJ75208"/>
        <property type="gene ID" value="THA_742"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_742"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_4_0"/>
    <dbReference type="OMA" id="CHGSDIN"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="1">
        <location>
            <begin position="82"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="400" mass="45600" checksum="1AB55442E4C0AFD2" modified="2009-02-10" version="1">MKPLILTNLFPRKDNPNSGIFITKRLKEYQKLGVDFTAVSLAFRDKGRLLNLLRSLLHKPSEIPLEELEGVSFNPVFVERWLFDVAVQKLWTMKKALENFTDRFAEKISREFPKHNIIHAHGMYLPAPAGVVARKVSEIWNVPYVVTLHGSDVNLNMARRDMRNIYLETLENAARCIFVSRALLEKAKSFGYSGQNAVVIPNGYDPEIFKPIDKDTVRKELGIYKENTHYVGFVGNLIPIKRADKLPEIFGKISKELPNSRFIIVGDGTLRDKILKEMEGLDVVFAGRVPQVKVARYMNAMDVMVLPSRNEGWPCVVLEAQACGTCVIGSSNGGIPEAIGFDEYVVEEGESFEDRFAKRVVEVLKKGYNKNILIERAKSFNWEKTVKREIEVYYQAPWPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGL7</accession>
    <name>B7IGL7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_766</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579787.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_766"/>
    <dbReference type="EnsemblBacteria" id="ACJ75231">
        <property type="protein sequence ID" value="ACJ75231"/>
        <property type="gene ID" value="THA_766"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_766"/>
    <dbReference type="eggNOG" id="COG4948">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1509917_0_0_0"/>
    <dbReference type="OMA" id="FPNHPKV"/>
    <dbReference type="OrthoDB" id="1269116at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="179" mass="21012" checksum="18926DAD8941FCDD" modified="2009-02-10" version="1">MKFFYNLKKADFGEYVVIEITDDKNIGIGAIIPERSKGENYKTIMGAIEEYRYIVEKANIEDAFEIPYKLEKYFPNHPKVIFAIDAAFKELYSKTYNISLVKLIGQENIQECKEPLEQEKVFPEEYGFIDIVKVLPKVYLEDSTFVLTKYPEGEMFEVLKALSTNYKYVEVLSYKDRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGS5</accession>
    <name>B7IGS5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="6">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="6">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">coaD</name>
        <name evidence="8" type="synonym">coaD1</name>
        <name evidence="8" type="ordered locus">THA_827</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the bacterial CoaD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579820.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_827"/>
    <dbReference type="EnsemblBacteria" id="ACJ75289">
        <property type="protein sequence ID" value="ACJ75289"/>
        <property type="gene ID" value="THA_827"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_827"/>
    <dbReference type="eggNOG" id="COG0669">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100149_0_1_0"/>
    <dbReference type="OMA" id="EDVSAFC"/>
    <dbReference type="OrthoDB" id="1846503at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00355"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02163">
        <property type="entry name" value="PPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00151">
        <property type="entry name" value="PPAT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001980">
        <property type="entry name" value="PPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF1">
        <property type="entry name" value="PTHR21342:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01020">
        <property type="entry name" value="LPSBIOSNTHSS"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01510">
        <property type="entry name" value="coaD_prev_kdtB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="7">
        <location>
            <begin position="4"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="8"/>
            <end position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="87"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="122"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="165" mass="19074" checksum="5AAA3B81E6C5D3AA" modified="2009-02-10" version="1">MKAIYPGSFDPITLGHLDIIERASKLFSEIYIVVMENKRKKYTFSLDERIEMIRECTGHIDNLKIDFFRGLLIEYVKNNNIDVVIRGLRAVTDFEYELQMAMANKEMCPNVDTVFLMTDKKYSFISSSLVKEVAYYGGDISRWVPKNVEKKLITLLSKNKGGIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGT5</accession>
    <name>B7IGT5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_837</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579825.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_837"/>
    <dbReference type="EnsemblBacteria" id="ACJ75299">
        <property type="protein sequence ID" value="ACJ75299"/>
        <property type="gene ID" value="THA_837"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_837"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_45_0_0"/>
    <dbReference type="OMA" id="ALKFLMW"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="192" mass="23391" checksum="CFD08631851E1158" modified="2009-02-10" version="1">MTKKEKIIKYALKEFAEKGFLAASTNTISKASKVSKGAIFYHFKNKENLYIECFKKIQGEYEKEFLKFYSKNTHLDFFEFLIKWSYKKIEIAKEKPEYLQFFKTVESIPESLKEKIFKLNQEIYSKYIPYFSEKFEKLELKENMDRNFSFKITFEILEYFSKKYYYEFESNLEKIMEEIEKVINIIKFGILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGT9</accession>
    <name>B7IGT9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ROK family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_842</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100463.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_842"/>
    <dbReference type="EnsemblBacteria" id="ACJ75303">
        <property type="protein sequence ID" value="ACJ75303"/>
        <property type="gene ID" value="THA_842"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_842"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_13_5_0"/>
    <dbReference type="OMA" id="QTGCLEY"/>
    <dbReference type="OrthoDB" id="1130699at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="3">
        <location>
            <begin position="10"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="373" mass="42811" checksum="EF6AB80D7773B9D1" modified="2009-02-10" version="1">MKLNNSKKILLNLLFKNEAYRNQLQKSLKVTPSTLSYILDNLKKKGYITVLKSESETVGRPKHIIKMDKNFWKSIGIRIGRESVNLTVFNGYFEEEEKISIKLTKNDIGNENISKILEKIIKKVSSLDKIKAIGIALSGNIDKDKANSKILKLENFNPHYIIKKLLPNTIVSLLNDVEAIATEEFVKHGGKKILVINYGTGIGACFYESRGIYEKSERKIIELGHFYVGTNEKCYCGATGCLETIASDYANLKRYKFSNLKIEDFILNEELYNNDLDELRNLHKTYEKKAQEIYNESFKYLAIFLTNIFKLFNPEKIILSGEGVTKWFSNELERKIHSISNFPVSITYRGLKNNIEFGAAVDALREYILRSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGY6</accession>
    <name>B7IGY6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DZANK-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_891</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579865.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75350">
        <property type="protein sequence ID" value="ACJ75350"/>
        <property type="gene ID" value="THA_891"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_891"/>
    <dbReference type="eggNOG" id="COG1933">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2439784_0_0_0"/>
    <dbReference type="OrthoDB" id="2085023at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025874">
        <property type="entry name" value="DZR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12773">
        <property type="entry name" value="DZR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DZANK-type" evidence="4">
        <location>
            <begin position="8"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="90" mass="10969" checksum="2C71551B397D2AB2" modified="2009-02-10" version="1">MYQGWKECPRCGIEVKNNEDYCPECGWVFYNKRCPRCGKYIPDYAKVCFDCGWYFYWEKEIGEDDEDTMNTIAMNIMKRMMNQWKIIQIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH19</accession>
    <name>B7IH19_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isoamylase N-domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579882.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_924"/>
    <dbReference type="CAZy" id="CBM48">
        <property type="family name" value="Carbohydrate-Binding Module Family 48"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75383">
        <property type="protein sequence ID" value="ACJ75383"/>
        <property type="gene ID" value="THA_924"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_924"/>
    <dbReference type="eggNOG" id="COG0296">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1151358_0_0_0"/>
    <dbReference type="OrthoDB" id="271838at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045859">
        <property type="term" value="P:regulation of protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030077">
        <property type="entry name" value="SIP2/GAL83"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF84">
        <property type="entry name" value="PTHR10343:SF84"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMPK1_CBM" evidence="1">
        <location>
            <begin position="27"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="AMPK1_CBM" evidence="1">
        <location>
            <begin position="148"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="241" mass="27354" checksum="8EBC197BE5F00DEB" modified="2009-02-10" version="1">MKKFFIVIILVLSLVSFSKVFVEDGKVVFTFNEALDAKVVYLAGTFNNWSPNTLAMEKVDGVWRVSLELEPGTYQYKYVIEGKNWKEDSEAPGYVDDGFGGKNGIFTLVDKDGTLVVLQEKVEKPALRINENFDPEMMFVDEEGYVVIRFKYDGQADYVTIAGSFNNWDAQDIECYEIDDGLWEAVLELDEGQYQYKFVVNGSEWVVDENAPAFVDDGFGGENGYFEVFKENGKLMVGLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH31</accession>
    <name>B7IH31_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_936</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100837.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_936"/>
    <dbReference type="EnsemblBacteria" id="ACJ75395">
        <property type="protein sequence ID" value="ACJ75395"/>
        <property type="gene ID" value="THA_936"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_936"/>
    <dbReference type="HOGENOM" id="CLU_1045585_0_0_0"/>
    <dbReference type="OMA" id="RNENEAW"/>
    <dbReference type="OrthoDB" id="1657970at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="265" mass="30448" checksum="9D23636E3537E160" modified="2009-02-10" version="1">MERMYVLIGISAILIAGLLFLIFPKTSVIEFSGVVLREIPKNSIIVEKDLAVAERIKKLYEEGNLFVFEGSVTLPQRNENEAWQQAANKARQELASFLGTKITSDSSLNEKIFGVRSAFGYEQDVSVVVNNFVVSSKVIAKWKVPVKNGFFEYHVLVFYDPDLIESVSKKQQQSMQLYFVVYDVKKGRVVKIERVDDIARYKEKFEFARKNGKVVIFEVRNKKIIAKSDIEALRIVKNANFEDGRYRLLYVKNGDYIYAFILKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH60</accession>
    <name>B7IH60_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulatory protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_965</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579906.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_965"/>
    <dbReference type="EnsemblBacteria" id="ACJ75424">
        <property type="protein sequence ID" value="ACJ75424"/>
        <property type="gene ID" value="THA_965"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_965"/>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_43_6_0"/>
    <dbReference type="OMA" id="NAGHNPV"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="241"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="457" mass="51644" checksum="4568E255B0A5BB2D" modified="2009-02-10" version="1">MIKRLQEIYERLCEISGKESKIFESEEKLLNAIEEEIEKINAEILTYKQELESLTILIESQLEEITRLYEEISTLFEISKIIASNIETKQILRPILYTLKKAIGFKCGIISLNFDGKVVETVGTCPENIDQLLSENAKEVSSETVIKERCEKLNGESIIFKYISTTSDEKSGYLLFVGKESGSIFTAGDKKIVESTAQQISGSIEREIALKEEIERERLNQQIEIARNIQLNFFPKKFPHDDNFDSFGESTPAIHVGGDYFDIFVKNDFLFGIVADVSGKGLPASLIMSSMRGAFKSLIESTDGDLKRTFNSLNNMVSVDVGEDRFVTCVSFKISRDGVLEVINAGHVPLYIVNSSSISKVESTNTPLGMFEDMDFEVQTFKLSSGDLIFAYTDGIPEARNLSGEEYDFERLERILKKVYLLSAKEIIYNVENDVFRFSQGAPQHDDMTLLAIKYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH64</accession>
    <name>B7IH64_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lipopolysaccharide export system ATP-binding protein LptB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">THA_969</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the ABC transporter complex LptBFG involved in the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane. Probably responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the lipopolysaccharide transport and assembly complex. The LptBFG transporter is composed of two ATP-binding proteins (LptB) and two transmembrane proteins (LptF and LptG).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABC transporter superfamily. Outer membrane lipopolysaccharide export (TC 1.B.42) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579908.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_969"/>
    <dbReference type="EnsemblBacteria" id="ACJ75428">
        <property type="protein sequence ID" value="ACJ75428"/>
        <property type="gene ID" value="THA_969"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_969"/>
    <dbReference type="eggNOG" id="COG1137">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_0"/>
    <dbReference type="OMA" id="LPMYQRA"/>
    <dbReference type="OrthoDB" id="1220708at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030921">
        <property type="entry name" value="LPS_export_LptB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04406">
        <property type="entry name" value="LPS_export_lptB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="3"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ75428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="231" mass="25889" checksum="E6B70DD6D47A94E3" modified="2009-02-10" version="1">MEIVCKDVKKRFGRKQVLNGINLNVRTGEVVGLLGPNGSGKTTLFNIILGVVIPSSGKIYLGEKDITKIPIHKRAKLGITYLQQETSVFRQLSVEDNLKLVIEYHDKDVKKIPKLLKEFGIDSLKDQYAFYLSGGEKRRLELARMMTLSPKFLLLDEPFVGIDPKTVKEIQKMVISLKERGLGIIITDHSVDALKDIVDRLYVIHKGDTIASGQPEEVLNDETVKEVYLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHD2</accession>
    <name>B7IHD2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alkaline protease A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1038</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579949.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1038"/>
    <dbReference type="EnsemblBacteria" id="ACJ75496">
        <property type="protein sequence ID" value="ACJ75496"/>
        <property type="gene ID" value="THA_1038"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1038"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_15_6_0"/>
    <dbReference type="OMA" id="YQWHYEA"/>
    <dbReference type="OrthoDB" id="923655at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07484">
        <property type="entry name" value="Peptidases_S8_Thermitase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034084">
        <property type="entry name" value="Thermitase-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase_S8" evidence="6">
        <location>
            <begin position="183"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="560" mass="60376" checksum="31F78C808A41A5FA" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHD9</accession>
    <name>B7IHD9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Adenosylcobinamide kinase</fullName>
            <ecNumber evidence="8">2.7.1.156</ecNumber>
            <ecNumber evidence="9">2.7.7.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Adenosylcobinamide-phosphate guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">THA_1045</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes ATP-dependent phosphorylation of adenosylcobinamide and addition of GMP to adenosylcobinamide phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosylcob(III)inamide + ATP = adenosylcob(III)inamide phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15769"/>
            <dbReference type="ChEBI" id="CHEBI:2480"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.156"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosylcob(III)inamide + GTP = adenosylcob(III)inamide phosphate + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15765"/>
            <dbReference type="ChEBI" id="CHEBI:2480"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="EC" id="2.7.1.156"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>adenosylcob(III)inamide phosphate + GTP + H(+) = adenosylcob(III)inamide-GDP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22712"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.7.62"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 5/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 6/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CobU/CobP family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.156" evidence="8"/>
    <dbReference type="EC" id="2.7.7.62" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579954.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1045"/>
    <dbReference type="EnsemblBacteria" id="ACJ75503">
        <property type="protein sequence ID" value="ACJ75503"/>
        <property type="gene ID" value="THA_1045"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1045"/>
    <dbReference type="eggNOG" id="COG2087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094161_0_1_0"/>
    <dbReference type="OMA" id="DCLTVWL"/>
    <dbReference type="OrthoDB" id="1903913at2"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00236"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043752">
        <property type="term" value="F:adenosylcobinamide kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008820">
        <property type="term" value="F:cobinamide phosphate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003203">
        <property type="entry name" value="Cobinamide_kinase/P_G-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34848">
        <property type="entry name" value="PTHR34848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02283">
        <property type="entry name" value="CobU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006135">
        <property type="entry name" value="CobU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="GMP-histidine intermediate" evidence="16">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="31"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="48"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00003889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00007490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR006135-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR006135-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ACJ75503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="165" mass="18936" checksum="AB54A4A0A7D70F12" modified="2009-02-10" version="1">MITLITGGVKSGKSSFALKLSENFKKKAFIATGVAFDEEMKKRIEKHKQERIGFDTFEEPIEVYKIIESLNQKYEVAILDCLTTYLGNLYYYEKDIEYYTKKLIDSLKRVNYNLIIVTNEVGWGIIPENKLSRKYVDNLGLLNKKVASLSDNVYLMVSGIGVKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHF8</accession>
    <name>B7IHF8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sirohydrochlorin cobaltochelatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbiX</name>
        <name evidence="3" type="ordered locus">THA_1066</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579973.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1066"/>
    <dbReference type="EnsemblBacteria" id="ACJ75522">
        <property type="protein sequence ID" value="ACJ75522"/>
        <property type="gene ID" value="THA_1066"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1066"/>
    <dbReference type="eggNOG" id="COG2138">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065901_2_0_0"/>
    <dbReference type="OMA" id="WREPFER"/>
    <dbReference type="OrthoDB" id="1573073at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="115" mass="13774" checksum="6391D3C8707CF4CF" modified="2009-02-10" version="1">MKGILLVAHGSRVQETKEVVYRYFEDLQRKYKNCKLGFMEFNEPSIEDAVKEFLKEKVEEIVVLPLFLFEGMHIKKDIPEILEKYPIKFKILKPILYDKKVSDILFERIEKSEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHL4</accession>
    <name>B7IHL4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydropteroate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">folP</name>
        <name evidence="2" type="ordered locus">THA_1125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101228.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1125"/>
    <dbReference type="EnsemblBacteria" id="ACJ75578">
        <property type="protein sequence ID" value="ACJ75578"/>
        <property type="gene ID" value="THA_1125"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1125"/>
    <dbReference type="eggNOG" id="COG0294">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008023_0_2_0"/>
    <dbReference type="OMA" id="FSIDTYH"/>
    <dbReference type="OrthoDB" id="1275854at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009396">
        <property type="term" value="P:folic acid-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="PTHR20941"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00793">
        <property type="entry name" value="DHPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pterin-binding" evidence="1">
        <location>
            <begin position="16"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="276" mass="31202" checksum="1F9C54EF66E77EB7" modified="2009-02-10" version="1">MKYSLKNGKEINFDHTYIMGIINVTPDSFYPGSRIDRDRLIDTVSQMIKDGAEIIDVGGESTRPGAQEVSLEEELNRVVPAIEIIKNNFDIIVSVDTYKSKVAEESLKLGADIINDISAFRFDENMVNIAKKYDCPVILMHMKGTPRNMQQNPFYEDTIGEIIKFLKERIEFSKKHGVEKIIIDPGIGFGKRLIDNLLILKHIDEFKKLGYPVLVGASRKSMIGMILDLPVEERLEGTLAITAYSVMKDVDIIRVHDVKENYRVVKVIEAIKNAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHM4</accession>
    <name>B7IHM4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580024.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1135"/>
    <dbReference type="EnsemblBacteria" id="ACJ75588">
        <property type="protein sequence ID" value="ACJ75588"/>
        <property type="gene ID" value="THA_1135"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1135"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062584_1_0_0"/>
    <dbReference type="OMA" id="VGVHEWK"/>
    <dbReference type="OrthoDB" id="785019at2"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003773">
        <property type="entry name" value="Menaquinone_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027024">
        <property type="entry name" value="UCP027386_ABC_sbc_TM0202"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02621">
        <property type="entry name" value="VitK2_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027386">
        <property type="entry name" value="UCP027386_ABC_sbc_TM0202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="302" mass="33777" checksum="8782E0C5D03742BF" modified="2009-02-10" version="1">MKKLVISIILLFSILQFGISLLNPFGPTIFPVAPILGEKVKGEVGISIELWKNLDEVVSKVSSNYSKFVVLPITTAANIYSSGVDIKLVGVHEWKVFYLIANEDFKDLSSLKGKTVYSAHGRGQTVDVLLRYLLVQNGLKPDVDVKFAYAAPQEIVALFKSKKINFAALPEPFVSMCLDSGKIVLDFQQEWNKISNSSLGIPIAGLFVVGKITEFENVINDVEKTFKKSVEYANSHIDEALEITSKYLPIPSAILKKSLERTTFKYIPINECKNEVHNFLKTMHELYPEGIPQVPDEGFYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHR0</accession>
    <name>B7IHR0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alanyl-tRNA synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1173</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580057.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1173"/>
    <dbReference type="EnsemblBacteria" id="ACJ75624">
        <property type="protein sequence ID" value="ACJ75624"/>
        <property type="gene ID" value="THA_1173"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1173"/>
    <dbReference type="eggNOG" id="COG2872">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_7_2_0"/>
    <dbReference type="OMA" id="IACQHTA"/>
    <dbReference type="OrthoDB" id="783542at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II_ALA" evidence="3">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="366" mass="41867" checksum="EA2EEE9A66E4B7FF" modified="2009-02-10" version="1">MKIRIEEVLKEKGKIFAISSESPFYVDYKGGQLGDRGKIGNASVLSVMSKNGKIYHEIDREVDLGENDVEIDLNHQLFVRQHHTGQHILSAVFEELADISTVGFRMGFEYTTIDLNVPYIVEELLDEVEERVNSIITECVDVQEILVEKEEVDRFPLRKKLSDKVEGMVRIIKIGEYDYSPCGGYHVKNTGQIGLLKILKTEKVKGELTRVYFVAGFKAMEYFRKYSKILKNISVSLTSSIFEVEDKVRSLLSEVKEKSSRLENLAEKLACYKKDDLKKLKEDVYFLQEDPEILRYIPKYFDKEGLLVLYDGKSYSFTSNSSKYIVRNIISDLRERFGGKGGGGKEKGNYLPSDGVNINMILEVLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHW1</accession>
    <name>RL6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplF</name>
        <name type="ordered locus">THA_1230</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101466.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHW1"/>
    <dbReference type="SMR" id="B7IHW1"/>
    <dbReference type="STRING" id="484019.THA_1230"/>
    <dbReference type="EnsemblBacteria" id="ACJ75675">
        <property type="protein sequence ID" value="ACJ75675"/>
        <property type="gene ID" value="THA_1230"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1230"/>
    <dbReference type="eggNOG" id="COG0097">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065464_1_2_0"/>
    <dbReference type="OMA" id="KPDPYKG"/>
    <dbReference type="OrthoDB" id="1398618at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_B">
        <property type="entry name" value="Ribosomal_L6_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_L6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_L6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_L6_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019906">
        <property type="entry name" value="Ribosomal_L6_bac-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="PTHR11655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF14">
        <property type="entry name" value="PTHR11655:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00059">
        <property type="entry name" value="RIBOSOMALL6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="SSF56053"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03654">
        <property type="entry name" value="L6_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000144060" description="50S ribosomal protein L6">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01365"/>
        </source>
    </evidence>
    <sequence length="184" mass="20770" checksum="ABDDA026D8BCC6DF" modified="2009-02-10" version="1">MSRIANKPIVIPNGVEVKVEGNVLKVKGPLGELKQDFLPYVKVEINGNEMNVKPNVDYMKRRSDLKKMKMFTGTYWRLFNNMVIGVTKGFKKELEIVGIGYRAQLQGKKLVMNLGYAHPVEMEIPSDVKVEVPSPNKIIVSGIDKQRVGQVAADIRKWREPNVYSGKGIRYVGEVVRLKEGKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHW6</accession>
    <name>B7IHW6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Protein translocase subunit SecY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secY</name>
        <name evidence="13" type="ordered locus">THA_1235</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 12">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580082.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1235"/>
    <dbReference type="EnsemblBacteria" id="ACJ75680">
        <property type="protein sequence ID" value="ACJ75680"/>
        <property type="gene ID" value="THA_1235"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1235"/>
    <dbReference type="eggNOG" id="COG0201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030313_0_2_0"/>
    <dbReference type="OMA" id="FAMWLGE"/>
    <dbReference type="OrthoDB" id="1567535at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="PTHR10906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="SSF103491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 11" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ75680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="428" mass="47240" checksum="9AE18DC2989BC076" modified="2009-02-10" version="1">MWKALKNAFRIPELRDRIVFTLLMLIVFRLGIYVPVPGVNLKAWGAAFSQMGTGSAGGLLSFYDVFTGGAFRSFSIFALSVTPYINASIILQLLSSVIPSLKEMLKEGEEGRKKFQKLTRNLTVLLGALQAFVISFGLARGFENILVIPLWTFVFLATVSLLAGTMFLLWIGDRITEKGIGNGISVLIFAGIVARYPTYFRTAVLGGLNIFEWIFLLGVMFLMVIGIIYVQQAERRIMVQYASRMVGRRIYGGTSTHIPIKVNHSGVIPIIFGWAIVSIPVGIAQLTNSETAKSLFSMTSPLVITIYAVLIFFFTYFYSVVVFDPKDISQNIKSYGGYIPGIRPGKPTEQYITRVLNRITFVGALFLVAIALVPYLVQGVTGVNIWLGGTSALIAVGVALDIAQQMEAHLIMRNYEGFVKKGKLPGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHY1</accession>
    <name>B7IHY1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rluD</name>
        <name evidence="5" type="ordered locus">THA_1250</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1250"/>
    <dbReference type="EnsemblBacteria" id="ACJ75695">
        <property type="protein sequence ID" value="ACJ75695"/>
        <property type="gene ID" value="THA_1250"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1250"/>
    <dbReference type="eggNOG" id="COG0564">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016902_11_2_0"/>
    <dbReference type="OMA" id="AHPSPGW"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140098">
        <property type="term" value="F:catalytic activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034470">
        <property type="term" value="P:ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PseudoU_synth_2" evidence="4">
        <location>
            <begin position="27"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="245" mass="27802" checksum="50421E1A785F42DD" modified="2009-02-10" version="1">MEFPEKPKEVEILPENIPLDILYEDKDIVVVNKSAGMITHPTPSFTSGTLVNALMYHCKDFQGISGELRPGIVHRLDKDTSGVIVVAKNDKAHQSLSKQFKDRVTEKVYAAIVKGIVKKDYGTIEVPVGRNPVVRTKMAAVDWGKNALTYFKVLRRFKDATLVFAYPKTGRTHQIRIHMKYIGHPLLGDELYGSGKKDEIYGATRQMLHALRLSFYHPTTEEKMTFVAPLPEDFKRVLKILSEIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMW5</accession>
    <name>C0QMW5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase repeat family ISChy1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_2039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580554.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2039"/>
    <dbReference type="EnsemblBacteria" id="ACN29532">
        <property type="protein sequence ID" value="ACN29532"/>
        <property type="gene ID" value="THA_2039"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2039"/>
    <dbReference type="eggNOG" id="COG3547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036902_4_4_0"/>
    <dbReference type="OMA" id="TRMRYHL"/>
    <dbReference type="OrthoDB" id="655500at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transposase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEDD_Tnp_IS110" evidence="1">
        <location>
            <begin position="20"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACN29532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="382" mass="43935" checksum="4E8C36BB0B2D65C6" modified="2009-05-05" version="1">MAILAIDVSKSSLSFYSDFIGKGFVDNSPQGIFDLFNKASDSSSDFILVLESTGIYSFDVANYFFQNHIPVFWVKTDNMKLYRKILNRPKTDKIDAKLIFDIASKFPDSLVPFVNNSYIISELRNLTRLYLKLNEDIARLKLRLYSYVSLYFPKLDFKLNKTYESLLKDFTVEEIATMPLEELFEYIAKVSRHIVSSKDLAEKIQSLAKNALRLSPNPSNTLRLSIVSTIELIQHYEKQINLVKKEISKLIKRIPNTLTTVKGIGDITAAGIIAEIGDINRFRNASALASYAGLVWNIKQSGNYKSENTQLTKKGNKYLRTYLVMAANSLRIYDPIFKDYYQRKYTQTTTHKHMRALILSARKLVNLVYYLLKNNVPYVPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICJ6</accession>
    <name>B7ICJ6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Integrase, catalytic region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1010</name>
        <name evidence="7" type="ordered locus">THA_1107</name>
        <name evidence="8" type="ordered locus">THA_1267</name>
        <name evidence="9" type="ordered locus">THA_1278</name>
        <name evidence="10" type="ordered locus">THA_1436</name>
        <name evidence="11" type="ordered locus">THA_1983</name>
        <name evidence="2" type="ordered locus">THA_503</name>
        <name evidence="3" type="ordered locus">THA_768</name>
        <name evidence="4" type="ordered locus">THA_818</name>
        <name evidence="5" type="ordered locus">THA_985</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 12">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1010"/>
    <dbReference type="EnsemblBacteria" id="ACJ74992">
        <property type="protein sequence ID" value="ACJ74992"/>
        <property type="gene ID" value="THA_503"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75232">
        <property type="protein sequence ID" value="ACJ75232"/>
        <property type="gene ID" value="THA_768"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75280">
        <property type="protein sequence ID" value="ACJ75280"/>
        <property type="gene ID" value="THA_818"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75444">
        <property type="protein sequence ID" value="ACJ75444"/>
        <property type="gene ID" value="THA_985"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75468">
        <property type="protein sequence ID" value="ACJ75468"/>
        <property type="gene ID" value="THA_1010"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75560">
        <property type="protein sequence ID" value="ACJ75560"/>
        <property type="gene ID" value="THA_1107"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75712">
        <property type="protein sequence ID" value="ACJ75712"/>
        <property type="gene ID" value="THA_1267"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75723">
        <property type="protein sequence ID" value="ACJ75723"/>
        <property type="gene ID" value="THA_1278"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75880">
        <property type="protein sequence ID" value="ACJ75880"/>
        <property type="gene ID" value="THA_1436"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76404">
        <property type="protein sequence ID" value="ACJ76404"/>
        <property type="gene ID" value="THA_1983"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1010"/>
    <dbReference type="KEGG" id="taf:THA_1107"/>
    <dbReference type="KEGG" id="taf:THA_1267"/>
    <dbReference type="KEGG" id="taf:THA_1278"/>
    <dbReference type="KEGG" id="taf:THA_1436"/>
    <dbReference type="KEGG" id="taf:THA_1983"/>
    <dbReference type="KEGG" id="taf:THA_503"/>
    <dbReference type="KEGG" id="taf:THA_768"/>
    <dbReference type="KEGG" id="taf:THA_818"/>
    <dbReference type="KEGG" id="taf:THA_985"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052819_0_0_0"/>
    <dbReference type="OMA" id="SHFKAEC"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025948">
        <property type="entry name" value="HTH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13276">
        <property type="entry name" value="HTH_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="100"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ76404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="273" mass="32794" checksum="41F7823941FD6A95" modified="2009-02-10" version="1">MIEEGFSVTEAVRYLKISRSTYYYKPKKYKRKKNDESILKRIQEIKQEHPYWGYRRICAMLRKQGEKINHKRVYRIMKENRLLLKQYKMKAKRSFQRKIIPNKPRQVLGIDMTKVMTKDGGWVNYIAVIDWYTREVLGSQISLRGRTEEWLKALNNALNKGYKDGVRGREVILVSDNGTQPTSRRFMEECKILGIKQIFTSYNNPKGNANTERYFRTYKEEVVWPVEEMSYSELVARTKEYEEFYNSKYPHSALKYRSPREVYEEYLSFTKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICL0</accession>
    <name>B7ICL0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Unknwon conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1292</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 130 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580125.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1292"/>
    <dbReference type="EnsemblBacteria" id="ACJ75737">
        <property type="protein sequence ID" value="ACJ75737"/>
        <property type="gene ID" value="THA_1292"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1292"/>
    <dbReference type="eggNOG" id="COG2152">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046648_0_0_0"/>
    <dbReference type="OMA" id="EKFYQIE"/>
    <dbReference type="OrthoDB" id="1622507at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007184">
        <property type="entry name" value="Mannoside_phosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34106">
        <property type="entry name" value="PTHR34106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04041">
        <property type="entry name" value="Glyco_hydro_130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016202">
        <property type="entry name" value="PH1107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="312" mass="36226" checksum="D75F8F627FFC131D" modified="2009-02-10" version="1">MIPWEDRPKDCSDVIWRYSNNPVIQRNQARNSNSIFNSAVVNFKEGFAGVFRVDDTERNMNLRRGFSKDGINWKIDDNTIDFIRENEEIESEYKYDPRVVYIEDRYWITWCNGYHGPTIGVGFTFDFKKFYQIENAFLPYNRNGVLFPRKINGKYAMLSRPSDKGHTPFGDIFYSESPDMIHWGNHRFVMGSGYSPWQSLKIGAGPSPIETKEGWLLFYHGVLLSCNGYVYSFGAALLDLDKPWKVIKRSKKYLLSPQMIYEQIGDVQNVVFPVSALVEDNKVAIYYGAADTVTALCFGYINEIIDWLKYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICM0</accession>
    <name>B7ICM0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1302</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580133.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1302"/>
    <dbReference type="EnsemblBacteria" id="ACJ75747">
        <property type="protein sequence ID" value="ACJ75747"/>
        <property type="gene ID" value="THA_1302"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1302"/>
    <dbReference type="eggNOG" id="ENOG5033AS9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2620877_0_0_0"/>
    <dbReference type="OMA" id="GPCSNIN"/>
    <dbReference type="OrthoDB" id="2055291at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035205">
        <property type="entry name" value="DUF5320"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17253">
        <property type="entry name" value="DUF5320"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="78" mass="8629" checksum="35FA220832A620F1" modified="2009-02-10" version="1">MPGFDGTGPMGTGPVGRRLGPCSNINAPYTPRYGWFKPVVGWFYGYGRGFGRGYGRFAGYDRGRFGRGAGRGFGRFYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICP0</accession>
    <name>B7ICP0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Modification methylase, HemK family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101654.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1322"/>
    <dbReference type="EnsemblBacteria" id="ACJ75767">
        <property type="protein sequence ID" value="ACJ75767"/>
        <property type="gene ID" value="THA_1322"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1322"/>
    <dbReference type="eggNOG" id="COG3872">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038140_0_0_0"/>
    <dbReference type="OMA" id="LFQYHGA"/>
    <dbReference type="OrthoDB" id="1816476at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010787">
        <property type="entry name" value="DUF1385"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07136">
        <property type="entry name" value="DUF1385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="286" mass="32528" checksum="629968B9DE8B14EF" modified="2009-02-10" version="1">MKVGGQAIIEGVLMMGKKIVIAVRNEKGEIVKEELGKIKNKKVLKIPFVRGLFSLFYSMYFGLKGLDRSAEIATGEEMKKSETFFSIFIAIVVGIGLFVMLPVFLTGLLGVKDDEFLFSLVDGFIRLGLFLLYVWIISFMKDVKRVFQYHGSEHKTIHAYENGDELTVENVKKYSTIHPRCGTNFVMIFLIVAIILFSIFGIYRPLSWVERIVIRIVLIPVVASVSYELLKLFDKFPFLRFLSFPGLLLQKLTTAEPDEKQIEVAIESLKFALEGEEPDEDVEYVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICP8</accession>
    <name>B7ICP8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1330"/>
    <dbReference type="EnsemblBacteria" id="ACJ75775">
        <property type="protein sequence ID" value="ACJ75775"/>
        <property type="gene ID" value="THA_1330"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1330"/>
    <dbReference type="eggNOG" id="COG2052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193807_0_0_0"/>
    <dbReference type="OMA" id="ECKSIVF"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007169">
        <property type="entry name" value="RemA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04025">
        <property type="entry name" value="DUF370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="78" mass="8795" checksum="D59996C8EECD45C6" modified="2009-02-10" version="1">MEQIVKISNNLYIPRNRIIAVLPISSAVARKIRGMHKIGGNIINMTFGEESKSIIIMDSGHIFVTSKTLEEVDEALWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICV8</accession>
    <name>B7ICV8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580200.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1391"/>
    <dbReference type="EnsemblBacteria" id="ACJ75835">
        <property type="protein sequence ID" value="ACJ75835"/>
        <property type="gene ID" value="THA_1391"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1391"/>
    <dbReference type="eggNOG" id="ENOG502ZQK6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_204730_0_0_0"/>
    <dbReference type="OMA" id="ISLRYMR"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="66" mass="7471" checksum="CF19D0E996841247" modified="2009-02-10" version="1">MKLLSNLFFVGATIVFLISIIFFEIGLRAMRRENEKKTKESNKLGIRFLILSGILFGLSGLTAFFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICW2</accession>
    <name>B7ICW2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dipeptide ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1395</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580203.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1395"/>
    <dbReference type="EnsemblBacteria" id="ACJ75839">
        <property type="protein sequence ID" value="ACJ75839"/>
        <property type="gene ID" value="THA_1395"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1395"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_341533_0_0_0"/>
    <dbReference type="OMA" id="IPNVMPL"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="637"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="672"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="697"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="756"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="807"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="633"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="842" mass="96109" checksum="A070EB21DA9A4D83" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICX7</accession>
    <name>YBEY_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endoribonuclease YbeY</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ybeY</name>
        <name type="ordered locus">THA_1410</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the endoribonuclease YbeY family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101813.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ICX7"/>
    <dbReference type="SMR" id="B7ICX7"/>
    <dbReference type="STRING" id="484019.THA_1410"/>
    <dbReference type="EnsemblBacteria" id="ACJ75854">
        <property type="protein sequence ID" value="ACJ75854"/>
        <property type="gene ID" value="THA_1410"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1410"/>
    <dbReference type="eggNOG" id="COG0319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106710_3_3_0"/>
    <dbReference type="OMA" id="INYIFCD"/>
    <dbReference type="OrthoDB" id="1830156at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00009">
        <property type="entry name" value="Endoribonucl_YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023091">
        <property type="entry name" value="MetalPrtase_cat_dom_sf_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002036">
        <property type="entry name" value="YbeY"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02130">
        <property type="entry name" value="YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00043">
        <property type="entry name" value="TIGR00043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000200003" description="Endoribonuclease YbeY">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00009"/>
        </source>
    </evidence>
    <sequence length="134" mass="15764" checksum="D3FF335ADDABB2DF" modified="2009-02-10" version="1">MVTTNKQIEEGYLKYMDEILKTEIGKDVNVNLVFIDKGEIERLNNQYRNINAPTDVLTFVYGDDDLYAEIFVCEDVIKENAEKFYNTFEKELLTVLIHAALHVAGYDHEYDTTNAKEMFQKQEEYLKKYDVDNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICZ4</accession>
    <name>B7ICZ4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine/threonine protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580221.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1427"/>
    <dbReference type="EnsemblBacteria" id="ACJ75871">
        <property type="protein sequence ID" value="ACJ75871"/>
        <property type="gene ID" value="THA_1427"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1427"/>
    <dbReference type="eggNOG" id="COG0639">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023125_4_0_0"/>
    <dbReference type="OMA" id="MISINID"/>
    <dbReference type="OrthoDB" id="900869at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="4"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="253" mass="29300" checksum="B57451B310CDF078" modified="2009-02-10" version="1">MISVFSDIHGCFYEFHLLLDYINKKFGKFKIRYIFLGDYIDRGPSSKEVVDKIIEIGPKVCLLGNHEDMMIRYIKTGRSSWLSENNGGIYTLKSFSSIEKIKDYFFFFENLKFLHVETLFGKVRFLFSHANLPSNYPLDEAIRWKGDYSSFLMENEIPLSESNIWYRIAEPIEGYVLVHGHTPTYDGKISFLKINDKIVDINIDTGCVFGGHLTTLLIPENEQELKETSYNLIAINEKIDEEKISIFELGGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICZ9</accession>
    <name>B7ICZ9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein, Hsp20 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1432</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101856.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="SMR" id="B7ICZ9"/>
    <dbReference type="STRING" id="484019.THA_1432"/>
    <dbReference type="EnsemblBacteria" id="ACJ75876">
        <property type="protein sequence ID" value="ACJ75876"/>
        <property type="gene ID" value="THA_1432"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1432"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_12_3_0"/>
    <dbReference type="OMA" id="DESAQWL"/>
    <dbReference type="OrthoDB" id="1523369at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="30"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="4">
        <location>
            <begin position="34"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="142" mass="16848" checksum="7268FE84B9B47EE8" modified="2009-02-10" version="1">MLARRNYFDPFVELQREIDRLFEDFVRPSRFDTTHFPKVDVYETDKEVVIEAELPGLKKDDVKITIEDNVLTIKGERKFNREDKGKNYKIIERAEGYFERSFGLPEYVDVEKIKAKFNDGVLTIELPKKETKDKKVIDIQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID79</accession>
    <name>B7ID79_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Coiled-coil domain containing 70</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102043.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1515"/>
    <dbReference type="EnsemblBacteria" id="ACJ75956">
        <property type="protein sequence ID" value="ACJ75956"/>
        <property type="gene ID" value="THA_1515"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1515"/>
    <dbReference type="HOGENOM" id="CLU_2572730_0_0_0"/>
    <dbReference type="OMA" id="WNELNQA"/>
    <dbReference type="OrthoDB" id="2077891at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="81" mass="9654" checksum="2A3FFF683E6D5A94" modified="2009-02-10" version="1">MEEKIKELEQAFEDLINKYQELQKENKELWKELNAAIERANQLEQENRTLKEEINGTFDKLLGKLKNLMESSTEINDQEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDA5</accession>
    <name>B7IDA5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1542</name>
        <name evidence="2" type="ordered locus">THA_1595</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75982">
        <property type="protein sequence ID" value="ACJ75982"/>
        <property type="gene ID" value="THA_1542"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76033">
        <property type="protein sequence ID" value="ACJ76033"/>
        <property type="gene ID" value="THA_1595"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1542"/>
    <dbReference type="KEGG" id="taf:THA_1595"/>
    <dbReference type="HOGENOM" id="CLU_3048930_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="54" mass="6123" checksum="65D4230B776CAD31" modified="2009-02-10" version="1">MKKVSPPDNIELTTNKNQEAIRMKQKSINKKISRISQDTLIVGIDLQKELIGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDB0</accession>
    <name>B7IDB0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1548</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102216.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1548"/>
    <dbReference type="EnsemblBacteria" id="ACJ75987">
        <property type="protein sequence ID" value="ACJ75987"/>
        <property type="gene ID" value="THA_1548"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1548"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_0"/>
    <dbReference type="OMA" id="PRNMYDK"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03301">
        <property type="entry name" value="ABC_MalK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015855">
        <property type="entry name" value="ABC_transpr_MalK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040582">
        <property type="entry name" value="OB_MalK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005116">
        <property type="entry name" value="Transp-assoc_OB_typ1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17912">
        <property type="entry name" value="OB_MalK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03459">
        <property type="entry name" value="TOBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="367" mass="41516" checksum="D9D5EB0D26DAC452" modified="2009-02-10" version="1">MAKVELEHVWKIYDGKVEAVKDATFTIEDKEFVVLLGPSGCGKTTTLRMIAGLEEITKGTLKIDGKVMNDVEPKDRDIAMVFQNYALYPHMTVYDNMAFGLKLRKVPKDEIDRRVKEAAKILGIEEYLDRKPRQLSGGQRQRVAVGRAIVRNPKVFLFDEPLSNLDAKLRTQMRSELKKLHHRLEATIVYVTHDQIEAMTMADKIIVMKDGVIQQIGSPHEIYNKPANTFVAGFIGSPAMNFVNAKIIRENGLWIKSSGLKLKVPSQFEETLEKYIDKDIIFGIRPENIYDKMFAMLPKEGENTARAKVDVVEPLGSETILHLVAGEDKLVAVVDPKTEAKEEQEIDLVFDMNTMHVFDKETGKAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDC0</accession>
    <name>B7IDC0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Fe-hydrogenase gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1559</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="6">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580275.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1559"/>
    <dbReference type="EnsemblBacteria" id="ACJ75997">
        <property type="protein sequence ID" value="ACJ75997"/>
        <property type="gene ID" value="THA_1559"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1559"/>
    <dbReference type="eggNOG" id="COG1905">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054362_2_1_0"/>
    <dbReference type="OMA" id="HIIRYCD"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028431">
        <property type="entry name" value="NADP_DH_HndA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43342:SF2">
        <property type="entry name" value="PTHR43342:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="163" mass="18143" checksum="F8E5D77BC206B2C7" modified="2009-02-10" version="1">MLAVCEKHVELYKELDAYIEELKGKKGILINVLHKAQELFGWLPQEVQDHVAKKLNIPSSVVYGVVTFYNFFSTKPKGKNQIKICLGTACYVKGADRVMERFLNELGVEESEVTKDGKFSVHGVRCLGACSMAPVVLVGEKDFYGKVTPDMVPGIIKKYKGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDC2</accession>
    <name>B7IDC2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="5">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1561</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102244.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1561"/>
    <dbReference type="EnsemblBacteria" id="ACJ75999">
        <property type="protein sequence ID" value="ACJ75999"/>
        <property type="gene ID" value="THA_1561"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1561"/>
    <dbReference type="eggNOG" id="COG0483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_0_0_0"/>
    <dbReference type="OMA" id="VHQELEF"/>
    <dbReference type="OrthoDB" id="1741160at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052832">
        <property type="term" value="F:inositol monophosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052833">
        <property type="term" value="F:inositol monophosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="255" mass="28805" checksum="ED2B382D6B0BAA99" modified="2009-02-10" version="1">MNKLDLAINIAKSAGYYILQYWGNVDNVYEKENFQDLVTDYDKNAQNMIVSKIKKFFPEDGFIAEEGNLYEKSKNLWIIDPIDGTINYIHGLPNFGVSIAYFENEKPIFGVVFNPFTEELFVGIKDEGSFLNHSRLKITKNITLKESIGSTGFHKNFTGKFISNVENKVQRIRIIGSAALSACYVAANKFDFFVAKRANSWDIAAAYIIVKEAGGKIINFNGNTPKLFSKDSYIFSNPSISDEILELIKKQEGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDC9</accession>
    <name>B7IDC9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribose 5-phosphate isomerase B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpiB</name>
        <name evidence="4" type="ordered locus">THA_1568</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LacAB/RpiB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102294.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1568"/>
    <dbReference type="EnsemblBacteria" id="ACJ76006">
        <property type="protein sequence ID" value="ACJ76006"/>
        <property type="gene ID" value="THA_1568"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1568"/>
    <dbReference type="eggNOG" id="COG0698">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091396_4_1_0"/>
    <dbReference type="OMA" id="DYPPFCL"/>
    <dbReference type="OrthoDB" id="1346802at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016861">
        <property type="term" value="F:intramolecular oxidoreductase activity, interconverting aldoses and ketoses"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004785">
        <property type="entry name" value="RpiB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003500">
        <property type="entry name" value="RpiB_LacA_LacB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036569">
        <property type="entry name" value="RpiB_LacA_LacB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30345">
        <property type="entry name" value="PTHR30345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02502">
        <property type="entry name" value="LacAB_rpiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005384">
        <property type="entry name" value="RpiB_LacA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89623">
        <property type="entry name" value="SSF89623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01120">
        <property type="entry name" value="rpiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00689">
        <property type="entry name" value="rpiB_lacA_lacB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="3">
        <location>
            <begin position="8"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="3">
        <location>
            <begin position="66"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005384-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005384-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="145" mass="15913" checksum="21775830CCED305F" modified="2009-02-10" version="1">MKIAIGSDHAGFELKNKLVDYLSKKGIEIIDVGTNSTESVDYPDYAKEVGKLVVNKDADFGILICGTGIGMSIAANKIKGIRAALCMIPEMGKLARNHNNANILVLPGRLIGFELATWIVEEFLNADFEGGRHERRINKIVELEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDF8</accession>
    <name>B7IDF8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1597</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580285.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1597"/>
    <dbReference type="EnsemblBacteria" id="ACJ76035">
        <property type="protein sequence ID" value="ACJ76035"/>
        <property type="gene ID" value="THA_1597"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1597"/>
    <dbReference type="HOGENOM" id="CLU_1685731_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="156" mass="18135" checksum="1D7D2A5B7A8C3C68" modified="2009-02-10" version="1">MKIQILLLIFVSLLSFSNNHIIGKVNIKVPPEINTNIKTFEYNLCNTYEENNCQNSLLLKMNYSSNYLVFLVISYPKTIIDNFIFVSVKSYINNFPTANFWYTGSKCYSSDNYVLTPLSNNGIVKFFFKNSKKSKTIPAGKYTIPIEFKFYPIVKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDG0</accession>
    <name>B7IDG0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580287.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1599"/>
    <dbReference type="EnsemblBacteria" id="ACJ76037">
        <property type="protein sequence ID" value="ACJ76037"/>
        <property type="gene ID" value="THA_1599"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1599"/>
    <dbReference type="eggNOG" id="COG3629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_976388_0_0_0"/>
    <dbReference type="OMA" id="CRNDITV"/>
    <dbReference type="OrthoDB" id="789803at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="285" mass="33745" checksum="C4727C62FA861E20" modified="2009-02-10" version="1">MKEGESIKVITFGDFLISELKNTHFSPKESEILAFVIYKKHVSVENVLENVWDINQMPCRNDITVFFSKINKKLKNIARLNTKDSIVSVVPNKIETDFENFERLSLVSLDKKDTKSLKKAFDIYNGDFLPSISSDWALSVRMYYREAFFEVGKYLVYSETNVTNEMMYLKKMIDLGLDYNVVKLIVEEIEKRYEFDLVYEDFFDYVLLKNKLMRFSSYIGMVIIFDKEFPLEDNIRKGDIILKIDTLTYKILIERNWKLSKEKDIELFLEKLKNKNAKIKKVEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDH4</accession>
    <name>B7IDH4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fba fructose-1,6-bisphosphate aldolase, class II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1613</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102382.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1613"/>
    <dbReference type="EnsemblBacteria" id="ACJ76051">
        <property type="protein sequence ID" value="ACJ76051"/>
        <property type="gene ID" value="THA_1613"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1613"/>
    <dbReference type="eggNOG" id="COG0191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040088_0_0_0"/>
    <dbReference type="OMA" id="PRTWGKL"/>
    <dbReference type="OrthoDB" id="827430at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00947">
        <property type="entry name" value="TBP_aldolase_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000771">
        <property type="entry name" value="FBA_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011289">
        <property type="entry name" value="Fruc_bis_ald_class-2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01116">
        <property type="entry name" value="F_bP_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001359">
        <property type="entry name" value="F_bP_aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00167">
        <property type="entry name" value="cbbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01859">
        <property type="entry name" value="fruc_bis_ald"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00602">
        <property type="entry name" value="ALDOLASE_CLASS_II_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Dihydroxyacetone phosphate binding" evidence="2">
        <location>
            <begin position="215"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Dihydroxyacetone phosphate binding" evidence="2">
        <location>
            <begin position="257"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="312" mass="33996" checksum="0CBF464D1344F728" modified="2009-02-10" version="1">MYVNTKDILEKASKEFYAVPAFNINNLEFLIAILEGAVEKNAPVIIETSEGAIKYAGNGDPIRGAKFFVNLVKGYADTLNIPVALHLDHGKNIEYIAAAIKAGYSSVMIDASHEPFEENLKITKEVVKWAHAAGVSVEAELGQLAGIEDNVSAEENVLVDPEQAKIFVEETGVDFLAPAIGTSHGAFKFKGEAKLDFERLEKVKKLTNIPLVLHGASSVPVKYVELAEKYGADLGGAKGVPAEEIKKCVKLGINKVNTDTDLRIAFLAGLRKNLTENVKEFDPRKYFKEGMQFVKDVVMDRLEFLGAAGKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDH7</accession>
    <name>B7IDH7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 8" type="primary">ftsZ</name>
        <name evidence="8" type="ordered locus">THA_1616</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102388.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1616"/>
    <dbReference type="EnsemblBacteria" id="ACJ76054">
        <property type="protein sequence ID" value="ACJ76054"/>
        <property type="gene ID" value="THA_1616"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1616"/>
    <dbReference type="eggNOG" id="COG0206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024865_0_1_0"/>
    <dbReference type="OMA" id="GNPSIGQ"/>
    <dbReference type="OrthoDB" id="1009041at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="PTHR30314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="SSF52490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="SSF55307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 5 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin" evidence="6">
        <location>
            <begin position="17"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin_C" evidence="7">
        <location>
            <begin position="211"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="25"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="112"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="351" mass="37872" checksum="52643C4234A4B8E4" modified="2009-02-10" version="1">MGFSVKREEVFSKIMPKIKVVGVGGAGCNAINRMIEFGIDDVSFVAVNTDAQVLEVSKADEIVQIGEKLTKGLGAGGNPKVGEEAALEDKKKLEEMLRGIDMLFIAAGFGGGTGTGAAPVIAEIAKSLGILTVAVVTTPFYFEGAPRWRAAMEGVKKIHKNVDTLIKISNNKLLEELSWDIPFVEAFAKADETLYQGIKGISELITKRGIINLDFADIESVMRNAGAAMLGIGVAKGENRATVAARRALESKLVEHPIENATKLIMNITASTTFKLHEMQEAATIIRQTCSEDADLKLGIIVDPEIPEDELRVTLIATGLEREEDFLYSDDDIPAIFKFGLEVMGDDGEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDJ5</accession>
    <name>B7IDJ5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Flagellar hook protein FlgE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1634</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580313.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1634"/>
    <dbReference type="EnsemblBacteria" id="ACJ76072">
        <property type="protein sequence ID" value="ACJ76072"/>
        <property type="gene ID" value="THA_1634"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1634"/>
    <dbReference type="eggNOG" id="COG1749">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013687_2_1_0"/>
    <dbReference type="OMA" id="GLQNHQT"/>
    <dbReference type="OrthoDB" id="704242at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.98.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011491">
        <property type="entry name" value="Falgellar_hook_FlgE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037058">
        <property type="entry name" value="Falgellar_hook_FlgE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019776">
        <property type="entry name" value="Flagellar_basal_body_rod_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020013">
        <property type="entry name" value="Flagellar_FlgE/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037925">
        <property type="entry name" value="FlgE/F/G-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07559">
        <property type="entry name" value="FlaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117143">
        <property type="entry name" value="SSF117143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03506">
        <property type="entry name" value="FlgEFG_subfam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00588">
        <property type="entry name" value="FLAGELLA_BB_ROD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="4">
        <location>
            <begin position="5"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="FlaE" evidence="6">
        <location>
            <begin position="446"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="5">
        <location>
            <begin position="625"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="704" mass="75058" checksum="66D0688D3267BE58" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDJ8</accession>
    <name>B7IDJ8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Motility protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1637</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MotB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102432.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1637"/>
    <dbReference type="EnsemblBacteria" id="ACJ76075">
        <property type="protein sequence ID" value="ACJ76075"/>
        <property type="gene ID" value="THA_1637"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1637"/>
    <dbReference type="eggNOG" id="COG1360">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_958927_0_0_0"/>
    <dbReference type="OMA" id="NWHLSSA"/>
    <dbReference type="OrthoDB" id="1619550at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025713">
        <property type="entry name" value="MotB_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13677">
        <property type="entry name" value="MotB_plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="OmpA-like" evidence="5">
        <location>
            <begin position="101"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="288" mass="32807" checksum="E1A50D9B7728E47A" modified="2009-02-10" version="1">MAKKQECPKGAPLWMTTYGDMVTLLLTFFVLLFAMSSISPGKFQQVAVGLTSALNGNPPSVLTGGKSLQTEPLISQNPGVYQEILKISEEYKGKVTVEERDEGTLITISDLDIFNPGSARLTAEAKKLLEKLGAVIIEHTTNVLEIYGYANEIPVPENSIYQSNWHLSSARASSVVYFFTTELKQKRTLERIADIQLGRFDPDYYYAAERFLPIGKGDIEIQKEIKALRNLYEFELEEMRKKLVAGEITREEWNNFVAQKTEETDNKIRQLREKYRRIDILIKRELAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDK2</accession>
    <name>B7IDK2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase family associated with various cellular activities (AAA) protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1641</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1641"/>
    <dbReference type="EnsemblBacteria" id="ACJ76079">
        <property type="protein sequence ID" value="ACJ76079"/>
        <property type="gene ID" value="THA_1641"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1641"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053995_0_0_0"/>
    <dbReference type="OMA" id="YQVVDMD"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="3"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="328" mass="37697" checksum="C6569C1259F0A6EA" modified="2009-02-10" version="1">MESGEIPLLVGHFGVGKTDIIREIARETNRELIILVLSQMEPGDLIGLPSRQDDKTVFLEPDWWPKNNQTILFLDEINRSHRSIRNAIMQLLVDKRIHNHILPEGTWIVASMNPPDEEYDQVDLITDPAFLSRFFILEITPDLEEWLKWAQKNDIPNEIIKFIEKNPEFLYSSNHISLKTTIKPSPRSWHKLGNVFKKLSDEEKKIFGYQLASGILGPEAAKIFTENLFLNIPTANQLLLEGIIPEKLELHEANSIILRIIDFFSNLDEENAKKLHEKAEIIAGNLIKFSNSVPKDSFFSLVRLLSELSNENGYKGKLCDKILEKISY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDL4</accession>
    <name>B7IDL4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine phosphatase RsbU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1653</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102458.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1653"/>
    <dbReference type="EnsemblBacteria" id="ACJ76091">
        <property type="protein sequence ID" value="ACJ76091"/>
        <property type="gene ID" value="THA_1653"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1653"/>
    <dbReference type="eggNOG" id="COG2172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090336_24_1_0"/>
    <dbReference type="OMA" id="DFGPKVD"/>
    <dbReference type="OrthoDB" id="1832160at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HATPase_c" evidence="1">
        <location>
            <begin position="10"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="135" mass="15589" checksum="AE88B2D111934F47" modified="2009-02-10" version="1">MDIISITFLSKSENIKIARSVVQKFLSQKAVNEQDIFDTELAVNEAIANIIEHTYKFEKDKIIKMTLKWTEPNVLEIYLRDFGPKVDPSKVKPRPLEEIRPGGLGVYIIQKIFDVMEFKDVSCGNLLLLKKEYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDP1</accession>
    <name>B7IDP1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lipopolysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1681</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
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        <property type="protein sequence ID" value="ACJ76118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580344.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1681"/>
    <dbReference type="EnsemblBacteria" id="ACJ76118">
        <property type="protein sequence ID" value="ACJ76118"/>
        <property type="gene ID" value="THA_1681"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1681"/>
    <dbReference type="eggNOG" id="COG0677">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_3_2_0"/>
    <dbReference type="OMA" id="GHCLPID"/>
    <dbReference type="OrthoDB" id="295459at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028359">
        <property type="entry name" value="UDP_ManNAc/GlcNAc_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500136">
        <property type="entry name" value="UDP_ManNAc_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDPG_MGDP_dh_C" evidence="4">
        <location>
            <begin position="329"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="435" mass="49269" checksum="A1789DE232FD29E4" modified="2009-02-10" version="1">MSKLYEKILNKSAIIGVMGMGYVGLPLAVEKARAGYNVIGFDIQQKRVDMINKGKNYIGDVNDEELKQLVSEKRLRATTNFDELENCDVISICVPTPLDKFKQPDLSYIIQTAEDISKRLRREQLIILESTTYPGTTEEVVLPILLKSGLEVGKDFYLAFSPERVDPGNIRYKTRNTPKVVGGVTSECTKYAKALYENVLDAPVFPVSSPKTAEMSKILENTFRLVNIALVQEMTKVAEKMNVNIWEVIDAASTKPFGYMPFYPGPGIGGHCIPIDPFYLSYKAREYDLHLMLVEQAGQVADEMPYYVVQRLGDILNEFKKPFNGSKILVLGVAYKGNIDDMRESPALKVIEILERKKAEVYYFDPFVSEFSLNGKSYKSIDLDKEVLKKFDGAIITTAHTIGIDYEYIIENLPFVFDTKNVLKNFKNRENIYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDP2</accession>
    <name>B7IDP2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Rubrerythrin, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580345.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1682"/>
    <dbReference type="EnsemblBacteria" id="ACJ76119">
        <property type="protein sequence ID" value="ACJ76119"/>
        <property type="gene ID" value="THA_1682"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1682"/>
    <dbReference type="eggNOG" id="COG1633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125830_0_0_0"/>
    <dbReference type="OMA" id="MEKEHTE"/>
    <dbReference type="OrthoDB" id="1595913at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="167" mass="19887" checksum="CFD9CA6306826373" modified="2009-02-10" version="1">MSIEDILKLAENFEIEGYKFYMSKKEEIKNSLAKEVLEFLKNMEKEHTEYIRMVRKALEENGEIPTASVDTTKDFFKERFTSQKISKTPSEDDIKDLSILRMALLIEKDFVNYYDKAAKRARELENEQLVKILENLKAWEEGHVELVKKMIKEIYEKNRLDLGFYPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDQ2</accession>
    <name>B7IDQ2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MnhB subunit of Na+/H+ antiporter family-like domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1692</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102528.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1692"/>
    <dbReference type="EnsemblBacteria" id="ACJ76129">
        <property type="protein sequence ID" value="ACJ76129"/>
        <property type="gene ID" value="THA_1692"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1692"/>
    <dbReference type="eggNOG" id="COG2111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069132_3_0_0"/>
    <dbReference type="OMA" id="TAVLFDW"/>
    <dbReference type="OrthoDB" id="1836204at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007182">
        <property type="entry name" value="MnhB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04039">
        <property type="entry name" value="MnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="MnhB" evidence="8">
        <location>
            <begin position="112"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="240" mass="25643" checksum="E6446D3CF73E72E1" modified="2009-02-10" version="1">MRRVFSILIVLGLILFFVNSLSILPEYGKVKLSKVSSAYINKSVNGNSEKVTFGKSKDLEDGSANVVTSIVVNYRSFDTLGEVTVLFTSALGVGLILHGYKRKKYGRQPNFILRISVGILLPLILLFGAYIFIHGHLTPGGGFPGGTIIAAGILLLYLSNEEYELSKASKFVESAAGSLYVIVGLTGLAVSGIFLLNFLPTGVVGNLFSAGIVPIVYILIGFKVGAELSGIIADLHREEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDT5</accession>
    <name>B7IDT5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ComEC/Rec2-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1726</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102598.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1726"/>
    <dbReference type="EnsemblBacteria" id="ACJ76162">
        <property type="protein sequence ID" value="ACJ76162"/>
        <property type="gene ID" value="THA_1726"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1726"/>
    <dbReference type="eggNOG" id="COG0658">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_643837_0_0_0"/>
    <dbReference type="OMA" id="ALFRFPW"/>
    <dbReference type="OrthoDB" id="1857516at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004477">
        <property type="entry name" value="ComEC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03772">
        <property type="entry name" value="Competence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00360">
        <property type="entry name" value="ComEC_N-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Competence" evidence="7">
        <location>
            <begin position="170"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="410" mass="47346" checksum="8B0DDD89813CD8FA" modified="2009-02-10" version="1">MYFLIFLSSILGALIAPVFSFPKFVIFLPFLFFKKRECFWILFFFLAFNILSVVNFNGEFEFVGRVIQTNDSYSLVKGDIFYNGKWKKLNVLVGVNKEIPLGDIVYYYGPFDAKKYSYPKVTLDKNRVIVSPYFSLVRNIYTKTENFRKKILNYSKVYYGLFGGKVKDKIYSDSGLYHFFSISGLHISLIFAILTSVLTFFSLSDLSKKFIALMVSFLFLISCGNNLPSLRAFLYLFLVILFEKLIPKMSKIDILSFVGLFFLFFQPFLAYSLSFYMTFFSTFGILSVENNKLKPLAAFLGCAPFLSLFSNVNIFSIIGTFVLIIPFQIILTSLMIAYFFYILNFSLIVDFILKVSLPITKFIEFISGIFSRFPKIHGGIITYFLFSVLFFAFLLHFGQIPYILKSKSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDW7</accession>
    <name>B7IDW7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1763</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102660.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1763"/>
    <dbReference type="EnsemblBacteria" id="ACJ76194">
        <property type="protein sequence ID" value="ACJ76194"/>
        <property type="gene ID" value="THA_1763"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1763"/>
    <dbReference type="eggNOG" id="COG2304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_672553_0_0_0"/>
    <dbReference type="OMA" id="FPIVENA"/>
    <dbReference type="OrthoDB" id="1650501at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="409" mass="47735" checksum="22F0CD3A353DA470" modified="2009-02-10" version="1">MFDFKEMIASELFKSLIKVFTKLEDYEITIENNKLVISSSNIPVNILNSKSRVSILDSNGEKIDINKSPAVIESGKIIFNDLDEKFLEKLKYFIKDLSNEKVIESNFEGEVIERNDKSKIYVKGILVAEEDNLLYSYNIKNPTKNMIKKLENLDLPFKKTAFVYKLKNILLLCENTEIAMKLANEIINSENIHDEIRWKDVQIHALKLLNALNNYLFITKKEIIQYNHLLGNARNSGYKIIEIPEEIKEELLNERDILGNRINTIQNYFEKYSKNFVFEIIDEKKLSEKEKENFQSLKKFLSTIDKDISVLISTNMLERIDSKSFYYIKDNVIYLKRDVLNSFDFTLKVVIEAISKILYPSYSTKDAILSLFVKHLSETKIKETHVSKVESKNSFFSSFINKLRKKSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDW8</accession>
    <name>B7IDW8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1764</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102662.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1764"/>
    <dbReference type="EnsemblBacteria" id="ACJ76195">
        <property type="protein sequence ID" value="ACJ76195"/>
        <property type="gene ID" value="THA_1764"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1764"/>
    <dbReference type="eggNOG" id="COG0628">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031275_8_3_0"/>
    <dbReference type="OMA" id="WRTLTAY"/>
    <dbReference type="OrthoDB" id="944044at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="327" mass="37154" checksum="25B84024CF7CE958" modified="2009-02-10" version="1">MDDKFLILIYFILFLILIKFSPFVFGAVIIGFYFSIVIDVPAKLLSKKLNKNLSKVISYTLMLSLIFYSFITFIPVILEEGKKIFSELSKISINGNPQLPSWILDFLNNSNKQISNFALNVLNQLFSYTPSIITMGILIIVTTLAVSNLKAYLKKNLKSFFVYNPEKGYNFFRSFYKDFERFVSGQVLVALFVGISVGFLCFVFGIKGALFLGILSFITDFIPFLGVIIVSIPMLMLGWTSKGFTGIVLGIIILVIVNQLESWFFAPKIQSSNLKIHWFVLIISLLIFNDFFGFIGILIAIPTILFIKKFWKYYILGGSYGNNRGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE35</accession>
    <name>B7IE35_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Basic membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1831</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BMP lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102779.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1831"/>
    <dbReference type="EnsemblBacteria" id="ACJ76262">
        <property type="protein sequence ID" value="ACJ76262"/>
        <property type="gene ID" value="THA_1831"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1831"/>
    <dbReference type="eggNOG" id="COG1744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038813_0_0_0"/>
    <dbReference type="OMA" id="DYYANDW"/>
    <dbReference type="OrthoDB" id="603910at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003760">
        <property type="entry name" value="PnrA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02608">
        <property type="entry name" value="Bmp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Bmp" evidence="3">
        <location>
            <begin position="20"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="353" mass="39206" checksum="5E5B192E74613CE5" modified="2009-02-10" version="1">MKKLLFLVFILIFSSLFAFKVIMVTDVGGLGDGSFMDGTWSGIVRACQEYGIEYEVIQSKEQSDYISNLSKAAESADVVFAVGFLMSDAFYKVAEQYPETYFVGIDFHQEKDLPNVMTFTFKEQEGSFLTGYLAAGMTKTGKVAIIGGIPIPPVKRYEIGFRTGVKVYNSIHNTNVEVKVVYANSFTDPKKGKELTQALISEGVDIIQQACGGTALGIIEAIKEENLKNVTSKELKELIDYMYLNGGYYMLGGDVEQEWQAPGHILASAIKRVDNASYMGVVKAFTGDWHPGNIELGLKEDGEGISRMPFTKGLVPNDLIYELEYLISLVKRGEFKIPETEDELNALRVKIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEG7</accession>
    <name>B7IEG7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator HTH-type, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1973</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580518.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1973"/>
    <dbReference type="EnsemblBacteria" id="ACJ76394">
        <property type="protein sequence ID" value="ACJ76394"/>
        <property type="gene ID" value="THA_1973"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1973"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_3_1_0"/>
    <dbReference type="OrthoDB" id="1800563at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="114" mass="13693" checksum="2A75C974CA2427E8" modified="2009-02-10" version="1">MIEVIEIIKLFSNETRARILFLISNTEVCNCDIENVLNITQSNVSKHLKQAEFLNLINKRKNSYWTYYSLNQEMLKRYSFINEILKELAKIEPFKNDLKKMNEYLKNPGRCERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEH4</accession>
    <name>B7IEH4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76401">
        <property type="protein sequence ID" value="ACJ76401"/>
        <property type="gene ID" value="THA_1980"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1980"/>
    <dbReference type="HOGENOM" id="CLU_3277931_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="41" mass="4803" checksum="E74D5CCA3ECA9D99" modified="2009-02-10" version="1">MRGMNERTLKAQITYMLLGQQLTMLIDTLTAHPKMIRLIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEI3</accession>
    <name>B7IEI3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase, group 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1989</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580530.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1989"/>
    <dbReference type="EnsemblBacteria" id="ACJ76410">
        <property type="protein sequence ID" value="ACJ76410"/>
        <property type="gene ID" value="THA_1989"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1989"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_645170_0_0_0"/>
    <dbReference type="OMA" id="VEHNFEV"/>
    <dbReference type="OrthoDB" id="539030at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="213"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="435" mass="50504" checksum="41AA070081BF1995" modified="2009-02-10" version="1">MKVALVHYRAGLMDGVSLEMEKWRKVLKRMGHEVDIVAGNNKEGVDVVVPSIGFENPKYRLINKNAFEKLEDFDINGLLNIIFEESDKVYNDIFHVLKKYDVIIPNNIWSLGAFLPSAIALFRFAKEHPEKIFIGHHHDFWWERKYFLNYTDEKIKELLEMYCPPLGDNIKHVVINSLARNALYLKKNVESTIVPNVMDFNEPLFTNEDVNLKIRDAYNISENSIVLLQATRITERKAIELAIELVNQMSKLSKKYVGKKLYNGKIFNGEIILAFSGMCESENYKYKLLDLAFKYGIRTVDLYPNVENKVWTFWDLYSIADAITYPSILEGWGNQLLEAIMVKKPIVLFEYKVFESDIKMSGLKYISLGNSYFKKDGLVNVDEEVEIKAAEELFEILFDKEAYEKTVNENFEIGKKFYSLESLQKIIQGILNDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEJ9</accession>
    <name>B7IEJ9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_5</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_5"/>
    <dbReference type="EnsemblBacteria" id="ACJ74513">
        <property type="protein sequence ID" value="ACJ74513"/>
        <property type="gene ID" value="THA_5"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_5"/>
    <dbReference type="HOGENOM" id="CLU_3349758_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="37" mass="4286" checksum="0BFE041A893A3A0D" modified="2009-02-10" version="1">MLSSLNLSDFYYGRVLSMLINKGLNPILIENSDERRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEK3</accession>
    <name>B7IEK3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chemotaxis protein MotB, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_9</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579291.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_9"/>
    <dbReference type="EnsemblBacteria" id="ACJ74517">
        <property type="protein sequence ID" value="ACJ74517"/>
        <property type="gene ID" value="THA_9"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_9"/>
    <dbReference type="eggNOG" id="COG2885">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016890_13_2_0"/>
    <dbReference type="OMA" id="ISDMMAG"/>
    <dbReference type="OrthoDB" id="1619550at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OmpA-like" evidence="2">
        <location>
            <begin position="73"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="217" mass="25520" checksum="588A4C88F041E0C4" modified="2009-02-10" version="1">MNRKFNPWISYSDLFSALLLIFVLLLSLTLLQYTYAIQNKETRLKEIIGIKAKIVEELVNTFKDLDLEIQVDPNSGAIIINNDILFNYNDYTLSTKGKNILLKVIKTYLNVILKPEYETYIAEFYLEGNSDPTGTYEYNIDLSMKRAFEVFKYLRENLDEIPYQELFFKKLVVSGKGYNNPIIENGKINYTKSRRVELKFTLKDQEMIKEILEIFQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEK5</accession>
    <name>B7IEK5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Purine nucleoside phosphorylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_11</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>adenosine + H(+) + H2O = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:24409"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>adenosine + phosphate = adenine + alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:27642"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:27643"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27646"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:27647"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>phosphate + S-methyl-5'-thioadenosine = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11852"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.28"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:11853"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="5 9">Belongs to the purine nucleoside phosphorylase YfiH/LACC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103135.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_11"/>
    <dbReference type="EnsemblBacteria" id="ACJ74519">
        <property type="protein sequence ID" value="ACJ74519"/>
        <property type="gene ID" value="THA_11"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_11"/>
    <dbReference type="eggNOG" id="COG1496">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065784_0_0_0"/>
    <dbReference type="OMA" id="CFADCVP"/>
    <dbReference type="OrthoDB" id="1393323at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd16833">
        <property type="entry name" value="YfiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003730">
        <property type="entry name" value="Cu_polyphenol_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038371">
        <property type="entry name" value="Cu_polyphenol_OxRdtase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011324">
        <property type="entry name" value="Cytotoxic_necrot_fac-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30616">
        <property type="entry name" value="PTHR30616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02578">
        <property type="entry name" value="Cu-oxidase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64438">
        <property type="entry name" value="SSF64438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00726">
        <property type="entry name" value="TIGR00726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="263" mass="30442" checksum="6751CABA70BBE1F2" modified="2009-02-10" version="1">MQIGNYVCKEINGLFLCRSSLLCQFNKLFHIVTTRKVNKNQFNFDELDMSFNNENFKDHYILLSDNLGLDLNKFIFTNQVHENKVEYVTSKNLKGEFYENVISKTDGLITDEKGIFLVTKYADCMPIIAYDPIKNVVGVAHSGWRGTLIDMPKHLIIKMMNEFGSNPENIFVSIGPSIGPESFEVGKDVAESFAKKFGDMFIMESNDKYFVNLWKIAENQIYEMGVRNIEISLIDTYRNTDYFYSYRKEKTKKRFAVIVGLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEL6</accession>
    <name>B7IEL6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Penicillin-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_22</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579298.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="SMR" id="B7IEL6"/>
    <dbReference type="STRING" id="484019.THA_22"/>
    <dbReference type="EnsemblBacteria" id="ACJ74530">
        <property type="protein sequence ID" value="ACJ74530"/>
        <property type="gene ID" value="THA_22"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_22"/>
    <dbReference type="eggNOG" id="COG0768">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009289_6_0_0"/>
    <dbReference type="OMA" id="EVLPYMN"/>
    <dbReference type="OrthoDB" id="213680at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005543">
        <property type="entry name" value="PASTA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51178">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PASTA" evidence="1">
        <location>
            <begin position="514"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="569" mass="64547" checksum="74D0D875B4B723A8" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEM8</accession>
    <name>UVRC_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UvrABC system protein C</fullName>
            <shortName evidence="1">Protein UvrC</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Excinuclease ABC subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uvrC</name>
        <name type="ordered locus">THA_34</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with UvrB in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UvrC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579308.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IEM8"/>
    <dbReference type="SMR" id="B7IEM8"/>
    <dbReference type="STRING" id="484019.THA_34"/>
    <dbReference type="EnsemblBacteria" id="ACJ74542">
        <property type="protein sequence ID" value="ACJ74542"/>
        <property type="gene ID" value="THA_34"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_34"/>
    <dbReference type="eggNOG" id="COG0322">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014841_3_2_0"/>
    <dbReference type="OMA" id="HIECFDN"/>
    <dbReference type="OrthoDB" id="1036075at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00203">
        <property type="entry name" value="UvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004791">
        <property type="entry name" value="UvrC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001162">
        <property type="entry name" value="UvrC_RNase_H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038476">
        <property type="entry name" value="UvrC_RNase_H_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08459">
        <property type="entry name" value="UvrC_HhH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="SSF82771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00194">
        <property type="entry name" value="uvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50165">
        <property type="entry name" value="UVRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0228">DNA excision</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0267">Excision nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_1000200608" description="UvrABC system protein C">
        <location>
            <begin position="1"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="12"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="1">
        <location>
            <begin position="193"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00203"/>
        </source>
    </evidence>
    <sequence length="551" mass="64177" checksum="DF70697C419DC19B" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IER6</accession>
    <name>B7IER6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tetratricopeptide repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_72</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579331.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_72"/>
    <dbReference type="EnsemblBacteria" id="ACJ74580">
        <property type="protein sequence ID" value="ACJ74580"/>
        <property type="gene ID" value="THA_72"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_72"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_787396_0_0_0"/>
    <dbReference type="OMA" id="NYAPAYE"/>
    <dbReference type="OrthoDB" id="1283603at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="128"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="162"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="196"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="354" mass="41734" checksum="CAB9ECE9D74D0B97" modified="2009-02-10" version="1">MKVEMIVKDKIVTITDESPLYIMLRDLFYGGDWYNMKKDFNSNEFLNEIENLEFIEKNISILNDSFYAPIIWSELVSFFNEKNITPELFQHATVDGLYELSIEYADKDLLGDAKNILEFAIKVDKNFAPAYDFLGSIHIELGNIEEGIKFLNRAVEIDPWLVQSYSTLGEVYYNQGDYEKAISYWLKEIEYSPNDIFTYFMIADAYTRMKNYEKAANILNKLLEIDNNNIIAMYELSQIYREMGKTKEADMVEKEILNAKPIDPNGIEIWAKIKMKYNKYDEIVEVIEPMIDNTIDSLHLKALLVVPYIKLGKIEEARKYYEELKQNDFWYLFGKKEIFDKYLTKKEKELCGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IES1</accession>
    <name>B7IES1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmB</name>
        <name evidence="2" type="ordered locus">THA_77</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103424.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_77"/>
    <dbReference type="EnsemblBacteria" id="ACJ74585">
        <property type="protein sequence ID" value="ACJ74585"/>
        <property type="gene ID" value="THA_77"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_77"/>
    <dbReference type="eggNOG" id="COG0220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077150_0_0_0"/>
    <dbReference type="OMA" id="PWPKAGH"/>
    <dbReference type="OrthoDB" id="1025521at2"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01057">
        <property type="entry name" value="tRNA_methyltr_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="PTHR23417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00091">
        <property type="entry name" value="TIGR00091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="177"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="309" mass="36561" checksum="FB73EBBB689A5E66" modified="2009-02-10" version="1">MIYPEYELKPQFHEEFPLQWKKIFDNNNPLHVELGFGNGEFAAYYTQKHKNINYIGFELSITSMVKAQRKLKRNNVTNAKLILCDARFGLREFFDSNSIEKVIMNFPVPWYKNSQAHRRIIIQDFFETLADVLIENGEFELLTDQEWYAIEAFENAKNSGYFEVFDIEKNPRREFLTRYEKKWIKSNRDIFKLVVKKIKGIKVSRLIGGVNKMPHASGKISEEKIKLLKGKVFKENNKVFVIKNVYKELDSNSHIFKVISSDGDFSQHYFLVLYQKDNENWVIKLDSESNPYRTPAVKWSVKKIMEVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>B7IES2</accession>
    <name>B7IES2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycerol-3-phosphate dehydrogenase [NAD(P)+]</fullName>
            <ecNumber evidence="7">1.1.1.94</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">NAD(P)H-dependent glycerol-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">gpsA</name>
        <name evidence="15" type="ordered locus">THA_78</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>NAD(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>NADP(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:11096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 11">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.94" evidence="7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579336.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_78"/>
    <dbReference type="EnsemblBacteria" id="ACJ74586">
        <property type="protein sequence ID" value="ACJ74586"/>
        <property type="gene ID" value="THA_78"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_78"/>
    <dbReference type="eggNOG" id="COG0240">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033449_0_2_0"/>
    <dbReference type="OMA" id="NRMFGNM"/>
    <dbReference type="OrthoDB" id="1419877at2"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009331">
        <property type="term" value="C:glycerol-3-phosphate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004367">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase [NAD+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00394">
        <property type="entry name" value="NAD_Glyc3P_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10 11" id="KW-0520">NAD</keyword>
    <keyword evidence="3 7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_Gly3P_dh_N" evidence="13">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_Gly3P_dh_C" evidence="14">
        <location>
            <begin position="168"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7 9">
        <location>
            <begin position="243"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 8">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ACJ74586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="322" mass="35898" checksum="6AD23761C38981F2" modified="2009-02-10" version="1">MKFGILGAGSWGCAFANLLVESGHDVLVWARRKEVVDEINNYSKNSYLPNVKLNFKATNDLNEIIEFSEFLVIAIPVQFIRDVIKNIKVWNIKGILNLSKGIEIKTLKRVSEIIEEFTNTPYSILSGPSHAEEVANKIPTAVTVSGSNAKLFQSLISNDYFRVYTSEDIVGVEIAGALKNVMAIAAGILDGLGGWDNSKAALMTRALHEMIKFGKIFGADEKTFFGLPGVGDLMVTCNSRHSRNRMFGELIAKGHTLNELIKNNKMIAEGIYTVKAVVEIAKKKNIEMPIANEVYEILYNNKNPKSSMVELMRRPLKKEWLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IET2</accession>
    <name>B7IET2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ymf77, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_88</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579342.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_88"/>
    <dbReference type="EnsemblBacteria" id="ACJ74596">
        <property type="protein sequence ID" value="ACJ74596"/>
        <property type="gene ID" value="THA_88"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_88"/>
    <dbReference type="HOGENOM" id="CLU_1133171_0_0_0"/>
    <dbReference type="OMA" id="QIVENEF"/>
    <dbReference type="OrthoDB" id="2005164at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="245" mass="29607" checksum="17B600D12CD877F4" modified="2009-02-10" version="1">MKKLVSILLTTFFLLFPYFLFKIDYFNSLKELNFSKQIVENEFKSYSQLVKEYISVKKPDGYVVDNKIYFEGSLYEYNNIKEGFNILTLNNKEELFYITKNNLYKVPGINSTFLFYISTNEKIMNEGYKFKNLQDVFPDIEKNVTYFNGKKVLFKKIKLSNDCYSLVYVLYPKKYLTLYFVFIPTSILIFYFFFFYNREMEKNLNKNIKKFSRSIKILKNIIKNCEHNETLKEEIKELKKILKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEY0</accession>
    <name>B7IEY0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pyrimidine-nucleoside phosphorylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_136</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579369.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_136"/>
    <dbReference type="EnsemblBacteria" id="ACJ74644">
        <property type="protein sequence ID" value="ACJ74644"/>
        <property type="gene ID" value="THA_136"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_136"/>
    <dbReference type="eggNOG" id="COG0213">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025040_0_1_0"/>
    <dbReference type="OMA" id="DVWRRMI"/>
    <dbReference type="OrthoDB" id="1724909at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004645">
        <property type="term" value="F:1,4-alpha-oligoglucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016154">
        <property type="term" value="F:pyrimidine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006206">
        <property type="term" value="P:pyrimidine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006213">
        <property type="term" value="P:pyrimidine nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036566">
        <property type="entry name" value="PYNP-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013102">
        <property type="entry name" value="PYNP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018090">
        <property type="entry name" value="Pyrmidine_PPas_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017872">
        <property type="entry name" value="Pyrmidine_PPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000053">
        <property type="entry name" value="Thymidine/pyrmidine_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10515">
        <property type="entry name" value="PTHR10515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07831">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000478">
        <property type="entry name" value="TP_PyNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00941">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="SSF47648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="SSF52418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54680">
        <property type="entry name" value="SSF54680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02644">
        <property type="entry name" value="Y_phosphoryl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00647">
        <property type="entry name" value="THYMID_PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PYNP_C" evidence="5">
        <location>
            <begin position="345"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="434" mass="47203" checksum="16CDE215DA892502" modified="2009-02-10" version="1">MRPYDVILKKRNGGKLTKEEIEYMIMGYVNGEIPDYQMSAFLMAIYFRHLDKDERAIFTEIMAKSGDMLDLSPIKGVKVDKHSTGGVGDKTTLVVAPIVASLGIPVAKMSGRALGHTGGTIDKLESIPGFKTSLSLEKFFENVNKYKIAVVGQTANLAPADKKIYALRDATATVDEISLIASSIMSKKLAGGADAFVLDVKVGSGAFMKDISSAKELASAMVEIAKSHNKTAVAVLTNMDEPLGTFAGNSLEVLEAINTLKGQGDEKFRTLCLTLSAWMCHLAGLGSYEKCFELSKEALDTGKALEKFREFVKAQEGNEMVVESPEKILPISNKIVEFKASEDGYISSIDTEKVGIACNYLGAGRVKKEDNIDHSVGLEFLKKIGDKVKNGDTLVRLYVSEKSNIDEAMNLLKQAYNISSTKTNPLEIILDIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF02</accession>
    <name>B7IF02_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyruvate carboxylase subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579380.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_159"/>
    <dbReference type="EnsemblBacteria" id="ACJ74666">
        <property type="protein sequence ID" value="ACJ74666"/>
        <property type="gene ID" value="THA_159"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_159"/>
    <dbReference type="eggNOG" id="COG5016">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_4_2_0"/>
    <dbReference type="OMA" id="EAGMISQ"/>
    <dbReference type="OrthoDB" id="361205at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="458" mass="51909" checksum="CEECB3935210CFC2" modified="2009-02-10" version="1">MFVDTTLRDGHQSLIATRMSINDMLPALNDMDDLGFEAMEVWGGATFDVCVRFLNEDPWERIKTIKSHLKKTKTQMLLRGQNLVGYRHYADDVVELFVKKAIENGVDKIRVFDALNDIRNLEKSIEIALANGAHVQGAISYTISPVHTLEYYLNYAQQLVEKGVNSLCIKDMAGLLTPKFSFELVSELKKRFNVPVEVHTHCTAGLGDLNYLAAVEAGADYLDTALSPFALGTSQPPFEAMYYAVREYRNLPDIDWKKISVLVEHFKAVREKHKDYDVMMKSIDYRILVSQVPGGMYSNLVKQLSEQKMLNKLEDVLNEIPRVRKDLGYPPLVTPTSQIVGVQAVLNVLTGERYKKVTNEVKNYVKGMYGKPPAPIDEELKKKILGDEKPIDCRPADLIEPEVERARKEIGVLAETDEELLIYILLGEVGRKYLYNKYQEKIKVDLQLVDELGGGYPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF29</accession>
    <name>B7IF29_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Succinyl-diaminopimelate desuccinylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_188</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579400.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_188"/>
    <dbReference type="EnsemblBacteria" id="ACJ74693">
        <property type="protein sequence ID" value="ACJ74693"/>
        <property type="gene ID" value="THA_188"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_188"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_2_2_0"/>
    <dbReference type="OMA" id="STFEPTM"/>
    <dbReference type="OrthoDB" id="906744at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010182">
        <property type="entry name" value="ArgE/DapE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01910">
        <property type="entry name" value="DapE-ArgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="3">
        <location>
            <begin position="198"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="407" mass="45821" checksum="3D3A83AFAFF1F2C9" modified="2009-02-10" version="1">MKEKIIKRVEEIQEDIKNSMKKFISINSVNPRSGGPGEKEMAEWLQSLIKDWGFDSIERYDAKDDIVPYGFRPNIVARYNGTVGKRTIWIITHMDKVPAGDLSLWENDPFTPVEKDGKIYGRGAEDNGSSLIASLYGLKTIMDLGIRPKDNIALAFVSDEETGSDYGIKYLVKQNLFSKNDLFIVPDSGEPDGSFIEIAEKSILWFKVIVSGKQAHASRPDIAKNAHRYGAKFLTILDEYLNSNYNEEDLLFDYPKSSFEPTKKESNVENVNTIPGTDIFYFDCRILPQYNLEEIFKNIVSLGEKFEKENGVKIQIEKLQFEQAAPPTDKNSEIVVKLINAIKEMRNITPRVGGIGGGTCAAILRNEGLPAAVWATIDETAHQPNEYVVIKNLIEDTKVYAYLIANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF31</accession>
    <name>B7IF31_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-oxoglutarate synthase subunit KorA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103681.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_190"/>
    <dbReference type="EnsemblBacteria" id="ACJ74695">
        <property type="protein sequence ID" value="ACJ74695"/>
        <property type="gene ID" value="THA_190"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_190"/>
    <dbReference type="eggNOG" id="COG0674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_0_1_0"/>
    <dbReference type="OMA" id="DFFQARW"/>
    <dbReference type="OrthoDB" id="360732at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR_N" evidence="1">
        <location>
            <begin position="16"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="PFOR_II" evidence="2">
        <location>
            <begin position="278"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="386" mass="42800" checksum="68E9A1645E6CCD1A" modified="2009-02-10" version="1">MGKLVFWQGNEAAAYGALKAGCRFYAGYPITPSSEIAETMARELPKVNGVFIQMEDEIGSAAAIIGASLAGVKSMTATSGPGFSLMQEALGYAVMTETPCVFVNVMRGGPSTGLPTKPAQGDIMQARWGTHGDHAIIALYPSTVEEVYRYMIEAFNLAEIYRTPVVFLMDETLGHMREAFELPDLKNVEIVNRISEKDLEEEEVFVPYVESEYAEPKPTALVEMGKTRFHVSGLVHDESGFPNAAYETAEKLIKRLSNKIKLHIDEISIYDEYLTEDAEVLVIAYGIVARSAKEAVKIARNDKMRVGLFKPITIWPLPVHELRKRLSKVEAVVIPEMNLGQYGREILRLVKPGTKVKFINKVGGELITPEEILDGINEILTEGYDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF78</accession>
    <name>B7IF78_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579435.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_239"/>
    <dbReference type="EnsemblBacteria" id="ACJ74742">
        <property type="protein sequence ID" value="ACJ74742"/>
        <property type="gene ID" value="THA_239"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_239"/>
    <dbReference type="eggNOG" id="COG0613">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022128_0_0_0"/>
    <dbReference type="OMA" id="HINVYES"/>
    <dbReference type="OrthoDB" id="650608at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POLIIIAc" evidence="1">
        <location>
            <begin position="23"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="746" mass="84877" checksum="25734751836C92E9" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF84</accession>
    <name>B7IF84_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103800.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ74748">
        <property type="protein sequence ID" value="ACJ74748"/>
        <property type="gene ID" value="THA_245"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_245"/>
    <dbReference type="eggNOG" id="ENOG5033UVV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1249072_0_0_0"/>
    <dbReference type="OMA" id="YASWEVR"/>
    <dbReference type="OrthoDB" id="1163889at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="228" mass="24703" checksum="F9C5DB4A26A751C8" modified="2009-02-10" version="1">MGENNILMWVGVGVLAVALVVGVVFLIMPKDQYITAQGIKIWKLPKEAENVQINYSQEAKVAQLAREGRLFLFEGVNVGGGVNMTIAENAARTQALGKIAEFLNAKVETFRKVVEGQLQSVQTNSGNEENNQQLVEAAVSAYKGITKVMAEAIVSGALKYASWEVRKGNVVELHVLYFYDPTNLLSILESQALVSDSVKKLGKQGVDFFKALNSVLDEASKGTPLAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF92</accession>
    <name>B7IF92_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Iron (III) ABC transporter, periplasmic-binding</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579446.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_254"/>
    <dbReference type="EnsemblBacteria" id="ACJ74756">
        <property type="protein sequence ID" value="ACJ74756"/>
        <property type="gene ID" value="THA_254"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_254"/>
    <dbReference type="eggNOG" id="COG0614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_2_5_0"/>
    <dbReference type="OMA" id="WQGINLE"/>
    <dbReference type="OrthoDB" id="1473468at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="37"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="292" mass="32394" checksum="D0C314EE81FF5AB9" modified="2009-02-10" version="1">MKRLAIFLSVLLTTFIFALSIVDDAGRLVSFDKIPERIVSAAPSSTKFLVKLGLGENIVGVTDWDSFNAEKIGNMVPLNIEKIISLNPDIVITFGGFQLPEVQKLEKFGIKALVLNPTTLNDVARDVMLLGNVFGKEQIASEIANKIKQTISDYGVKSSKVPLEKRPKVFFTITVPNNETKDLWTAGTGSYVNELITYAGGVNICAPYSGNNGWFSVNWEFLTYNDPDIIIVASYISADETIKQIKSHEIFKNLKAVKNNKIFVIDGNDISSISPDIVKYLEIFYNFFYGGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFD0</accession>
    <name>GLGA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycogen synthase</fullName>
            <ecNumber evidence="1">2.4.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Starch [bacterial glycogen] synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glgA</name>
        <name type="ordered locus">THA_294</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Synthesizes alpha-1,4-glucan chains using ADP-glucose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18189"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.21" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579478.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFD0"/>
    <dbReference type="SMR" id="B7IFD0"/>
    <dbReference type="STRING" id="484019.THA_294"/>
    <dbReference type="CAZy" id="GT5">
        <property type="family name" value="Glycosyltransferase Family 5"/>
    </dbReference>
    <dbReference type="PRIDE" id="B7IFD0"/>
    <dbReference type="EnsemblBacteria" id="ACJ74794">
        <property type="protein sequence ID" value="ACJ74794"/>
        <property type="gene ID" value="THA_294"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_294"/>
    <dbReference type="eggNOG" id="COG0297">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_18_2_0"/>
    <dbReference type="OMA" id="TWCPWYM"/>
    <dbReference type="OrthoDB" id="83240at2"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033201">
        <property type="term" value="F:alpha-1,4-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004373">
        <property type="term" value="F:glycogen (starch) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009011">
        <property type="term" value="F:starch synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00484">
        <property type="entry name" value="Glycogen_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011835">
        <property type="entry name" value="GS/SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02095">
        <property type="entry name" value="glgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000126108" description="Glycogen synthase">
        <location>
            <begin position="1"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ADP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00484"/>
        </source>
    </evidence>
    <sequence length="485" mass="55785" checksum="22D43DA6A5F7FA6F" modified="2009-02-10" version="1">MKVALVSYEVYPFAKVGGLADVVGALPKYLKNFSVEPIVIMPKHKIVEKNAEKFSYSLRKIKESISVPYLKTDEKFDIYQTFIPGTQIPVYLIANDYYFSAEQVYEGPDLAEQAIYFSAAVFEALKVLGEKVDVLHINDWQTGLVPVYLKTLYKDDEFFSKTVVLLTIHNLGYQGIFDSSYMNFSGLPDYLYNIDGIEFYGKINFLKGGILFSDVINTVSPTYAQEIQTKEYGEKLDGVLRLRSSDLYGILNGIDYDEYNPETDKRIYVNYSLQEIEKKYENKKMLQKELNLPQTNDVPVIGMITRLVDQKGLDILSEVLRYILNMDVQFVLLGTGDKKYEEMFKDIEKEFPDKMSANITFDIVLAQKIYASSDMFLMPSRYEPCGLGQMYSLRYGTIPVVRYTGGLADTVMEYDEEAMKGNGFGFKEYDSAYLLKAVARAVDFFKNKKGHWKKLIENAMKTDLSWERSANEYVKIYNKALNKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFD1</accession>
    <name>B7IFD1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallo-beta-lactamase superfamily, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579479.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_295"/>
    <dbReference type="EnsemblBacteria" id="ACJ74795">
        <property type="protein sequence ID" value="ACJ74795"/>
        <property type="gene ID" value="THA_295"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_295"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_2_6_0"/>
    <dbReference type="OMA" id="HAYNSIF"/>
    <dbReference type="OrthoDB" id="1815576at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039344">
        <property type="entry name" value="MBLAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23200">
        <property type="entry name" value="PTHR23200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="22"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="209" mass="24582" checksum="E21A158A399BA0FD" modified="2009-02-10" version="1">MKMDILLEGGIISVPSRAKATFSTIVLLEDGDRKILIEPGDYVTHSILEEELKKRNLKTEDITDIVLTHFHLDHVYNSIFFKNATVHLHENFLKKNYEKFGMIVGKQYKMIIDSWNAFQTFKDGDILFDKINVYHTPWHSKEHCSFVVDTENMGKVFFAGDIVMTRVEFYDIMRMLRDDDCARFVREMVNKCDYLVLTHDSGVFLENWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFE6</accession>
    <name>B7IFE6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CRISPR type III-B/RAMP module-associated protein Cmr5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_310</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR system Cmr5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579489.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_310"/>
    <dbReference type="EnsemblBacteria" id="ACJ74810">
        <property type="protein sequence ID" value="ACJ74810"/>
        <property type="gene ID" value="THA_310"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_310"/>
    <dbReference type="eggNOG" id="COG3337">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1874450_0_0_0"/>
    <dbReference type="OMA" id="NWYRRYV"/>
    <dbReference type="OrthoDB" id="2022619at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023101">
        <property type="entry name" value="AF1862-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010160">
        <property type="entry name" value="CRISPR-assoc_prot_Cmr5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09701">
        <property type="entry name" value="Cas_Cmr5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158568">
        <property type="entry name" value="SSF158568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01881">
        <property type="entry name" value="cas_Cmr5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="136" mass="16480" checksum="A97F09DF5E399F08" modified="2009-02-10" version="1">MRNKIEIKNFSQNKIKENLKEMESNDELKKSYKSLVKSLGALVLQNGLYASIVFIISKTKDKNNYYYVLKDIQKFLKEYFKDSYVVNNKDIKDIKQEVLEFLESESFKKAYKQFSEQFIEFIKWHRRYVDIYIDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFF6</accession>
    <name>B7IFF6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103949.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_320"/>
    <dbReference type="EnsemblBacteria" id="ACJ74820">
        <property type="protein sequence ID" value="ACJ74820"/>
        <property type="gene ID" value="THA_320"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_320"/>
    <dbReference type="HOGENOM" id="CLU_1864189_0_0_0"/>
    <dbReference type="OMA" id="ISEDFEC"/>
    <dbReference type="OrthoDB" id="2168876at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="137" mass="16059" checksum="DE9FBB78BED5DD8E" modified="2009-02-10" version="1">MKQLIDAIFQKELGDFKSLIGYVYRLKEISKILGFDTLYKSRSTERERRPAIVEDNGKNSLKILFLSTKSYSRIKVDINKFCVISEDFECAELVLKSSYAFNYHKKLVFKLPRKVIEESFVKCGLCRKNIKNFIEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFF7</accession>
    <name>B7IFF7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endoribonuclease Cas2</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cas2</name>
        <name evidence="10" type="ordered locus">THA_321</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas1 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_321"/>
    <dbReference type="EnsemblBacteria" id="ACJ74821">
        <property type="protein sequence ID" value="ACJ74821"/>
        <property type="gene ID" value="THA_321"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_321"/>
    <dbReference type="eggNOG" id="COG1343">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161124_0_1_0"/>
    <dbReference type="OMA" id="WQQNSTF"/>
    <dbReference type="OrthoDB" id="1951170at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09725">
        <property type="entry name" value="Cas2_I_II_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405">
        <property type="entry name" value="PTHR34405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="89" mass="10835" checksum="614328D2A8397A4C" modified="2009-02-10" version="1">MKYLLLVYDVNEKRVNKIHRILKKYLVWQQNSTFEGYLSQSNIKKLFYEVKNKINEEEDSVVVYFFNSNKVFRKVIDGKDKCIFNNKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFF8</accession>
    <name>B7IFF8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endonuclease Cas1</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cas1</name>
        <name evidence="10" type="ordered locus">THA_322</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas2 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103959.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_322"/>
    <dbReference type="EnsemblBacteria" id="ACJ74822">
        <property type="protein sequence ID" value="ACJ74822"/>
        <property type="gene ID" value="THA_322"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_322"/>
    <dbReference type="eggNOG" id="COG1518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052779_2_0_0"/>
    <dbReference type="OMA" id="FIRLECY"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019858">
        <property type="entry name" value="CRISPR-assoc_Cas1_HMARI/TNEAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43219">
        <property type="entry name" value="PTHR43219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03641">
        <property type="entry name" value="cas1_HMARI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="327" mass="38356" checksum="CF8632F3C1CA7DD2" modified="2009-02-10" version="1">METLYIFTDGELKRKNHALYFVPKSKDSNPFFVPLKNISSIMIFSEVSFNKKFLGLLAKEGIPTFFYGYYGDFIGSFYPPENNKTGEMIVLQFQYYYDLSKRLEIAKEILESAVLNMLWVLNGYKEVTNEELNYIVKIKDSLKKQNNIPALMAIEGNIRKAYYKALGKIVKRKGFDFDERLKRPPKDEINALISFGNIILYNTVLAEIYKTSLEPRISFLHETNKRKFSLQLDIAEIFKPIIVDKAILKLVNKKEIKKEDFNFNNNGVFLYEDAKKKFINEIEERLNKKVTIYENQNVTFKTVIRHECYKLIRHLKGVEKFKGFVMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFG4</accession>
    <name>B7IFG4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Endoglucanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_328</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579503.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_328"/>
    <dbReference type="EnsemblBacteria" id="ACJ74828">
        <property type="protein sequence ID" value="ACJ74828"/>
        <property type="gene ID" value="THA_328"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_328"/>
    <dbReference type="eggNOG" id="COG1363">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047249_1_0_0"/>
    <dbReference type="OMA" id="MHTPVEM"/>
    <dbReference type="OrthoDB" id="900854at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="338" mass="37931" checksum="2F9135CE1699ED0C" modified="2009-02-10" version="1">MNTKDLLLKLSSIHGPSGYEHKVTKEIENIMKDLVDEYHYTKVGSLVCVKKGEGKGKIGLLAHVDQLGFVISKITDDGFAYVAGIGGWDPKTVIGQRAKIYSKNREFYGVFGFLAPHLQKAEDRKKIPTFNYLFLDISMNENWKEIQVGDIVMLDEIEGFEKNNLVYAPALDNRASCVSLIKTAEMLKKIKHQFDVYFIFSTQEEIGGPGAPTAAYFADLDYAFVIDVTHGDETIPGFRKIEINKGPVVAVGPVVDKEFNEKVQNVSSQYNIKIQYEPIPRRSGTDTDAVQLVKTGIKTQLISIPLKYMHTPYEKVHVNDVENTAKLMAFTISELEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFH7</accession>
    <name>B7IFH7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable butyrate kinase</fullName>
            <shortName evidence="8">BK</shortName>
            <ecNumber evidence="8">2.7.2.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Branched-chain carboxylic acid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">buk</name>
        <name evidence="10" type="ordered locus">THA_341</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + butanoate = ADP + butanoyl phosphate</text>
            <dbReference type="Rhea" id="RHEA:13585"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58079"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the acetokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.7" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_341"/>
    <dbReference type="EnsemblBacteria" id="ACJ74841">
        <property type="protein sequence ID" value="ACJ74841"/>
        <property type="gene ID" value="THA_341"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_341"/>
    <dbReference type="eggNOG" id="COG3426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048716_0_0_0"/>
    <dbReference type="OMA" id="ANDEGPF"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047761">
        <property type="term" value="F:butyrate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00542">
        <property type="entry name" value="Butyrate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000890">
        <property type="entry name" value="Aliphatic_acid_kin_short-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023865">
        <property type="entry name" value="Aliphatic_acid_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011245">
        <property type="entry name" value="Butyrate_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060">
        <property type="entry name" value="PTHR21060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060:SF3">
        <property type="entry name" value="PTHR21060:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00871">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036458">
        <property type="entry name" value="Butyrate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00471">
        <property type="entry name" value="ACETATEKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02707">
        <property type="entry name" value="butyr_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01075">
        <property type="entry name" value="ACETATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01076">
        <property type="entry name" value="ACETATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="351" mass="38622" checksum="D803DF48B06CD0C7" modified="2009-02-10" version="1">MILVINPGSTSTKVAIFEDDVKKVQETLRHDVEELERFNDLMEQEDFRKEAILKFVESNGYKLEDFDAIACRGGILPPLESGTYIVNEEMVDYLKNKTRVKHASNLAAVIGYSLSGGKIPVYITDPVSVDEMIDEARLSGIPEIERLSLAHALNIKAVAREVANQLGKRYEELNMVVAHLGGGISVTAHEKGRMIDVNNANDEGPFSPERTGELPVGDVAKLCFSGKYTKEELKKMFIGKGGVVAYLGTNDVKKVMEEGTEEEKFIIDAMVYQIAKEIGGMCAVLKGKVDAIVITGGIANNVEVVEKIKSYVFKFAPVFSVPGEFEMEALAKGALRVLKGEEEAKIWGEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFH8</accession>
    <name>B7IFH8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminotransferase, class I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579512.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_342"/>
    <dbReference type="EnsemblBacteria" id="ACJ74842">
        <property type="protein sequence ID" value="ACJ74842"/>
        <property type="gene ID" value="THA_342"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_342"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_6_0"/>
    <dbReference type="OMA" id="MRLNFTY"/>
    <dbReference type="OrthoDB" id="1024978at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="54"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="411" mass="46434" checksum="3D7940CA51086E79" modified="2009-02-10" version="1">MYYSSKYSKLGNNLRSSLIRELLKYASVEGAISFGGGVPDPETFPRHELSKIASEVIENEYGYVLQYSTTEGDPELAKQMLRLLEKLFGISGLDESNIMFTTGSQQALELAGKVFLDEESIAIVENPFYLGAASAFRMRFANFVSVPIEDDGMNVDVLEKKLKELDEKGQIKKVKFIYVIPNFHNPAGATLSLEKRKRIIELAEKYDLLILEDDPYGLLRFEGEHLPSLFKLAGKERVILLNTFSKILAPGLRIGAVIGEKELVRKFVLAKQGTDLCSPALTQRIAARYLERYDLIEQITPTLKLYKSKRDTMMKAFEENFSEINGIKWIYPSGGLFTWVTLPEGFDTLEMFEIAKKRLVFYIPGQAFTPDDSVSSSMRLSFCLPPHEKIIEGVKRLKDVIVEYGKSKGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFI8</accession>
    <name>B7IFI8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein UshA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_358</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-nucleotidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579518.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_358"/>
    <dbReference type="EnsemblBacteria" id="ACJ74852">
        <property type="protein sequence ID" value="ACJ74852"/>
        <property type="gene ID" value="THA_358"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_358"/>
    <dbReference type="eggNOG" id="COG0737">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005854_7_3_0"/>
    <dbReference type="OMA" id="VQPFTNM"/>
    <dbReference type="OrthoDB" id="1884225at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008334">
        <property type="entry name" value="5'-Nucleotdase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="PTHR11575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02872">
        <property type="entry name" value="5_nucleotid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01607">
        <property type="entry name" value="APYRASEFAMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="SSF55816"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="2">
        <location>
            <begin position="24"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="5_nucleotid_C" evidence="3">
        <location>
            <begin position="327"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="517" mass="57251" checksum="27F490422A782A94" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFI9</accession>
    <name>B7IFI9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_359</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579519.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_359"/>
    <dbReference type="EnsemblBacteria" id="ACJ74853">
        <property type="protein sequence ID" value="ACJ74853"/>
        <property type="gene ID" value="THA_359"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_359"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_0"/>
    <dbReference type="OMA" id="RVMHDLR"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="14"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="332"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="366"/>
            <end position="373"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="575" mass="65500" checksum="ABE98CC37A73F7A5" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFK4</accession>
    <name>B7IFK4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_374</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579532.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_374"/>
    <dbReference type="EnsemblBacteria" id="ACJ74868">
        <property type="protein sequence ID" value="ACJ74868"/>
        <property type="gene ID" value="THA_374"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_374"/>
    <dbReference type="eggNOG" id="COG1454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007207_0_4_0"/>
    <dbReference type="OMA" id="LPHTAHY"/>
    <dbReference type="OrthoDB" id="1456634at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd08186">
        <property type="entry name" value="Fe-ADH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045910">
        <property type="entry name" value="AdhA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="PTHR11496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00913">
        <property type="entry name" value="ADH_IRON_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-ADH" evidence="2">
        <location>
            <begin position="18"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="404" mass="44069" checksum="3FC7BAF1A7E3A96C" modified="2009-02-10" version="1">MWESKMDIYNVFELRCKTTCYFGVGAIKKISDILDNLKAQGINNVIFVTDKIAYKVTGAWDVIEPELKAKGFKYVIYEDVSPNPTTAQINEATKLGLQNGAQAVIGIGGGSPIDTAKSVAILLEYPEKTAEELYEGLFTPEKAKPIIAINTTHGTGTEVDRFAVASILEKEYKPAIAYDCIYPLYAIDDPQLMVTLPKGQTVFTSIDAVNHVTEAATTLVASPYSILLAKETIRLIARYLPQAVAHPDDLTARYYLLYASAIAGISFDNGMLHFTHALEHPLSAVKPDLAHGLGLAMLLPAVVKHIYIAKPEVLADIYEPIVPGLKGVPGEAEKIAAGIEKWLFNLGVTEKLEDMGFTENDVEKLTKLALETPSLGLLLSMAPIKADEKTIASIYRDSLKPISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFU3</accession>
    <name>B7IFU3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phospholipase D active site motif domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579597.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_466"/>
    <dbReference type="EnsemblBacteria" id="ACJ74957">
        <property type="protein sequence ID" value="ACJ74957"/>
        <property type="gene ID" value="THA_466"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_466"/>
    <dbReference type="eggNOG" id="COG1502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082411_0_0_0"/>
    <dbReference type="OMA" id="LWKAKRY"/>
    <dbReference type="OrthoDB" id="1881748at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="1">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="1">
        <location>
            <begin position="227"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="290" mass="33758" checksum="D06A9EA66EE3042B" modified="2009-02-10" version="1">MKISSVLIIFLSLVAFSANIYFTEFVDLTDIVIDFINNSNNFLYVSSYSLDNYYVVEAINRLSEKGLDVRVILEVSNKDLKCKVLKDYEKSLHHAKFMVNDNGVIFGSANFTDSGLFEGYNDIVVFEKDKVEDFKNLFLNLWNEGKVSGCKNFFVVGYDDVESKLLEFIQSAKKRIYVCVYALTNQKVFALLKYKESRGVEIKIITDKWFNNSILKKYPIRNIVIVRDKMLHHKFVIVDNSVFLGSVNITVNGLTKNFEMAYISNQFLNSYLKVFDYLWRRYSENKDIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFV3</accession>
    <name>B7IFV3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glucose-1-phosphate adenylyltransferase, GlgD subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">glgD</name>
        <name evidence="7" type="ordered locus">THA_476</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579605.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_476"/>
    <dbReference type="EnsemblBacteria" id="ACJ74967">
        <property type="protein sequence ID" value="ACJ74967"/>
        <property type="gene ID" value="THA_476"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_476"/>
    <dbReference type="eggNOG" id="COG0448">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_14_0_0"/>
    <dbReference type="OMA" id="PRSLMDH"/>
    <dbReference type="OrthoDB" id="1557977at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008878">
        <property type="term" value="F:glucose-1-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011831">
        <property type="entry name" value="ADP-Glc_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005836">
        <property type="entry name" value="ADP_Glu_pyroP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011832">
        <property type="entry name" value="GlgDAde_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523">
        <property type="entry name" value="PTHR43523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523:SF6">
        <property type="entry name" value="PTHR43523:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02092">
        <property type="entry name" value="glgD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00809">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="6">
        <location>
            <begin position="5"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="371" mass="41892" checksum="15BA5F36F15DCC06" modified="2009-02-10" version="1">MKVLGLILAGGKSENLGKLVYKRASAALPIAGKYRAIDFTLSNMVNSGIIKVGVLTQYNPRSLMDHLGSGKEWDLDRKKGGLFILQPYIGFSGEYWYKGTADAIFQNMTILRRGEEDYVLIGSGDHIYKMNYSDLYMYHFSKGADITLVTKELDDTYDMSQYGSVVVDENMKITQFYEKVENPPTRRAFLGIYFINKHLLMELLYSTVPNGGNDLLLDVILPRLDELNVYAYDFKGYWRNIKKGVDEYFKINMDIINKRNVREELFYQNGKVYTKLKDFPPAKFTSNSEVVNSIVADGSIISGSVKNSVLFRGTIVKAGAKIENSIIMQGTVIEEGAVVKNAILDKDCHVREGQVIIGEDELVVLEKRTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFW6</accession>
    <name>B7IFW6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alkaline phosphatase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_489</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104291.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_489"/>
    <dbReference type="EnsemblBacteria" id="ACJ74980">
        <property type="protein sequence ID" value="ACJ74980"/>
        <property type="gene ID" value="THA_489"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_489"/>
    <dbReference type="eggNOG" id="COG1785">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008539_6_2_0"/>
    <dbReference type="OMA" id="NIYAYAN"/>
    <dbReference type="OrthoDB" id="585847at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="PTHR11596"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="436" mass="48028" checksum="648FCC664153F517" modified="2009-02-10" version="1">MKKLLLILLLLVSVFSFSVKNVIYMIGDGMGINHVMLASYLEGRLLEFMKTQNIALVTTHSANSNVTDSAAAGTALFTGFKTNNGMIGILPDGTTVPTIAEIAAKHGVKIGIIATSRITHATPGAVYGHVNSRSNENTLAEQLVNSPITVAFGGGWRHFVATGGKRNDGKDLIKLAKEKGFDYITTKEQLLNYNGKKVIGLFNSSHLPAYTDRVNEPTLSEMTKKALEILSKDNSPFFLMIEGSQIDWEAHGNDPYGVWKEIIEFEEAFKVAMEFLKQNPDTLIIVTADHETGGLGLSSGGYYFDVEMIRKFSKNTDFISKESNKDKNKLKELIKKYYQLELTDEDLELFDSLVKEQGNSYYAFSNAIGRLVSKKAHLGWTTYDHTGAPVALYAFGQGADNFKGLIDNTEIPRIIARLAGYPLTFPTYKLPVSGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFZ0</accession>
    <name>B7IFZ0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Purine-binding chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100661.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_515"/>
    <dbReference type="EnsemblBacteria" id="ACJ75004">
        <property type="protein sequence ID" value="ACJ75004"/>
        <property type="gene ID" value="THA_515"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_515"/>
    <dbReference type="eggNOG" id="COG0835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048995_3_1_0"/>
    <dbReference type="OMA" id="ERTIDTC"/>
    <dbReference type="OrthoDB" id="1711729at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039315">
        <property type="entry name" value="CheW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617">
        <property type="entry name" value="PTHR22617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="151" mass="17426" checksum="F6AAFD54D0840012" modified="2009-02-10" version="1">MELKVLTFVLGDEEFAIDIMKVDRVKEYEKTTKLPNSKDYVEGIINLMGEVIPIINLRKKFMLEDFEDKEKSKIIVIRFEENGKKMGFLVDDVKEVITLSGDKIDQTPEYSGLSAEFLLGIAKLENRMILILDVEKVLKKEEKLAIENIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFZ4</accession>
    <name>B7IFZ4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyruvate synthase subunit PorA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_519</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100656.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_519"/>
    <dbReference type="EnsemblBacteria" id="ACJ75008">
        <property type="protein sequence ID" value="ACJ75008"/>
        <property type="gene ID" value="THA_519"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_519"/>
    <dbReference type="eggNOG" id="COG0674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002569_5_0_0"/>
    <dbReference type="OMA" id="WNDQQDS"/>
    <dbReference type="OrthoDB" id="360732at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR_N" evidence="1">
        <location>
            <begin position="19"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="PFOR_II" evidence="2">
        <location>
            <begin position="265"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="392" mass="43413" checksum="35428A700B4B70E8" modified="2009-02-10" version="1">MPEVKNRQAVTGAQAIANAMRQINPDVVAAYPITPQTPVVEYFAQFVADGAVETEMIPVESEHSAMSAVVGAAAAGARAMTATAANGLALMHEIVYIAASSRLPIVMAVANRALSGPINIHCDHSDAMAERDSGWIQLWAENAQEAYDFTLMAIRIAEHEDVRLPVMVNFDGFIISHGVEVVDFLDDEVARKFVGEPKVMYPLLDTEHPVTHGPLDLYDYYFEHKRQQIEAMKHVKKVFKEVAEEFAKISGRKYDLLDKYKVDDAEYIMIALGSTAGTIKYTVDLLREEGHKVGVIKPQIFRPFPKNELQELLNGRKGVIVLDRAASFGAEAPLYEAVKSALYEVAVKPQIGSYVYGLGGRDIKIEHIRKAFEDAFSGNLIADEERYLGLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG08</accession>
    <name>B7IG08_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar hook-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_534</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bacterial flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579637.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_534"/>
    <dbReference type="EnsemblBacteria" id="ACJ75022">
        <property type="protein sequence ID" value="ACJ75022"/>
        <property type="gene ID" value="THA_534"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_534"/>
    <dbReference type="eggNOG" id="COG1256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012762_1_1_0"/>
    <dbReference type="OMA" id="NLHFIEQ"/>
    <dbReference type="OrthoDB" id="1815809at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009424">
        <property type="term" value="C:bacterial-type flagellum hook"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002371">
        <property type="entry name" value="FlgK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30033">
        <property type="entry name" value="PTHR30033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01005">
        <property type="entry name" value="FLGHOOKAP1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02492">
        <property type="entry name" value="flgK_ends"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0282">Flagellum</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="8">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="9">
        <location>
            <begin position="789"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="143"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="847" mass="95010" checksum="FA78E587C2F26F46" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG15</accession>
    <name>ISPE_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-diphosphocytidyl-2-C-methyl-D-erythritol kinase</fullName>
            <shortName evidence="1">CMK</shortName>
            <ecNumber evidence="1">2.7.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispE</name>
        <name type="ordered locus">THA_541</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.148"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. IspE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.148" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100610.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IG15"/>
    <dbReference type="SMR" id="B7IG15"/>
    <dbReference type="STRING" id="484019.THA_541"/>
    <dbReference type="EnsemblBacteria" id="ACJ75029">
        <property type="protein sequence ID" value="ACJ75029"/>
        <property type="gene ID" value="THA_541"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_541"/>
    <dbReference type="eggNOG" id="COG1947">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053057_2_0_0"/>
    <dbReference type="OMA" id="DGYHEIN"/>
    <dbReference type="OrthoDB" id="1938933at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00094"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050515">
        <property type="term" value="F:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00061">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004424">
        <property type="entry name" value="IspE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43527:SF2">
        <property type="entry name" value="PTHR43527:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010376">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00154">
        <property type="entry name" value="ispE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000116936" description="4-diphosphocytidyl-2-C-methyl-D-erythritol kinase">
        <location>
            <begin position="1"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00061"/>
        </source>
    </evidence>
    <sequence length="275" mass="30814" checksum="9FA85D8DCEFB9579" modified="2009-02-10" version="1">MEQNGSTVVRAYAKINLSLDVVKKRDDGYHEINSLFQNISLYDRLSIVKIDRGLEIKSNVEIENNILYKVWDLFCEKYKEPEFGFRVILEKNIPMEAGLGGGSADAAALLFFLGKTFKIPTSELLNLAIKIGSDVPFFLIGGTAVVKGKGEKIEPLPALKGYYVDLLTSENGISTKEAYSLLNPSLFNRAPCSPYVLYEAYKNRNAGEIKRCTYNIFEKVVANNYKEIMANIKRLRRTHITAALTGSGSAVFGVSFRDGKYKFVSRGVEYEETKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG36</accession>
    <name>B7IG36_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100576.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_563"/>
    <dbReference type="EnsemblBacteria" id="ACJ75050">
        <property type="protein sequence ID" value="ACJ75050"/>
        <property type="gene ID" value="THA_563"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_563"/>
    <dbReference type="eggNOG" id="COG5336">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137927_4_0_0"/>
    <dbReference type="OMA" id="KWTFKNG"/>
    <dbReference type="OrthoDB" id="1979701at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032820">
        <property type="entry name" value="ATPase_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09527">
        <property type="entry name" value="ATPase_gene1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="83" mass="9591" checksum="E3AA5BAF55F8C25A" modified="2009-02-10" version="1">MKKDNVVKELNKLNLLTLFGATVIGNVVVGYFIGKWLDKIFEKDKLFVIIFLFFGAISGIYNAIRQLLKEVEKVDKNEKRKNN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG42</accession>
    <name>ATPA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit alpha</fullName>
            <ecNumber evidence="1">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F1 sector subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpA</name>
        <name type="ordered locus">THA_569</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100563.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IG42"/>
    <dbReference type="SMR" id="B7IG42"/>
    <dbReference type="STRING" id="484019.THA_569"/>
    <dbReference type="PRIDE" id="B7IG42"/>
    <dbReference type="EnsemblBacteria" id="ACJ75056">
        <property type="protein sequence ID" value="ACJ75056"/>
        <property type="gene ID" value="THA_569"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_569"/>
    <dbReference type="eggNOG" id="COG0056">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010091_2_1_0"/>
    <dbReference type="OMA" id="LQAPGVM"/>
    <dbReference type="OrthoDB" id="837522at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18113">
        <property type="entry name" value="ATP-synt_F1_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01132">
        <property type="entry name" value="F1_ATPase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01346">
        <property type="entry name" value="ATP_synth_alpha_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000793">
        <property type="entry name" value="ATP_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038376">
        <property type="entry name" value="ATP_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033732">
        <property type="entry name" value="ATP_synth_F1_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005294">
        <property type="entry name" value="ATP_synth_F1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00306">
        <property type="entry name" value="ATP-synt_ab_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039088">
        <property type="entry name" value="F_ATPase_subunit_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00962">
        <property type="entry name" value="atpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_1000143448" description="ATP synthase subunit alpha">
        <location>
            <begin position="1"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="170"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Required for activity" evidence="1">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01346"/>
        </source>
    </evidence>
    <sequence length="507" mass="55883" checksum="B15B62760DE0452D" modified="2009-02-10" version="1">MRINPGEIVKVLESKIEGFKEEINLEDVGKVIQVGDGIARAYGLNNVMANEMVEFVETGTIGVAFNLEEDNVGIIILGDYKGIKEGHTVRRLKKIMQVPVGEALLGRVVNPLGEPVDGLGPIEAKEFRDVEVKAPGVIYRKPVDTPLQTGIKIIDALIPIGRGQRELIIGDRQTGKTAIAIDTIINQKGKGVYCVYVAIGQKASAVARLVSKLKEAGAMEYTTVVVASAADNAALQYIAPYAGCAMGEYFLYNGKDALVIYDDLSKHAVAYRQLSLLLRRPPGREAYPGDVFYLHSRLLERAARLDEKYGGGSLTALPIIETQANDISAYIPTNVISITDGQIYLEPSLFYAGQRPAVNIGLSVSRVGGAAQIKAMKKVAGSLKLDLAQYQELETFAQFATELDPATQAQITRGQRLMELMKQEQYAPMEVEEQVAVLYAGINGYLDDLEVEKVRLFEKKLIEFLKDKKSEILNKIREEKDLSEETEKMLKEAIEEFKSEFVKVYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG61</accession>
    <name>B7IG61_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Riboflavin biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="11">Riboflavin kinase</fullName>
                <ecNumber evidence="11">2.7.1.26</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="11">Flavokinase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="11">FMN adenylyltransferase</fullName>
                <ecNumber evidence="11">2.7.7.2</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="11">FAD pyrophosphorylase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="11">FAD synthase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="13" type="primary">ribF</name>
        <name evidence="13" type="ordered locus">THA_588</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 11">
            <text>ATP + FMN + H(+) = diphosphate + FAD</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + riboflavin = ADP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14357"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 11">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ribF family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.26" evidence="11"/>
    <dbReference type="EC" id="2.7.7.2" evidence="11"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579663.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_588"/>
    <dbReference type="EnsemblBacteria" id="ACJ75075">
        <property type="protein sequence ID" value="ACJ75075"/>
        <property type="gene ID" value="THA_588"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_588"/>
    <dbReference type="eggNOG" id="COG0196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048437_0_2_0"/>
    <dbReference type="OMA" id="HRGHQAI"/>
    <dbReference type="OrthoDB" id="1504821at2"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00406"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02064">
        <property type="entry name" value="FAD_synthetase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015864">
        <property type="entry name" value="FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023468">
        <property type="entry name" value="Riboflavin_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002606">
        <property type="entry name" value="Riboflavin_kinase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015865">
        <property type="entry name" value="Riboflavin_kinase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749">
        <property type="entry name" value="PTHR22749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06574">
        <property type="entry name" value="FAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01687">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004491">
        <property type="entry name" value="FAD_Synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00904">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="SSF82114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00083">
        <property type="entry name" value="ribF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 11" id="KW-0288">FMN</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavokinase" evidence="12">
        <location>
            <begin position="160"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR004491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ75075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="289" mass="33327" checksum="4C507508228D0587" modified="2009-02-10" version="1">MRVVTVGVFDGVHKGHQEILNSLKNLSKEYNASPEIYTIVFPMEYYTGNFDGLLISLEDRITLLEMYGNVYTLNLQEIKDLSPYDFFDFISKDTKAIVVGQDFRFGKNAAGNVNLLENLSKEKNINLKVVADLIIDGKRVSSTLIRKLLKEGNIQKANYLLGRNYPIYGKVYKDKQLGRKLGFPTANIRRSKELITPKFGVYLCKVYTPKLHFGLMNIGLRPTVEKTKSVKYEVYILDFNEDLYGKEIRVELLEFLREETTFESIDALIEQMKNDEKTARKLLEEKYGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG88</accession>
    <name>B7IG88_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">sn-glycerol-3-phosphate transport system permease protein UgpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_616</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579684.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_616"/>
    <dbReference type="EnsemblBacteria" id="ACJ75102">
        <property type="protein sequence ID" value="ACJ75102"/>
        <property type="gene ID" value="THA_616"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_616"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_0"/>
    <dbReference type="OMA" id="IIATMQV"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="111"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="64"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="288" mass="32917" checksum="A9D16D020C276D88" modified="2009-02-10" version="1">MRKLTPYLLLVPTFLIIILFIYFPAFNSFKLSFFQESFFGDKSIFVGLDNYIDLFTDSEYFKVLKTTFIYSLSSVGITIFLAFLISQLLVKNLPGTRIFRTLMFSPYAVSPAISATLWSMMFTPTAGLLSYLFQVIFHIDVDWLTTVPYAMIALIAATVWKMLPFDLIFYIAALQNIPDSILESSLIDGASSWTRMWKIKFPLVTPITFYLFIMNFTTTMFSSFGIIDIMTRGGPLGSTTTMIYRLYLDGFAFQDNGLAAAESVVMFGVMSIITYLYFRFVEKGVHYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG91</accession>
    <name>B7IG91_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_619</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_619"/>
    <dbReference type="EnsemblBacteria" id="ACJ75105">
        <property type="protein sequence ID" value="ACJ75105"/>
        <property type="gene ID" value="THA_619"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_619"/>
    <dbReference type="eggNOG" id="ENOG502ZWC9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2060331_0_0_0"/>
    <dbReference type="OMA" id="HELLHWV"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="132" mass="15893" checksum="B699865F771E2C59" modified="2009-02-10" version="1">MFEKQIPDLVLIKLDEFLREASITIPKRPVVIFESRSLKEFNKITGMPYSIGGVYFDFKIVLQPINILKKKGVYEKVLLHELLHWVLYGLDEKYQEGLIYWWLREYDRKEVEDFLNVFDGNLHNFIISHWNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGG4</accession>
    <name>B7IGG4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_707</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579743.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_707"/>
    <dbReference type="EnsemblBacteria" id="ACJ75178">
        <property type="protein sequence ID" value="ACJ75178"/>
        <property type="gene ID" value="THA_707"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_707"/>
    <dbReference type="eggNOG" id="COG1233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_510694_0_0_0"/>
    <dbReference type="OMA" id="FKRISHG"/>
    <dbReference type="OrthoDB" id="418913at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amino_oxidase" evidence="1">
        <location>
            <begin position="23"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="508" mass="58200" checksum="705070FE7677A1D4" modified="2009-02-10" version="1">MKKVVIVGGGISGLTAGCFSQGDITIIEKNEKCGGLVNSFVKDGFRFEGGVRALLNSGIIFPMLEKLNLALDVLPNYVSVGVEDKIIDVKSKENIKDYEIMLKSLYPENTNEISNFIKVVKKMVDYIEILYSVKNPLFADKSEYFKYLPWILKALKVFMAMKNMNIPVEKYLKKFFENNSLIDIISQHFFKNIPAFYALSYFYIYTDYFYLKGGLGTFPKKLEEYFLKEKGEILYNTKVVSVDVEKKVVLDQNGKKYPYDILIWAADLKELYKMVNLTSRKFLKEKEKILNGKPAESVYTLYLAVDVPPEEFKRISHGHFFYAPCKKGLGDIHRKEIKNLISYGTKKDILNWIKRFVKYNTFEISIPVLKDETAAPKGKTGVIVSVLLDYEIVKKVYDEGWYEEFKEKMDEAFIDVLSDSIYPFLKNNILFKFSSSPISIQNYANTSDGSIIGWGFEEKAPIKNNFQKAALTSIPHVYKAGKWAYTPSGVPMAILTGKIASKYTWGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGK3</accession>
    <name>B7IGK3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_751</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579776.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_751"/>
    <dbReference type="EnsemblBacteria" id="ACJ75217">
        <property type="protein sequence ID" value="ACJ75217"/>
        <property type="gene ID" value="THA_751"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_751"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078424_0_1_0"/>
    <dbReference type="OMA" id="WLNLLQF"/>
    <dbReference type="OrthoDB" id="1450389at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="251" mass="28522" checksum="A27CBAE7D2F73448" modified="2009-02-10" version="1">MVYLNVFKGTVLRVISELKRYYLNTISFFTIMTMLFYFMVLGITKFGNPANLGESIQALAIGYIVWFTFLGTITDLSWTIINEMSIGLIEQAFISPVGPSTIYLFYQFSNFLIMIPFEFLILIVVFKIAGIPLIIPMSFIITLILLMLQGYGIGLILAGITLRFKRTQALLNIAQFAIIGLLLGDYKGIVKYIVPANGYMKILSNLIEGSSVRFNDWIYMITSSLLYIFVGIILFNYFADVVRKRGEISQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGK4</accession>
    <name>B7IGK4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_752</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579777.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_752"/>
    <dbReference type="EnsemblBacteria" id="ACJ75218">
        <property type="protein sequence ID" value="ACJ75218"/>
        <property type="gene ID" value="THA_752"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_752"/>
    <dbReference type="eggNOG" id="COG4555">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_0"/>
    <dbReference type="OMA" id="SFYKKDC"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="311" mass="35283" checksum="CD404B57804B71E3" modified="2009-02-10" version="1">MLQVKNLKKTYVSKGKKVEAVKGITFETEKGEIFAILGPNGAGKTTTIKSILRLIIPDEGEIYINGIDVHKHPSHALKYVSAVLEGNRNIHWKMTVYENLKYFGYIRGLGGKYLKSRISEILEFVELTEKKNELAGKLSRGMQQRLAIGIALLPDTPLILLDEPTLGLDVESSLKVRQMLEKLTKDGKTILLSTHDMQLVEKVANHVLIINKGRVVVSDKKEKLLDAFKKKRFKISFLSNNGYKELEKFGTVVEENGEKILNIQIDNMEQLYEVLEHFKSKNIEIKRLESVMVNFEEVFVNIVRGDENGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGN5</accession>
    <name>B7IGN5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Hydrogenase-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_787</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579795.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_787"/>
    <dbReference type="EnsemblBacteria" id="ACJ75249">
        <property type="protein sequence ID" value="ACJ75249"/>
        <property type="gene ID" value="THA_787"/>
    </dbReference>
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    <dbReference type="eggNOG" id="COG1905">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3383">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018240_2_1_0"/>
    <dbReference type="OMA" id="CGDCVRM"/>
    <dbReference type="OrthoDB" id="323168at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009016">
        <property type="entry name" value="Fe_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003149">
        <property type="entry name" value="Fe_hydrogenase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036991">
        <property type="entry name" value="Fe_hydrogenase_ssu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02256">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00902">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53920">
        <property type="entry name" value="SSF53920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="8">
        <location>
            <begin position="76"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="133"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="177"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="658" mass="73474" checksum="A1F747F81B3DC49F" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGP7</accession>
    <name>B7IGP7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PhoH family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_799</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579804.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_799"/>
    <dbReference type="EnsemblBacteria" id="ACJ75261">
        <property type="protein sequence ID" value="ACJ75261"/>
        <property type="gene ID" value="THA_799"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_799"/>
    <dbReference type="eggNOG" id="COG1875">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022283_2_1_0"/>
    <dbReference type="OMA" id="KMSPWAQ"/>
    <dbReference type="OrthoDB" id="445854at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003714">
        <property type="entry name" value="PhoH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02562">
        <property type="entry name" value="PhoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PINc" evidence="3">
        <location>
            <begin position="3"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="410" mass="46697" checksum="022B170A19E71BA1" modified="2009-02-10" version="1">MVKNFILDTNVLIHDPQSIYSFEDNNVIIPLPVLEELDNLKRHSGGLGKFARDAIRELDNLRKLGKLSEGIKLKNGGTLKVISLKKSDHENIDFLYEKYIDNWILVYTLHVMKNSKNPTFLVSKDINLRVKADALGIPSQDYLTDRSDLETLNPGYFETSNVDMLDKSNLFPNSYIISRDKYFRYDGENLLEMKKHIQAWNVKPRNREQFFAMDALLNDDIKLVSLIGIAGTGKTFITLACALEKTVTEQKYEKIIVARPLVAMGGKDLGYLPGNIEEKMKPWMSPIYDNLEYLFKISNINMKEFMKKEIIDIEALTYIRGRSIPNQFIIIDEAQNLTPHEIKTILTRAGENTKVVLLGDPYQIDTPYLDKDSNGLVYAATKFLNSKLSAHVVLKKGERSLLASEAANLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGQ3</accession>
    <name>RL32_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmF</name>
        <name type="ordered locus">THA_805</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100388.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IGQ3"/>
    <dbReference type="SMR" id="B7IGQ3"/>
    <dbReference type="STRING" id="484019.THA_805"/>
    <dbReference type="EnsemblBacteria" id="ACJ75267">
        <property type="protein sequence ID" value="ACJ75267"/>
        <property type="gene ID" value="THA_805"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_805"/>
    <dbReference type="eggNOG" id="COG0333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129084_1_3_0"/>
    <dbReference type="OMA" id="HRACRHC"/>
    <dbReference type="OrthoDB" id="2092354at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00340">
        <property type="entry name" value="Ribosomal_L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002677">
        <property type="entry name" value="Ribosomal_L32p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01783">
        <property type="entry name" value="Ribosomal_L32p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01031">
        <property type="entry name" value="rpmF_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000120184" description="50S ribosomal protein L32">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00340"/>
        </source>
    </evidence>
    <sequence length="60" mass="6803" checksum="CF4F8D8859AEFDC7" modified="2009-02-10" version="1">MAVPKQKRSRSRTHHKRAKIYRAFSVPVSVCPNCGAPKLPHRVCLNCGHYGKKQVFEVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGR0</accession>
    <name>B7IGR0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cell shape-determining protein MreB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">mreB</name>
        <name evidence="3" type="ordered locus">THA_812</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Forms polymers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="2">Membrane-associated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the FtsA/MreB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579814.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_812"/>
    <dbReference type="EnsemblBacteria" id="ACJ75274">
        <property type="protein sequence ID" value="ACJ75274"/>
        <property type="gene ID" value="THA_812"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_812"/>
    <dbReference type="eggNOG" id="COG1077">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052037_0_0_0"/>
    <dbReference type="OMA" id="MDIFQPS"/>
    <dbReference type="OrthoDB" id="410781at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10225">
        <property type="entry name" value="MreB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02207">
        <property type="entry name" value="MreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004753">
        <property type="entry name" value="MreB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01652">
        <property type="entry name" value="SHAPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00904">
        <property type="entry name" value="mreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0133">Cell shape</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="156"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="204"/>
            <end position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="284"/>
            <end position="287"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="335" mass="35417" checksum="7C5345252AB8CBF7" modified="2009-02-10" version="1">MGKNDLGIDLGTANFLVYQKGKGIVLYQPSVVAISKKTGKIIAIGEEAKEMLGKTPEDTITAVRPMKDGVIADYTIISEVLKIFIKKVTKGFLFKPNMVIGIPAKTTTVEKRAVFDAASSAGAKKVFVVSEPLAAAIGAGIDVTKPEGNIIIDIGGGTTDIAVISLGGVVVGDSVKLAGDAMDDAIVKSVRKLLGLIIGDSTAEEIKKRIGKAHPDIEDFEMEVKGRDAVTGLPRTDKLNSSQVYGMLKPIIESLISRIKVVLEKTPPELSADIMEKGIVITGGGALLRGIDKAIYDEIGVPCKVAEDPLTCVARGTGILLDDEELLNEVAETYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGZ5</accession>
    <name>B7IGZ5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100762.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_900"/>
    <dbReference type="EnsemblBacteria" id="ACJ75359">
        <property type="protein sequence ID" value="ACJ75359"/>
        <property type="gene ID" value="THA_900"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_900"/>
    <dbReference type="HOGENOM" id="CLU_2107844_0_0_0"/>
    <dbReference type="OMA" id="NFHRYIF"/>
    <dbReference type="OrthoDB" id="2042433at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="116" mass="13555" checksum="9B1760B9C99852AB" modified="2009-02-10" version="1">MITFEKINIELLKRFLGDSINLNVSQKGNKFFCDSSKTCSIVKKDDAIVIELEEEKYLISKSSGNYYLLDYKSADNFHRYIFNIDGEKALFVFKKALNEEEILHLLLSIFFIEKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH27</accession>
    <name>B7IH27_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_932</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579887.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_932"/>
    <dbReference type="EnsemblBacteria" id="ACJ75391">
        <property type="protein sequence ID" value="ACJ75391"/>
        <property type="gene ID" value="THA_932"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_932"/>
    <dbReference type="eggNOG" id="ENOG50343AR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109404_0_0_0"/>
    <dbReference type="OMA" id="WYYSLIA"/>
    <dbReference type="OrthoDB" id="1922140at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="209" mass="23223" checksum="D84D1462D5139471" modified="2009-02-10" version="1">MVELEHYLRPLFDYWYYSLIAGIFLLFAAKFVEKLSVAIFGFIIGINILFPAIVERVPQIKEWLSNPTYYQISMVVFGVITAAVLYAIYKSITFIFGFLVVGALGYYIANFIIGYFGITLSFEPLYLYAGVAIVLGIFGGIVTYKKSAEVIGILSILVGAGTISAVIIGFILKGDFSKIDEPLFSSIAIVIFLILVVLGFVINFKKKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH46</accession>
    <name>B7IH46_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sodium/phosphate symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_951</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579897.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_951"/>
    <dbReference type="EnsemblBacteria" id="ACJ75410">
        <property type="protein sequence ID" value="ACJ75410"/>
        <property type="gene ID" value="THA_951"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_951"/>
    <dbReference type="eggNOG" id="COG0306">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015355_0_0_0"/>
    <dbReference type="OMA" id="RMFVAWG"/>
    <dbReference type="OrthoDB" id="1329125at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PTHR11101"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="306" mass="32692" checksum="A9B7A0DAB4ACA167" modified="2009-02-10" version="1">MYVVFAIFFGMLLGSNDSANIFGPSVENGVLKYKTVTKVAALCVLLGALIGGEKGIRTISNFSNMSIYFAAITVFSAALTMLILSKIGIPSSSSQAIVGAILAVGILNNNVNWKILVKLVIAWVTTPVGGIIFGFLLYRILSVPFNKIRSLWVREKVLVISTLLVGAYGSYALGANNVANITGVFANQIGIRMAALVGGLSIAAGALFSNKRVMYTVSKKIIELDYFSSAISILGQSITVWIYSVIGIPVSVSQAIVGAVIGTGLARGSKLTNKKIIFQIITTWIATPLFSGLFTIIVYYFSKIFF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH59</accession>
    <name>EFP_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor P</fullName>
            <shortName evidence="1">EF-P</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">efp</name>
        <name type="ordered locus">THA_964</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the elongation factor P family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100925.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IH59"/>
    <dbReference type="SMR" id="B7IH59"/>
    <dbReference type="STRING" id="484019.THA_964"/>
    <dbReference type="EnsemblBacteria" id="ACJ75423">
        <property type="protein sequence ID" value="ACJ75423"/>
        <property type="gene ID" value="THA_964"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_964"/>
    <dbReference type="eggNOG" id="COG0231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074944_0_1_0"/>
    <dbReference type="OMA" id="WSVVEFQ"/>
    <dbReference type="OrthoDB" id="1260763at2"/>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04470">
        <property type="entry name" value="S1_EF-P_repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05794">
        <property type="entry name" value="S1_EF-P_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00141">
        <property type="entry name" value="EF_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015365">
        <property type="entry name" value="Elong-fact-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020599">
        <property type="entry name" value="Transl_elong_fac_P/YeiP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013185">
        <property type="entry name" value="Transl_elong_KOW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001059">
        <property type="entry name" value="Transl_elong_P/YeiP_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013852">
        <property type="entry name" value="Transl_elong_P/YeiP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011768">
        <property type="entry name" value="Transl_elongation_fac_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30053">
        <property type="entry name" value="PTHR30053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01132">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08207">
        <property type="entry name" value="EFP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09285">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005901">
        <property type="entry name" value="EF-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01185">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00841">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00038">
        <property type="entry name" value="efp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01275">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000117906" description="Elongation factor P">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00141"/>
        </source>
    </evidence>
    <sequence length="185" mass="20864" checksum="2E13693E00B3A3BA" modified="2009-02-10" version="1">MVDVGSLSKGMYIKYEGEIYRVIDVNKHFRARGSGLIRTKLKNMSTGLVREVNFNSGEKVEEAEITFRKASYIYNDGEKYYFMDNETFEQYGIPVSDIEEEKNYLVENTEVDLIMHDGKPIGIQLPTSVVLEVVETEPGFKGDTVSGGGKPAVLETGLKITVPFFVEKGQKVRVDTRTGEYIERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH69</accession>
    <name>B7IH69_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_974</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100942.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_974"/>
    <dbReference type="EnsemblBacteria" id="ACJ75433">
        <property type="protein sequence ID" value="ACJ75433"/>
        <property type="gene ID" value="THA_974"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_974"/>
    <dbReference type="eggNOG" id="COG1728">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121413_2_0_0"/>
    <dbReference type="OMA" id="FFQDQRR"/>
    <dbReference type="OrthoDB" id="1453685at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005585">
        <property type="entry name" value="DUF327"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024042">
        <property type="entry name" value="TM1646-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03885">
        <property type="entry name" value="DUF327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158397">
        <property type="entry name" value="SSF158397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="154" mass="17622" checksum="DA353875E6CBB071" modified="2009-02-10" version="1">MRIEPTGDPKIKNENIKKKKGLKGKHKVSFSSETPGFFDVLLDVEEEQINQELERIVNDILEAGNDFVRSPTPDSLRKYKEKIKKFLKLIEKKMYKLGGKMDYATNTPRLHVIVEEIDSKLQNLAEKLISSEAGTINYAAKVEEINGLILDLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH72</accession>
    <name>B7IH72_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATPase component of ABC transporters with duplicated ATPase domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_977</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579910.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_977"/>
    <dbReference type="EnsemblBacteria" id="ACJ75436">
        <property type="protein sequence ID" value="ACJ75436"/>
        <property type="gene ID" value="THA_977"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_977"/>
    <dbReference type="eggNOG" id="COG0488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_36_0_0"/>
    <dbReference type="OMA" id="DKMFLDN"/>
    <dbReference type="OrthoDB" id="1112046at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="2"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="282"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="208"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="493"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="314"/>
            <end position="321"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="580" mass="67805" checksum="E9C97FAF38C31BE9" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH77</accession>
    <name>B7IH77_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_982</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579913.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_982"/>
    <dbReference type="EnsemblBacteria" id="ACJ75441">
        <property type="protein sequence ID" value="ACJ75441"/>
        <property type="gene ID" value="THA_982"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_982"/>
    <dbReference type="eggNOG" id="COG0446">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_1_0_0"/>
    <dbReference type="OMA" id="ACGIPYW"/>
    <dbReference type="OrthoDB" id="1149616at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="2">
        <location>
            <begin position="328"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="444" mass="49816" checksum="8E2267B550CAE5B8" modified="2009-02-10" version="1">MKVIVIGCTHAGTAFTVTAKKLYGDKVDITIYEKNDTISFLSCGIALHVGGVVKDPEKLFYSSPKQLSETGANPKMKHEVIDVNTKEKWVLVKNLETGETFKDTYDKLLISSGSWPIIPNIPGIDLEGIKLSKNFYHAQDIINHSKNAKRIAVIGAGYIGVELAEAFKENGKEVMLFDIKDRILSRYLDREFTDILEAELKNNGVELILGEKVVEFKGENNHVSKIITDKGEYNADMVVLAMGFKPNTELFKGKVEMLENGAIKVDKYLHTFENDLFAVGDSAAVWFTPTNSYEYIPLATNAVRMGTIAAYNLFDDTVEYKGTQGTSGVKVYSYNVFTTGISQQFAEERNLDVKTIFTIENNRPEFMPEYEKVYIKIVYDDKTKRILGGQIMSKANVAESINTLSLAISNNMTLVDLAFSDFFFQPHFNKPWNFLNTVALKAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH92</accession>
    <name>B7IH92_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100993.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_997"/>
    <dbReference type="EnsemblBacteria" id="ACJ75456">
        <property type="protein sequence ID" value="ACJ75456"/>
        <property type="gene ID" value="THA_997"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_997"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1757940_0_0_0"/>
    <dbReference type="OMA" id="WFTDGMK"/>
    <dbReference type="OrthoDB" id="1914312at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="148" mass="17364" checksum="8CEF757A1665A46A" modified="2009-02-10" version="1">MLKLPPMKFVEYINAPKNKVWKVFVNENGWDPWFTDGMKMEVKDGGKISFRWKRLTNGEVVTDNGYTVEIIPEKLWEFWWYEYEDGFRSKVTMKFQESVDGGTYITIIDHTLVKNTDELEIRYGCAFGWGQMLTLAKAYIEKGLILIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHH6</accession>
    <name>B7IHH6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobalt ABC transporter, permease protein CbiQ, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1084</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101164.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1084"/>
    <dbReference type="EnsemblBacteria" id="ACJ75540">
        <property type="protein sequence ID" value="ACJ75540"/>
        <property type="gene ID" value="THA_1084"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1084"/>
    <dbReference type="eggNOG" id="COG0619">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056469_5_0_0"/>
    <dbReference type="OMA" id="MFLLIYR"/>
    <dbReference type="OrthoDB" id="1479665at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="229" mass="26721" checksum="AEAE46220053F0D2" modified="2009-02-10" version="1">MIAEKISYDNRFSNIHPLPKIIFSFSLLFLTFWFSNIVNLLVIASIFLLCFLGKIKITDFLKFFLVIVLFIFLSSLTLFFDFSSLTIQRGLKVFVRSVAGTSCILFLIFTTPVVDIISFIHFRMLKDLIFLIYRAIYILLKVVEQMITAQTARFGYSNFRNTINSMKYLMYGAFSRSIKYSEESYMGLSSRNYNGTIEILKEYRLNLYILIPFVVEIVLIALEVFYVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHJ0</accession>
    <name>B7IHJ0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1098</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580000.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1098"/>
    <dbReference type="EnsemblBacteria" id="ACJ75554">
        <property type="protein sequence ID" value="ACJ75554"/>
        <property type="gene ID" value="THA_1098"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1098"/>
    <dbReference type="eggNOG" id="COG1145">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1900">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043239_0_0_0"/>
    <dbReference type="OMA" id="WVRGCYF"/>
    <dbReference type="OrthoDB" id="390505at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002708">
        <property type="entry name" value="HcyBio"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01837">
        <property type="entry name" value="HcyBio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="401"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="432" mass="48057" checksum="6ABA3EA72E01D283" modified="2009-02-10" version="1">MRSFEEINEKIEKGKVVVLTAEEAVKLSKEKGVKYVYENVDVVTTGTFSPMCSSGVFFNFGHTTPPMRMEEIKLDGVTVHAGLAAVDGFLGATQEDGIGGSYVIEKLISGQDVFLSANAKGTDCYPRKKIEGYINKDIINEFYFFNPRNVYQNYSAATNGSNKTIYTYMGKLLPNYSNVTYATSGELSPLLKDPKLKTIGIGTRIFFGGTQGYVVWQGTQYNPSTEDIPKNPSRTLAVIGNAKEMSREYIRAAKFKGYGVTIFIGIGIPIPILDEEIAYYVTRSNSELITTVRDYSKHDKPIIREVTYQELKSGKIELNGKYVKTAPITSMNVSRKIANELKKQILNKEFFLTKPVASLPSKSDYKKLNTKSTKQEKVIKNECVNCGLCTGYCNALYIENDTVKFERTNCTNCGLCSDVCPVGIELPWGLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHQ7</accession>
    <name>B7IHQ7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580054.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1170"/>
    <dbReference type="EnsemblBacteria" id="ACJ75621">
        <property type="protein sequence ID" value="ACJ75621"/>
        <property type="gene ID" value="THA_1170"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1170"/>
    <dbReference type="eggNOG" id="COG0613">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097071_0_0_0"/>
    <dbReference type="OMA" id="DDMTPNN"/>
    <dbReference type="OrthoDB" id="1309717at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="213" mass="24163" checksum="805DBD3DA3AF0367" modified="2009-02-10" version="1">MVPGVFKNSFLDVIALCDHNSGKNVRIFSNVLKEFGKVVIPGIEIQTIEDVHILGYFYDIDSLENLTDIVYTHLPKIKYDPEKFGYQLYVNEKDEFIGMEFTPLSFPTDLSLSQAVNLINKYNGIPVYAHISRKFGVLYQLGIFPNEDVKVVEVNSKDDLLIARKHGFVALSSSDAHFINQIGERYSIIEGEKSVKNILDSIIEGKVKTIWDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHR7</accession>
    <name>B7IHR7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methanol dehydrogenase regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1181</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1181"/>
    <dbReference type="EnsemblBacteria" id="ACJ75631">
        <property type="protein sequence ID" value="ACJ75631"/>
        <property type="gene ID" value="THA_1181"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1181"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034716_2_2_0"/>
    <dbReference type="OMA" id="NRFQMND"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011703">
        <property type="entry name" value="ATPase_AAA-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07726">
        <property type="entry name" value="AAA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_3" evidence="1">
        <location>
            <begin position="34"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_lid_2" evidence="2">
        <location>
            <begin position="227"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="307" mass="34519" checksum="2961CDFE617DE7FF" modified="2009-02-10" version="1">MDILEKIVKNVSVVIKGKEEKIKIIVAALFSGGHVLLEDVPGVGKTMLARALSKTFSLDFKRVQFTPDLLPSDVTGLYIFDKESGEFVFNKGPIFTNVLLADEINRATPRTQSALLEAMAESQVTVDGVTHKLYDVFFTIATQNPIESEGTFPLPEAQLDRFMVKLSLGYPDEAAEKEMLYSQLFEHPIVELGSVVSKEEFLNHLSNVKNVKVSDDIMRYIVDIVSATRKHKDIMVGSSPRGSIFLLNISRAYAYLHGRDFVLPDDVKYLAPYVLNHRIILSVEARIKKVDKYKVIEEILEEVKVVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHU3</accession>
    <name>EFG_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor G</fullName>
            <shortName evidence="1">EF-G</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fusA</name>
        <name type="ordered locus">THA_1212</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101432.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHU3"/>
    <dbReference type="SMR" id="B7IHU3"/>
    <dbReference type="STRING" id="484019.THA_1212"/>
    <dbReference type="EnsemblBacteria" id="ACJ75657">
        <property type="protein sequence ID" value="ACJ75657"/>
        <property type="gene ID" value="THA_1212"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1212"/>
    <dbReference type="eggNOG" id="COG0480">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_4_1_0"/>
    <dbReference type="OMA" id="AATTCHW"/>
    <dbReference type="OrthoDB" id="88967at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_B">
        <property type="entry name" value="EF_G_EF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000201494" description="Elongation factor G">
        <location>
            <begin position="1"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="12"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="21"/>
            <end position="28"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="85"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="139"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <sequence length="691" mass="77819" checksum="F4D9EE1D082AC199" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHW5</accession>
    <name>RL15_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplO</name>
        <name type="ordered locus">THA_1234</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101471.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHW5"/>
    <dbReference type="SMR" id="B7IHW5"/>
    <dbReference type="STRING" id="484019.THA_1234"/>
    <dbReference type="EnsemblBacteria" id="ACJ75679">
        <property type="protein sequence ID" value="ACJ75679"/>
        <property type="gene ID" value="THA_1234"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1234"/>
    <dbReference type="eggNOG" id="COG0200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055188_4_2_0"/>
    <dbReference type="OMA" id="FKNINRV"/>
    <dbReference type="OrthoDB" id="1874945at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_L15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_L15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_L15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="PTHR12934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01071">
        <property type="entry name" value="rplO_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000142891" description="50S ribosomal protein L15">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="147" mass="16057" checksum="981BB7A14967D4DA" modified="2009-02-10" version="1">MRLSDIRPTPGSMRKRIRVGRGIGSGKGKTSGKGHKGQKARGTGKVHPWFEGGQTPIHRRLPKFGFKNFTKKVYTVVNVEDLERKFNSGDEVTPEKLLEVGLIKKINDGVKILGNGEITKPLTVVAHAFSSSARRKIEAVGGKAEVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHX1</accession>
    <name>RS11_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsK</name>
        <name type="ordered locus">THA_1240</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Interacts with proteins S7 and S18. Binds to IF-3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580084.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHX1"/>
    <dbReference type="SMR" id="B7IHX1"/>
    <dbReference type="STRING" id="484019.THA_1240"/>
    <dbReference type="EnsemblBacteria" id="ACJ75685">
        <property type="protein sequence ID" value="ACJ75685"/>
        <property type="gene ID" value="THA_1240"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1240"/>
    <dbReference type="eggNOG" id="COG0100">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072439_5_0_0"/>
    <dbReference type="OMA" id="KWGVAHI"/>
    <dbReference type="OrthoDB" id="1713391at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019981">
        <property type="entry name" value="Ribosomal_S11_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_S11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_S11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="PTHR11759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03632">
        <property type="entry name" value="uS11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000141150" description="30S ribosomal protein S11">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <sequence length="129" mass="13872" checksum="32834BCD1FCFC4DB" modified="2009-02-10" version="1">MAKKTRRVVKRKKKLTVDRGVVHIKSTYNNTIITLTDPDGKVITWGSGGTAGFQGTRKGTPYAAQLAADQVAKEAVKLGMKKVDILVKGPGSGREAAIRTFQAAGLEIGVIKDVTPIPFNGCRPKKKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHZ3</accession>
    <name>B7IHZ3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative cysteinyl-tRNA synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1262</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580100.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1262"/>
    <dbReference type="EnsemblBacteria" id="ACJ75707">
        <property type="protein sequence ID" value="ACJ75707"/>
        <property type="gene ID" value="THA_1262"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1262"/>
    <dbReference type="eggNOG" id="COG2342">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058176_0_0_0"/>
    <dbReference type="OMA" id="DYRFYWK"/>
    <dbReference type="OrthoDB" id="1327691at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016063">
        <property type="entry name" value="Extracellular_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004352">
        <property type="entry name" value="GH114_TIM-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016062">
        <property type="entry name" value="TM1410-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35882:SF1">
        <property type="entry name" value="PTHR35882:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03537">
        <property type="entry name" value="Glyco_hydro_114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01545">
        <property type="entry name" value="THEMAYE10DUF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01370">
        <property type="entry name" value="TIGR01370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_114" evidence="1">
        <location>
            <begin position="53"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="303" mass="36171" checksum="1308F4BE9373CB26" modified="2009-02-10" version="1">MENFIKLLTIGILFFLIIQPLSIERAEVPLIYQLQKINLRTIYSYYKPKFLIIDVQDIDEEDIYYIKKLKENGTTILSYLSIGEAEDYRSYWKKEWNKNPPEWLGSENPMWPGNYKVKYWYIEWQNIVFNMIDEILKYEVDGLYLDLIDSFIYWSENGYPKEFTALQMIDFVFHISNYVKSKGNFLVVPQNGENILDYDTSNNYINAIDGIGIESLFFKYTKRIDEKITLNRLKYIQKIQNQGKFVLVTDYIFSPFYDNSKIINEFIELCKKHNFFGYPANKDKKLSDISGALKYFKKEKEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7II04</accession>
    <name>B7II04_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101546.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1273"/>
    <dbReference type="EnsemblBacteria" id="ACJ75718">
        <property type="protein sequence ID" value="ACJ75718"/>
        <property type="gene ID" value="THA_1273"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1273"/>
    <dbReference type="eggNOG" id="ENOG5033BXS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1659898_0_0_0"/>
    <dbReference type="OMA" id="YREILMP"/>
    <dbReference type="OrthoDB" id="1737605at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035168">
        <property type="entry name" value="DUF5317"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17248">
        <property type="entry name" value="DUF5317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="159" mass="19090" checksum="C32DAD8BD3B94C9F" modified="2009-02-10" version="1">MIVYIFLMAFLISIFTKRIKHVIERNYRYFYLFPIPFILQMIPSYREILMPLSFAFLLVLLILNKHIPGFSLISIGTILNSFVMMINNWRMPVLSYWVEKFDLPVGMRHLVVDNFSWKLFLGDWIPVILPWREYYVISIGDIFVYVGVFYFLLKINSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMV2</accession>
    <name>C0QMV2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase repeat family IS605</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_2026</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2026"/>
    <dbReference type="EnsemblBacteria" id="ACN29519">
        <property type="protein sequence ID" value="ACN29519"/>
        <property type="gene ID" value="THA_2026"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2026"/>
    <dbReference type="eggNOG" id="COG0675">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_9_1_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_IS605" evidence="6">
        <location>
            <begin position="11"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="7">
        <location>
            <begin position="122"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="35"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACN29519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="226" mass="25680" checksum="7C0D60E3D7971F15" modified="2009-05-05" version="1">MCSTTHSTIYQIGIDLGIKDTLTLSTGEKYSLPDLSKYENKLKRLHRQLSRKQKGSKNREKARLRLAKAYEKIINIKNDWIHKVTHKIVNENQVGKIVVEDININGMMKNSKLSKYIHMQSWGKFITILTYKAKNLGIEVIKADRFFQSSQTCSVCGYVNKEVKDLKIRKWVCPICKAEHDRDINAAKNLAKYGLTIPVGRGPFEFTPVDSALAEEPAKGLRAITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICJ5</accession>
    <name>B7ICJ5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580112.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1277"/>
    <dbReference type="EnsemblBacteria" id="ACJ75722">
        <property type="protein sequence ID" value="ACJ75722"/>
        <property type="gene ID" value="THA_1277"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1277"/>
    <dbReference type="eggNOG" id="COG3437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1110960_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="250" mass="29725" checksum="EF30EE46A409BBB9" modified="2009-02-10" version="1">MYLTYLLARAPYYFKDISLKELSINDITGFLRAKLNEDNITYYSVKDINDGYFENRLYIRIEDKVYLIFMKFPTSTLNSFFNNLRNLNVNFSIVKLVELYSKDGKALDGKVNDFVKERFQEIIAEEGYKRKIDRKNYEFYYPIILESAHSIFGPMVLKISFDFSVVYRTLYIFILLVVIFVIVAFFVTYRFSAKISKDISGEFDVLINNINKLKSKKELYREIPHEFKIKEIEEIASQYTAPLKDWTVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICJ9</accession>
    <name>B7ICJ9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RmuC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1281</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA recombination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RmuC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1281"/>
    <dbReference type="EnsemblBacteria" id="ACJ75726">
        <property type="protein sequence ID" value="ACJ75726"/>
        <property type="gene ID" value="THA_1281"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1281"/>
    <dbReference type="eggNOG" id="COG1322">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_727470_0_0_0"/>
    <dbReference type="OMA" id="EVEFAWD"/>
    <dbReference type="OrthoDB" id="1027804at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003798">
        <property type="entry name" value="DNA_recombination_RmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30563">
        <property type="entry name" value="PTHR30563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02646">
        <property type="entry name" value="RmuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="45"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="380" mass="44048" checksum="9604B25898138494" modified="2009-02-10" version="1">MQELIYIILTTLGLLVGVSLGYKFGYKKGKNDRNAEELFSNLSTINNLSIQIAELKAKYEEIEKARLEAEKQREKLNEEKEKRFKEFIENTQRLFSEISNQTIKIDEEKEKRINELVEQMRKFFEEQKKNTEKFLNEQGKSREEIEKRRDAQIEDMKRMISIFTKTVSGTKTRGMTGEFLLKEALKESIKVGLIKTNLKTEGGEVEFAWDLGDGKYIPIDSKFPDVFQLLEEYDKVESSKERDKLKKEIIDKVKKEIQRVQKYQNLFNTIDSCILVVPEAILEIAPELVGLGRENNVFLCSYKDVFVIAHTLQDKYIKLKEEGDIGKYKQMVSLLFQIIENINSKVSTIDKALTTIANANTFIKDYINKAKEAGSSNNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICL5</accession>
    <name>B7ICL5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mmpl family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1297</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580128.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1297"/>
    <dbReference type="EnsemblBacteria" id="ACJ75742">
        <property type="protein sequence ID" value="ACJ75742"/>
        <property type="gene ID" value="THA_1297"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1297"/>
    <dbReference type="eggNOG" id="COG1033">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008861_1_1_0"/>
    <dbReference type="OMA" id="SLMFAHI"/>
    <dbReference type="OrthoDB" id="149255at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="706"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="729"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="757"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="806"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="831"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="7">
        <location>
            <begin position="225"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="7">
        <location>
            <begin position="729"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="864" mass="97444" checksum="29A74F3CDE102855" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICL7</accession>
    <name>B7ICL7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1299</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580130.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1299"/>
    <dbReference type="EnsemblBacteria" id="ACJ75744">
        <property type="protein sequence ID" value="ACJ75744"/>
        <property type="gene ID" value="THA_1299"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1299"/>
    <dbReference type="eggNOG" id="ENOG5033S7I">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_688740_0_0_0"/>
    <dbReference type="OMA" id="FIYTISN"/>
    <dbReference type="OrthoDB" id="1255361at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="410" mass="46213" checksum="9987E9802BDF6784" modified="2009-02-10" version="1">MKKVVLALLVILASFGFSYSLLFEGGYLYYDGNDFSLETNSLTFIPVKFSTDTKFDGTFYTKSSLRFDQTFDNSYVYFDGQFFTASFFVPLAVVNVNEAYVETYTNFGDLKVGKFVEKLVDSEVFKVYDKIGSYLAGEKLSITGAKYAYTTNYGTLGLYTFSTFESLPSSKPASYVYTVKTNLPDETKMAILASMQGGGLPIVFKDQKFADHFSYAINWSGNVLGVDYGVLLAYKKSNYLVVDKILEDGVHLKWPFVYTASLSFVYQIPSTSFIVHNYTGFESENKSTFNLPVEGLGEVEIEEKVPSVLENVFGIEYQIGTQGLIGFDGYIRLYDFEYYDSAISVYGDYKKDEFGVKGMVKYNMKSEKIDVMYEGSYNVDDYTALFARGLYSKIDDSDTNVVMIGVKVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICW3</accession>
    <name>B7ICW3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1396</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580204.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1396"/>
    <dbReference type="EnsemblBacteria" id="ACJ75840">
        <property type="protein sequence ID" value="ACJ75840"/>
        <property type="gene ID" value="THA_1396"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1396"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_0_0"/>
    <dbReference type="OMA" id="FIITARV"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="233"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="400"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="445"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="4">
        <location>
            <begin position="191"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="477" mass="54307" checksum="BF17FA4BD1697C1B" modified="2009-02-10" version="1">MKTKLSIQLTVGILFIITLFFSSKIAFGLILPLLGVIITASNTKKIHHYVFLIITLVLYFSAFFNIKYLFIFSPRFNYLLSIVLYLFSLPLPGLFKFIRNRLYLMLILLIIYVTVVFFIFLAMPGDYTSKFLDPKIMKQPEMMEKIKRLFGVDDPWYVKYAKHMRNFFNLELGISFSEYPKRVEDIIAERLPRTVFLFLMTTLSSYILGYNLGKRIAWKRGGISDKVATFVGIVFWTIFTPFFMLIMIWLFGVVLGIFPLSGFIEPSKWLNVSFSTQDVFIRLLLSAFVLVAFWVVGIIVASFMKTKKAKNISVLSISLSGIILVVLYWFFNGYGYYALDIIYHVVLPVISLTILGFAGSMLVMRDTMLEIIKEDYITTAKAKGLPDKIVRDKHAARTALLPLVTSFVLSLASTVSGGVITETMFSWKGMGLTLLNATLVQDTPLAMGCLTFTGVFILVAHLVADILYAILDPRIRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICW9</accession>
    <name>B7ICW9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Spermidine/putrescine ABC transporter, periplasmic spermidine/putrescine-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101794.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1402"/>
    <dbReference type="EnsemblBacteria" id="ACJ75846">
        <property type="protein sequence ID" value="ACJ75846"/>
        <property type="gene ID" value="THA_1402"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1402"/>
    <dbReference type="eggNOG" id="COG0687">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026974_1_3_0"/>
    <dbReference type="OMA" id="SNWTEYM"/>
    <dbReference type="OrthoDB" id="1264541at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019808">
        <property type="term" value="F:polyamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015846">
        <property type="term" value="P:polyamine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001188">
        <property type="entry name" value="Sperm_putr-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019574">
        <property type="entry name" value="Periplasmic_polyamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00909">
        <property type="entry name" value="SPERMDNBNDNG"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Spermidine-binding" evidence="1">
        <location>
            <begin position="166"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR019574-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="340" mass="39172" checksum="F0D3DED0FB6F0ECE" modified="2009-02-10" version="1">MKKFWITLILIAVVVVVTFVAIPKKKVLYIYNWEEYIPEEVIKSFEEKYNCKVVYDSFASNEEMYAKIKAGGGGYDIVFPSGDHVSIMKKEGLLLKLDLSKIPNFKYLDKEILSKTIYDPNHEYSVPYMLGSTGIAVNKKYVKDYEHSWAIFEDERLKGKMTLLDDMREVFGGALKYLGYSVNTTNPKEIEEAKQLILKWKKNIVKFDASSYANGIVNGEYWVVHGYGEDIFQRVPEGEEDNFDFFIPKEGGTLWIDNMVILKDAKNVDLAYKFINHILDPKNAAIIAEFLGLPSPNTEARKYITSEPIYTLEDLKNCEFINDLGDAIELYNKAWQEIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID21</accession>
    <name>B7ID21_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA polymerase III, delta subunit superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1455</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580234.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1455"/>
    <dbReference type="EnsemblBacteria" id="ACJ75898">
        <property type="protein sequence ID" value="ACJ75898"/>
        <property type="gene ID" value="THA_1455"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1455"/>
    <dbReference type="eggNOG" id="COG1466">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_888159_0_0_0"/>
    <dbReference type="OMA" id="WIEKRFR"/>
    <dbReference type="OrthoDB" id="1453267at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="312" mass="36598" checksum="DBC91341F923CBB2" modified="2009-02-10" version="1">MPIINLSGNSELKKEEYVKELVSKLNAEYVRVYSDYTDRLEIIKEKVSNIGLFSKKTVVDVVDFEKFKASEKKEILSLLILDDVYLILRTQKKIKGISGEEFKLPNVWEEEKWKNLISEMLKEHGLEVKGEIIDFLFENVGPDEFALYNEIKKLKVFEKNLNLDILKDVIHKYTLSKLDDFCFAISEMKEESFRLLKDVVNDYEPIIIVYTLSSHFISLFRIFAYTKKKTSFSWPEISKLSKELKIPSPKVARFLGFKFKGQKFEPVNHLLIYSEEKLKDIIKRLYFIDRSVKSGGTTEVEILNLIKFIKEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID46</accession>
    <name>B7ID46_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101961.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1480"/>
    <dbReference type="EnsemblBacteria" id="ACJ75923">
        <property type="protein sequence ID" value="ACJ75923"/>
        <property type="gene ID" value="THA_1480"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1480"/>
    <dbReference type="eggNOG" id="COG1237">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036012_1_0_0"/>
    <dbReference type="OMA" id="CSGWRAK"/>
    <dbReference type="OrthoDB" id="1815576at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07713">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041712">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="5"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="218" mass="25347" checksum="74186496780A6724" modified="2009-02-10" version="1">MFDTGQTDVFIKNGEKLEIDFSKVEKVIISHGHYDHIGGLIFLQNVANPEVFIHKKALVERYSASKFAGVPYDWNKIELKVNFIDSDISTDEVRIITNIKMREDIIDEKFRLSDGRKDFFEDEINIVIENFLFTGCAHRGIENIVEQVIKRYNIEYVVGGFHLVNASNERIRKITELFEKYDLKVIPLHCTGENAIKFMKDRLKDKCIILNSGDEWGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID48</accession>
    <name>B7ID48_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane-associated phospholipid phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1482</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580254.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1482"/>
    <dbReference type="EnsemblBacteria" id="ACJ75925">
        <property type="protein sequence ID" value="ACJ75925"/>
        <property type="gene ID" value="THA_1482"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1482"/>
    <dbReference type="eggNOG" id="COG0671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1233510_0_0_0"/>
    <dbReference type="OMA" id="FCFETIN"/>
    <dbReference type="OrthoDB" id="1930882at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857297" evidence="1">
        <location>
            <begin position="22"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="93"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="207" mass="24060" checksum="7925372F8BB4641E" modified="2009-02-10" version="1">MKKLFLFVLLFFQVFSFSFNILDETQKDISALQFDIKEPLLFSTTFLVDGKVKRLELSKFDNFCFETINNLDEKDFLILTALAAGITYFDDPALSYTILESTFFTSVVTYAMKISIGRGRPYAYDSPFVFKPFSFEDKNFSFPSGHSSFAWALFTPLAERYNKGLYIFPILFSISRVVGNYHWLSDVIFGAFIGYTISKCFYYIYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID50</accession>
    <name>B7ID50_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Periplasmic copper-binding protein NosD, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101969.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1486"/>
    <dbReference type="EnsemblBacteria" id="ACJ75927">
        <property type="protein sequence ID" value="ACJ75927"/>
        <property type="gene ID" value="THA_1486"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1486"/>
    <dbReference type="eggNOG" id="COG4677">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_927288_0_0_0"/>
    <dbReference type="OMA" id="VANNIEM"/>
    <dbReference type="OrthoDB" id="1177619at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="300" mass="33597" checksum="03FFACCB9C66FD40" modified="2009-02-10" version="1">MKKSLIFIFLLVFSIILVSETIIVEENIQQAIDKAKDGDIIYLKAGTYKENLSIVSKNLTLVGEEGAYISYVGKDPKNIFNSSPTIYIENSNVIIKNLEIGSVNSIGIGLLNSKISLSNVNMNLDNAYGVVCVADEEKESFLEVSNLSVEDVNYKSSSGKNVSLNFNSVGIMLSGDSMFVANNIEMKGMKNGLILNNKMSYIYNSDFLENENVAIILSGQQKFINNRFELNVETFKVANSPEVELVGNVFKDNSYNFVIKKDDYDSYQNFRHFSGKIIGFSNILQSPEILNESFGKYFWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID59</accession>
    <name>B7ID59_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">L-threonine 3-dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1495</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101995.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1495"/>
    <dbReference type="EnsemblBacteria" id="ACJ75936">
        <property type="protein sequence ID" value="ACJ75936"/>
        <property type="gene ID" value="THA_1495"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1495"/>
    <dbReference type="eggNOG" id="COG1063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_0_0"/>
    <dbReference type="OMA" id="ETWYAMS"/>
    <dbReference type="OrthoDB" id="972769at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="3">
        <location>
            <begin position="10"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="343" mass="37438" checksum="221ECEEC33748A02" modified="2009-02-10" version="1">MKAILKETAGPGFVMKEVPKPEKLGPREVLVKIRRASICGTDVHIYKWDEWSQSRINPPLIVGHEMAGEVVAIGEAVTQVKVGDLVSAETHIPCEHCVQCKTGRMHVCKNLEILGVDRNGVFTEYAIIPETVLWRFSKEIPLDYASVMEPFGNAIHTALVTDLTGKKVLITGAGPIGLMAIQVAKASGASLVITSEVDPNRIQMAKENGADIVINPAEQDLVKSIYTITDDGVDILLEMSGNKKALEDGLKCVTMGGEASLLGIFGGSIDINLDSLVIMRGITIYGITGRKMFDTWKVADELLRSKKVDLSKVVTHVLPFEDWEKGFELMLNKQCGKVVLNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID65</accession>
    <name>B7ID65_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1501</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102006.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1501"/>
    <dbReference type="EnsemblBacteria" id="ACJ75942">
        <property type="protein sequence ID" value="ACJ75942"/>
        <property type="gene ID" value="THA_1501"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1501"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_0"/>
    <dbReference type="OMA" id="IEGHVQY"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="269" mass="31011" checksum="3850AF25B8619498" modified="2009-02-10" version="1">MLKIENLKKSFKNKFVLNNVSLEVEEGEILALIGPNGFGKTTTLNCISFVIKKDGGKIILDDQEFTEEEKYKISYVPEDRVFFQNLTGKDYQKIWSSIYPEWNENVFQKFITKYNLDLNQKVEKYSQGMKTLFLVGLSVSTNADILLLDEPTQHLDPTIRLEIMKIIRNYADLGKYVIISSHEIFEIEEYADKIAIIKSGEVIYKDYIDDAKEKHKIIDSEEKITSGEVIGLVGDKILIKTDEEIGRYPKLNEIVVGYLNSKNKVDIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDC3</accession>
    <name>B7IDC3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Penicillin-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1562</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580276.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1562"/>
    <dbReference type="EnsemblBacteria" id="ACJ76000">
        <property type="protein sequence ID" value="ACJ76000"/>
        <property type="gene ID" value="THA_1562"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1562"/>
    <dbReference type="eggNOG" id="COG0768">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009289_1_2_0"/>
    <dbReference type="OMA" id="TPYAWFI"/>
    <dbReference type="OrthoDB" id="423699at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005311">
        <property type="entry name" value="PBP_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03717">
        <property type="entry name" value="PBP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_dimer" evidence="4">
        <location>
            <begin position="127"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="3">
        <location>
            <begin position="208"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="538" mass="60945" checksum="31DA205CA38F1FF8" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDC5</accession>
    <name>B7IDC5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1564</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1564"/>
    <dbReference type="EnsemblBacteria" id="ACJ76002">
        <property type="protein sequence ID" value="ACJ76002"/>
        <property type="gene ID" value="THA_1564"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1564"/>
    <dbReference type="eggNOG" id="ENOG502ZRVQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1593773_0_0_0"/>
    <dbReference type="OrthoDB" id="1968843at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="169" mass="20207" checksum="B3137244C3AB6E91" modified="2009-02-10" version="1">MFILKKKKKDKLNFSIAFVKNFENLKTIIKSIKKQKIDEVFFIIDRNIEKDHLKTIKKIIKANFKNYSILSKNFQKFSKNVAKVERLNVFELRRLQNKKKILYSQQSILSWKIPQMFPFYTIAFEDNTLYFCASIPLTKDSSGFLLRKKMVSDFIFNITLDLKILDELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDE8</accession>
    <name>B7IDE8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitric oxide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1587</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the zinc metallo-hydrolase group 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102332.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1587"/>
    <dbReference type="EnsemblBacteria" id="ACJ76025">
        <property type="protein sequence ID" value="ACJ76025"/>
        <property type="gene ID" value="THA_1587"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1587"/>
    <dbReference type="eggNOG" id="COG0426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017490_0_0_0"/>
    <dbReference type="OMA" id="HVKNNIH"/>
    <dbReference type="OrthoDB" id="1149616at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016440">
        <property type="entry name" value="Rubredoxin-O_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005243">
        <property type="entry name" value="ROO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="2">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="388" mass="44107" checksum="7CC2DB21A8FE3ADC" modified="2009-02-10" version="1">MVRKIAENVYFVGAIDWDRELFDELIPLPNGTTYNSYIIKGTEKIALIDTVEPRKEEEFMNNLKKLNIQKIDYVISNHAEQDHSGLIGKVVEKFGAKVVTNKKCADFLKTHIHIKDEDVITIEDGQEISLGDKTLKFIFTPWTHWPETMVTYLKEDKILFSCDLFGSHYATSEIEQVGSEKLLEMAKRYYAEIMMPFRVPMKKNIEKVEMLDIEKIAPSHGPVYKNPKLIIEAYKKWISDEVENEVVILYVSMHESTRKMVEYLVESLMDKGVKVKLHNIVNADIGEIAIDLVEASTVVIGTPMVLTGVHPIVAGAIYLVNALRPKTKYLAIVGSYGWGGKFIDEIKSMLSNVKAELLEPVTVKGLIDSEGYEKLEHLAKEIAERNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDH9</accession>
    <name>B7IDH9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580299.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1618"/>
    <dbReference type="EnsemblBacteria" id="ACJ76056">
        <property type="protein sequence ID" value="ACJ76056"/>
        <property type="gene ID" value="THA_1618"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1618"/>
    <dbReference type="eggNOG" id="ENOG503311K">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_727471_0_0_0"/>
    <dbReference type="OMA" id="IRRRANF"/>
    <dbReference type="OrthoDB" id="1341558at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="380" mass="44349" checksum="1446379DDF456137" modified="2009-02-10" version="1">MKSENYRKKIEEKERKLKEEIQSYRRRSVYILFFNVFLVVVLFFLFNSVKGKDTTIVEGYQIAIKMPSEIYSDEEVQSKVFLINTRNYKRDFVIDRFKFQILKENVPVYNFYYDSPISSTIDRLESILVFDLSREVSLKNLKSGIYIVSVEMYLDGKLIKNSFNFSVKEEILKEIAVDPYYSIGENIIPQLVIENKTATTVVLSPTEIEWTLNNKTYTDVFSQEYSLKPGEKMFFESSNIFKINKKGSYNLKCTIYFKNGIETISKNINVIEVPEKSLKELDLNIYSDEYVVSGRKVNFNFEILNRSEKERYLYFNKVVIIIPKINYSYEVSNVKVLLGRYGGMSLVTLPLVFPAPGKYDIVFKLDAGETISKVLTLQVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDK9</accession>
    <name>B7IDK9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dehydrase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102452.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1648"/>
    <dbReference type="EnsemblBacteria" id="ACJ76086">
        <property type="protein sequence ID" value="ACJ76086"/>
        <property type="gene ID" value="THA_1648"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1648"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056342_5_2_0"/>
    <dbReference type="OMA" id="DHIAVWI"/>
    <dbReference type="OrthoDB" id="775108at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="17"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="248" mass="28051" checksum="678C2409C0C59C70" modified="2009-02-10" version="1">MIKNINNKVYIIENELASNSTLIIGKKGNILIDTSLFPKKAEKIKEFSKDISGKEITLVINTHYHPDHTFGNSAFDCPIVGHSLTRNLMEQIDEKYLMQLELEDIKIVPPNIIFDNFFEYEDGVKLTVYHGPGHTPDSSYIIIENEDILIAGDTVITDIHPEIVSDSNLDIWINTLENLPQVSNIVPGHGKIGNFADVGLMKEYLQKIKNLKNGTVSSTSLENDPNFTNRKYPELLKWSLENLLKNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDN3</accession>
    <name>B7IDN3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580341.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1672"/>
    <dbReference type="EnsemblBacteria" id="ACJ76110">
        <property type="protein sequence ID" value="ACJ76110"/>
        <property type="gene ID" value="THA_1672"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1672"/>
    <dbReference type="eggNOG" id="ENOG503480V">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1659885_0_0_0"/>
    <dbReference type="OMA" id="FSLYQIR"/>
    <dbReference type="OrthoDB" id="1826264at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="159" mass="18194" checksum="233204C4AD89AA20" modified="2009-02-10" version="1">MLDVTFFTLITGAVGLLISIALAFLIVISKTFFKYGTFRFFNELIVLGLGILGWYYIPKGILQLFFFLILSTLYQLYRIIRDIYSIDTRFRILVLALGKDRFEYAIFSIKRVSKRIIGSFFKYFAILIAAYPISQSLHAEIVGVISLLVGVILVLLKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDT9</accession>
    <name>B7IDT9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enzyme of the cupin superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102608.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1731"/>
    <dbReference type="EnsemblBacteria" id="ACJ76166">
        <property type="protein sequence ID" value="ACJ76166"/>
        <property type="gene ID" value="THA_1731"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1731"/>
    <dbReference type="eggNOG" id="COG3450">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135880_2_0_0"/>
    <dbReference type="OMA" id="CPPGKYM"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008579">
        <property type="entry name" value="UGlyAH_Cupin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05899">
        <property type="entry name" value="Cupin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_3" evidence="1">
        <location>
            <begin position="13"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="89" mass="10666" checksum="840A1906E0D3FC63" modified="2009-02-10" version="1">MEIKITTPSREELEKLGVFSWPIWTKEVSEFDWYYDETEVCYILEGEIEVETKDGKVYKIKPGDLVEFPKGLSCRWKVKKAVRKHYNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDW6</accession>
    <name>B7IDW6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1762</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580393.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1762"/>
    <dbReference type="EnsemblBacteria" id="ACJ76193">
        <property type="protein sequence ID" value="ACJ76193"/>
        <property type="gene ID" value="THA_1762"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1762"/>
    <dbReference type="eggNOG" id="COG4254">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_701946_0_0_0"/>
    <dbReference type="OMA" id="YEFGPEF"/>
    <dbReference type="OrthoDB" id="102343at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="395" mass="44061" checksum="2FBB20F18EC5E269" modified="2009-02-10" version="1">MKIKKLLVIFVLVLSLIAVSQTYINIGTIERNNEKFFVYELSPEFSIGPVTIGIGFTSYSTDVVYGEMYYGIPSSTPSTNILNAFVINTFALNTDLFEFRYGKVKPITLAMGFNVRKYVNPNTRAFDITLKFSDLSLYTHLPYEILKYIPFEFSRSDSIFMTSLGYNLSSLLKIQSYLIADVDATVSYTEDSSTPVKYGGGIAAYIPILNSIKIGGEIAAQSDESFQKISKGLFAGVFADLGLINPMGGIYYTMDGYIPFLFNKKYSLLKFNSNLPSMSSTNNTLGYFAGADIDFEPYITGEFYVYGLLENSTPVAEGNVRVILPELGNFSGLIIDGTYFDDSPFEDGKLLDENTEAILRLSYPLIGNNFVAGIQYKWESDEWFKSIFISSVSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDW9</accession>
    <name>B7IDW9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MazG protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102664.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1765"/>
    <dbReference type="EnsemblBacteria" id="ACJ76196">
        <property type="protein sequence ID" value="ACJ76196"/>
        <property type="gene ID" value="THA_1765"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1765"/>
    <dbReference type="eggNOG" id="COG3956">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038356_0_1_0"/>
    <dbReference type="OMA" id="HPHIYGD"/>
    <dbReference type="OrthoDB" id="1099129at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004518">
        <property type="entry name" value="MazG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011551">
        <property type="entry name" value="NTP_PyrPHydrolase_MazG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30522">
        <property type="entry name" value="PTHR30522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03819">
        <property type="entry name" value="MazG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00444">
        <property type="entry name" value="mazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MazG" evidence="1">
        <location>
            <begin position="30"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="MazG" evidence="1">
        <location>
            <begin position="167"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="251" mass="29382" checksum="7C1B7FA221CD53F6" modified="2009-02-10" version="1">MATTGEKFEKLINIMKQLRGPNGCDWDKKQTHDSLIPYLIEEAYELIEEIKNKNYDNLKEELGDILLQVIFHAQIASEEGKFSIDDVIDTISDKLIRRHPHVFGNEQDFSYIRWEKIKAQEKGENDFSRIGKVNYSLPALSLSRRIQENAANVGFDWDNIEDVYSKVYEEIDELKNAKNGEEAEDELGDLLFAIVNLSRFLKIDPEVALRKATEKFVKRFKKMEKLIEKDGKKFEDLPLDELNKYWEESKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDX2</accession>
    <name>B7IDX2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc-dependent proteinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580396.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1768"/>
    <dbReference type="EnsemblBacteria" id="ACJ76199">
        <property type="protein sequence ID" value="ACJ76199"/>
        <property type="gene ID" value="THA_1768"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1768"/>
    <dbReference type="eggNOG" id="COG2738">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084406_0_0_0"/>
    <dbReference type="OMA" id="VQHATAY"/>
    <dbReference type="OrthoDB" id="1656873at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007395">
        <property type="entry name" value="Zn_peptidase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36434">
        <property type="entry name" value="PTHR36434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04298">
        <property type="entry name" value="Zn_peptidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="230" mass="25302" checksum="44923CF99AE3D8BD" modified="2009-02-10" version="1">MFFFDPTFIILIPGLLLAFIAQAYVQERFSRYSKIPSSLNMTGAQLAKYMLESAGLYNVKIEKIPGHLTDHYDPRTKVVRLSDATYNSNSIAALGVVAHEIGHAIQHARHYVPLVIRTGIAPVVSFASNLSWILFIAGFIFMNIALIKIGVLLFSLAVIFSLITLPVEFDASNRAVKILRTNLMMPPSELEGVKKVLGAAAMTYVAGTLMAFLQLLRMLLIAGFLGGNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDZ7</accession>
    <name>B7IDZ7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase ((3R)-hydroxymyristoyl ACP dehydrase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1793</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580418.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1793"/>
    <dbReference type="EnsemblBacteria" id="ACJ76224">
        <property type="protein sequence ID" value="ACJ76224"/>
        <property type="gene ID" value="THA_1793"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1793"/>
    <dbReference type="eggNOG" id="COG0764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078912_1_2_0"/>
    <dbReference type="OMA" id="FPGRPLM"/>
    <dbReference type="OrthoDB" id="1763197at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013114">
        <property type="entry name" value="FabA_FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272">
        <property type="entry name" value="PTHR30272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07977">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="136" mass="15368" checksum="3CF3E3884C387161" modified="2009-02-10" version="1">MKKEEIMKILPHRDPILLVDRVVEKGEDYIVAEREIKDSDPIFKGHFPNYHIYPGVYILEGLAQTAGILLLKGNDEIPLFLGIDNARFKGEVRPDCTLRYEVKIKEIKAGVVFVDAKAYVSSKMVAKAVLLLGSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE25</accession>
    <name>B7IE25_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580444.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1821"/>
    <dbReference type="EnsemblBacteria" id="ACJ76252">
        <property type="protein sequence ID" value="ACJ76252"/>
        <property type="gene ID" value="THA_1821"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1821"/>
    <dbReference type="eggNOG" id="COG2206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_92_1_0"/>
    <dbReference type="OMA" id="TIIGTHM"/>
    <dbReference type="OrthoDB" id="1094814at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD-GYP" evidence="1">
        <location>
            <begin position="106"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="331" mass="37621" checksum="F47D0C3F0B35D5FB" modified="2009-02-10" version="1">MIIWKNVNKVKPGEKLGEDVYDPSGFIILLKKGSILKEGDIVLLKNRGVNSLPIYEEEIFISSEVEPIIDYENYRTLSEELERAFNEIKEGEINISAMVEQSENILNEVLKKIDKKILNLLNNDSKPLIRHCINVAVISSIIGINLGFDRELLTKLVISSFLHDLSHDKPIRDVINYYDTHPIKSAALIKNYFETNNDILLSVLHHHERYDGKGFPRGLRESSIPIFSRIIAVADAYDTLISKDFPGDALTPYEALKFIIINSGKYFDPQIVSKFVHYVGIYPTGTIVELSDGRKGVVLKVSNGVFPIVKVENREIDIKKEKISIKRILKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE55</accession>
    <name>B7IE55_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hypothetical cytosolic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1856</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102831.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1856"/>
    <dbReference type="EnsemblBacteria" id="ACJ76282">
        <property type="protein sequence ID" value="ACJ76282"/>
        <property type="gene ID" value="THA_1856"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1856"/>
    <dbReference type="eggNOG" id="COG0727">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1728666_0_0_0"/>
    <dbReference type="OMA" id="GECTFLT"/>
    <dbReference type="OrthoDB" id="1517287at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="147" mass="16794" checksum="2004D9D0AA9FD172" modified="2009-02-10" version="1">MFINEKICKKCGGKCCKSYPGIALPKDFGNSKEEILQKLTQALKSGKWTIDWNDRKKEEYFVRPAVKGKENFIFDHSISGECVFLTKSGCKLKYNERPSGCLLLEPKSNEKCIQHLSKRLAIEDWSKYKDIIIEAAINAEEIEGDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE65</accession>
    <name>B7IE65_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1866</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580464.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1866"/>
    <dbReference type="EnsemblBacteria" id="ACJ76292">
        <property type="protein sequence ID" value="ACJ76292"/>
        <property type="gene ID" value="THA_1866"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1866"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_645206_0_0_0"/>
    <dbReference type="OMA" id="IECGQRH"/>
    <dbReference type="OrthoDB" id="1108073at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001119">
        <property type="entry name" value="SLH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00395">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="25"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="329"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="422" mass="48247" checksum="67593A827643832C" modified="2009-02-10" version="1">MRKVLSIFLIMFLMMIIFAVETVITDLSPIVPEYSAVKFLVENQIMDLDVNGNFKPSLLITRLDIAKVIYTVIQKYNLSKISDIEKSLANISDEIKIAKGLVSGIDKRLVNLETKQEELTQKIEAFDAFLTQSSETLFANIEKNYVKREDFDSLKAQITLISQVYNSQTKKFEEKINDIEKISEIEKKINIVSNQLEDLKTNFLEEQAKNIAKFSDIEKQLSNIPTNVTNLNVTVAQLSNKVSSIEELYTKLSNIKASDIDKLENLSELEDKVNQMYSILTDLNAFSMDLDSLRKRLEGIDILTVRNVVNKFSILENRYQQIDSRVQYLENSVGKIELLNQKIEELNAKISDIENKKIMLDNLEKISNDVEELNKYKEDSKKALDIMSDKLVETNNNMRTILIVSIVSAAISVISFILTITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE72</accession>
    <name>B7IE72_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Yggt family, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1873</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YggT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102871.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1873"/>
    <dbReference type="EnsemblBacteria" id="ACJ76299">
        <property type="protein sequence ID" value="ACJ76299"/>
        <property type="gene ID" value="THA_1873"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1873"/>
    <dbReference type="eggNOG" id="COG0762">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136788_1_0_0"/>
    <dbReference type="OMA" id="VRPDPYN"/>
    <dbReference type="OrthoDB" id="2069856at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003425">
        <property type="entry name" value="CCB3/YggT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02325">
        <property type="entry name" value="YGGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="99" mass="11541" checksum="34E0A4163E28603A" modified="2009-02-10" version="1">MFILGNFLIGLGYSLRVLINIEMFFIVISAVLSWFSYYSNVQSIYYYFQSIADIIEKPIRKLIPRIGPVDISPMVAIVVLVFFDRFLIQSIIELGYYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE77</accession>
    <name>B7IE77_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bifunctional purine biosynthesis protein PurH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102877.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1878"/>
    <dbReference type="EnsemblBacteria" id="ACJ76304">
        <property type="protein sequence ID" value="ACJ76304"/>
        <property type="gene ID" value="THA_1878"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1878"/>
    <dbReference type="eggNOG" id="COG0138">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016316_2_1_0"/>
    <dbReference type="OMA" id="CVNLYNF"/>
    <dbReference type="OrthoDB" id="1194473at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="PTHR11692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MGS-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="201" mass="22592" checksum="F0CBD100D04680A0" modified="2009-02-10" version="1">MNVKRALISVYNKEGIVEFAKKLSEWGVEIISTGNTANLLEENGIPVIRVSEVTGFPEILGGRVKTLHPKIFGGILAKFGDSKHMKDLSDNNIKPIDMVVVNLYPFELVAKETRNEEKLIENIDIGGVALLRAAAKNYRDVIVISDPEDYDKVLKSLEECGDVILHQRRILALKAFYRTMKYDAEIHSVLSELFASNNFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE87</accession>
    <name>B7IE87_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="5">SRP receptor</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">ftsY</name>
        <name evidence="7" type="ordered locus">THA_1888</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102891.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1888"/>
    <dbReference type="EnsemblBacteria" id="ACJ76314">
        <property type="protein sequence ID" value="ACJ76314"/>
        <property type="gene ID" value="THA_1888"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1888"/>
    <dbReference type="eggNOG" id="COG0552">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_3_0_0"/>
    <dbReference type="OMA" id="GISDQFQ"/>
    <dbReference type="OrthoDB" id="656414at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031226">
        <property type="term" value="C:intrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF7">
        <property type="entry name" value="PTHR43134:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54" evidence="6">
        <location>
            <begin position="266"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="99"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="181"/>
            <end position="185"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="245"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="294" mass="32242" checksum="64A5F903EFF49B7F" modified="2009-02-10" version="1">MGFFEKFKQGLKKTRESFFGKMKSILSGKKLDDETKEEIEELLILSDVGFEATEYILNRIEELKGEDAYESLKKVLLEILNFNSELKIEKKPFVISMVGVNGSGKTTTAGKLAEYFSKSGKSVVLAACDTFRAAAIDQLKVWAERTSSTFIAHQEGADSAAVAFDAVSHAISKEKDIVILDTAGRLHTKKNLMEELRKINRVIKKKIEDAPHEVLLVIDAVTGQNGLQQAKIFKEFVDITGIVLTKLDGTAKGGIAIAIAKELGIPIKFVGLGEGVDDLKPFDAEDFVNALLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE95</accession>
    <name>B7IE95_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102906.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1896"/>
    <dbReference type="EnsemblBacteria" id="ACJ76322">
        <property type="protein sequence ID" value="ACJ76322"/>
        <property type="gene ID" value="THA_1896"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1896"/>
    <dbReference type="eggNOG" id="ENOG50330NA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1179762_0_0_0"/>
    <dbReference type="OMA" id="QISGAYH"/>
    <dbReference type="OrthoDB" id="1807750at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="SSF69318"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002854663" evidence="1">
        <location>
            <begin position="20"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="236" mass="27120" checksum="5E1997FB9959F602" modified="2009-02-10" version="1">MKKIVLSIILFIFSIFLHANVITSTNTDIDNDGILENITLEGNKLSEIYYDAFVLKIKNEKSEKNIKLDFGGYSPKMEIYDFDGDSIKDILIISDSGGSGNYSEYFIYSIKKGVLLNNDLKLDLKAKFFDGFKALISYKDKYTFLDLSEKKEQYINEKIYNKFGQFIGENKDLFIGGISNLEAFDFGKDDIYELKGIITLSGIYHADRIGYIHFVYSIKNDKFIWLEVSKLLYAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEB4</accession>
    <name>B7IEB4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Permeases of the drug/metabolite transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1917</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102936.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1917"/>
    <dbReference type="EnsemblBacteria" id="ACJ76341">
        <property type="protein sequence ID" value="ACJ76341"/>
        <property type="gene ID" value="THA_1917"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1917"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_4_0_0"/>
    <dbReference type="OMA" id="LWMTGLK"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="134"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="278" mass="31792" checksum="668AC96392A9530F" modified="2009-02-10" version="1">MSVIFGLSFLFTKNALDFTTPYNFLSIRFLFASLSFLILFALKIIKIKNTKKLYKLLIVAFFQPILYFIFETNGLKFASSSEAGILIATIPIFITFLSPFILKEKVPKINYLFVFLSFSGVLLIMGLNVNEGFKGKILILFAVFSAVFYNFASKYFSKEFSPQEVTFVMMITGWIFFTILALINRELNYNVISNKNVFISAIYLGIFSSTIAFFLINYMLSKVSPVQSSIFSNLTTVVSVIAGFFIRHERITFNHIIGMSLIILGVWGVNYFKYRSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEC4</accession>
    <name>B7IEC4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1928</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580487.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1928"/>
    <dbReference type="EnsemblBacteria" id="ACJ76351">
        <property type="protein sequence ID" value="ACJ76351"/>
        <property type="gene ID" value="THA_1928"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1928"/>
    <dbReference type="HOGENOM" id="CLU_1331394_0_0_0"/>
    <dbReference type="OrthoDB" id="2143091at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="206" mass="24095" checksum="4E87A50AAA221892" modified="2009-02-10" version="1">MKKILVFLLLVFTIFLFSSTIDYVLVVPSDVLVSEITSLIYSLESYDFKISLTDYGLSKKNYEYLYSPYDEYFKNHILTLLSYFSNRDVNSDYILPLDFISEYQVQGDLYLYSVSLFDSDLNLLYNAFNKIAYDSNNSINSEALTIYSYLSSLLNSMFEDDSPKNYDIDYVVASVYIDTHDEGYLVFEIGFISQNNRKIDLIYYTF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEE2</accession>
    <name>PCP_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyrrolidone-carboxylate peptidase</fullName>
            <ecNumber evidence="1">3.4.19.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-oxoprolyl-peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pyroglutamyl-peptidase I</fullName>
            <shortName evidence="1">PGP-I</shortName>
            <shortName evidence="1">Pyrase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pcp</name>
        <name type="ordered locus">THA_1947</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes 5-oxoproline from various penultimate amino acid residues except L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.</text>
            <dbReference type="EC" id="3.4.19.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C15 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.3" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580495.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IEE2"/>
    <dbReference type="SMR" id="B7IEE2"/>
    <dbReference type="STRING" id="484019.THA_1947"/>
    <dbReference type="MEROPS" id="C15.001"/>
    <dbReference type="EnsemblBacteria" id="ACJ76369">
        <property type="protein sequence ID" value="ACJ76369"/>
        <property type="gene ID" value="THA_1947"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1947"/>
    <dbReference type="eggNOG" id="COG2039">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043960_4_0_0"/>
    <dbReference type="OMA" id="HHIATRA"/>
    <dbReference type="OrthoDB" id="1927224at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016920">
        <property type="term" value="F:pyroglutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00501">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00417">
        <property type="entry name" value="Pyrrolid_peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000816">
        <property type="entry name" value="Peptidase_C15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029762">
        <property type="entry name" value="PGP-I_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033694">
        <property type="entry name" value="PGPEP1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033693">
        <property type="entry name" value="PGPEP1_Glu_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PTHR23402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01470">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015592">
        <property type="entry name" value="Prld-crbxl_pptds"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00706">
        <property type="entry name" value="PYROGLUPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="SSF53182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00504">
        <property type="entry name" value="pyro_pdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01334">
        <property type="entry name" value="PYRASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01333">
        <property type="entry name" value="PYRASE_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0788">Thiol protease</keyword>
    <feature type="chain" id="PRO_1000124004" description="Pyrrolidone-carboxylate peptidase">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00417"/>
        </source>
    </evidence>
    <sequence length="205" mass="22818" checksum="3D6E8BC7E2F10FD2" modified="2009-02-10" version="1">MKILITGFEPFGGEVVNPSFEAVKHLPDSIEKAQIVKAAIHTVFRKSIEVLEELIVKEKPDIVICVGQAGGRAEITIERVAINIDDAKNPDNEGNTPKDEVIFEDGENAYFSNLPIKKMVEEIKNCKIPASISNSAGTYVCNHLMYGLLYLINKKYKNMKGGFIHVPYLPQQVLNKKNVPSMSLDNIVQALVCSIKAILKEYNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEF6</accession>
    <name>B7IEF6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR-associated endoribonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1961</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580509.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1961"/>
    <dbReference type="EnsemblBacteria" id="ACJ76383">
        <property type="protein sequence ID" value="ACJ76383"/>
        <property type="gene ID" value="THA_1961"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1961"/>
    <dbReference type="eggNOG" id="COG1583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089858_2_0_0"/>
    <dbReference type="OMA" id="HWDLYPH"/>
    <dbReference type="OrthoDB" id="908607at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045747">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36984">
        <property type="entry name" value="PTHR36984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01881">
        <property type="entry name" value="Cas_Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005054">
        <property type="entry name" value="PF1131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005054-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="244" mass="28529" checksum="7A92FF47A9469940" modified="2009-02-10" version="1">MRLKLEFISDEDIILPIGFNKYIQALIYNLFSSKLKKFVHEDGFRGKKKFSLFCFSSILERGKFFKNMKIFNFGKKISFFISSPVDMLMEDLIINLFKGDEFMLGENKIYLSSAMAVIKKVSESSIKVKAITPIEVHRTYKENGKNKTRYYTPFEKEFAELINLNIKNKWESFYKEKLDKDIEIVPLFDDERYKSVVYYGFGKKRFVIEGWKGSYLLKGDKEILSFAYDAGLGSKNSQGFGFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEG3</accession>
    <name>B7IEG3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1969</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 130 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103038.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1969"/>
    <dbReference type="EnsemblBacteria" id="ACJ76390">
        <property type="protein sequence ID" value="ACJ76390"/>
        <property type="gene ID" value="THA_1969"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1969"/>
    <dbReference type="eggNOG" id="COG2152">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046648_0_0_0"/>
    <dbReference type="OMA" id="KYYMIWG"/>
    <dbReference type="OrthoDB" id="1622507at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007184">
        <property type="entry name" value="Mannoside_phosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34106">
        <property type="entry name" value="PTHR34106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04041">
        <property type="entry name" value="Glyco_hydro_130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016202">
        <property type="entry name" value="PH1107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="294" mass="34207" checksum="57A8E6C29446A191" modified="2009-02-10" version="1">MELKLERHPRNPLFAPNPNHLWESRFVFNPAVVYDGELFHMLYRAQGEDMVSRMGYAVSIDGVNWNRFEKPVFSPATQEELYGVEDPRITYLDGYYYVNYTAYSPTGIKVAMARTKNFITYERFGPILPESPNKDAALFPEKINGKYVLIHRIEPDIWLAFSDDLIHWGDYVKIASPRKDYWDNVKIGAGAPPIKTEYGWLLLYHGVQEGPRYTYRLGFIVLDLNDPTKVIKRSETPILEPEEEWEIFGGVPNVVFSDAMVRYKDKYYVYYGAADNYIALATIEIEKVEKWIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEG8</accession>
    <name>B7IEG8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Arsenical-resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">arsB</name>
        <name evidence="5" type="ordered locus">THA_1974</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the arsenical resistance-3 (ACR3) (TC 2.A.59) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580519.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1974"/>
    <dbReference type="EnsemblBacteria" id="ACJ76395">
        <property type="protein sequence ID" value="ACJ76395"/>
        <property type="gene ID" value="THA_1974"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1974"/>
    <dbReference type="eggNOG" id="COG0798">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022869_0_2_0"/>
    <dbReference type="OMA" id="CTAMVLM"/>
    <dbReference type="OrthoDB" id="1493959at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015103">
        <property type="term" value="F:inorganic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004706">
        <property type="entry name" value="Arsenical-R_Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43057">
        <property type="entry name" value="PTHR43057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005508">
        <property type="entry name" value="Acr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00832">
        <property type="entry name" value="acr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="283"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR005508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="345" mass="38605" checksum="48BA8426F24987D1" modified="2009-02-10" version="1">MKKKMKFFEKYLSIWVALCIIGGFLLGKIFPQVPKTLSKWEYANVSIPIAILIWLMIYPMMLKIDFSSIKNVGRNPKGLIITLTVNWLIKPFTMYFIAYFFLKIVFGNIIGVELSSEYVAGAVLLGAAPCTAMVFVWSYLTNGDALYTLVQVAINDLVILFAFAPIVSFLLKVGNITVPFKTLIFSVVIFVLIPLIVAIITRHYVIKNKGKEYLERKFIPKFSTISIIGLLLTLILLFSFQGETIEKNFIHILLISIPLIIQTFLIFTIGYFWAYFWKVPHSIAAPAAMIGASNFFELAVAVAISLFGMNSGAALATVVGVLVEVPVMLTLVKIANTTRKKFKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEH9</accession>
    <name>B7IEH9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Extracellular solute-binding protein, family 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1985</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580529.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1985"/>
    <dbReference type="EnsemblBacteria" id="ACJ76406">
        <property type="protein sequence ID" value="ACJ76406"/>
        <property type="gene ID" value="THA_1985"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1985"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052600_0_0_0"/>
    <dbReference type="OMA" id="WDHTARI"/>
    <dbReference type="OrthoDB" id="1264541at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="402" mass="45365" checksum="8805E94A95DF6144" modified="2009-02-10" version="1">MKRLLILLLVITVIMAFSVETLVVSSRLWTPPTEKEFILNEIIKPFEEMYNVKVVFQTMDDETMLKQVKVQKETGKITTDVIIAYATKMPEWVENGLVVDLTPYVSKWTDRHFSKGFDSMTVFNGKRYFLPVGADVYLTLINKKALQYKPADVDVQNLTWEQLAEWANLVAQGEGEGKFAVTGVPMKSLIYQIGAISLSYGGHWPNLDNPGAVAAWYLIYKMKDAFSPAVKTYDDTRPPMKREETWMTVAHCARVGEVYKSNPTQFIIAPAPKGPAGIGSIAGTSGLAIVNGTKHFDLALKFLEYMTRPDIMVKSHKGTGGFIPPVDEAIKYLGNDEQDEVIKNAIKVMNQGVLSYIAPIWKDWGQVKLVYDELFKKMILEEGKLDVDMLELYQLQIDAQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEJ6</accession>
    <name>B7IEJ6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA double-strand break repair protein Rad50, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579284.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2"/>
    <dbReference type="EnsemblBacteria" id="ACJ74510">
        <property type="protein sequence ID" value="ACJ74510"/>
        <property type="gene ID" value="THA_2"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2"/>
    <dbReference type="eggNOG" id="COG1122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024631_1_0_0"/>
    <dbReference type="OMA" id="CFRYLLH"/>
    <dbReference type="OrthoDB" id="1137363at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038734">
        <property type="entry name" value="YhaN_AAA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13514">
        <property type="entry name" value="AAA_27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_27" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="204"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="367"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="643" mass="75692" checksum="418C9B20658F7248" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEK0</accession>
    <name>B7IEK0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_6</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579288.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_6"/>
    <dbReference type="EnsemblBacteria" id="ACJ74514">
        <property type="protein sequence ID" value="ACJ74514"/>
        <property type="gene ID" value="THA_6"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_6"/>
    <dbReference type="eggNOG" id="COG0553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_340069_0_0_0"/>
    <dbReference type="OrthoDB" id="325071at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="401"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="667"/>
            <end position="821"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="835" mass="99010" checksum="24CCA8C448207957" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IES9</accession>
    <name>B7IES9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_85</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103439.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_85"/>
    <dbReference type="EnsemblBacteria" id="ACJ74593">
        <property type="protein sequence ID" value="ACJ74593"/>
        <property type="gene ID" value="THA_85"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_85"/>
    <dbReference type="eggNOG" id="ENOG5033E8Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192806_0_0_0"/>
    <dbReference type="OMA" id="EAEAHIM"/>
    <dbReference type="OrthoDB" id="1996008at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032587">
        <property type="entry name" value="DUF4911"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16256">
        <property type="entry name" value="DUF4911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="78" mass="9019" checksum="2AC57DA21C1EC9EB" modified="2009-02-10" version="1">MEKELDVLLKIDKEDVHLLSYILESEDNLANIRKYENQILRIITTSSLLDEVMKILNSLKEKIKFEIIEIKENSGSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEV5</accession>
    <name>B7IEV5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_111</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_111"/>
    <dbReference type="EnsemblBacteria" id="ACJ74619">
        <property type="protein sequence ID" value="ACJ74619"/>
        <property type="gene ID" value="THA_111"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_111"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1488349_0_0_0"/>
    <dbReference type="OMA" id="YSFESEI"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="181" mass="21454" checksum="934BCAB2582FF908" modified="2009-02-10" version="1">MLKNNLKAKFFELIELIYTKFYEEWEREGLFSSNSLDNFLEIIVKNLAEVDAALLSKKINNYFKIKSSYGFDNDEFMKKLLFFEDEIYTERFKKPMVVKLDEILDEYYLEVDNKRMEKVNELVKHGNLLRLTSSLSIPIFKDKECVGFLSLYSFESEIAFENNEYISFANVLSKILSSFVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEW6</accession>
    <name>B7IEW6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pstA</name>
        <name evidence="8" type="ordered locus">THA_122</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103528.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_122"/>
    <dbReference type="EnsemblBacteria" id="ACJ74630">
        <property type="protein sequence ID" value="ACJ74630"/>
        <property type="gene ID" value="THA_122"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_122"/>
    <dbReference type="eggNOG" id="COG0581">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_2_2_0"/>
    <dbReference type="OMA" id="GIARWKA"/>
    <dbReference type="OrthoDB" id="1763210at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="57"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="273" mass="29603" checksum="E0E94069A4BA5F3F" modified="2009-02-10" version="1">MDKFAKIILKILSYIVVVFIVFIIIEVLANGIKYFSLDFFTKYPENGMRQGGIFPAIVGSIYIIITSVIFSVPLGIFTGIFLAEYKDKKISKIVELAVTGLSGMPSIVYGLFGYAMFSIALGFGTSILSASLTLSIMTLPIISNSTKEAMASLPKELKESAYALGAKRNETIFKVLLKASKSRITTGVLLGLGRTLGETAPVLVTGSVFYATSMPKNIFSPVMTLPTHIYYLISAYGKESMWMTSGTAAFLLILILVIYLIAYKIGGISNGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEY9</accession>
    <name>B7IEY9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extracellular solute-binding protein, family 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_145</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103587.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_145"/>
    <dbReference type="EnsemblBacteria" id="ACJ74653">
        <property type="protein sequence ID" value="ACJ74653"/>
        <property type="gene ID" value="THA_145"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_145"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_7_2_0"/>
    <dbReference type="OMA" id="IIWGIDE"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857338" evidence="1">
        <location>
            <begin position="19"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="2">
        <location>
            <begin position="66"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="614" mass="69815" checksum="EA4AA1223BF93396" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF09</accession>
    <name>B7IF09_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable queuosine precursor transporter</fullName>
            <shortName evidence="1">Q precursor transporter</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the import of queuosine (Q) precursors, required for Q precursor salvage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family. Q precursor transporter subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103639.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_166"/>
    <dbReference type="EnsemblBacteria" id="ACJ74673">
        <property type="protein sequence ID" value="ACJ74673"/>
        <property type="gene ID" value="THA_166"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_166"/>
    <dbReference type="eggNOG" id="COG1738">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075503_1_2_0"/>
    <dbReference type="OMA" id="IFMTNVA"/>
    <dbReference type="OrthoDB" id="1462872at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990397">
        <property type="term" value="P:queuosine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02088">
        <property type="entry name" value="Q_prec_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003744">
        <property type="entry name" value="YhhQ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34300">
        <property type="entry name" value="PTHR34300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02592">
        <property type="entry name" value="Vut_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00697">
        <property type="entry name" value="TIGR00697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="210" mass="23816" checksum="A72C1E5E6B690A61" modified="2009-02-10" version="1">MEKSQERLILLTGLFTAVIIISNIIAGKLVNIGPFVITLSVLIYPLSFGISNIIFEVYGANMAKKVIKVGFLASLILVIYSYLVIFYPPSNIFDKNNAYITVFNSTPRIILASFLAYLSAQYTNIWAFNLPKKLFNYENITFRNAFSMIITQFVDSIVFVVVSFFGSYDLNVLFNMILSQYIVKLALSIFNSPIINMSVKKIKNEFAFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF27</accession>
    <name>B7IF27_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103675.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_186"/>
    <dbReference type="EnsemblBacteria" id="ACJ74691">
        <property type="protein sequence ID" value="ACJ74691"/>
        <property type="gene ID" value="THA_186"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_186"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_493418_0_0_0"/>
    <dbReference type="OMA" id="PFIVTGH"/>
    <dbReference type="OrthoDB" id="198717at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_beta_propeller_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="553" mass="63391" checksum="A16EB3825564413F" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF39</accession>
    <name>B7IF39_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">secF</name>
        <name evidence="10" type="ordered locus">THA_198</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a complex with SecD. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579406.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_198"/>
    <dbReference type="EnsemblBacteria" id="ACJ74703">
        <property type="protein sequence ID" value="ACJ74703"/>
        <property type="gene ID" value="THA_198"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_198"/>
    <dbReference type="eggNOG" id="COG0341">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050012_0_1_0"/>
    <dbReference type="OMA" id="AFEWRMA"/>
    <dbReference type="OrthoDB" id="410730at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01464_B">
        <property type="entry name" value="SecF_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022645">
        <property type="entry name" value="SecD/SecF_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022646">
        <property type="entry name" value="SecD/SecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005665">
        <property type="entry name" value="SecF_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07549">
        <property type="entry name" value="Sec_GG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01755">
        <property type="entry name" value="SECFTRNLCASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00916">
        <property type="entry name" value="2A0604s01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00966">
        <property type="entry name" value="3a0501s07"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="302" mass="33611" checksum="D00E38E5AFC4BC02" modified="2009-02-10" version="1">MIDFVGKRKIFIVISLILVVASLISIFTKGFNYGVEFLGGSEIILKVNAEVSESDVRQLIKDLAPEFENARITKIKSVDDPKGISKFSIVVSPKDENGELKVYTGEEKSVLSQKIVEAFKNNNYTAEVIGFNETSGYAAQEIKNLTWKAIVFTLLALLIYITLRFQFVFGVGAVVALIHDVVITLGFYSFFNYELNVAAVAAILTLIGYSLNDTIVVYDRIRENLRRSRGSTIETLVNNSINQVIKRTINTSLTTFIVIFILLLFSGNSIKPFAFGMTIGTIVGTYSSLYIASPIVIRWIKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF44</accession>
    <name>THYX_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flavin-dependent thymidylate synthase</fullName>
            <shortName evidence="1">FDTS</shortName>
            <ecNumber evidence="1">2.1.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">FAD-dependent thymidylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thymidylate synthase ThyX</fullName>
            <shortName evidence="1">TS</shortName>
            <shortName evidence="1">TSase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thyX</name>
        <name type="ordered locus">THA_203</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH(2) as the reductant.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP + H(+) + NADPH = (6S)-5,6,7,8-tetrahydrofolate + dTMP + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:29043"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.148"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate synthase ThyX family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.148" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579410.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IF44"/>
    <dbReference type="SMR" id="B7IF44"/>
    <dbReference type="STRING" id="484019.THA_203"/>
    <dbReference type="EnsemblBacteria" id="ACJ74708">
        <property type="protein sequence ID" value="ACJ74708"/>
        <property type="gene ID" value="THA_203"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_203"/>
    <dbReference type="eggNOG" id="COG1351">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067790_0_0_0"/>
    <dbReference type="OMA" id="EWARLMP"/>
    <dbReference type="OrthoDB" id="896350at2"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050797">
        <property type="term" value="F:thymidylate synthase (FAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01408">
        <property type="entry name" value="ThyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003669">
        <property type="entry name" value="Thymidylate_synthase_ThyX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036098">
        <property type="entry name" value="Thymidylate_synthase_ThyX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34934">
        <property type="entry name" value="PTHR34934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02511">
        <property type="entry name" value="Thy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69796">
        <property type="entry name" value="SSF69796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02170">
        <property type="entry name" value="thyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51331">
        <property type="entry name" value="THYX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000184601" description="Flavin-dependent thymidylate synthase">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="ThyX" evidence="2">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="ThyX motif" evidence="1">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="active site" description="Involved in ionization of N3 of dUMP, leading to its activation" evidence="1">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="75"/>
            <end position="78"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="86"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="172"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00661"/>
        </source>
    </evidence>
    <sequence length="228" mass="26827" checksum="B8669467D3EE97D4" modified="2009-02-10" version="1">MEYKILDKGFLRLVDMMGDDYAAVKAARVSYGKGIKTPEKDKNLIFYLMEHGHETPFEHIVFTFHVKAPIFVARQWFRHRIGSFNEASLRYTELKDEFYIPDHVRKNVVEDKQKAIKVDDEQLKQKALDLIESSIENSYKVYKELLEMGVAREMARIVLPMSSYTQFYWTVNARSLMNFLNLRADSHAQWEIQQYALNIANIFKEKCPWTFEAFLKFAYKGDLLKGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF49</accession>
    <name>B7IF49_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Chromate transport protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_208</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chromate ion transporter (CHR) (TC 2.A.51) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_208"/>
    <dbReference type="EnsemblBacteria" id="ACJ74713">
        <property type="protein sequence ID" value="ACJ74713"/>
        <property type="gene ID" value="THA_208"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_208"/>
    <dbReference type="eggNOG" id="COG2059">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018106_1_1_0"/>
    <dbReference type="OMA" id="FCGIIFP"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015109">
        <property type="term" value="F:chromate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003370">
        <property type="entry name" value="Chromate_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02417">
        <property type="entry name" value="Chromate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="153" mass="17180" checksum="211A07F32EC08B8C" modified="2009-02-10" version="1">MVPVLKRKFSKYIDEEKFAEILSFAQSMPGPIAINLAILIGEQVIGFSGILLSVLGVLIPPVSIIILAGSIIGKYSAQLHGFFEGVYASIIGLVLGVLYSIMKIQRWNLLKVVILLASVLFAVFFRKFTIYIFIFLVGSFYYVKRINKDNKKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF66</accession>
    <name>B7IF66_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_225</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103765.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_225"/>
    <dbReference type="EnsemblBacteria" id="ACJ74730">
        <property type="protein sequence ID" value="ACJ74730"/>
        <property type="gene ID" value="THA_225"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_225"/>
    <dbReference type="eggNOG" id="COG1141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_6_4_0"/>
    <dbReference type="OMA" id="DVFEMND"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001080">
        <property type="entry name" value="3Fe4S_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00352">
        <property type="entry name" value="3FE4SFRDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="60" mass="6367" checksum="10EF1E76963E56EC" modified="2009-02-10" version="1">MKVRVDEATCIGCGVCENLCPDVFKIGDDMKAKVLQAETDLDCAKDAADSCPTSAITVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFA5</accession>
    <name>B7IFA5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">FeS assembly ATPase SufC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">sufC</name>
        <name evidence="6" type="ordered locus">THA_268</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Ycf16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_268"/>
    <dbReference type="EnsemblBacteria" id="ACJ74769">
        <property type="protein sequence ID" value="ACJ74769"/>
        <property type="gene ID" value="THA_268"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_268"/>
    <dbReference type="eggNOG" id="COG0396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_48_1_0"/>
    <dbReference type="OMA" id="MAMLEPK"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="PTHR43204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01978">
        <property type="entry name" value="sufC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="5"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="247" mass="27580" checksum="22CD4E8327F33E45" modified="2009-02-10" version="1">MEKLLEVRNLHASVKEENLKILKGVNLDIGYGELHAIMGPNGSGKSTLANVIMGNPRYKVDSGEIIFEGENIVELTTDERAKKGIFMTFQNPFEIDGVKFSNFLLSAYRKIHGDSESFAQLNKQIDEILSELVVSDNFLNRFLNVGFSGGEKKKSEILQARFLKPKLLILDEIDSGLDVDALRIVANQINKIRESGTSILIITHYKRILNYLDVDKVHVYTDGKIVKSGDFSLADEIEKNGYSVIAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-05" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFC4</accession>
    <name>MIAB_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase</fullName>
            <ecNumber evidence="1">2.8.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-i(6)A37 methylthiotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">miaB</name>
        <name type="ordered locus">THA_288</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-SH + AH2 + N(6)-dimethylallyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-dimethylallyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:37067"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="Rhea" id="RHEA-COMP:10376"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="ChEBI" id="CHEBI:74417"/>
            <dbReference type="EC" id="2.8.4.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylthiotransferase family. MiaB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.3" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579472.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFC4"/>
    <dbReference type="SMR" id="B7IFC4"/>
    <dbReference type="STRING" id="484019.THA_288"/>
    <dbReference type="PRIDE" id="B7IFC4"/>
    <dbReference type="EnsemblBacteria" id="ACJ74788">
        <property type="protein sequence ID" value="ACJ74788"/>
        <property type="gene ID" value="THA_288"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_288"/>
    <dbReference type="eggNOG" id="COG0621">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_2_0_0"/>
    <dbReference type="OMA" id="CEHFHIP"/>
    <dbReference type="OrthoDB" id="397139at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035596">
        <property type="term" value="F:methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01864">
        <property type="entry name" value="tRNA_metthiotr_MiaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006463">
        <property type="entry name" value="MiaB_methiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00273">
        <property type="entry name" value="(dimethylallyl)adenosine_tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRAM"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000374611" description="tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="MTTase N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="132"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="366"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
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        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="429" mass="49529" checksum="C5FA01A0A0B13D7A" modified="2009-02-10" version="1">MKFFIKTYGCQMNENDSEVARYYLEQEGYESAENENEADIVILNTCVVRKKAEDKFLSTIGELRKKNKKIGVMGCGAEKLKEDLFKRGVNFVIGTRAISRIPEAVELSIKGKKAAIFDDKLDEIDYRNILKRNSKHHAWITIIYGCNRFCTYCIVPYTRGREKSRKMDDILREVKNLSLNGVREITYLGQNVDAYGKDLNDGTSLAKLLNETKKIENIERIWFLTSYPTDFSLDIAREIASSEKIAKSIHLPVQHGSNKILKKMNRRYTIEEYYELIKSIREIVPDASISSDIIVGFPDETEDDFQQTVKLVEEIKFERLNLAIYSPREGTIAWKYFEDNVPRAIKTRRMAYLLNLQKEINKMLNESYLDKTVEVIVEERAKSGLFYGRDIRNKIIAFEGDESLIGKKILVKIKKTTAGPLYGDIIKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFC7</accession>
    <name>B7IFC7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation efflux family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_291</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_291"/>
    <dbReference type="EnsemblBacteria" id="ACJ74791">
        <property type="protein sequence ID" value="ACJ74791"/>
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    </dbReference>
    <dbReference type="KEGG" id="taf:THA_291"/>
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    </dbReference>
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    <dbReference type="OMA" id="IEMHLIV"/>
    <dbReference type="OrthoDB" id="381612at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
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        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF160240"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CDF"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
    </feature>
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        </location>
    </feature>
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            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="ZT_dimer" evidence="7">
        <location>
            <begin position="213"/>
            <end position="288"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ACJ74791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="304" mass="34303" checksum="26143DB77F5CD5F2" modified="2009-02-10" version="1">MEKNIKIVTSIAVVVNTFLAALKIIIGFLFNSMAVLADGIDSATDILTASIVYFATRLSSKPPDKLHPYGHRKIENIGAKIISFIVFYAGISLLIESIKRLVTHNYTLIQGIIPITVTLISVIFKTFLFIIEYRIGKKYNRPSLIAEALNMRNDILLSSIVFFGILLNKIGLSFMDPLVGMIMSVIIVKVAFEIFNENAVALLDGIKKDEEWIYDEIINLCNECEGVKNPHKIRIRKIGECFDIDMDIEVDPKINVQKSHELTLCIKDKIKKLLNDKVYDVVIHVEPYQNVENEPYGIGGKNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFE2</accession>
    <name>B7IFE2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heat-inducible transcription repressor HrcA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">hrcA</name>
        <name evidence="8" type="ordered locus">THA_306</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
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        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HrcA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103915.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_306"/>
    <dbReference type="EnsemblBacteria" id="ACJ74806">
        <property type="protein sequence ID" value="ACJ74806"/>
        <property type="gene ID" value="THA_306"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_306"/>
    <dbReference type="eggNOG" id="COG1420">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050019_1_0_0"/>
    <dbReference type="OMA" id="LNWEEIE"/>
    <dbReference type="OrthoDB" id="1510750at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00081">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002571">
        <property type="entry name" value="HrcA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021153">
        <property type="entry name" value="HrcA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005104">
        <property type="entry name" value="WHTH_HrcA_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023120">
        <property type="entry name" value="WHTH_transcript_rep_HrcA_IDD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34824">
        <property type="entry name" value="PTHR34824"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01628">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03444">
        <property type="entry name" value="HrcA_DNA-bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005485">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00331">
        <property type="entry name" value="hrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0678">Repressor</keyword>
    <keyword evidence="3 5" id="KW-0346">Stress response</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HrcA_DNA-bdg" evidence="7">
        <location>
            <begin position="11"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="HrcA" evidence="6">
        <location>
            <begin position="114"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="345" mass="40066" checksum="F0335714CE543AC6" modified="2009-02-10" version="1">MADKWGEKMKELSTRQEQILYCLVREYVKSGTPVSSQKILESTNLEWSGATVRNDMRKLDYLGYVFQPHTSAGRVPTDKGLRFYVNEILKLRKETKKSGSSIDVSIDFPIGDLDKIIQGAAKLLATTVKAFVIIEKPNPMYLRIRRVVLTPVTKNFSIVNIITELGLTSVLPIQHSEIYNIQEIEEFLNKSLDGILLSEFRAKLREVVERFSWVGGRLKEFIELSEKIASEKYEEYITEGVFNLVNAKRFDEEKLKEIVRFSTNEEYYSKIFELGEGIFIGKEHNIKNFEQYSILIMPYFVFNREVGKIAVIFDKFSDYNRVFDSVEYVVNRLTEYFTVVARNVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFF0</accession>
    <name>B7IFF0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Crispr-associated ramp protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_314</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579493.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_314"/>
    <dbReference type="EnsemblBacteria" id="ACJ74814">
        <property type="protein sequence ID" value="ACJ74814"/>
        <property type="gene ID" value="THA_314"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_314"/>
    <dbReference type="eggNOG" id="COG1367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_645474_0_0_0"/>
    <dbReference type="OrthoDB" id="1800053at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007522">
        <property type="entry name" value="CRISPR-assoc_prot_TM1795"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01894">
        <property type="entry name" value="cas_TM1795_cmr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="406" mass="47928" checksum="858FBFFFC92ABAEE" modified="2009-02-10" version="1">MKNLETLKFNIEFISPLINRRGKYKDSILLPQTVRGVMRYYFRATAARVFGNNYKKIKELEDIIFGSINNKSFFDVTVTLQKERKIKLSELKALRYPLYGVIFTKKNEIKFNEKISLLDSRSEFLVNFIFFKSFFKRKIKDCNKVDEYFHKFKEFIKDLFVLISLVGGFGAKVKKGFGQFVIRNIERDIKLEDIGTILKNLEEKIKYLDKIFIKENEKNKESITNNSSSISNEAFDFPAFVDGFFYVYKLKKEFYSYENALRNIYFSKKNNFSYLNLKKKLRGSAFKDAIQNFNDKKKVDFNDALLGLPVLFFYGKANYKLNALDDKGNEYRKSSLLTIKLWRERNGKYKVYLIVLLSKIHLKNSKIVLKKIVQTNGSEGKSINIDFDYNKLKNTIEKYKELNKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFN9</accession>
    <name>B7IFN9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fe-S oxidoreductase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579558.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_410"/>
    <dbReference type="EnsemblBacteria" id="ACJ74903">
        <property type="protein sequence ID" value="ACJ74903"/>
        <property type="gene ID" value="THA_410"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_410"/>
    <dbReference type="eggNOG" id="COG1032">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011543_3_2_0"/>
    <dbReference type="OMA" id="WYTTRER"/>
    <dbReference type="OrthoDB" id="970297at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023862">
        <property type="entry name" value="CHP03960_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045784">
        <property type="entry name" value="Radical_SAM_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19864">
        <property type="entry name" value="Radical_SAM_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03960">
        <property type="entry name" value="rSAM_fuse_unch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="244"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="592" mass="68689" checksum="B60E69E71A22DE14" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFP2</accession>
    <name>CRCB_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative fluoride ion transporter CrcB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">crcB</name>
        <name type="ordered locus">THA_413</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Important for reducing fluoride concentration in the cell, thus reducing its toxicity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CrcB (TC 9.B.71) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104149.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFP2"/>
    <dbReference type="SMR" id="B7IFP2"/>
    <dbReference type="STRING" id="484019.THA_413"/>
    <dbReference type="EnsemblBacteria" id="ACJ74906">
        <property type="protein sequence ID" value="ACJ74906"/>
        <property type="gene ID" value="THA_413"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_413"/>
    <dbReference type="eggNOG" id="COG0239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114342_2_3_0"/>
    <dbReference type="OMA" id="FLMFESF"/>
    <dbReference type="OrthoDB" id="1942863at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903425">
        <property type="term" value="F:fluoride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00454">
        <property type="entry name" value="CrcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="CrcB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="PTHR28259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00494">
        <property type="entry name" value="crcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_1000125162" description="Putative fluoride ion transporter CrcB">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00454"/>
        </source>
    </evidence>
    <sequence length="125" mass="13882" checksum="A1CF1FEE02D19C4C" modified="2009-02-10" version="1">MKSLYVAFGGALGAVSRYVISKFINSSFSFSFVPWGTIFVNVVGSFLLSFLMFLSISKTDISQSFILFFGTGFLGAFTTFSTFAYEFLSIFLTQPLRAIIYFIANIFLGFFAAILGMFLGRGRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFQ2</accession>
    <name>B7IFQ2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alcohol dehydrogenase, zinc-binding</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_423</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579569.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_423"/>
    <dbReference type="EnsemblBacteria" id="ACJ74916">
        <property type="protein sequence ID" value="ACJ74916"/>
        <property type="gene ID" value="THA_423"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_423"/>
    <dbReference type="eggNOG" id="COG1064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_826176_0_0_0"/>
    <dbReference type="OMA" id="GKMQNPV"/>
    <dbReference type="OrthoDB" id="1083526at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="345" mass="37296" checksum="A326DB75DC56369A" modified="2009-02-10" version="1">MINKRGCPYGTHRVIEPKGTLPQAAKKIDNTMEIYTNEILIDVKTLNVDSASFTQIKESCNKDIDCMKKTILSIVNERGKLQNPVTGSGGMLIGVVEEIGSDLKTDLKVGDKIATLVSLSLTPLKIDEILNIKIDADQVDIKGKAILFETGIYAKLPDDIPEKLALAVLDVAGAPAQVNKLVKKGMTVAIIGAGGKSGLLCCYQAMKNVGSSGRVIAIEYSQENAEKVRKLNLAHDVIVADATKPVEVYNEFLKVTGGQLADVTINNVNVPTTEMSSILITKDEGIIYFFSMATSFTRAALGAEGVGKDVTMIIGNGYTKGHAELSLNILRESKEIRKLFEEKYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFR3</accession>
    <name>B7IFR3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_434</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579578.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_434"/>
    <dbReference type="EnsemblBacteria" id="ACJ74927">
        <property type="protein sequence ID" value="ACJ74927"/>
        <property type="gene ID" value="THA_434"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_434"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_616664_0_0_0"/>
    <dbReference type="OMA" id="ACLERIT"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="222"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="444" mass="52894" checksum="D0A9536531BEA9D7" modified="2009-02-10" version="1">MKKIILIFIFVFSSLIFSNMQEEIRNYVENSPEYLQALKNYINSSYEKGYSLSFGTNLNKVSFDYDFDSYQLSVPVTFKFSDDFSVSANFSANKDVLTFPVEIDFNVFNFDINLSGNFRFVNDEFKDSYGISISKNLFDNSDEEDLEEKISLLNAKWNLLNVKNQKVIEYLNNAYYSLYYSKMYEIYSKLYQLKKEEFKKVEEKYSKGIISQIEYLNEKKSLLMLQKNLKSYESKKSEYEKYSGFPLSIFEVNLPQKDFGKRLDLKALEIQKQLNELKKERLYQLYVPNINLGFSIDYDYKEGSSDKEFKPKVYLSFTLDLFGNKKLEKENILIDYEIASKNLQEKIDMLENQYEQFFYELENSNNDYEIALIEKKIKEINLEKVKEKFEKGLVTENEYKISEYEYLIEEYNLENVEFSIFIKKLNIYSFLGYDLLSLIEEVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFS6</accession>
    <name>B7IFS6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA repair protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_449</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the UPF0758 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104216.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_449"/>
    <dbReference type="EnsemblBacteria" id="ACJ74940">
        <property type="protein sequence ID" value="ACJ74940"/>
        <property type="gene ID" value="THA_449"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_449"/>
    <dbReference type="eggNOG" id="COG2003">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073529_0_2_0"/>
    <dbReference type="OMA" id="AMPDYEL"/>
    <dbReference type="OrthoDB" id="1833204at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08071">
        <property type="entry name" value="MPN_DUF2466"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025657">
        <property type="entry name" value="RadC_JAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001405">
        <property type="entry name" value="UPF0758"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020891">
        <property type="entry name" value="UPF0758_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30471">
        <property type="entry name" value="PTHR30471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04002">
        <property type="entry name" value="RadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00608">
        <property type="entry name" value="radc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01302">
        <property type="entry name" value="UPF0758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="7">
        <location>
            <begin position="86"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="213" mass="24074" checksum="5369131691370E34" modified="2009-02-10" version="1">MLPREKLINEGVENLSTEELLAILLRTGTKDLDVLKLSKNLFETFDKSLKKISTASIDELCKVKGLGTVKAVTILAAMELSKRYLLEDRKGKFLNSPELIYEHCLDMKHFEQEVVRVISLNSKLYEISTKDITIGLTDTSLAHPREIYREAIKNSASYVVVVHNHPSGDVRPSKEDREVTLKIKKAGEIIGIKLLDHVIIGNGFYSFKHNKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFT6</accession>
    <name>B7IFT6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA (adenine(58)-N(1))-methyltransferase TrmI</fullName>
            <ecNumber evidence="4">2.1.1.220</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_459</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the S-adenosyl-L-methionine-dependent formation of N(1)-methyladenine at position 58 (m1A58) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>adenosine(58) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methyladenosine(58) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10365"/>
            <dbReference type="Rhea" id="RHEA-COMP:10366"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
            <dbReference type="EC" id="2.1.1.220"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer composed of a dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.220" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579596.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_459"/>
    <dbReference type="EnsemblBacteria" id="ACJ74950">
        <property type="protein sequence ID" value="ACJ74950"/>
        <property type="gene ID" value="THA_459"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_459"/>
    <dbReference type="eggNOG" id="COG2519">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025402_0_1_0"/>
    <dbReference type="OMA" id="VVYPKDA"/>
    <dbReference type="OrthoDB" id="944891at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016429">
        <property type="term" value="F:tRNA (adenine-N1-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="PTHR12133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR017269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="256" mass="29368" checksum="282E106EEC5C1F4C" modified="2009-02-10" version="1">MIKEGDKVLLYGDDGSKIIITVEAGKKKGTHLGHVDMDELIGKEYGDTIVFGKNNRVFYILKPTYIDLIFNMKRRTQIIYPKDASYILFKLDIKPGDRVIDTGVGSGAMCGAFARIVGKDGKVYGYERREDFYNLAKKNLEYWGLIDNVELKLRDISGGFDEVNVDALMLDVPDPQNYIEQCWQALKGGGKMGIICPTTNQVQEVLEKLYEYPFIDVEVWENLMRRYKPNPERLRPFDRMVAHTTYLVFATKVLKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFU1</accession>
    <name>HSLV_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent protease subunit HslV</fullName>
            <ecNumber evidence="1">3.4.25.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hslV</name>
        <name type="ordered locus">THA_464</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent cleavage of peptide bonds with broad specificity.</text>
            <dbReference type="EC" id="3.4.25.2"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by HslU binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase T1B family. HslV subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.2" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104244.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFU1"/>
    <dbReference type="SMR" id="B7IFU1"/>
    <dbReference type="STRING" id="484019.THA_464"/>
    <dbReference type="MEROPS" id="T01.006"/>
    <dbReference type="EnsemblBacteria" id="ACJ74955">
        <property type="protein sequence ID" value="ACJ74955"/>
        <property type="gene ID" value="THA_464"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_464"/>
    <dbReference type="eggNOG" id="COG5405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093872_1_0_0"/>
    <dbReference type="OMA" id="IMKGNAR"/>
    <dbReference type="OrthoDB" id="1129370at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009376">
        <property type="term" value="C:HslUV protease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01913">
        <property type="entry name" value="protease_HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00248">
        <property type="entry name" value="HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022281">
        <property type="entry name" value="ATP-dep_Prtase_HsIV_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="PTHR32194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039093">
        <property type="entry name" value="HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03692">
        <property type="entry name" value="ATP_dep_HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0021">Allosteric enzyme</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0915">Sodium</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <keyword id="KW-0888">Threonine protease</keyword>
    <feature type="chain" id="PRO_1000192687" description="ATP-dependent protease subunit HslV">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="6"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00248"/>
        </source>
    </evidence>
    <sequence length="176" mass="19292" checksum="E4EA6CB260BC22A3" modified="2009-02-10" version="1">MKWRSTTVVCVRRNDSVVMISDGQVTYGNTILKGNAKKVRKMGDGNVLAGFAGSVADAMALFDRFEAKYREWGGNLLKAAVELAKDWRTDRVLRRLEAMLLVADKKYTFIVSGTGEVIQPEDDIASIGSGSPYAIAAGKALLRHTDLSAKEIALEAIKIASEICIYTNDNFTIEEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFU6</accession>
    <name>TRMFO_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO</fullName>
            <ecNumber evidence="1">2.1.1.74</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Folate-dependent tRNA (uracil-5-)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Folate-dependent tRNA(M-5-U54)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmFO</name>
        <name type="ordered locus">THA_469</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH + uridine(54) in tRNA = (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine(54) in tRNA + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:16873"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.74"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH + uridine(54) in tRNA = (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine(54) in tRNA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:62372"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.74"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MnmG family. TrmFO subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.74" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579598.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFU6"/>
    <dbReference type="SMR" id="B7IFU6"/>
    <dbReference type="STRING" id="484019.THA_469"/>
    <dbReference type="EnsemblBacteria" id="ACJ74960">
        <property type="protein sequence ID" value="ACJ74960"/>
        <property type="gene ID" value="THA_469"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_469"/>
    <dbReference type="eggNOG" id="COG1206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033057_1_0_0"/>
    <dbReference type="OMA" id="MHRNTFL"/>
    <dbReference type="OrthoDB" id="516534at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030698">
        <property type="term" value="F:5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047151">
        <property type="term" value="F:methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01037">
        <property type="entry name" value="TrmFO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004417">
        <property type="entry name" value="TrmFO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="PTHR11806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF2">
        <property type="entry name" value="PTHR11806:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00137">
        <property type="entry name" value="gid_trmFO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000135892" description="Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01037"/>
        </source>
    </evidence>
    <sequence length="429" mass="49015" checksum="4A643E3D670B077F" modified="2009-02-10" version="1">MRVNIVGAGLAGVEVAYKLLREGFKVRIFEQKPVKFSPVHKMETFGELVCSNSLKSESLKNAEGILKEEMKLLDSLVLNCAYKTRVPAGKALAVDREKFSQCITKVLESFENIEIVRKEVEKIDLDTNEIWVVATGPTTDGKFAQWLSKLTGGFLNFFDAVAPIISRDSIDFNKCFVGDRYGVGTGDYINCPMTKEEYERFYRELVNAEMIEMKDFDRKLLFERCQPIEEIAKSGEKSLLFGPLRPVGLVNPHTGEIPYAVIQLRKEDEEGNMYNIVGFQTRLKWSEQKRIIRLIPGLENAEILRYGVMHRNTYIDTPRVLDEFLRHKKYKNIFFAGQITGVEGYLESAACGIYVGLNISRILSGKEPVKLPPKTMMGALINYITKADELKPMYANFGLINVKMKKNEKREKLHEICINEMKNFLNMLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFV7</accession>
    <name>CSRA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translational regulator CsrA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">csrA</name>
        <name type="ordered locus">THA_480</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">A translational regulator that binds mRNA to regulate translation initiation and/or mRNA stability. Usually binds in the 5'-UTR at or near the Shine-Dalgarno sequence preventing ribosome-binding, thus repressing translation. Its main target seems to be the major flagellin gene, while its function is anatagonized by FliW.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer; the beta-strands of each monomer intercalate to form a hydrophobic core, while the alpha-helices form wings that extend away from the core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CsrA/RsmA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104262.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFV7"/>
    <dbReference type="SMR" id="B7IFV7"/>
    <dbReference type="STRING" id="484019.THA_480"/>
    <dbReference type="EnsemblBacteria" id="ACJ74971">
        <property type="protein sequence ID" value="ACJ74971"/>
        <property type="gene ID" value="THA_480"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_480"/>
    <dbReference type="eggNOG" id="COG1551">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164837_0_1_0"/>
    <dbReference type="OMA" id="IHRKEVY"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048027">
        <property type="term" value="F:mRNA 5'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902208">
        <property type="term" value="P:regulation of bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006109">
        <property type="term" value="P:regulation of carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00167">
        <property type="entry name" value="CsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003751">
        <property type="entry name" value="CsrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036107">
        <property type="entry name" value="CsrA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34984">
        <property type="entry name" value="PTHR34984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02599">
        <property type="entry name" value="CsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117130">
        <property type="entry name" value="SSF117130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00202">
        <property type="entry name" value="csrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <feature type="chain" id="PRO_1000118242" description="Translational regulator CsrA">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00167"/>
        </source>
    </evidence>
    <sequence length="73" mass="8337" checksum="01A58461F87EF30D" modified="2009-02-10" version="1">MLVLSRKIGQSIIIGNDIEIKILKIDGGEIKIGIEAPKDVKVLRKELYEELLKENKEAVKFDIKNLPGFFKKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFW5</accession>
    <name>MTNA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="1">M1Pi</shortName>
            <shortName evidence="1">MTR-1-P isomerase</shortName>
            <ecNumber evidence="1">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mtnA</name>
        <name type="ordered locus">THA_488</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 1/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579614.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFW5"/>
    <dbReference type="SMR" id="B7IFW5"/>
    <dbReference type="STRING" id="484019.THA_488"/>
    <dbReference type="PRIDE" id="B7IFW5"/>
    <dbReference type="EnsemblBacteria" id="ACJ74979">
        <property type="protein sequence ID" value="ACJ74979"/>
        <property type="gene ID" value="THA_488"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_488"/>
    <dbReference type="eggNOG" id="COG0182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016218_1_2_0"/>
    <dbReference type="OMA" id="RLWVDET"/>
    <dbReference type="OrthoDB" id="1290468at2"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00874"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019284">
        <property type="term" value="P:L-methionine salvage from S-adenosylmethionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000187369" description="Methylthioribose-1-phosphate isomerase">
        <location>
            <begin position="1"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="48"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="247"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="346" mass="38047" checksum="6D3FAE1BB046FD85" modified="2009-02-10" version="1">MKLKTMTMEWTGDELILIDQRKIPLVEEYVSCKSYKEVAVAIKDMVVRGAPAIGASAAFGYVLGAREMFVEDFNAFVKKMEEVKEVLANTRPTAVNLFWALNRMEDSLKKYGKIEGILEYLEEEAMNIAKEDIEVNKAIGRYGAELLKDGDTVLTHCNAGALATVDYGTALGVIRAAVEQGKKIKVFADETRPYLQGARLTAWELMKDGIDVTLISDNMSGWSMKLGKINAVIVGADRVAANGDVANKIGTYMVAVLAKRHGIPFYVAAPTSTIDLNTKTGKDIPIEERKHTEVTHCGGKQIAPDGVKVFNPAFDVTDAELVTAIITEKGVVYPPYEENLKKLFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFW8</accession>
    <name>B7IFW8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="9">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 15" type="primary">leuS</name>
        <name evidence="15" type="ordered locus">THA_491</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104295.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_491"/>
    <dbReference type="EnsemblBacteria" id="ACJ74982">
        <property type="protein sequence ID" value="ACJ74982"/>
        <property type="gene ID" value="THA_491"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_491"/>
    <dbReference type="eggNOG" id="COG0495">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004427_0_0_0"/>
    <dbReference type="OMA" id="TFMVLAP"/>
    <dbReference type="OrthoDB" id="32262at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_B">
        <property type="entry name" value="Leu_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="PTHR43740"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1g" evidence="13">
        <location>
            <begin position="40"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-synt_1_2" evidence="14">
        <location>
            <begin position="220"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-synt_1" evidence="11">
        <location>
            <begin position="419"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="12">
        <location>
            <begin position="661"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="9">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="9">
        <location>
            <begin position="580"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="583"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ACJ74982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="825" mass="95595" checksum="19DFD8B4D24E1079" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFX6</accession>
    <name>B7IFX6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zinc metalloprotease YluC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">THA_501</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_501"/>
    <dbReference type="EnsemblBacteria" id="ACJ74990">
        <property type="protein sequence ID" value="ACJ74990"/>
        <property type="gene ID" value="THA_501"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_501"/>
    <dbReference type="eggNOG" id="COG0750">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025778_1_0_0"/>
    <dbReference type="OMA" id="FAKIFKV"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004387">
        <property type="entry name" value="Pept_M50_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42837">
        <property type="entry name" value="PTHR42837"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="11" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="96"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ74990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="469" mass="52381" checksum="A40D49D1B6529FFD" modified="2009-02-10" version="1">MFAKIFKVSVLEFSIGFGPSIFKKKFGETVWKINIIPFGGYVRLKGEDFDESEEDGLYAKPAWQRLLIAFAGPLFSVLAAYILFIPIVLNWGVPAVTIGKVIENSPAQEYGLQPGDVIYKLNGKRIFDSYEVTNTVSKGKVVKMEILRNGEIIKKEIPPRISPPEYIFLLDDVKGDINGEIVKINGYKVGIDLKLESGTFVELEDEFGNTISGVLVQYSYVPERPTIGFYYAGFKPVVEKDMHPFKKGDIILKVNNREIDDYISLVNVITAMKLSENQVYMDIWGQQIREKIVPLGNDLNVLVSRNGKEISLNLNKKEFLNIISSPGFFKQESYVIKPKNFFDAISLAVARCNSAAISIWKAFGKLFTTGQGVEQVAGPVGIAVIVGEAAKAGWETILTVVALFTLNLGIFNLLPLPALDGGRIVFSLIEIISRKKVNRKVEAIVHTIGFFILMALAFYFMFADFSKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG45</accession>
    <name>B7IG45_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP synthase, Delta/Epsilon chain, beta-sandwich domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579655.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_572"/>
    <dbReference type="EnsemblBacteria" id="ACJ75059">
        <property type="protein sequence ID" value="ACJ75059"/>
        <property type="gene ID" value="THA_572"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_572"/>
    <dbReference type="eggNOG" id="COG0355">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084338_1_0_0"/>
    <dbReference type="OMA" id="DGFLHCD"/>
    <dbReference type="OrthoDB" id="1696893at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020546">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036771">
        <property type="entry name" value="ATPsynth_dsu/esu_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02823">
        <property type="entry name" value="ATP-synt_DE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="SSF51344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-synt_DE_N" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="91" mass="10324" checksum="89FE420CEE7705FF" modified="2009-02-10" version="1">MKLKIYSPNGLMFEKDVTVVTVRTVEGEMGLLERRAPIIAKLRVDKVVAKDNTQTYEYVIDDGFLHCDGENVIIVTEDIKKEIDPHKYLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG58</accession>
    <name>B7IG58_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579660.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_585"/>
    <dbReference type="EnsemblBacteria" id="ACJ75072">
        <property type="protein sequence ID" value="ACJ75072"/>
        <property type="gene ID" value="THA_585"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_585"/>
    <dbReference type="eggNOG" id="ENOG502Z7RZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1703385_0_0_0"/>
    <dbReference type="OMA" id="IYMACIE"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="154" mass="17805" checksum="992AFB47C1D678BA" modified="2009-02-10" version="1">MSLKNKNLLMMILAIALSIITYFLFREMYYSNSDFPYSQEILAAFIGTVITILITSLLLNKQTEVEMLKEENLKILELKASIYNELFSYLEEVFLKQEVTKSDIIKLKLINQKLSTIASIDVLKIFEKFIKIKANKTPSSIGWRGICIFVHNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG74</accession>
    <name>B7IG74_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_601</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579674.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_601"/>
    <dbReference type="EnsemblBacteria" id="ACJ75088">
        <property type="protein sequence ID" value="ACJ75088"/>
        <property type="gene ID" value="THA_601"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_601"/>
    <dbReference type="eggNOG" id="COG1672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041137_3_0_0"/>
    <dbReference type="OMA" id="YNEDKFH"/>
    <dbReference type="OrthoDB" id="801313at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004256">
        <property type="entry name" value="DUF234_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03008">
        <property type="entry name" value="DUF234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_2" evidence="1">
        <location>
            <begin position="3"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="DUF234" evidence="2">
        <location>
            <begin position="314"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="467" mass="55365" checksum="905A5EAB7E1F1A2F" modified="2009-02-10" version="1">MKFVDRIDEIKFLEKEYKRKTSSFIVLYGRRRVGKTRLIKEFIKNKDSVYFLATEESEAENIKTFQNILYSKYKIPLLDNNKLLSWNDLFYIISTLKLEKKLIIVIDEFQYLLKSNKGFSSILQKSWDEYLKDKNIMLIISGSALSMIKREVLSYSSPLYGRRTGQMNLKPIKFKYFKEFFENKNIDLIKLYSLTGGIPKYVEILELKENIYDTIKENFLNVNSYLFEEPYFLLEKELKDIGSYFSIIKAIANGNNKLSKISTSLGIKQTSLSYYLNNLIELDILEREVPILEKNPQTSKKGIYKIKDNFLNFWFKFIYPYKSYIEIGNIDFVMNIIKKSFIERHVSFIYEDISKEKLIDLNLNDKLPVKLSKIGRWWDKNLEIDIVGVDRENKPILFGECKYTKKPVDLDVYYALVEKSKKLLKDNNQNNLYFAFFSYNGYTKNFIDKVKKEKNILIFEGDLSENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG75</accession>
    <name>B7IG75_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA_14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_603</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579675.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75089">
        <property type="protein sequence ID" value="ACJ75089"/>
        <property type="gene ID" value="THA_603"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_603"/>
    <dbReference type="eggNOG" id="COG1373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058017_0_0_0"/>
    <dbReference type="OMA" id="KIFLHNT"/>
    <dbReference type="OrthoDB" id="801313at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_14" evidence="1">
        <location>
            <begin position="39"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="389" mass="45898" checksum="E227ADDF728ABC4E" modified="2009-02-10" version="1">MKKVEILEKFEEKKERMINFLPNKKRLYFENIEKFENERGLLIYGPRGVGKTTYLLMSAKKNNLFYVSGDDPLFSQISLYDLGESVFQEGVNGLIVDEVHYLNNWSIQIKSLYDSFPNKKIWISDSSSIILRKGISDLSRRFVKVKLPLLSFREYLYFETGKKFNKISSPFDFDREYVISLTKDVEILKYFKNYSTSGTRPFYIEGNFSEKMKNVLEKTIYSDIPYFLETIRDNHLRLMWAIISYLIYSKIPTVNVESMCREWNVGKQKLYEILYVLQESGLINIVNKKENYKVNTKGDKIFLSDPSFYHIFDGELGNFREAFVVFALKEIGKIYSIKNERDGDFIFDNYKIEVGGKNKKLKNSDFVIRDDLDIPIKNKIPLWVLGFLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG83</accession>
    <name>B7IG83_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Carbon starvation-induced protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">THA_611</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptide transporter carbon starvation (CstA) (TC 2.A.114) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579680.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_611"/>
    <dbReference type="EnsemblBacteria" id="ACJ75097">
        <property type="protein sequence ID" value="ACJ75097"/>
        <property type="gene ID" value="THA_611"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_611"/>
    <dbReference type="eggNOG" id="COG1966">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010531_4_1_0"/>
    <dbReference type="OMA" id="GTISGFH"/>
    <dbReference type="OrthoDB" id="457782at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025299">
        <property type="entry name" value="CstA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003706">
        <property type="entry name" value="CstA_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02554">
        <property type="entry name" value="CstA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13722">
        <property type="entry name" value="CstA_5TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="388"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="436"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="460"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="492"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="524"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="CstA" evidence="9">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="CstA_5TM" evidence="10">
        <location>
            <begin position="388"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="561" mass="60925" checksum="1C42DF8C3E325A72" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGC2</accession>
    <name>B7IGC2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Anaerobic glycerol 3-phosphate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_659</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579707.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_659"/>
    <dbReference type="EnsemblBacteria" id="ACJ75136">
        <property type="protein sequence ID" value="ACJ75136"/>
        <property type="gene ID" value="THA_659"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_659"/>
    <dbReference type="eggNOG" id="COG0579">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2906">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024775_3_1_0"/>
    <dbReference type="OMA" id="GVHFTRM"/>
    <dbReference type="OrthoDB" id="1371190at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007419">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041854">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04324">
        <property type="entry name" value="Fer2_BFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="1">
        <location>
            <begin position="3"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Fer2_BFD" evidence="2">
        <location>
            <begin position="401"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="478" mass="53093" checksum="8002B0812116E787" modified="2009-02-10" version="1">MKVFVIGAGISGSLIARELSKYDCEVHILEKAPDIGWGVTKANSAIVHGGYDDPPQSVRSKFAAKGNRLYDELSKELDFDFERVGSLVVAFKEEDINYLKELKERGKKNGVDDLEILEKSELKKIEPNISDEAIAALYCKSAGITEPWEVAIAAIENAVENGAILHLEEEVLDIQTSNQKVSKVITNKGEYEADVVINAAGLFADEIAKMAGVGDFEIFPRKGEYILLDKKLKGLVNTVVFPTPTKKSKGILVVPTVDGGILLGPNAKDLPKNAKYDLDTTPEGLQEVYEKSKRLIPKVDLSYTVKTFAGLRPETKEKDFIISATKIWGFVNVAGIRSPGLTAAPSFAKYVVEDILQDQLKINLSKKKDFNPYRKRITNIRNVSLKEWEELVKKDPKYGRTVCFCNNVSEAEIVEAIRRGARTLDGVKFRTRASFGRCQGGFCSLKIVEIISRELNIPMEEVKLNSKNSWIMDGKVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGC3</accession>
    <name>B7IGC3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gidA1</name>
        <name evidence="2" type="ordered locus">THA_660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579708.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_660"/>
    <dbReference type="EnsemblBacteria" id="ACJ75137">
        <property type="protein sequence ID" value="ACJ75137"/>
        <property type="gene ID" value="THA_660"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_660"/>
    <dbReference type="eggNOG" id="COG0446">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030705_0_0_0"/>
    <dbReference type="OMA" id="HPGFGLH"/>
    <dbReference type="OrthoDB" id="912110at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="7"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="408" mass="45334" checksum="D0E1A4AE3D920A03" modified="2009-02-10" version="1">MRREKVDVLIVGGGAAGLAAAISATESGAKTVLLERDRSTGGVLNQCIHNGFGLHVFKEELTGPEFMERLWEELKEDVIRPNFTVLEIKPDTREVITLSEDGIIVFEPKALVMSTGARERPLNSLRIPGSRVAGIYTAGVAQKMVNIYNRLPGKKVLIVGSGDIGLIMARRLKIEGMDVVGVVEIMPEPGGLIRNVVQCLEDFNIPLWLSHTVIEIHGKERLSEVVIAKVDENKKPIAGTEKVIKVDTLVTSVGLIPQNELIENFVEMDPINRGPVVDDYMRTSVEWIFAAGNNVAIHDLVDFVYDEGKIAGMYAARYALGEKLPEVKYEFVRGKNVGVMLPQRFTGTQPFKIYIRPTKSLVKSALKFSDKTIRKFNWRIRPSEMIDLVVKDFEGFDSKITVEVSSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGG3</accession>
    <name>B7IGG3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amine oxidase, flavin-containing superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_706</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579742.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_706"/>
    <dbReference type="EnsemblBacteria" id="ACJ75177">
        <property type="protein sequence ID" value="ACJ75177"/>
        <property type="gene ID" value="THA_706"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_706"/>
    <dbReference type="eggNOG" id="COG1233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_510694_0_0_0"/>
    <dbReference type="OMA" id="NTCMISI"/>
    <dbReference type="OrthoDB" id="418913at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amino_oxidase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="518" mass="59293" checksum="05866067A648AFC9" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGH2</accession>
    <name>B7IGH2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="5 8">5.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">galE</name>
        <name evidence="10" type="ordered locus">THA_716</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="5 8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104635.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_716"/>
    <dbReference type="EnsemblBacteria" id="ACJ75186">
        <property type="protein sequence ID" value="ACJ75186"/>
        <property type="gene ID" value="THA_716"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_716"/>
    <dbReference type="eggNOG" id="COG1087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_10_0"/>
    <dbReference type="OMA" id="GEHLICN"/>
    <dbReference type="OrthoDB" id="1597849at2"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="9">
        <location>
            <begin position="4"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="324" mass="36896" checksum="9495C0A7D233652E" modified="2009-02-10" version="1">MTVLVAGGAGYIGSHVCKMLRERGYDVVVIDNLSHGYKSFTRYGEFVLGDISDENLLDLVFKTYKIDAVMHFCAYIEVGESVVDPNKYYQNNVSNTLTLLNSMLKHDVKYFIFSSTAAVYGMPQRIPIKEDDPKMPINPYGKSKYMVEQILDDFDRAYGLKSIRFRYFNAAGADESLEIGEAHEPETHLIPLILDAALSVRDSIKIFGTDYETKDGTCIRDFVHVNDLADAHIKGLEYLISEKKTDYFNLGSGSGFSVREVIEKVKEVTNVDFKVEEVDRRPGDPAYLIADNTKARKILGWEPKYDLEKIIQTAWNWHKVLRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGK0</accession>
    <name>B7IGK0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_748</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579773.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_748"/>
    <dbReference type="EnsemblBacteria" id="ACJ75214">
        <property type="protein sequence ID" value="ACJ75214"/>
        <property type="gene ID" value="THA_748"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_748"/>
    <dbReference type="HOGENOM" id="CLU_1539298_0_0_0"/>
    <dbReference type="OrthoDB" id="842576at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="174" mass="21040" checksum="238CA7DE8E6D30FD" modified="2009-02-10" version="1">MKNETINIHPLDVKNYIEKHLNNIFEENIKIDVDEKRNTYIINKIKEVEIYDFWNKEDEELLKDFENYSFKGDCIGTIFFFLSGFWEYTHTYLKDNFGRFPAIESFQYKKNVLEEPVADILIDKLSKELNLTYKNFYTRKNVFITHDVDILQGFKGFRFMRSLVGDVLKRKKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGN4</accession>
    <name>B7IGN4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Fe-hydrogenase, subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_786</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579794.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_786"/>
    <dbReference type="EnsemblBacteria" id="ACJ75248">
        <property type="protein sequence ID" value="ACJ75248"/>
        <property type="gene ID" value="THA_786"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_786"/>
    <dbReference type="eggNOG" id="COG1894">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014881_3_2_0"/>
    <dbReference type="OMA" id="AFMNRTL"/>
    <dbReference type="OrthoDB" id="688908at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="SSF140490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="SSF142019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="567"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="597"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="624" mass="68672" checksum="7F3D309A8FCCBAB6" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGP9</accession>
    <name>B7IGP9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar motor switch protein FliG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">fliG</name>
        <name evidence="13" type="ordered locus">THA_801</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FliG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579805.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_801"/>
    <dbReference type="EnsemblBacteria" id="ACJ75263">
        <property type="protein sequence ID" value="ACJ75263"/>
        <property type="gene ID" value="THA_801"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_801"/>
    <dbReference type="eggNOG" id="COG1536">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047835_1_1_0"/>
    <dbReference type="OMA" id="QVRPFEF"/>
    <dbReference type="OrthoDB" id="847822at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000090">
        <property type="entry name" value="Flg_Motor_Flig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023087">
        <property type="entry name" value="Flg_Motor_Flig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011002">
        <property type="entry name" value="FliG_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032779">
        <property type="entry name" value="FliG_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028263">
        <property type="entry name" value="FliG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30534">
        <property type="entry name" value="PTHR30534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01706">
        <property type="entry name" value="FliG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14841">
        <property type="entry name" value="FliG_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14842">
        <property type="entry name" value="FliG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003161">
        <property type="entry name" value="FliG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00954">
        <property type="entry name" value="FLGMOTORFLIG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48029">
        <property type="entry name" value="SSF48029"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00207">
        <property type="entry name" value="fliG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="13" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FliG_N" evidence="12">
        <location>
            <begin position="8"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="FliG_M" evidence="11">
        <location>
            <begin position="117"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="FliG_C" evidence="10">
        <location>
            <begin position="220"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ75263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="336" mass="37651" checksum="EEB79AD37FD57372" modified="2009-02-10" version="1">MAEKTSIPGKRKAAILLVLMGPENAANVLKNLDESDVEQLTIEIANLGNVKDDEKQEVLQEFKELSKAREMLLSGGIEYAKEMLIKAFGPERAMKVIERLVSNLQVKPFEFMRLADPMQVVNFLQSEHPQTIALVLSFLEPPLAAKVLSALPEKIQAEVVKRIALLDRASPDVVREIEKNLEKKFSGLGVQTLSQVGGIDTAAEIINNIDRTTEKSIMEKLGYESPELAEEIRRRLFVFEDLLKLDDRSVQLVLREVDTRDLAVALKGASEELKEKIFNNMSKRAAQLLKDELEFMGPVRIKDVEEAQQKIINVVRRLEEAGEIVIARGGGEELIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGT0</accession>
    <name>B7IGT0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_832</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579822.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_832"/>
    <dbReference type="EnsemblBacteria" id="ACJ75294">
        <property type="protein sequence ID" value="ACJ75294"/>
        <property type="gene ID" value="THA_832"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_832"/>
    <dbReference type="eggNOG" id="COG2211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027408_6_3_0"/>
    <dbReference type="OMA" id="DIIDYGE"/>
    <dbReference type="OrthoDB" id="1249292at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039672">
        <property type="entry name" value="MFS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11328">
        <property type="entry name" value="PTHR11328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="341"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="448" mass="50746" checksum="0F8B86F4D5B02C44" modified="2009-02-10" version="1">MVEKLPFWKKIIYALGQLGWSLTSFAIANVLVYFYMPPEGVNFPEFIKRGPVFLGLTIVGLALGVSRLFDAVTDPLVATLSDRNRMKLGRRRGFLAISVLPFAIFSFLPFFPPSSDHTINTIWLFLTVILFYWFMTMYVTPYFAWMSELGHTPDERLLLSTLISITWAIGYVLGTQIYLFQTILENKGLTPLKAFQTITLIYGLVGFVFMLLPVIFIDENKYCEKHTVKEKGLESIINAFKERNFRLFVLQDFIYWFGLTGISLGLVYYITVLLKLPKEHASQVQLLMFLLSFIFYIPVNFLAKKFGKKNLLIIGFIVFSLDFAVAAMLGKLSFSPIMQSYIIAVLSSVPLAIFGILPNAVIADIAEAHGKETGNYKAAVFFGARTFMQKMGQTFAGLIYPSLFILNSETIGEFGLRISAIFSLILMVLGTIILVFFNEKKILEILKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGV5</accession>
    <name>B7IGV5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cmr crispr-associated ramp protein, Cmr1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_859"/>
    <dbReference type="EnsemblBacteria" id="ACJ75319">
        <property type="protein sequence ID" value="ACJ75319"/>
        <property type="gene ID" value="THA_859"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_859"/>
    <dbReference type="eggNOG" id="COG1367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_762754_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="322" mass="37828" checksum="494A764A9C287903" modified="2009-02-10" version="1">MIKELKVKFKTLSPLWTGNYRGITNEIRPSSLIGSLRFWLDIICYFSGLQINDLGSFDPEKFQNFILKEGTDFESLNKIFSEFRLSLSSKIFGCNKWEGWIRIKEIKPVGKYCFGNYLNLDNSYRKPYFWGTFEVTFEVEEKIINPIFYPLLYFMELYGFWGGKWSLGYGRLQVVNMEIDGKILNSEQWRKNEFKFKLFGNENIFFLENSEKSDIIKVEEGEEKDFEILLSKNKKINVLFLKNIDGKGYSELIKSLKEIKFNKQNEHKSKYLFGSKSKGSKIFPLITYQIQKDKYEGGLKVGFISIVNFLKGSEKNEKANKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGY3</accession>
    <name>B7IGY3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_888</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75347">
        <property type="protein sequence ID" value="ACJ75347"/>
        <property type="gene ID" value="THA_888"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_888"/>
    <dbReference type="HOGENOM" id="CLU_3334140_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="38" mass="4232" checksum="D7715C5F0E817D04" modified="2009-02-10" version="1">MEEFSSAEFAPILISPLVILPLRNTVLSTNFSYSFLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGZ6</accession>
    <name>B7IGZ6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phospho-N-acetylmuramoyl-pentapeptide-transferase</fullName>
            <ecNumber evidence="7">2.7.8.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">UDP-MurNAc-pentapeptide phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">mraY</name>
        <name evidence="8" type="ordered locus">THA_901</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the initial step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan: transfers peptidoglycan precursor phospho-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide onto the lipid carrier undecaprenyl phosphate, yielding undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide, known as lipid I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine = di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UMP</text>
            <dbReference type="Rhea" id="RHEA:28386"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="ChEBI" id="CHEBI:61386"/>
            <dbReference type="ChEBI" id="CHEBI:61387"/>
            <dbReference type="EC" id="2.7.8.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyltransferase 4 family. MraY subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.13" evidence="7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100763.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_901"/>
    <dbReference type="EnsemblBacteria" id="ACJ75360">
        <property type="protein sequence ID" value="ACJ75360"/>
        <property type="gene ID" value="THA_901"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_901"/>
    <dbReference type="eggNOG" id="COG0472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023982_0_1_0"/>
    <dbReference type="OMA" id="LMSPLHH"/>
    <dbReference type="OrthoDB" id="1151822at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051992">
        <property type="term" value="F:UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine:undecaprenyl-phosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06852">
        <property type="entry name" value="GT_MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00038">
        <property type="entry name" value="MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003524">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018480">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00445">
        <property type="entry name" value="mraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01347">
        <property type="entry name" value="MRAY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01348">
        <property type="entry name" value="MRAY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="288" mass="32445" checksum="3E41FAED0A1E4460" modified="2009-02-10" version="1">MYILNFIITAILIYYYIKLMKKIRVGQYIREEGPDLHNYKSGTPTAAGIVFISTASIFLLLAKAPTIFVLSLILFGFIGFIDDISSILKKNALGLRAYQKSLLQVIFSLLLISFTNVRPIFGIEVSKTTYYIFWTFVIVGASNAVNLTDGLDGLAGWVWITSMVPLMVFIKDYSVLIIISSVLAFLLFNSRPASIFMGDTGSLALGAFLATYAMYYNIETQLVFSSSIFIVETLSVIIQVFSFKVFKKRVFKMSPIHHHFELLGWKEEKIVGVFSAINLLISLSILRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH15</accession>
    <name>B7IH15_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579878.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_920"/>
    <dbReference type="EnsemblBacteria" id="ACJ75379">
        <property type="protein sequence ID" value="ACJ75379"/>
        <property type="gene ID" value="THA_920"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_920"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_324346_0_0_0"/>
    <dbReference type="OMA" id="GWKHISY"/>
    <dbReference type="OrthoDB" id="71885at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="914" mass="102454" checksum="E01385012BA70BDA" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH24</accession>
    <name>B7IH24_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579885.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_929"/>
    <dbReference type="EnsemblBacteria" id="ACJ75388">
        <property type="protein sequence ID" value="ACJ75388"/>
        <property type="gene ID" value="THA_929"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_929"/>
    <dbReference type="eggNOG" id="COG4635">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094839_1_0_0"/>
    <dbReference type="OMA" id="FIVCMAQ"/>
    <dbReference type="OrthoDB" id="1804797at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026816">
        <property type="entry name" value="Flavodoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12724">
        <property type="entry name" value="Flavodoxin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="151" mass="17640" checksum="CD804858648C2055" modified="2009-02-10" version="1">MRVCIIYDTKRRSTELFAKWIKEAFEENSAVVDVFKVNEFNGDFSYDLFVVGSPIYYEKPLKSITDFLQKYSDKLSTKKLALFIVCMAQIFGKAGKAYIEKQYLKALESKVENLMFKSAVFKGWIKKVNLKEKKEVFEWVNSLIEKLEGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH54</accession>
    <name>B7IH54_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Flagellar biosynthetic protein FliR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fliR</name>
        <name evidence="11" type="ordered locus">THA_959</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Role in flagellar biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the FliR/MopE/SpaR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579903.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_959"/>
    <dbReference type="EnsemblBacteria" id="ACJ75418">
        <property type="protein sequence ID" value="ACJ75418"/>
        <property type="gene ID" value="THA_959"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_959"/>
    <dbReference type="eggNOG" id="COG1684">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063626_2_3_0"/>
    <dbReference type="OMA" id="APQFNIF"/>
    <dbReference type="OrthoDB" id="1942709at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006303">
        <property type="entry name" value="FliR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002010">
        <property type="entry name" value="T3SS_IM_R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30065">
        <property type="entry name" value="PTHR30065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01311">
        <property type="entry name" value="Bac_export_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00953">
        <property type="entry name" value="TYPE3IMRPROT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01400">
        <property type="entry name" value="fliR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="5 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="11" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="254" mass="28131" checksum="706BAF3941100919" modified="2009-02-10" version="1">MEIIDFVETQFLNWAMLISRLTGMFLITPLLSAAFVPTTVRAFLVLALSYMSLPQVNPIPLDTPVITIVLSLLNNFLIGFAIGLLAYFIFGAFSIAGELLGIESGFGLATAMDPTMEETPIVGQLIFLLSIFVFISLNGHMVVYQGVVDSIKTFPLYLDKLDFSLVKFIETKFIEMFFIALKIGLPVIGYMFIINVLLGILSRLVPQMNVFMVGIPLKSLLVFVIFLGMVPIWAETSAKLTVLLEKVIQQFLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHA9</accession>
    <name>B7IHA9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1015"/>
    <dbReference type="EnsemblBacteria" id="ACJ75473">
        <property type="protein sequence ID" value="ACJ75473"/>
        <property type="gene ID" value="THA_1015"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1015"/>
    <dbReference type="HOGENOM" id="CLU_3240786_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="43" mass="5217" checksum="6AAD55F1B0B98AB6" modified="2009-02-10" version="1">MLQEVKRMKNYKIIKNKIKIIVLNNNIFAWWWTSGNCPPPLSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHB3</accession>
    <name>B7IHB3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1019</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579937.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1019"/>
    <dbReference type="EnsemblBacteria" id="ACJ75477">
        <property type="protein sequence ID" value="ACJ75477"/>
        <property type="gene ID" value="THA_1019"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1019"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_1_0"/>
    <dbReference type="OMA" id="YYRVVRN"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="85"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="290" mass="31392" checksum="238B82876C47D235" modified="2009-02-10" version="1">MRKKFVSERIGEFIGDLFGTGTGWLTFIGAMILIFFIILAIFSPQIAPYDPTQRVGRALTPPNDQFIFGTDNLGRDVLSRVIYGARIALMIAFIAVAIAAGIGVPLGLISGFIGGPLDRTLTLIMDAIYSFPGLILAIAIAAVLGPGIINISISIAVVYTPTYFRVIRNQVSSVKNELYVEAARALGAKNSEILLKYIFPNVLPSIVVVLSMNLADAIMTEAGLSFLGLGIPPPTPDWGFDLSNGQRFVLSRAWWGLLFPGIAIITVVLGFSMFSEGLNELLNPTIRERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHD5</accession>
    <name>B7IHD5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">cobD</name>
        <name evidence="11" type="ordered locus">THA_1041</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the CobD/CbiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101078.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1041"/>
    <dbReference type="EnsemblBacteria" id="ACJ75499">
        <property type="protein sequence ID" value="ACJ75499"/>
        <property type="gene ID" value="THA_1041"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1041"/>
    <dbReference type="eggNOG" id="COG1270">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054212_0_2_0"/>
    <dbReference type="OMA" id="WGYRNER"/>
    <dbReference type="OrthoDB" id="2029688at2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="PTHR34308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00380">
        <property type="entry name" value="cobD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="280" mass="32395" checksum="C6E1E49B42D95A73" modified="2009-02-10" version="1">MFILLISLLIDITIGELPAFIHPVVYMGYIGKLFEKSKHARFLLGMLALLLEILFWLFLCKTLLSISKCFEIYFLTSTFSIKSLYSHVKRCYEDDVEKLRKNVSYIVSRDVSKLNKEKLYSAALESLSENISDGIVGPLFYYLIFGIYGALLYRVVNTYDALFGYRNERYEWFGKFPARFDDVLNFIPARITALLIALFNFKGAFSYLKKYRRLKINSMYPMSAFAGVLNLGFEKVGVYKLEGKPVEKEDILKGLSLYTKVVFVWIAIVLFTVVLKTKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHE3</accession>
    <name>B7IHE3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4130 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579958.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1050"/>
    <dbReference type="EnsemblBacteria" id="ACJ75507">
        <property type="protein sequence ID" value="ACJ75507"/>
        <property type="gene ID" value="THA_1050"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1050"/>
    <dbReference type="eggNOG" id="COG1573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1427378_0_0_0"/>
    <dbReference type="OrthoDB" id="659732at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025404">
        <property type="entry name" value="DUF4130"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13566">
        <property type="entry name" value="DUF4130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4130" evidence="1">
        <location>
            <begin position="86"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="190" mass="22840" checksum="3D987709B4755354" modified="2009-02-10" version="1">MMVSYDGTFNGLFKLIQFCYKNNIIPDFVLKKERKISILVDLSEIEFTKKFIHDSYIFLPFLSEIKNIESLIIRYVVTKNTFLEKTLRKISKDVLTEFEKIKRKLYFLEHNGVFISSFSSNSNIIDLLFLYFLERLKNEKFIIYDEKRNIVISYNNQTRKVLKENRVNLFVQNYDPTLHLWDIYQKSITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHH1</accession>
    <name>B7IHH1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="4 12">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">hemB</name>
        <name evidence="14" type="ordered locus">THA_1079</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>2 5-aminolevulinate = H(+) + 2 H2O + porphobilinogen</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="4 12"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579985.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1079"/>
    <dbReference type="EnsemblBacteria" id="ACJ75535">
        <property type="protein sequence ID" value="ACJ75535"/>
        <property type="gene ID" value="THA_1079"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1079"/>
    <dbReference type="eggNOG" id="COG0113">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035731_0_0_0"/>
    <dbReference type="OMA" id="YQMDYAN"/>
    <dbReference type="OrthoDB" id="677575at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="PTHR11458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ75535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="318" mass="36030" checksum="0ECA1661BBB3B22B" modified="2009-02-10" version="1">MIKRPRRLRKNEIIRSLVREYKVCVDDLVYPLFIKDGYKLKEEIDSMPGIFRYSIDTAIEEIKEARSYGIKSFILFGVPEEKNLECALSGIIPKALRQIKREIEDIVLIADVCMCGYTKEGHCGIVKDGVILNDESLTVLADIALSYAEAGADIVAPSDMMDGRVYEIRKKLDEHKFTDTLIMSYSVKYASSFYGPFRDAAHSAPAFGDRKTYQMDYANKREALMEIELDIEEGADIVMVKPALSYLDIIHLAKEKFNLPVAAYNVSGEYSMVKAASKMGWINEKDIVMEILTSMKRAGADIIITYHAKDVARWITCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHK1</accession>
    <name>B7IHK1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580005.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1112"/>
    <dbReference type="EnsemblBacteria" id="ACJ75565">
        <property type="protein sequence ID" value="ACJ75565"/>
        <property type="gene ID" value="THA_1112"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1112"/>
    <dbReference type="eggNOG" id="COG2954">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1452426_0_0_0"/>
    <dbReference type="OMA" id="VIQWYLE"/>
    <dbReference type="OrthoDB" id="1617215at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="SSF55154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="189" mass="22958" checksum="9878507C65DA9EA3" modified="2009-02-10" version="1">MKIYKKDVIIKNMKVEREKKYLVDEKLFQNLRKKAIYKLGVIQWYLEKCIFIKEEKCRIRYTIDSQGRENWVVAFKSPLFGDYKRLEEEYEVKLEDLSILKEKPVIAKIRYFLLMEKTEVIVDEFIELDYPLKVKYLAEIETDEDFKKFEEMFSLSKAVKDFEEYTNKNLARVSKFSPEEIIRKVQTML</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHN7</accession>
    <name>MURG_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase</fullName>
            <ecNumber evidence="1">2.4.1.227</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murG</name>
        <name type="ordered locus">THA_1149</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-glucosamine = di-trans-octa-cis-undecaprenyl diphospho-[N-acetyl-alpha-D-glucosaminyl-(1-&gt;4)]-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + H(+) + UDP</text>
            <dbReference type="Rhea" id="RHEA:31227"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:61387"/>
            <dbReference type="ChEBI" id="CHEBI:61388"/>
            <dbReference type="EC" id="2.4.1.227"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family. MurG subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.227" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580037.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHN7"/>
    <dbReference type="SMR" id="B7IHN7"/>
    <dbReference type="STRING" id="484019.THA_1149"/>
    <dbReference type="CAZy" id="GT28">
        <property type="family name" value="Glycosyltransferase Family 28"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75601">
        <property type="protein sequence ID" value="ACJ75601"/>
        <property type="gene ID" value="THA_1149"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1149"/>
    <dbReference type="eggNOG" id="COG0707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037404_0_1_0"/>
    <dbReference type="OMA" id="AADMMLC"/>
    <dbReference type="OrthoDB" id="1165736at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051991">
        <property type="term" value="F:UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050511">
        <property type="term" value="F:undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030259">
        <property type="term" value="P:lipid glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00033">
        <property type="entry name" value="MurG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006009">
        <property type="entry name" value="GlcNAc_MurG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000116486" description="UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase">
        <location>
            <begin position="1"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00033"/>
        </source>
    </evidence>
    <sequence length="334" mass="37336" checksum="1211550D821569DE" modified="2009-02-10" version="1">MIKIAVAGGVTGGHLYPALAVLKELEKLTPIDVLYFTVSGKLEERVLKDYNYKKVSLKIQGLKRPVYSIENIKRLFKIFNANNIVLKELKKFKPDIVFVTGGYVSYPVGTAAKKLKIPLYIQEQNVIPGLANIKLSSFAKKVFVSFEESKKYFQRDVVVAGNPILICHKENLNFEKKTILIVGGSGGSEFLNSLACKLSNKLKDYHFILSSGRKEVPCKSENLTILDYIENMSDYYSAVSCAITRGGATTVSELIFFDTPSIIIPWEGSTEAHQIENAKQIEKLGLGYVIREKEVNIDEIANKIIELSNRERKGSPKTNPAILIAKEIKNEVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHQ1</accession>
    <name>B7IHQ1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-ornithine aminomutase S component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580051.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1163"/>
    <dbReference type="EnsemblBacteria" id="ACJ75615">
        <property type="protein sequence ID" value="ACJ75615"/>
        <property type="gene ID" value="THA_1163"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1163"/>
    <dbReference type="eggNOG" id="ENOG5032S0N">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153213_0_0_0"/>
    <dbReference type="OMA" id="GAGHIVW"/>
    <dbReference type="OrthoDB" id="1851520at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015130">
        <property type="entry name" value="Lys-AminoMut_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16552">
        <property type="entry name" value="OAM_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="SSF51703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lys-AminoMut_A" evidence="1">
        <location>
            <begin position="4"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="124" mass="14321" checksum="45AB2B4130AD49D0" modified="2009-02-10" version="1">MKPRKDDFLERSKHLQKMTDDELNQYFWHLVEKVVDPLIELAKTHTSPSVERSVLLRMGFNSIEASKLVDMIFQRGLLGKGAGHIVWKIAKEKNLDIIEAGRALIEGKYWEDVDRIFKGVNNNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHQ6</accession>
    <name>B7IHQ6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DRTGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580053.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1169"/>
    <dbReference type="EnsemblBacteria" id="ACJ75620">
        <property type="protein sequence ID" value="ACJ75620"/>
        <property type="gene ID" value="THA_1169"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1169"/>
    <dbReference type="eggNOG" id="COG4109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140224_1_0_0"/>
    <dbReference type="OMA" id="VTIQTHV"/>
    <dbReference type="OrthoDB" id="1675913at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010766">
        <property type="entry name" value="DRTGG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028979">
        <property type="entry name" value="Ser_kin/Pase_Hpr-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07085">
        <property type="entry name" value="DRTGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75138">
        <property type="entry name" value="SSF75138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DRTGG" evidence="1">
        <location>
            <begin position="32"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="109" mass="11847" checksum="99C0A7DACC104CCA" modified="2009-02-10" version="1">MKLSEIIEKINAEVVYLSEDCDILHGCTGDLLSMVMKNAKSDSIWITVQNHVNIIAVASMVGIKAIVLCEGLEYSPDTIEKAKEEGISLLKSNESSFVVSGKIYSLGII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHT3</accession>
    <name>RS2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsB</name>
        <name type="ordered locus">THA_1197</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580075.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHT3"/>
    <dbReference type="SMR" id="B7IHT3"/>
    <dbReference type="STRING" id="484019.THA_1197"/>
    <dbReference type="EnsemblBacteria" id="ACJ75647">
        <property type="protein sequence ID" value="ACJ75647"/>
        <property type="gene ID" value="THA_1197"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1197"/>
    <dbReference type="eggNOG" id="COG0052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040318_1_2_0"/>
    <dbReference type="OMA" id="SYIDRAY"/>
    <dbReference type="OrthoDB" id="1623045at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_S2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_S2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_S2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_S2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_S2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="PTHR12534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="SSF52313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000119433" description="30S ribosomal protein S2">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="228"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="259" mass="29736" checksum="5D99851F837FE6F5" modified="2009-02-10" version="1">MAVLTMKQLLEAGVHFGHRTQRWNPKMKEYIFGARKGIYIIDLQKTSKLLDEAYNFVRDKAAEGGTILFVGTKKQAQQVIKQEAERCGAFYVNHRWLGGLLTNFETIRKRIDKLIELEEMEANGEFDHLPKKEQSRLRRILEKLRKNLGGLKNMTSLPDVIYIVDPRKERNAVYEANLLKIPTVAIVDTNCDPDEIDYIIPGNDDAIRAIQLITSKIADAYLEGREGVSFGSEEAEENNQKEDNEEIFEIEDVDESEEM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHW7</accession>
    <name>KAD_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenylate kinase</fullName>
            <shortName evidence="1">AK</shortName>
            <ecNumber evidence="1">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Adenylate monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">adk</name>
        <name type="ordered locus">THA_1236</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis. Some bacteria have evolved a zinc-coordinating structure that stabilizes the LID domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101476.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHW7"/>
    <dbReference type="SMR" id="B7IHW7"/>
    <dbReference type="STRING" id="484019.THA_1236"/>
    <dbReference type="EnsemblBacteria" id="ACJ75681">
        <property type="protein sequence ID" value="ACJ75681"/>
        <property type="gene ID" value="THA_1236"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1236"/>
    <dbReference type="eggNOG" id="COG0563">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032354_1_2_0"/>
    <dbReference type="OMA" id="FHNRMRV"/>
    <dbReference type="OrthoDB" id="1491686at2"/>
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        <property type="reaction ID" value="UER00649"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
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        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="PTHR23359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000191172" description="Adenylate kinase">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMP" evidence="1">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="1">
        <location>
            <begin position="126"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="136"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00235"/>
        </source>
    </evidence>
    <sequence length="214" mass="24381" checksum="1BEC212904C9DA3F" modified="2009-02-10" version="1">MNMVFLGPPGAGKGTYAKRLIEMLNIPHISTGDMFREAVASKSELGKKVEEILKRGDLVPDDLTNSIVKDRLSKEDCKNGFILDGFPRTVAQAKALDEIMRSLGKDLDYVIYFEVDEEEVVKRISNRRICSNCGKIYNLITLPPKVDGKCDVCGGTLYQREDDKEEVVRKRYRVYMENTYPVIEYYQKSNKLFTVNGALDVDSVIKEVLNIIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHW9</accession>
    <name>B7IHW9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor IF-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 5" type="primary">infA</name>
        <name evidence="5" type="ordered locus">THA_1238</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IF-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1238"/>
    <dbReference type="EnsemblBacteria" id="ACJ75683">
        <property type="protein sequence ID" value="ACJ75683"/>
        <property type="gene ID" value="THA_1238"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1238"/>
    <dbReference type="eggNOG" id="COG0361">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151267_1_0_0"/>
    <dbReference type="OMA" id="AHVSGKM"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04451">
        <property type="entry name" value="S1_IF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00075">
        <property type="entry name" value="IF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004368">
        <property type="entry name" value="TIF_IF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33370">
        <property type="entry name" value="PTHR33370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00008">
        <property type="entry name" value="infA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S1-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="65" mass="7425" checksum="12D68044C9A07312" modified="2009-02-10" version="1">MEGTIVEALPNAMFRVELENGHKILAHISGKMRKNFIRLVPGDKVVVELTIYDLTKGRIVYRKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHY6</accession>
    <name>B7IHY6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75700">
        <property type="protein sequence ID" value="ACJ75700"/>
        <property type="gene ID" value="THA_1255"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1255"/>
    <dbReference type="HOGENOM" id="CLU_3277928_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="41" mass="4501" checksum="0E5C747716688711" modified="2009-02-10" version="1">MLKTYAIALPESGLILFQSLIGMLKTMNINIDGFGIKSFNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMT0</accession>
    <name>C0QMT0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase repeat family IS605</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_2004</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2004"/>
    <dbReference type="EnsemblBacteria" id="ACN29497">
        <property type="protein sequence ID" value="ACN29497"/>
        <property type="gene ID" value="THA_2004"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2004"/>
    <dbReference type="eggNOG" id="COG0675">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_9_1_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_IS605" evidence="6">
        <location>
            <begin position="12"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="7">
        <location>
            <begin position="99"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="12"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACN29497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="203" mass="23146" checksum="0904DDDECBB39B84" modified="2009-05-05" version="1">MSTGEKYSLPDLSKYENKLKRLHRQLSRKQKGSKNREKARLRLAKAYEKIINIKNDWIHKVTHKIVNENQVGKIVVEDININGMMKNSKLSKYIHMQSWGKFITILTYKAKNLGIEVIKADRFFQSSQTCSVCGYVNKEVKDLKIRKWVCPICKAEHDRDINAAKNLAKYGLTIPVGRGPFEFTPVDSALAAEPAKGLRAITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMW4</accession>
    <name>C0QMW4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_2038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2038"/>
    <dbReference type="EnsemblBacteria" id="ACN29531">
        <property type="protein sequence ID" value="ACN29531"/>
        <property type="gene ID" value="THA_2038"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2038"/>
    <dbReference type="eggNOG" id="COG3547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036902_4_1_0"/>
    <dbReference type="OMA" id="YYTRKVE"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transposase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEDD_Tnp_IS110" evidence="2">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="225"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACN29531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="386" mass="45004" checksum="83977DEE5F2996FF" modified="2009-05-05" version="1">MDFHGIDLIKPFKINNTMDGIKMLEEKIKSVKEENKLNKVVLGMEPSGHYWKVLAWQMKISKEIDYLVGVNPYHVKKSKEFDDNSPGKSDRKDAGVIARLIRDGRYFDIYLPEDIYGELRVITTTREQLVNKRKNAKNMVISVMDEYFPEYEKIYKNIFSEGSIKLLKTHPFPKEIIEVGIEEIEKVLKKSTNGKDWKSRAQKIYESAKKSIGVREGQKSAKIKLRMLLEEIEFLTSQIEELEKEMEELVEETEEGEYIKSVPGIGPIMTAVILGEIGDINRFKSWKEIRKLAGLNLYEISSGEHKGKTRITKRGRPLLRKIIYLMAQTTLKHNHEIRKKYEQLRRREKNPLKVVQALIAIGLKMIRIVFKLAKSKIKYEPEKVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICK3</accession>
    <name>B7ICK3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase, PP-loop superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580119.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1285"/>
    <dbReference type="EnsemblBacteria" id="ACJ75730">
        <property type="protein sequence ID" value="ACJ75730"/>
        <property type="gene ID" value="THA_1285"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1285"/>
    <dbReference type="eggNOG" id="COG0037">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026481_1_1_0"/>
    <dbReference type="OMA" id="IAMDPGY"/>
    <dbReference type="OrthoDB" id="1051352at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP_bind_3" evidence="2">
        <location>
            <begin position="43"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="289" mass="34088" checksum="5094E9CA0E00E03C" modified="2009-02-10" version="1">MRCQHCKKQAILKSPRLCEDHFVEYFERKIGKFLEKYKIKNKKLLVAISGGKDSVVVSYVLSKLKEKFNLQIEYFFIRLGIPTFTEKSEEIATRVTESINYPLTIYDLKKEEGFTIMDVKYKPCSYCGMIRRYILNKYAFENDFDYVVLGHNLDDEVFFIFNNMFNKNITQLQRTGPLTQTIKEKKLVGRIKPLYFLTEEEILLYAKLKNLPHIGCACPNSLESTQKKFKKDIKFSRDQKLNIVYSILEMKKYLPEEKVELKFCEKCGYPTTSDTCKFCRTKEKILSTL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICN6</accession>
    <name>RUVC_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Crossover junction endodeoxyribonuclease RuvC</fullName>
            <ecNumber evidence="1">3.1.21.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Holliday junction nuclease RuvC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Holliday junction resolvase RuvC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ruvC</name>
        <name type="ordered locus">THA_1318</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvC family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101643.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ICN6"/>
    <dbReference type="SMR" id="B7ICN6"/>
    <dbReference type="STRING" id="484019.THA_1318"/>
    <dbReference type="EnsemblBacteria" id="ACJ75763">
        <property type="protein sequence ID" value="ACJ75763"/>
        <property type="gene ID" value="THA_1318"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1318"/>
    <dbReference type="eggNOG" id="COG0817">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091257_3_1_0"/>
    <dbReference type="OMA" id="AICHIWR"/>
    <dbReference type="OrthoDB" id="1815080at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16962">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00034">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020563">
        <property type="entry name" value="X-over_junc_endoDNase_Mg_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002176">
        <property type="entry name" value="X-over_junc_endoDNase_RuvC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30194">
        <property type="entry name" value="PTHR30194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02075">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00696">
        <property type="entry name" value="RSOLVASERUVC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00228">
        <property type="entry name" value="ruvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01321">
        <property type="entry name" value="RUVC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000195275" description="Crossover junction endodeoxyribonuclease RuvC">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00034"/>
        </source>
    </evidence>
    <sequence length="157" mass="17639" checksum="BD762C72D8B0E08C" modified="2009-02-10" version="1">MRIIGVDPGYGILGYGILEKNKNKISHVTHGVITTEKDVPMYKRLGYIYDRFVELLEEYSPDACAIESLFFYKNVKTAIHVGEARGVILLASSQRNIPLYEFTPYQVKNNVTGYGRADKKQVQKMVKMLLNLNDIPKPDDAADALAVAWCLAVEIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICQ7</accession>
    <name>B7ICQ7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1339</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1339"/>
    <dbReference type="EnsemblBacteria" id="ACJ75784">
        <property type="protein sequence ID" value="ACJ75784"/>
        <property type="gene ID" value="THA_1339"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1339"/>
    <dbReference type="HOGENOM" id="CLU_3012810_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="56" mass="6811" checksum="EC4A58DB85E8E59F" modified="2009-02-10" version="1">MNDRDIKNMYKNVDITLVRETLKYIEPMIMFSKKAVLEYLIENIFFFPFSKSKLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICR7</accession>
    <name>B7ICR7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Galactosyltransferase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580166.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1349"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75794">
        <property type="protein sequence ID" value="ACJ75794"/>
        <property type="gene ID" value="THA_1349"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1349"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046858_0_0_0"/>
    <dbReference type="OMA" id="NEMAVEI"/>
    <dbReference type="OrthoDB" id="444026at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="2">
        <location>
            <begin position="281"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="453" mass="53873" checksum="6DC0A01CAD0D2741" modified="2009-02-10" version="1">MEEKELFSQIQNLISNQNFKEAKNLAEKIKDETEKENVLGIIYYYEKNLDESTKHFEKALKFNPIHSDALFNYSKVLFEMENYFESWRYLTRIHEKNWQVFDLLGDTQLKQDNPAMALYYYKKAAELSNIPEMNEKYKVFKNKFKKDVNLAIFCLPGLDNFIKDIAEILSNIYNVILVVSTDRRQIQEAYNWADIVWLEWANEMAVEITNKLPKMNKNIICRLHSYEALLDFPEKINWNNTDTLILVSDHIEVILKNYHSEIYSKHIKNKITIIPNGVNLNKFKFSIKKPGFDLAVVANINHKKAPEMWLQVIHLLKKIDERYTLHIAGKFQDIRYANYFNYFIKDSGLEKNVKLYGYINDINEFLEDKNYILSTSIHEGHPYNIIEAMARGIKPLIHNYAGSKKQWPNELIYNYIFEIPDLLSNNYNSNEYRKFVETEWSLERQINNIFKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICT6</accession>
    <name>B7ICT6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Beta sliding clamp</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">dnaN</name>
        <name evidence="13" type="ordered locus">THA_1368</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a ring-shaped head-to-tail homodimer around DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the beta sliding clamp family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580184.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1368"/>
    <dbReference type="EnsemblBacteria" id="ACJ75813">
        <property type="protein sequence ID" value="ACJ75813"/>
        <property type="gene ID" value="THA_1368"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1368"/>
    <dbReference type="eggNOG" id="COG0592">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038149_4_1_0"/>
    <dbReference type="OMA" id="YLIMPVR"/>
    <dbReference type="OrthoDB" id="1040142at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00140">
        <property type="entry name" value="beta_clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001001">
        <property type="entry name" value="DNA_polIII_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022635">
        <property type="entry name" value="DNA_polIII_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022637">
        <property type="entry name" value="DNA_polIII_beta_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022634">
        <property type="entry name" value="DNA_polIII_beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30478">
        <property type="entry name" value="PTHR30478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00712">
        <property type="entry name" value="DNA_pol3_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02767">
        <property type="entry name" value="DNA_pol3_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02768">
        <property type="entry name" value="DNA_pol3_beta_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000804">
        <property type="entry name" value="DNA_pol_III_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00480">
        <property type="entry name" value="POL3Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00663">
        <property type="entry name" value="dnan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="5 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol3_beta" evidence="10">
        <location>
            <begin position="2"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_2" evidence="11">
        <location>
            <begin position="131"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_3" evidence="12">
        <location>
            <begin position="246"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ75813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="367" mass="41653" checksum="E2EAC9ED553C9E67" modified="2009-02-10" version="1">MLKLAIQRSELEKKISVAAQAVGSKTVDPILQCLLFKVEDNSLNIYSTDMQTFVVSKLNVPEVEGSGQFAVDAKLLEEIVKNVDNDQIILNYDNGKLNIKSGKSSFNIATYSEPEKFPSIELENTGIKFEIETSILQEMIDRVIFCASTESAMRALNGVYWELKDNYLRLVASDGYRLALVEQKITVNSDIDFILSLKSMKELENLIKETDEPVLTINFDHKKLAVNAGDVTVIMRVVEEVFPDYKRVLPKAFKSRIVFNKDQFLESLKRTMIIAKRGTDKVQLKALDDTLELSSQSPEYGEVIEEIPIEKEGEDITINFNPRFLNEAVKKIEEEEVVFNFVDELSPMQINSKNVGEYLYIVLPVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICU4</accession>
    <name>B7ICU4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1376</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580190.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1376"/>
    <dbReference type="EnsemblBacteria" id="ACJ75821">
        <property type="protein sequence ID" value="ACJ75821"/>
        <property type="gene ID" value="THA_1376"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1376"/>
    <dbReference type="eggNOG" id="ENOG5033ZGJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_733085_0_0_0"/>
    <dbReference type="OMA" id="SWENWYK"/>
    <dbReference type="OrthoDB" id="1350782at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="357" mass="41886" checksum="D0B766AEF6E7726E" modified="2009-02-10" version="1">MKKIFLWIFFILPLFLFSEVVLIYPNYGVLVKQIKNGEVIPSTWEVLGVESSWYVKTYEYVEKVKLVEDEDFVLKDCELRDGIFISKDGKKYKFINDRLFEIVQENNEIRRIFIDSSPSTATFTIDGGFQFFYILKENEMYQFLKLYADIEEGFVIVVSEPESSYYTTSTRAMLYEKEVYNVQGKKIFTLGNIKDLKKGKTILMDKFSVERKDYNFVSINGFNSVDWQPCQRVVYVKSQKQIPSGQVQIWSSLSGKEVPIDTTIIKDVDEETEIFLGPSWENWYKWSIDSSVAFANRRKISATLYLKGRGEYKIRINGKNINNLKTNAEIVEKKQDYVILLSHNTQTIHIEYDEDRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICW6</accession>
    <name>B7ICW6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Spermidine/putrescine ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1399</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101788.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1399"/>
    <dbReference type="EnsemblBacteria" id="ACJ75843">
        <property type="protein sequence ID" value="ACJ75843"/>
        <property type="gene ID" value="THA_1399"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1399"/>
    <dbReference type="eggNOG" id="COG1177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_3_0_0"/>
    <dbReference type="OMA" id="FPMFVWG"/>
    <dbReference type="OrthoDB" id="1264541at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="60"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="256" mass="28850" checksum="5B05E361C021D74A" modified="2009-02-10" version="1">MKPGALSKTITIFTFVFFYVPIFIVILFSFNNSKSPIWTGFSLHWYKELFFDSYDVWRAFFNSILVAVSSSLVATALGTLAAIGLYWEKFRGKNTIWIISYLPLIIPDIIIGVSLLIFFVMIKIRLSLLTIFIAHTTFSLSYAMMIVYSRLQDFEYSIVEAAYDLGATKSVALYKVIIPVAFPGILAAFFMSFTLSIDDFVITFFVAGPGSTTLPLKIYSMIRFGISPTINAISTLLLVGTIAISLTMKKYLKYIF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICX1</accession>
    <name>SPEH_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-adenosylmethionine decarboxylase proenzyme</fullName>
            <shortName evidence="1">AdoMetDC</shortName>
            <shortName evidence="1">SAMDC</shortName>
            <ecNumber evidence="1">4.1.1.50</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="1">S-adenosylmethionine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">S-adenosylmethionine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">speH</name>
        <name type="ordered locus">THA_1404</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + S-adenosyl-L-methionine = CO2 + S-adenosyl 3-(methylsulfanyl)propylamine</text>
            <dbReference type="Rhea" id="RHEA:15981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.1.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="1">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic AdoMetDC family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.50" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101797.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ICX1"/>
    <dbReference type="SMR" id="B7ICX1"/>
    <dbReference type="STRING" id="484019.THA_1404"/>
    <dbReference type="EnsemblBacteria" id="ACJ75848">
        <property type="protein sequence ID" value="ACJ75848"/>
        <property type="gene ID" value="THA_1404"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1404"/>
    <dbReference type="eggNOG" id="COG1586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125470_2_3_0"/>
    <dbReference type="OMA" id="HTWPEKG"/>
    <dbReference type="OrthoDB" id="1613081at2"/>
    <dbReference type="UniPathway" id="UPA00331">
        <property type="reaction ID" value="UER00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006557">
        <property type="term" value="P:S-adenosylmethioninamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00464">
        <property type="entry name" value="AdoMetDC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042286">
        <property type="entry name" value="AdoMetDC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003826">
        <property type="entry name" value="AdoMetDC_fam_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042284">
        <property type="entry name" value="AdoMetDC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017716">
        <property type="entry name" value="S-AdoMet_deCOase_pro-enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866">
        <property type="entry name" value="PTHR33866"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02675">
        <property type="entry name" value="AdoMet_dc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="SSF56276"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03330">
        <property type="entry name" value="SAM_DCase_Bsu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_1000125463" description="S-adenosylmethionine decarboxylase beta chain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_1000125464" description="S-adenosylmethionine decarboxylase alpha chain" evidence="1">
        <location>
            <begin position="63"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="1">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis" evidence="1">
        <location>
            <begin position="62"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00464"/>
        </source>
    </evidence>
    <sequence length="130" mass="14832" checksum="8755319D8CB611F0" modified="2009-02-10" version="1" precursor="true">MKSLGRHIIAEFYDCDKEILDNVEKIEQSMKNAAYETGATLVNSSFHRFLPYGVSGVVVISESHLTIHTWPEYGYAAVDLFTCGDDVDPWKAFTYLKEVLKSQRVHVVEHLRGKYDEIGIPENSPHKMEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID19</accession>
    <name>B7ID19_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1453</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580232.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1453"/>
    <dbReference type="EnsemblBacteria" id="ACJ75896">
        <property type="protein sequence ID" value="ACJ75896"/>
        <property type="gene ID" value="THA_1453"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1453"/>
    <dbReference type="eggNOG" id="ENOG50336XU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1110126_0_0_0"/>
    <dbReference type="OMA" id="MEYYICE"/>
    <dbReference type="OrthoDB" id="1214620at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="227" mass="26435" checksum="4EB8B77CC11B55B4" modified="2009-02-10" version="1">MYYLCEIEDDNDLIKIALPKEKVIGISDKVVKSTKTCDFALHKNKLYPILTHADLKNPKLKFFLIFKDFAFGITRLVKETKSTPIEIIKNELYTGLIKDIEEYFIYNIEKLKPAQKVRYLDRNDNNKFDEGKFNYVIVDRKYAISINNILTILLSKEIVKYPMEKFIGFVEYKKIIPVKQVKEGKYVIITKDIAYQCSDIETANGKVLEGKDEKVLESNIGKFPILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID24</accession>
    <name>B7ID24_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1458</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580237.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1458"/>
    <dbReference type="EnsemblBacteria" id="ACJ75901">
        <property type="protein sequence ID" value="ACJ75901"/>
        <property type="gene ID" value="THA_1458"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1458"/>
    <dbReference type="eggNOG" id="ENOG5032RQK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114103_0_0_0"/>
    <dbReference type="OMA" id="DPGYIHH"/>
    <dbReference type="OrthoDB" id="2010192at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025529">
        <property type="entry name" value="DUF4416"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14385">
        <property type="entry name" value="DUF4416"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="179" mass="21862" checksum="23F9ECD841AA91CA" modified="2009-02-10" version="1">MGKVRRVDLVNLVMFFFSSQVEYWLSEVENELIEKFGPIDYKSDILDFEKYTLYYNKEMGEGVKGILLSFERLIHPYQLADIKNMTNEIEQRYAVKGNRRFNIDPGYIHHMQFVLATTKMWPHRIYIGKGIYAETTLMYINGRWKDYDFTYPNYKEEEYKRELEKIRLKYLEKRKKFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID30</accession>
    <name>B7ID30_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease Y</fullName>
            <shortName evidence="5">RNase Y</shortName>
            <ecNumber evidence="5">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rny</name>
        <name evidence="8" type="ordered locus">THA_1464</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Endoribonuclease that initiates mRNA decay.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RNase Y family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580240.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1464"/>
    <dbReference type="EnsemblBacteria" id="ACJ75907">
        <property type="protein sequence ID" value="ACJ75907"/>
        <property type="gene ID" value="THA_1464"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1464"/>
    <dbReference type="eggNOG" id="COG1418">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028328_1_0_0"/>
    <dbReference type="OMA" id="PHAILGM"/>
    <dbReference type="OrthoDB" id="1012190at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00335">
        <property type="entry name" value="RNase_Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017705">
        <property type="entry name" value="Ribonuclease_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022711">
        <property type="entry name" value="RNase_Y_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF15">
        <property type="entry name" value="PTHR12826:SF15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12072">
        <property type="entry name" value="RNase_Y_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03319">
        <property type="entry name" value="RNase_Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HD" evidence="7">
        <location>
            <begin position="326"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="27"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="510" mass="58357" checksum="745D780616035363" modified="2009-02-10" version="1">MLIYILTFVGVFVGALVGYVVAKRKIEKDLLLAKKDAEHIIKEAEKEASEIKKKAVIESREELHKLREDWEKEKKAREEELKYAEERLIKREEMVSKREELLDKKENYIEELRKELEEKQRELIEKEKEIEESFEKLAGITPEQAREIVLNEAREKYEYEIAKVYSQIKTRYEEDAEKYAKKVIADAIQRYAPEYSGEVTVSTVMLPNDDMKGRLIGREGRNIRAFEKVTGVDLIIDDTPEMVTVSCFNPLRREIARRTIEKLVADGRIHPTRIEEMYEKAKDEVERIIKEAGQEATFVTGVGGLHPEIVKLLGRLKFRTSYGQNVLNHSIEVALIAGLLASELGLNVEMAKRGGLLHDIGKALDHEVEGSHTIIGAEILKRYGEKKEVINMIMAHHGEEEPMTPEAVIVAAADALSAARPGARREDVENYIKRLIKLEEIAKSFKYVENAYAIQAGREVRVIVQPDKVDDVLADKLSHDIAMKIEEELQYPGVLKVVVIREKRSVAYAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID78</accession>
    <name>B7ID78_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Peptidase, M50 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">THA_1514</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102041.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1514"/>
    <dbReference type="EnsemblBacteria" id="ACJ75955">
        <property type="protein sequence ID" value="ACJ75955"/>
        <property type="gene ID" value="THA_1514"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1514"/>
    <dbReference type="eggNOG" id="COG1994">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086979_1_1_0"/>
    <dbReference type="OMA" id="HEIMHGL"/>
    <dbReference type="OrthoDB" id="1401629at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06158">
        <property type="entry name" value="S2P-M50_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044537">
        <property type="entry name" value="S2P-M50-like"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ACJ75955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="206" mass="23423" checksum="55EEB90EF47F790E" modified="2009-02-10" version="1">MIVTIEKVIINLLTGFLAVILVVYPRELLKAHLVVLAGDSTPKKLKRTSLNPFVHLDPIGTTAFILFDFGWSRPIPVVPMKNRRIRKVLIFSSLIGSILGVGLFILYGIIAKEVDNKFVYVTFYKACKWSLTYAIFSLFPIPPLDGSKLLGAFLPDEYWDWYIKYEVYGILFLLGMLLIWILPMIMEPFVNFINHITDILIIGKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID88</accession>
    <name>B7ID88_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102054.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1524"/>
    <dbReference type="EnsemblBacteria" id="ACJ75965">
        <property type="protein sequence ID" value="ACJ75965"/>
        <property type="gene ID" value="THA_1524"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1524"/>
    <dbReference type="eggNOG" id="COG0760">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030639_0_0_0"/>
    <dbReference type="OMA" id="AHGAIIW"/>
    <dbReference type="OrthoDB" id="744756at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="542" mass="62178" checksum="CE9C0650319EF2CF" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDE6</accession>
    <name>B7IDE6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peroxiredoxin</fullName>
            <ecNumber evidence="7">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Thioredoxin peroxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Thioredoxin-dependent peroxiredoxin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_1585</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[thioredoxin]-dithiol + a hydroperoxide = [thioredoxin]-disulfide + an alcohol + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodecamer. Pentamer of dimers that assemble into a ring structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. Although the primary sequence of this enzyme is similar to those of the 1-Cys Prx6 enzymes, its catalytic properties resemble those of the typical 2-Cys Prxs and C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102328.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1585"/>
    <dbReference type="EnsemblBacteria" id="ACJ76023">
        <property type="protein sequence ID" value="ACJ76023"/>
        <property type="gene ID" value="THA_1585"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1585"/>
    <dbReference type="eggNOG" id="COG0450">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_4_4_0"/>
    <dbReference type="OMA" id="MIDYQDT"/>
    <dbReference type="OrthoDB" id="892697at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00401">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022915">
        <property type="entry name" value="Peroxiredoxin_TDXH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 7 10" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="9">
        <location>
            <begin position="4"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="7 8">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Interchain (with Cys-207); in linked form" evidence="7">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Alternate" evidence="7">
        <location>
            <begin position="201"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain (with Cys-46); in linked form" evidence="7">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ76023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="214" mass="24525" checksum="7076F09FAA93F01D" modified="2009-02-10" version="1">MGIPLIGERIPEFEAVTTKGVIKFPDDFKGKWVVLFSHPADFTPVCTTEFVAFQKRFDEFQKLNTELVGLSIDQVFSHIKWVEWIKDKLGVEIKFPVIADDRGKIAEMLGMIHPAKGTNTVRAVFIIDPKGILRAVLYYPQELGRNMDEILRMVKGFQVIDENGVALPANWPNNELVNDEAIVPPASTVKDAEQRTKEYECFDWWFCHKKLNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDG6</accession>
    <name>B7IDG6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Triosephosphate isomerase</fullName>
            <shortName evidence="3">TIM</shortName>
            <shortName evidence="3">TPI</shortName>
            <ecNumber evidence="3 4">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">tpiA</name>
        <name evidence="5" type="ordered locus">THA_1605</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="3 4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580291.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1605"/>
    <dbReference type="EnsemblBacteria" id="ACJ76043">
        <property type="protein sequence ID" value="ACJ76043"/>
        <property type="gene ID" value="THA_1605"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1605"/>
    <dbReference type="eggNOG" id="COG0149">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024251_2_3_0"/>
    <dbReference type="OMA" id="QEVCGAI"/>
    <dbReference type="OrthoDB" id="1266295at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="PTHR21139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="SSF51351"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3 4" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="3 4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="221"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="active site" description="Electrophile" evidence="3">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="240" mass="26808" checksum="4085925C93ECC185" modified="2009-02-10" version="1">MHKTPTESAHFASILLNAFHGEKEFDIIVAPTFISIPAVYEILRDSNIKVAAQNMYFEDEGAYTGEISWKMLKESGVEYVIIGHSERRNIFGETDEMINKKIKKAIEVGLTPIFCVGEKLEEREEGLTFAVVETQIRKGLKNIEDIENVIIAYEPVWAIGTGKVATPAQAQEVHSFIRSLISKMYSKERAEKVRILYGGSIKPNNFFSLIVQPDIDGGLVGGASLKESFIELGKILKQVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDJ3</accession>
    <name>B7IDJ3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flg_hook domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1632</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580311.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1632"/>
    <dbReference type="EnsemblBacteria" id="ACJ76070">
        <property type="protein sequence ID" value="ACJ76070"/>
        <property type="gene ID" value="THA_1632"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1632"/>
    <dbReference type="eggNOG" id="COG3144">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_369574_0_0_0"/>
    <dbReference type="OMA" id="PRVEQAN"/>
    <dbReference type="OrthoDB" id="2132812at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021136">
        <property type="entry name" value="Flagellar_hook_control-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038610">
        <property type="entry name" value="FliK-like_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02120">
        <property type="entry name" value="Flg_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_hook" evidence="2">
        <location>
            <begin position="649"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="717"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="753" mass="86841" checksum="158C53AEB7F26A7B" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDJ6</accession>
    <name>B7IDJ6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1635"/>
    <dbReference type="EnsemblBacteria" id="ACJ76073">
        <property type="protein sequence ID" value="ACJ76073"/>
        <property type="gene ID" value="THA_1635"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1635"/>
    <dbReference type="eggNOG" id="COG1582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173020_1_0_0"/>
    <dbReference type="OMA" id="HQIESIE"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009384">
        <property type="entry name" value="SwrD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39185">
        <property type="entry name" value="PTHR39185"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06289">
        <property type="entry name" value="FlbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="73" mass="8471" checksum="8325703418AC3C48" modified="2009-02-10" version="1">MIFIILLTSLSGQQFYLNADYIEKVEELPDTTITLYNGKKYIVSEDAKTVIERIIEYKKKILNIPPLFKDGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDK0</accession>
    <name>B7IDK0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar motor switch protein FliM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fliM</name>
        <name evidence="11" type="ordered locus">THA_1639</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FliM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580317.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1639"/>
    <dbReference type="EnsemblBacteria" id="ACJ76077">
        <property type="protein sequence ID" value="ACJ76077"/>
        <property type="gene ID" value="THA_1639"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1639"/>
    <dbReference type="eggNOG" id="COG1868">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052646_0_0_0"/>
    <dbReference type="OMA" id="NPQFTQI"/>
    <dbReference type="OrthoDB" id="1748746at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17908">
        <property type="entry name" value="FliM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001689">
        <property type="entry name" value="Flag_FliM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001543">
        <property type="entry name" value="FliN-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036429">
        <property type="entry name" value="SpoA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02154">
        <property type="entry name" value="FliM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01052">
        <property type="entry name" value="FliMN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002888">
        <property type="entry name" value="FliM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00955">
        <property type="entry name" value="FLGMOTORFLIM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101801">
        <property type="entry name" value="SSF101801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="SSF103039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01397">
        <property type="entry name" value="fliM_switch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="11" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FliMN_C" evidence="10">
        <location>
            <begin position="254"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ76077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="329" mass="37430" checksum="BC64A5A126FF130E" modified="2009-02-10" version="1">MSDVLSQEEIDRLLQAVNQGEVSIEEVKKEEEEKKVRTYDFLRPRKFSKEQERTLQMLHENFARSLSTYFSGRLRSFVAVDLVGLDQMTYDEFVKSIRNPSYIAVFSAKEFVGSAILDIDLEIFFGVLEILLGGPGMNVEINRPPTDTEIEVMRKEVVNILTNLVQAWSTVYSFTPVIEAIETNPQFVQIVASNEMVLVITLLLKIGNIEGYINICWPSSLVEPIGEKLTTQSWFKTKQKIVSEEDMKALRENLARTIVELSVAVGETNLTLWDILNLEVGDVIRLDTFKEDPLKIKLNGKDKFLGVPGVFKGKYAVKIVEVLDEEEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDK1</accession>
    <name>B7IDK1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Flagellar motor switch phosphatase FliY</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_1640</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FliN/MopA/SpaO family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580318.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1640"/>
    <dbReference type="EnsemblBacteria" id="ACJ76078">
        <property type="protein sequence ID" value="ACJ76078"/>
        <property type="gene ID" value="THA_1640"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1640"/>
    <dbReference type="eggNOG" id="COG1776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1886">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033893_0_0_0"/>
    <dbReference type="OMA" id="MMGGDGK"/>
    <dbReference type="OrthoDB" id="1875967at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007597">
        <property type="entry name" value="CheC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012826">
        <property type="entry name" value="FliN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001543">
        <property type="entry name" value="FliN-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001172">
        <property type="entry name" value="FliN_T3SS_HrcQb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036429">
        <property type="entry name" value="SpoA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04509">
        <property type="entry name" value="CheC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01052">
        <property type="entry name" value="FliMN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00956">
        <property type="entry name" value="FLGMOTORFLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101801">
        <property type="entry name" value="SSF101801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="SSF103039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02480">
        <property type="entry name" value="fliN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="10" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheC" evidence="9">
        <location>
            <begin position="28"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="CheC" evidence="9">
        <location>
            <begin position="125"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="FliMN_C" evidence="8">
        <location>
            <begin position="285"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="225"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ76078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="365" mass="39537" checksum="4F4406EF3EA8F690" modified="2009-02-10" version="1">MGDEQFLSQEELDSLLNQLNEDEDLTEIEKDMFGEIGNIIMGSGTTALSTLLGRKVDITVPTVDLKKISEIKKEVTGKNAVVSIHFKGAIEGLNALIIPQMLVAQIANIMMGGTGEVENEEVDEISLSAVSEAMNQMMGAAATSLSDMLKKPVDITPPTVELIDFDGDNVNFPPIATSDDAVVAKVNFKLVIEGLEPADFFLAMPPEFVKKLYSMIFESEKQEKVESSAAGTSSPVSTQNYAEHSATTTKNVEKNPVSVSPVQFQSFESGGTVKSTGEIPDRIQALLDIPLNVVVELGKTKLTLKQVMELSVGSLIELDKLTGEPVDIIVNGKLIARGEVVVIDENFGVRITEIVTPQERFYTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDP9</accession>
    <name>B7IDP9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH dehydrogenase subunit n</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1689</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1689"/>
    <dbReference type="EnsemblBacteria" id="ACJ76126">
        <property type="protein sequence ID" value="ACJ76126"/>
        <property type="gene ID" value="THA_1689"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1689"/>
    <dbReference type="eggNOG" id="COG0651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_9_2_0"/>
    <dbReference type="OMA" id="AFPVNFW"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="3">
        <location>
            <begin position="62"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="402" mass="43915" checksum="7A64C49776DA74F7" modified="2009-02-10" version="1">MGGWKAPFGIVLVLDSASFYTLLVVNIIFLLISLMPQITDKGLSIILLILLASTNGLILTGDIFNSFVFMEITAAAAYIIASSKKNYYGAYKYLIVGSIAGGFYLLGAIYSYLGTGSLNFADISLNMKESFLPYVALMLFIGLAVESKILPLAGWVPDVYASGSPLTPAILGTGVTFTMIYLLSRIFITVFNGYFLDVIYIFGLLTLILAEIAAFKQEKLLKALAFSSIAQAGLVLSVISLNTYDSLKAGYFHLLNDATAKIILFVIAATVVGSFMKNKTAGISFSIASFSLIGFPLFAGFRSKLLILQSSFESGNYILPAALLFSTIIEAAYLLKWNIHLWYENNEKSEKIPWNIVLITLILSLFIIYIGLFPDTYLKIAENITNGLMNYKAYVTTVLGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDR6</accession>
    <name>B7IDR6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1707</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580364.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1707"/>
    <dbReference type="EnsemblBacteria" id="ACJ76143">
        <property type="protein sequence ID" value="ACJ76143"/>
        <property type="gene ID" value="THA_1707"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1707"/>
    <dbReference type="HOGENOM" id="CLU_1467551_0_0_0"/>
    <dbReference type="OMA" id="YERDYIE"/>
    <dbReference type="OrthoDB" id="1946426at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="176" mass="20767" checksum="F811CAAE5C61FC7F" modified="2009-02-10" version="1">MFNFIENIAGKRDFFIYGTLHFDVYDQNNKNLQSDITLDATILNLEHFFVQIYKPEFLKDIQIEFNSLTKTTYYKYNNYKVVDVSAIDLDYIYEILKTIFSFLSSSLFTIYEDQENLTLIPAGAPFLKRLGLEPTKISVYFDKDSLKKIMFTTDDSTENITITFDKFQILNLENQR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDR9</accession>
    <name>UPP_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">UMP pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UPRTase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">upp</name>
        <name type="ordered locus">THA_1710</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + UMP = 5-phospho-alpha-D-ribose 1-diphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:13017"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit. The magnesium is bound as Mg-PRPP.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by GTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDR9"/>
    <dbReference type="SMR" id="B7IDR9"/>
    <dbReference type="STRING" id="484019.THA_1710"/>
    <dbReference type="EnsemblBacteria" id="ACJ76146">
        <property type="protein sequence ID" value="ACJ76146"/>
        <property type="gene ID" value="THA_1710"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1710"/>
    <dbReference type="eggNOG" id="COG0035">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067096_2_2_0"/>
    <dbReference type="OMA" id="TYATRMP"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00636"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006223">
        <property type="term" value="P:uracil salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01218_B">
        <property type="entry name" value="Upp_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034332">
        <property type="entry name" value="Upp_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005765">
        <property type="entry name" value="Ura_phspho_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01091">
        <property type="entry name" value="upp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0021">Allosteric enzyme</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000139170" description="Uracil phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="130"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="198"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01218"/>
        </source>
    </evidence>
    <sequence length="208" mass="23201" checksum="B333E4C676A44CAC" modified="2009-02-10" version="1">MKINVVDHPLIKHKLTIMRKTDTGPKEFRELLKEITLLIAYEATRHLEIHETEIETPLEKTKGYFIDDKDVVIVPILRAGLGMSDGILQLLPNASVGHIGIYREPHTLEAVEYYAKLPKINENSFVFVLDPMLATGVSSVKALDIVKEHGAKNVILVTLIASPEGTRVVNDKHPDVIIYTASLDRELNSKGYILPGLGDAGDRLFRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDU5</accession>
    <name>B7IDU5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal-sulfur cluster biosynthetic enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102627.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1737"/>
    <dbReference type="EnsemblBacteria" id="ACJ76172">
        <property type="protein sequence ID" value="ACJ76172"/>
        <property type="gene ID" value="THA_1737"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1737"/>
    <dbReference type="eggNOG" id="COG2151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091588_2_2_0"/>
    <dbReference type="OMA" id="PPWGPER"/>
    <dbReference type="OrthoDB" id="1668114at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="1">
        <location>
            <begin position="6"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="101" mass="11398" checksum="51CA004082935FC0" modified="2009-02-10" version="1">MGKITEEMVYNKLKEVIDFEIGLDVVSLGLVYEIKIDENDNVYVLMTMTTPMCPLAGLILQDAETKLREIEGINDVKVELTFDPPWTPDRVDPSVRAQLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDU9</accession>
    <name>B7IDU9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PolyA polymerase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1742</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580381.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1742"/>
    <dbReference type="EnsemblBacteria" id="ACJ76176">
        <property type="protein sequence ID" value="ACJ76176"/>
        <property type="gene ID" value="THA_1742"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1742"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0617">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0618">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015961_5_0_0"/>
    <dbReference type="OMA" id="ILCHTTA"/>
    <dbReference type="OrthoDB" id="2018439at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="311"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="373"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="859" mass="99416" checksum="00948B2A785E9706" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDX0</accession>
    <name>B7IDX0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA methyltransferase, TrmH family, group 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580394.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1766"/>
    <dbReference type="EnsemblBacteria" id="ACJ76197">
        <property type="protein sequence ID" value="ACJ76197"/>
        <property type="gene ID" value="THA_1766"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1766"/>
    <dbReference type="eggNOG" id="COG0566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021322_0_1_0"/>
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    <dbReference type="OrthoDB" id="1422015at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46429">
        <property type="entry name" value="PTHR46429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00186">
        <property type="entry name" value="rRNA_methyl_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_sub_bind" evidence="1">
        <location>
            <begin position="2"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="238" mass="26684" checksum="3B89921D7ABE59E7" modified="2009-02-10" version="1">MKVYGRNILKEILETNTKVRMIYFSDTNSAELTELIEEVKKRRLPFTIANKKVLQRLSNTEKHQGVVVDIGEFEYKDESIIESLEKPFLVILDQLQDPHNFGAIIRTAVASGADAIIIPKNNSVQVTPTVVKVSVGTIFKINIIKTINIARFIKNIKEMGIWVYGADTNGKVYYKTDLRKPIAIVLGNEGAGIRPNVKEKCDALITIPMKNSVESLNVSVSAGILLYEVMRQNENFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDZ4</accession>
    <name>B7IDZ4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76221">
        <property type="protein sequence ID" value="ACJ76221"/>
        <property type="gene ID" value="THA_1790"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1790"/>
    <dbReference type="HOGENOM" id="CLU_217323_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021320">
        <property type="entry name" value="DUF2905"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11146">
        <property type="entry name" value="DUF2905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="37" mass="4185" checksum="5DFCAACF9A068328" modified="2009-02-10" version="1">MPGDIVIKRGNFVLIIPITSMIILSIILTIVMSLFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE01</accession>
    <name>B7IE01_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4234 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1797</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580422.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1797"/>
    <dbReference type="EnsemblBacteria" id="ACJ76228">
        <property type="protein sequence ID" value="ACJ76228"/>
        <property type="gene ID" value="THA_1797"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1797"/>
    <dbReference type="HOGENOM" id="CLU_1824424_0_0_0"/>
    <dbReference type="OMA" id="YENISQM"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025328">
        <property type="entry name" value="DUF4234"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14018">
        <property type="entry name" value="DUF4234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4234" evidence="2">
        <location>
            <begin position="110"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="141" mass="16883" checksum="F14C6D50AA50277E" modified="2009-02-10" version="1">MIISFWGYTFFSILANIFVFFNEPISQILLTISSLFLMFLLFLFGRKIGKKFNVNFMWIFYIALFLALFSLLPLYLTSSYFTYFVAQFMSFLFILLYLKVILSIIKSKKSIFKFIVFSILTLGIYSYFFFYDIIKEIKKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE03</accession>
    <name>B7IE03_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Thiamine biosynthesis protein ThiC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">thiC</name>
        <name evidence="9" type="ordered locus">THA_1799</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580424.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1799"/>
    <dbReference type="EnsemblBacteria" id="ACJ76230">
        <property type="protein sequence ID" value="ACJ76230"/>
        <property type="gene ID" value="THA_1799"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1799"/>
    <dbReference type="eggNOG" id="COG0422">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013181_2_2_0"/>
    <dbReference type="OMA" id="TWELFRD"/>
    <dbReference type="OrthoDB" id="505395at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="PTHR30557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="425" mass="47521" checksum="B359DEF261D06376" modified="2009-02-10" version="1">MTLIEKVKNGEKCSEIEKIAEHENVSVNKIIEGLLNGKIVIPHNKNHTSLKKFIGIGEGLTVKVNANIGTSFGYDDFEYEKEKFNTAKAVGANSVMLLSTWGNLDEQREFIIKNSDIPVGTVPIYDAAVISYQKKKNVVDFSEKDFLKIFEKHAKQGVDFVTLHISITKDIVKKLKSSKRIMKIVSRGGSIIAGWIIKNNLENPFYKYFDEVLDIAREYDVTLSLGDSLRPGNLFDSLDRLQLEEWFIFEELVEKARKKNVQVMLEGPGHVPLDQIETTVNLMKKYGKNSPVFLLGPIVLDIAPGYDHITSAIGAAIAARSGADFICYVTPSEHLSLPNVEDVKKGVIAAKIAAMAADFSRGKFIDENYKLAISRKNLDWQGIKEVSLDKEIVERYLSARPYSGKGCSMCGPFCALKVVEEYLEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE32</accession>
    <name>KCY_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytidylate kinase</fullName>
            <shortName evidence="1">CK</shortName>
            <ecNumber evidence="1">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine monophosphate kinase</fullName>
            <shortName evidence="1">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cmk</name>
        <name type="ordered locus">THA_1828</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + CMP = ADP + CDP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + dCMP = ADP + dCDP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytidylate kinase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580449.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IE32"/>
    <dbReference type="SMR" id="B7IE32"/>
    <dbReference type="STRING" id="484019.THA_1828"/>
    <dbReference type="EnsemblBacteria" id="ACJ76259">
        <property type="protein sequence ID" value="ACJ76259"/>
        <property type="gene ID" value="THA_1828"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1828"/>
    <dbReference type="eggNOG" id="COG0283">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079959_0_2_0"/>
    <dbReference type="OMA" id="RAITWWM"/>
    <dbReference type="OrthoDB" id="776861at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00238">
        <property type="entry name" value="Cytidyl_kinase_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003136">
        <property type="entry name" value="Cytidylate_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02224">
        <property type="entry name" value="Cytidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00017">
        <property type="entry name" value="cmk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000119028" description="Cytidylate kinase">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00238"/>
        </source>
    </evidence>
    <sequence length="222" mass="25127" checksum="D10001549CF9D16B" modified="2009-02-10" version="1">MPCRIAIDGPAGSGKTTVAKILADKLGIYYLDTGAMYRIIGLYLDKKGVENDSDIERELSTIKLEFINGDFYINGKRVGDEIRTPYAGICASKYAKKAIVREFLTRIQQKISKNENIVVEGRDIGTVVIPDAEVKIFLVASAEERAKRRYKELLDKGVKVSYEEVLNEIILRDKQDTEREIAPLRQPDDAVLIDSTKYTIEEVINKILEVVLEKCKLKLQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE86</accession>
    <name>B7IE86_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580469.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1887"/>
    <dbReference type="EnsemblBacteria" id="ACJ76313">
        <property type="protein sequence ID" value="ACJ76313"/>
        <property type="gene ID" value="THA_1887"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1887"/>
    <dbReference type="eggNOG" id="COG4123">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093128_0_0_0"/>
    <dbReference type="OMA" id="SFDVVYF"/>
    <dbReference type="OrthoDB" id="1696461at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008990">
        <property type="term" value="F:rRNA (guanine-N2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007536">
        <property type="entry name" value="16SrRNA_methylTrfase_J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36112">
        <property type="entry name" value="PTHR36112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04445">
        <property type="entry name" value="SAM_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="260" mass="30555" checksum="A5353403F15A898A" modified="2009-02-10" version="1">MRYIVTTSYNANKEIVRLAKNIAEEFNVKYINRNHVSKYLKSGKIDFYYVVDKKGMLKIKWKDGEFFFHQGIAKIRMENIKHGERDYLIESLKPEKTDVVYDATCGLASDAILIANFAKKVVATEGSEHIYRVVKWGLKRYISDEEWINNAMKKIELIHDNYKNFVRNQPDNSFDIVYCDPMFENPIYESNSLNPLRGFAVYEPLSLEDLEHMVRISRKRVVIKTLKKDSLYEKIKNYFSKEFISRKSGIVYGVINKSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE96</accession>
    <name>B7IE96_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM protein, family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1897</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1897"/>
    <dbReference type="EnsemblBacteria" id="ACJ76323">
        <property type="protein sequence ID" value="ACJ76323"/>
        <property type="gene ID" value="THA_1897"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1897"/>
    <dbReference type="eggNOG" id="COG1242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060920_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005911">
        <property type="entry name" value="YhcC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="PTHR11135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF1">
        <property type="entry name" value="PTHR11135:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01212">
        <property type="entry name" value="TIGR01212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="271" mass="31534" checksum="C89ABE73FF1D626F" modified="2009-02-10" version="1">MFCDETGSGFTTFGEFDIKTQIKKMKERYTKKGAKKFIAYFQNYSNTYAPIEMLKKVYLTALDEDIVQISISTRPDCISDEILDLLEELREKIDVSIEMGLQTSNYHTLVKMNRGHSLAEYIYAAKKIKERNFEIISHVILNFPTDDMLDVIETAKILSVLDIDGVKIHSLYVVENTILGKLFNENKIQIGTLESYIERVIRFLEFLSPKITIHRLVADPPNNGTIFGNWGIPKIQIINMIEKRLKEKDTYQGKYFYKWFEPKTNKLSKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IED2</accession>
    <name>B7IED2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1936</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580491.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1936"/>
    <dbReference type="EnsemblBacteria" id="ACJ76359">
        <property type="protein sequence ID" value="ACJ76359"/>
        <property type="gene ID" value="THA_1936"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1936"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_0"/>
    <dbReference type="OMA" id="WAIAWRI"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="76"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="283" mass="32514" checksum="019E95DC461B8588" modified="2009-02-10" version="1">MSNKIKVKTKRALIGYLFFSPWLIGFIVFTAYPFFYSLYLSFFKVRFTVSGVESTFVGLEFYKYAFRGDLTFPINFTNTIINIVLSTPLIVIFALIVSILLNNKIKLRAFFRLLYFLPVVIISGPVVSELVANNASKIVDPGKYFIYQFFTTLPDRISFPFLYMFDNLVLILWFSGVQILFFLAGLQKISPSIYEAAKIDGANSWVIFWKITLPLIRPFILITTIYTIVDLASFANNSVNTSITQHMFDIDKPYSYSAALSWIYFVSVMIIIGIAFLLLRKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IED9</accession>
    <name>B7IED9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dipeptide epimerase</fullName>
            <ecNumber evidence="5">5.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1944</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="4 5">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the mandelate racemase/muconate lactonizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103019.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1944"/>
    <dbReference type="EnsemblBacteria" id="ACJ76366">
        <property type="protein sequence ID" value="ACJ76366"/>
        <property type="gene ID" value="THA_1944"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1944"/>
    <dbReference type="eggNOG" id="COG4948">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_4_1_0"/>
    <dbReference type="OMA" id="MFGCYSD"/>
    <dbReference type="OrthoDB" id="951991at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016855">
        <property type="term" value="F:racemase and epimerase activity, acting on amino acids and derivatives"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03319">
        <property type="entry name" value="L-Ala-DL-Glu_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034603">
        <property type="entry name" value="Dipeptide_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MR_MLE" evidence="6">
        <location>
            <begin position="140"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for (R)-substrate epimerization" evidence="3">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for (S)-substrate epimerization" evidence="3">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR634603-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR634603-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="343" mass="38507" checksum="0833FFEA1226A11D" modified="2009-02-10" version="1">MGKIKSVKFKLNVFEYEKPFHITNSISTKTSNIEVRIELENGILGFGEASPSFRVNGEKVEALLALEPVINEMIKGYDTKNYRQIFDITDKLFAFPSIKAAVQYAVLDALSEEIDLPVYKILGGAKGKIETDKTVSIGTLEERVNDATKIFEEGFRIIKIKVGENLREDIETIERIYEKTSGAKYIVDANMGYTPKEAITFVNELYKKGIDINVFEQPVKMHDIEGLKFVRFHSPFPVGADESAKTKYDVMRLIKEEAVDYVNIKLMKSGISDALAIVEIVKAANLHLMIGCMGESSLGINQSVHFALGTGAFDFHDLDSALIIKEENFRGKYKTEIPYHIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEF7</accession>
    <name>B7IEF7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1962</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TspO/BZRP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580510.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76384">
        <property type="protein sequence ID" value="ACJ76384"/>
        <property type="gene ID" value="THA_1962"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1962"/>
    <dbReference type="eggNOG" id="COG3476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091805_2_0_0"/>
    <dbReference type="OMA" id="RDNQGWR"/>
    <dbReference type="OrthoDB" id="1679387at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15904">
        <property type="entry name" value="TSPO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038330">
        <property type="entry name" value="TspO/MBR-related_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004307">
        <property type="entry name" value="TspO_MBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057">
        <property type="entry name" value="PTHR10057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03073">
        <property type="entry name" value="TspO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005859">
        <property type="entry name" value="PBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="163" mass="18993" checksum="8CC1D9C7DC313324" modified="2009-02-10" version="1">MVKKTTNCPHKISKFIASVVGVSLIGVIILFLIKKPFNTWYVYLNKPFFSPPDWMFTSVWTGIAILIGLSLFFAWEENFGRKKNLAIGIFIIQIILNISWFLVFFNLKNLLMAFFIDLILFIVLLFNTIVFYKINKTTGRLLIPYLLWVGYFTFVNLGLYVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEG1</accession>
    <name>B7IEG1_THEAB</name>
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        <submittedName>
            <fullName evidence="6">Inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1967</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580514.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1967"/>
    <dbReference type="EnsemblBacteria" id="ACJ76388">
        <property type="protein sequence ID" value="ACJ76388"/>
        <property type="gene ID" value="THA_1967"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1967"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_0"/>
    <dbReference type="OMA" id="YKIVYFF"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="70"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="292" mass="33471" checksum="E87A927A11BA5664" modified="2009-02-10" version="1">MASKLRKRENYLGWGFFSIYLIYTAIFWGYPFVWLVILSFSKWNFFGSPRPVGFKNFIRLFSDPIFWRIFLNTVNFMVYFIPMVLILSMLFALALYRVNIFRTFFALSFLVANVSSGVAYSILFSNLFSESGPINSFLYKNFGITVPWFSDPQLALLSIAIMVTWKFVGYYGLILFAGLNAIPKSLYEAAELDGATNWQKFWKITFPLINPALTTVLVFAVNLTFGIFTEPYMITGGGPMRRTLTFMMHIYTTAFQRMDPSYAASLAVVTGLLSYGCVLLVRFLVEREVSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEJ0</accession>
    <name>B7IEJ0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103074.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76417">
        <property type="protein sequence ID" value="ACJ76417"/>
        <property type="gene ID" value="THA_1996"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1996"/>
    <dbReference type="eggNOG" id="ENOG502ZV0U">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2604872_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="79" mass="9053" checksum="9F1ED4A18ED5EE3E" modified="2009-02-10" version="1">MINRLINFYLFVVLQLFISFVVITIGFGIITGSFGLGLLEINKENMEFLKLITPTILNILKTLSFISFAVFIITFFLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEM1</accession>
    <name>B7IEM1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldehyde oxidase and xanthine dehydrogenase, molybdopterin binding</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_27</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579302.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_27"/>
    <dbReference type="EnsemblBacteria" id="ACJ74535">
        <property type="protein sequence ID" value="ACJ74535"/>
        <property type="gene ID" value="THA_27"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_27"/>
    <dbReference type="eggNOG" id="COG1529">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_3_0"/>
    <dbReference type="OMA" id="THYYQTV"/>
    <dbReference type="OrthoDB" id="117507at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="PTHR11908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="Ald_Xan_dh_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="SSF54665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="SSF56003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ald_Xan_dh_C" evidence="1">
        <location>
            <begin position="18"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="760" mass="83841" checksum="5FE7DB985E64F956" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEN9</accession>
    <name>B7IEN9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_45</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103373.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_45"/>
    <dbReference type="EnsemblBacteria" id="ACJ74553">
        <property type="protein sequence ID" value="ACJ74553"/>
        <property type="gene ID" value="THA_45"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_45"/>
    <dbReference type="HOGENOM" id="CLU_1776568_0_0_0"/>
    <dbReference type="OMA" id="FDINFKQ"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="146" mass="17065" checksum="88128DB419C0EAB5" modified="2009-02-10" version="1">MSLKLKIANLILIIAFLFGILFALYDFLFIKISFLKKFNQKTIILDRKISDFKILLTQYNNAKLFLNKDSHFSSEIAKYLDENKRLSNTLEREEFEKFFYNLVNSKSIIINKLNVDVNVDFPLIFNSKSKANISLDLEVGDIIDIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEQ3</accession>
    <name>RL19_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplS</name>
        <name type="ordered locus">THA_59</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579323.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IEQ3"/>
    <dbReference type="SMR" id="B7IEQ3"/>
    <dbReference type="STRING" id="484019.THA_59"/>
    <dbReference type="EnsemblBacteria" id="ACJ74567">
        <property type="protein sequence ID" value="ACJ74567"/>
        <property type="gene ID" value="THA_59"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_59"/>
    <dbReference type="eggNOG" id="COG0335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103507_2_1_0"/>
    <dbReference type="OMA" id="TITVYYE"/>
    <dbReference type="OrthoDB" id="1698718at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00402">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="L19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_L19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018257">
        <property type="entry name" value="Ribosomal_L19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="PTHR15680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002191">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00061">
        <property type="entry name" value="RIBOSOMALL19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01024">
        <property type="entry name" value="rplS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01015">
        <property type="entry name" value="RIBOSOMAL_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000193910" description="50S ribosomal protein L19">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00402"/>
        </source>
    </evidence>
    <sequence length="115" mass="13396" checksum="DBA783E280FA83E1" modified="2009-02-10" version="1">MSMDNLVRIIEKDQIKDVPEFRPGDTVKVYVRFKEGNKERTQAFEGIVISMRGSGISKTFTVRRIGANGIGVERIFPLYAPIIEKIEVVRRGKVRRAKLYYLREVRGKVKIKERR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEQ5</accession>
    <name>TRMD_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine-N(1)-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmD</name>
        <name type="ordered locus">THA_61</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates guanosine-37 in various tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA methyltransferase TrmD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579324.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IEQ5"/>
    <dbReference type="SMR" id="B7IEQ5"/>
    <dbReference type="STRING" id="484019.THA_61"/>
    <dbReference type="PRIDE" id="B7IEQ5"/>
    <dbReference type="EnsemblBacteria" id="ACJ74569">
        <property type="protein sequence ID" value="ACJ74569"/>
        <property type="gene ID" value="THA_61"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_61"/>
    <dbReference type="eggNOG" id="COG0336">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047363_0_1_0"/>
    <dbReference type="OMA" id="ILCGHYK"/>
    <dbReference type="OrthoDB" id="525632at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N(1))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18080">
        <property type="entry name" value="TrmD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1270.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00605">
        <property type="entry name" value="TrmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002649">
        <property type="entry name" value="tRNA_m1G_MeTrfase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023148">
        <property type="entry name" value="tRNA_m1G_MeTrfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016009">
        <property type="entry name" value="tRNA_MeTrfase_TRMD/TRM10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46417">
        <property type="entry name" value="PTHR46417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01746">
        <property type="entry name" value="tRNA_m1G_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000386">
        <property type="entry name" value="tRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00088">
        <property type="entry name" value="trmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000130218" description="tRNA (guanine-N(1)-)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="131"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00605"/>
        </source>
    </evidence>
    <sequence length="246" mass="28482" checksum="44376289FB8D837E" modified="2009-02-10" version="1">MKISVLTIFPEMVEVIKKYGVISRAVQENKIEINIFNLRDFTSDKHRTVDDYPFGGGPGMVMKPEPFFRFYDFYVQAFGKPHTILTSPQGRTFNNDLVKELSLKENLLIICGRYEGIDERVMELVDDEISIGDYVLTGGELPAMVMIDAISRFVPGVISDESVIEESFNTGLLDHPHYTRPREFNGRKVPEVLLEGNHEKIELWRRKMSLKKTIQRRPDLFLKKEFDEVDKIALLELLRELINDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEQ7</accession>
    <name>B7IEQ7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-binding protein KhpA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">KH-domain protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">khpA</name>
        <name evidence="2" type="ordered locus">THA_63</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">A probable RNA chaperone. Forms a complex with KhpB which binds to cellular RNA and controls its expression. Plays a role in peptidoglycan (PG) homeostasis and cell length regulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex with KhpB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the KhpA RNA-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103397.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_63"/>
    <dbReference type="EnsemblBacteria" id="ACJ74571">
        <property type="protein sequence ID" value="ACJ74571"/>
        <property type="gene ID" value="THA_63"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_63"/>
    <dbReference type="eggNOG" id="COG1837">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132074_1_0_0"/>
    <dbReference type="OMA" id="AVKMDKR"/>
    <dbReference type="OrthoDB" id="1940160at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00088">
        <property type="entry name" value="UPF0109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020627">
        <property type="entry name" value="KhpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34654">
        <property type="entry name" value="PTHR34654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0133">Cell shape</keyword>
    <keyword evidence="1" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="74" mass="8364" checksum="8CCE85E78E026E3F" modified="2009-02-10" version="1">MKELLEYMLKGIVKNPDEVVVLEFEESGKKVFEISVNPEDVGQVIGKDGRTIKSIKILLSSITDENNFILKVVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEU8</accession>
    <name>HFQ_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-binding protein Hfq</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hfq</name>
        <name type="ordered locus">THA_104</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Hfq family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103489.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IEU8"/>
    <dbReference type="SMR" id="B7IEU8"/>
    <dbReference type="STRING" id="484019.THA_104"/>
    <dbReference type="EnsemblBacteria" id="ACJ74612">
        <property type="protein sequence ID" value="ACJ74612"/>
        <property type="gene ID" value="THA_104"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_104"/>
    <dbReference type="eggNOG" id="COG1923">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113688_0_2_0"/>
    <dbReference type="OMA" id="QQMVYKH"/>
    <dbReference type="OrthoDB" id="1989988at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01716">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00436">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005001">
        <property type="entry name" value="Hfq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34772">
        <property type="entry name" value="PTHR34772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17209">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02383">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_1000190366" description="RNA-binding protein Hfq">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00436"/>
        </source>
    </evidence>
    <sequence length="84" mass="9839" checksum="9203DA195D9B9EF5" modified="2009-02-10" version="1">MAEKFNLQDRFLNILRTNKIPVKVYLVNGFQTKGIIRSFDNFTMLLENGSQQNLIYKHAVSTIMPESFVKLTKQQNEESENEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEU9</accession>
    <name>B7IEU9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTPase HflX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GTP-binding protein HflX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 10" type="primary">hflX</name>
        <name evidence="10" type="ordered locus">THA_105</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">May associate with membranes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_105"/>
    <dbReference type="EnsemblBacteria" id="ACJ74613">
        <property type="protein sequence ID" value="ACJ74613"/>
        <property type="gene ID" value="THA_105"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_105"/>
    <dbReference type="eggNOG" id="COG2262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019597_2_2_0"/>
    <dbReference type="OMA" id="AEMKTGR"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01878">
        <property type="entry name" value="HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00900">
        <property type="entry name" value="GTPase_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030394">
        <property type="entry name" value="G_HFLX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032305">
        <property type="entry name" value="GTP-bd_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016496">
        <property type="entry name" value="GTPase_HflX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025121">
        <property type="entry name" value="GTPase_HflX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042108">
        <property type="entry name" value="GTPase_HflX_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229">
        <property type="entry name" value="PTHR10229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13167">
        <property type="entry name" value="GTP-bdg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006809">
        <property type="entry name" value="GTP-binding_hflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03156">
        <property type="entry name" value="GTP_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51705">
        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hflx-type G" evidence="9">
        <location>
            <begin position="169"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="175"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="200"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="222"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="288"/>
            <end position="291"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="312"/>
            <end position="314"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="336" mass="38954" checksum="79298ADB6A22C47B" modified="2009-02-10" version="1">MEELCETIGIKVVDKIFQKRNRPNPSFYIGKGLLEKVKEFFIRENIDYLVIDDNISHIQRRNIEKYLEKKVYDRTEIILEIFAKHAKTNEGRLQVEIARLKYLLPYLVGQGKELSRLGGGIGTRGPGEKSLEYSKRYIKNRIKKLESKLKKLESIRQVQRKKRNQSNIYKVSIVGYTNAGKTTLLSNLAKETLLSKNEMFTTLSPVSRRVLLPSGRYAIFSDTVGFIRNLHPLIIEAFHSTLEEINFSDLIIILVDVADKNFEEKLEIIDETLESINIAKIKTLLVFNKIDLCHKDYIKNLQRRFSNAVFISAKNKENISTLLHQVENLLQKEVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEW5</accession>
    <name>B7IEW5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphate ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pstB</name>
        <name evidence="5" type="ordered locus">THA_121</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103526.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_121"/>
    <dbReference type="EnsemblBacteria" id="ACJ74629">
        <property type="protein sequence ID" value="ACJ74629"/>
        <property type="gene ID" value="THA_121"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_121"/>
    <dbReference type="eggNOG" id="COG1117">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_0"/>
    <dbReference type="OMA" id="TIDICRV"/>
    <dbReference type="OrthoDB" id="1416748at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015415">
        <property type="term" value="F:ATPase-coupled phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="Phosp_transpt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="PTHR43423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00972">
        <property type="entry name" value="3a0107s01c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="250" mass="28137" checksum="0515B4C8BF57876E" modified="2009-02-10" version="1">MEIIEFKNFGAYYGEKKVVNNVTFPVYINKITAIIGPSGCGKSTLLRSINRINDEIPGFKVEGSLLIDGKDVYKDVDDLTVLRKKVGMVFQRPVPFPMSIYENIAFGLKIHGVKDKSKINKIVEKTLKEAALWDEVKHELDKNAYSLSGGQQQRLCIARSLAIEPEVLLLDEPTSALDPIATQRIESLLERLSEKYTIVIVTHNIAQAIRISDYIAFMFKGELIEFGETSEVVRNPKNKLTEEYLNGKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEX2</accession>
    <name>B7IEX2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative dihydroflavonol 4-reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579364.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_128"/>
    <dbReference type="EnsemblBacteria" id="ACJ74636">
        <property type="protein sequence ID" value="ACJ74636"/>
        <property type="gene ID" value="THA_128"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_128"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_0_0"/>
    <dbReference type="OMA" id="SVRMMRA"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="338" mass="38205" checksum="C7F28E7BB158C39B" modified="2009-02-10" version="1">MIFITGGSGHLGNVLIRKLLKMGEQVVTLVHPSDKCESLKGLNVKIVKGDVRNYTLVEKLSKNADIIIHLAALISILPWKKKAVYSVNIDGTKNILKVCKKLNKKLIYISSVHAFEEPKPGTVIDENTNIDPSKTSGVYGKSKAFAALEVLNAIKSGLDITTVCPTGIIGPFDFKPSEMGIMFLKYLTNKLKYIIDGSFDFVDVRDVADGIIKLIYLDKRANFYILSNKTFKMTELIHLLNEITGKNIKPKVINTNFAYFLSLFSTTFGYLVNKKPLFTPYSIHTLTRNYTFSHEKATKEINYNPRDIKITLYDTLEWLSNYFLKEKLINPLNLHSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF33</accession>
    <name>B7IF33_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Oxaloacetate decarboxylase beta chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_192</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579401.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_192"/>
    <dbReference type="EnsemblBacteria" id="ACJ74697">
        <property type="protein sequence ID" value="ACJ74697"/>
        <property type="gene ID" value="THA_192"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_192"/>
    <dbReference type="eggNOG" id="COG1883">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036168_0_0_0"/>
    <dbReference type="OMA" id="PMIADPK"/>
    <dbReference type="OrthoDB" id="662061at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005661">
        <property type="entry name" value="OadB_MmdB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35806">
        <property type="entry name" value="PTHR35806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03977">
        <property type="entry name" value="OAD_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015658">
        <property type="entry name" value="MmdB_OadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01109">
        <property type="entry name" value="Na_pump_decarbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="7" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="360"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR015658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="378" mass="40314" checksum="B227406C77AC63EA" modified="2009-02-10" version="1">MLESLMAFFQSTAFMNLTIGNIVMFAVAGVLIYIAIAKDAEPLLLIPIAFGIILANVPPDVTGILNPPTETSPGGVLWYLQQGLALGIYPPLIFLGIGALTDFSFMLSYPITIFLGGAAQMGIFITFLLAKFFGFSLKQAASIGIIGGADGPTSIYITSKFAPELLSIIAISAYSYIALIPVLQPMVSKMLTTKEERRIRMKPPRKVTKTERVLFPIVTTIIVALIIPKSLPLIGMLMFGNLLREAGVVKRLVEAASRYILDTVTILLCVSVGASARADIFLTPKSLLVFGMGAVAFVTAITFGILFAKLMNLFLKDKINPLIGAAGVSAVPDSARVAQRIAQEEDPTNFILMHAMSPNVAGVIGSAVAAGIFLSLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF70</accession>
    <name>B7IF70_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sugar ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_229</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103771.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_229"/>
    <dbReference type="EnsemblBacteria" id="ACJ74734">
        <property type="protein sequence ID" value="ACJ74734"/>
        <property type="gene ID" value="THA_229"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_229"/>
    <dbReference type="eggNOG" id="COG4603">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040769_0_2_0"/>
    <dbReference type="OMA" id="FRMNLFN"/>
    <dbReference type="OrthoDB" id="974216at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="344" mass="35701" checksum="F4D17457C57D1E64" modified="2009-02-10" version="1">MNKRFSAILIPIISVLIALAISTVIILLIGKNPLTAYSALIRGSFGSKQAIADTIIKTTPLILTGLAVGFGFRAGVFNIGAEGQMVMGALIAAAFAGNFGFLPPIIAIPLTILIAMAVGAGYAAIAGFLKAKTGAHEVVTTIMLNWIATYFASYMVTGPLKVGSGTPKSPEIANSAQLPILMKVGAIEVSAGIIIAVIAAIFMYILLNKSTTGYEIKAVGFNPYAAEYGGISVSKNIILAMAISGALAGLAGATELMGVHHRFLGELSGGKGFDGISIALIGQNNPIGIIFAALLIGALRTGSNEMQFVGVPKHMVMIIQGIVIFLVAADRIVRTIMIRKKVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF77</accession>
    <name>B7IF77_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ClpC ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_238</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579434.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_238"/>
    <dbReference type="EnsemblBacteria" id="ACJ74741">
        <property type="protein sequence ID" value="ACJ74741"/>
        <property type="gene ID" value="THA_238"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_238"/>
    <dbReference type="eggNOG" id="COG0542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005070_4_1_0"/>
    <dbReference type="OrthoDB" id="44062at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clp R" evidence="9">
        <location>
            <begin position="2"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="8">
        <location>
            <begin position="418"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="426"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="819" mass="93038" checksum="05FFABD643224F63" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFC5</accession>
    <name>B7IFC5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_289</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579473.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_289"/>
    <dbReference type="EnsemblBacteria" id="ACJ74789">
        <property type="protein sequence ID" value="ACJ74789"/>
        <property type="gene ID" value="THA_289"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_289"/>
    <dbReference type="eggNOG" id="COG1284">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063199_0_0_0"/>
    <dbReference type="OMA" id="AQIIHKY"/>
    <dbReference type="OrthoDB" id="1747063at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019264">
        <property type="entry name" value="DUF2179"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003740">
        <property type="entry name" value="YitT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10035">
        <property type="entry name" value="DUF2179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02588">
        <property type="entry name" value="YitT_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006483">
        <property type="entry name" value="Membrane_protein_YitT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2179" evidence="8">
        <location>
            <begin position="226"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="287" mass="31785" checksum="E67E477041C8021F" modified="2009-02-10" version="1">MAKNIPLRETIKEYIFSTIGVILTALGLVIFLIPNNIAAGGASGLAIILHSIIPLSVGVWMYILNAILFLIAFLIIGFDFSYKTIYCTFLLNFFIDLFDRIINIPTYNGGDLFLAVFFGDILTAIGMAITFSQNASTGGTDILAKIFNKFFSTPMGTTLLIIDFTIGFFAGVAFNPKIGMYSILAIIINGITIDFVLKGLELAITVTIISDNNKPIEEFILTNMGRGFTYLKGKGGYTKKDRDIIFVSIRRRELGELINFVKKVDPNAFMIVNESRYVLGEGFKRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFH3</accession>
    <name>B7IFH3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_337</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103990.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_337"/>
    <dbReference type="EnsemblBacteria" id="ACJ74837">
        <property type="protein sequence ID" value="ACJ74837"/>
        <property type="gene ID" value="THA_337"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_337"/>
    <dbReference type="eggNOG" id="COG1146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_3_0"/>
    <dbReference type="OMA" id="CARMCPD"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="42"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="73" mass="8328" checksum="A7373F508884BAE1" modified="2009-02-10" version="1">MPKVKGYIEIDQERCKGCGLCINACPMKVIEFSDSFNSKGYHPAEPKHLEKCIACGFCYRMCPDVCITVYREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFJ8</accession>
    <name>B7IFJ8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SHOCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_368"/>
    <dbReference type="EnsemblBacteria" id="ACJ74862">
        <property type="protein sequence ID" value="ACJ74862"/>
        <property type="gene ID" value="THA_368"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_368"/>
    <dbReference type="eggNOG" id="COG3462">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159099_2_3_0"/>
    <dbReference type="OMA" id="MWRGFCH"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018649">
        <property type="entry name" value="SHOCT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09851">
        <property type="entry name" value="SHOCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHOCT" evidence="1">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="61" mass="7239" checksum="20257CC13BBA3EDB" modified="2009-02-10" version="1">MFFGLILLAVILYFLFKTFKPSFKGEFEDSALKILNEKLAKGEITEEEYKRKKELIMKGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFL4</accession>
    <name>B7IFL4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 type transporter family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_385</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579539.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_385"/>
    <dbReference type="EnsemblBacteria" id="ACJ74878">
        <property type="protein sequence ID" value="ACJ74878"/>
        <property type="gene ID" value="THA_385"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_385"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_0_0_0"/>
    <dbReference type="OMA" id="KWIVVIN"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="6">
        <location>
            <begin position="148"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="351" mass="40324" checksum="E89BF4D66A6519A8" modified="2009-02-10" version="1">MFQALFKIEFLRSKEATFWIIVFPILLTILLTSIFGDIEKNVKFRIGYIGENKFIENLKDHFEVLKVKKVEEIVEKNLDAVIVFEDNFDLKLQRALILSKTKLFKPVNVEIFYTDKEYSKVVKDILVNIFSSLNALNSEKEVKLKVNERKKSISYSQFLYASILVMNIMSVTFFGFTSQISFYKRRGLLRRLKCTPYSLGKFYFVYAIVSLMQIIFSSVIFTLFDFLVYGVKPDVLNVSIYMLFGSVVFLSLGYMIASLIENPETIVVLGNILFQVFMFAGGLYFDVRNAGIIGTLSKIIPSTYLVDGIRKSFGYSAYENHFLIPLIWIIVSLSIVALSSIIKRKKASSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFL8</accession>
    <name>B7IFL8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase, AAA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_389</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. RarA/MGS1/WRNIP1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104115.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_389"/>
    <dbReference type="EnsemblBacteria" id="ACJ74882">
        <property type="protein sequence ID" value="ACJ74882"/>
        <property type="gene ID" value="THA_389"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_389"/>
    <dbReference type="eggNOG" id="COG2256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017985_0_3_0"/>
    <dbReference type="OMA" id="RIILSQC"/>
    <dbReference type="OrthoDB" id="977094at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032423">
        <property type="entry name" value="AAA_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021886">
        <property type="entry name" value="MgsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16193">
        <property type="entry name" value="AAA_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12002">
        <property type="entry name" value="MgsA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="37"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="410" mass="45980" checksum="B05AFB320094BB93" modified="2009-02-10" version="1">MSGLSEILRPKNFEDVVGQEHILGEKGILKLAIEKDSLFSAIFYGPPGCGKTSTLEVIKLNTSYEVYHFNAAITSTAEVKKILEYAAKVKDVKKILIFIDEFHRFNKKQQDIFLPGVEAFDYVLIGATTENPYKMVNPALLSRVRLVAFKKLQKEHIIKLLEKAVNVKKVDINENAKDFIARVSNGDGRFAINLYEILSDIAISIDKKIIDEEILQIYSSDENRVYTAKEHYNLASAFIKSIRGSDPDAALYYMARMIEGGEDPRFIARRLVILASEDIGLADPMALLVATSTAQAVELVGLPECMLNLSQCVIYLSLAPKSNSSTLAIQKALEVAKDTMNLDVPRDLLNIPDSGYKNPHKFGGFLKKSYLPKEISDKVFYTPKSIGKEKKNIEILETLWRGVKQYDDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFV0</accession>
    <name>B7IFV0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase</fullName>
            <ecNumber evidence="4">4.6.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_473</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP</text>
            <dbReference type="Rhea" id="RHEA:23864"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:58483"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="4.6.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.12" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579602.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_473"/>
    <dbReference type="EnsemblBacteria" id="ACJ74964">
        <property type="protein sequence ID" value="ACJ74964"/>
        <property type="gene ID" value="THA_473"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_473"/>
    <dbReference type="eggNOG" id="COG0245">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_839206_0_0_0"/>
    <dbReference type="OMA" id="MIYIIER"/>
    <dbReference type="OrthoDB" id="525632at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00095"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008685">
        <property type="term" value="F:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00554">
        <property type="entry name" value="MECDP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003526">
        <property type="entry name" value="MECDP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020555">
        <property type="entry name" value="MECDP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036571">
        <property type="entry name" value="MECDP_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43181">
        <property type="entry name" value="PTHR43181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02542">
        <property type="entry name" value="YgbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69765">
        <property type="entry name" value="SSF69765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01350">
        <property type="entry name" value="ISPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YgbB" evidence="8">
        <location>
            <begin position="183"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="332" mass="37946" checksum="1EBE984B32447CB0" modified="2009-02-10" version="1">MKFTTINEDNYIDCAKFIYEEKKEYFDYLFKKDAFEIIKKAILFNIPPFLMENSLVIKSDEGKILGVLLYASKSAFRHRYEKWIKILGFKVLGIGVKLASIIGQILLNFSVDDVYIISLSAQDFYLEYELLYRFVISHDYKRILTDVTEGLIEKYKRMGFVVEKGLDNKIFRMYKKSKLNIASGIGWDTHPIKKDRKLILGGVEIESDIGLDGHSDADVLIHSIVDSFVGVSLKKDIGELFPEKDVPKGISSVFILENVLKSMKENGFFPESVDCVLITDFRLGKYRAEISKNLEKIINCPVSIKFKTGNNVYPETNLKAMTAMCVSNIISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFW1</accession>
    <name>B7IFW1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DAK2 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_484</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579611.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_484"/>
    <dbReference type="EnsemblBacteria" id="ACJ74975">
        <property type="protein sequence ID" value="ACJ74975"/>
        <property type="gene ID" value="THA_484"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_484"/>
    <dbReference type="eggNOG" id="COG1461">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017496_1_0_0"/>
    <dbReference type="OMA" id="TRTIPQG"/>
    <dbReference type="OrthoDB" id="1554133at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004371">
        <property type="term" value="F:glycerone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019986">
        <property type="entry name" value="DAK2_dom-cont_prot_YloV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004007">
        <property type="entry name" value="DhaL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036117">
        <property type="entry name" value="DhaL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033470">
        <property type="entry name" value="UPF_DhaK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13684">
        <property type="entry name" value="Dak1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02734">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01121">
        <property type="entry name" value="Dak1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01120">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101473">
        <property type="entry name" value="SSF101473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03599">
        <property type="entry name" value="YloV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51480">
        <property type="entry name" value="DHAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DhaL" evidence="1">
        <location>
            <begin position="7"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="537" mass="60460" checksum="66518696940CD354" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFY0</accession>
    <name>B7IFY0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_505"/>
    <dbReference type="EnsemblBacteria" id="ACJ74994">
        <property type="protein sequence ID" value="ACJ74994"/>
        <property type="gene ID" value="THA_505"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_505"/>
    <dbReference type="HOGENOM" id="CLU_2048605_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="120" mass="14021" checksum="4243D9268BC0CA12" modified="2009-02-10" version="1">MRLKDELILLLRKGFYVLLMVFILMFVAISILLFNFEKNNLVNFASFVKSAVEEKSFESSESVINYINKLIANKEEFMFFSPAKENRLFYVSVPIKVLGYNFVICKDLRSSFLKLVIILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFZ3</accession>
    <name>B7IFZ3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyruvate oxidoreductase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_518</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100657.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_518"/>
    <dbReference type="EnsemblBacteria" id="ACJ75007">
        <property type="protein sequence ID" value="ACJ75007"/>
        <property type="gene ID" value="THA_518"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_518"/>
    <dbReference type="eggNOG" id="COG1013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058423_0_0_0"/>
    <dbReference type="OMA" id="ATGCMEV"/>
    <dbReference type="OrthoDB" id="981516at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="2">
        <location>
            <begin position="55"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="325" mass="36170" checksum="242E286FB08415EB" modified="2009-02-10" version="1">MALNIMQLATMFADKHPGITSGHRLCPGCAAPNVAKFALMTAEALGYTPVVGFATGCMEVSTTIYPYTAWNVPYIHNAFENVAATVSGVETAYRALLKKGRLVDPNKKYAFFAFGGDGGTYDIGLQALSGAVERGHKFIYIVYDNEGYMNTGNQRSGATPPGSQTTTQPVGKKLSGKIQLKKNIVEIMAAHENVYVATVSTSEPMDFFKKIEKALNFDGPSFIAAMSPCVRFWRVNDDKAVEISKLAVETGYWPLYEVERGVYRVTKKVKELKPVREFIEKQGRFRPLLKRPDADEIINELQEYVTSRWERLLALEEATKDKPIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG13</accession>
    <name>B7IG13_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="8">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">trpS</name>
        <name evidence="10" type="ordered locus">THA_539</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tryptophan to tRNA(Trp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-tryptophanyl-tRNA(Trp)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100615.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_539"/>
    <dbReference type="EnsemblBacteria" id="ACJ75027">
        <property type="protein sequence ID" value="ACJ75027"/>
        <property type="gene ID" value="THA_539"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_539"/>
    <dbReference type="eggNOG" id="COG0180">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029244_0_0_0"/>
    <dbReference type="OMA" id="GWGQFKP"/>
    <dbReference type="OrthoDB" id="951354at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_B">
        <property type="entry name" value="Trp_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024109">
        <property type="entry name" value="Trp-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="9"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="193"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="8"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="16"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="193"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="331" mass="38081" checksum="1223F2485E621326" modified="2009-02-10" version="1">MRVLSGMRPTGKLHIGHLFGALSSWVELQEKGHECFYFVADWHALTTHYNDTKEIVNNSIELVKSYLAVGIDPNKSTIFIQSEIKEHAELTLLFSMFVSVSRLERVPTYKEIKQQLSDKDLSNAGFLIYPVLQAADILIYRATGVPVGEDQVYHIELTREIARRFNYLYKEVFPEPEPILSKVPKLLGTDGRKMSKSYGNTIPLVADEETLKKMVMPMITDPARQRRTDPGNPEKCPVWDYHKAFGISDKDAEWVKDGCTNAKIGCVDCKKLLLKNMKERLEPIWERYNSLTDDYVKDVIVEGNKKARQVARETIEMVRDAMQLDYNFLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG57</accession>
    <name>B7IG57_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lipophilic protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_584</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579659.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_584"/>
    <dbReference type="EnsemblBacteria" id="ACJ75071">
        <property type="protein sequence ID" value="ACJ75071"/>
        <property type="gene ID" value="THA_584"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_584"/>
    <dbReference type="eggNOG" id="COG0472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023982_2_0_0"/>
    <dbReference type="OMA" id="WRLLGHF"/>
    <dbReference type="OrthoDB" id="728505at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="310" mass="35108" checksum="5584F97EC6D1FC81" modified="2009-02-10" version="1">MLYAFVGSFILTLILIPFLRKIAFKFGIVDKPDNKLKNHEKSTPYLGGAAFVFSFLIFTPFSLFRKMYIVVLSLMGLYDDLKSINPWLRLTIEFIIGFLVSTRFLTNPIEITIATIFYAFLINSVNMMDGMDGVCAITSTIAAFGLIWTVTFPNDKYYLISLIGSLLAYLIFNFPPAKIFMGDMGSYTIGAILGVSVISSFSKSLSHTIAALIILSPFFLDTFTSMTRRVLAGKSPFEGDRDHIYDKLQKRLKDKRKTFFTMALISSIFCLLGILYIKYDKLSLFLTGFLMTILTIKLSLLKYDNKETEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG93</accession>
    <name>B7IG93_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acylamino-acid-releasing enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_621</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579689.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_621"/>
    <dbReference type="MEROPS" id="S09.071"/>
    <dbReference type="EnsemblBacteria" id="ACJ75107">
        <property type="protein sequence ID" value="ACJ75107"/>
        <property type="gene ID" value="THA_621"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_621"/>
    <dbReference type="eggNOG" id="COG1506">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008615_2_2_0"/>
    <dbReference type="OMA" id="DENHWIM"/>
    <dbReference type="OrthoDB" id="821953at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S9" evidence="2">
        <location>
            <begin position="436"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="646" mass="75221" checksum="DEA48F84A0E853A1" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGA3</accession>
    <name>B7IGA3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">30S ribosomal protein S18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">rpsR</name>
        <name evidence="5" type="ordered locus">THA_637</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 30S ribosomal subunit. Forms a tight heterodimer with protein S6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579695.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_637"/>
    <dbReference type="EnsemblBacteria" id="ACJ75117">
        <property type="protein sequence ID" value="ACJ75117"/>
        <property type="gene ID" value="THA_637"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_637"/>
    <dbReference type="eggNOG" id="COG0238">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148710_2_2_0"/>
    <dbReference type="OMA" id="QKKYCRF"/>
    <dbReference type="OrthoDB" id="1940575at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00270">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_S18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_S18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="PTHR13479"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00974">
        <property type="entry name" value="RIBOSOMALS18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="SSF46911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00165">
        <property type="entry name" value="S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="74" mass="9014" checksum="456025C51768E3CA" modified="2009-02-10" version="1">MKYRKKRKPKVCKLCQSKVEYVDYKDIKLLREFLTEKEKIIPRRITGNCAKHQRMVKIAIKRARQMALLPYVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGD7</accession>
    <name>B7IGD7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Anaerobic ribonucleoside-triphosphate reductase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_676</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579720.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_676"/>
    <dbReference type="EnsemblBacteria" id="ACJ75151">
        <property type="protein sequence ID" value="ACJ75151"/>
        <property type="gene ID" value="THA_676"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_676"/>
    <dbReference type="eggNOG" id="COG0602">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089926_0_1_0"/>
    <dbReference type="OMA" id="EPLAPYN"/>
    <dbReference type="OrthoDB" id="1141206at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043365">
        <property type="term" value="F:[formate-C-acetyltransferase]-activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012837">
        <property type="entry name" value="NrdG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PTHR30352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352:SF2">
        <property type="entry name" value="PTHR30352:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="172" mass="20388" checksum="0D2C1912130CB9A1" modified="2009-02-10" version="1">MTVYVNRIFFSTNDHPKLLGISIYFQGCDRSPKCLFCHNKETWEPYKGFKYDLEDLIQILKDKINYAFESYDKIAVVYLGGEPLAPYNRDAVFQISKELKEEFSEKIVNTLYSWRTLEEIERQNLENYIEYMDELVLGPYDHTQRNVDEKGNVLFPASKNQKYIKLNKVLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGD9</accession>
    <name>B7IGD9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_681</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Ycf16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579722.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_681"/>
    <dbReference type="EnsemblBacteria" id="ACJ75153">
        <property type="protein sequence ID" value="ACJ75153"/>
        <property type="gene ID" value="THA_681"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_681"/>
    <dbReference type="eggNOG" id="COG0396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_0"/>
    <dbReference type="OMA" id="IMFDEPD"/>
    <dbReference type="OrthoDB" id="1214676at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="PTHR43204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="247" mass="27422" checksum="F6BA711391FD2E12" modified="2009-02-10" version="1">MLELRNVSHEVSGKKILNNISMKFISGRKYAILGTNGAGKSTIGYVMMGLDGYKPTSGQIFLNGNDITNFNVFERAKAGITLMWQEPARYDGLTIYTYLTLGGKLDVTKDEVEEALEKVGLSPKIYLNRFVDKSLSGGERKRVELASIILLKPKFVILDEPDSGIDLMSLDMINQVIDFIADYGGTPIVITHREEMAYNTEEAYLLCAGKILANGETKQVIQIFKNICDTCDHPNSPVSKELKIDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGE2</accession>
    <name>B7IGE2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Caax amino protease family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579725.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_684"/>
    <dbReference type="EnsemblBacteria" id="ACJ75156">
        <property type="protein sequence ID" value="ACJ75156"/>
        <property type="gene ID" value="THA_684"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_684"/>
    <dbReference type="eggNOG" id="COG1266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104565_0_0_0"/>
    <dbReference type="OMA" id="YPIIIHN"/>
    <dbReference type="OrthoDB" id="1996747at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="210" mass="24428" checksum="6E777000D7D27279" modified="2009-02-10" version="1">MHITISIFILLFYILLSKIFVKVVRISDPFKLHSLFAILTILYSILSARVLNLKLVDMGISFGDTKKGLLILTFIGIPLFIIAYITSKNAKIYNFRYLYFKSKWQIIYFWILVGPTEEFLFRGLIQSYLKLKYSSITAILLSSLLFALFHLLNVLTKNESWKLFFNLLPTRFIISIILGFSLVYSKSLIYPIIIHNIIDGISFSNIKKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGF3</accession>
    <name>B7IGF3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="15">NADH:ubiquinone oxidoreductase, na(+)-translocating, d subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">nqrD</name>
        <name evidence="15" type="ordered locus">THA_696</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579734.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_696"/>
    <dbReference type="EnsemblBacteria" id="ACJ75167">
        <property type="protein sequence ID" value="ACJ75167"/>
        <property type="gene ID" value="THA_696"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_696"/>
    <dbReference type="eggNOG" id="COG1347">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046659_1_1_0"/>
    <dbReference type="OMA" id="CIIMGRF"/>
    <dbReference type="OrthoDB" id="1782047at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016655">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011292">
        <property type="entry name" value="NqrD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003667">
        <property type="entry name" value="NqrDE/RnfAE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02508">
        <property type="entry name" value="Rnf-Nqr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006102">
        <property type="entry name" value="NQR_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01939">
        <property type="entry name" value="nqrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="11 15" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ACJ75167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="199" mass="22290" checksum="490F349E1C0DE8B4" modified="2009-02-10" version="1">MEKFKEVFLKNTWYENPVFVLVLGICSTLAVTNNLKNTFIMTIGVMLVTGFSNLTVSLLKNLIPSKVRMITQVLIISFYVIIVDIILKAYLPDVSKALGPYVGLIITNCIIMGRAEAFAQSNPPLISFWDGITSGFGYMIVLVAIAFFRELLGFGTIFGFKVMPENFVTWTIMIMPPSAFFMLALFIWIVKGFKKEGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGL3</accession>
    <name>B7IGL3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable 2-phosphosulfolactate phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_762</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2R)-O-phospho-3-sulfolactate + H2O = (2R)-3-sulfolactate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23416"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15597"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58738"/>
            <dbReference type="EC" id="3.1.3.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ComB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.71" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579785.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_762"/>
    <dbReference type="EnsemblBacteria" id="ACJ75227">
        <property type="protein sequence ID" value="ACJ75227"/>
        <property type="gene ID" value="THA_762"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_762"/>
    <dbReference type="eggNOG" id="COG2045">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070028_0_0_0"/>
    <dbReference type="OMA" id="RLFMSTT"/>
    <dbReference type="OrthoDB" id="1617556at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050532">
        <property type="term" value="F:2-phosphosulfolactate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005238">
        <property type="entry name" value="ComB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036702">
        <property type="entry name" value="ComB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37311">
        <property type="entry name" value="PTHR37311"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04029">
        <property type="entry name" value="2-ph_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142823">
        <property type="entry name" value="SSF142823"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="226" mass="25344" checksum="4652415C69A80AEB" modified="2009-02-10" version="1">MLDVRLVPTKIKDYQAIVVIDVLRATTTMITAISNGAQFLVPVETVEQARIYRNDGFLLCGERGGVKPEDFDLGNSPLEYTKDVVKDKKLVITTTNGTKALAKAREHSKNIILGAFVNFSKTLEYIKKFDDILFVCSGNDGEESFEDTQVAGAFIEKLVLLKEYELSDSALISLNFWKSIKRPNFTGKHSRVLKAYGFKDDIIFSQQLDIFNTVVIYDGDKVIKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGN2</accession>
    <name>B7IGN2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage II sporulation E family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100352.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_784"/>
    <dbReference type="EnsemblBacteria" id="ACJ75246">
        <property type="protein sequence ID" value="ACJ75246"/>
        <property type="gene ID" value="THA_784"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_784"/>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_703441_0_0_0"/>
    <dbReference type="OMA" id="KIVCGGT"/>
    <dbReference type="OrthoDB" id="533349at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="379" mass="42735" checksum="4B02C8B9792C60B8" modified="2009-02-10" version="1">MITCEINYASKNKKGEWVCGDSIKIKRNEEKCVVSVSDGLGSGVKANILSTLTATMATTMVFNDVPIKEVFTSIISTLPTCKVRKISYSTLATCLIDYKKKRCTIFEYEFPLIFYFRDGELLNLKKIVKKVENKKLTVSEIDIKIGDSIFLMTDGISQAGMGTDMYPFGFGEENIIFELKNLLKNKVEHENIVKHMIRLAEKLDRYSKGDDALIAGLKIREKRVLTIMVGPPEDESKDRIVVEKLLNSRGKKVICGGTTGQIVERITGKNIDVDVRTISKNSPPIGYMEGVDLVTEGIITLTQVFRYYENQIEELGIGAKKLIEMLEDADVINFLVGRAINPAHQNPLFTHDISLKFRLINDISKILEKRGKIINLEYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGQ5</accession>
    <name>B7IGQ5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Glutamine--fructose-6-phosphate aminotransferase [isomerizing]</fullName>
            <ecNumber evidence="2 8">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">GFAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glucosamine-6-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">L-glutamine--D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">glmS</name>
        <name evidence="11" type="ordered locus">THA_807</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="2 8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579809.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_807"/>
    <dbReference type="EnsemblBacteria" id="ACJ75269">
        <property type="protein sequence ID" value="ACJ75269"/>
        <property type="gene ID" value="THA_807"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_807"/>
    <dbReference type="eggNOG" id="COG0449">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_5_2_0"/>
    <dbReference type="OMA" id="ASEYRYA"/>
    <dbReference type="OrthoDB" id="43416at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00164">
        <property type="entry name" value="GlmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GlmS_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="PTHR10937:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="9">
        <location>
            <begin position="2"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="275"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="450"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GATase activity" evidence="8">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="For Fru-6P isomerization activity" evidence="8">
        <location>
            <position position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="601" mass="67247" checksum="38C90DA4BE2C98A4" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGQ6</accession>
    <name>B7IGQ6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase M16 inactive domain family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_808</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579810.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_808"/>
    <dbReference type="EnsemblBacteria" id="ACJ75270">
        <property type="protein sequence ID" value="ACJ75270"/>
        <property type="gene ID" value="THA_808"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_808"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_6_1_0"/>
    <dbReference type="OrthoDB" id="1077305at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M16" evidence="1">
        <location>
            <begin position="63"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="2">
        <location>
            <begin position="215"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="491" mass="56665" checksum="6E75647AF271A040" modified="2009-02-10" version="1">MKKIFKNLIYILIILAFSANLFAVEFSKELFDKLAGAKNKIPTIEIPEYERVQLDNGMVFYISKDTTLPIVEIKGYVKFGKMNETIETAGYSSVMLDIMNTATKKFSETELIDFKELNGLEISFSANNDYYTISANSLSEDLEALFEALASELIEPQFEGNHFDRIVKEYLQSIGQSYTTEDGLLNMYYSINVFGKDHPYSFSDNLELLYANISSLTPEKLRDFYYKTISPNRIIISIAGNFEIDNVKELIKKYFANWKNTASSEPLFVYDSSLKTTPKIVVVDRQDSTQAKIKMGYRFPNFEFFKDKLYDRVAFLMANRIYGSGAFKSRLMKVLRTEKGYVYGINSSFSTGSYLGNFYVTTTVRYDAIADLIKDVKKIMEDIKYYKEPLTSEELFNEVNLYNALFPNAYKDKITVLDSVAFDVEIRKINENYINEFIKMYNSLSAEDVQKAFSRFTYPNNFVTIIVGNKEKIIENLENNGIKDYEVIENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGR3</accession>
    <name>B7IGR3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MutT/nudix family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100412.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_815"/>
    <dbReference type="EnsemblBacteria" id="ACJ75277">
        <property type="protein sequence ID" value="ACJ75277"/>
        <property type="gene ID" value="THA_815"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_815"/>
    <dbReference type="eggNOG" id="COG0494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062658_5_1_0"/>
    <dbReference type="OMA" id="PGYFASK"/>
    <dbReference type="OrthoDB" id="1831818at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="38"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="181" mass="20730" checksum="14AD223B4D4E7085" modified="2009-02-10" version="1">MKEKKVKSEDIFKGVLLHLKKDEVLLQNGRYSTREYVLHPGAVAIVPVNENNEVVFVRQFRYPIGQDLLEIPAGKFDSPDEDPLECAKRELKEETGFSADKFIYMGYIFTTPGFSNEIIHLYLALDLQKGEMKPDDDEIIELEFKNLDQALKECIEGKITDAKTIAGIFKAYFYLKSIEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGU4</accession>
    <name>B7IGU4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptide amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_847</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579828.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_847"/>
    <dbReference type="EnsemblBacteria" id="ACJ75308">
        <property type="protein sequence ID" value="ACJ75308"/>
        <property type="gene ID" value="THA_847"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_847"/>
    <dbReference type="eggNOG" id="COG0154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_14_5_0"/>
    <dbReference type="OMA" id="DMRSNNY"/>
    <dbReference type="OrthoDB" id="1239251at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="460" mass="51636" checksum="302CD082D26AB600" modified="2009-02-10" version="1">MKNLTVKKFNDFYDEGKLTSERLVEFYLERISKINLNAILEINPDALFIARALDRERRNGKKRSNLHGIPVIIKGNIDTNDKMQTTAGAKALEGNFASSDAFIVKKLREAGCVIIGKANLTEFANFVSFKMPNGYSRLGGQTRNPYGDFDTGGSSSGSAVAIAADLALLSIGTETSGSILSPSSMNSCVGLKPTVGTVSRTGIIPISFTQDTAGPITRTVEDAFELFKVIFGYDHKDPATYFIKNFSFDSKIEIIHDLYGMKFGYTDQIFEWMNKELVDIFLENLKKIEKLGGKVKKVEFKNLNKINNIEVLYYEFKLGINNYLKDKNLKVKTLSDIIKYNFENRDAIPYGQNILLYSDATDIKDGRYIEYLLNDRKYAKGEIDRLFNENDLDALLFPANYGAHITAKAQYPSIVVPAGFTEKGPFGLTFSARAFEENKLFSIALLFEKSFSERKLPELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGV0</accession>
    <name>B7IGV0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_854</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579834.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_854"/>
    <dbReference type="EnsemblBacteria" id="ACJ75314">
        <property type="protein sequence ID" value="ACJ75314"/>
        <property type="gene ID" value="THA_854"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_854"/>
    <dbReference type="eggNOG" id="COG1337">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081549_0_0_0"/>
    <dbReference type="OMA" id="MWGALTN"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="275" mass="31983" checksum="3E7677635BEF8418" modified="2009-02-10" version="1">MESSSKGIWYKLVFKQVQPIHIGYKRYGVINETRIFIPGWTMWGALTNIYGLYTGSYEDIDEVFEEISCFWPSFDKGGNDVLFPSLRYGKLHLGNFSEEKFRTMFVDTIISTAISPVLRSAKDESLHEIDVILPRPREEFVRELDANISLYWVGILNIKDQKLLSFLKSGLEIFVGGDIKYGLGLLKLEYINECEEISKFKKNNFLEINEEEFNSENLDKMGELIVDIENTIDGALLKIKEEKLLFLWDTNNKEKIIDLENYKLEKGKLLKNFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGV2</accession>
    <name>B7IGV2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Crispr-associated ramp protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_856</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579836.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_856"/>
    <dbReference type="EnsemblBacteria" id="ACJ75316">
        <property type="protein sequence ID" value="ACJ75316"/>
        <property type="gene ID" value="THA_856"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_856"/>
    <dbReference type="eggNOG" id="COG1336">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047795_1_1_0"/>
    <dbReference type="OMA" id="IGRVDNT"/>
    <dbReference type="OrthoDB" id="972874at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013410">
        <property type="entry name" value="CRISPR-assoc_RAMP_Cmr4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36700">
        <property type="entry name" value="PTHR36700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="366" mass="41041" checksum="9C64ADEA9BC8730C" modified="2009-02-10" version="1">MVDHMLVTNKRIFAQAMDPIYIGTGGYNIGRVDNTIVRDPITNIPKIPGSSLAGTWRYYSALELVSRVKDYQPSFKDIKSINGENFSSKLKNAKWKNDFPFGEHDWESYPGNKVARIKCAGQDDLPNKSIDDIEEETGHCGHCIVCKSFGFSKKDISMQGLVFFSDLKILLYPVFTMQGTKWITSEGLLKEAGVLDSTKENNVNELVYYVITNEDDKVGHINLGWLYFPYKKVETNIDLSLLEGAISLSDIVIVPENLISQIINSNLEVRTSVSINPITGAAKEGALFTSEAIPRGTIFYGEIRFFDRSKLIEDLPLKEFVFKMLEDSKKYYETLGVGGMTTRGFGRMKVLTKLNKSNGGDSNEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGW8</accession>
    <name>B7IGW8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Chain length determinant protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_872</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579847.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_872"/>
    <dbReference type="EnsemblBacteria" id="ACJ75332">
        <property type="protein sequence ID" value="ACJ75332"/>
        <property type="gene ID" value="THA_872"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_872"/>
    <dbReference type="eggNOG" id="COG0489">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009912_2_1_0"/>
    <dbReference type="OMA" id="MRDHDNA"/>
    <dbReference type="OrthoDB" id="1184929at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009103">
        <property type="term" value="P:lipopolysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032807">
        <property type="entry name" value="GNVR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003856">
        <property type="entry name" value="LPS_length_determ_N_term"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13807">
        <property type="entry name" value="GNVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02706">
        <property type="entry name" value="Wzz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Wzz" evidence="8">
        <location>
            <begin position="7"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="GNVR" evidence="9">
        <location>
            <begin position="382"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="280"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="660" mass="74800" checksum="517E270B8707929D" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGY7</accession>
    <name>B7IGY7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_892</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579866.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_892"/>
    <dbReference type="EnsemblBacteria" id="ACJ75351">
        <property type="protein sequence ID" value="ACJ75351"/>
        <property type="gene ID" value="THA_892"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_892"/>
    <dbReference type="eggNOG" id="ENOG502ZXCS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190044_0_0_0"/>
    <dbReference type="OMA" id="MCESAQI"/>
    <dbReference type="OrthoDB" id="1988233at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012454">
        <property type="entry name" value="DUF1659"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07872">
        <property type="entry name" value="DUF1659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="63" mass="7093" checksum="2012E7FDB2152697" modified="2009-02-10" version="1">MKKLSIKWNVGTDENGEPILKTQSLNVQDSVDAQKALLIAQTIEKYTNYSLYSAQLVTYTPVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGZ3</accession>
    <name>B7IGZ3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100759.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_898"/>
    <dbReference type="EnsemblBacteria" id="ACJ75357">
        <property type="protein sequence ID" value="ACJ75357"/>
        <property type="gene ID" value="THA_898"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_898"/>
    <dbReference type="eggNOG" id="COG0613">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067347_1_0_0"/>
    <dbReference type="OMA" id="CTWGGAT"/>
    <dbReference type="OrthoDB" id="1309717at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POLIIIAc" evidence="1">
        <location>
            <begin position="3"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="266" mass="30604" checksum="D2EC52B6D9F748E0" modified="2009-02-10" version="1">MLVDFHMHSTASDGTFNPNELVTLVKERKIECFSLTDHDTIDGVKQIKEKNFIPGVEISIEHPTTLHILGYGFDINNEKFNKVLDQLKQYRYERNVKMVEKANALGFDITLEELLEEANGTLIGRPHFASLLLKKGYVKDRKEAFEKYLKRGMPLYEDKKRLDLENAIEIITQAGGIAVFAHPYQTSEDEDEIEKLLKKMVDLGLKGIEVYYSKHTKGMIETYEKLAKKYNLVKTAGSDFHGQNTPDIELGIEVRRENIKEFLSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGZ9</accession>
    <name>B7IGZ9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved protein, with A weak D-galactarate dehydratase/altronate hydrolase domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579873.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_904"/>
    <dbReference type="EnsemblBacteria" id="ACJ75363">
        <property type="protein sequence ID" value="ACJ75363"/>
        <property type="gene ID" value="THA_904"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_904"/>
    <dbReference type="eggNOG" id="COG1672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021114_0_1_0"/>
    <dbReference type="OMA" id="HYQTIFY"/>
    <dbReference type="OrthoDB" id="191489at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018631">
        <property type="entry name" value="AAA-ATPase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012547">
        <property type="entry name" value="PDDEXK_9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09820">
        <property type="entry name" value="AAA-ATPase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08011">
        <property type="entry name" value="PDDEXK_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA-ATPase_like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="518" mass="60836" checksum="A506E144921D0D16" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH05</accession>
    <name>B7IH05_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flagella basal body P-ring formation protein FlgA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">flgA</name>
        <name evidence="2" type="ordered locus">THA_910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100784.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_910"/>
    <dbReference type="EnsemblBacteria" id="ACJ75369">
        <property type="protein sequence ID" value="ACJ75369"/>
        <property type="gene ID" value="THA_910"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_910"/>
    <dbReference type="eggNOG" id="COG1261">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_896959_0_0_0"/>
    <dbReference type="OMA" id="GPMLKVV"/>
    <dbReference type="OrthoDB" id="1169937at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017585">
        <property type="entry name" value="Flag_basal_body_FlgA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039246">
        <property type="entry name" value="Flagellar_FlgA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36307">
        <property type="entry name" value="PTHR36307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13144">
        <property type="entry name" value="ChapFlgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03170">
        <property type="entry name" value="flgA_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChapFlgA" evidence="1">
        <location>
            <begin position="185"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="310" mass="34281" checksum="75FC5B1B1528BE87" modified="2009-02-10" version="1">MRKGILFILLLPILSLSMYIQFLDVATVTRNEVTVFDVAATYTQIDATSLQNIVLAYLPENSSTDINIKYILQRLSRVATQVEATPTVGVVKVVFPNLLTRESSMSTLTVEQALKIATEVALSMLPINSTVEVTSTYGKIVEHDNFDYDVIQSVGGSFLVRFALKKDSRTVGYFNVNLIGKCIRKIPVAARNINYGEQVNMDDVAFADVNIFSLRGTPLDVSKLPMISRKYFKVGEPLFEEYFERVPDVVVGQVIVGYVELPGVIVKTMLRALESGNVGDVIKARNIESGKIVYGLIVEGPMLKVVEVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH33</accession>
    <name>B7IH33_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transporter, major facilitator family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_938</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100840.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_938"/>
    <dbReference type="EnsemblBacteria" id="ACJ75397">
        <property type="protein sequence ID" value="ACJ75397"/>
        <property type="gene ID" value="THA_938"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_938"/>
    <dbReference type="eggNOG" id="COG0738">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061779_0_0_0"/>
    <dbReference type="OMA" id="FWFTISF"/>
    <dbReference type="OrthoDB" id="1121832at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="1"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="361" mass="40748" checksum="E1C37376072E1EC9" modified="2009-02-10" version="1">MVFIFLNIFLGALLANSIAPLMPVFQEKLNATVAFSTFIPVVNTAGTTIFSFIISFFINKLGLKKTHFIGHLFLLSSLLILSFLESLKFILLGVFLLGAGLSIIFTSSTTFLSHFKDPKFGIVHGFFGLGGILSPMFVSLFLKSGISYKYLYLIYLSFVIFLFIWNMKMKFPDIKINEGSFSSSKPYLKPLFYITVLSLFFYSASEISTATWAPNLFLNFGFTKQQAALFLSIFWGLFTFSRFLMDFLLKFVNYRLYVLTITSLSFIFLSLLIVLKIPFLFYLFGLSMGAIFPLIQRNANIKLDKSEVGFLNGITYSATGIGSLLFISLMGYISKFSIRAMFVVPIIGLAVVFLLQKNLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH44</accession>
    <name>B7IH44_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-amylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_949</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579895.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_949"/>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75408">
        <property type="protein sequence ID" value="ACJ75408"/>
        <property type="gene ID" value="THA_949"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_949"/>
    <dbReference type="eggNOG" id="COG0366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006462_2_0_0"/>
    <dbReference type="OMA" id="FPFIDSH"/>
    <dbReference type="OrthoDB" id="1573900at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006046">
        <property type="entry name" value="Alpha_amylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00110">
        <property type="entry name" value="ALPHAAMYLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="3">
        <location>
            <begin position="472"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="976" mass="112953" checksum="F571F6E33D63CCBB" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH62</accession>
    <name>B7IH62_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2344 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_967</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100930.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_967"/>
    <dbReference type="EnsemblBacteria" id="ACJ75426">
        <property type="protein sequence ID" value="ACJ75426"/>
        <property type="gene ID" value="THA_967"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_967"/>
    <dbReference type="eggNOG" id="COG5011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1304019_0_0_0"/>
    <dbReference type="OMA" id="TEGFHPK"/>
    <dbReference type="OrthoDB" id="1514287at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018768">
        <property type="entry name" value="DUF2344"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10105">
        <property type="entry name" value="DUF2344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03936">
        <property type="entry name" value="sam_1_link_chp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2344" evidence="1">
        <location>
            <begin position="5"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="200" mass="23616" checksum="FCF529AC1337A204" modified="2009-02-10" version="1">MSYSYLVRFKKLGPYRFVAGLDTITMIERTFRRTNLKLTFTEGFHPKPKFSYIDPVSTGIIDLAFYVNINLEEDYDPQVVYNEITAVQPLHLKVDSVTKNFLNLSKIEKYEFLVFVKKPFDFDATKVIEKKTKRGIRQISLDVFENVRKFEKKDYVVINYIIRKENTFNPYLFSDKVFLAIRKEAYIQDNALSLLFERGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH63</accession>
    <name>B7IH63_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_968</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100932.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_968"/>
    <dbReference type="EnsemblBacteria" id="ACJ75427">
        <property type="protein sequence ID" value="ACJ75427"/>
        <property type="gene ID" value="THA_968"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_968"/>
    <dbReference type="eggNOG" id="COG3391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1137588_0_0_0"/>
    <dbReference type="OMA" id="PAVVYKC"/>
    <dbReference type="OrthoDB" id="1352758at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="247" mass="28349" checksum="A902C5F445E46B12" modified="2009-02-10" version="1">MKIILSLIFSIWGLFAPESVVVDNSGGYFISNMGKLYVKDGNVLYQSKEKNEYILSLVDPRGLYLSGKDLWIVDFDRLIKYDLSSKTYKSYFATFPRYLNDVTMYEGKVYVTDTYGNSVYILENDKLNVVFNILRPNGITTDGKYLYVISFENPAVVYKCSKEGVVESFTLSDVKGGDGIFFADDLFFVSGYNSKNVVVYDKDWNKLLEKKGFSSPSDLYYKDGYLYVPDMKDGKIYVFQVKKEKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH99</accession>
    <name>B7IH99_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ggdef family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579924.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1004"/>
    <dbReference type="EnsemblBacteria" id="ACJ75463">
        <property type="protein sequence ID" value="ACJ75463"/>
        <property type="gene ID" value="THA_1004"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1004"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_32_0"/>
    <dbReference type="OMA" id="NRHFIEE"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="177"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="299" mass="35532" checksum="B57AF13923186C2E" modified="2009-02-10" version="1">MKENKIEAFFEKLMIKTRPHLKFDGWSVIEYDEINGPSFISLSRKYKKYDLESIERKISNSQSTLFDTVLKTRNWKYVADPFKLNVWMPGSRIRSWIGVPLMFDNEVFGVLNIDYFKPKRLTFKDKLFLNSFQKNFSIYASNFKELKSLFLQKYIDHLTGLKNRHFIEEYFEKNNRDRIAIIFCDLNKFKAVNDTYGHRIGDEVIKIVARRIRNILKSTDEIVRYGGDEFIVLTKNIENVETIKLRIKNAIKKYDIIIEGKRIKIGISCGHAIYPDESTDFWEIMHLADLRMYSEKGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHB2</accession>
    <name>B7IHB2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide transport ATP-binding protein OppD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1018</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579936.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1018"/>
    <dbReference type="EnsemblBacteria" id="ACJ75476">
        <property type="protein sequence ID" value="ACJ75476"/>
        <property type="gene ID" value="THA_1018"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1018"/>
    <dbReference type="eggNOG" id="COG0444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_0"/>
    <dbReference type="OMA" id="LEIPQGC"/>
    <dbReference type="OrthoDB" id="1101128at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="5"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="319" mass="36444" checksum="F011AD4CB294BB3B" modified="2009-02-10" version="1">MFFEAKNLKIYYKTKKGFVKAVDNISLNIEKGQTVGLVGESGCGKSTFGQGLLKILPPTTYYDGSVKIENQELINLTEDKMRKIRGKKIGMIFQDPMTSLNPVMKIEKLFYETLKTHEPEITKEEARKRTAKVLEKVGIEPERMKEYSFQFSGGMRQRVMIALTLVLNPILVIADEPTTSLDVIVQAQIMKLLNELRKEYDMSMILITHDLGVVAEMADKICVMYGGHIVEEAKSEDLYYKPKHPYTKLLLQSIPNTNINDLDLKYIYGSPPDLSNPPIGCRFVDRCPYKKEICKLKEPPVVEVDESKVKCWLYSEVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHC7</accession>
    <name>B7IHC7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579946.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1033"/>
    <dbReference type="EnsemblBacteria" id="ACJ75491">
        <property type="protein sequence ID" value="ACJ75491"/>
        <property type="gene ID" value="THA_1033"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1033"/>
    <dbReference type="eggNOG" id="ENOG5033TGH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_600968_0_0_0"/>
    <dbReference type="OMA" id="LAIDINY"/>
    <dbReference type="OrthoDB" id="435984at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="454" mass="52749" checksum="E38F294EBC7DC8DE" modified="2009-02-10" version="1">MRKIFSLVLILVSLFAFSLKIYPNLIYNIEVKDLIFEYSFFVRNDLNYDAIIRIEKIDFVTDGKKYEFDLPDYKYSVKNYVDLDSYEFELKPDEEKRITLKFNIPSDFDGASGVFALKISQESKRGGQIQIRLNYIVPFFLRFKNINPIQKVDITDVDIRNLNFYLDERYGNFGTLITMKLKNNGNVVFLPYGTIKITSVDLRTTISEKEINSFDLVVFPENEAFYTFKIPEILPTGKVRFSVEGESYGMRFSISKDATINETYENLIVEASPNVIIFDDKTINTLKSILIKNLTSFKSEISWEIVDQKGLRSDIFKLYPSVLRIYPYRATSIKIRSNEREITNVGDRIFYLHLLYDGKKISVKSPLIVVRGQKLSPELKAKYLPEENSIELTNTGNCLLAIDINYSSFSLYNDIVLIPGQSKKIKLEDNISISRIILKYGIFGENKEYEEYIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHD1</accession>
    <name>B7IHD1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101070.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1037"/>
    <dbReference type="EnsemblBacteria" id="ACJ75495">
        <property type="protein sequence ID" value="ACJ75495"/>
        <property type="gene ID" value="THA_1037"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1037"/>
    <dbReference type="eggNOG" id="ENOG5034228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1721478_0_0_0"/>
    <dbReference type="OMA" id="TQAFAID"/>
    <dbReference type="OrthoDB" id="1787056at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857421" evidence="1">
        <location>
            <begin position="19"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="152" mass="16535" checksum="1F7CB64653BC58C6" modified="2009-02-10" version="1">MKKVVILLIALLSLAAFAAGTANIKLNPNGNWTQTLTFSVSQWVKVTWDYDETQVFAVDDTTGIANVGNIRFESNKTFKMYYQTTIVADGGVSDLAVNEVKVGSTILSNDNTNPTSVSTKILEGNLAITFGGDYTNASDDFTVKFDFTFLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHD4</accession>
    <name>B7IHD4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1040"/>
    <dbReference type="EnsemblBacteria" id="ACJ75498">
        <property type="protein sequence ID" value="ACJ75498"/>
        <property type="gene ID" value="THA_1040"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1040"/>
    <dbReference type="eggNOG" id="COG0079">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1414561_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="192" mass="22804" checksum="CF2C19F28AB75243" modified="2009-02-10" version="1">MLILGNPNNPTGIYKDLEEFFKLNQNIEIVLDEAFIDFVENPKDYEKFDNVLLIRTFTKFFGIPGIRVGYVKTKKYQEIFKKYRMSWAIGGMGYTFLNTLLKNIETVNSFKETTISFLKEQKERFKEFIYTKSDTNYFLVDVSDIDNFLNFCHSRKIHVRDARNFGLNLVRIGLKDKKSNEKLFNTLRKWRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHE4</accession>
    <name>B7IHE4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1051</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579959.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75508">
        <property type="protein sequence ID" value="ACJ75508"/>
        <property type="gene ID" value="THA_1051"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1051"/>
    <dbReference type="eggNOG" id="COG4277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033784_0_0_0"/>
    <dbReference type="OMA" id="GYIHLKA"/>
    <dbReference type="OrthoDB" id="940969at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023874">
        <property type="entry name" value="DNA_rSAM_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21180:SF9">
        <property type="entry name" value="PTHR21180:SF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01102">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03916">
        <property type="entry name" value="rSAM_link_UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="37"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="386" mass="44894" checksum="8F9FC39BE083286B" modified="2009-02-10" version="1">MTLEEKLSILSATAKYDVSCSFGERKKDFVYYSVANGRYVPILKILFSNACIYDCAYCINRKSNNIKRASFTVDEVVKLTVDFYKRNYIEGLFLSSAIIKDSNYTMEQLIKVAKKLREEEKFPGYIHLKIVPGADEKLVEKAGMYADRLSINVEFLKKDAFSILAPEKKPEAIQKPLQTSSIKYLQYIEEKKKYSHVKPYSPLGQTTQIIVGATNESDKKIIEFSSKLYKIYKLKRVYYSGYIAINKDKRLPQRSENSLREHRLYQTDFLIRFYNYSIEEIFDNCENLDLNIDPKSLWALKHPEFFPIDISRATFDELIRIPGIGLKSAKKIIMLRKHGILNFDTLKKTGISLKKAKNFITIKGKNFKDSKIYMPLFEFSQNGWDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHH9</accession>
    <name>B7IHH9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Iron-sulfur flavoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579992.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1087"/>
    <dbReference type="EnsemblBacteria" id="ACJ75543">
        <property type="protein sequence ID" value="ACJ75543"/>
        <property type="gene ID" value="THA_1087"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1087"/>
    <dbReference type="eggNOG" id="COG0655">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050993_1_0_0"/>
    <dbReference type="OMA" id="SPVYVYH"/>
    <dbReference type="OrthoDB" id="796211at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="3"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="233" mass="27076" checksum="5303748A13A11B1C" modified="2009-02-10" version="1">MKKVLVINGSARKGRTFELLKNIAEKLPYDVEYIHLKEYDIKDCLGCEVCKKKDFCVIKDQAEELMEKLKSADGIVLGTPVYIENVSGVLKKFLDRTCRWYHRPPLLGKPALLVATTAGSSLKKVLQYLEDVVTTWGMIPTGKIGKRVSEKRVVSKKQIKKFINAMEKPPKRYIRLTKLIRFQVQKAMATNLLDIDKNYWIEKGLNKKKLPLQILYRSFLILIFNFIWKIHFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHJ4</accession>
    <name>B7IHJ4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ornithine carbamoyltransferase</fullName>
            <ecNumber evidence="3">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">argF</name>
        <name evidence="9" type="ordered locus">THA_1105</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>carbamoyl phosphate + L-ornithine = H(+) + L-citrulline + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101204.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1105"/>
    <dbReference type="EnsemblBacteria" id="ACJ75558">
        <property type="protein sequence ID" value="ACJ75558"/>
        <property type="gene ID" value="THA_1105"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1105"/>
    <dbReference type="eggNOG" id="COG0078">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_3_1_0"/>
    <dbReference type="OMA" id="DGNNVCN"/>
    <dbReference type="OrthoDB" id="988597at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="OTCace_N" evidence="8">
        <location>
            <begin position="8"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="OTCace" evidence="7">
        <location>
            <begin position="154"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="5">
        <location>
            <begin position="57"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="5">
        <location>
            <begin position="133"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Ornithine binding" evidence="5">
        <location>
            <begin position="233"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="5">
        <location>
            <begin position="270"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="326" mass="36822" checksum="21FB92D2D36DEE36" modified="2009-02-10" version="1">MPVNLKGRSLLTLKDFTAEEIKYLLNLAFDLKAKKRAGIRQELLKGKNIVLIFDKTSTRTRTAFEVAAYDEGAHVTFLTNSQMGKKESIEDTAKVLGRMYDGIEYRGYKQEVVEALAKYSGVPVWNGLTDEAHPTQGLADVMTMMEFIHKPLNQMKLVFVGDTRNNVANTLMRISSKLGIHYVAVGPEQLKPSDEIMNEALENAKESGAIIEYTPDLDGVKGADAIYTDVWVSMGEEDKMPERVELLTPYRVDMDLIKRTENPNVIFLHCLPSFHDFETTVAKEAKERGLDIREVTDEVFRSKHSKVFDQAENRMHTIKAVMVATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHK8</accession>
    <name>B7IHK8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101223.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1119"/>
    <dbReference type="EnsemblBacteria" id="ACJ75572">
        <property type="protein sequence ID" value="ACJ75572"/>
        <property type="gene ID" value="THA_1119"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1119"/>
    <dbReference type="eggNOG" id="COG2345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1517224_0_0_0"/>
    <dbReference type="OMA" id="HTFTVQR"/>
    <dbReference type="OrthoDB" id="1720273at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="177" mass="20495" checksum="E1D371907ABF40F6" modified="2009-02-10" version="1">MNELLKVISSPQLFEVLNFLKNNPNMNPSTIAKKLGYHTFTVQRYLEIMEKFNIVSFKEERKVGRPSKKYRYIGGKITIDIDEILNIFNLKGKRVREKAGDFKYSYDLNKETIKGVILNNKKIKFNEVEGKILFLIPPVNSNGITVCEISNKISINEFDILFAIKKFLSLDLIEVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHL1</accession>
    <name>B7IHL1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580014.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1122"/>
    <dbReference type="EnsemblBacteria" id="ACJ75575">
        <property type="protein sequence ID" value="ACJ75575"/>
        <property type="gene ID" value="THA_1122"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1122"/>
    <dbReference type="eggNOG" id="ENOG5032VX1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_529708_0_0_0"/>
    <dbReference type="OMA" id="WAFKRIV"/>
    <dbReference type="OrthoDB" id="1201330at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018646">
        <property type="entry name" value="12TM_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09847">
        <property type="entry name" value="12TM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="394"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="498" mass="56510" checksum="9EB6EA6F50CAD58E" modified="2009-02-10" version="1">MRDFIILLKYLSNPYMNTSKSKKSSVSKNFSKQLLGYAIGSIPLGIFVFVFSKQIFEKLYMVDPAASRLMFLFWVSILSLFFIVGFIGLAMYSLSRNDELEILLTMPISRTTLTVFQIFTATISQIYVLVFFVFVTLAYLIAAKGNILIGIVQLIVHLAFLLLLSSTIAILIGGKTSKSFTRRFYMIIVLLSIFFYFFIIALVDVDVAELQNLVKMFLFSTKEYNILAWSFISTKTLIFSIISAAILFILFRIISSKIGFEPIQRNSKKRYDKKGSGSVLNAIIKKDLKATIRYEQFLYFVLYPIGFGIFMMFLNKDILSPLMFTIPIFTFYIALETGILMISEISKIEYVSVFPVKFSTLVFSKIAIPVLINIILLLIIFIVSSIFYSLNLLVLLGLLFALLLFTMSSILGAYFAISSSNLKIVNMNRIFSISQTLILEAVTMGLAFGSIIPLGILIQQQSLEWWGWLIMISSLATIVLISILFFGKLKKKMEVRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHM1</accession>
    <name>B7IHM1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-splicing ligase RtcB</fullName>
            <ecNumber evidence="4">6.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rtcB</name>
        <name evidence="5" type="ordered locus">THA_1132</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3 4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="3 4">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RtcB family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1132"/>
    <dbReference type="EnsemblBacteria" id="ACJ75585">
        <property type="protein sequence ID" value="ACJ75585"/>
        <property type="gene ID" value="THA_1132"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1132"/>
    <dbReference type="eggNOG" id="COG1690">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022279_0_1_0"/>
    <dbReference type="OMA" id="QTRGVEC"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008452">
        <property type="term" value="F:RNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="PTHR11118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="SSF103365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01288">
        <property type="entry name" value="UPF0027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 4" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="GMP-histidine intermediate" evidence="1">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="184"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="299"/>
            <end position="300"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="344"/>
            <end position="347"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="368"/>
            <end position="371"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU371113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="445" mass="49663" checksum="4AB0C336582875B3" modified="2009-02-10" version="1">MYIHKIKKTGNMKVDAFVLTDNISEISEAIEQLKNVASLPTIVKAAYAMPDIHWGYGFPIGGVAAFSNIISPGGVGFDINCGVRLLKTQIDYNDFKKYIENTIKELYDVIPVGVGATSKKFDKKSFKKIIEEGAKRAIEMGCGLSEDLEYIEDFGSISPASMEDISKTAYERGREELGTLGAGNHFIEIQKVEEIYDEKKANILGLYKNHIVIMIHTGSRGFGHQIATDYIKLMRNLHNDLPDKQLAYAYFDSDLGQKYYSAMNCAANYAFSNRQIITHYIRKVFSKFSKVEIVYDVAHNIAKVEKHDNQKLIVHRKGATRAFGPYHPDIPEKYKSIGQPVLIPGNMGTASYVMVGTNENFAFSSTAHGAGRVLGRRQALKKLNLNQVLSNLQKRGIIVKSKSKKTILEEAPDAYKEIDRVVEIVDKLNLSKKVAKLIPLGVIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHN5</accession>
    <name>B7IHN5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromosome partition protein Smc</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">smc</name>
        <name evidence="5" type="ordered locus">THA_1147</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for chromosome condensation and partitioning.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">Contains large globular domains required for ATP hydrolysis at each terminus and a third globular domain forming a flexible hinge near the middle of the molecule. These domains are separated by coiled-coil structures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580035.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1147"/>
    <dbReference type="EnsemblBacteria" id="ACJ75599">
        <property type="protein sequence ID" value="ACJ75599"/>
        <property type="gene ID" value="THA_1147"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1147"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001042_2_2_0"/>
    <dbReference type="OMA" id="HNKIAME"/>
    <dbReference type="OrthoDB" id="1149850at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01894">
        <property type="entry name" value="Smc_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011890">
        <property type="entry name" value="SMC_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="SSF75553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02168">
        <property type="entry name" value="SMC_prok_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="4">
        <location>
            <begin position="512"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="165"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="661"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="1155" mass="134086" checksum="5313585558C36627" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHP5</accession>
    <name>B7IHP5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580045.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1157"/>
    <dbReference type="EnsemblBacteria" id="ACJ75609">
        <property type="protein sequence ID" value="ACJ75609"/>
        <property type="gene ID" value="THA_1157"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1157"/>
    <dbReference type="eggNOG" id="ENOG50337N2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1785992_0_0_0"/>
    <dbReference type="OrthoDB" id="1965555at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="151" mass="17223" checksum="5066FDF506048FE6" modified="2009-02-10" version="1">MKKGIINISLGIILIFFSALSALLLSYLFKDAINVEKKIIETVDNTYSKILYISGANIWRKFLTENAAYWDDIQAEFEDASISGNSTRTQLVLNTYLEYNLGSKKKYDYTLVSTTTTYIFEEKYINVIFEILKNGIHVSKGVSFDFGWPGI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHV3</accession>
    <name>RL16_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplP</name>
        <name type="ordered locus">THA_1222</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101449.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHV3"/>
    <dbReference type="SMR" id="B7IHV3"/>
    <dbReference type="STRING" id="484019.THA_1222"/>
    <dbReference type="EnsemblBacteria" id="ACJ75667">
        <property type="protein sequence ID" value="ACJ75667"/>
        <property type="gene ID" value="THA_1222"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1222"/>
    <dbReference type="eggNOG" id="COG0197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078858_2_1_0"/>
    <dbReference type="OMA" id="KGAVEYW"/>
    <dbReference type="OrthoDB" id="1786618at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01342">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_L10e/L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_L10e/L16_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020798">
        <property type="entry name" value="Ribosomal_L16_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="PTHR12220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00060">
        <property type="entry name" value="RIBOSOMALL16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="SSF54686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01164">
        <property type="entry name" value="rplP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00586">
        <property type="entry name" value="RIBOSOMAL_L16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_1000143040" description="50S ribosomal protein L16">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01342"/>
        </source>
    </evidence>
    <sequence length="142" mass="15896" checksum="8A4D97B9E12276C9" modified="2009-02-10" version="1">MLMPKRVKYRKQHRGRMKGNAKGGALVNFGEYGLKAMESHWITAQQIEACRIAITRTLKKTGKLWIRVFPDKSYTKHPAESKLGKGKGNVEGWVAVVKPGKILFEIGGVDEKLAREALEYAATKLPIRTKIVKRHEIGGEAV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHX0</accession>
    <name>RS13_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsM</name>
        <name type="ordered locus">THA_1239</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101491.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHX0"/>
    <dbReference type="SMR" id="B7IHX0"/>
    <dbReference type="STRING" id="484019.THA_1239"/>
    <dbReference type="EnsemblBacteria" id="ACJ75684">
        <property type="protein sequence ID" value="ACJ75684"/>
        <property type="gene ID" value="THA_1239"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1239"/>
    <dbReference type="eggNOG" id="COG0099">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103849_1_2_0"/>
    <dbReference type="OMA" id="YRGLRHK"/>
    <dbReference type="OrthoDB" id="1772647at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_S13_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="30s_Rbsml_prot_S13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_S13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019980">
        <property type="entry name" value="Ribosomal_S13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_S13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF1">
        <property type="entry name" value="PTHR10871:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03631">
        <property type="entry name" value="uS13_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_1000141320" description="30S ribosomal protein S13">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="122" mass="14094" checksum="9CFE7C35C600AC94" modified="2009-02-10" version="1">MARIVGVEIPNDKKVEIALTYIYGIGRTRAKQICEATNIDPDKRVRELNDEEISKIATYIQQNFKVEGELRTEVMQNIKRLIDIGCYRGFRHKLGLPVRGQKTKSNARTRKGPRPSRIKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHY3</accession>
    <name>B7IHY3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endoribonuclease Cas2</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cas2</name>
        <name evidence="10" type="ordered locus">THA_1252</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas1 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580090.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1252"/>
    <dbReference type="EnsemblBacteria" id="ACJ75697">
        <property type="protein sequence ID" value="ACJ75697"/>
        <property type="gene ID" value="THA_1252"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1252"/>
    <dbReference type="eggNOG" id="COG1343">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161124_0_1_0"/>
    <dbReference type="OMA" id="LTHVQNS"/>
    <dbReference type="OrthoDB" id="1951170at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09725">
        <property type="entry name" value="Cas2_I_II_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405">
        <property type="entry name" value="PTHR34405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="87" mass="10187" checksum="EBA2BB249D1DC9FF" modified="2009-02-10" version="1">MFVILVYDIGEKRVNKVLKKCRQYLTWVQNSVFEGEITKGGLKKLKMELGRIIEKKEDSVIIYAFENTRYSNREIIGLEKNSLGIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHZ6</accession>
    <name>B7IHZ6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580103.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1265"/>
    <dbReference type="EnsemblBacteria" id="ACJ75710">
        <property type="protein sequence ID" value="ACJ75710"/>
        <property type="gene ID" value="THA_1265"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1265"/>
    <dbReference type="eggNOG" id="COG4267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043533_1_0_0"/>
    <dbReference type="OMA" id="ITQFQVS"/>
    <dbReference type="OrthoDB" id="859701at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031617">
        <property type="entry name" value="PelG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16933">
        <property type="entry name" value="PelG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="466" mass="53591" checksum="F3169DBCABF64212" modified="2009-02-10" version="1">MAGVGFKLNKLFYKNQISTDVLAVIYSILTSSGPWIITTISLWVVIYSLKSADIYFNMAVIYAFILSIIFSGIFSMFLSRRTSDLIYSKKYEKILPETLSIILVNNLIVITYLIIFFLIFKHDIKFILSFSYLTASLTTLWLISVSSLATDSINWYIFSYLTMGISSIILAKFFGIIGYAIAVNIGIIINVFVVIYYFGSSSKKISFEWLKEVKNYWQNLLIGFTYYLSIWIDDIIVWNHPKFGEEPISGFKFSYVYDSPMFFSYLTIIPTITMFILVLETRFYKKYKSFYNSLIEGYVLSEIILLKNSMEKELKQNISLTIKIQALITTVFFILNELELLPFSNQLSKPILRLGLIGAMLNGFYLMMLLLILYFDFRNLALAINLSVLGLNSILSTIFVNKIGYAALGSGYAFSFAIGTFISYYLLKKRVNKIIQIEYSRQKVDLKEGYYLRLSQLPTAYRSGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMU3</accession>
    <name>C0QMU3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_2017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580546.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2017"/>
    <dbReference type="EnsemblBacteria" id="ACN29510">
        <property type="protein sequence ID" value="ACN29510"/>
        <property type="gene ID" value="THA_2017"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2017"/>
    <dbReference type="eggNOG" id="COG3547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036902_4_1_0"/>
    <dbReference type="OMA" id="WFFAFLD"/>
    <dbReference type="OrthoDB" id="655500at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transposase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEDD_Tnp_IS110" evidence="2">
        <location>
            <begin position="20"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="258"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACN29510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="419" mass="48859" checksum="599715962B1CD2A4" modified="2009-05-05" version="1">MKQKSINKKISRITQDTLIVGIDVAKRTHWIRMMDFRGIDLIKPFKINNTMDGIKMLEEKIKSVKEENKLNKVVLGMEPSGHYWKVLAWQMKISKEIDYLVGVNPYHVKKSKEFDDNSPGKSDRKDAGVIARLIRDGRYFDIYLPEDIYGELRVITTTREQLVNKRKNAKNMVISVMDEYFPEYEKIYKNIFSEGSIKLLKTHPFPKEIIEVGIEEIEKVLKKSTNGKDWKSRAQKIYESAKKSIGVREGQKSAKIKLRMLLEEIEFLTSQIEELEKEMEKLVEETEEGEYIKSVPGIGAIMTAIILGEIGDINRFKSWKEIRKLAGLNLYEVSSGEHKGKTRITKRGRPLLRKIIYLMAQTTLKHNHEIRKKYEQLRRREKNPLKAVQALIAIGLKMIRIVFKLAKSKIKYEPEKVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICQ6</accession>
    <name>B7ICQ6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sensory box sensor histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1338</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1338"/>
    <dbReference type="EnsemblBacteria" id="ACJ75783">
        <property type="protein sequence ID" value="ACJ75783"/>
        <property type="gene ID" value="THA_1338"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1338"/>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_39_0"/>
    <dbReference type="OMA" id="CAYLMKP"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="60"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="10"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="280" mass="31417" checksum="83C038E84D5015AB" modified="2009-02-10" version="1">MVITSLYFKNVEKTKKLLELNEELSEANEELEQTIRELEQTQESLIISEKMAALGKLMLNIAHDINTPIGIIFTASTEIENILESNRTDKETLKQLSKIIIKNSQKIAEIISSLKKTAIHEVSDNFSKFNIKELVEDIVDTLSLNLKKNNIKTIIDVDDKNIFSNPGAIAQILMNLISNTVDHAFKDEKSEKIISISAEVNKKHLKLIVSDNGVGIDEKIQRKIFDPFFTTDKEKGTGLGLSIVHQLVVKLLKGHIEVKSDKGKGTTFIIIIPLKEENNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICR1</accession>
    <name>B7ICR1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidohydrolase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1343</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580161.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1343"/>
    <dbReference type="EnsemblBacteria" id="ACJ75788">
        <property type="protein sequence ID" value="ACJ75788"/>
        <property type="gene ID" value="THA_1343"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1343"/>
    <dbReference type="eggNOG" id="COG1228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046987_0_0_0"/>
    <dbReference type="OMA" id="HCTEGHK"/>
    <dbReference type="OrthoDB" id="1456605at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="1">
        <location>
            <begin position="49"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="379" mass="42390" checksum="E8366C49FF2DE4B5" modified="2009-02-10" version="1">MILIKNGTIYPITKSPFKGDILIENGKIKKIGENIEEKGAEVIDATGKYIFPGFIDAHSHIGVFEEGVGEFYYQDGNEYSNPLTPHVRALDAFYPGDKAIKRALSGGVTTVMVVPGSANPIGGQGFILKFKSDIVDEMVIRQPAGLKMAFGENPKRVYGSQNKTPMTRLGVAAIIREYFTKVKDYMRRRQEDPKTPIDFTLEIGVKVLKKEIPARCHAHRADDIVTAIRIAEEFNFDLVIEHATEGYKIADFLKKKKIPVVLGPLFGFRTKLELTDMTYEAIKIINEKGILAALMCDHPVIHLEHANIQAATALRYGAKEEDLLKMLTINPAKILKIDDRVGSIEEGKDADIVIWNTHPFDFKARAEKVFIEGKLVYND</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICS3</accession>
    <name>B7ICS3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Endoribonuclease L-PSP, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580172.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1355"/>
    <dbReference type="EnsemblBacteria" id="ACJ75800">
        <property type="protein sequence ID" value="ACJ75800"/>
        <property type="gene ID" value="THA_1355"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1355"/>
    <dbReference type="eggNOG" id="COG0251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100715_7_3_0"/>
    <dbReference type="OMA" id="GSYFKEP"/>
    <dbReference type="OrthoDB" id="1850770at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006056">
        <property type="entry name" value="RidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019897">
        <property type="entry name" value="RidA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="PTHR11803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="SSF55298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00004">
        <property type="entry name" value="TIGR00004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01094">
        <property type="entry name" value="UPF0076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="124" mass="13619" checksum="56E11BE90D5B2100" modified="2009-02-10" version="1">MEFINPSNAPKAVGPYSIAVKSNGFVFVSGQLPIVVETGELIKGDIKRATEVIIKNMENILKEAGSSLGKLVKVNVYIRDMSKFSEFNEIYERLLNGHKPARAVVEVSNLPKDSDIEIEAIAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICU0</accession>
    <name>B7ICU0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1372</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101748.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1372"/>
    <dbReference type="EnsemblBacteria" id="ACJ75817">
        <property type="protein sequence ID" value="ACJ75817"/>
        <property type="gene ID" value="THA_1372"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1372"/>
    <dbReference type="eggNOG" id="COG1180">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044176_1_0_0"/>
    <dbReference type="OMA" id="YHFYPGS"/>
    <dbReference type="OrthoDB" id="1141206at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027596">
        <property type="entry name" value="AmmeMemoSam_rS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PTHR30352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01101">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04337">
        <property type="entry name" value="AmmeMemoSam_rS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="67"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="327" mass="37188" checksum="FA5E47CCD54843F0" modified="2009-02-10" version="1">MKKMATFFDEGDKGIVCRLCPRHCFLSDGQIGFCKTRKNENGILYTINYGEISSIAMDPIEKKPLFHFRPGSKILSIGSWGCNFSCGFCQNWEISQVKPAVKTVTPSQIVEIAKTRNSNGIAYTYNEPIVSYEFVLDTSRIAHKEGLYNVLVTNGYIEFEPLNVLLQSIDAMNIDLKGSNEEFYKEMGGSYSHVLKVIETAKNRGVHVEVTTLVIPTKNDDEKELIQEFKDLAKIDKDIPLHLSRYFPAYKYEIPPTPIETLEKLYSIAKEYLNFVYIGNVFEPRYESTFCPDCGTQLIYREGYNVDIKNITEGGYCKVCGRKVVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICV4</accession>
    <name>B7ICV4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580196.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1386"/>
    <dbReference type="EnsemblBacteria" id="ACJ75831">
        <property type="protein sequence ID" value="ACJ75831"/>
        <property type="gene ID" value="THA_1386"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1386"/>
    <dbReference type="eggNOG" id="COG3634">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082677_0_0_0"/>
    <dbReference type="OMA" id="RMAHKFA"/>
    <dbReference type="OrthoDB" id="1300544at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011903">
        <property type="entry name" value="Glutaredoxin-like_bact/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02187">
        <property type="entry name" value="GlrX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin-like_fold" evidence="1">
        <location>
            <begin position="34"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin-like_fold" evidence="1">
        <location>
            <begin position="137"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="220" mass="24875" checksum="FA8D735C32B20A64" modified="2009-02-10" version="1">MALLSAKDREYLTDLFSKELENKVKLIYFGDSKENCEYCDLEEQILDELKELSDKIIIEKYNVKENADLAEKYEVEMTPALILTLEDGEDKGVRFYGIPSGHEFGTLVQDIVTFGKGAKPELSPDTIEKLKAIDKPVKISVFVTPTCPYCPRAVLTAHNFALVNPLIKAEMIEANEFFDLSNEFGVSSVPHIVINRNPNTFFIGAYPEPQYLEEVLKAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICV6</accession>
    <name>B7ICV6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1389</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580198.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1389"/>
    <dbReference type="EnsemblBacteria" id="ACJ75833">
        <property type="protein sequence ID" value="ACJ75833"/>
        <property type="gene ID" value="THA_1389"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1389"/>
    <dbReference type="eggNOG" id="ENOG5033B2Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119456_0_0_0"/>
    <dbReference type="OMA" id="NTEEGHK"/>
    <dbReference type="OrthoDB" id="1626878at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="199" mass="22950" checksum="3407AA1CB5167941" modified="2009-02-10" version="1">MVRLNVNGNLETFESSKYKNFGELLDEIVKKYKGMVLKEVKINDKDVPINRIDELRDAILDEDLEIRVQFVPLQEFFISTLADVVDYISNIEKLLEKVSSNVLVGESEGFNNIKDLAEGISAMENLRVNSIKMTGYAPSDFENNIDEKKITEILTDFVTALETRDLIELSDLLEEKIPLVLEYYRNYFMNVLNVLKQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICW1</accession>
    <name>B7ICW1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide transport ATP-binding protein OppD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1394</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580202.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1394"/>
    <dbReference type="EnsemblBacteria" id="ACJ75838">
        <property type="protein sequence ID" value="ACJ75838"/>
        <property type="gene ID" value="THA_1394"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1394"/>
    <dbReference type="eggNOG" id="COG0444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_0"/>
    <dbReference type="OMA" id="GRQICET"/>
    <dbReference type="OrthoDB" id="1101128at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="335" mass="37764" checksum="C5A968FBAC2E28DB" modified="2009-02-10" version="1">MALLEVKDLKMHYKTKMGYVKAVDGISFTLDRGESLGIVGESGCGKTSVSMTMLRLLPENGEFRGGNVFFEDDNGKKVDLVSLPENEMRKYRWKGLAMVFQAAMNSLNPVYKVGDQIVEAILTHYPEKTIDEAKEKVAELFELVGLDPKRMEQYPHQYSGGMKQRAVIALALSCDPKVIIADEPTTALDVIVQDKILKEMKKIQEKLNMAMIYISHDIAVIAEVSDKIAVMYAGKFVELADSITIFKNPMHPYTFLLMNAFPSTIGEKKKLLTIPGEPPNLLNPPKGCRFAPRCPFATEKCKEEEPEYREIEEGHYVACWYPLTKEVRDNAKNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICY0</accession>
    <name>B7ICY0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TPR Domain containing protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1413</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101820.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1413"/>
    <dbReference type="EnsemblBacteria" id="ACJ75857">
        <property type="protein sequence ID" value="ACJ75857"/>
        <property type="gene ID" value="THA_1413"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1413"/>
    <dbReference type="eggNOG" id="COG4783">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1155232_0_0_0"/>
    <dbReference type="OMA" id="DVPWPLY"/>
    <dbReference type="OrthoDB" id="1069317at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002854439" evidence="2">
        <location>
            <begin position="19"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="176"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="230" mass="26615" checksum="509854561AF1F2EB" modified="2009-02-10" version="1">MKKVALIFLILLSSVFFSNSLEENFIKARNFHDIDLIKDVISTAKKEQNNVIYAEALMEYALWGDSEDKEKLYTEALEVIKKEIEKDPNNGRAYYVAGAIVGRLAQYKGIVQSLFMLNDFDNYIEKAIEILDDNFYKGLALIAMGMRFRDVPWPLRSYKKAEKYLLDALEILPNYPNIHLELGKLYEKMGKVEEAKKEYEIVINSNAHPLLNATHEEAKEEARQLLQNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICZ2</accession>
    <name>B7ICZ2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UBA/THIF-type NAD/FAD binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580219.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1425"/>
    <dbReference type="EnsemblBacteria" id="ACJ75869">
        <property type="protein sequence ID" value="ACJ75869"/>
        <property type="gene ID" value="THA_1425"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1425"/>
    <dbReference type="eggNOG" id="COG0476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_10_4_0"/>
    <dbReference type="OMA" id="ACHQATQ"/>
    <dbReference type="OrthoDB" id="1408716at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="PTHR10953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ThiF" evidence="1">
        <location>
            <begin position="15"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="218" mass="24480" checksum="D41CCEAE0F88455F" modified="2009-02-10" version="1">MIDISRHKKLISGLNLKNIKILVAGAGGLGSTVLNNLVRLGFENIIIYDPKMIDPPDLNRQILYNQEDLYQNKAFTALKKLKKINPNCNIKAIDKALSSHFNEKVDIAFDCMDNIEGRLVLEKICKKLQIPMIHGAVQEFSGQVTVIVPGITTSLLDIYEGFDIPKTPQVFPPTVLVTASIQVSEAIKILKDDFKNALINKILFFNLLYNDFEILQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID11</accession>
    <name>B7ID11_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar kinase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1445</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580227.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1445"/>
    <dbReference type="EnsemblBacteria" id="ACJ75888">
        <property type="protein sequence ID" value="ACJ75888"/>
        <property type="gene ID" value="THA_1445"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1445"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_2_2_0"/>
    <dbReference type="OMA" id="AGDCFMA"/>
    <dbReference type="OrthoDB" id="1604782at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="5">
        <location>
            <begin position="7"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="274" mass="30551" checksum="402914E8B0C315EF" modified="2009-02-10" version="1">MFSKEKTDVFCIGKTNLDIFYYIDKIKIEENHVANNFAYFPGGKATNVAINLANLGLKVTLISAIANDIFGRYIKEKLENILIFKPQIVKNENTALTSIVVEGSGANTMFHNLGANQFLSPQAIPDNMNFAFIQTGIPQETLIKSLQSSKTAFVELSETSQFEILKNFSVEYVSLNKDELIKIFKDENIEKNLAKLSYFAKHIVLKMGKEGIIYAGDTIIRKKAKNVKVINTAGAGDAVSAAFIYGTINDWEIEKTLDFSIEFASKIIQSIYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID17</accession>
    <name>B7ID17_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chemotaxis response regulator protein-glutamate methylesterase ofgroup 2 operon, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1451</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580230.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1451"/>
    <dbReference type="EnsemblBacteria" id="ACJ75894">
        <property type="protein sequence ID" value="ACJ75894"/>
        <property type="gene ID" value="THA_1451"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1451"/>
    <dbReference type="eggNOG" id="COG2201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1382319_0_0_0"/>
    <dbReference type="OMA" id="FANHIEI"/>
    <dbReference type="OrthoDB" id="1655418at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008984">
        <property type="term" value="F:protein-glutamate methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035909">
        <property type="entry name" value="CheB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000673">
        <property type="entry name" value="Sig_transdc_resp-reg_Me-estase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01339">
        <property type="entry name" value="CheB_methylest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52738">
        <property type="entry name" value="SSF52738"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50122">
        <property type="entry name" value="CHEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheB-type methylesterase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="183" mass="20613" checksum="6C4E87D2A39B41FE" modified="2009-02-10" version="1">MEKVIIAGSAGSPNNVIKILKINQKLNIPILLCIHFSKNAIENFAHNIKNETGHEVIIVKNPIKLKPAVYLAEGGKDLLLKTSEIVNSGIYKDTKTHPSIEVLLNSIAKLNDKNFHIFILGGLGNDGAKIAKTLEEKGIKFYIEKNPSFEYLTNNFLKNLKYHKILGLEEMKNEIIKINKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID89</accession>
    <name>B7ID89_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">S4 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102055.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1525"/>
    <dbReference type="EnsemblBacteria" id="ACJ75966">
        <property type="protein sequence ID" value="ACJ75966"/>
        <property type="gene ID" value="THA_1525"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1525"/>
    <dbReference type="eggNOG" id="COG1188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101003_4_0_0"/>
    <dbReference type="OMA" id="VANEACD"/>
    <dbReference type="OrthoDB" id="2006597at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025490">
        <property type="entry name" value="RNAbp_HP1423"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038881">
        <property type="entry name" value="RNAbp_HP1423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="79" mass="9405" checksum="7C18373BDF37D6FA" modified="2009-02-10" version="1">MRLDKFLKSTRIIKRRTIAQELAKNKRIFRNQIALKPSSEIKSGDILEIFLKNRYLKVKVISDVEYEILEEKKIEEGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDA8</accession>
    <name>B7IDA8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102213.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1546"/>
    <dbReference type="EnsemblBacteria" id="ACJ75985">
        <property type="protein sequence ID" value="ACJ75985"/>
        <property type="gene ID" value="THA_1546"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1546"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_7_4_0"/>
    <dbReference type="OMA" id="YHVEHLR"/>
    <dbReference type="OrthoDB" id="1772343at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="100" mass="11759" checksum="28C2EFEEAECC6F55" modified="2009-02-10" version="1">MDESIYQLSEFFKILSDQTRLKILIALSKKECSVSELQEFLNTTQSTVSHQLRILRQTNLVKYSKVGRRVYYKLYDEHVKTIINFALEHLEEFRGGSKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDD4</accession>
    <name>B7IDD4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="13">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="13">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 17" type="primary">metG</name>
        <name evidence="17" type="ordered locus">THA_1573</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="13"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102304.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1573"/>
    <dbReference type="EnsemblBacteria" id="ACJ76011">
        <property type="protein sequence ID" value="ACJ76011"/>
        <property type="gene ID" value="THA_1573"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1573"/>
    <dbReference type="eggNOG" id="COG0073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0143">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_9_4_0"/>
    <dbReference type="OMA" id="SDMHGTP"/>
    <dbReference type="OrthoDB" id="761140at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01228">
        <property type="entry name" value="Met_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023457">
        <property type="entry name" value="Met-tRNA_synth_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326">
        <property type="entry name" value="PTHR43326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00399">
        <property type="entry name" value="metG_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13 15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 13 15" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 13 14" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TRNA-binding" evidence="16">
        <location>
            <begin position="537"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="13">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="13">
        <location>
            <begin position="299"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ACJ76011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="638" mass="74076" checksum="BC4F1CDFE0E70A03" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDD7</accession>
    <name>B7IDD7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lactose transport system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1576</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102309.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1576"/>
    <dbReference type="EnsemblBacteria" id="ACJ76014">
        <property type="protein sequence ID" value="ACJ76014"/>
        <property type="gene ID" value="THA_1576"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1576"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_1_0"/>
    <dbReference type="OMA" id="MIGYFET"/>
    <dbReference type="OrthoDB" id="1074175at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="67"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="270" mass="30802" checksum="22FF1E683007F978" modified="2009-02-10" version="1">MKRKISTFFVYLFLSISAFLSIFPFYWMIVGSTNTSVDVIKGKLIFGTHLLENLNKLFKEYDLWRVLLNSVQVSVFVVIGSLIVASMAGYGFEIYKSKVRNKIYGIFIWTLMIPFAALMVPLFRLMNVFNLIDSLWGIILPMIPSVFLIFFFKQNFQQYPKEIIMAARVDGASELRIFFSIVVPSMKATYAAAAIYAFMTSWNAYLWPLVIIQSSEKRTMTLFVSNLASGYKPDFALVMTAVVLATLPMIIVFFALQKYFVQGVLGSVKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDE2</accession>
    <name>GAL1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Galactokinase</fullName>
            <ecNumber evidence="1">2.7.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Galactose kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">galK</name>
        <name type="ordered locus">THA_1581</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-galactose + ATP = ADP + alpha-D-galactose 1-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13553"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28061"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. GalK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.6" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102324.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDE2"/>
    <dbReference type="SMR" id="B7IDE2"/>
    <dbReference type="STRING" id="484019.THA_1581"/>
    <dbReference type="EnsemblBacteria" id="ACJ76019">
        <property type="protein sequence ID" value="ACJ76019"/>
        <property type="gene ID" value="THA_1581"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1581"/>
    <dbReference type="eggNOG" id="COG0153">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017814_2_1_0"/>
    <dbReference type="OMA" id="PVCYNLR"/>
    <dbReference type="OrthoDB" id="1388391at2"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004335">
        <property type="term" value="F:galactokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00246">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000705">
        <property type="entry name" value="Galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022963">
        <property type="entry name" value="Galactokinase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019741">
        <property type="entry name" value="Galactokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019539">
        <property type="entry name" value="GalKase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006206">
        <property type="entry name" value="Mevalonate/galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457:SF6">
        <property type="entry name" value="PTHR10457:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10509">
        <property type="entry name" value="GalKase_gal_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000530">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00473">
        <property type="entry name" value="GALCTOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00131">
        <property type="entry name" value="gal_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00106">
        <property type="entry name" value="GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0299">Galactose metabolism</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000190065" description="Galactokinase">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="14"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="96"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00246"/>
        </source>
    </evidence>
    <sequence length="352" mass="39824" checksum="A59094E2E1F8BBC7" modified="2009-02-10" version="1">MKVKAPGRVNLIGEHTDYNDGFVLPFAIDRYVELEIEESDKFCFYSNNLNEEVKLSSLQKTNSWADYIVGVIKEIEKRGYKIQPVKIKVDSNIPIGAGLSSSAALEVASAYAISEYFGLNLSKIDIVKISREAEANFVGVNCGIMDQFASAFSKKDYAIFLDTMTLDFQFVPLNLKGYEIFVIDSNVKHELSSSEYNLRRQECESALEIIGKDSFRNVTREDLKLLNGGTLLKRVQHILEENERVLKTVKALKENEIERIGEYLYQSHESLRYLYEVSCDETDFIVDFLKEKDGIIGARMVGGGFGGGVIVISKEGSFESIEKLLENEYYSRFGIRPTFYKLNSSDGVTKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDE4</accession>
    <name>B7IDE4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hdig domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1583</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580281.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1583"/>
    <dbReference type="EnsemblBacteria" id="ACJ76021">
        <property type="protein sequence ID" value="ACJ76021"/>
        <property type="gene ID" value="THA_1583"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1583"/>
    <dbReference type="eggNOG" id="COG2206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_92_1_0"/>
    <dbReference type="OMA" id="SDAYWTG"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD-GYP" evidence="2">
        <location>
            <begin position="150"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="172"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="343" mass="39278" checksum="727597891C7F6F08" modified="2009-02-10" version="1">MAIILTIVSVYAVVITALFLKKSLSSSRYNIKSLIYDFNDSLSDSSNDFSSKVLSFISKNFSKIDSGAIFLKNGNSFIELNSFGNKIENNDKIFFYKDYYVKEFFIDESYALRTVFHTIKNLSKEEKEKIEILTGLISSSEIIKNLIKKNGKFQIDLMLSMIKILEYYDRYTQGHSMRVAEISKKIAEKMKLSHKEISDAYWTGLVHDIGKIVIPTGILNKEGKLTNEEFAIIKKHPVYGYEFLSTSETLKDIATYVFHHHERWDGNGYPTGLKGEMIPIISRIIAVADSWDAMISKRAYRNALPKDYALQEIINNSGKQFDPRVVKAFLEVIEEEEDNISIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDE9</accession>
    <name>B7IDE9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ferritin</fullName>
            <ecNumber evidence="1">1.16.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1588</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Iron-storage protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4 Fe(2+) + 6 H2O + O2 = 12 H(+) + 4 iron(III) oxide-hydroxide</text>
            <dbReference type="Rhea" id="RHEA:11972"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:78619"/>
            <dbReference type="EC" id="1.16.3.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ferritin family. Prokaryotic subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.2" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102334.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1588"/>
    <dbReference type="EnsemblBacteria" id="ACJ76026">
        <property type="protein sequence ID" value="ACJ76026"/>
        <property type="gene ID" value="THA_1588"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1588"/>
    <dbReference type="eggNOG" id="COG1528">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065681_1_2_0"/>
    <dbReference type="OMA" id="CEDKGFE"/>
    <dbReference type="OrthoDB" id="1890523at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:cellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01055">
        <property type="entry name" value="Nonheme_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041719">
        <property type="entry name" value="Ferritin_prok"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="PTHR11431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0409">Iron storage</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="2">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="172" mass="20471" checksum="0A1245248CA7AC95" modified="2009-02-10" version="1">MLKEKMTEALNKQINEELYSAYLYLSMSAYFEDMGLKGFANWMMVQAMEERDHAMKIYNYLVNQGARVKLYEIKEPPFEFGSIKNTFEEVLKHEQHITSKINELVDIAEELKDRATFNFLQWYIDEQVEEEENARDILTRLEFVGDDKNALFMLDRELAQRQYVPLNMNKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDI9</accession>
    <name>B7IDI9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1628</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580307.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1628"/>
    <dbReference type="EnsemblBacteria" id="ACJ76066">
        <property type="protein sequence ID" value="ACJ76066"/>
        <property type="gene ID" value="THA_1628"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1628"/>
    <dbReference type="eggNOG" id="ENOG502ZVCH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1585702_0_0_0"/>
    <dbReference type="OMA" id="DYGHYRI"/>
    <dbReference type="OrthoDB" id="1897773at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="169" mass="20057" checksum="A27330B42A2AD1F7" modified="2009-02-10" version="1">MRFIDSFTVKYVRKNKKDYLAKIVKNLSEYHENGLKCEIKSNQNFGRIKLELYETSKEGELILFKGLFFSNIDDFEFSEVRVFSEKLLLNNSLVSDVIVSEISDNVIKGLIYDNDVVYVVLDENKTKEMTNNEISYLVLKYLVENIFEEEFRKDDYELEIETEITDFFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDM7</accession>
    <name>B7IDM7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580336.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1666"/>
    <dbReference type="EnsemblBacteria" id="ACJ76104">
        <property type="protein sequence ID" value="ACJ76104"/>
        <property type="gene ID" value="THA_1666"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1666"/>
    <dbReference type="eggNOG" id="COG2316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090635_1_0_0"/>
    <dbReference type="OMA" id="EMLGWEL"/>
    <dbReference type="OrthoDB" id="1433958at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HDc" evidence="1">
        <location>
            <begin position="16"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="181" mass="20456" checksum="26214E8C575C8B6E" modified="2009-02-10" version="1">MINRQQAWELLNEHVKTKNLIKHCLATEAVMRDLANYFGEDEETWGIAGLLHDLDYEYTKNTPEEHGLKTVELLGDLVSQEIKNAILAHSGKKSRDTLIEKAIYAADPTTGFIVAAALIKPEKKLEAIDVQFLLKRFKEKSFAKGANRDQMASCEKFGLSLEKFYEIALNAMKKISKELGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDN1</accession>
    <name>B7IDN1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nodulation efficiency protein D (NfeD)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1670</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1670"/>
    <dbReference type="EnsemblBacteria" id="ACJ76108">
        <property type="protein sequence ID" value="ACJ76108"/>
        <property type="gene ID" value="THA_1670"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1670"/>
    <dbReference type="eggNOG" id="COG1585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116732_2_1_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002810">
        <property type="entry name" value="NfeD-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01957">
        <property type="entry name" value="NfeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD" evidence="3">
        <location>
            <begin position="20"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="114" mass="12428" checksum="A577D977F3E930B6" modified="2009-02-10" version="1">MASALVAFFVGNTIWELATFIVVSGILVILTRPLANKISGEQTRKINVDEIIGKHALVLEDINNKAGTGIIKVNGDTWRAFSNDDEIVIKKGEYVKILQVEGAHVVVEKIDQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDP5</accession>
    <name>B7IDP5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH dehydrogenase, 30 kDa subunit, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580347.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1685"/>
    <dbReference type="EnsemblBacteria" id="ACJ76122">
        <property type="protein sequence ID" value="ACJ76122"/>
        <property type="gene ID" value="THA_1685"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1685"/>
    <dbReference type="eggNOG" id="COG0852">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042628_6_1_0"/>
    <dbReference type="OMA" id="MEMPWIL"/>
    <dbReference type="OrthoDB" id="1735902at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="SSF143243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex1_30kDa" evidence="1">
        <location>
            <begin position="32"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="176" mass="20800" checksum="F35399F6D2D8469C" modified="2009-02-10" version="1">MTNSMNNALEKLKSLIGEFELIEIDEREVKINLKPESVLQALSLLKGEGYFHLSLLSAVDWIDERKFELVYILYSWNDGGKIIVSTKIDRDKAEFVTVKHMWPVARFYERELHEFFGISFKGNDDMRPLFLENWDDLPPLRKDFDPLEYSKRKFPDREYKKDVIHEAKIIRGDING</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDQ5</accession>
    <name>B7IDQ5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multiple resistance and pH regulation protein F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1695</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CPA3 antiporters (TC 2.A.63) subunit F family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580355.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1695"/>
    <dbReference type="EnsemblBacteria" id="ACJ76132">
        <property type="protein sequence ID" value="ACJ76132"/>
        <property type="gene ID" value="THA_1695"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1695"/>
    <dbReference type="eggNOG" id="COG2212">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125825_2_2_0"/>
    <dbReference type="OMA" id="YERAIYI"/>
    <dbReference type="OrthoDB" id="2083248at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007208">
        <property type="entry name" value="MrpF/PhaF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34702">
        <property type="entry name" value="PTHR34702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04066">
        <property type="entry name" value="MrpF_PhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="85" mass="9323" checksum="286FE9FFA9332209" modified="2009-02-10" version="1">MTIINYIFFGFVGLGVLFSFLRILLGPTSADRVAALDTLNVVLTGTIVFLALVFNNGLYLDIALVYGLLSFTETVVISRYLEGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDS4</accession>
    <name>B7IDS4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1715</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580368.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1715"/>
    <dbReference type="EnsemblBacteria" id="ACJ76151">
        <property type="protein sequence ID" value="ACJ76151"/>
        <property type="gene ID" value="THA_1715"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1715"/>
    <dbReference type="eggNOG" id="COG1452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114532_0_0_0"/>
    <dbReference type="OMA" id="NDEWREV"/>
    <dbReference type="OrthoDB" id="1855303at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="209" mass="23708" checksum="9C106A4115D4526B" modified="2009-02-10" version="1">MKNKFLTFFIILFLATIVISKTIHISSDYIEPSEKTIVYQGNILLKIPEDKLLLTSNDMVISKINNKWIDLNVDGKVHIEFENGVMEGIEMKYNIESQKGTIKNASVTINDSQSSETIFISCETLEFDLKNNTFSGKSEKEKVSITKGSIIAKAFEFWYDRNEGKIILSNNVDLKDEKKKLKLLGESVSINTKDNSMKGKNVKVEIVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDT2</accession>
    <name>B7IDT2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580373.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1723"/>
    <dbReference type="EnsemblBacteria" id="ACJ76159">
        <property type="protein sequence ID" value="ACJ76159"/>
        <property type="gene ID" value="THA_1723"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1723"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_523638_0_0_0"/>
    <dbReference type="OMA" id="LISKTIW"/>
    <dbReference type="OrthoDB" id="365257at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="173"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="440" mass="52431" checksum="34B0626488C92F05" modified="2009-02-10" version="1">MFFENVLKDVPNELSKEVFKAKSALEKNFNDSQVKRNFIEITWELYRYLLENIDENYEVEKRLFLRYGLLEPRYLNSRDFERIKSIPFVDDKENIFYVDEWLLAVKKGLVNESSFMEVIESRKNVAPKNVASFEMAFNEKLEERKFIERQLREIVKGIEDEGKPYRKSILSLIDQAIEKLKKLKLVNKEVEIIFEQLNKLKEESQINEKTEDNFSKKIMEHEVIIQMINKSIGRLGNRFLVLTSIFLNGINAIGSKDLIESKLNEFEKIDYKIFERKIRGTSIKLKPYFIIVPGYGDYGFCWEPFEGLNVISGRGRVVIPMFPKNIDVAILSALGDYRLKAAKELAFGRWMEEGLTGEIYNYLMDNKIRERIDEFFVKRYILWILKESNGVQKLEKEIREIFWRYVEFPEEIKAKLSNISYIYSNLYEKDKRRKKNWENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDX8</accession>
    <name>B7IDX8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">DNA repair protein RecN</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 4">Recombination protein N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1774</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in recombinational repair of damaged DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the RecN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102681.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1774"/>
    <dbReference type="EnsemblBacteria" id="ACJ76205">
        <property type="protein sequence ID" value="ACJ76205"/>
        <property type="gene ID" value="THA_1774"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1774"/>
    <dbReference type="eggNOG" id="COG0497">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018297_3_1_0"/>
    <dbReference type="OMA" id="QVICVTH"/>
    <dbReference type="OrthoDB" id="1074831at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004604">
        <property type="entry name" value="DNA_recomb/repair_RecN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11059">
        <property type="entry name" value="PTHR11059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003128">
        <property type="entry name" value="RecN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="142"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="291"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00033408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR003128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="506" mass="58687" checksum="EC869C1E066582B2" modified="2009-02-10" version="1">MKSVEVVSVHLHDYLYFKDVDIYFDKGLNVITGETGAGKSLMLDIFGILLGVTPGRVDNYSVEVVIDIPDDILEYEIYKGENVFSVKRNNSRTTFKINGRVYPKNIVSSILSNYITLHRQNSHMKFLEQNFLISILDEISNNKNLLNEYRKYYELYKELNELIKSESLNKLYEKLEYLESVIKEIEEVNPTVEEEELLNEQYNVATKLQETVEKYNEILNESEVISEKLWNIKKIINDKYEESVNAALDIVETLKLDIQKELSEIEGYDVKEIEEKIWQYNQLKRKYGPTLEDVLLNYENYMKELNEIKKKIELLEDSQKKIEDCLKNMKELSEKISLNRKKAAEKVSEAFLKHCNDLNLNIEIQFNFSKIDYNYNGFDKVELLGRTVKNQPLKPLKVIASGGELSRLMLALELSVVSDGILIFDEVDAGISGETGNKLAEKLKEVSERYQVIVVTHLPQVAVRADKHFVVLKDQKSGLIKELDEEERSIEIKRMVGSDDVLKFID</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDY7</accession>
    <name>RBFA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome-binding factor A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rbfA</name>
        <name type="ordered locus">THA_1783</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Binds 30S ribosomal subunits, but not 50S ribosomal subunits or 70S ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RbfA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580408.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDY7"/>
    <dbReference type="SMR" id="B7IDY7"/>
    <dbReference type="STRING" id="484019.THA_1783"/>
    <dbReference type="EnsemblBacteria" id="ACJ76214">
        <property type="protein sequence ID" value="ACJ76214"/>
        <property type="gene ID" value="THA_1783"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1783"/>
    <dbReference type="eggNOG" id="COG0858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089475_6_5_0"/>
    <dbReference type="OMA" id="KGYFRTY"/>
    <dbReference type="OrthoDB" id="1971380at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00003">
        <property type="entry name" value="RbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000238">
        <property type="entry name" value="RbfA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023799">
        <property type="entry name" value="RbfA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020053">
        <property type="entry name" value="Ribosome-bd_factorA_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33515">
        <property type="entry name" value="PTHR33515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02033">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89919">
        <property type="entry name" value="SSF89919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00082">
        <property type="entry name" value="rbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01319">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_1000193273" description="Ribosome-binding factor A">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00003"/>
        </source>
    </evidence>
    <sequence length="126" mass="14984" checksum="FB383983D3AF6A90" modified="2009-02-10" version="1">MRPEYRNKKIEAYIRELIAQAIQKIKEPEFEYLKDYIVISRVLISKDRRFADVFVSVIGNSEQRKKAVELLEKYKGYFRTFVAKNVRLYTAPELRFKEDKGIEESVRINKLLDEIMSKEDENGSDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDZ2</accession>
    <name>B7IDZ2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Lon protease</fullName>
            <ecNumber evidence="5 6">3.4.21.53</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-dependent protease La</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">lon</name>
        <name evidence="13" type="ordered locus">THA_1788</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6 9">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 6">Homohexamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="5">By heat shock.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6 9 10">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="5 6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1788"/>
    <dbReference type="MEROPS" id="S16.001"/>
    <dbReference type="EnsemblBacteria" id="ACJ76219">
        <property type="protein sequence ID" value="ACJ76219"/>
        <property type="gene ID" value="THA_1788"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1788"/>
    <dbReference type="eggNOG" id="COG0466">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004109_4_3_0"/>
    <dbReference type="OMA" id="KKMNPVM"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01973">
        <property type="entry name" value="lon_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027543">
        <property type="entry name" value="Lon_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001174">
        <property type="entry name" value="Lon_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="12">
        <location>
            <begin position="12"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="11">
        <location>
            <begin position="591"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 7 9">
        <location>
            <position position="676"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 7 9">
        <location>
            <position position="719"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <begin position="356"/>
            <end position="363"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ76219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="775" mass="88605" checksum="42A9FCFADF3D2476" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE13</accession>
    <name>B7IE13_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transporter, auxin efflux carrier (AEC) family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1809</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the auxin efflux carrier (TC 2.A.69) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580433.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1809"/>
    <dbReference type="EnsemblBacteria" id="ACJ76240">
        <property type="protein sequence ID" value="ACJ76240"/>
        <property type="gene ID" value="THA_1809"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1809"/>
    <dbReference type="eggNOG" id="COG0679">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056175_4_0_0"/>
    <dbReference type="OMA" id="TTFFWTL"/>
    <dbReference type="OrthoDB" id="2033473at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="293" mass="33293" checksum="48D6392883C3DA59" modified="2009-02-10" version="1">MINAFLTILPPFLIIIVGYIFGRIFPYDIKIISKISLWIMASVLSFTFINDYPPKLSYLKSYGLGILFLFILFYILSFAFKKDRFLILTNSIYINTGYLGYPILYSIWGEQAMSYGVIYSVINMLVASIVLPSFIGEKINFKNILKLPYLYVITFAYLLSIFGISYKQLPVPFIETINMLKNSAIPFLLIFTGLSLSKIKIKSVNFYTVIFSTIIRLVLYPAFALIFVFFSKMDNEFARVFVLESAMPTAINSVILIDAITGDASKISLTVAITTLLSAFTIPVWIIVLERIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE20</accession>
    <name>B7IE20_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA protecting protein DprA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dprA</name>
        <name evidence="2" type="ordered locus">THA_1816</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DprA/Smf family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580439.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1816"/>
    <dbReference type="EnsemblBacteria" id="ACJ76247">
        <property type="protein sequence ID" value="ACJ76247"/>
        <property type="gene ID" value="THA_1816"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1816"/>
    <dbReference type="eggNOG" id="COG0758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029601_0_2_0"/>
    <dbReference type="OMA" id="VYCYPGL"/>
    <dbReference type="OrthoDB" id="1881437at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009294">
        <property type="term" value="P:DNA-mediated transformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003488">
        <property type="entry name" value="DprA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43022">
        <property type="entry name" value="PTHR43022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02481">
        <property type="entry name" value="DNA_processg_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00732">
        <property type="entry name" value="dprA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="266" mass="30224" checksum="FEFE4E3BCD1A54E1" modified="2009-02-10" version="1">MTKVEIASLSSLGYSINEIKNGYIKSKDIQRKKFIESKLTRWLEFSGNGILTFFDDEYHEYLKNIWKPPVVLFYKGNIHLLKKNCFSVVGTRSMTAYGKSITEKFVKKLSDFFVIVSGMAIGIDTEAHKNSKQTIAVLGCGVDICYPKQNKYIYDKISKEGCLISEYLPWENPKKHYFPLRNRIIAGISEGVLVVECKKKSGTMITANYAIDFGKDLFTVPGNIFSLNSEGPNFLIKNGAYPVTDPDEILDFYIMRGVINESNRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE22</accession>
    <name>B7IE22_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alpha-glucosidase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1818</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580441.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1818"/>
    <dbReference type="CAZy" id="GH31">
        <property type="family name" value="Glycoside Hydrolase Family 31"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76249">
        <property type="protein sequence ID" value="ACJ76249"/>
        <property type="gene ID" value="THA_1818"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1818"/>
    <dbReference type="eggNOG" id="COG1501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000631_7_2_0"/>
    <dbReference type="OMA" id="QGVDCFK"/>
    <dbReference type="OrthoDB" id="469334at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033403">
        <property type="entry name" value="DUF5110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17137">
        <property type="entry name" value="DUF5110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gal_mutarotas_2" evidence="3">
        <location>
            <begin position="61"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5110" evidence="4">
        <location>
            <begin position="634"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="702" mass="82594" checksum="4EA75C7FEAF00562" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE26</accession>
    <name>HSLU_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent protease ATPase subunit HslU</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Unfoldase HslU</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hslU</name>
        <name type="ordered locus">THA_1822</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpX chaperone family. HslU subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580445.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IE26"/>
    <dbReference type="SMR" id="B7IE26"/>
    <dbReference type="STRING" id="484019.THA_1822"/>
    <dbReference type="EnsemblBacteria" id="ACJ76253">
        <property type="protein sequence ID" value="ACJ76253"/>
        <property type="gene ID" value="THA_1822"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1822"/>
    <dbReference type="eggNOG" id="COG1220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033123_0_0_0"/>
    <dbReference type="OMA" id="KYGMIKT"/>
    <dbReference type="OrthoDB" id="718259at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009376">
        <property type="term" value="C:HslUV protease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036402">
        <property type="term" value="F:proteasome-activating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00249">
        <property type="entry name" value="HslU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004491">
        <property type="entry name" value="HslU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48102:SF3">
        <property type="entry name" value="PTHR48102:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00390">
        <property type="entry name" value="hslU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_1000119114" description="ATP-dependent protease ATPase subunit HslU">
        <location>
            <begin position="1"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="63"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00249"/>
        </source>
    </evidence>
    <sequence length="459" mass="52470" checksum="DBF7B935BFEC3BED" modified="2009-02-10" version="1">MSDFDKLTPKQIVEELDKYIVGQSKAKKAVAIAIRNRIRRQKLSEEWKKEITPKNILMIGPTGVGKTEIARRLAQLSGSPFLKIEATRFTEVGYVGKNVDSMIRELVEIAVNMVKQQKMKEVEEKAKLNVEERILDALVPMKKKTQIPFANIFGMQMEKPQQTDDYSENLRKREELRRRLRSGELDNEEIEIEIETSNSPIGFIGLPEMEDIGMDLSNVIGNIFPKQKKRRKMKISEAKKVLLPIEEEKLIDMDETIQTALELAQNRGIIFIDEMDKIAAKTGSSGQDVSRQGVQRDLLPIVEGTTITTKYGPVKTDYILFIAAGAFHVSKPSDLIPELQGRFPIRVELEPLKEEDFVRILVEPENALTKQYQALLYTENVQLEFTDDGIKELARVSYKLNQKLENIGARRLYTVLEKVLEDVLFEAPEIEEKIIVDADYVTKKLKGIIEDEDLTSYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE57</accession>
    <name>B7IE57_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10 11">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="7 10 11">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpe</name>
        <name evidence="15" type="ordered locus">THA_1858</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10 13">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 10 11">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="7 10 11"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580461.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1858"/>
    <dbReference type="EnsemblBacteria" id="ACJ76284">
        <property type="protein sequence ID" value="ACJ76284"/>
        <property type="gene ID" value="THA_1858"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1858"/>
    <dbReference type="eggNOG" id="COG0036">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054856_2_1_0"/>
    <dbReference type="OMA" id="WLQVDGG"/>
    <dbReference type="OrthoDB" id="1697639at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="PTHR11749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="13" id="KW-0170">Cobalt</keyword>
    <keyword evidence="9 10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="140"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="173"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10 12">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10 12">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 14">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13 14">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ACJ76284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="213" mass="24016" checksum="D4AAD618A31272EC" modified="2009-02-10" version="1">MVKLSGSILAANLSNLESEIKRVEKFIDEIHFDTMDGHFVPNLTFGYPLIEAVKNCTNLPIDVHLMVTNPEFHVEKLKEFDIQRITVHLETTYHIHRLISRIKEYGFEAFVALNPGTPLYLIEEIIPFLDGVLVMSVNPGFSGQKFIAKSIEKIKTLKNWKEKYGLNFSIAVDGGINETNADVVSSAGADILVMGYGIFKNNNLEQFSKLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE67</accession>
    <name>B7IE67_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mannose-6-phosphate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1868</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the mannose-6-phosphate isomerase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102859.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1868"/>
    <dbReference type="EnsemblBacteria" id="ACJ76294">
        <property type="protein sequence ID" value="ACJ76294"/>
        <property type="gene ID" value="THA_1868"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1868"/>
    <dbReference type="eggNOG" id="COG1482">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020529_0_0_0"/>
    <dbReference type="OMA" id="DTFFINT"/>
    <dbReference type="OrthoDB" id="645703at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001250">
        <property type="entry name" value="Man6P_Isoase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01238">
        <property type="entry name" value="PMI_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="262" mass="29969" checksum="B69A527CCDED5519" modified="2009-02-10" version="1">MIKIEPKFREQVWGDFNLNKTFGIKGNPIGEVWLLSGHPLYTTNINGKTVNEASNELCGKEFKRFPLLIKLISTNAWLSLQVHPDDEYAQRVEGEPWGKSEAWYFLKDGEIAICENPSLIPQALKSGNWEKVIRKVKVKKGTFVYIPAGVIHALGPNSTIIEVQQSSDLTYRIYDWGRQRELHIEKALQVAKKTKYENLLMEKISCEYFNMEELFEGYVDGFAVAVPKKIEKNFGAFLIPKGSKEFVRDSIIITLGEFFLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE69</accession>
    <name>B7IE69_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 9">Acyl carrier protein</fullName>
            <shortName evidence="7">ACP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">acpP</name>
        <name evidence="11" type="synonym">acpP2</name>
        <name evidence="11" type="ordered locus">THA_1870</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 9">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by AcpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by acpS.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580466.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1870"/>
    <dbReference type="EnsemblBacteria" id="ACJ76296">
        <property type="protein sequence ID" value="ACJ76296"/>
        <property type="gene ID" value="THA_1870"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1870"/>
    <dbReference type="eggNOG" id="COG0236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108696_5_4_0"/>
    <dbReference type="OMA" id="MFFEDEF"/>
    <dbReference type="OrthoDB" id="1943389at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="Acyl_carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="PTHR20863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1 7 8" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3 7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="10">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="7 8">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ76296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="84" mass="9504" checksum="B645C2B19DA78A82" modified="2009-02-10" version="1">MERAELFRQVSKILAEKLNIPIEDIDESSNIIDDLGADSLDVVDLVMILEDEYGIRIEDDELENISTVEDVINIIESKLKVQGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE82</accession>
    <name>B7IE82_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Septum site-determining protein MinD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cell division inhibitor MinD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">minD</name>
        <name evidence="8" type="ordered locus">THA_1883</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">ATPase required for the correct placement of the division site. Cell division inhibitors MinC and MinD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParA family. MinD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102882.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1883"/>
    <dbReference type="EnsemblBacteria" id="ACJ76309">
        <property type="protein sequence ID" value="ACJ76309"/>
        <property type="gene ID" value="THA_1883"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1883"/>
    <dbReference type="eggNOG" id="COG2894">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_0_1_0"/>
    <dbReference type="OMA" id="CESAKAY"/>
    <dbReference type="OrthoDB" id="1631847at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010223">
        <property type="entry name" value="MinD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01968">
        <property type="entry name" value="minD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="7">
        <location>
            <begin position="6"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="267" mass="29383" checksum="BBB6E81479D4E77C" modified="2009-02-10" version="1">MAKVYVVTSGKGGVGKTTISANLGCALAKDGNKVCVIDADVGLKNLDVVLGLENRIIYTLIDVIRGNVSAKEALVRHKNLKNLYLIAASQIATKEMVSPEDMINLVKELDEDFDYIIIDSPAGIERGFRNAIAPSEYAIVVTTPELPAISDADRVIGLLENNGFNEENIMLILNKYKPQMVKKGDMLSEADVEKALAIKLIGVLPDSNEVIISTNKGIPIVLEKDEGISKNFENIVKRLKGEEISLAEDIQNNENFFRKFLSFFNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE83</accession>
    <name>B7IE83_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1884</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102883.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1884"/>
    <dbReference type="EnsemblBacteria" id="ACJ76310">
        <property type="protein sequence ID" value="ACJ76310"/>
        <property type="gene ID" value="THA_1884"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1884"/>
    <dbReference type="eggNOG" id="COG0851">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187549_0_0_0"/>
    <dbReference type="OMA" id="MWIIDIF"/>
    <dbReference type="OrthoDB" id="1879876at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032955">
        <property type="term" value="P:regulation of division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036707">
        <property type="entry name" value="MinE_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="88" mass="10500" checksum="FADFA42D23A71F15" modified="2009-02-10" version="1">MWLLDVFKRSEKRKPRDEALERLESIVSRRKEITHKIPVEVFSKNSEEIKKEVVQIISRKFNVPVERVKVDCQEEDGYVVIVTNINFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE85</accession>
    <name>B7IE85_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1886</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580468.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1886"/>
    <dbReference type="EnsemblBacteria" id="ACJ76312">
        <property type="protein sequence ID" value="ACJ76312"/>
        <property type="gene ID" value="THA_1886"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1886"/>
    <dbReference type="HOGENOM" id="CLU_199885_0_0_0"/>
    <dbReference type="OMA" id="SFIRYRV"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="75" mass="9243" checksum="84425A4BDF9B1F12" modified="2009-02-10" version="1">MGRKKKKNLFRYIYILFVLLLVFFLVVILASSIIRYKVNLNELNQVKKQYEELKEKYEKNLIIYEKLKMLREGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEC5</accession>
    <name>B7IEC5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Succinyl-diaminopimelate desuccinylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1929</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102978.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1929"/>
    <dbReference type="EnsemblBacteria" id="ACJ76352">
        <property type="protein sequence ID" value="ACJ76352"/>
        <property type="gene ID" value="THA_1929"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1929"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031786_2_0_0"/>
    <dbReference type="OMA" id="GYAGHIE"/>
    <dbReference type="OrthoDB" id="906744at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010964">
        <property type="entry name" value="M20A_pepV-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01887">
        <property type="entry name" value="dipeptidaselike"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="2">
        <location>
            <begin position="260"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="464" mass="51776" checksum="BD9FE6C1FF8D6562" modified="2009-02-10" version="1">MEQIVEDAIDKLVDKYFDEVLENLEKIIEIKSVMGPASQDAPFGVGPAKALSEALKISKKLGFETKNIDFYAGHVTYGNSGKLYGILGHLDVVPEGDLEKWETDPYKLVIRDGKMFGRGVSDDKGPTIGALYALKIASELVKTPKNTVRLIFGTNEENGSKCLKYYFKNEPYPDAAVTPDGTFPLVFAEKGNTTYKISTYLNNDYNTKLIKMKAGTAVNVVPEECNVVIETDKVNEVAYLVENFNTKCKLKYEVNGNRISITTIGKSAHASTPQLGLNSIACMLNLLSNIDFGKDNFVIRVLNEKLGLDINGIRLGIYSKDIASGELTCNLGTINIENGDLEVKINIRYPIFMNIEMITNQIKEAFKEFDLLQISHSKPLYVSKDSELVKMLLKVYRDVTKDEKEPIALGGGTYAKSVPYGVAFGAVFPGEDTGMHQPNEHWSLESYKKFIRIYARLIYKWLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEC7</accession>
    <name>B7IEC7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bacterial extracellular solute-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580489.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1931"/>
    <dbReference type="EnsemblBacteria" id="ACJ76354">
        <property type="protein sequence ID" value="ACJ76354"/>
        <property type="gene ID" value="THA_1931"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1931"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_309660_0_0_0"/>
    <dbReference type="OMA" id="YDFYNRF"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="964" mass="112058" checksum="28A0E5B5A505E89F" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IED1</accession>
    <name>B7IED1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1935</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580490.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1935"/>
    <dbReference type="EnsemblBacteria" id="ACJ76358">
        <property type="protein sequence ID" value="ACJ76358"/>
        <property type="gene ID" value="THA_1935"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1935"/>
    <dbReference type="eggNOG" id="COG3420">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014011_0_0_0"/>
    <dbReference type="OMA" id="TWTARNE"/>
    <dbReference type="OrthoDB" id="54422at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043751">
        <property type="entry name" value="DUF5696"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18952">
        <property type="entry name" value="DUF5696"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="746" mass="86152" checksum="A4ABE43A119F9DF5" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IED8</accession>
    <name>B7IED8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1943</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103016.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1943"/>
    <dbReference type="EnsemblBacteria" id="ACJ76365">
        <property type="protein sequence ID" value="ACJ76365"/>
        <property type="gene ID" value="THA_1943"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1943"/>
    <dbReference type="eggNOG" id="COG4902">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051317_1_2_0"/>
    <dbReference type="OMA" id="YITSAYF"/>
    <dbReference type="OrthoDB" id="1938827at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01048">
        <property type="entry name" value="Ferritin_like_AB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019243">
        <property type="entry name" value="DUF2202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09968">
        <property type="entry name" value="DUF2202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002854475" description="DUF2202 domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2202" evidence="2">
        <location>
            <begin position="40"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="203" mass="23554" checksum="6FFF515A6E7B822E" modified="2009-02-10" version="1">MKKVIAFAVLNFLSIVLFAGNLTVDKQVNSLPYETLSASEVEGIMQMREEEKLARDVYLELYKTWDLPVFYNIAQSEQTHMDAVKTLIEKYNLKDPITDEDFENIGVFKNEELQNLFNELLSLGEKSLSDALKVGATIEDLDIFDLEELLKSTDNEDIKFIYNNLMKGSENHMRSFIKNLDKLGKTYTPQFISLERFREILKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEE4</accession>
    <name>B7IEE4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1949</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76371">
        <property type="protein sequence ID" value="ACJ76371"/>
        <property type="gene ID" value="THA_1949"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1949"/>
    <dbReference type="HOGENOM" id="CLU_2385153_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="94" mass="10840" checksum="B09B9A233FF1AC6E" modified="2009-02-10" version="1">MIELIVFQFLQGKVQTRFTTLTPKPNARFQFLQGKVQTQEPPSISIDAKPKFQFLQGKVQTFLSQHNTAVVSDLFQFLQGKVQTILDIFFSIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEF1</accession>
    <name>B7IEF1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CRISPR-associated exonuclease Cas4</fullName>
            <ecNumber evidence="6">3.1.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1956</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Mg(2+) or Mn(2+) required for ssDNA cleavage activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CRISPR-associated exonuclease Cas4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.12.1" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580504.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1956"/>
    <dbReference type="EnsemblBacteria" id="ACJ76378">
        <property type="protein sequence ID" value="ACJ76378"/>
        <property type="gene ID" value="THA_1956"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1956"/>
    <dbReference type="eggNOG" id="COG1468">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133784_0_0_0"/>
    <dbReference type="OMA" id="CKRELWF"/>
    <dbReference type="OrthoDB" id="1594787at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013343">
        <property type="entry name" value="CRISPR-assoc_prot_Cas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00372">
        <property type="entry name" value="cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 6" id="KW-0464">Manganese</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas_Cas4" evidence="7">
        <location>
            <begin position="3"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="158" mass="18518" checksum="5BFA561248FA2919" modified="2009-02-10" version="1">MITGSLIQAYIVCKRQAWLLSRQLIGEQDSDFLSIGRLISQESYSRDKKEITIDGGKVDIVRLENGKFKLVEVKKSSKMIKSAKFQLLYYMWKMNVDYGEIRIPKEKKVIEVRMTNEVKEKLLGLISEIEDIVNKDKVPKAERKKYCKTCSFEYFCWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEK6</accession>
    <name>B7IEK6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_12</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_12"/>
    <dbReference type="EnsemblBacteria" id="ACJ74520">
        <property type="protein sequence ID" value="ACJ74520"/>
        <property type="gene ID" value="THA_12"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_12"/>
    <dbReference type="HOGENOM" id="CLU_3206348_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="45" mass="5540" checksum="7A5316D72EB94D8E" modified="2009-02-10" version="1">MYKKKKHMEKLRDKLENEIPNTERNEKLLIKVWDIADILVNKFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEM4</accession>
    <name>B7IEM4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_30</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579304.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_30"/>
    <dbReference type="EnsemblBacteria" id="ACJ74538">
        <property type="protein sequence ID" value="ACJ74538"/>
        <property type="gene ID" value="THA_30"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_30"/>
    <dbReference type="eggNOG" id="COG4608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_0"/>
    <dbReference type="OMA" id="AFGPKVH"/>
    <dbReference type="OrthoDB" id="1303270at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="8"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="51"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="380" mass="43408" checksum="444C910CBEEBE9BD" modified="2009-02-10" version="1">MSEKKVLVKVDNLVKYFPIRAGVFKRIVAWVRAVDDISFEIYEGETVGLVGESGCGKTTAGMTLLRLYEPTSGRIIVDGKDTTYYFMPRYRAKKYLKKMYIEKFKKEDPSKFTGIDKEYYELYEELGESGFYSKLLDNITEKRRDFRRTMQIVFQDPYSSLNPRIRVKTIVSEGPVLHGLIKKSEVVDKVKEVLEEVGIHGEHMYRFPHQFSGGQRQRIGIARALLMNPKLIVADEAVAALDVSIRSQVINLMLELQKKHNLTYLFISHDLSVIKYISDRVVVMYLGKIVENASKKELFDNPLHPYTKALMSAIPVPNPEYKKKRIILTGDVPSPVNPPSGCRFHPRCPVAKEICAKEEPQLKEVSPGHFVACHFPGSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IET6</accession>
    <name>B7IET6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methyl-accepting chemotaxis protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_92</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579343.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_92"/>
    <dbReference type="EnsemblBacteria" id="ACJ74600">
        <property type="protein sequence ID" value="ACJ74600"/>
        <property type="gene ID" value="THA_92"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_92"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_19_0"/>
    <dbReference type="OrthoDB" id="477199at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="6">
        <location>
            <begin position="307"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="386"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="376"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="604"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="665" mass="72832" checksum="C07C6A22DA7FFB71" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEV1</accession>
    <name>B7IEV1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579351.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_107"/>
    <dbReference type="EnsemblBacteria" id="ACJ74615">
        <property type="protein sequence ID" value="ACJ74615"/>
        <property type="gene ID" value="THA_107"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_107"/>
    <dbReference type="eggNOG" id="ENOG5032WN1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173848_0_0_0"/>
    <dbReference type="OMA" id="LVVYIKC"/>
    <dbReference type="OrthoDB" id="1907777at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="106" mass="12637" checksum="84E9525D0405E9E7" modified="2009-02-10" version="1">MFGFFKKKKNIEKNPFYNEKDSLVIYIKCDKCGEVFRSHLRKGYDFFNEYDNPNIRYKIEKLYVGSKCPNKIEVRAQFTGSYKPVSFDISGGKFISKEEYEKEVEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEV3</accession>
    <name>RS20_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsT</name>
        <name type="ordered locus">THA_109</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103501.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IEV3"/>
    <dbReference type="SMR" id="B7IEV3"/>
    <dbReference type="STRING" id="484019.THA_109"/>
    <dbReference type="EnsemblBacteria" id="ACJ74617">
        <property type="protein sequence ID" value="ACJ74617"/>
        <property type="gene ID" value="THA_109"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_109"/>
    <dbReference type="eggNOG" id="COG0268">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160655_5_0_0"/>
    <dbReference type="OMA" id="GVIHKNA"/>
    <dbReference type="OrthoDB" id="2005443at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00500">
        <property type="entry name" value="Ribosomal_S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002583">
        <property type="entry name" value="Ribosomal_S20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036510">
        <property type="entry name" value="Ribosomal_S20_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33398">
        <property type="entry name" value="PTHR33398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01649">
        <property type="entry name" value="Ribosomal_S20p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46992">
        <property type="entry name" value="SSF46992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00029">
        <property type="entry name" value="S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000126528" description="30S ribosomal protein S20">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00500"/>
        </source>
    </evidence>
    <sequence length="92" mass="10542" checksum="47E1D8DEF435AB4D" modified="2009-02-10" version="1">MPNIKSAKKRVKVSERNRLINKAYKTRMKNSIKKVLLALQEGKSREEVEQLYKVAQSAIDKAAKIGAIHKNQASRRKSRLIAKINKHFASKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEY4</accession>
    <name>B7IEY4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103577.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_140"/>
    <dbReference type="EnsemblBacteria" id="ACJ74648">
        <property type="protein sequence ID" value="ACJ74648"/>
        <property type="gene ID" value="THA_140"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_140"/>
    <dbReference type="HOGENOM" id="CLU_2738701_0_0_0"/>
    <dbReference type="OMA" id="FSPINIY"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="71" mass="8280" checksum="30CD886702766B43" modified="2009-02-10" version="1">MNYIEILSNIFSPINIYESDDFVTIVIENDENLEEKIKKTPKNMLPEKTLRIITKEELENNAIKDLGVKLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF13</accession>
    <name>DAPA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <shortName evidence="1">HTPA synthase</shortName>
            <ecNumber evidence="1">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapA</name>
        <name type="ordered locus">THA_172</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579388.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IF13"/>
    <dbReference type="SMR" id="B7IF13"/>
    <dbReference type="STRING" id="484019.THA_172"/>
    <dbReference type="EnsemblBacteria" id="ACJ74677">
        <property type="protein sequence ID" value="ACJ74677"/>
        <property type="gene ID" value="THA_172"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_172"/>
    <dbReference type="eggNOG" id="COG0329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_7_0_0"/>
    <dbReference type="OMA" id="GMDACVP"/>
    <dbReference type="OrthoDB" id="1438588at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00950">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00418">
        <property type="entry name" value="DapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005263">
        <property type="entry name" value="DapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020624">
        <property type="entry name" value="Schiff_base-form_aldolases_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00674">
        <property type="entry name" value="dapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00665">
        <property type="entry name" value="DHDPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_1000124072" description="4-hydroxy-tetrahydrodipicolinate synthase">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="1">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="1">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="293" mass="32441" checksum="C5374065E2284974" modified="2009-02-10" version="1">MFEGIGTAIVTPFKEGKLDIESYEKLLNFQINNQINAIIVLGTTGEAPNISLDEREILIKKAKEVSGNKAKVIVGAGSNGINHTIELIKNAEKNGADGLLIVTPYYNKPTQNGLYEYYKYISEHTDLEIIVYNVPSRTGINILPETVYKIASDCKNVKALKEANSSFEQINKDLLLLKELKDFKIFSGNDDTAFQLLASGGDGVISVASNIIPLQMVQMFNFIKNGEIEKARNIHFSYYKLFKNLFIETNPIPVKAALSIMGFINNELRLPLVPASESTMKIITETLKGCGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF25</accession>
    <name>B7IF25_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alcohol dehydrogenase, iron-containing</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579398.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_184"/>
    <dbReference type="EnsemblBacteria" id="ACJ74689">
        <property type="protein sequence ID" value="ACJ74689"/>
        <property type="gene ID" value="THA_184"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_184"/>
    <dbReference type="eggNOG" id="COG1454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007207_0_0_0"/>
    <dbReference type="OMA" id="HAMSHQV"/>
    <dbReference type="OrthoDB" id="1456634at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="PTHR11496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-ADH" evidence="2">
        <location>
            <begin position="6"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="359" mass="40071" checksum="58E929F1AE9A57AC" modified="2009-02-10" version="1">MRFFLPTKVFFEDNYNESVDEIKKLGESFLIITGKSSKKNGSLEELTNIFEKLNKKYEIFDETLENPPKEMIEEIIRKFGKNWDVIVGLGGGSPMDAAKAIAVLCKNDISIEDLYVQSKYKTASKIICIPTTSGTGSEVTQYSVLTINNVKKGFKHEIVFPTLSILEPKYTLTMSKELTISTGLDALSHAVESALSLKSNSFSDLYAFKAIEIISQDLPKLVNNLSDYSLRRNLMLASMYAGVAISVTGTTIAHSLGYSLTTDKNIKHGLATAVFLPFEIENSGSKKAEEIKAIMGNILNFLKKLEINLNFEVSDDEIEKWAERVKNSSHVKVTPGNYTKEKIIKAYKWFFEESGLFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF64</accession>
    <name>B7IF64_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">D-aminopeptidase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579426.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_223"/>
    <dbReference type="EnsemblBacteria" id="ACJ74728">
        <property type="protein sequence ID" value="ACJ74728"/>
        <property type="gene ID" value="THA_223"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_223"/>
    <dbReference type="eggNOG" id="COG2362">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086038_0_0_0"/>
    <dbReference type="OMA" id="ATGVTWP"/>
    <dbReference type="OrthoDB" id="1439650at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027476">
        <property type="entry name" value="DppA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007035">
        <property type="entry name" value="Peptidase_M55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036177">
        <property type="entry name" value="Peptidase_M55_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04951">
        <property type="entry name" value="Peptidase_M55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015853">
        <property type="entry name" value="Pep_DppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63992">
        <property type="entry name" value="SSF63992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR015853-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR015853-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="271" mass="31016" checksum="D84D349DFBE5EE62" modified="2009-02-10" version="1">MKIYVSIDFEGLGGVTQWSDVEYGVKFKQNYLMEQLKAFLRATDGNYVLLVDSHAMGDNILWEITKEFENVELISGGLRKNYMMSGLDESFDRVVFFGYHAGVGTRYAVMDHSYSSSSIYNIWINGQKMNEALINAAYAGLYNVPVAMIVGDDKLKGQVFDDVFFVETKESLGRFSAKHKSMKKVLKEIEETTRRMLQVPREKFKVYKFDTPVELVVEFTDTARADLVEMLPLVERVNGRSVKLVHEDYQVIFDAILSMAFMCSVVKYIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF67</accession>
    <name>B7IF67_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579427.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_226"/>
    <dbReference type="EnsemblBacteria" id="ACJ74731">
        <property type="protein sequence ID" value="ACJ74731"/>
        <property type="gene ID" value="THA_226"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_226"/>
    <dbReference type="eggNOG" id="ENOG5032YVC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028669_0_0_0"/>
    <dbReference type="OMA" id="RWILAYY"/>
    <dbReference type="OrthoDB" id="793700at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="526"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="550"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="577"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="606" mass="70784" checksum="0FAA0321CF749096" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF71</accession>
    <name>B7IF71_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sugar ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_230</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103772.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_230"/>
    <dbReference type="EnsemblBacteria" id="ACJ74735">
        <property type="protein sequence ID" value="ACJ74735"/>
        <property type="gene ID" value="THA_230"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_230"/>
    <dbReference type="eggNOG" id="COG1079">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040769_1_0_0"/>
    <dbReference type="OMA" id="MIFGNWM"/>
    <dbReference type="OrthoDB" id="1215743at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="319" mass="34094" checksum="DE42ECC0888B306F" modified="2009-02-10" version="1">MRVLEAIFLVFINPLFYKITLTAATPLIFASLGGVYSEITGVVNIALEGIMLMSAFTSVVFTHLSGNVWIGVLMAVISGLAFSWIHAWGSIKWAGNQVVLGTALILIAQGVTGFLMEPIFGKPGQTDFVGKVEELKIPGLSNVPFFGKIFGELSPFVYMAFAAVAFSWWLIYKTKLGLRMRAVGENPEAADTLGINVFGIRYFGVLMSGVWAGLAGAYLSIGEVGHFRELMTGGRGFIALAAMILGKYNPVGAMLASLLFGASSAFANQMQSSSLIHVSATVKPLFDMIPFILTIIVVAGFVGKSRAPKADGVPYEKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF75</accession>
    <name>B7IF75_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DegV family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_236"/>
    <dbReference type="EnsemblBacteria" id="ACJ74739">
        <property type="protein sequence ID" value="ACJ74739"/>
        <property type="gene ID" value="THA_236"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_236"/>
    <dbReference type="eggNOG" id="COG1307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048251_4_1_0"/>
    <dbReference type="OMA" id="MALGFCA"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003797">
        <property type="entry name" value="DegV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043168">
        <property type="entry name" value="DegV_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02645">
        <property type="entry name" value="DegV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00762">
        <property type="entry name" value="DegV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51482">
        <property type="entry name" value="DEGV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="246" mass="27985" checksum="902EBC21213A65DD" modified="2009-02-10" version="1">MKTKIIVDSTSDIPVSWIEKYDIDVVPLYVNWPDGSSEPDNVRDEESLKEFYERLSKAEELPKTSQPSILDFQNAYKRAIEDGYDSVLVLTISTKMSGTFNSARLAAESFDIPIEIVDTKMASSIISNMAKYARELLNSGMSLEEVAKKVREERENKRFHAIFFVSDFDFLAKGGRVSKFTGFVGNLLKIKVGIYINEEGEMIPFDKTRGYKKAYSMILSKMEEEGIKIGQKIGLIGIHCQWKRAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF95</accession>
    <name>B7IF95_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA polymerase sigma-H factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579449.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_257"/>
    <dbReference type="EnsemblBacteria" id="ACJ74759">
        <property type="protein sequence ID" value="ACJ74759"/>
        <property type="gene ID" value="THA_257"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_257"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090333_0_1_0"/>
    <dbReference type="OMA" id="LEWKVLM"/>
    <dbReference type="OrthoDB" id="1857862at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA_pol_sigma70" evidence="1">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="206" mass="24179" checksum="1F7430472080B030" modified="2009-02-10" version="1">MIKYNLRGKRIEKLVELAQSGLKEALELIIEKYYPMVIRISSKYYAPWAEFEDIVQNGLIGLIKAVFYFDNEKSSFNSFAWKSIEGEVQTFITYLNRKKNKILSDSSSIDVFDEEESGEQADYSLQDKSTNIPKKIFIDIIKETIVKHLKDVEIDIFEMWLDGYSYAEIQEELNVNFKKVDNTIQKAKRIGRTKIDRFIIEQVFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFA0</accession>
    <name>B7IFA0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_263</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579454.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_263"/>
    <dbReference type="EnsemblBacteria" id="ACJ74764">
        <property type="protein sequence ID" value="ACJ74764"/>
        <property type="gene ID" value="THA_263"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_263"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_18_0"/>
    <dbReference type="OMA" id="NTEMNTH"/>
    <dbReference type="OrthoDB" id="741876at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="6">
        <location>
            <begin position="208"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="272"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="448"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="566" mass="63460" checksum="6BD2E9F5C8CC9A58" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFB6</accession>
    <name>SYP_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proline--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="1">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">proS</name>
        <name type="ordered locus">THA_280</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579466.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFB6"/>
    <dbReference type="SMR" id="B7IFB6"/>
    <dbReference type="STRING" id="484019.THA_280"/>
    <dbReference type="EnsemblBacteria" id="ACJ74780">
        <property type="protein sequence ID" value="ACJ74780"/>
        <property type="gene ID" value="THA_280"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_280"/>
    <dbReference type="eggNOG" id="COG0442">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016739_0_0_0"/>
    <dbReference type="OMA" id="NCDYAAN"/>
    <dbReference type="OrthoDB" id="665824at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00861">
        <property type="entry name" value="ProRS_anticodon_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00779">
        <property type="entry name" value="ProRS_core_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01569">
        <property type="entry name" value="Pro_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004500">
        <property type="entry name" value="Pro-tRNA-synth_IIa_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023717">
        <property type="entry name" value="Pro-tRNA-Synthase_IIa_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044140">
        <property type="entry name" value="ProRS_anticodon_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033730">
        <property type="entry name" value="ProRS_core_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="SSF55826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00409">
        <property type="entry name" value="proS_fam_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000199436" description="Proline--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01569"/>
        </source>
    </evidence>
    <sequence length="561" mass="63962" checksum="41D6BCE0C874739A" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFC1</accession>
    <name>B7IFC1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103879.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_285"/>
    <dbReference type="EnsemblBacteria" id="ACJ74785">
        <property type="protein sequence ID" value="ACJ74785"/>
        <property type="gene ID" value="THA_285"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_285"/>
    <dbReference type="HOGENOM" id="CLU_1277114_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="216" mass="24289" checksum="A767A03E003DEF1A" modified="2009-02-10" version="1">MRKKVFLALFLTVGLFFIFSGCISLPTNKDSENEPVNLIVNGDFSEPLLFDNLTASTTTLSKWFTVVSDWAGASMDATINSNEVFQTTVNSWGITSWDYQPYWWMLQLAQFVNGATNTMYTLSFDASADTNTEMHVVVTLHAPNNPDDPNDDWWPTANLDQIIALTPDSTSFSFDVDLSGWDPWNPEEVYVKVGFEFGLSATDATIYIDNVKFIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFD2</accession>
    <name>B7IFD2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sigma-54 dependent transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_296</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103898.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_296"/>
    <dbReference type="EnsemblBacteria" id="ACJ74796">
        <property type="protein sequence ID" value="ACJ74796"/>
        <property type="gene ID" value="THA_296"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_296"/>
    <dbReference type="eggNOG" id="COG3290">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_8_1_0"/>
    <dbReference type="OMA" id="LEAKYTF"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="3"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="120"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="6">
        <location>
            <begin position="254"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="561" mass="63563" checksum="0F43F1A3A35FD4EB" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFD5</accession>
    <name>B7IFD5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methionine synthase I, cobalamin-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_299</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579481.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_299"/>
    <dbReference type="EnsemblBacteria" id="ACJ74799">
        <property type="protein sequence ID" value="ACJ74799"/>
        <property type="gene ID" value="THA_299"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_299"/>
    <dbReference type="eggNOG" id="COG5012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_495139_0_0_0"/>
    <dbReference type="OMA" id="MAIKPHI"/>
    <dbReference type="OrthoDB" id="297563at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050097">
        <property type="term" value="F:methylaspartate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019670">
        <property type="term" value="P:anaerobic glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006396">
        <property type="entry name" value="Glu_mut_E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06368">
        <property type="entry name" value="Met_asp_mut_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="SSF51703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="4" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="5">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="546" mass="61789" checksum="F9C90C5B76F24246" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFE3</accession>
    <name>B7IFE3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_307</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579486.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_307"/>
    <dbReference type="EnsemblBacteria" id="ACJ74807">
        <property type="protein sequence ID" value="ACJ74807"/>
        <property type="gene ID" value="THA_307"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_307"/>
    <dbReference type="eggNOG" id="COG2271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_0"/>
    <dbReference type="OMA" id="EIFPTKD"/>
    <dbReference type="OrthoDB" id="1121832at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="307"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="19"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="422" mass="47110" checksum="85F30FB6D10839A2" modified="2009-02-10" version="1">MKIDQVVDLIVSKKEQKNLLILTSILWIIGSAGVMVMPFVVPDLVSEWNLTTVQASSLISSVFIGMLLGALFSGIILDYFGRKIGVIFYLLVTVIFTVLFGFSKSFGSAYVFRLLSGFGYGGLLPSVNTYLSEYTSIRLRGRYLVILEASWAVGSILIALFAVTLGEKLGWRWDFYIFSIGIISLIPFFRKKDTPKYIFEKRGVEGLKKIFNKVPENIEEIEKVKVTFSGLFRKEHIKQTILVIISWFVVSFVYYALFSWAPKIFIASLGITITKAKWYTFFVYLAQLPGYLSVAYLIEKWGRKKTLATYFIGMGLSSFLLLLATGNLSFLFIVLILSFFTLGVWGLVYAYTPELFPTSFRGTANGVAGATARIAGIIAPYFTGYFVDKSIVAALSFVSLFAIFAAVMTLVLGVETKDKAVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFH6</accession>
    <name>B7IFH6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphate acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphate acetyltransferase and butyryltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104008.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_340"/>
    <dbReference type="EnsemblBacteria" id="ACJ74840">
        <property type="protein sequence ID" value="ACJ74840"/>
        <property type="gene ID" value="THA_340"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_340"/>
    <dbReference type="eggNOG" id="COG0280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056531_0_0_0"/>
    <dbReference type="OMA" id="CIVDGPF"/>
    <dbReference type="OrthoDB" id="1301815at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012147">
        <property type="entry name" value="P_Ac_Bu_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002505">
        <property type="entry name" value="PTA_PTB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01515">
        <property type="entry name" value="PTA_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000428">
        <property type="entry name" value="P_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PTA_PTB" evidence="2">
        <location>
            <begin position="75"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="303" mass="32287" checksum="02DCB44829ED15F6" modified="2009-02-10" version="1">MKKLNEILEKAKKYGPKTVAVAASEDEVVLKAIDKAKKEGIINAILVGDVEKTKKLANENNIDLSEYNLVDAKDYNDAAEKAVKLVVEKQADFVMKGKIKTGDLMRVVLKEEYGLRTGKTLSLVSIFETPLYHKLLVVSDAGMTIAPTLEQKVDLINNAVFVAKKAVGVETPKVAILGAVEIVNPKMPATIEAAILSQMNKRGQIKGCIVDGPFALDNAVSKEAAEHKGIVSDVAGDADVLIMPDIEAGNIFYKSMVFLGGAKVASAIVGAKVPVALTSRADSDETKLLSLALTTLLAGDLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFI2</accession>
    <name>B7IFI2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable succinyl-diaminopimelate desuccinylase</fullName>
            <ecNumber evidence="5">3.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">THA_347</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N-succinyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)-2,6-diaminoheptanedioate + succinate</text>
            <dbReference type="Rhea" id="RHEA:22608"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:58087"/>
            <dbReference type="EC" id="3.5.1.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.18" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_347"/>
    <dbReference type="EnsemblBacteria" id="ACJ74846">
        <property type="protein sequence ID" value="ACJ74846"/>
        <property type="gene ID" value="THA_347"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_347"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_2_1_0"/>
    <dbReference type="OMA" id="YGAKIMK"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00021"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009014">
        <property type="term" value="F:succinyl-diaminopimelate desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010182">
        <property type="entry name" value="ArgE/DapE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017706">
        <property type="entry name" value="Peptidase_M20/DapE_YgeY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01910">
        <property type="entry name" value="DapE-ArgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03526">
        <property type="entry name" value="selenium_YgeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="11">
        <location>
            <begin position="177"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ74846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="403" mass="45732" checksum="68227AB06C2D6E17" modified="2009-02-10" version="1">MNKALELAYKYKNEIVKFMSKLIKAKSYSGQEKEVVHVIKEEMEKVGFDEIKIDGLGNIIGKIGNGKYKIAMDAHIDTVDVGNEKLWEKDPFSGDFDEKWVYGRGASDQKAGMCSMVYGAKILKELGLFDDFTLYITGTVMEEDCDGLCWRYIVEKEHLKPDFVVITEPTSLNIYRGHRGRIEFRIRTTGLSAHASAPERGVNAIYKMAKIINEIEKLNDRLKSDSFLGKGTIVVSQIFFKSPSHNAVPDECEIQIDRRITEGETKETVFAEIKDVFKRAGIKDAEIIELEYKKPSYTGEIFPTEKYFPVWTFPEDSFIVQAAKKNYIDVFGKEPFIDKWTFSTNGTVTAGVYEIPTVGFGPGEERFAHAPNEKVEIEHLVKAAAFYATFPKTIVELLKTRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFK9</accession>
    <name>B7IFK9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Oligoendopeptidase F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_380</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579535.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_380"/>
    <dbReference type="EnsemblBacteria" id="ACJ74873">
        <property type="protein sequence ID" value="ACJ74873"/>
        <property type="gene ID" value="THA_380"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_380"/>
    <dbReference type="eggNOG" id="COG1164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030403_3_0_0"/>
    <dbReference type="OMA" id="CAFQFWK"/>
    <dbReference type="OrthoDB" id="1935578at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011976">
        <property type="entry name" value="Pept_M3B_oligopep-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PTHR11804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02289">
        <property type="entry name" value="M3_not_pepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M3" evidence="7">
        <location>
            <begin position="175"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="572" mass="68013" checksum="DD52B79078A86045" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFL3</accession>
    <name>B7IFL3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Aminopeptidase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_384</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579538.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_384"/>
    <dbReference type="EnsemblBacteria" id="ACJ74877">
        <property type="protein sequence ID" value="ACJ74877"/>
        <property type="gene ID" value="THA_384"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_384"/>
    <dbReference type="eggNOG" id="COG2309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054346_1_0_0"/>
    <dbReference type="OMA" id="CNANIPT"/>
    <dbReference type="OrthoDB" id="689314at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1830.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035097">
        <property type="entry name" value="M29_N-terminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000787">
        <property type="entry name" value="Peptidase_M29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02073">
        <property type="entry name" value="Peptidase_M29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00919">
        <property type="entry name" value="THERMOPTASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="398" mass="44940" checksum="F885A0616BDE307D" modified="2009-02-10" version="1">MLKKYAEVALKIGLNLQKGQILFIKAPIDAREFVEQVVETAFEIGAFDVYVKWSDEIITKTRLKNAPIDALKEFPEWEIKASEYLLDKKGAVLSITGGDPDALKDVPPERIGIFTKTVNTANKDIMRRMMSNEISWCVVAYPTRKWAKKVLGEENTEKLLEYILKASRIEGDPVKNWQEHIDNLKRITKFLNEKQFDYLKYEGPGTDLTVGLPENHIWLSATQENMSGITFVPNIPTEEIFTAPHKDKINGVVTNSLPLVYGGNVIDKFTLEFKDGKIVNYKAEVGEKILETILNTDDGAKSLGEVALVPVNSPIYKMKTIFYNTLFDENAASHFAFGRAYPNCIKDGTKMNEEQLRQNGLNTSLTHVDFMVGNENMNVYGIKAGEKITLMESGKWVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFM3</accession>
    <name>B7IFM3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hypothetical membrane spanning protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579544.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_394"/>
    <dbReference type="EnsemblBacteria" id="ACJ74887">
        <property type="protein sequence ID" value="ACJ74887"/>
        <property type="gene ID" value="THA_394"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_394"/>
    <dbReference type="eggNOG" id="ENOG503452N">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1874451_0_0_0"/>
    <dbReference type="OMA" id="WYSLIPM"/>
    <dbReference type="OrthoDB" id="1807368at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="136" mass="15635" checksum="30B0EE319D2FE41C" modified="2009-02-10" version="1">MSFLLFSIATSIILFYFTKSYLFFSIIAIGIYYLIRNNIKLQSLLSLTYVLMIALSFFSTIRGYDPKGLIFLLISCFVSILYDIFKSPIWSLPLYLLLGISISLIGSIKYGTIGYFFGLLIIPIFLKEFKKRGEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFQ1</accession>
    <name>B7IFQ1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_422</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104173.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_422"/>
    <dbReference type="EnsemblBacteria" id="ACJ74915">
        <property type="protein sequence ID" value="ACJ74915"/>
        <property type="gene ID" value="THA_422"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_422"/>
    <dbReference type="eggNOG" id="COG3246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065536_2_0_0"/>
    <dbReference type="OMA" id="VPEYEIF"/>
    <dbReference type="OrthoDB" id="1892901at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019475">
        <property type="term" value="P:L-lysine catabolic process to acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008567">
        <property type="entry name" value="KCE_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37418">
        <property type="entry name" value="PTHR37418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05853">
        <property type="entry name" value="BKACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="272" mass="30281" checksum="AB5EB97CE41E87DE" modified="2009-02-10" version="1">MEKLIITVAVTGAEVTREKQPNLPITPDEIADAVYECYLAGASIAHVHARLDDGTPTQSYEVYKEIKEKIEKKCDIIFQPSTGGATWHTFEERMQPLLTNPEMATLSAGTCNFGNDVFLNPMEYIEKFAIEMKKRNIKPEIEVFERGMIETAIKLVDKGLLNPPLHFDFVMGVPGAIPGTIEDLVYLVSKIPPGSTWSVAGIGRYELPLAVHAILMGGHVRVGFEDNIYYKKGELAKSNAQLVERIVRIAKELGREIATPDEARKILGIRKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFU7</accession>
    <name>FMT_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionyl-tRNA formyltransferase</fullName>
            <ecNumber evidence="1">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fmt</name>
        <name type="ordered locus">THA_470</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N-formyl-L-methionyl-tRNA(fMet)</text>
            <dbReference type="Rhea" id="RHEA:24380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9953"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:78844"/>
            <dbReference type="EC" id="2.1.2.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fmt family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579599.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFU7"/>
    <dbReference type="SMR" id="B7IFU7"/>
    <dbReference type="STRING" id="484019.THA_470"/>
    <dbReference type="EnsemblBacteria" id="ACJ74961">
        <property type="protein sequence ID" value="ACJ74961"/>
        <property type="gene ID" value="THA_470"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_470"/>
    <dbReference type="eggNOG" id="COG0223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033347_1_1_0"/>
    <dbReference type="OMA" id="CCPVVAY"/>
    <dbReference type="OrthoDB" id="2009156at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08704">
        <property type="entry name" value="Met_tRNA_FMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00182">
        <property type="entry name" value="Formyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005794">
        <property type="entry name" value="Fmt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044135">
        <property type="entry name" value="Met-tRNA-FMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00460">
        <property type="entry name" value="fmt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000118487" description="Methionyl-tRNA formyltransferase">
        <location>
            <begin position="1"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="106"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00182"/>
        </source>
    </evidence>
    <sequence length="304" mass="33674" checksum="FE9C9305263A58BB" modified="2009-02-10" version="1">MRILFLGTPSFASEHLEFLIKNNFDVVAVISQPDKPKGRGKKVQPTPVKEVAQKYNIPVFQPTKLTSEGLSIIERYKPDLGIVVAYGKLLKPPFLNAIPFYNIHASLLPKYRGAAPIQRALENGESVTGITIFKIGEGMDDGPIALKKEISVGEFETFGSLYEKLLSLGKEALLEFLNNYPPNLYPQEGVPTYAPKISKEDLELDFSADYVTVKNKIRAYDPIPGVRSVLKGKIVKLFQVFYVEADENIKEYGKVLKINNEGGFISAKGGIVGIKYIQFPGKKPITFLDAKNGGLVKEGDKFES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFW7</accession>
    <name>B7IFW7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Basic membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104293.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_490"/>
    <dbReference type="EnsemblBacteria" id="ACJ74981">
        <property type="protein sequence ID" value="ACJ74981"/>
        <property type="gene ID" value="THA_490"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_490"/>
    <dbReference type="eggNOG" id="COG0760">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034646_7_0_0"/>
    <dbReference type="OMA" id="AQIDMRF"/>
    <dbReference type="OrthoDB" id="1263016at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13145">
        <property type="entry name" value="Rotamase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PpiC" evidence="1">
        <location>
            <begin position="171"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="333" mass="38409" checksum="4098F0A8325D3531" modified="2009-02-10" version="1">MKRFLLLLLVVFSVVIFSEELPATSTVAVVNGEAITLEQLNRAADVQKLMIGISQVDQTFFSVLSNTEEGVKVILRYKRVVLDDLVNKLLIVQFAQKYGARLSEEEVRNVVDKQISDYLDQQGIDEKTFDMYLQYANMGTLEDFKEKMYFETLVNLSLERLFNVVTKDATVSDEELMDYYQKHIDEYSTPTQYTLSLIAFDNEKVANAVKTKIVSGETFDSIASEYGISQFKYENIAEDTTFPGKLWNYIKNAPQGALLGPIDVDEKYYIFKVENVVPMQSKKFDEVREEISNMILSEKKKNMWANFIDSEFSKFKNESTVEVYYKVDVEQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG79</accession>
    <name>B7IG79_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dexx-box atpase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_607</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104404.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_607"/>
    <dbReference type="EnsemblBacteria" id="ACJ75093">
        <property type="protein sequence ID" value="ACJ75093"/>
        <property type="gene ID" value="THA_607"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_607"/>
    <dbReference type="eggNOG" id="COG1672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041137_3_0_0"/>
    <dbReference type="OMA" id="PKYLEMF"/>
    <dbReference type="OrthoDB" id="801313at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004256">
        <property type="entry name" value="DUF234_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03008">
        <property type="entry name" value="DUF234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_14" evidence="2">
        <location>
            <begin position="25"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="DUF234" evidence="1">
        <location>
            <begin position="306"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="460" mass="54580" checksum="135C4F988C6095B6" modified="2009-02-10" version="1">MEFYNRKKELKFFDEIKSQRQKNIFIVVYGRRRIGKTTLVRKAFEKEENVFYYFVDVLNPNNLLEKTSLSFSRAVFKDWYDLFLELFGKFEFVIFDEFQNFNKVSPSILFSLQKAWDEIKYRNYNTKLIVLGSYVGLMKKLFYDYKMPLFGRKDYLVNISEFSLSESIKLLLNFGYSTKEAFEIYSLIGGIPSYLLLFKEKKSLKNHIFDLFLKDYAPLKNEGENILVLEFGSEHKSYFSILESISGSPKTISEISDLSKIEKTSLPRYLKELEEEYEIVKSIEPLFPQKKRMKKYKINDFYFDFYFNFIRKYISTIEFSPEKALELIWKNLPQYFGLKFETICQRFLKETPEVLGFIPEKIGNTWGKVPGKKNESFDIDIVAYDSENVVFGECKWTNKKVGMEDYKKLLIRSSYVDFGERNKKYILFSKAGFENELLGIKDLILFTPEGMVEKIKSHIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGE8</accession>
    <name>B7IGE8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Type I phosphodiesterase / nucleotide pyrophosphatase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579730.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_690"/>
    <dbReference type="EnsemblBacteria" id="ACJ75162">
        <property type="protein sequence ID" value="ACJ75162"/>
        <property type="gene ID" value="THA_690"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_690"/>
    <dbReference type="eggNOG" id="COG1524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039939_1_0_0"/>
    <dbReference type="OMA" id="LMNQLRW"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="375" mass="43455" checksum="0F2C2D7CC25C5044" modified="2009-02-10" version="1">MDKPVYEKSIVSFINVLLKHFKVETKYRFNEMEDIILPQIVEKEKIVVIILDSLGYKKFKELNVEFEKSIKLSSVFPTTTVAAVTSLLTAMSPQEHGLLGYIQFIREIGSIMNMIDFSYPGLPNSSFETIVKKKIKRLPNVFQSLKKEGYFGGILTSHDIVNSGLSFLIQKDANIFSYYSTIDMFANLQKLLESDFKGLLYVYYGVLDGIGHKKGPDSISYEREAKFLLEEIKKLKYDKKTQIFVVADHGMITTPREKNVFLGEELLKYLKFPPTGEMRMMYFYTKEKKDKKLIDYLSDRYSKRFEIYPSKQLLSEGYFGFGNKNLEVEHRIGDYVLLAKDNYSFTYTYTGGEQKLLGMHGSLTYEELYVPLIFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGF5</accession>
    <name>B7IGF5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Na(+)-translocating NADH-quinone reductase subunit b (Na(+)-translocating nqr subunit b) (Na(+)-nqr subunit b)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">THA_698</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579735.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_698"/>
    <dbReference type="EnsemblBacteria" id="ACJ75169">
        <property type="protein sequence ID" value="ACJ75169"/>
        <property type="gene ID" value="THA_698"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_698"/>
    <dbReference type="eggNOG" id="COG4658">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042020_1_0_0"/>
    <dbReference type="OMA" id="FNPAYPE"/>
    <dbReference type="OrthoDB" id="1654433at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004338">
        <property type="entry name" value="NqrB/RnfD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011303">
        <property type="entry name" value="RnfD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30578">
        <property type="entry name" value="PTHR30578"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03116">
        <property type="entry name" value="NQR2_RnfD_RnfE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01946">
        <property type="entry name" value="rnfD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="296" mass="32782" checksum="8B237F951F6E3B19" modified="2009-02-10" version="1">MALFQKQPMMRKVLYALIPIYIYSLFFYGVRLIWLSLFVFTFGILSEYIFEKKKGKKVSEAVLVTCMLYTLSLPPLTPWWIATIGIIFGIVFAKEAYGGFGRNIFNPAITARLFVYIAFPSYMTNGWMIPKFGSFGLDVTTSATPLALLKESQTLSLKDLFLGFRAGSIGESAIFLIIIGAIYLIFTKTASWKIIISTILSAFILTALFDIFIPSAPPTIFALLSGSLLFVSVFIATDPITAPKNSKAQVIYGIIIGVSIVIIRTFSLFAEGTSFAVLLGNTFAPFLDHVTKKVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGJ7</accession>
    <name>B7IGJ7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative glycosyltransferase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579770.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_745"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75211">
        <property type="protein sequence ID" value="ACJ75211"/>
        <property type="gene ID" value="THA_745"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_745"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_911649_0_0_0"/>
    <dbReference type="OMA" id="EGYGLYE"/>
    <dbReference type="OrthoDB" id="1445278at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="8"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="318" mass="37209" checksum="93F1761187FEF35D" modified="2009-02-10" version="1">MAPIKLLVGIPTLNRADQLKNTLHSILRNTVLPQKIIIVDQSENEKTKHIVNYFREKLNIEYLNVNYKGLTKARNEILSYAKDFDVVTFLDDDVELDKNYFEEIVKAFQEDKNLAGCQGFIENQGGIKGFLFRLAEGMPLTGSPKVTNCFVNTYPFFPPSRPVISEWLSGCNMSYRVDYIEKEKFNENFILYGLGEDLEFSYRLFVSGKRLLMLPSARLKHIPSGLKRLPSRVAVMMRLAYRRYMIAKYRSQVDMLFQEYVEWCRRTFRIHRIVRPQLWKSMMKTIEEVLNELKSHFDMIDKGDLNSVNSLILKEVSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGR7</accession>
    <name>B7IGR7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_819</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75281">
        <property type="protein sequence ID" value="ACJ75281"/>
        <property type="gene ID" value="THA_819"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_819"/>
    <dbReference type="HOGENOM" id="CLU_3141679_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="49" mass="5673" checksum="13F26979C942FB38" modified="2009-02-10" version="1">MYLNKTVDKIKNVLLNINIENTKKLLITFHGVIKLLSRKDSLFDKNGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGR9</accession>
    <name>B7IGR9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Hypothetical coenzyme PQQ synthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_821</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100422.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_821"/>
    <dbReference type="EnsemblBacteria" id="ACJ75283">
        <property type="protein sequence ID" value="ACJ75283"/>
        <property type="gene ID" value="THA_821"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_821"/>
    <dbReference type="eggNOG" id="COG0535">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_633027_0_0_0"/>
    <dbReference type="OMA" id="VFTILWE"/>
    <dbReference type="OrthoDB" id="1172757at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="62"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="433" mass="50358" checksum="52B1D84F6708F096" modified="2009-02-10" version="1">MKRFFADQKDNYCLCFDSVTLKWIIGEKKLSLSCSNDDRFLIEIFESEDTEDNRLENLTNNLSENNFIGMSILPTFECNLKCLHCFYESGPNKHFVALDEKYIPLIIDFVNENNIENITIGGGEPLIWRHLRKFLEEVLEKTNARIFLLTNGLMINLIEDLLRSSRFNVQISVDAARRETYKRIRGGDFEKLMKNIVKLKEIGVDVALSYTIHSYNKAEVVDFIKKAELVEVNSIHFPIIERYGRAKSNDIIPDSKKLTDLYQFLIYYSFHYNKIKIYFVEEIKLRLLRRITRHNCSALYNQLSLASDGYFYPCSELINDRFKICHISEYKNLKEIIKKFKTRTGIGTPVVEKICPECPIKLLCGGGCRAVELLSGKSFVEAKAESLVCDIFIKSVFTILWELANYELNPPVPIDLLYSKEVLRLVEKYEKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGT3</accession>
    <name>B7IGT3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pullulanase</fullName>
            <ecNumber evidence="3">3.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Alpha-dextrin endo-1,6-alpha-glucosidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Pullulan 6-glucanohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pulA</name>
        <name evidence="7" type="ordered locus">THA_835</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Hydrolysis of (1-&gt;6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.</text>
            <dbReference type="EC" id="3.2.1.41"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.41" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579824.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_835"/>
    <dbReference type="CAZy" id="CBM41">
        <property type="family name" value="Carbohydrate-Binding Module Family 41"/>
    </dbReference>
    <dbReference type="CAZy" id="CBM48">
        <property type="family name" value="Carbohydrate-Binding Module Family 48"/>
    </dbReference>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75297">
        <property type="protein sequence ID" value="ACJ75297"/>
        <property type="gene ID" value="THA_835"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_835"/>
    <dbReference type="eggNOG" id="COG1523">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004744_1_0_0"/>
    <dbReference type="OMA" id="KMMRKFM"/>
    <dbReference type="OrthoDB" id="99080at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051060">
        <property type="term" value="F:pullulanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10315">
        <property type="entry name" value="CBM41_pullulanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005323">
        <property type="entry name" value="CBM41_pullulanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011840">
        <property type="entry name" value="PulA_typeI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045041">
        <property type="entry name" value="Pullulanase_PULA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43631">
        <property type="entry name" value="PTHR43631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03714">
        <property type="entry name" value="PUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="SSF49452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02104">
        <property type="entry name" value="pulA_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="6">
        <location>
            <begin position="371"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="844" mass="96661" checksum="36D4EF071DAFC104" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGT7</accession>
    <name>B7IGT7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_839</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100458.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_839"/>
    <dbReference type="EnsemblBacteria" id="ACJ75301">
        <property type="protein sequence ID" value="ACJ75301"/>
        <property type="gene ID" value="THA_839"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_839"/>
    <dbReference type="eggNOG" id="COG1277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064090_0_0_0"/>
    <dbReference type="OMA" id="HTIEGFV"/>
    <dbReference type="OrthoDB" id="1376978at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032688">
        <property type="entry name" value="ABC2_membrane_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="265" mass="30849" checksum="C0ACD2C3DF2729D3" modified="2009-02-10" version="1">MNILKKEINQNIIVILIWMIVISLFIWMFSAATTFFLDENTPALKIIQNMPEKLLKTFNIELDVLSKPEGIFGTEGMTFMFIFFGIFSSIIASKIFAGEFDNKTIEYLLVKPVSRTKTFLSKIAATILFITILSFTFLFSEHIFFPLYVKNFSKLVMNYFFIYIFISSIFFASFSTLLSMIFRKRKLVNTISISFVFLMFFLNSVTEGVENFEFLRKISIFYYFSTIDILKELTINYSAVAAIIFISTILFISSHYIFKNNDISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGU1</accession>
    <name>B7IGU1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Trehalose/maltose binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_844</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579827.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_844"/>
    <dbReference type="EnsemblBacteria" id="ACJ75305">
        <property type="protein sequence ID" value="ACJ75305"/>
        <property type="gene ID" value="THA_844"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_844"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_9_1_0"/>
    <dbReference type="OMA" id="PFWANTQ"/>
    <dbReference type="OrthoDB" id="1303718at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="414" mass="46770" checksum="2F9F506147F7B3B2" modified="2009-02-10" version="1">MKRLFVLFSLLLVFALSFSITTITMTSGGVGKELEVLYAQLKEFMKQNPDIVVTVIPMPDSSTERHDLYVTYLAAGESDPDVLMLDVIWPPEFAPFLEDLTDDYDYFELDKFLPGTVKSVTVDGKIVAVPWFTDAGLLYYRKDLLEKYGYDHPPQTWDELVEMAQKISKAEGIEGFVWQGARYEGLVCDFMEYVWSFGTDVLDENGNVIINNPKAIDALKFMVDLIYKYRISPEGVTTYMEEDARRIFQTGNAVFMRNWPYAWSLANSDDSPIKGKVGIAPLPKGPGPDGKHAATLGGWNLGINVYSSPKEKEAAKKLIKFLTSSEQQLYKAVNAGQNPTRKEVYNKPELKEANPFMVELFDVFINALPRPRTASYAEVSDAIQRYVHAALTRELTPEQAISRLEKELKMLLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGW1</accession>
    <name>B7IGW1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase, group 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579845.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_865"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75325">
        <property type="protein sequence ID" value="ACJ75325"/>
        <property type="gene ID" value="THA_865"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_865"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_752130_0_0_0"/>
    <dbReference type="OMA" id="IGYMHPK"/>
    <dbReference type="OrthoDB" id="1591999at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="1">
        <location>
            <begin position="69"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="363" mass="43327" checksum="717ADD5A2F4A299E" modified="2009-02-10" version="1">MKIFIIGYMHPKYDKRVFRTVKALSKDNEVIYQYWTDKEEIESKEGNIFYKPIKYEKNVKANPILKLMRRRKLDKKIVEMIKNYDYDIVYFHHFLASRPLAPFKVARRRDKVVIYDIHEYHPENFLNILKGFAKVLKEKIMWKIFERQLKLSDKLIFVSKETQKDVYKRLGIDKPYLIAPNYASISVESREKKKEISFVGKVTRNLANEKKIIKKLIERGFKFKIIGMDSDVFKDIPHEYTTFLPYEDMMEQLSKSAFSLISYNTIMDESYKNDIFALPNKYYDSIAAETPVIVKNTFVSMAKEVEKYGIGVVIDPNNVDKSVEKILKAYENYENLLENIKKYKDRFVWNEKKEKEFISFVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGY5</accession>
    <name>B7IGY5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribonuclease H-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579864.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_890"/>
    <dbReference type="EnsemblBacteria" id="ACJ75349">
        <property type="protein sequence ID" value="ACJ75349"/>
        <property type="gene ID" value="THA_890"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_890"/>
    <dbReference type="eggNOG" id="COG0328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030894_2_2_0"/>
    <dbReference type="OMA" id="DYNEIPM"/>
    <dbReference type="OrthoDB" id="1953872at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="147" mass="17285" checksum="5B12929FB09513CF" modified="2009-02-10" version="1">MQIYVDGSYYDDLNIAGFSFCVVENEEIKFTYKDAMEINRGNSIIAELLGVIKALEYCNNLGVKEVTIIHDYNEIPMLASSYRNTKNPYINSYARKLRQFCQQLQVSFVKVKAHKNDRFNNYVDEMSRKSVEEYLIKRLKKSKYYKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH22</accession>
    <name>B7IH22_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pyrophosphate--fructose 6-phosphate 1-phosphotransferase</fullName>
            <ecNumber evidence="8">2.7.1.90</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">6-phosphofructokinase, pyrophosphate dependent</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">PPi-dependent phosphofructokinase</fullName>
            <shortName evidence="8">PPi-PFK</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Pyrophosphate-dependent 6-phosphofructose-1-kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pfp</name>
        <name evidence="10" type="ordered locus">THA_927</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose 1,6-bisphosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13613"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="2.7.1.90"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="8">Non-allosteric.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade 'B2' sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.90" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579884.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_927"/>
    <dbReference type="EnsemblBacteria" id="ACJ75386">
        <property type="protein sequence ID" value="ACJ75386"/>
        <property type="gene ID" value="THA_927"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_927"/>
    <dbReference type="eggNOG" id="COG0205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020655_1_1_0"/>
    <dbReference type="OMA" id="AHDIGYF"/>
    <dbReference type="OrthoDB" id="1421302at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047334">
        <property type="term" value="F:diphosphate-fructose-6-phosphate 1-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01978">
        <property type="entry name" value="Phosphofructokinase_II_B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011404">
        <property type="entry name" value="PPi-PFK_XF0274"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036483">
        <property type="entry name" value="PFK_XF0274"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="SSF53784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PFK" evidence="9">
        <location>
            <begin position="6"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="129"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="177"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="283"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP" evidence="8">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi" evidence="8">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="395" mass="44221" checksum="68AA32E3FF95D8DD" modified="2009-02-10" version="1">MKVLYAQSGGVTSVINASAYGVLDEAKKAGLEVYVGIHGISGVLRENLLRINDIDIEGLKYTPSAAFGSCRRKLKNQEDINKLFEIFEKYEIEYFFYNGGNDSMDTAWRLHNEAQKRNFPLKVIGVPKTIDNDLPYTDHCPGYGSAAKYIATAMMEATLDLRSMYADSTRVFVMEIMGRHAGWLAAAAGLGWLNGIGPDIILFPEVVFNKERFLDKVDEKIRRKGYCSIAVSEGIKYEDGFFVADMGYTDSFGNRQLGGVGFTIAGMVRSELSLKTHVAIPDYLQRSGRHIASKTDVEEAEGVGREAVRLALSGVSGVMVTIERISNDPYKVEFKTVELNKVAEQTKYLPEDFHNEFEVTDKFIKYAKPLIEGEFFPPFKNGLPDYLIIEEVEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH26</accession>
    <name>B7IH26_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="14">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="14">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 19" type="primary">pheT</name>
        <name evidence="19" type="ordered locus">THA_931</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="14"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579886.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_931"/>
    <dbReference type="EnsemblBacteria" id="ACJ75390">
        <property type="protein sequence ID" value="ACJ75390"/>
        <property type="gene ID" value="THA_931"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_931"/>
    <dbReference type="eggNOG" id="COG0072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016891_2_0_0"/>
    <dbReference type="OMA" id="ISYNWLK"/>
    <dbReference type="OrthoDB" id="53568at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02796">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00283">
        <property type="entry name" value="Phe_tRNA_synth_beta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004532">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033714">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PTHR10947"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="SSF54991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00472">
        <property type="entry name" value="pheT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 14 19" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="16">
        <location>
            <begin position="39"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="B5" evidence="18">
        <location>
            <begin position="393"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="17">
        <location>
            <begin position="687"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ACJ75390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="782" mass="89150" checksum="697429B5B675426C" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH73</accession>
    <name>B7IH73_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Na+ driven multidrug efflux pump</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579911.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_978"/>
    <dbReference type="EnsemblBacteria" id="ACJ75437">
        <property type="protein sequence ID" value="ACJ75437"/>
        <property type="gene ID" value="THA_978"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_978"/>
    <dbReference type="eggNOG" id="COG0534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_5_1_0"/>
    <dbReference type="OMA" id="FYWRMVS"/>
    <dbReference type="OrthoDB" id="1415498at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="445" mass="49444" checksum="C21F9F526270838C" modified="2009-02-10" version="1">MGIYKKIFAIALPIALQQFLSTGVNFVDNLMIGKLGEVAIASVGLSNQFFFLYNLILFGLISGGSIFFSQFWGKKDLDGIAKSSAVTSATALLFSAVFFTLSFFFPQTVMRFFSPDPLVISEGIIYLKIISFSFPIFALSMVFSFVLRSVEKAHIPMYVTIVELSTNVFLNYSLIFGKFGFPKLGILGAAIATLIARIVGLVSLILIIKVKNLPGFFTLKHIEMIDKKFLKNFFHYTLPTIANEFAWSFGMTMYSVIYAHMSTQTIAARNIMGTIEGFAYSFTFSIASAASVIVGNYLGASRFEEAFDVSKKVIKLSQIVAIISGTLTVILSYYIVNFFDVSDEVKMLVRVTITISMAFIPIKVFNGLNIVGFLRAGGDTRYSFMVEAGTLWLLGVPFAAFSGLVLKFSLPIVYLFTMSDEIAKAIILYSRYHSRKWVKNVVEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH74</accession>
    <name>B7IH74_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_979</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100953.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_979"/>
    <dbReference type="EnsemblBacteria" id="ACJ75438">
        <property type="protein sequence ID" value="ACJ75438"/>
        <property type="gene ID" value="THA_979"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_979"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_6_1_0"/>
    <dbReference type="OMA" id="TIWYSIA"/>
    <dbReference type="OrthoDB" id="1772343at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="87" mass="9954" checksum="83D305D073D217B2" modified="2009-02-10" version="1">MEKFEEAASILKALGHPIRLKILYLLSEKEHCVCELLSQINTSQPNLSQHLSILRNLKLIKDERNGNMVIYKLQDNKIVKAILSALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHE0</accession>
    <name>B7IHE0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">N(1)-alpha-phosphoribosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cobT1</name>
        <name evidence="10" type="ordered locus">THA_1046</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5,6-dimethylbenzimidazole + nicotinate beta-D-ribonucleotide = alpha-ribazole 5'-phosphate + H(+) + nicotinate</text>
            <dbReference type="Rhea" id="RHEA:11196"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15890"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="EC" id="2.4.2.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-ribazole from 5,6-dimethylbenzimidazole: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CobT family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.21" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579955.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1046"/>
    <dbReference type="EnsemblBacteria" id="ACJ75504">
        <property type="protein sequence ID" value="ACJ75504"/>
        <property type="gene ID" value="THA_1046"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1046"/>
    <dbReference type="eggNOG" id="COG2038">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002982_0_0_0"/>
    <dbReference type="OMA" id="AIWYAGW"/>
    <dbReference type="OrthoDB" id="1765140at2"/>
    <dbReference type="UniPathway" id="UPA00061">
        <property type="reaction ID" value="UER00516"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02439">
        <property type="entry name" value="DMB-PRT_CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1610.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003200">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017846">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023195">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02277">
        <property type="entry name" value="DBI_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="SSF52733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03160">
        <property type="entry name" value="cobT_DBIPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="313" mass="34354" checksum="9E4D3FD09539CFD7" modified="2009-02-10" version="1">MNEAGYLEELASKLFKIKKQIPLPMFKDKRVYVFAADHGVVQNGVSAYPKNVTYQMVLNYFKGGAGINVFARHMGLKFFVVDSGVDYDFEDNPSLIKMKVGYGTKDFSMGPAMTREEAITCIEYGMKVAKKAISQGADLLVIGDKGIGNTTTATAIFASFGFDVNKITDIGTPIPPSSVIKKRNIVKKALKINRPNPNDPIDVLSKVGGYCIGQMAGFILEAFKNKVPVVIDGFPTTAGFYLAYKINKKVLDYAFFGHLSKVKGHKVILDSLGVRPILDLDMRLGEGTGAALSVYLIEAAIKYFNFKNEKVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHF6</accession>
    <name>B7IHF6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">N-acetylglucosamine-6-phosphate deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1064</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="6">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the metallo-dependent hydrolases superfamily. NagA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579971.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1064"/>
    <dbReference type="EnsemblBacteria" id="ACJ75520">
        <property type="protein sequence ID" value="ACJ75520"/>
        <property type="gene ID" value="THA_1064"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1064"/>
    <dbReference type="eggNOG" id="COG1820">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032482_2_1_0"/>
    <dbReference type="OMA" id="HAFNAMP"/>
    <dbReference type="OrthoDB" id="1026967at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008448">
        <property type="term" value="F:N-acetylglucosamine-6-phosphate deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00854">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003764">
        <property type="entry name" value="GlcNAc_6-P_deAcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF14">
        <property type="entry name" value="PTHR11113:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038994">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00221">
        <property type="entry name" value="nagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="7">
        <location>
            <begin position="39"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="197"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="284"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR038994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="350" mass="39310" checksum="CA13A2EEFA38E614" modified="2009-02-10" version="1">MIIDNVLIVDPIDGEFTGSIEFEDKILSIRKYDCTPKYVLMPGFADTHTHGFMGIDCMQSTKEEFKQWADLVEKEGVTYLFPTTVSADFKMLEKVIKKFSSAKHSSLFYLHLEGPFINKERAGAQNKKYITDFDGKIIDLERIKIMTIAPEIKGFFELLKILKENKVTVSIGHSNATFDIFKKAYEAGVNRITHFPNALPQLHHREIGIVGGVFLYKPFVEIIPDLIHLSQDFLRLVYKLLGPDRIILVTDSISATGLKDGIYDLGGLLVKVKDGIARLEDGTLAGSTLKYSDGLRNFKKVTNCSLKDLSKVSSYNALKSVGIDGGRIKEGYPAKFVLLNQDLQIIKVYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHH0</accession>
    <name>B7IHH0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uroporphyrinogen-III C-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1078</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.107" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579984.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1078"/>
    <dbReference type="EnsemblBacteria" id="ACJ75534">
        <property type="protein sequence ID" value="ACJ75534"/>
        <property type="gene ID" value="THA_1078"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1078"/>
    <dbReference type="eggNOG" id="COG0007">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1587">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_6_0_0"/>
    <dbReference type="OrthoDB" id="1185397at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="SSF69618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="7">
        <location>
            <begin position="4"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="HEM4" evidence="8">
        <location>
            <begin position="251"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="462" mass="52525" checksum="D21523D299E54C17" modified="2009-02-10" version="1">MGVVYLVGAGPGDLKLITLKGLEVLKKADCVVYDHLINPNLLNFAKDDAKLIYVGKVAGNHSKTQEQINKILEDEARKANVVVRLKGGDPFVFGRGGEEFDYLTQRGIKVEIIPGITSAIAAPAYAGIPVTQRGISSHFHVYTGHSKDTKIYEDSGTIVFLMVSKNIDFVKEKLLEKFLPDTPCAVINNGTTNLQKTYLTSIKDLEERLFDSPSLLVVGNVVKKRINWFEGGKLFRKKILITTQYSKDFEILEKSGAQLYFVPTFYIKENVEKLEYLAKNLDKYETLIFTSKNGVRLFLKWLKKFKIDLRGLKAEIAVIGEKTAKEFEKVGVFADIIPEKELSEKLFEMIDKRKKAAYITSTLGKNINEENIEKIEVYQTIENYRMKRVLNEVLKKEMDYVVFTSPSSFLFFLKMAKLNKEKICAIGPVTKKCIEDHGYKVEIMPKKYTYEELCKEILKKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHI1</accession>
    <name>B7IHI1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1089</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101178.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75545">
        <property type="protein sequence ID" value="ACJ75545"/>
        <property type="gene ID" value="THA_1089"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1089"/>
    <dbReference type="HOGENOM" id="CLU_2738703_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="71" mass="8584" checksum="33337B1FBFF05580" modified="2009-02-10" version="1">MFYNKENLFVPYDFARFLLEKNFELRDSIYIPIYITLENSVDISRFLRKGDVLSKKDNTFLVLLEKIIIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHI4</accession>
    <name>B7IHI4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579995.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1092"/>
    <dbReference type="EnsemblBacteria" id="ACJ75548">
        <property type="protein sequence ID" value="ACJ75548"/>
        <property type="gene ID" value="THA_1092"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1092"/>
    <dbReference type="eggNOG" id="COG4907">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026556_1_0_0"/>
    <dbReference type="OMA" id="EHPPESI"/>
    <dbReference type="OrthoDB" id="556665at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018702">
        <property type="entry name" value="DUF2207_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09972">
        <property type="entry name" value="DUF2207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="580" mass="66998" checksum="57E9C241E3A2053A" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHJ9</accession>
    <name>B7IHJ9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methyl-accepting chemotaxis protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1110</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1110"/>
    <dbReference type="EnsemblBacteria" id="ACJ75563">
        <property type="protein sequence ID" value="ACJ75563"/>
        <property type="gene ID" value="THA_1110"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1110"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_18_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="Methyl-accepting transducer" evidence="3">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="27"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="169" mass="18299" checksum="AF725329084D73F8" modified="2009-02-10" version="1">MLALNAAIEAARAGEAGKGFAVVADEIRKLAEESKNATTQISEILKDIQEKIENTAKASNQTVEIIEEASNQSKIAKEKFENILRQIESITDEIDNLAASSQQQSASAQEMSSAMDVVSKAIVTTSQQVDDIAKSAQSQAKTSEKLDISSENLNKIAKDLVEEVKKFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHK9</accession>
    <name>B7IHK9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580012.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1120"/>
    <dbReference type="EnsemblBacteria" id="ACJ75573">
        <property type="protein sequence ID" value="ACJ75573"/>
        <property type="gene ID" value="THA_1120"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1120"/>
    <dbReference type="HOGENOM" id="CLU_1282705_0_0_0"/>
    <dbReference type="OMA" id="QIAKTCE"/>
    <dbReference type="OrthoDB" id="1968844at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="211" mass="25183" checksum="B7654CEE292F20C9" modified="2009-02-10" version="1">MLEVIFTLIFIVLFRFIILKFLGYDRKSILLRDSEFWSLLIFVGIALSIFFDKSNYLLYILVLLGILMNVFNYISYRLTFASMLSRIFIFLILLFPKYIWIVSIFAIFSIYRMKRTPIYNWIYNSKVAEQIAKTCEKGEYTRKPIIVFAPFNRSAIFYGKGFTVNVKEDKAIFRISRKTHRLLGFPKLSEFCEKFSEYIRGWLNDKGKEFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHL5</accession>
    <name>B7IHL5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thermostable monoacylglycerol lipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580016.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1126"/>
    <dbReference type="ESTHER" id="theab-b7ihl5">
        <property type="family name" value="CarbLipBact_2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75579">
        <property type="protein sequence ID" value="ACJ75579"/>
        <property type="gene ID" value="THA_1126"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1126"/>
    <dbReference type="eggNOG" id="COG1647">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076594_0_0_0"/>
    <dbReference type="OMA" id="DWYAEVD"/>
    <dbReference type="OrthoDB" id="1694750at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012354">
        <property type="entry name" value="Esterase_lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017388">
        <property type="entry name" value="Esterase_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="2">
        <location>
            <begin position="29"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR017388-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="258" mass="29727" checksum="DA6982E4D61392FE" modified="2009-02-10" version="1">MNLLKFSTKIKTVEASQPIFLEGSNGIGVLLIHGYTGSPHDMKFLAEKLNKEGFTVYVPRLPGHGTSSEDFLKSNHRDWLRRVIDSYFDLSGKCKEVYVAGLSMGGVLTLILASIFNPKKIVSIAGAIFTTEKKIALTPYISLFLKKMKREDYNETYEDKDLQYLSKEYWSYNWPLQAKYLYKLMKIARKRLKYINSDILILASEKDLTVPLEAAHYIYNNVSSKNKELKIFKKTGHVMTNDIEKEEVAETIIEWFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHM8</accession>
    <name>B7IHM8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580028.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1140"/>
    <dbReference type="EnsemblBacteria" id="ACJ75592">
        <property type="protein sequence ID" value="ACJ75592"/>
        <property type="gene ID" value="THA_1140"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1140"/>
    <dbReference type="HOGENOM" id="CLU_841815_0_0_0"/>
    <dbReference type="OrthoDB" id="244741at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="330" mass="36947" checksum="6E22BA90446A647D" modified="2009-02-10" version="1">MKRIKFVFLVFLVIFILFSCGINVPKKLPETIKVKYKNHVEIPLTTLHYELNNFTTSMVESLENSGFKITSETPITVSTENTFEISPKIETPIEGISGDATFNVFDKTTILNFSIISDSSPLEMLSFDNVEFKIYFESTTTNLDSTLTFYLNDSKIILTKNSSEANLSATLKNALENKKNLEASATIYVKGTPGDNDEFGIKYFVKIPLSGTVKTDYRIISQEVDLTSVATILENIESAYIVFKEWKNQTGLSKIGFKLGNLSSFIDDSNTKISITKQDFADITKQKTLLEIFIPKDSSIQINSSSYIDASSYLSLDLNLENVEFTLGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHN6</accession>
    <name>B7IHN6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UDP-N-acetylmuramate--L-alanine ligase</fullName>
            <ecNumber evidence="4">6.3.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">murC</name>
        <name evidence="14" type="ordered locus">THA_1148</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-alanine + UDP-N-acetyl-alpha-D-muramate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine</text>
            <dbReference type="Rhea" id="RHEA:23372"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="ChEBI" id="CHEBI:83898"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.3.2.8" evidence="4"/>
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        <property type="protein sequence ID" value="ACJ75600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1148"/>
    <dbReference type="EnsemblBacteria" id="ACJ75600">
        <property type="protein sequence ID" value="ACJ75600"/>
        <property type="gene ID" value="THA_1148"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1148"/>
    <dbReference type="eggNOG" id="COG0773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028104_2_2_0"/>
    <dbReference type="OMA" id="DITYQLR"/>
    <dbReference type="OrthoDB" id="307881at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0008763">
        <property type="term" value="F:UDP-N-acetylmuramate-L-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005758">
        <property type="entry name" value="UDP-N-AcMur_Ala_ligase_MurC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01082">
        <property type="entry name" value="murC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="11">
        <location>
            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="13">
        <location>
            <begin position="105"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="12">
        <location>
            <begin position="292"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ75600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="433" mass="48675" checksum="9A47CA4C3F7F6647" modified="2009-02-10" version="1">MKIHFLGIGGIGMSAQAIHEHFSGNVVSGTDPYSSERVNYLKSLGIQVFKSHLPENIDNADLIVRTPAVSEDNVEVKKAKENGIPVISRLEHHISILRPYTKIGVTGTDGKTTTTAMLAHVLLKLGKDPTVFLGSTHPMLEHGNYRKGSNIAVFEMDESQPGFENYNPDYLIITNIRGDHIENFKNLQHYNSCFENSCKNVKICVSNVENNIINNSIKFGLNDGDYKINKIENNKFSQKITINTPYGKKKFILNVPGVHNALNALSIISLLFEMGYEKDLVLEAFSDFSLPKRRFDISYNENDIVIIDDYSHTPVEIKSLLSTAKSVFKDKKITIIFQPHRYSRLKREWKNFSEALSLADKIYITEVYGAFESKNGISARLIVEKINNAIFVQEKEELLQILPSEPGVYIFAGAGDIIEISKKFKEKVGGLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHS1</accession>
    <name>B7IHS1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ggdef domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1185"/>
    <dbReference type="EnsemblBacteria" id="ACJ75635">
        <property type="protein sequence ID" value="ACJ75635"/>
        <property type="gene ID" value="THA_1185"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1185"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_495878_0_0_0"/>
    <dbReference type="OMA" id="NRENENF"/>
    <dbReference type="OrthoDB" id="306887at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="428"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="551" mass="64124" checksum="86D804C319DFE418" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7II00</accession>
    <name>B7II00_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580106.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1269"/>
    <dbReference type="EnsemblBacteria" id="ACJ75714">
        <property type="protein sequence ID" value="ACJ75714"/>
        <property type="gene ID" value="THA_1269"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1269"/>
    <dbReference type="eggNOG" id="ENOG502ZACD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_705703_0_0_0"/>
    <dbReference type="OMA" id="WINWIFL"/>
    <dbReference type="OrthoDB" id="1169193at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="400" mass="46864" checksum="50A4074F4BC041F7" modified="2009-02-10" version="1">MERKTLYSFLELSIINLIIFILDLLFKNYGYITVNPNPYLILNLYVAIRYGTNIFFASAALSTFFYFLSLHIQYGLNVYLILFSWPVLKIPLFILSLGFLIAFFRDNYVQTINEQEEKIKILSKKIETLEDKIRKYENLTNDLQNKLLLENKGVSIFVDKLKDIEYNNPEDIFNEAVELIYDFIEAKTVSIYTLSNNDFLRLKVRKGPQILPNSFPLEKSKVLSTAKDLGFASASILYLSNVDIDFSFEPAMASKIEHTGKILGFIVIENIDPEKINKNTETYIKILSDWLSNMLYVSSELEEQIIKNDLEKFNFIFEKIEERFERFKIPYSFIKAKIKENSDIENIKKFIRETDFVFVDEKNSELKIVLTSCNSKGLKRVLDKLKDFKQIEIEEAYTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7II03</accession>
    <name>B7II03_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ggdef domain/hd domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1272</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580109.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1272"/>
    <dbReference type="EnsemblBacteria" id="ACJ75717">
        <property type="protein sequence ID" value="ACJ75717"/>
        <property type="gene ID" value="THA_1272"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1272"/>
    <dbReference type="eggNOG" id="COG2206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_92_14_0"/>
    <dbReference type="OMA" id="KWVRHHH"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="HD-GYP" evidence="3">
        <location>
            <begin position="246"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="268"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="435" mass="49801" checksum="11F985873239F75F" modified="2009-02-10" version="1">MKKKGVALDLKESLVHIYTLALGAIYVALLFYAIKKYGFDYSSIDFYLFFLFSLFFEFIPVRFQTFFENKFDEKYKEHDLYLSAGLIVSVLSAIHLNISNALIIPFLVFLVLRSNVLFSKNYYYFIFNVSSIGILVFLSFKVYNVFPNVVKNSLILSSFVVALISIFYFVLNLSFVLTFLTLVYRRFSKDVIFKLIGEGRLTNLFSTSINTFIVYILYKFIGISAIPVSFFTIIGIQLGNYYATKYRQAKLDLLTALAKSLEEKDSYTYGHGENVAKISVAIAKELGIQDKELNLIEIAGLLHDVGKIGIPDFIVTKTGKLTRDEYEIMKEHPKKGYEILSQITEFKDTVAKWVLHHHERWDGKGYPEGISGEEIPLESRILMIADVYHALTSDRPYREAWSKEQAIGFIKDNAGIMFDPKIVNIFLKLIEGGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICJ7</accession>
    <name>B7ICJ7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1279</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1279"/>
    <dbReference type="EnsemblBacteria" id="ACJ75724">
        <property type="protein sequence ID" value="ACJ75724"/>
        <property type="gene ID" value="THA_1279"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1279"/>
    <dbReference type="eggNOG" id="COG3437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_92_3_0"/>
    <dbReference type="OMA" id="HMSRELA"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD-GYP" evidence="2">
        <location>
            <begin position="33"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="233" mass="27310" checksum="346CC1D249361C0D" modified="2009-02-10" version="1">MAEELVAYMQELNAMNEELETSYREIERMSNELEHSYIDFSMRLARVAEGYDENTGNHIERVGILAAFIAEKLGLSKKIAYYLRYHAPLHDIGKIFIPREILMKKGNLTKEEWEEMKKHTIYGARLIGDNIQFEVARNIALYHHENYDGTGYPYGLEKSEIPIEAMIVHIVDVYDALRSERPYKKSFTHEEAMKIILEGDGRTQPSHFAPEVLEVFKRYESEIEKLWNVIYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICK2</accession>
    <name>B7ICK2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580118.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1284"/>
    <dbReference type="EnsemblBacteria" id="ACJ75729">
        <property type="protein sequence ID" value="ACJ75729"/>
        <property type="gene ID" value="THA_1284"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1284"/>
    <dbReference type="eggNOG" id="ENOG50337CQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117668_0_0_0"/>
    <dbReference type="OMA" id="PTERENN"/>
    <dbReference type="OrthoDB" id="1554688at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032604">
        <property type="entry name" value="DUF4897"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16238">
        <property type="entry name" value="DUF4897"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="190" mass="21864" checksum="3BFC5D37687CA753" modified="2009-02-10" version="1">MQNKTFMYILILVVIVFLIFDLFTVFNRRPKFDITFYKTVVETDYSDTATITTIAGLSFKDEKSMYEYKESYTNASSKTFLNYFEQISKEIGKTITVVSYENSAKERAGILEIEEVAVLKNLVDVSNNTYTLNMGNIQINPNPNSLFIVYIPKDSILVSSNPTPTSFENNKLSWDGESLKNFPTVIYRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICK8</accession>
    <name>B7ICK8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alpha-mannosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1290</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580123.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1290"/>
    <dbReference type="CAZy" id="GH38">
        <property type="family name" value="Glycoside Hydrolase Family 38"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75735">
        <property type="protein sequence ID" value="ACJ75735"/>
        <property type="gene ID" value="THA_1290"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1290"/>
    <dbReference type="eggNOG" id="COG0383">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003442_1_2_0"/>
    <dbReference type="OMA" id="GQYWDAW"/>
    <dbReference type="OrthoDB" id="299689at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="SSF88688"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-mann_mid" evidence="5">
        <location>
            <begin position="506"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="997" mass="116678" checksum="562EF7B6E09FD2A5" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICL4</accession>
    <name>B7ICL4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sugar ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1296</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580127.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1296"/>
    <dbReference type="EnsemblBacteria" id="ACJ75741">
        <property type="protein sequence ID" value="ACJ75741"/>
        <property type="gene ID" value="THA_1296"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1296"/>
    <dbReference type="eggNOG" id="COG2182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_10_0_0"/>
    <dbReference type="OMA" id="QNAAWEL"/>
    <dbReference type="OrthoDB" id="1303718at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="422" mass="47540" checksum="BAFC1A772B171166" modified="2009-02-10" version="1">MKKFLSVIIAVLVVSILFSQVEVVFWHGLSRDPDKSSIDKIIAEFEKENPDIKVKVVLVPAAETDSTKLLTAVAAGTGPDLVYLDRFTVSQRAANNVLEPMEDYLKMAGINIEEMKKQFYDFAIEECIFNGKMWALPFDTDVRILLYNKKLLEKAGYSKPPKTVYEMIDVAEKLTVEGKRGRYETVGFIPWYAQGWPYTWIYAFEGKVFDKKTGKFVFATDPGVIEAYKWQKEWADRFGYEALNAFGSLQIGDLNPFTAGKLAMIVDGNWTISGLRMFAPKDFKYEITGIPTKSGHPVTWAGGWSLAIPKGAKNKEAAAKLAYFIATKGQVIYSVDTLHLPTYKPAVDDFLKKDPSQKVFVDLLDHAKTRPPLPVGALLWDKLVEARDFVLTGQKSVEQALLDAQKEVQEEYDRIMQRVGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICL8</accession>
    <name>B7ICL8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator of phasin expression</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580131.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1300"/>
    <dbReference type="EnsemblBacteria" id="ACJ75745">
        <property type="protein sequence ID" value="ACJ75745"/>
        <property type="gene ID" value="THA_1300"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1300"/>
    <dbReference type="eggNOG" id="COG5394">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1666795_0_0_0"/>
    <dbReference type="OMA" id="QGFIKIF"/>
    <dbReference type="OrthoDB" id="1665699at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012909">
        <property type="entry name" value="PHA_DNA-bd_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07879">
        <property type="entry name" value="PHB_acc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHB_acc_N" evidence="1">
        <location>
            <begin position="3"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="161" mass="18546" checksum="1CC536DB98429756" modified="2009-02-10" version="1">MRIIKKYKNRKLYDTSIKKFITLSDIANFVKNGEAVKVIDNLGNDITQEIFLKARIKGRVFGSLKKEMVEKLIQGFIKIFKGNTDDFIEILLDLVNQGVLPADIAKELGNAVIRHFNEFEDNMKKDIIELIKSTGFVPKEEYDKLKKEYELLKKKCEGQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICM9</accession>
    <name>B7ICM9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 7">30S ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">rpsI</name>
        <name evidence="8" type="ordered locus">THA_1311</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101630.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1311"/>
    <dbReference type="EnsemblBacteria" id="ACJ75756">
        <property type="protein sequence ID" value="ACJ75756"/>
        <property type="gene ID" value="THA_1311"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1311"/>
    <dbReference type="eggNOG" id="COG0103">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046483_2_1_0"/>
    <dbReference type="OMA" id="QWKINGR"/>
    <dbReference type="OrthoDB" id="1766414at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_B">
        <property type="entry name" value="Ribosomal_S9_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_S9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_S9_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="PTHR21569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="132" mass="14931" checksum="E2253FDA07C0DB91" modified="2009-02-10" version="1">MAEYYMGTGRRKTSVARVYLKEGNGKVTVNDKEYEDLNGYLENSVWTLHALEPLKVTGLEGSFDLFIRVQGGGKSGQAGAIRLGIARALLQYNPDLRPTLKEKGLLTRDPRMVERKKYGLRKARRAPQFSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICN5</accession>
    <name>B7ICN5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transporter, major facilitator family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101641.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1317"/>
    <dbReference type="EnsemblBacteria" id="ACJ75762">
        <property type="protein sequence ID" value="ACJ75762"/>
        <property type="gene ID" value="THA_1317"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1317"/>
    <dbReference type="eggNOG" id="COG2223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_756328_0_0_0"/>
    <dbReference type="OMA" id="YFYMASF"/>
    <dbReference type="OrthoDB" id="1217595at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="366" mass="40807" checksum="647ABC7BC8B947DA" modified="2009-02-10" version="1">MELLGAIFHFFADFFNAFVKPLAPHFIDKLGIDNKTFASFIALLGGITSIFQVFFGMYFDKIKRDGIYITIIVVLEVFLVSFLGIVKSAILFFSLVVAIRLLNSMFHPLGASFAGRRSKGKHVALFSVAGTLGAGIAPVFITFYQEKIGIEKLYFLTLFFIPFLLLLSFRIFSYEKPVEAKKLSFKGDIFKLWPIFLIVASRSFSMDIFHTYIPIYMNEVGKSLIVGGSILTFGMMFGVFTNFFGTIVLEKTNDKFTNTIGFVGMGIFGLLFVFVPNLWLKVISFVLFDGFSFFTMSSNIVSAQKLLPNNKALASGISMGFSWAIGSFLSSGYAAIFGNNTIFMFLSASVFPLLTILIYNFGVKNN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICR2</accession>
    <name>RPOC_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit beta'</fullName>
            <shortName evidence="1">RNAP subunit beta'</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase subunit beta'</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit beta'</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoC</name>
        <name type="ordered locus">THA_1344</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ICR2"/>
    <dbReference type="SMR" id="B7ICR2"/>
    <dbReference type="STRING" id="484019.THA_1344"/>
    <dbReference type="PRIDE" id="B7ICR2"/>
    <dbReference type="EnsemblBacteria" id="ACJ75789">
        <property type="protein sequence ID" value="ACJ75789"/>
        <property type="gene ID" value="THA_1344"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1344"/>
    <dbReference type="eggNOG" id="COG0086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_3_1_0"/>
    <dbReference type="OMA" id="YRNIRVE"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01322">
        <property type="entry name" value="RNApol_bact_RpoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012754">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="PTHR19376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000165856" description="DNA-directed RNA polymerase subunit beta'">
        <location>
            <begin position="1"/>
            <end position="1649"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="746"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="748"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="750"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1077"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1268"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1275"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1278"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01322"/>
        </source>
    </evidence>
    <sequence length="1649" mass="184820" checksum="58C81D5E75CE8C39" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICV3</accession>
    <name>B7ICV3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin reductase</fullName>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">trxB</name>
        <name evidence="6" type="ordered locus">THA_1385</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-08" db="PDB data bank">
            <title>Structure of thioredoxin reductase from the thermophilic eubacterium Thermosipho africanus TCF52B.</title>
            <authorList>
                <person name="Sahtout N."/>
                <person name="Kuttiyatveetil J.R.A."/>
                <person name="Sanders D.A.R."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS)</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="4">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101768.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="PDB" id="7JYP">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A=1-316"/>
    </dbReference>
    <dbReference type="SMR" id="B7ICV3"/>
    <dbReference type="STRING" id="484019.THA_1385"/>
    <dbReference type="EnsemblBacteria" id="ACJ75830">
        <property type="protein sequence ID" value="ACJ75830"/>
        <property type="gene ID" value="THA_1385"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1385"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_3_0"/>
    <dbReference type="OMA" id="FRAEDYW"/>
    <dbReference type="OrthoDB" id="692968at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="8" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="5">
        <location>
            <begin position="17"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="7JYP"/>
        </source>
    </evidence>
    <sequence length="316" mass="34098" checksum="AA76A735A11279B3" modified="2009-02-10" version="1">MVHFDLGNISTGPKDYYDILIIGGGPAGLTAAIYAGRAGLSAAVFEKALEGGAVTQTHVVENWPGFIRIEGSELGEKFAEHAKAFGAEIITAEVLKISYDNEYKYVELDNGKKVKGKVLIYATGAVPRKLGVPGEEEFRGRGVTYCAACDGYLFSGKDIVVVGGGDSACDEAHFLAKMVKSITMVQNLPYLTAAKVLQDRLLENKNVKVILNSLVKEIRGKDKVEEVVVVNNETGEETVIKAEGVFIYVGLVPKSDLLKGIVDINEYGYIKTDENMETNVPGIYAVGDVREKNLRQIVTAAADGAIAVEHAAKKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID47</accession>
    <name>B7ID47_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, PadR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101963.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1481"/>
    <dbReference type="EnsemblBacteria" id="ACJ75924">
        <property type="protein sequence ID" value="ACJ75924"/>
        <property type="gene ID" value="THA_1481"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1481"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_5_2_0"/>
    <dbReference type="OMA" id="YRHLRQM"/>
    <dbReference type="OrthoDB" id="1706427at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="13"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="113" mass="13067" checksum="7A580A36398EE997" modified="2009-02-10" version="1">MGRFRKTFIEPFVLLIIAQSPLHGYEIANKLLEFGIELTGLGQMGNLYRILSKLEEEGLLRFEWDNTNQGPSKKVYYITPKGLEYLAKSVEELEKINNTISRFIIEAKKLIDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID49</accession>
    <name>B7ID49_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Outer membrane protein CzcC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101967.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1485"/>
    <dbReference type="EnsemblBacteria" id="ACJ75926">
        <property type="protein sequence ID" value="ACJ75926"/>
        <property type="gene ID" value="THA_1485"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1485"/>
    <dbReference type="HOGENOM" id="CLU_2083739_0_0_0"/>
    <dbReference type="OrthoDB" id="2168880at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="117" mass="13574" checksum="0ABD9146260B1761" modified="2009-02-10" version="1">MKKFWGILLFLVLSVTIFSQYYDFEMIVSRSFPKLDRECDESLTEPVTYAICKKWEEKPGYLDNTCLNICQTACQVLPNKIAVLACSVGCYGACWKPESKKCLDWDVIRVYPCNWGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID56</accession>
    <name>B7ID56_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sensory box histidine kinase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1492</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101981.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1492"/>
    <dbReference type="EnsemblBacteria" id="ACJ75933">
        <property type="protein sequence ID" value="ACJ75933"/>
        <property type="gene ID" value="THA_1492"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1492"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038242_0_0_0"/>
    <dbReference type="OMA" id="QIDASYI"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="384"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="503" mass="58915" checksum="4D860128CE8F36FB" modified="2009-02-10" version="1">MFSFIFVLVLINLYNYSLKVEGLKIESFSVVENSKKYGGNLKIPFFFLLKKPSKISLVTKLDNVPKNSFLYIPQIDASYIEIYINDVLIKKIGYKSKIGHFFYSPFLITLPDESRELKIKMSGTYELGIDFPIFIIQENQIPKYWTLKIITFYLVIFLMGTLLSLSIILLLFSINSKRNKKKLFLHFSLASILGSIWLFDTLPLPIFDSLENFTLFKKITLVSLYLSFSFLSKGSFIYFNYNGLSGKILTFLYYLSCIILIFSPSVYHTHIFHNYLSVIFVLNAVFLIYLFFKSNTKKSLILLFILSSFILTAIHDVIVLIFDIETKFLSPLGVLILFFVFSYIIISSYKETVKEKHIYYKKSVFDNLTKAYNRNIFDFEKFHESDLFIYIDINKLKEINDTLGHKYGDKILKKFSQIALNNIRKDDLLIRLGGDEFLIVLKNCSKRKGKLTINRILNEFRNSDETSPTFSWGIIRYKDSLEKTLESGDLLMYKMKNKYKYRN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID58</accession>
    <name>BIOF_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">8-amino-7-oxononanoate synthase</fullName>
            <shortName evidence="1">AONS</shortName>
            <ecNumber evidence="1">2.3.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-keto-8-amino-pelargonic acid synthase</fullName>
            <shortName evidence="1">7-KAP synthase</shortName>
            <shortName evidence="1">KAPA synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">8-amino-7-ketopelargonate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bioF</name>
        <name type="ordered locus">THA_1494</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>6-carboxyhexanoyl-[ACP] + H(+) + L-alanine = (8S)-8-amino-7-oxononanoate + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:42288"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9955"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78846"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="EC" id="2.3.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; biotin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.47" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101993.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ID58"/>
    <dbReference type="SMR" id="B7ID58"/>
    <dbReference type="STRING" id="484019.THA_1494"/>
    <dbReference type="EnsemblBacteria" id="ACJ75935">
        <property type="protein sequence ID" value="ACJ75935"/>
        <property type="gene ID" value="THA_1494"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1494"/>
    <dbReference type="eggNOG" id="COG0156">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_11_0_0"/>
    <dbReference type="OMA" id="MDTHGFG"/>
    <dbReference type="OrthoDB" id="479874at2"/>
    <dbReference type="UniPathway" id="UPA00078"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008710">
        <property type="term" value="F:8-amino-7-oxononanoate synthase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008890">
        <property type="term" value="F:glycine C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010962">
        <property type="entry name" value="AONS_Archaea/Firmicutes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004723">
        <property type="entry name" value="AONS_Archaea/Proteobacteria"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00858">
        <property type="entry name" value="bioF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01825">
        <property type="entry name" value="gly_Cac_T_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0093">Biotin biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000381120" description="8-amino-7-oxononanoate synthase">
        <location>
            <begin position="1"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="108"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P12998"/>
        </source>
    </evidence>
    <sequence length="391" mass="43479" checksum="E03FB70499BC7BC1" modified="2009-02-10" version="1">MFDYSIFSKELENLKEQGLYTYIKTLESPQGAWLTINGKKVLNLCSNNYLGFANEERLKKAAIEAIEKWGVGPGAVRTIAGTFSLHNELEKTLAEFKKVEATIFLQSGFVANQAVIPAITNEEDAILSDELNHASIIDGVRLSKAKRYVWKHRDVKDLEEKLKEAKDARRKLIITDGVFSMDGDLAPLPEIVELAEKYNAMVMVDDAHGEGVLGSHGRGIVDHFGLHGRVDIEIGTLSKAFGVLGGYVAGKKELIDYLKQKARPFLFSSPLSPADTAAALEATKILQESDDRVKRLWDNAKYFKEEMKKLGFDTGESETPITPVMLYDAKLSTNFSKELFEEGIFAQSIGYPTVPKGKARIRVMISAVHTKEDLDFALEKFEKVGKKLGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID68</accession>
    <name>B7ID68_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580260.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1504"/>
    <dbReference type="EnsemblBacteria" id="ACJ75945">
        <property type="protein sequence ID" value="ACJ75945"/>
        <property type="gene ID" value="THA_1504"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1504"/>
    <dbReference type="eggNOG" id="ENOG502ZK55">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_818700_0_0_0"/>
    <dbReference type="OMA" id="FAWIFPW"/>
    <dbReference type="OrthoDB" id="1699509at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="313" mass="35298" checksum="BD69EF92C16C9675" modified="2009-02-10" version="1">MKFFNSYPTFSKLILVLVIGLLSFLIPLWTIKIILFIVGLFIFSTILKKGKIVFFILVFIFSIPFISAKRFSTNFFEFYNFFGNFEVSSNEKYIIQPDKKQFPIYSDITINIKNQNGVIIELVDGDSIEFPSKLNLITDNKKIEINNYGYFSKNYYFVIKIGTKDLKNLKISATKIEINGKAKLDNLILNGTAINMKNCSIESEYLHVSGTAINLSDFFIGNYAYIDGTALNFSGTFDFQQIKIDGTSININLNIGKLNKETIIDGTAINATINFLSDNPKILNIDGTSGTVKVKNYESATINVDGPKIILEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID72</accession>
    <name>B7ID72_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Geranylgeranyl diphosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1508</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102029.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1508"/>
    <dbReference type="EnsemblBacteria" id="ACJ75949">
        <property type="protein sequence ID" value="ACJ75949"/>
        <property type="gene ID" value="THA_1508"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1508"/>
    <dbReference type="eggNOG" id="COG0142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_2_1_0"/>
    <dbReference type="OMA" id="LELFQNW"/>
    <dbReference type="OrthoDB" id="1861068at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="338" mass="39331" checksum="B4B4AA22FE0309F3" modified="2009-02-10" version="1">MDFNEFKKMHQKKVEEYIEKVILENKVNPPKQIESLIRTLIEYCLRPGKRIRPLLFLLGATSYAKKELDYEKLYRIAAVIEIMHCFLLIHDDIMDRSALRRGLPSLHVVFYEEFKEKNNNEKIGEDLALVFGDILFFIAIRQLSKLNLSSKFLEEFSNCYINTGYGQTLDVLYSLGKYKPDEDKISVEIARLKTAYYTFFYPFYLGVLLSNENIDKKKIEDAIIPAGIAFQIRDDIISTFDEKSGKSNVTDILEGKLTALIDFGEYDEKFFEVYSKQEKSSEEIQYILDQILKSGAIKKAKNAMYTLFEESISKIRKLDLNDTSKDILIDLISRLRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID84</accession>
    <name>B7ID84_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable cell division protein WhiA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">whiA</name>
        <name evidence="8" type="ordered locus">THA_1520</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in cell division and chromosome segregation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WhiA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102049.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1520"/>
    <dbReference type="EnsemblBacteria" id="ACJ75961">
        <property type="protein sequence ID" value="ACJ75961"/>
        <property type="gene ID" value="THA_1520"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1520"/>
    <dbReference type="eggNOG" id="COG1481">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053282_0_1_0"/>
    <dbReference type="OMA" id="RVQIFIP"/>
    <dbReference type="OrthoDB" id="1634356at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043937">
        <property type="term" value="P:regulation of sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01420">
        <property type="entry name" value="HTH_type_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018478">
        <property type="entry name" value="Sporu_reg_WhiA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003802">
        <property type="entry name" value="Sporulation_regulator_WhiA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023054">
        <property type="entry name" value="Sporulation_regulator_WhiA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039518">
        <property type="entry name" value="WhiA_LAGLIDADG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37307">
        <property type="entry name" value="PTHR37307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02650">
        <property type="entry name" value="HTH_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14527">
        <property type="entry name" value="LAGLIDADG_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10298">
        <property type="entry name" value="WhiA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00647">
        <property type="entry name" value="DNA_bind_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 4" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WhiA_N" evidence="6">
        <location>
            <begin position="23"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="LAGLIDADG_WhiA" evidence="7">
        <location>
            <begin position="117"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="HTH_WhiA" evidence="5">
        <location>
            <begin position="214"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="305" mass="35203" checksum="4D0AA33EA338856E" modified="2009-02-10" version="1">MLYTFSEDVKGELCHTVVNSELEAKSELAGFLKSRGVIIRSFNDVVVKIEVGFIPAARRIMNLMAIVGTDKKRLTMIKNKLKKKRVQIFIPFSILSKLELDILEIPAYIFEDLGLFGAFLRGVFISTGSVTDPTKNYHFEIISHNYKFLNEIQKYLFDNLGILGKIASFGEKNYRFYLKRAKDIIEILYYMGAQRNADKMEKIVSSREIKSDFNRSMNFLTANAKRVGESNAKQIRIIKNLFEKYGEDILNGELKKIALLRLENEDLSLSDLGKLFDPPLSKSMVYNRIKKIFKIYEELESKNME</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID91</accession>
    <name>B7ID91_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_1527</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102057.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1527"/>
    <dbReference type="EnsemblBacteria" id="ACJ75968">
        <property type="protein sequence ID" value="ACJ75968"/>
        <property type="gene ID" value="THA_1527"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1527"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_0"/>
    <dbReference type="OMA" id="VMVPQDG"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="19"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="340"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="373"/>
            <end position="380"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="578" mass="66765" checksum="72B0FE81B52C57BE" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID94</accession>
    <name>B7ID94_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide transport ATP-binding protein AppF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1530</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102060.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1530"/>
    <dbReference type="EnsemblBacteria" id="ACJ75971">
        <property type="protein sequence ID" value="ACJ75971"/>
        <property type="gene ID" value="THA_1530"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1530"/>
    <dbReference type="eggNOG" id="COG4608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_0"/>
    <dbReference type="OMA" id="VFYKGQD"/>
    <dbReference type="OrthoDB" id="1303270at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="7"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="51"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="331" mass="37769" checksum="25FCB21ABF7E47D6" modified="2009-02-10" version="1">MNNETIIKVQNLKKYFPITKGFLVKKHVGDVKAVDDISFEVKKKETFALVGESGCGKTTAARTMLRLIDPTDGKIEIFGQDISNLNRKELLPFRRKMQIVFQNPIGSLNPRMTIGQILTEPLLFHKIVKDKKEAYDKAVEILRMVGLKPYHMDRYPHQFSGGQKQRIAIARALSVGPEIIFLDEPTSALDVSVQAQIINLFLKFQEELDLTYIFISHDLSLVRFISDKVAVMYLGRIVEMGDVDEVFENPIHPYTKALLSASPIPDPKVEKQRKRIILTGNVPNPIARPSGCFFHPRCPFKMEKCEKEYPQMYKISENHQVSCHLVEKEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDA9</accession>
    <name>B7IDA9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Adenylosuccinate lyase</fullName>
            <shortName evidence="10">ASL</shortName>
            <ecNumber evidence="4 10">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">purB</name>
        <name evidence="12" type="ordered locus">THA_1547</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:23921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = AMP + fumarate</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:16854"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="4 10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580271.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1547"/>
    <dbReference type="EnsemblBacteria" id="ACJ75986">
        <property type="protein sequence ID" value="ACJ75986"/>
        <property type="gene ID" value="THA_1547"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1547"/>
    <dbReference type="eggNOG" id="COG0015">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030949_0_1_0"/>
    <dbReference type="OMA" id="ASSCEKI"/>
    <dbReference type="OrthoDB" id="347727at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADSL_C" evidence="11">
        <location>
            <begin position="349"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ75986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="425" mass="49354" checksum="177ACC99EC4E423D" modified="2009-02-10" version="1">MVERYALEPLKSHWTLESQYKRWLEVELAVIRAYEELKVAPKGTYERAKKNSYLNVEEILETEKVVDHDVIAFIKVTTSKMGDESRYFHFGLTSSDVVDTANSLALLRATRYIYAELENLHEVLLKKAFEYKKLPTIGRTHGVHAEPTSFGLKYLSWAAEIERNIERLKNAMDEISVGKLSGAVGNYANIDPEVEKLALSYLSLKPVKVATQVIPRDIHANLINVFALIASAIERIAVEIRHLQKTETLEVQEPFKKGQRGSSAMPHKKNPILCERLTGMSRVIRSYVNSALENIVLWHERDISHSSVERYMFPDITMTLFYMIKKATYLIENLTVFEDNVVKNIDKTKGLVYSQRVLLKLVEKGYTREEAYKEVQKIALKCWENKSSFKNEVKNYFKFSQEEIDEIFNPEYYLRNIDVIYERFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDG4</accession>
    <name>B7IDG4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TrkA-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1603</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102358.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1603"/>
    <dbReference type="EnsemblBacteria" id="ACJ76041">
        <property type="protein sequence ID" value="ACJ76041"/>
        <property type="gene ID" value="THA_1603"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1603"/>
    <dbReference type="eggNOG" id="COG0569">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046525_4_1_0"/>
    <dbReference type="OMA" id="NEYAFHR"/>
    <dbReference type="OrthoDB" id="1768241at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00335">
        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0630">Potassium</keyword>
    <keyword evidence="1" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="3">
        <location>
            <begin position="8"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="141" mass="15534" checksum="A496A1E7A0398B8F" modified="2009-02-10" version="1">MRKTNELFIVIIGCGKVGGNIASMASSIGHSVVVIDKNQESFENLSPEFTGFTIIGDATEKDVLENAKISKADYVFVLTEDDNTNFLISLQCKYYFGAKNIIARVYDPENSVLFLEYNINVVSPTMLLIGDLKNILVGESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDN6</accession>
    <name>B7IDN6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipopolysaccharide biosynthesis protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1676</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102496.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1676"/>
    <dbReference type="CAZy" id="GT26">
        <property type="family name" value="Glycosyltransferase Family 26"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76113">
        <property type="protein sequence ID" value="ACJ76113"/>
        <property type="gene ID" value="THA_1676"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1676"/>
    <dbReference type="eggNOG" id="COG1922">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063203_3_1_0"/>
    <dbReference type="OMA" id="PWRWRRM"/>
    <dbReference type="OrthoDB" id="1759731at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06533">
        <property type="entry name" value="Glyco_transf_WecG_TagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004629">
        <property type="entry name" value="WecG_TagA_CpsF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34136">
        <property type="entry name" value="PTHR34136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03808">
        <property type="entry name" value="Glyco_tran_WecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00696">
        <property type="entry name" value="wecG_tagA_cpsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="238" mass="27599" checksum="DB35CD18ABD10567" modified="2009-02-10" version="1">MDIVNFSQLKITVGFENEIREEIVKSIISNEKTFIVTLNASILLRALKDGYYRNVVNNATYIIPDGSGIVWALKRNRNILTDRITGIDTMLYLCEKSKEYNWKVYLLGAKPKVIEEAAKKLKNNGVNVVGFHHGYFPFDDKSVSEEIERLKPDLVFVGMGVPRQEEWIFNNISLPFKFAMGVGGSFDVISGLKKRAPLIFQKMKLEWFYRWLQSPIKKRHVPFEIIKYTYLVLRGKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDP7</accession>
    <name>B7IDP7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Membrane bound hydrogenase, MbxM subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1687</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580349.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1687"/>
    <dbReference type="EnsemblBacteria" id="ACJ76124">
        <property type="protein sequence ID" value="ACJ76124"/>
        <property type="gene ID" value="THA_1687"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1687"/>
    <dbReference type="eggNOG" id="COG0650">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_0_2_0"/>
    <dbReference type="OMA" id="PWYQNFI"/>
    <dbReference type="OrthoDB" id="1559067at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="295" mass="32762" checksum="D7EBA02F1B6199E9" modified="2009-02-10" version="1">MINTILKVMAVLFTGFFVGLTLEGVARKVIARIQRRYGPPWYQNFIDLFKTFSKHGITHGWIFDFGVLMALGGTIATLAFVPLGNLIAFPGLDNVFIILYLWAIGALGMAMGMVGSGNPNASIGVARALTQMMGYEVPYLVVLAGVMFYYKTSSVSALMYSQADKWTLLKMPIGGIIAFVSLMGMLGKKPFDTPIAPAEIASGPMVELSAKYMGLLMLMHTFSIFVEISMFVNIFLGISNYFTFLIKFTIVWILATMISAVLPRFRIEQVVKFYWKVPLSLAFVQVLFVLLGWVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDR8</accession>
    <name>B7IDR8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription termination factor Rho</fullName>
            <ecNumber evidence="8">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ATP-dependent helicase Rho</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rho</name>
        <name evidence="11" type="ordered locus">THA_1709</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. The homohexamer assembles into an open ring structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the Rho family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1709"/>
    <dbReference type="EnsemblBacteria" id="ACJ76145">
        <property type="protein sequence ID" value="ACJ76145"/>
        <property type="gene ID" value="THA_1709"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1709"/>
    <dbReference type="eggNOG" id="COG1158">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016377_4_3_0"/>
    <dbReference type="OMA" id="TRLCRAH"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008186">
        <property type="term" value="F:ATP-dependent activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04459">
        <property type="entry name" value="Rho_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01884">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011112">
        <property type="entry name" value="Rho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036269">
        <property type="entry name" value="Rho_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011113">
        <property type="entry name" value="Rho_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004665">
        <property type="entry name" value="Term_rho"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46425">
        <property type="entry name" value="PTHR46425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07498">
        <property type="entry name" value="Rho_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07497">
        <property type="entry name" value="Rho_RNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00959">
        <property type="entry name" value="Rho_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68912">
        <property type="entry name" value="SSF68912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00767">
        <property type="entry name" value="rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51856">
        <property type="entry name" value="RHO_RNA_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 8" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="Rho RNA-BD" evidence="10">
        <location>
            <begin position="54"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA-binding 1" evidence="8">
        <location>
            <begin position="67"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA-binding 1" evidence="8">
        <location>
            <begin position="83"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA-binding 1" evidence="8">
        <location>
            <begin position="113"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA-binding 2" evidence="8">
        <location>
            <begin position="286"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="171"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="183"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="RNA-binding 2" evidence="8">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ76145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="421" mass="47587" checksum="C9EDE9BDCE8A60E7" modified="2009-02-10" version="1">MSNNDAYNIKDLESMTIRDLYKLAKEYDIPRYTSLKKQDLIFAILEARAQSEGYFFGEGVLEVHPDGFGFLRSLESMLPSSKDIYISNSQIRKFNLNTGDIISGVIRPPKEGERFLPMIKIEAVNYKDPEFANERVNFENLTACYPDKQFFLETNKDTYSTRLIDLFSPIGKGQRGMIVAPPKAGKTTILKEIANGIAKNHPDTIRIVLLIDERPEEVTDIKESVNAWVIAAPFDMPPDKQIKKAELTLEMAKRLVEFGYDVVILMDSLTRLARVYNINVPPSGKLLSGGVDPAALYKPKHFFGAARNTKEGGSLTIIATSLIETGSKMDEVIFEEFKGTGNMELVLSRQLANKRIFPAINIMLSGTRREELLLSNEKLKKLWLLRKMLSSMSEEEGLKLILNKMKETNTNEDFLALIDLQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDT4</accession>
    <name>APGM_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <shortName evidence="1">BPG-independent PGAM</shortName>
            <shortName evidence="1">Phosphoglyceromutase</shortName>
            <shortName evidence="1">aPGAM</shortName>
            <ecNumber evidence="1">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">apgM</name>
        <name type="ordered locus">THA_1725</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580374.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDT4"/>
    <dbReference type="SMR" id="B7IDT4"/>
    <dbReference type="STRING" id="484019.THA_1725"/>
    <dbReference type="PRIDE" id="B7IDT4"/>
    <dbReference type="EnsemblBacteria" id="ACJ76161">
        <property type="protein sequence ID" value="ACJ76161"/>
        <property type="gene ID" value="THA_1725"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1725"/>
    <dbReference type="eggNOG" id="COG3635">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034906_2_0_0"/>
    <dbReference type="OMA" id="IAFRCNF"/>
    <dbReference type="OrthoDB" id="1293048at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046537">
        <property type="term" value="F:2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01402_B">
        <property type="entry name" value="ApgM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023665">
        <property type="entry name" value="ApgAM_prokaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="PTHR31209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000145451" description="Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase">
        <location>
            <begin position="1"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01402"/>
        </source>
    </evidence>
    <sequence length="402" mass="44498" checksum="A1C9F344008E31C0" modified="2009-02-10" version="1">MIDRQKVISELISPNTSKIVLLVMDGIGDIPNEEGLTPLQKANTPNLDALAKKSDLGQTIPVLPGITPGSGPGHLGLFGYDPLKYQIGRGILEALGINVEVGENDLVARGNFATIDGDIIVDRRAGRPSSEESAKVVEILNENIKEIEDVKITFYPGKEHRFVVKFTGEGLMDKLEDADPQKEGKPIKYTKALDESSKKSERIVNILLDKIKEVLKDQPKMNFALLRGFSKHPDMPKFGDVFKLKPAAVAVYPMYKGLAKLVGMEVVEAGQTIEDEFNTVKKLWNEYDFFYVHIKKTDSYGEDGNFDSKVKVIEEVDKFLPILLELNPDVLIVTGDHSTPCVMKGHSFHPVPLMIYAKNTRRGLSKLFNEFECARGSLGTIHAVDVMPLALAYAGRLEKYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDV0</accession>
    <name>B7IDV0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1743</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580382.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1743"/>
    <dbReference type="EnsemblBacteria" id="ACJ76177">
        <property type="protein sequence ID" value="ACJ76177"/>
        <property type="gene ID" value="THA_1743"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1743"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_709664_0_0_0"/>
    <dbReference type="OMA" id="WIVRFRV"/>
    <dbReference type="OrthoDB" id="821953at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="389" mass="45566" checksum="FDFD74803A3AA33C" modified="2009-02-10" version="1">MKRVLILAFSFVSYILFSYTIGVITSYSTETYSLLVDASNLINEEYSEYKDYVKFINSSSDLTCDYYVNLYLTYDSTNNLYKATYKDFDYIVSSVYSPKGYKKYSTFLEEIVYYPLEKISINKLKRRDFDNYLRLTFYPGVDEYGDFKDGKFLFITDRLGGNRNLAYIDIQNEKITLLPVWSSSEYYPRFSPDMKMLLFQGSLHGNWSIYTMPFGVSDYSKKIKKISDSNLPSYTPVWYDNDNVLYIQDTEKGNVMIMANIYNGKKQVLNVYGDMVFTPFVYDGNIYYTSLNGANFGIYKYVDGKNFKVEDSFYNEHDPVIYKNKLVFTSNRDGIYRIWMKDLDTSSVTCLTSDINYDVFYPTVSDGLLFFSVYKDGEEPDIFVKKLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDV6</accession>
    <name>B7IDV6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable chemoreceptor glutamine deamidase CheD</fullName>
            <ecNumber evidence="3">3.5.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cheD</name>
        <name evidence="4" type="ordered locus">THA_1749</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Probably deamidates glutamine residues to glutamate on methyl-accepting chemotaxis receptors (MCPs), playing an important role in chemotaxis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>H2O + L-glutaminyl-[protein] = L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16441"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="EC" id="3.5.1.44"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CheD family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.44" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580386.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1749"/>
    <dbReference type="EnsemblBacteria" id="ACJ76183">
        <property type="protein sequence ID" value="ACJ76183"/>
        <property type="gene ID" value="THA_1749"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1749"/>
    <dbReference type="eggNOG" id="COG1871">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087854_2_0_0"/>
    <dbReference type="OMA" id="RIGGMCH"/>
    <dbReference type="OrthoDB" id="1863047at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050568">
        <property type="term" value="F:protein-glutamine glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16352">
        <property type="entry name" value="CheD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01440">
        <property type="entry name" value="CheD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038592">
        <property type="entry name" value="CheD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005659">
        <property type="entry name" value="Chemorcpt_Glu_NH3ase_CheD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011324">
        <property type="entry name" value="Cytotoxic_necrot_fac-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35147">
        <property type="entry name" value="PTHR35147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03975">
        <property type="entry name" value="CheD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64438">
        <property type="entry name" value="SSF64438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="160" mass="17328" checksum="DD662558481EF990" modified="2009-02-10" version="1">MPIKSKKIIGIGDYAIGDKNTILVTLGLGSCVGVCIRDKNKLIGAMVHVMLPDSDGKEINKPGKYADTGIKIIVEELKKLGATTFEAKIAGGASMFEKSAMDVGKRNVESVKKWLSKFGIRIVSEDTGGNRARSIEYDIETGKLMVRKVKTGENVEVIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDZ3</accession>
    <name>B7IDZ3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA threonylcarbamoyladenosine biosynthesis protein TsaE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1789</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TsaE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1789"/>
    <dbReference type="EnsemblBacteria" id="ACJ76220">
        <property type="protein sequence ID" value="ACJ76220"/>
        <property type="gene ID" value="THA_1789"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1789"/>
    <dbReference type="eggNOG" id="COG0802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087829_5_0_0"/>
    <dbReference type="OMA" id="RIWILEG"/>
    <dbReference type="OrthoDB" id="1827295at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003442">
        <property type="entry name" value="T6A_TsaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02367">
        <property type="entry name" value="TsaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00150">
        <property type="entry name" value="T6A_YjeE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="172" mass="20375" checksum="30F5D55A231169F5" modified="2009-02-10" version="1">MFEALELNDLKKLSQIISKFYIKYPVRFLYLNGDLGAGKTTFSKFFCENFGVDPDLVSSPTFSIVNVYEGYKTIYHVDLYRLESPDELFYVLEENFEDEDGIFLIEWSNLFENYFTEKGITLNFFHRNDGKRNVEIIIDTKLSSLDLIEDLRRWQDDREKVRKIGKESSKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDZ6</accession>
    <name>B7IDZ6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">3-oxoacyl-[acyl-carrier-protein] synthase 2</fullName>
            <ecNumber evidence="3 10">2.3.1.179</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">fabF</name>
        <name evidence="14" type="ordered locus">THA_1792</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor. Can efficiently catalyze the conversion of palmitoleoyl-ACP (cis-hexadec-9-enoyl-ACP) to cis-vaccenoyl-ACP (cis-octadec-11-enoyl-ACP), an essential step in the thermal regulation of fatty acid composition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(9Z)-hexadecenoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxo-(11Z)-octadecenoyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:55040"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10800"/>
            <dbReference type="Rhea" id="RHEA-COMP:14074"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:83989"/>
            <dbReference type="ChEBI" id="CHEBI:138538"/>
            <dbReference type="EC" id="2.3.1.179"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a fatty acyl-[ACP] + H(+) + malonyl-[ACP] = a 3-oxoacyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 12">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.179" evidence="3 10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580417.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1792"/>
    <dbReference type="EnsemblBacteria" id="ACJ76223">
        <property type="protein sequence ID" value="ACJ76223"/>
        <property type="gene ID" value="THA_1792"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1792"/>
    <dbReference type="eggNOG" id="COG0304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_69_2_0"/>
    <dbReference type="OMA" id="QIGHCLG"/>
    <dbReference type="OrthoDB" id="606297at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="PTHR11712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="B_KETOACYL_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_KS" evidence="13">
        <location>
            <begin position="4"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ76223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="408" mass="43817" checksum="929381479A0FEE7F" modified="2009-02-10" version="1">MKRVVITGMGTINPIGKNISEFSENLKKMHIGIDKISSFNAENLPVKIAGEVTGFVPEEYIDKKLSKRIDRYTQFALVAAKEAIENSKINFEGIEERVAVIVASGMGGFITLDKQNDVFKERGANRVSPFMIPMILSNMASGVVAMNYKLKGPNFSVVSACASSVHAIALGALLIRHGYVDVAVVGGAEATIAPLPIAGFAAMKALSTRNDEPKKASRPFDVERDGFVMAEGAGILILESEEFAKKRNANILAQVKGFGMNDDAYHFSAPDPQSKGSTSVMLQSLKDANLSPDEIDLVSCHATSTPVGDELEAQAILNVFGERNNLFVNSTKALTGHLLGAAGVVETIAAILEMNEGFVHGMPNLEKPDEIASKLNLVKETREAKINNFLKNSFGFGGHNASVVIGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE15</accession>
    <name>B7IE15_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ribonuclease III</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1811</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1811"/>
    <dbReference type="EnsemblBacteria" id="ACJ76242">
        <property type="protein sequence ID" value="ACJ76242"/>
        <property type="gene ID" value="THA_1811"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1811"/>
    <dbReference type="eggNOG" id="COG0571">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000907_1_3_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="SSF69065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="5">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="4">
        <location>
            <begin position="90"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="159" mass="18344" checksum="75C02378957E7334" modified="2009-02-10" version="1">MAQIKAAVASEDILYEIGRDIGLENYIFLGKGELKSGGKYKKSIIADVVEALVAAIYVDCKKDLNVLENVFHDTFKRYIEIFFSGKRIFDYKTKLQEITQDRFKQLPVYDTVSQKNKFITTLYIDGKIYSKAEGFSKKESEKLAAKIAYEKLKEEEFHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE37</accession>
    <name>B7IE37_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HD-GYP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1833</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1833"/>
    <dbReference type="EnsemblBacteria" id="ACJ76264">
        <property type="protein sequence ID" value="ACJ76264"/>
        <property type="gene ID" value="THA_1833"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1833"/>
    <dbReference type="eggNOG" id="COG3437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_541696_0_0_0"/>
    <dbReference type="OMA" id="NNERINT"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="HD-GYP" evidence="4">
        <location>
            <begin position="281"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="3">
        <location>
            <begin position="303"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="472" mass="54475" checksum="E266FA9AC162DE02" modified="2009-02-10" version="1">MRKNNVIRFNLAFFTTLIIIITILIHGFLLKLNIRDSSRFLANYFDNLISSRVFLKSKLDRNFFDPQMKIDLSKILVEGPNDYIKGIYYSSNSIKYVEKSRDNYLLYEIPLTLFNFNQSSLKHYEILVNNKVVYSNEISKIGSEYHSNILDSKYLSKYGFEIVVGYKTSFFIGSYLLIVLPILVLLLIFYYILYNLRKDAVGLDITLKNLTIELKKTFEEFKTKRVLSFNKQITNNERINTLQEIFISMFNEFKKIMNEHEKTNEILEETMAELEEANAELVEKNLQIISALAEAVEIKDSVTGNHSKNVMNLSIELAKELDVSDPAELEAIKYGAILHDIGKIGIPEHILNKPGKLDDKEFEIMKKHTIFGEKIIKTIPGWDLVADVIRHHHENWDGSGYPDGLKDGEISLRAQIVAIVDVFVALTEDRPYRKGLSVEKAIEIMKEMVGSKFSPELFEKFVIVLKKRGLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE89</accession>
    <name>B7IE89_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3D domain family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1890</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102895.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1890"/>
    <dbReference type="EnsemblBacteria" id="ACJ76316">
        <property type="protein sequence ID" value="ACJ76316"/>
        <property type="gene ID" value="THA_1890"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1890"/>
    <dbReference type="eggNOG" id="COG3584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4942">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024923_0_0_0"/>
    <dbReference type="OrthoDB" id="1081105at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009254">
        <property type="term" value="P:peptidoglycan turnover"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010611">
        <property type="entry name" value="3D_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06725">
        <property type="entry name" value="3D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="SSF50685"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="3">
        <location>
            <begin position="263"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="203"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="531" mass="61118" checksum="402635D6E7317EC8" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEA7</accession>
    <name>B7IEA7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arsenic transporter family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1909</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580477.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1909"/>
    <dbReference type="EnsemblBacteria" id="ACJ76334">
        <property type="protein sequence ID" value="ACJ76334"/>
        <property type="gene ID" value="THA_1909"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1909"/>
    <dbReference type="eggNOG" id="COG1055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011920_4_0_0"/>
    <dbReference type="OMA" id="IAMFIDV"/>
    <dbReference type="OrthoDB" id="626718at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="CitMHS" evidence="7">
        <location>
            <begin position="16"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="422" mass="47106" checksum="F172810E5C54FB66" modified="2009-02-10" version="1">MTIDAIISLFIFVVVYYLIISEKVHRSISVVLGAFILTFFGVFEDPDYLFKNYVDFDTIFLLIGMMLLVSAVKSTGFFEYVAFKLIHFSKNSFLSIFILLNFFVAFFSAFVDNVTTIMIFIPITLAVADAAGIDPTFFVLSEVFSSNIGGTATLIGDPPNILIGNAARLTFNDFILNTSPATLITWGTIILYFVLKNKKYLKKNISFEFSAIEITKSNLIKSTVLLISVIILFTIQEKIGVHSSVIAFGMGFLSILVIDPKNVEKHFGEIEWGTIFFFIGLFIITGALEDTGILKNLAMILSKNFGSTPKLFGMFLIIMSFVVSGFFDNIPFTATMIPVIKMLPSINSTFVNLNPYWWALSLGVCFGGNLTQIGASANIVAITMLLKYSKRSVSFKEYMKFSLIPSLISLIISIAYVEFRYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEB0</accession>
    <name>B7IEB0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 14">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="7">IMP dehydrogenase</shortName>
            <shortName evidence="7">IMPD</shortName>
            <shortName evidence="7">IMPDH</shortName>
            <ecNumber evidence="7 14">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 16" type="primary">guaB</name>
        <name evidence="16" type="ordered locus">THA_1912</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 14">
            <text>H2O + IMP + NAD(+) = H(+) + NADH + XMP</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="7">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 14">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 13">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="7 14"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580478.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1912"/>
    <dbReference type="EnsemblBacteria" id="ACJ76337">
        <property type="protein sequence ID" value="ACJ76337"/>
        <property type="gene ID" value="THA_1912"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1912"/>
    <dbReference type="eggNOG" id="COG0516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022552_2_1_0"/>
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        <location>
            <begin position="244"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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        <location>
            <position position="244"/>
        </location>
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            <name>NAD(+)</name>
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    </feature>
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        <location>
            <begin position="294"/>
            <end position="296"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
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        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>K(+)</name>
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        </ligand>
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        <location>
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        </location>
        <ligand>
            <name>K(+)</name>
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        </location>
        <ligand>
            <name>K(+)</name>
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        </ligand>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000130-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ACJ76337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="483" mass="52208" checksum="64FC6B1B20BFC04A" modified="2009-02-10" version="1">MREALTFDDVLLVPGYSEVLPAQVDVTTRLTRKIELKIPLLSAAMDTVTEAELAKAIAREGGIGIIHKNMTIEEQAHQVKIVKRTENGIIDDPVTILPNVTVEEADKIMAEYKIGGLPVVDENNKLLGLITNRDIRFERNPKRQVSELMTPKDKLVIAKKGISIEEARDILHENKIEKLPLINEDGTLAGLITIKDIKSVVEHPNASRDSKGRLLVGAAVGTSEDTLIRVEALVKAGVDVIVIDTAHGHSKKVIETLKMVKREFPDLQVIAGNVATAQATEELIKNGADAVKVGIGPGSICTTRVVAGIGVPQLTAIMDCVEVAKKYDVPIIADGGIRFSGDIVKALAAGAETVMLGSIFAGTEEAPGETILYQGRKYKSYRGMGSLGAMSRGSADRYFQSGNQKFIPEGVEGMVPFKGNVKDVVYQLIGGLRSGMGYVGAANIKELQQKAQFIKITPASVKESHPHDIIVTKEPPNYWSKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEB5</accession>
    <name>B7IEB5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Arsenic transporter family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">THA_1918</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CitM (TC 2.A.11) transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1918"/>
    <dbReference type="EnsemblBacteria" id="ACJ76342">
        <property type="protein sequence ID" value="ACJ76342"/>
        <property type="gene ID" value="THA_1918"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1918"/>
    <dbReference type="eggNOG" id="COG1055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011920_4_0_0"/>
    <dbReference type="OMA" id="YFMYRNK"/>
    <dbReference type="OrthoDB" id="626718at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015105">
        <property type="term" value="F:arsenite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000802">
        <property type="entry name" value="Arsenical_pump_ArsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00758">
        <property type="entry name" value="ARSENICPUMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="225"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
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            <end position="294"/>
        </location>
    </feature>
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        <location>
            <begin position="314"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="357"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="CitMHS" evidence="10">
        <location>
            <begin position="15"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ76342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="425" mass="46701" checksum="36FFAE32088B4B70" modified="2009-02-10" version="1">MIPKIISLVIFFLTYYLIIFGKGNKAVIAFSMGLLISLVKVSDTLRVSNVGSFIDFNTLGILLGMMIIVGILKTTGLFQAIAIYIVKKSNGNLIYIFVSTLIAVAILSSVLDNVTTLLLFSPIIIYICQEIEIKPETFLFPMIFAANIGGTATMIGDPPNILVGSASGTSFLKFLLVMIVPSLISLFLTILYFLYTLKELKLVKKDKLNIVMESDPKKAIVDYNLLKKGLLIFVLVIVGFFIHEYLDYEAALIALTGSAVMLMISKKDFDEISSEIEWDTLFFFVGLFAIVKALEDVHVIQDITNLIFNFTSHPYILILIVLWGSGILAAFMGAVPVVTIFIPIAKALVGTFPNSDLLWWALALGASFGGNGTISGAASNMVIVGMIENNFNRKIKFAKFMKLGMKIATLGLLVSSLYLYLLLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEC9</accession>
    <name>B7IEC9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1933</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102986.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1933"/>
    <dbReference type="EnsemblBacteria" id="ACJ76356">
        <property type="protein sequence ID" value="ACJ76356"/>
        <property type="gene ID" value="THA_1933"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1933"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_1_0"/>
    <dbReference type="OMA" id="NTYLMYG"/>
    <dbReference type="OrthoDB" id="1074175at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="84"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="290" mass="32414" checksum="3B95E4157A89D22A" modified="2009-02-10" version="1">MAGFTPKTNPKRFHKSQIKFYFILIPIAAFMLLPIIFIFSQAFKPIDELFLYPPRFFVRKPTLNNFYELFAATKGSTIPVSRYLLNSILTALFTVIFTIIISVFAGYALSKKRFKAKNLIFTINNLALMFVPVAVIIPRYLIIQKLGLIDTFVINILSLLAMPIGIFLIKQFVDQIPDALIEAALIDGANDFQIIFNLIMPLLKPAISTVGILAFQVAWNSADASTYFINNENLRTFAFYVSNLTAVTGNTIAGQGIAAAASLIMFVPNLVLFIFMQSRVMNTMAHSGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEE0</accession>
    <name>B7IEE0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TGc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103022.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1945"/>
    <dbReference type="EnsemblBacteria" id="ACJ76367">
        <property type="protein sequence ID" value="ACJ76367"/>
        <property type="gene ID" value="THA_1945"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1945"/>
    <dbReference type="eggNOG" id="COG1305">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044992_0_0_0"/>
    <dbReference type="OMA" id="NLKGDCG"/>
    <dbReference type="OrthoDB" id="575975at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGc" evidence="1">
        <location>
            <begin position="303"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="439" mass="51993" checksum="FEAD48A4C57AF9C0" modified="2009-02-10" version="1">MKFLTTPIPKDIEKLINLGMFKQVKENILKRFKKPIPESLKERLKFELFRLELLEKAYPYNEKEAFELFKKLFKNAKKQEFEKLLEDGYIDFRFINGEKRFQERFHYNVGFTLKEYKERQKKDKSRRNLRKLTNDTIVRLLKTKQEKTYSVSAKISIKRKNPKNEDVCVWLPVPFEKFQQTSVKITNASHDYILSSPTTLQKTVHMKGKDTETFWVEFSYNISEWIGENSKYKYKPTKADLSEKPPHVLFTPYLKEVLQKILKAVKPNNDYEIAKSIYDYLTKNVYYSYVLPYALYDNIPEYVTTVFRGDCGFYAVTFITLCRLAGIPAKWQSGWFATPSGASPHDWALVYLKDFGWVPADLSFGGSRRDNEEMRMFYFGNLDGFRMFANLDFQADFSPKKKYFRNDPYDNQVGEMESKDGYIIDTESEIEILSFKEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEH0</accession>
    <name>B7IEH0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1976</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580521.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1976"/>
    <dbReference type="EnsemblBacteria" id="ACJ76397">
        <property type="protein sequence ID" value="ACJ76397"/>
        <property type="gene ID" value="THA_1976"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1976"/>
    <dbReference type="eggNOG" id="COG0300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_1_0"/>
    <dbReference type="OMA" id="MGTDNIP"/>
    <dbReference type="OrthoDB" id="1186478at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="254" mass="28737" checksum="DCD731B6B025F395" modified="2009-02-10" version="1">MPRFQKGEWVLITGGSSGIGEEFAYKIAEMGLNLVLVGRSEQRLESVKRKIENVNKNIRVETLSFDLSNDIEDLIKKLDNYPIDHLINNAGFGWYGEFVNGNKEIYKNMISVNIKALTILSYHFSKKFIEKGKGGIINVASVAGFFPIPHFAVYGATKAYVYSLSYALWAELKKHNVHVMCLAPGKTKTRFFERANMQNSDKTMSAKKVVEGALKAYSKNKPLYIPGFSNKLSYLLVRKIFSDKSISKLLEKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEI0</accession>
    <name>B7IEI0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar transport membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1986</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103059.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1986"/>
    <dbReference type="EnsemblBacteria" id="ACJ76407">
        <property type="protein sequence ID" value="ACJ76407"/>
        <property type="gene ID" value="THA_1986"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1986"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_3_0"/>
    <dbReference type="OMA" id="TKPWRNG"/>
    <dbReference type="OrthoDB" id="1619224at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="63"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="289" mass="32548" checksum="B3E879664E94D1E2" modified="2009-02-10" version="1">MTKTFSKKWIPYFLIAPTLIYYIIFWLRPVVSAIYYSFFDDNNVFTINNYLTIFRDEYFKKAVINTSIFVVISVTLEFVVALGLALIINKKFKGAQVLLSIALIPMALPAVAVGAMWSSGFATYGWVNSLLYHLGLISADGKIAFLAGNDFQSLMLIILIDAWQVIPFMMVILLAGLQGLSKEAIEAGYIFGGSRFTVLRKITLPMLKPSIQTALILRIISAIQVWLIIVMIYGYRRIPVLLEEVVFYEEQLLGFYRVALAYSVIVAAIVSVVSIIYLKVSGAFEREEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEK1</accession>
    <name>B7IEK1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_7</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_7"/>
    <dbReference type="EnsemblBacteria" id="ACJ74515">
        <property type="protein sequence ID" value="ACJ74515"/>
        <property type="gene ID" value="THA_7"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_7"/>
    <dbReference type="eggNOG" id="COG1403">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_454864_0_0_0"/>
    <dbReference type="OrthoDB" id="1105453at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="600" mass="73607" checksum="F485FC8F16168334" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEL9</accession>
    <name>B7IEL9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydroxydechloroatrazine ethylaminohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_25</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579300.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_25"/>
    <dbReference type="EnsemblBacteria" id="ACJ74533">
        <property type="protein sequence ID" value="ACJ74533"/>
        <property type="gene ID" value="THA_25"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_25"/>
    <dbReference type="eggNOG" id="COG0402">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_2_3_0"/>
    <dbReference type="OMA" id="TWLAHGI"/>
    <dbReference type="OrthoDB" id="1592010at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="1">
        <location>
            <begin position="55"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="452" mass="51283" checksum="B31DF47DD905A6AB" modified="2009-02-10" version="1">MRKLFKNISYIYTFDEKIGDIENGYILVEDNVIKEIGRNWENIEADEVYDLDGYMVIPGFVNTHHHMFQSLTRGLAADKKLFDWLVFHYEIWKFIDEEAIYVSSLIALYEMIKTGVTTTTDHLYLYPYGNNKLFDAEIEAAKLIGVRFHPTRGSMSLSRKNGGLPPDSVVQTDDEILHESVRVIEKYHDPSKYSMLRVALAPCSPFSVTPYLMKETVKISDKYGVLLHTHLAETRDEEEYCLEKFGKRPVDYMEELGWLNNKVWFAHMVWLSKEDMEKLSKNDVGMAHCPSSNMRLGSGIAPVTELKNKIRIGIAVDGSASNDTNNMIMEARNALLLQRAKYGANALTVREVLRMATIGGASVLKMDDYIGKIAPEMAADFIGFKLDKVEFAGGLHNPINSILLCDPKQVDLSVINGEIKILNSEFTSFEISDFLEKHNIISKKLLKKAGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEN6</accession>
    <name>B7IEN6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_42</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MEMO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103367.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_42"/>
    <dbReference type="EnsemblBacteria" id="ACJ74550">
        <property type="protein sequence ID" value="ACJ74550"/>
        <property type="gene ID" value="THA_42"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_42"/>
    <dbReference type="eggNOG" id="COG1355">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038085_2_0_0"/>
    <dbReference type="OMA" id="EQEAQYG"/>
    <dbReference type="OrthoDB" id="1070303at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PTHR11060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="262" mass="30494" checksum="2B4930C84D6221C9" modified="2009-02-10" version="1">MEREMYYADKFYPKNSELLIKFFNQFCKVKEIKNTNSNFGLILPHAGYVYSGKTALYAICKALDFGKPERIIIFGTNHTGIAGNICSLWPRGKWNTPFGYVGIDEELNNYLLKNEIFEENYKAHILEHSIEVLLPILKYYFGSFKFVPIIYNYQSYENTIKIVELLKKIDLKNTLLVASSDFNHYESHEITLRKDEKLIEKILERNIEGIYSLRKSISACGIGPMSVLTMYFDNVKLVYHTTSAEYSKDYTFTVGYASFVLW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEQ6</accession>
    <name>RIMM_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome maturation factor RimM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rimM</name>
        <name type="ordered locus">THA_62</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Binds ribosomal protein S19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The PRC barrel domain binds ribosomal protein S19.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RimM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103395.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IEQ6"/>
    <dbReference type="SMR" id="B7IEQ6"/>
    <dbReference type="STRING" id="484019.THA_62"/>
    <dbReference type="EnsemblBacteria" id="ACJ74570">
        <property type="protein sequence ID" value="ACJ74570"/>
        <property type="gene ID" value="THA_62"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_62"/>
    <dbReference type="eggNOG" id="COG0806">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077636_3_2_0"/>
    <dbReference type="OMA" id="DEFYHAD"/>
    <dbReference type="OrthoDB" id="1497415at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00014">
        <property type="entry name" value="Ribosome_mat_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011961">
        <property type="entry name" value="16S_RimM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002676">
        <property type="entry name" value="RimM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036976">
        <property type="entry name" value="RimM_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01782">
        <property type="entry name" value="RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02273">
        <property type="entry name" value="16S_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_1000196576" description="Ribosome maturation factor RimM">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="PRC barrel" evidence="1">
        <location>
            <begin position="103"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00014"/>
        </source>
    </evidence>
    <sequence length="178" mass="20265" checksum="76DE06B7D6E36C96" modified="2009-02-10" version="1">MINTLRELLSGKVAVAILGKTHGLKGELKLHPFTNFPEIIESLEEIFLYNEKTKQFMVATVENLRLADGYYIIKLNGVENVENARKFVGSKVYINKDELPNLSKDEYYFFEIVGSQVIDESGKVLGVVDEVIQTGSNDVIVVNKNKEDEILIPVIYDYIITLDKENKKIVVKVPEWLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEQ8</accession>
    <name>RS16_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsP</name>
        <name type="ordered locus">THA_64</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103399.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IEQ8"/>
    <dbReference type="SMR" id="B7IEQ8"/>
    <dbReference type="STRING" id="484019.THA_64"/>
    <dbReference type="EnsemblBacteria" id="ACJ74572">
        <property type="protein sequence ID" value="ACJ74572"/>
        <property type="gene ID" value="THA_64"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_64"/>
    <dbReference type="eggNOG" id="COG0228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100590_5_0_0"/>
    <dbReference type="OMA" id="GFYNPIA"/>
    <dbReference type="OrthoDB" id="1937072at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00385">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000307">
        <property type="entry name" value="Ribosomal_S16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020592">
        <property type="entry name" value="Ribosomal_S16_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023803">
        <property type="entry name" value="Ribosomal_S16_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919">
        <property type="entry name" value="PTHR12919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00886">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54565">
        <property type="entry name" value="SSF54565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00002">
        <property type="entry name" value="S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00732">
        <property type="entry name" value="RIBOSOMAL_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000196484" description="30S ribosomal protein S16">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00385"/>
        </source>
    </evidence>
    <sequence length="94" mass="11142" checksum="1E9E2B67A017937F" modified="2009-02-10" version="1">MVRIRLTRMGKKKQPFYRIVVVDQRKRRDGAYIESLGYYDPIKDPYILNVDVDKAVEWIMKGAQPSDTVRNLLRKAGVFKKVDELKRTKKEENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEQ9</accession>
    <name>B7IEQ9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Signal recognition particle protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Fifty-four homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ffh</name>
        <name evidence="10" type="ordered locus">THA_65</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="7">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579325.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_65"/>
    <dbReference type="EnsemblBacteria" id="ACJ74573">
        <property type="protein sequence ID" value="ACJ74573"/>
        <property type="gene ID" value="THA_65"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_65"/>
    <dbReference type="eggNOG" id="COG0541">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_6_0_0"/>
    <dbReference type="OMA" id="DTAGRHK"/>
    <dbReference type="OrthoDB" id="656414at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004780">
        <property type="entry name" value="SRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="PTHR11564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF7">
        <property type="entry name" value="PTHR11564:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="SSF47446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00959">
        <property type="entry name" value="ffh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54" evidence="9">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="105"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="186"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="244"/>
            <end position="247"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="435" mass="48867" checksum="37CE889C1385F14E" modified="2009-02-10" version="1">MFEGLQEKLSKAFKTLSGKGKITEKNIKEAIKMVKLSLLEADVNYKVVKEFIAEVTEKATGEEVLKSLTPDQMFIKILRDELIRVMGEKSQLSLVHSPSYIMMVGLQGSGKTTSAAKLANLLKKKGKKTFLVAADTYRPAAIDQLITLGKKIDVPVFYGDRKDPVKIVREAIKEVKDSGYNVVIFDTAGRLHVDDEMMNELEEIKDILNPDEILMVVDAMAGQDAVNSAKIFNERLDVTGYVVTKMDGDARGGVILSIRYITNKPIKFIGVGEKIEDLEEFHPERIASRILGLGDVLSLIEKAEQELDKDKMQKLGKKMLNAEFTLEDFQEQLKEIKKLGSLSKIVEMLPGSPKVDINQSEKELKVIEAIINSMTPEERRNPKILNASRKKRIAKGSGTSVQEVNKLLKNYEEMKKMMKIFKKGKLPFNLKGFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEX9</accession>
    <name>B7IEX9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alkaline phosphatase synthesis sensor protein PhoR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_135</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579368.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_135"/>
    <dbReference type="EnsemblBacteria" id="ACJ74643">
        <property type="protein sequence ID" value="ACJ74643"/>
        <property type="gene ID" value="THA_135"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_135"/>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_2_0"/>
    <dbReference type="OMA" id="IFRINGR"/>
    <dbReference type="OrthoDB" id="1827824at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="195"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="28"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="424" mass="49126" checksum="4774CB4649CAA5CF" modified="2009-02-10" version="1">MFLEVSTILITIILLIIIAYFRKKLSKMKIYKEALNKLAQLIEEEKDSPPLYIAEKIRKKITKLEEENYDLELSLKNHLTILNNIVDPIIITKKDGTITFANNEARKITRPGIEERKVYEVFEDYYVNQMFDETINNKSLTSGEINIFVNGEKKYYEVKIVPVTLEQNNERFIIVLHDVTQERVLENVRKEFISNVSHELRTPLTSIHGYAEALLEDDLEDKELIRKFLSIIESEAARMTRLINDLLDLEKLESGNTQFVFEKINFTEVLEHVRNIIEPLAKDYQVEVEFVYPDELYLVGDKDRLTQMTLNLVDNAIKYTSLKEKGKKKVTVEAWKENNSIKLVVKDTGVGIPEKAQKKLFERFYRVDKARSRKMGGTGLGLSIVKTIVEKHNGVIEFTSKEGVGTKFVVTLPINREEDNNETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF11</accession>
    <name>B7IF11_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aspartate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="3">1.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-L-aspartate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579386.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_170"/>
    <dbReference type="EnsemblBacteria" id="ACJ74675">
        <property type="protein sequence ID" value="ACJ74675"/>
        <property type="gene ID" value="THA_170"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_170"/>
    <dbReference type="eggNOG" id="COG0136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049966_0_1_0"/>
    <dbReference type="OMA" id="CEEEMKM"/>
    <dbReference type="OrthoDB" id="1799040at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005986">
        <property type="entry name" value="Asp_semialdehyde_DH_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01296">
        <property type="entry name" value="asd_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="4">
        <location>
            <begin position="2"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="322" mass="35963" checksum="C13022D5A5F8E74A" modified="2009-02-10" version="1">MKIGIVGATGEVGRTMIKVLEDFKIPVNELKLFASKRSSGKTLTFNNASIVVEELTEDSMIQKFDFLLFSAGSTVSKIFAPIAAKAGNIVIDNSSAFRMEKEIPLVVPEINGHLLKNYRGIIANPNCSTIQMVLSLYKIHEKFKINEIFVSTYQAVSGAGYKAISELQRQLEGDKSKTVFPKQISHNVIPLIGEIEEDGFSTEEHKMINETRKILDDYSINVYPTTVRVPVFYGHSEAIVVKTKLPFNDISFIENQIKNSKNVILTNEIITPIDVAGSDYVYVSRLRKIDEFTFSFWNVADNIRVGAATNAIRILMLMVGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF20</accession>
    <name>B7IF20_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Apolipoprotein N-acyltransferase</fullName>
            <shortName evidence="8">ALP N-acyltransferase</shortName>
            <ecNumber evidence="8">2.3.1.269</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">lnt</name>
        <name evidence="10" type="ordered locus">THA_179</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the phospholipid dependent N-acylation of the N-terminal cysteine of apolipoprotein, the last step in lipoprotein maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a glycerophospholipid + N-terminal S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein] = a 2-acyl-sn-glycero-3-phospholipid + H(+) + N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48228"/>
            <dbReference type="Rhea" id="RHEA-COMP:14681"/>
            <dbReference type="Rhea" id="RHEA-COMP:14684"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136912"/>
            <dbReference type="ChEBI" id="CHEBI:140656"/>
            <dbReference type="ChEBI" id="CHEBI:140657"/>
            <dbReference type="ChEBI" id="CHEBI:140660"/>
            <dbReference type="EC" id="2.3.1.269"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Protein modification; lipoprotein biosynthesis (N-acyl transfer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CN hydrolase family. Apolipoprotein N-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.269" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579394.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_179"/>
    <dbReference type="EnsemblBacteria" id="ACJ74684">
        <property type="protein sequence ID" value="ACJ74684"/>
        <property type="gene ID" value="THA_179"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_179"/>
    <dbReference type="eggNOG" id="COG0815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019563_1_2_0"/>
    <dbReference type="OMA" id="YVALVPW"/>
    <dbReference type="OrthoDB" id="1291198at2"/>
    <dbReference type="UniPathway" id="UPA00666"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07571">
        <property type="entry name" value="ALP_N-acyl_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01148">
        <property type="entry name" value="Lnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004563">
        <property type="entry name" value="Apolipo_AcylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045378">
        <property type="entry name" value="LNT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20154">
        <property type="entry name" value="LNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00546">
        <property type="entry name" value="lnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="CN hydrolase" evidence="9">
        <location>
            <begin position="215"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="484" mass="55784" checksum="AFED052C71C3C035" modified="2009-02-10" version="1">MIFAIISSILTSLSMPGFLWGGIVWFSLIFLFKSMENRKLSFLYAFLYEYLLIFISMYWVIPILTKNLPEFFGKFSSITGFFVYLLLCLVEAIPFLLFGALYGYFIDRIKGDIPKALFVSSAFTFSEFLRGIGELGFTGIRLSDALYKDIGIIQIASITGTLGIVFLIVFINYILYIKRSKIQVIIITISFIYLANWIVSTNLPLNSANVPIVAVQTNYEQQIKYTSKSEKIMYDIENLIANTPNNYLHILPEAVFATEDIRNSQLEEKLKNISKRKPIILGFPTYDSQPKNSALVYANGNLVGKYDKIKLFPFVEFLPYEKFFKKFEFLKGVAYFTPGDEFKTFKIDGFPEFGIQICFESYFPEISRNLSNNGAKVLFVITNDGWYKHDTALWQHFSKIVFRAIENQRYVVQVSNTGISGVVDNFGRIQKILPLRNQTTGILNIKPKNTKTLYQKFGDWFSILSILLALLIPLFYRDRRKNRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF50</accession>
    <name>B7IF50_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable exodeoxyribonuclease VII small subunit, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579416.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_209"/>
    <dbReference type="EnsemblBacteria" id="ACJ74714">
        <property type="protein sequence ID" value="ACJ74714"/>
        <property type="gene ID" value="THA_209"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_209"/>
    <dbReference type="eggNOG" id="ENOG5033BDN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173972_0_0_0"/>
    <dbReference type="OMA" id="EYFQNER"/>
    <dbReference type="OrthoDB" id="1990165at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009318">
        <property type="term" value="C:exodeoxyribonuclease VII complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008855">
        <property type="term" value="F:exodeoxyribonuclease VII activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037004">
        <property type="entry name" value="Exonuc_VII_ssu_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116842">
        <property type="entry name" value="SSF116842"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="89" mass="10754" checksum="3C254999ABE58C3B" modified="2009-02-10" version="1">MEDILSLEIEDMEKLDFNELVEKIEIVKNYFHKNDVDIEVAIKLYGKAVDLLAVARKKLINFKKEKEEIDKKYMEFLERLEQENEEELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF59</accession>
    <name>B7IF59_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_218</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_218"/>
    <dbReference type="EnsemblBacteria" id="ACJ74723">
        <property type="protein sequence ID" value="ACJ74723"/>
        <property type="gene ID" value="THA_218"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_218"/>
    <dbReference type="eggNOG" id="COG0392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039146_3_0_0"/>
    <dbReference type="OMA" id="RFVEYLI"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="233" mass="26503" checksum="93E8EE6D2C07A469" modified="2009-02-10" version="1">MSIGGQPYQIYHLSKVGVKTEDATNIVISRTLEMSFVVLIIDILSIRPILSAYPKSFGLSLILTGFIVSFSISLLILIGFINKKFLEKILSFFGKFSREKLEREKIIEWIDSLHESIKILWVKNPWILVLDVILYFVTILLYTYIFYIFVDKFSGIEYLYLLGIISLLNSVAYYIPTPGSSGGIEGTYQIVFSQILGPDVSIRIITVYRVVTFYIPLLLGSVFIAKISTNEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF87</accession>
    <name>B7IF87_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_249</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579442.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_249"/>
    <dbReference type="EnsemblBacteria" id="ACJ74751">
        <property type="protein sequence ID" value="ACJ74751"/>
        <property type="gene ID" value="THA_249"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_249"/>
    <dbReference type="eggNOG" id="COG2516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054041_0_0_0"/>
    <dbReference type="OMA" id="NRPFYNE"/>
    <dbReference type="OrthoDB" id="940969at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="18"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="297" mass="34297" checksum="3A44ABBB749DA6E3" modified="2009-02-10" version="1">MVLRASYGTLSLLGLVKNSKIKMDTAYFMLDGRCMFNCAFCAHARDARTENKFLSRIVWKEISVDMIEKLDLKRICLQVVSYKGYKEDLENLLLKLNGKKVSVSVRALSMKEIDRYFELGADAIGLSVDVVNKELFEKIRGGKFENTLELIKNAAKRYPGHITTHVIVGLGETDKELIEFFYMMKELNVLVALFSFTPIKGTAFENLPSPSMERYRKIQLARYLIFEEDVPKDVFVFNKERLEKINYPFKKGIGKAFLTSGCSHCTRPYYNEKPSKEPYNYFEYANNLEEIAKKVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF98</accession>
    <name>B7IF98_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Riboflavin synthase, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ribE</name>
        <name evidence="4" type="ordered locus">THA_261</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579452.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_261"/>
    <dbReference type="EnsemblBacteria" id="ACJ74762">
        <property type="protein sequence ID" value="ACJ74762"/>
        <property type="gene ID" value="THA_261"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_261"/>
    <dbReference type="eggNOG" id="COG0307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034388_2_2_0"/>
    <dbReference type="OMA" id="HFVTGHV"/>
    <dbReference type="OrthoDB" id="1276859at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00402">
        <property type="entry name" value="Riboflavin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001783">
        <property type="entry name" value="Lumazine-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026017">
        <property type="entry name" value="Lumazine-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098">
        <property type="entry name" value="PTHR21098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00677">
        <property type="entry name" value="Lum_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000498">
        <property type="entry name" value="Riboflavin_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00187">
        <property type="entry name" value="ribE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51177">
        <property type="entry name" value="LUMAZINE_BIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Lumazine-binding" evidence="3">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="2">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="2">
        <location>
            <begin position="87"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="3">
        <location>
            <begin position="87"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="179" mass="20214" checksum="DF6A8599F3BE1A97" modified="2009-02-10" version="1">MFTGIIEYVAKDFKIVGNTLEIKLPFSCNVGDSVAVNGICLTVKKVKGDIYYFDLGYETIEKTNIRKAKYLNIERALRAGDRLNGHFVLGHVDGVITFLERETMNSGMLYKFSMPKENYAIKRKGSISLNGISLTIANVSLDTFEVQVIPFTNENTNLKYLKTGDIVNYEIDVFARYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFA9</accession>
    <name>B7IFA9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103856.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_273"/>
    <dbReference type="EnsemblBacteria" id="ACJ74773">
        <property type="protein sequence ID" value="ACJ74773"/>
        <property type="gene ID" value="THA_273"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_273"/>
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    </dbReference>
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    <dbReference type="OMA" id="MDRSIFM"/>
    <dbReference type="OrthoDB" id="145977at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004612">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001272">
        <property type="entry name" value="PEP_carboxykinase_ATP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01293">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="572" mass="65899" checksum="E87D64D4B990109F" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFC0</accession>
    <name>B7IFC0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
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            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579470.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_284"/>
    <dbReference type="EnsemblBacteria" id="ACJ74784">
        <property type="protein sequence ID" value="ACJ74784"/>
        <property type="gene ID" value="THA_284"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_284"/>
    <dbReference type="HOGENOM" id="CLU_959548_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857377" evidence="1">
        <location>
            <begin position="20"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="290" mass="32929" checksum="4C40D4455BB00D4D" modified="2009-02-10" version="1">MRKLLTLMLSFLLFSFFFAEEVIFDGNISVKFSVDSSPKLSTFTTNNFMYVGVKGKSYFIGFLSQNSTYPYMFTIDQAYGIFNFGFANIFLGTKIQNFRVSTLGNDLWLGGNISTEIPFEIGSLYVAIDSNFSDSDSPKVNVLSLNLNLENLIIVNSVFYNNYSNLETGIEIPYSNSKILGYFKYDILNSILKKLTFGFNTKIEKADLTLSFTPDVSNFQSFEIDLSGLYTLNNNYQFGSKIDYNSKSSNINLNFYSNYKYNDILVVPSISWENKNPYAISGALELSFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFE4</accession>
    <name>B7IFE4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, biotin repressor family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_308</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579487.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_308"/>
    <dbReference type="EnsemblBacteria" id="ACJ74808">
        <property type="protein sequence ID" value="ACJ74808"/>
        <property type="gene ID" value="THA_308"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_308"/>
    <dbReference type="eggNOG" id="COG1827">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108798_0_0_0"/>
    <dbReference type="OMA" id="HTPEQTK"/>
    <dbReference type="OrthoDB" id="1652220at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036094">
        <property type="term" value="F:small molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1340.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035922">
        <property type="entry name" value="3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004173">
        <property type="entry name" value="3H_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026043">
        <property type="entry name" value="NadR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40068">
        <property type="entry name" value="PTHR40068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02829">
        <property type="entry name" value="3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037847">
        <property type="entry name" value="NiaR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75500">
        <property type="entry name" value="SSF75500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0533">Nickel</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_11" evidence="3">
        <location>
            <begin position="4"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="3H" evidence="2">
        <location>
            <begin position="69"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR037847-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="168" mass="19001" checksum="D680F5AEFCC39138" modified="2009-02-10" version="1">MKERLKKILSILENSNKPVKGRDLAEVLKVSRQVIVQDISVLKTKGYKIYSTREGYILEKKRDVVRKMIAVKHSEDEIYDELLKIVKAGGKVIDVIVEHPLYGEIKGRLDIETMDDISKFMALMESSNATPLLKLSGGVHIHTIEAPNKKIMNDVLDAISKYLLGVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFH9</accession>
    <name>B7IFH9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_343</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_343"/>
    <dbReference type="EnsemblBacteria" id="ACJ74843">
        <property type="protein sequence ID" value="ACJ74843"/>
        <property type="gene ID" value="THA_343"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_343"/>
    <dbReference type="HOGENOM" id="CLU_3067153_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="53" mass="6508" checksum="941D650E888D3D48" modified="2009-02-10" version="1">MLVFFNKLEKIANFEGKLAYLEFYYFVFLLYTYYRIISIVKKCKILHAKNCKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFI6</accession>
    <name>B7IFI6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Dihydroorotate dehydrogenase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">THA_351</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579517.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_351"/>
    <dbReference type="EnsemblBacteria" id="ACJ74850">
        <property type="protein sequence ID" value="ACJ74850"/>
        <property type="gene ID" value="THA_351"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_351"/>
    <dbReference type="eggNOG" id="COG0167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1145">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042042_4_3_0"/>
    <dbReference type="OMA" id="MVPCVEE"/>
    <dbReference type="OrthoDB" id="1109542at2"/>
    <dbReference type="UniPathway" id="UPA00070"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.26.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023359">
        <property type="entry name" value="Dihydro_DH_chainA_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01037">
        <property type="entry name" value="pyrD_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="304"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="334"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ74850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="360" mass="39585" checksum="00B4BCD45C3E22C2" modified="2009-02-10" version="1">MDLSTNISGIKIDNPVMPASGPLVGDYEKIMFIDSMGVGAIVTKTISTKAAQVPRPCIYGENNFAMNAELWSELPPEKWIDEILPKLKKNLKKPLIVSAGYSKEDMEKLIPQLDPFADAFEISTHYVGKDYNTIAEIVKTIRKNTKKPIFMKISPHIPDPVEFTKVAIGNGANGIVAINSLGPTMKIDIKNRKVLIGNEKGQVWLSGPAIKPLALAIVKMLRDAFPDITIIGVGGIKSADDVIEFLLAGADAVQLLSSALIYGKDIYERIIKDLPRKLEKYGFNSVEEIRKTKLDIPNVKYIANNPIVDHEKCTLCKICEKVCPYFAITIDTKVHVDPNKCFGCGLCESRCPVKAISGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFJ7</accession>
    <name>B7IFJ7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_367</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104061.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_367"/>
    <dbReference type="EnsemblBacteria" id="ACJ74861">
        <property type="protein sequence ID" value="ACJ74861"/>
        <property type="gene ID" value="THA_367"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_367"/>
    <dbReference type="eggNOG" id="COG2608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134973_5_3_0"/>
    <dbReference type="OMA" id="CKMNIER"/>
    <dbReference type="OrthoDB" id="2061355at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="2">
        <location>
            <begin position="3"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="68" mass="7772" checksum="77A0EE9679350FE4" modified="2009-02-10" version="1">MGKFILKVPDMSCKHCVMRITKALEELGEKNFEVKLESKTVEIETENLENVKAKLSEIDYPVESVESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFM2</accession>
    <name>B7IFM2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Pyruvate, phosphate dikinase</fullName>
            <ecNumber evidence="4 7">2.7.9.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ppdK</name>
        <name evidence="14" type="ordered locus">THA_393</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + phosphate + pyruvate = AMP + diphosphate + H(+) + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10756"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 7 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.1" evidence="4 7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579543.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_393"/>
    <dbReference type="EnsemblBacteria" id="ACJ74886">
        <property type="protein sequence ID" value="ACJ74886"/>
        <property type="gene ID" value="THA_393"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_393"/>
    <dbReference type="eggNOG" id="COG0574">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015345_0_2_0"/>
    <dbReference type="OMA" id="RRFVQMY"/>
    <dbReference type="OrthoDB" id="997616at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050242">
        <property type="term" value="F:pyruvate, phosphate dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006090">
        <property type="term" value="P:pyruvate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010121">
        <property type="entry name" value="Pyruvate_phosphate_dikinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931">
        <property type="entry name" value="PTHR22931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000853">
        <property type="entry name" value="PPDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="SSF52009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01828">
        <property type="entry name" value="pyru_phos_dikin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PPDK_N" evidence="12">
        <location>
            <begin position="22"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="PPDK_N" evidence="12">
        <location>
            <begin position="301"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers" evidence="11">
        <location>
            <begin position="427"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers_C" evidence="13">
        <location>
            <begin position="525"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="8">
        <location>
            <position position="460"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="837"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="567"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="623"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="751"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="772"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="773"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="774"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ74886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="881" mass="97972" checksum="2C8BD3B47870C8C8" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFM8</accession>
    <name>B7IFM8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_399</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104133.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_399"/>
    <dbReference type="EnsemblBacteria" id="ACJ74892">
        <property type="protein sequence ID" value="ACJ74892"/>
        <property type="gene ID" value="THA_399"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_399"/>
    <dbReference type="eggNOG" id="ENOG5033GRC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152012_0_0_0"/>
    <dbReference type="OMA" id="YCQKCLM"/>
    <dbReference type="OrthoDB" id="1644436at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="145" mass="16853" checksum="2D6551E1923185CA" modified="2009-02-10" version="1">MSKCERCGKEAELVIRTFVNFVPKSISICKKCLKETLLYDTTNYTKAGIELLAAHVDYIEETQSDSSIYHFKKNNLEIISLMPLAVQSVMFKQDELTKQRITKDLKSRKIYFLKQKLEKAVKNENYELAKHIKEILDSLDNLSEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-22" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFN3</accession>
    <name>XER_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyrosine recombinase THA_404</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">THA_404</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the 'phage' integrase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579552.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFN3"/>
    <dbReference type="SMR" id="B7IFN3"/>
    <dbReference type="STRING" id="484019.THA_404"/>
    <dbReference type="EnsemblBacteria" id="ACJ74897">
        <property type="protein sequence ID" value="ACJ74897"/>
        <property type="gene ID" value="THA_404"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_404"/>
    <dbReference type="eggNOG" id="COG4974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_9_2_0"/>
    <dbReference type="OMA" id="HRFLYAE"/>
    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0159">Chromosome partition</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0229">DNA integration</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000215961" description="Tyrosine recombinase THA_404">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Core-binding (CB)" evidence="3">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="107"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="2">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P0A8P8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="283" mass="32995" checksum="BC46A689C8A585CA" modified="2009-02-10" version="1">MDKVIEMFSDYLAHVRRHSENTLKAYKKDVRKFLTYVGKQINNIERKDVEEFLKALSKGDITGESPRETTISRYISSLNSFFNYLELSGMIYENPMERIKHPRIRRKIPDFLTEDEVSSLIGAFNEENELRQKTAISLLYYAGLRISELCNLRTTDISFIPPFLRVEMGKGRKDRLVPLPDKVIPILKKYIDLENPKIFVFENGKKHVHPSTVFRWLKEGVKRANIKKDVHPHTLRHSYATHLIRKGVNIKVVQELLGHTNLSTTSIYLHVADQEKFDAVKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFP1</accession>
    <name>B7IFP1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF190 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_412</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0166 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579560.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_412"/>
    <dbReference type="EnsemblBacteria" id="ACJ74905">
        <property type="protein sequence ID" value="ACJ74905"/>
        <property type="gene ID" value="THA_412"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_412"/>
    <dbReference type="eggNOG" id="COG1993">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146749_0_1_0"/>
    <dbReference type="OMA" id="EHWNTLR"/>
    <dbReference type="OrthoDB" id="1984529at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003793">
        <property type="entry name" value="DUF190"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02641">
        <property type="entry name" value="DUF190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF190" evidence="2">
        <location>
            <begin position="2"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="105" mass="12138" checksum="EA94B1BDC92968E6" modified="2009-02-10" version="1">MKLLRVYLGEKDFEKGIPTAEYIMKLAYKEGMKGVTILKGIMGFGKKRHIHRSDFFSISEDLPVVIDIVDEAEKINMFVEKLKELNFDCLIVEIPVVAHYMESKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-22" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFP7</accession>
    <name>PRMA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal protein L11 methyltransferase</fullName>
            <shortName evidence="1">L11 Mtase</shortName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">prmA</name>
        <name type="ordered locus">THA_418</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Methylates ribosomal protein L11.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-lysyl-[protein] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:54192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13826"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. PrmA family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579565.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFP7"/>
    <dbReference type="SMR" id="B7IFP7"/>
    <dbReference type="STRING" id="484019.THA_418"/>
    <dbReference type="EnsemblBacteria" id="ACJ74911">
        <property type="protein sequence ID" value="ACJ74911"/>
        <property type="gene ID" value="THA_418"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_418"/>
    <dbReference type="eggNOG" id="COG2264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049382_0_2_0"/>
    <dbReference type="OMA" id="WNALVIK"/>
    <dbReference type="OrthoDB" id="1712450at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00735">
        <property type="entry name" value="Methyltr_PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004498">
        <property type="entry name" value="Ribosomal_PrmA_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000401">
        <property type="entry name" value="RPL11_MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000212760" description="Ribosomal protein L11 methyltransferase">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00735"/>
        </source>
    </evidence>
    <sequence length="260" mass="29820" checksum="484307CFB78D7C44" modified="2009-02-10" version="1">MNKVFNEFVYKISQDQIEKIEEYFFENNIKNYYFYETKEGTFLVLVFEEKQEKIFLPFNLEFVENRTTTSEDWVKNLITKPFEFIEGVYVDPDHNNVDGEIVIRITPGLAFGTGLHDTTKLSAKFLKKYLRPGMDVLDLGCGSAILSILAKKLGADRVLGVDNDPLAVEAAKENVERNNVDVEIRQSDLFSNVDGKFDLIVSNIIAEILIEALKDLPKFLKKDGVVILSGIIDSKLPLFKNYNIVEHWRSNEWNALVIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFS2</accession>
    <name>B7IFS2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Imidazolonepropionase</fullName>
            <ecNumber evidence="2">3.5.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hutI</name>
        <name evidence="6" type="ordered locus">THA_445</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.7" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579585.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_445"/>
    <dbReference type="EnsemblBacteria" id="ACJ74936">
        <property type="protein sequence ID" value="ACJ74936"/>
        <property type="gene ID" value="THA_445"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_445"/>
    <dbReference type="eggNOG" id="COG1228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041647_0_1_0"/>
    <dbReference type="OMA" id="CAPHARW"/>
    <dbReference type="OrthoDB" id="785930at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050480">
        <property type="term" value="F:imidazolonepropionase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005920">
        <property type="entry name" value="HutI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42752">
        <property type="entry name" value="PTHR42752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01224">
        <property type="entry name" value="hutI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0369">Histidine metabolism</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="5">
        <location>
            <begin position="243"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="398" mass="44590" checksum="4F0F1988CA47296B" modified="2009-02-10" version="1">MLRIHAEHLISPSGQAPKKGNEMKKIFEAFDVDIILNKGKIVDIKKHKTTDDFTIKAKLVTPAFVDAHTHIPFFGSRAKEFYLRARGKSYSEIFANGGGIHSSVRMLRNATIDEIVKQNLKYLSLFKRHGIAAVEGKSGYGLEKFSELKQLKAMKILNEIQDVKVIPTFLGLHAIPIDANKKDYIDEVKKWLDDIKEFTDTIDVFCDKGVFLPQDIEEFFEFAKNKGFKIRFHADEIENVGAAKLAIKLGAISADHLLKINDDDISQIANSNTVATLMPGTSFYLGEDFAPARKLIDNGAAVSIGSDFNPGSCPIFNPAFIFHLALRFLKMEPEEILTAYTLNSSYVLGIENGKIEPGYKCDIAIWNTNELLDIPYMFDQNFLSAIIINGKVTIYESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFS9</accession>
    <name>B7IFS9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hyaluronan synthase related, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_452</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579591.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_452"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ74943">
        <property type="protein sequence ID" value="ACJ74943"/>
        <property type="gene ID" value="THA_452"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_452"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_733143_0_0_0"/>
    <dbReference type="OMA" id="KRWMRGA"/>
    <dbReference type="OrthoDB" id="724641at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="46"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="379" mass="44585" checksum="CD10952646D00E85" modified="2009-02-10" version="1">MLFTVSFYILNILISLIIVLFGKYKNEKRTNYLHENRDPEYNPLVTVIIPTYNEEDVIENTIKTVEKSSYKNIEIIVVDDNSKDNTFKIAKSLEKIYPNLKVIQKKGKKGKPQSINEAMEFAKGEIILLIDADARIPENYISSHIFCFSNDKVNMIFTNFEPYNFKFKPVYIIQELYFNFVKAIFYSNIFVKMIFMGNGVFFRRTLLEKILPIDPDTLVDDFSMATKLSKMKVKEYYSTYPFIKIQYATNFKDLWNQHKRWYVGGFREMFKRLKEGNIAFLFYYLLVGLIVFLPIISIVLDILFKIGMLKITAGFIIAVYILMWVSSLDSRQFGIFSFPYSFLFVPLLIVFEYLVLLLSYIIGPFSKAVEWYKVKRVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFT0</accession>
    <name>B7IFT0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_453</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579592.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_453"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ74944">
        <property type="protein sequence ID" value="ACJ74944"/>
        <property type="gene ID" value="THA_453"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_453"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025996_17_4_0"/>
    <dbReference type="OMA" id="CFLHADT"/>
    <dbReference type="OrthoDB" id="1445278at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="248" mass="28493" checksum="C1AB9ABCD56F47A3" modified="2009-02-10" version="1">MKISIIVPVLNEEKILENTLKSIINQSYKNFELIVVDNGSTDRSVEIASKYTDNILFESKKGSIHAMSKGFKNATGDILVTCDADSIYPENYLEKIVKSFMRDSKVCAVYGPFSLIEENKFKNFFTILTYILLDFLSRLFTKTYIVGAANFAIKKEAYEKVGGYDTKSNLASQDFRLAKKLSKVGKVKFVPSLIVKTSNRRLKEEGFFKSMKKAFKFWADVAFNLNKIKYDNYYSKEYYKKKGVKNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFW0</accession>
    <name>B7IFW0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Positive regulator of sigma(E), RseC/MucC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579610.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_483"/>
    <dbReference type="EnsemblBacteria" id="ACJ74974">
        <property type="protein sequence ID" value="ACJ74974"/>
        <property type="gene ID" value="THA_483"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_483"/>
    <dbReference type="eggNOG" id="COG3086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124911_1_0_0"/>
    <dbReference type="OMA" id="ECESCAM"/>
    <dbReference type="OrthoDB" id="1998173at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="135" mass="15278" checksum="B5834B9C45E7E51F" modified="2009-02-10" version="1">MKELFTIAKVDEKYIYLERDTSGCSSCAISGSCNVKSVEQLKVEKDNKFEYFPGDFVILDLKYRPALLAFLLYGLPVILLVLGVSLGTYLKLSDYFSFLIGLLFMSGAFAIFRIWDKRYKPKIVDVRHVNKGGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFZ2</accession>
    <name>B7IFZ2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Periplasmic serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_517"/>
    <dbReference type="EnsemblBacteria" id="ACJ75006">
        <property type="protein sequence ID" value="ACJ75006"/>
        <property type="gene ID" value="THA_517"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_517"/>
    <dbReference type="eggNOG" id="COG0616">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067083_0_0_0"/>
    <dbReference type="OMA" id="DKIIMDP"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002825">
        <property type="entry name" value="Pept_S49_ser-pept_pro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01972">
        <property type="entry name" value="SDH_sah"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="291" mass="32867" checksum="ABC9185B5CDA39E2" modified="2009-02-10" version="1">MSRAIIERGGKMGDLFTIIFQIFWMVLIISSFAPLFKSLSLHSSRDGLIRAIEKKRGSRVITLIHREESLNFFGLPIRRYIDIEDSEELIRAIKMTPNDMPIDLIIHTPGGLVLAAEQIANALKKHKGKVTVFVPHYAMSGGTLIALAADEIVMDENAVLGPIDPQLGQYPAASILSVVKKKDINEIDDQTLILADVAEKAMKQMKEYATNLLKEKYEENAEELADKLVSGYWTHDYPITVEKARELGLKVSTDMPNEIYLLMSLYKQSGKRRPSVNYVPVDYRVFKDNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG26</accession>
    <name>B7IG26_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SHOCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_552</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100591.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_552"/>
    <dbReference type="EnsemblBacteria" id="ACJ75040">
        <property type="protein sequence ID" value="ACJ75040"/>
        <property type="gene ID" value="THA_552"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_552"/>
    <dbReference type="eggNOG" id="COG3462">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159099_2_0_0"/>
    <dbReference type="OMA" id="VIHYSSK"/>
    <dbReference type="OrthoDB" id="2010019at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018649">
        <property type="entry name" value="SHOCT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09851">
        <property type="entry name" value="SHOCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="SHOCT" evidence="2">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="86" mass="10428" checksum="5CF0F17BC4220031" modified="2009-02-10" version="1">MHWFYPVWYHGFYGWIIPVIGLVLFILVVFLFYRILKNLIKDFNEKTSSSYNFSNDESLKILNERFARGEISEDEYKRIKSLILKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG50</accession>
    <name>PAND_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate 1-decarboxylase</fullName>
            <ecNumber evidence="1">4.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartate alpha-decarboxylase</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName evidence="1">Aspartate 1-decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">Aspartate 1-decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">panD</name>
        <name type="ordered locus">THA_577</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the pyruvoyl-dependent decarboxylation of aspartate to produce beta-alanine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + L-aspartate = beta-alanine + CO2</text>
            <dbReference type="Rhea" id="RHEA:19497"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="EC" id="4.1.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="1">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; (R)-pantothenate biosynthesis; beta-alanine from L-aspartate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterooctamer of four alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Is synthesized initially as an inactive proenzyme, which is activated by self-cleavage at a specific serine bond to produce a beta-subunit with a hydroxyl group at its C-terminus and an alpha-subunit with a pyruvoyl group at its N-terminus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PanD family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100547.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IG50"/>
    <dbReference type="SMR" id="B7IG50"/>
    <dbReference type="STRING" id="484019.THA_577"/>
    <dbReference type="EnsemblBacteria" id="ACJ75064">
        <property type="protein sequence ID" value="ACJ75064"/>
        <property type="gene ID" value="THA_577"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_577"/>
    <dbReference type="eggNOG" id="COG0853">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115305_2_1_0"/>
    <dbReference type="OMA" id="MLYSKIH"/>
    <dbReference type="OrthoDB" id="1751990at2"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00002"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004068">
        <property type="term" value="F:aspartate 1-decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006523">
        <property type="term" value="P:alanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06919">
        <property type="entry name" value="Asp_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00446">
        <property type="entry name" value="PanD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003190">
        <property type="entry name" value="Asp_decarbox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21012">
        <property type="entry name" value="PTHR21012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02261">
        <property type="entry name" value="Asp_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006246">
        <property type="entry name" value="Asp_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00223">
        <property type="entry name" value="panD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_1000192053" description="Aspartate 1-decarboxylase beta chain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_1000192054" description="Aspartate 1-decarboxylase alpha chain" evidence="1">
        <location>
            <begin position="25"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="1">
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="73"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser)" evidence="1">
        <location>
            <position position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00446"/>
        </source>
    </evidence>
    <sequence length="120" mass="13451" checksum="6D2ED58E8B95D5FA" modified="2009-02-10" version="1" precursor="true">MQEIMLKSKIHMAKVTDKSINYMGSIGIDTELLEKAGMKPYELVLVADVNNGQRFVTYIIPEEKGSKKIVINGAAARLVEQGDRVIIMAFGMYDSNEYKGPKVIIMNEKNEIVEIKQEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG71</accession>
    <name>B7IG71_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_598</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_598"/>
    <dbReference type="EnsemblBacteria" id="ACJ75085">
        <property type="protein sequence ID" value="ACJ75085"/>
        <property type="gene ID" value="THA_598"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_598"/>
    <dbReference type="HOGENOM" id="CLU_3318377_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="39" mass="4685" checksum="FB58AB44B90D994C" modified="2009-02-10" version="1">MLKLYNFSDKSLSKNNFTTDKYSQGILSIDTYRMYTKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGC9</accession>
    <name>B7IGC9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thermonuclease, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579712.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_666"/>
    <dbReference type="EnsemblBacteria" id="ACJ75143">
        <property type="protein sequence ID" value="ACJ75143"/>
        <property type="gene ID" value="THA_666"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_666"/>
    <dbReference type="eggNOG" id="COG1525">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082132_0_0_0"/>
    <dbReference type="OMA" id="PNNDDRE"/>
    <dbReference type="OrthoDB" id="1719840at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="SSF50199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TNase-like" evidence="1">
        <location>
            <begin position="38"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="295" mass="34479" checksum="D123D2772ABF6B62" modified="2009-02-10" version="1">MDLKKLLPILLFSVLITLFSCIDIDAIDFLSPIKDDSGFVKLDVDHIVDGDTFYGYVNGEYKKVRIVGIDTPETHAGSKPIGEYGEDAKRFLENFVSKYEIYLKIVGNDEYRRTLAYVFGKNSENGYIFYEESVLMEGYARPLIYLNNDDRELTPNNDDRELTPNNDDRELTPRIVQAYYYAFENRKGIFSKWNSAKVLSSKAQITSLLEGKIVFLEGTVKDIRKNYHRDGGYTYYIDCDWFEISIRDGEYEYFFKDYNLYSIKGKKVRFYGELWFDDNKPEILLRSPNEIVVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGE9</accession>
    <name>B7IGE9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Clostripain-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_691</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579731.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_691"/>
    <dbReference type="EnsemblBacteria" id="ACJ75163">
        <property type="protein sequence ID" value="ACJ75163"/>
        <property type="gene ID" value="THA_691"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_691"/>
    <dbReference type="eggNOG" id="COG1716">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034651_0_0_0"/>
    <dbReference type="OMA" id="WDHGSAW"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005077">
        <property type="entry name" value="Peptidase_C11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37835">
        <property type="entry name" value="PTHR37835"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03415">
        <property type="entry name" value="Peptidase_C11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="507" mass="58747" checksum="38D31457049788CB" modified="2009-02-10" version="1">MKKKVLSIITILMFLFLFLGCTNIFNESAYKILADNEEHTTGRISYIDVKVVDKNNNPVSGVRVRFLKKTSVDASEEELYDVISGQYEVTTNEEGVAKIALLMEKSYDSLDIHAYVVDDPLSEVNFKVTYKEPKWFFIVWMSADNDLEYYGLGDLNEMKNNNENVSVIVFFDGKDEKDKILFLDENGNLYQSLWQGREEINSGDPNELFEALIYSSKFESKRRSLIIWNHGNAWVSDSQSYVSKGISYDETSGEDIAVVELKEVLEKYNGKDYPKIDLLGMDACLMGSFEVLYDLKDLTDYVVASSFPEPGEGWNYQFFGEIAQDDEAQEVGKKIIDTFKEYYSKTDQKNNELSLALYDMSKLSDGSNYISLLGERLSNIMDDSVRNSIKSFYNSITQYYRDNKLGNLLVDLNEFIDFIKNNINDPNVKFLSEEIQNSLKSFILYWYSEWHSEENQKLIDYPVSIFIPENLETLEYYAEDYNTLEFTLENKWDEFLESFLESGDILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGG0</accession>
    <name>B7IGG0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycerol-3-phosphate acyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Acyl-PO4 G3P acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Acyl-phosphate--glycerol-3-phosphate acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">G3P acyltransferase</fullName>
            <shortName evidence="10">GPAT</shortName>
            <ecNumber evidence="10">2.3.1.275</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Lysophosphatidic acid synthase</fullName>
            <shortName evidence="10">LPA synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">plsY</name>
        <name evidence="11" type="ordered locus">THA_703</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl phosphate + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:34075"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="2.3.1.275"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Probably interacts with PlsX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the PlsY family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.275" evidence="10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104590.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_703"/>
    <dbReference type="EnsemblBacteria" id="ACJ75174">
        <property type="protein sequence ID" value="ACJ75174"/>
        <property type="gene ID" value="THA_703"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_703"/>
    <dbReference type="eggNOG" id="COG0344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081254_6_1_0"/>
    <dbReference type="OMA" id="TFGVWSA"/>
    <dbReference type="OrthoDB" id="1691856at2"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043772">
        <property type="term" value="F:acyl-phosphate glycerol-3-phosphate acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01043">
        <property type="entry name" value="PlsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003811">
        <property type="entry name" value="G3P_acylTferase_PlsY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30309">
        <property type="entry name" value="PTHR30309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02660">
        <property type="entry name" value="G3P_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01207">
        <property type="entry name" value="G3P_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="165"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="196" mass="22786" checksum="96395D985536E1A0" modified="2009-02-10" version="1">MFEFFIVILQFLSGSMMYSYLLGKICGVDIRVLGDGNPGVSNLWKMKGWKFGLIGLFLDFSKGFFPVILFKDHLNYWVYIIALSFSVLGHAFSPFLNFKGGKAITTSFGVWTAATIWKAPIIMGSVLVILKTIFKNKKSSDFDFLIFLFGFLSLIPYIMYEKSLYLYYLYFFNFFVVILKHRKEIKNIWRDLLGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGG7</accession>
    <name>B7IGG7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Diguanylate cyclase (Ggdef) domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579745.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_710"/>
    <dbReference type="EnsemblBacteria" id="ACJ75181">
        <property type="protein sequence ID" value="ACJ75181"/>
        <property type="gene ID" value="THA_710"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_710"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_552917_0_0_0"/>
    <dbReference type="OMA" id="EKQGEDQ"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="353"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="474" mass="56481" checksum="7B73FD2A9A70B0FB" modified="2009-02-10" version="1">MLIFLFILTFVLISLLFYIVRIIKLTKLIYNTARKDSNVIIWKNSRILYCDKPLMELLKKHGIDVNDFRNFSFEKINFYINKYPFLKEFFEKFRVNFEEKLFEYETTIFLSNEFYKIKFRREKNKTTVYSVLLITNVILNIKEYTDELISSIYKIPEEFFNLTTSDTSTYELVDYFFNFLSSQNIIDSLVVGIRKIDGSINIIYGKIGDKKFKNFTIKDKTLISYISDTGEKLYVNNSEELKLPEEYKLMKVINKPYSIYGIPLRIGENVFGAVLFEKEGNNKFCLKDFHLFEILAFLISISIKLKNEYQILYKSSKENFKKSIIDPLTKAYNRNYLSIVLYKNFEISKEKSKYKIVIFLDLDNFKSLNDKYGHVYGDKVLKNFVKTAKMVLRKDDIIIRYGGDEFLILLYDLNIENAESVIQRIRKMILNSEFKVDFSYGLMYFDHEKSLEENIKIVDKKMYEMKSKKKKLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGH6</accession>
    <name>B7IGH6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA-binding protein HU 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_722</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104645.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_722"/>
    <dbReference type="EnsemblBacteria" id="ACJ75190">
        <property type="protein sequence ID" value="ACJ75190"/>
        <property type="gene ID" value="THA_722"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_722"/>
    <dbReference type="eggNOG" id="COG0776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_3_1_0"/>
    <dbReference type="OMA" id="IYVRGFG"/>
    <dbReference type="OrthoDB" id="1847472at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020816">
        <property type="entry name" value="Histone-like_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00045">
        <property type="entry name" value="HISTONE_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="92" mass="10404" checksum="FB179BBD95238F52" modified="2009-02-10" version="1">MSKKELVNMIAEKIPELKKKDIKAVVDAVFESISDALAKGEKVQLIGFGTFEVRKAEERTGVNPRTREKIKIPARKVPKFKPGKELKEKVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGK7</accession>
    <name>B7IGK7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579780.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_755"/>
    <dbReference type="EnsemblBacteria" id="ACJ75221">
        <property type="protein sequence ID" value="ACJ75221"/>
        <property type="gene ID" value="THA_755"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_755"/>
    <dbReference type="eggNOG" id="COG3877">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132137_0_0_0"/>
    <dbReference type="OMA" id="YPTAKAR"/>
    <dbReference type="OrthoDB" id="1943427at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018658">
        <property type="entry name" value="DUF2089"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09862">
        <property type="entry name" value="DUF2089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="124" mass="14061" checksum="E5ED732FFF50C0CC" modified="2009-02-10" version="1">MLPKCPVCGKPMKVTQLKCVHDNVTVSGMFTVSPLAFLEEEDMNFIILFLRSRGNLKEMERVTGIGYFTLRGRLEKLLDKMGLKPIGDSEVDDQDDVFEKLKKKLISVEDALNIIKRKGGEKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGP4</accession>
    <name>B7IGP4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Uridylate kinase</fullName>
            <shortName evidence="6">UK</shortName>
            <ecNumber evidence="6">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Uridine monophosphate kinase</fullName>
            <shortName evidence="6">UMP kinase</shortName>
            <shortName evidence="6">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">pyrH</name>
        <name evidence="8" type="ordered locus">THA_796</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + UMP = ADP + UDP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="6">Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the UMP kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100371.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_796"/>
    <dbReference type="EnsemblBacteria" id="ACJ75258">
        <property type="protein sequence ID" value="ACJ75258"/>
        <property type="gene ID" value="THA_796"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_796"/>
    <dbReference type="eggNOG" id="COG0528">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033861_0_0_0"/>
    <dbReference type="OMA" id="PIIVFDM"/>
    <dbReference type="OrthoDB" id="1043239at2"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04254">
        <property type="entry name" value="AAK_UMPK-PyrH-Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_B">
        <property type="entry name" value="PyrH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015963">
        <property type="entry name" value="Uridylate_kinase_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02075">
        <property type="entry name" value="pyrH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="7">
        <location>
            <begin position="3"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="8"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="129"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="233" mass="26133" checksum="7EAB788AAE900C01" modified="2009-02-10" version="1">MYKRVLLKLSGEVLSGEGEKGFNIDKIIYLIDELKSVLEYGTNIGIVIGAGNLFRGREMKELSPTIADQIGMLGTVINALYLKDLFEKHGLRTVVVSQVTSLPSIRPIHYDDINLYFDAGYLVIFAGGTSNPFFTTDTAAALRAVEMKADILIKGTKVSGIFDKDPKKYEDAIKFDKLTFDEAIEKELKIMDTEAFSICRRYNMKILVMDFFKKGNLLRAIREENVGTLVVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGP5</accession>
    <name>B7IGP5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA_15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_797</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579802.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_797"/>
    <dbReference type="EnsemblBacteria" id="ACJ75259">
        <property type="protein sequence ID" value="ACJ75259"/>
        <property type="gene ID" value="THA_797"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_797"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4717">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_367577_0_0_0"/>
    <dbReference type="OMA" id="ANFIRYC"/>
    <dbReference type="OrthoDB" id="370464at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041685">
        <property type="entry name" value="AAA_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13175">
        <property type="entry name" value="AAA_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_15" evidence="3">
        <location>
            <begin position="1"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="158"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="486"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="818" mass="96713" checksum="7632172E006446F1" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGQ2</accession>
    <name>B7IGQ2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_804</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100385.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_804"/>
    <dbReference type="EnsemblBacteria" id="ACJ75266">
        <property type="protein sequence ID" value="ACJ75266"/>
        <property type="gene ID" value="THA_804"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_804"/>
    <dbReference type="eggNOG" id="COG1399">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100236_1_1_0"/>
    <dbReference type="OMA" id="RLDHRNP"/>
    <dbReference type="OrthoDB" id="1665714at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003772">
        <property type="entry name" value="YceD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02620">
        <property type="entry name" value="YceD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="183" mass="21328" checksum="A0533EFD14A0A3DF" modified="2009-02-10" version="1">MKWKIKIKDIIAKKNVKIEDYYESEYIELHTGTYKVIDKRFKVKISIVFTDDTIVVGGYVSGKVERPCDRCLEMTVMPLEGTIEAVYDLKNEPNFKNSEIENLKNVIYYKGEEIDLEERILEALVVSAPDVFVCKEDCKGLCPFCGENLNENPDHVCKEMEVFNIDPRFEKLLKLKEEMQNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGQ9</accession>
    <name>B7IGQ9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flagellar basal body protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_811</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579813.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_811"/>
    <dbReference type="EnsemblBacteria" id="ACJ75273">
        <property type="protein sequence ID" value="ACJ75273"/>
        <property type="gene ID" value="THA_811"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_811"/>
    <dbReference type="eggNOG" id="COG4786">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013687_0_0_0"/>
    <dbReference type="OMA" id="NLYFAIQ"/>
    <dbReference type="OrthoDB" id="947161at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019776">
        <property type="entry name" value="Flagellar_basal_body_rod_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020013">
        <property type="entry name" value="Flagellar_FlgE/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037925">
        <property type="entry name" value="FlgE/F/G-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117143">
        <property type="entry name" value="SSF117143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03506">
        <property type="entry name" value="FlgEFG_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00588">
        <property type="entry name" value="FLAGELLA_BB_ROD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="3">
        <location>
            <begin position="11"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="4">
        <location>
            <begin position="173"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="235" mass="26807" checksum="F23E7D3FB8931682" modified="2009-02-10" version="1">MYKGVYLASMGMLADITKLDTLSNNISNAETIGYKADNLAFKTYLEKRLHSFKPMPQEKKVEIKDIGKFEQAVILDEVKTDFSQGNIEHTENMLDFAIDGSGFFVVEKNGEKLYTRAGNFKISSDGFLVTSEGYYVLDKNDQRIKVFDKSDLINNIKVVDLDNPRKIGYTFYKGQEIEKENFRILQGYVEKSNVDIVKEMVKMIEANRHYEVLSKAVTVHDELLNKSINSAGNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGR5</accession>
    <name>B7IGR5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_817</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100416.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_817"/>
    <dbReference type="EnsemblBacteria" id="ACJ75279">
        <property type="protein sequence ID" value="ACJ75279"/>
        <property type="gene ID" value="THA_817"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_817"/>
    <dbReference type="eggNOG" id="COG4198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031277_0_0_0"/>
    <dbReference type="OMA" id="IYIADGH"/>
    <dbReference type="OrthoDB" id="591335at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008323">
        <property type="entry name" value="UCP033563"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36454">
        <property type="entry name" value="PTHR36454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06245">
        <property type="entry name" value="DUF1015"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033563">
        <property type="entry name" value="UCP033563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="412" mass="47764" checksum="D65D65FDD792C002" modified="2009-02-10" version="1">MIVRPFRGLRPKKNMIEKVAAKPYDVVTTEEARKEAERNPYTFYKVTRPEINFDREVDTHSDEVILKGKEVLEEFQKNGIMILEDKPAIYVYKEEWKGLSQIGIYATFSTKEYRDGKIKKHELTRKDKEDERARHVKLLKAHTGPVFLMYRSKPELDTLIIKHTSKIPEYDYVDETGIHHTLWVVKDENEINEIVEAFKSVDAFYIADGHHRAAAAARAAEEFAKENPNHTGNEEYNFFLAVLFPHNQLNILDYNRVVKDLNGLSEEEFLNKVSEKFDVERVEKSPYKPTKKHEFGMYLNKKWYKLTPKKGTFDENDVVSSLDVSILQENLLNPILGIENPRTDKRIDFVGGIKGIEELERLVDSGEFKVAFALYPTSIDDLLKVADEGKIMPPKSTWFEPKLKSGIIVHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGS6</accession>
    <name>B7IGS6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_828</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100438.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_828"/>
    <dbReference type="EnsemblBacteria" id="ACJ75290">
        <property type="protein sequence ID" value="ACJ75290"/>
        <property type="gene ID" value="THA_828"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_828"/>
    <dbReference type="HOGENOM" id="CLU_1609984_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="165" mass="18977" checksum="218D5850FB2525C9" modified="2009-02-10" version="1">MILAFLIIFTVVSFSIPVFIGIEFSNIPSPAFSTDMVLGLNLKVKFDYLYFLAPLIQIDSEGKFVQIRIDENYFTQNLLFGFEFNHNIYKDSVNFMKTSIGGDFPFLNLISEKRVQPLNFRFGLGIGYYSYEINFGKVVRFEFFNANSKNLIYGGIYYLAPGLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGV3</accession>
    <name>B7IGV3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Crispr-associated protein, Csx11 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_857</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579837.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_857"/>
    <dbReference type="EnsemblBacteria" id="ACJ75317">
        <property type="protein sequence ID" value="ACJ75317"/>
        <property type="gene ID" value="THA_857"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_857"/>
    <dbReference type="eggNOG" id="COG1353">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007515_0_0_0"/>
    <dbReference type="OMA" id="ILGIQYD"/>
    <dbReference type="OrthoDB" id="140901at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014055">
        <property type="entry name" value="CRISPR-assoc_prot_Csx11"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02682">
        <property type="entry name" value="cas_csx11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="1060" mass="127005" checksum="AAC681B70E818189" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGW5</accession>
    <name>B7IGW5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sugar transaminase, involved in dTDP-4-amino-4,6-dideoxyglucose biosynthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_869</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104366.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_869"/>
    <dbReference type="EnsemblBacteria" id="ACJ75329">
        <property type="protein sequence ID" value="ACJ75329"/>
        <property type="gene ID" value="THA_869"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_869"/>
    <dbReference type="eggNOG" id="COG0399">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033332_1_0_0"/>
    <dbReference type="OMA" id="INPRRYF"/>
    <dbReference type="OrthoDB" id="1722208at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="PTHR30244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="359" mass="41934" checksum="31612B3B6E5BD975" modified="2009-02-10" version="1">MINVTRSIMPDFEKYTQMIEKLWETRWLTNNGEYLVKLEKALEERFNTKCAVVSNGTLALLIAIELFDFPKDSEIITTPFTFVATSSSIIWQKYKPVFVDINPETFNIDPNEIEKKITENTRAILAVHVFGNPCDIEKIEEIAKKHRLKIIYDAAHCFDVYYKGESIYKFGDVSIASFHATKVFHTIEGGAIFSDNDELIRKAKRLRNFGFNEEIEIEDKGINAKMNEFQAAMGLLNLEIVGKEIKKRKKIYESYKEKLKGLVKFQKLSSNITKYNYIYMPVLFENQEQREKAYKKLKEEGYNTRKYFFPSLDTVFNQENQCKTSQEISKRILHLPLYGDLEEKHVEKICTTIEKVVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGX0</accession>
    <name>B7IGX0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sigma-70 region 2 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579849.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_874"/>
    <dbReference type="EnsemblBacteria" id="ACJ75334">
        <property type="protein sequence ID" value="ACJ75334"/>
        <property type="gene ID" value="THA_874"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_874"/>
    <dbReference type="eggNOG" id="ENOG5033KBK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2157182_0_0_0"/>
    <dbReference type="OMA" id="YQTICYL"/>
    <dbReference type="OrthoDB" id="1767318at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="1">
        <location>
            <begin position="4"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="109" mass="13079" checksum="04193F31931D2E63" modified="2009-02-10" version="1">MDIYKTYKDEVKNITGRYFKKYGYKISSYEDLEQTVWYILLSGVKKFDGRGDLKKFLLSFIKHKLISILKYNRKPPYCQDSPFTPLTSEYISIDDSKSKYFREIHGEYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGX2</accession>
    <name>B7IGX2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_876</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579851.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_876"/>
    <dbReference type="EnsemblBacteria" id="ACJ75336">
        <property type="protein sequence ID" value="ACJ75336"/>
        <property type="gene ID" value="THA_876"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_876"/>
    <dbReference type="eggNOG" id="ENOG502ZVFE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181401_5_0_0"/>
    <dbReference type="OMA" id="LMFRKSE"/>
    <dbReference type="OrthoDB" id="2034992at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021321">
        <property type="entry name" value="DUF2922"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11148">
        <property type="entry name" value="DUF2922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="70" mass="7768" checksum="5687D7573BBF2EB9" modified="2009-02-10" version="1">MKRLSLIYRNTTDGSAYRVNLSDPVDPIDTATLEQDAQVLIDNGLIPAGYTFDEARVLESNTNVLIDLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGX5</accession>
    <name>B7IGX5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Succinoglycan biosynthesis protein ExoA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_879</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579854.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_879"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75339">
        <property type="protein sequence ID" value="ACJ75339"/>
        <property type="gene ID" value="THA_879"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_879"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025996_19_0_0"/>
    <dbReference type="OMA" id="IMHAEGE"/>
    <dbReference type="OrthoDB" id="1445278at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="7"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="323" mass="36952" checksum="279ABF2D0FB1220D" modified="2009-02-10" version="1">MQYPKVSIIIPARNEEKHIEKCLDSIIENDYPNKEIIVVDGKSEDKTREIIEKFENVKIVDNEDKITPVALNLGIQEATGKYVMIAGAHTTYSKNYISSCVKRLEEDKCDVAGGYVVTLPGSNTLTSKAISKVLSHPFGVGGAKYRTKNVKEEYVDTVAYGIYKKEAFEKVGLFAPELKRNQDIEMNLRLKRAGMRILLVPEAKAYYFARSTYKGLFKNNFLNGLWVVLSTHYSRRGFSIRHVVPLLFVLFLIFGSIFSMFSTKIKFTFFLILSLYLILSIFFSLKIALKEKNIKLFFPTLISFWILHISYGLGSFYGLFYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGY0</accession>
    <name>B7IGY0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase, group 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579859.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_884"/>
    <dbReference type="EnsemblBacteria" id="ACJ75344">
        <property type="protein sequence ID" value="ACJ75344"/>
        <property type="gene ID" value="THA_884"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_884"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063441_0_0_0"/>
    <dbReference type="OMA" id="IPTYPYD"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="177"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="364" mass="42882" checksum="C7FCF424C2F7E977" modified="2009-02-10" version="1">MKKKGLVFIRTDLKKHIGVYHKVLEISKTLSLKGNVILVYNFGGKIIFSEYCENKLVYKRKINNVNIIKVIEDLLKKNKFEFAYIRFSLGDPISLIIQKILNKNKVKIFLEIPVFPFDKEIISLKNKIRWYLNSLFFKSEIFLSDYIVSTSYNVDNIFGKPIIRIFNGIHPEKFRLKKKQKLERDFYLIGVSSLTFWQGYDRVIKGIYDFKKKNETYNVKFFIIGEGHERKKLEKMVKKLGLIDNVEFLGVLKGEKLDAFFDMAHIAVGALGRHRTNSNNISTLKASEYCARGIPFFTSVNEIAFSEDFKFMKRIESNDENIDIFEIIDFYKYCENFDYPLEMRQFAMKELSWQKGLKNVLDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH07</accession>
    <name>B7IH07_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_912</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100788.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_912"/>
    <dbReference type="EnsemblBacteria" id="ACJ75371">
        <property type="protein sequence ID" value="ACJ75371"/>
        <property type="gene ID" value="THA_912"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_912"/>
    <dbReference type="eggNOG" id="COG5512">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160523_3_1_0"/>
    <dbReference type="OMA" id="VECESIY"/>
    <dbReference type="OrthoDB" id="2036888at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007922">
        <property type="entry name" value="DUF721/UPF0232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05258">
        <property type="entry name" value="DciA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="101" mass="12016" checksum="A1461BE3E78B36F5" modified="2009-02-10" version="1">MQRLGDVLRELAKKNLFIKNLYVSTLSREHFEDVIGKPFNEHCKVVMFKDGVIYIECDDQLFVTELNFFREKIREKLNDRLGIYAIKKVVIRKKRRAINGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH75</accession>
    <name>B7IH75_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_980</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0718 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100954.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_980"/>
    <dbReference type="EnsemblBacteria" id="ACJ75439">
        <property type="protein sequence ID" value="ACJ75439"/>
        <property type="gene ID" value="THA_980"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_980"/>
    <dbReference type="eggNOG" id="COG0701">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058185_0_0_0"/>
    <dbReference type="OMA" id="MGKDAWW"/>
    <dbReference type="OrthoDB" id="1559099at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005524">
        <property type="entry name" value="DUF318"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03773">
        <property type="entry name" value="ArsP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="362" mass="39562" checksum="A0F13E3233B865E7" modified="2009-02-10" version="1">MKEFVIILTIFLIFYFVPFNASIVQDSILNGFQMLQEYAREHVLLCLVPAFFIAGTISVMLKKDAVLKLLGPTAKRIISYPIAAVSGGILAVCSCTILPLFGGIYKKGAGIGPATTFLFAGPAINIAAIFLTARVLGWDLGLARLIATITAAILIGLIMELLFQEKGEGGFITTEEKSENGLKVLVFFLLQLAFLIIGGLKINPAVKYTTMFVISIIVFLMAVFSFKRDTTKSWLEETWDFAKKILPYLFIGVFLAGIITKLLPQEVVTKILGSNGILSNLFASVIGMFMYFATLTEVPIVQALRELGMAKGPTLALLMAGNSLSLPSMIVITRLFGKKKAFTYFTLVVIFSTIFGLIYGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH81</accession>
    <name>B7IH81_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_986</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579915.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_986"/>
    <dbReference type="EnsemblBacteria" id="ACJ75445">
        <property type="protein sequence ID" value="ACJ75445"/>
        <property type="gene ID" value="THA_986"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_986"/>
    <dbReference type="eggNOG" id="ENOG502ZY0E">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2541468_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="83" mass="9599" checksum="ACEAE691993FEB0A" modified="2009-02-10" version="1">MDDKYISGYFAYGVLYNGEYYPEFSFQTPFSVNKKDYYLGIQLKMSAESDVMFYLSKIDINNPFTIIITNNSGSFFGSFPISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH82</accession>
    <name>B7IH82_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved protein, with A weak D-galactarate dehydratase/altronate hydrolase domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579916.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_987"/>
    <dbReference type="EnsemblBacteria" id="ACJ75446">
        <property type="protein sequence ID" value="ACJ75446"/>
        <property type="gene ID" value="THA_987"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_987"/>
    <dbReference type="eggNOG" id="COG1672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021114_0_1_0"/>
    <dbReference type="OMA" id="IILLDEY"/>
    <dbReference type="OrthoDB" id="191489at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018631">
        <property type="entry name" value="AAA-ATPase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012547">
        <property type="entry name" value="PDDEXK_9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09820">
        <property type="entry name" value="AAA-ATPase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08011">
        <property type="entry name" value="PDDEXK_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA-ATPase_like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="511" mass="61014" checksum="4253A5F9B4E886B9" modified="2009-02-10" version="1">MKKLPIGIQDYKEIIEGNYIYVDKTKYIFDLIDNGKYYFLSRPRRFGKSLTISTLYYIFKGEKELFKDTYIYDKLNFKEYPIIRLNILLAATDNEERFKKSLTNLIKQEGQRNNVEILEEDYKFAFDELIIKLSKKGKVVILVDEYEKPILDNINNKEKAEKYREILRDFYVTIKSRDEYIKFVFITGITKFTKTGVFSALNNLNDISLNRKYSQMLGYTQEELEYYFNEHVEETAKEMGISKEELLKEIKTYYNGFSFDGERFVYNPFSVLRFFDERKFQNYWFESGSPSFIYEYVKGRKIEYEDLVKYTVDSLDFTTREIEDANANIFFAQAGYLTFKGIKKYGITEKYILDYPNLEVKNSFSKLILEANYSLNEETYEKIYEIYELVEENNIEGLIEEIKRIISAIPYNLHQNRESYYHSLIYTILASAGLNVTAEELTNLGRIDLVLEHNDKIYLFEIKLDKSAKEAIEQIKEKKYYEKYKNYEVYIIGVNINSEKRNIDEYIIEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH93</accession>
    <name>B7IH93_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_998</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100995.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_998"/>
    <dbReference type="EnsemblBacteria" id="ACJ75457">
        <property type="protein sequence ID" value="ACJ75457"/>
        <property type="gene ID" value="THA_998"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_998"/>
    <dbReference type="eggNOG" id="COG0210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004585_5_10_0"/>
    <dbReference type="OMA" id="YRHFVVD"/>
    <dbReference type="OrthoDB" id="137860at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="7">
        <location>
            <begin position="6"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="8">
        <location>
            <begin position="288"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="632" mass="72385" checksum="916B2913E069EA2A" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHB6</accession>
    <name>B7IHB6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR-associated endoribonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1022</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1022"/>
    <dbReference type="EnsemblBacteria" id="ACJ75480">
        <property type="protein sequence ID" value="ACJ75480"/>
        <property type="gene ID" value="THA_1022"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1022"/>
    <dbReference type="eggNOG" id="COG1583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089858_2_0_0"/>
    <dbReference type="OMA" id="PVGFNKY"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045747">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36984">
        <property type="entry name" value="PTHR36984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01881">
        <property type="entry name" value="Cas_Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005054">
        <property type="entry name" value="PF1131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005054-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="240" mass="27942" checksum="88BAFB2920F6CAE6" modified="2009-02-10" version="1">MEFVLDVDIILPVGFNKYIQALIYNLFSTKLRKFVHEEGFRGKKKFSLFCFFSILERGKFFKNMKIFNFGKKISFFISSPVNMLMKDLVTNLFKGDEFILGENRIYLSSAMAVIKNVSGDSIKVKALTPIEVHRTYKGSGKNKTRYFTPFESEFVELINLNIKNKWEAFYKEKLDKDIEIVPLFDDERYKSVVYYGFGEKRFVIEGWKGSYLLKGDNEILSFVYDTGLGSKNSQGFGFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHK3</accession>
    <name>B7IHK3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD hydrolase, IIB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580007.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1114"/>
    <dbReference type="EnsemblBacteria" id="ACJ75567">
        <property type="protein sequence ID" value="ACJ75567"/>
        <property type="gene ID" value="THA_1114"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1114"/>
    <dbReference type="eggNOG" id="COG0561">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044146_0_1_0"/>
    <dbReference type="OMA" id="APHNNEE"/>
    <dbReference type="OrthoDB" id="1374424at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="273" mass="31167" checksum="060CA4710C40321C" modified="2009-02-10" version="1">MIKLIITDLDGTLLDDQKNIPPQNAFALRKAMEKGIHVSIATGRNYYSAKRYIDELGLDVPVILQNGAFIYMPLENKVLYESLLESAIAKDLINYARKFELDYILYSYFLDEIDMYMDKEHNGGFVTYIKQNSWRIRYVDDVLRYITREKVAQVVLIGDESKIWYINDIIKQKYPGKYSVVKSTVVDGWSFIEFFGPGSSKEEAVAFLMNYFNVKSEETMFLGDGYNDLGAMKLVGFPVAVDNACAEVKETSRFVTLSNNEGGVAFAIEKFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHL8</accession>
    <name>B7IHL8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580019.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1129"/>
    <dbReference type="EnsemblBacteria" id="ACJ75582">
        <property type="protein sequence ID" value="ACJ75582"/>
        <property type="gene ID" value="THA_1129"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1129"/>
    <dbReference type="eggNOG" id="COG0558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1255446_0_0_0"/>
    <dbReference type="OMA" id="TWMDYEM"/>
    <dbReference type="OrthoDB" id="2122339at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="220" mass="25432" checksum="12CC482C0C881800" modified="2009-02-10" version="1">MRYREIMKYFDTPAAPICYVFVDRVASLFVWLNVNFLKIHPNYISILSGIFTVFSVVNFSKGLFIKGALFYFFAFLFDAIDGPSARALNKTSMLGVILEVWTDSLRLVGSTAGISWYLYKSTLDERWLLILFIWLVSFSAGLWLYPKAKESFKDFKKEKKVEGYYISPVPTWMDYEMMAFFFLPLFGQVKLGMYILVIGYFISSLGMSGYLLFLGGKKKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHS2</accession>
    <name>TRUA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA pseudouridine synthase A</fullName>
            <ecNumber evidence="1">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truA</name>
        <name type="ordered locus">THA_1186</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580066.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHS2"/>
    <dbReference type="SMR" id="B7IHS2"/>
    <dbReference type="STRING" id="484019.THA_1186"/>
    <dbReference type="EnsemblBacteria" id="ACJ75636">
        <property type="protein sequence ID" value="ACJ75636"/>
        <property type="gene ID" value="THA_1186"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1186"/>
    <dbReference type="eggNOG" id="COG0101">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014673_0_1_0"/>
    <dbReference type="OMA" id="FLYGMVR"/>
    <dbReference type="OrthoDB" id="1075262at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02570">
        <property type="entry name" value="PseudoU_synth_EcTruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PTHR11142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000194577" description="tRNA pseudouridine synthase A">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00171"/>
        </source>
    </evidence>
    <sequence length="244" mass="28228" checksum="1973B3A833051B3F" modified="2009-02-10" version="1">MKRFAIEFAYDGTKFFGYQGQPNVRTVQGDLEDALERIFKERIYTQAAGRTDAGVHANGQVAAFNCPIERLTETDIKNALNANLPDDIYVKKAWVVDKSFNPRFAATKRIYHYFISTNEKDVFMRNYVWNFRYDLDIEAMRKAASFLEGEHDFSSFKKGKDEKNPVRTIYRIRILTLKKGLILIRVEGRSFLRSMVRNIVGSLVRVGLGQWKPEKILEVLEKRSRQEAAGTAPPHGLYLYKVLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHV5</accession>
    <name>RS17_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsQ</name>
        <name type="ordered locus">THA_1224</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101457.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHV5"/>
    <dbReference type="SMR" id="B7IHV5"/>
    <dbReference type="STRING" id="484019.THA_1224"/>
    <dbReference type="EnsemblBacteria" id="ACJ75669">
        <property type="protein sequence ID" value="ACJ75669"/>
        <property type="gene ID" value="THA_1224"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1224"/>
    <dbReference type="eggNOG" id="COG0186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073626_1_2_0"/>
    <dbReference type="OMA" id="HPMYGKF"/>
    <dbReference type="OrthoDB" id="2035880at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_B">
        <property type="entry name" value="Ribosomal_S17_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019984">
        <property type="entry name" value="Ribosomal_S17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_S17/S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_S17_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="PTHR10744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03635">
        <property type="entry name" value="uS17_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000143314" description="30S ribosomal protein S17">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <sequence length="99" mass="11402" checksum="D5F50CC6788797A9" modified="2009-02-10" version="1">MPKKKLIGEVVSDKMDKTVVVAVSTLVKHPRVGKYIKRTKKYYAHDENNECRDGDIVEIIESRPLSKLKRWRVLRIVERSVFADETLDEELEGGSSDDN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHV9</accession>
    <name>RS14Z_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S14 type Z</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsZ</name>
        <name evidence="1" type="synonym">rpsN</name>
        <name type="ordered locus">THA_1228</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S3 and S10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101463.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHV9"/>
    <dbReference type="SMR" id="B7IHV9"/>
    <dbReference type="STRING" id="484019.THA_1228"/>
    <dbReference type="EnsemblBacteria" id="ACJ75673">
        <property type="protein sequence ID" value="ACJ75673"/>
        <property type="gene ID" value="THA_1228"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1228"/>
    <dbReference type="eggNOG" id="COG0199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139869_3_0_0"/>
    <dbReference type="OMA" id="RAYTRCN"/>
    <dbReference type="OrthoDB" id="2032609at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01364_B">
        <property type="entry name" value="Ribosomal_S14_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_S14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_S14/S29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_S14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023053">
        <property type="entry name" value="Ribosomal_S14_Z"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836">
        <property type="entry name" value="PTHR19836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000143922" description="30S ribosomal protein S14 type Z">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01364"/>
        </source>
    </evidence>
    <sequence length="61" mass="7292" checksum="D8AAAE5CD7FDD261" modified="2009-02-10" version="1">MARKGLVERWKKPKKFKTREYTRCKICGRAHSVYREFGICRVCFRKMANEGKLPGVRKATW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHX2</accession>
    <name>RS4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsD</name>
        <name type="ordered locus">THA_1241</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101494.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHX2"/>
    <dbReference type="SMR" id="B7IHX2"/>
    <dbReference type="STRING" id="484019.THA_1241"/>
    <dbReference type="EnsemblBacteria" id="ACJ75686">
        <property type="protein sequence ID" value="ACJ75686"/>
        <property type="gene ID" value="THA_1241"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1241"/>
    <dbReference type="eggNOG" id="COG0522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092403_0_2_0"/>
    <dbReference type="OMA" id="NVVFRMG"/>
    <dbReference type="OrthoDB" id="1211060at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_B">
        <property type="entry name" value="Ribosomal_S4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_S4/S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_S4/S9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005709">
        <property type="entry name" value="Ribosomal_S4_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="PTHR11831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF4">
        <property type="entry name" value="PTHR11831:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01017">
        <property type="entry name" value="rpsD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000140805" description="30S ribosomal protein S4">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="98"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <sequence length="209" mass="24502" checksum="614F6BA44CF5CCC0" modified="2009-02-10" version="1">MARYTGPQCKLCRREGMKLYLKGERCFTDKCAFDRRPFAPGDHGRDKKKLTQYGIQLRAKQTMKRIYGVLETQFRRYYETASRKSGDTRENLVVQVERRLDNVVYRLGFAVNRTTARQLVNHGHVLVNGRKVTIPSYQVRPGDVIEIREKSRDILPVKNAIELNKDKNTMPWLTVDYENYKGTYERNPKLEEVIDLPVDVQAIIELYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICL9</accession>
    <name>B7ICL9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1301</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580132.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1301"/>
    <dbReference type="EnsemblBacteria" id="ACJ75746">
        <property type="protein sequence ID" value="ACJ75746"/>
        <property type="gene ID" value="THA_1301"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1301"/>
    <dbReference type="eggNOG" id="ENOG5033C9E">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200617_0_0_0"/>
    <dbReference type="OMA" id="ERAMLMR"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035205">
        <property type="entry name" value="DUF5320"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17253">
        <property type="entry name" value="DUF5320"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="74" mass="8332" checksum="FD5F5F9703E617EB" modified="2009-02-10" version="1">MPFYGGGYGGGYGRGFGIGYGRGFGRGFGYGAGYGYYGPSIEEERAMLMRYLEEELRFVEERLRELDNYQGGDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICN4</accession>
    <name>RL21_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplU</name>
        <name type="ordered locus">THA_1316</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds to 23S rRNA in the presence of protein L20.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L20.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580143.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ICN4"/>
    <dbReference type="SMR" id="B7ICN4"/>
    <dbReference type="STRING" id="484019.THA_1316"/>
    <dbReference type="EnsemblBacteria" id="ACJ75761">
        <property type="protein sequence ID" value="ACJ75761"/>
        <property type="gene ID" value="THA_1316"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1316"/>
    <dbReference type="eggNOG" id="COG0261">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061463_3_2_0"/>
    <dbReference type="OMA" id="HRQEITR"/>
    <dbReference type="OrthoDB" id="1949059at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01363">
        <property type="entry name" value="Ribosomal_L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036164">
        <property type="entry name" value="L21-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028909">
        <property type="entry name" value="L21p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001787">
        <property type="entry name" value="Ribosomal_L21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018258">
        <property type="entry name" value="Ribosomal_L21_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349">
        <property type="entry name" value="PTHR21349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00829">
        <property type="entry name" value="Ribosomal_L21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141091">
        <property type="entry name" value="SSF141091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00061">
        <property type="entry name" value="L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01169">
        <property type="entry name" value="RIBOSOMAL_L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000143861" description="50S ribosomal protein L21">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01363"/>
        </source>
    </evidence>
    <sequence length="106" mass="12200" checksum="8F60B745C94F7E9C" modified="2009-02-10" version="1">MYAIVESGGKQFRVEPGKVFFTERVVGKGEGEEVKLDKVLMVKTDDGKVLVGKPYLENVEIKGTIVEHGRARKVIVGKFRPRKNYRRIKGHRQWYTAIKVEEIQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICT7</accession>
    <name>B7ICT7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Type IV pilin-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1369</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580185.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1369"/>
    <dbReference type="EnsemblBacteria" id="ACJ75814">
        <property type="protein sequence ID" value="ACJ75814"/>
        <property type="gene ID" value="THA_1369"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1369"/>
    <dbReference type="eggNOG" id="COG2165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149241_0_0_0"/>
    <dbReference type="OMA" id="YRIRYIN"/>
    <dbReference type="OrthoDB" id="1960029at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045584">
        <property type="entry name" value="Pilin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54523">
        <property type="entry name" value="SSF54523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00409">
        <property type="entry name" value="PROKAR_NTER_METHYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="138" mass="15300" checksum="9C01B52AAE3B2041" modified="2009-02-10" version="1">MKKGFTLIELLIVLAVISALLSVATPVALRSVAKAKATQVAMNLRNVNSALEQALLLYPDEFNPTALSGKNILFELYNKGFINVDLSTKGYQVVYDDKDKKYLIKYTNNDIDPILVKSAYPAVKQNDDGELYVEVSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICV5</accession>
    <name>B7ICV5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nudix hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580197.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1388"/>
    <dbReference type="EnsemblBacteria" id="ACJ75832">
        <property type="protein sequence ID" value="ACJ75832"/>
        <property type="gene ID" value="THA_1388"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1388"/>
    <dbReference type="eggNOG" id="COG0494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062658_5_1_0"/>
    <dbReference type="OMA" id="DYQVHPG"/>
    <dbReference type="OrthoDB" id="1831818at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="36"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="180" mass="20545" checksum="E0198AE2C84DC944" modified="2009-02-10" version="1">MEKKISSKEIFKGLLLHVKKDEVMLENGRKSFREYVLHPGAVAVVPILDDNKVVLVKQFRYPIGKQLLEIPAGKFDFKDEDPLECAKRELKEETGFDAKDYLYLGYIHTTPGFSNEVIHLYLARNLESGQSNPDEDEIIDVEIEDFNTVLQKCINGEITDAKTIAGIFKAYFMLRSENNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICV7</accession>
    <name>B7ICV7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flagellar secretion chaperone FliS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fliS</name>
        <name evidence="7" type="ordered locus">THA_1390</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FliS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580199.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1390"/>
    <dbReference type="EnsemblBacteria" id="ACJ75834">
        <property type="protein sequence ID" value="ACJ75834"/>
        <property type="gene ID" value="THA_1390"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1390"/>
    <dbReference type="eggNOG" id="COG1516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080373_4_2_0"/>
    <dbReference type="OMA" id="EFRDTWK"/>
    <dbReference type="OrthoDB" id="1730695at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16098">
        <property type="entry name" value="FliS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003713">
        <property type="entry name" value="FliS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036584">
        <property type="entry name" value="FliS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34773">
        <property type="entry name" value="PTHR34773"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02561">
        <property type="entry name" value="FliS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039090">
        <property type="entry name" value="Flis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101116">
        <property type="entry name" value="SSF101116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00208">
        <property type="entry name" value="fliS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR039090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="136" mass="15728" checksum="B59806667FA9FFE3" modified="2009-02-10" version="1">MDYTEQMVKTASPAKLIEMLYQRAVELLDMAERDVKNKEYVKANEEIQKCQDIITELNLSLDLEKGGEIAKNLRALYTYMFKTLVEANTKKDVEKIREVRGYLSELLETWREAMKNVGNTASKVPDPNRPRLNVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICW5</accession>
    <name>B7ICW5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101786.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1398"/>
    <dbReference type="REBASE" id="19390">
        <property type="entry name" value="M.TafTCDamP"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75842">
        <property type="protein sequence ID" value="ACJ75842"/>
        <property type="gene ID" value="THA_1398"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1398"/>
    <dbReference type="eggNOG" id="COG0742">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075826_0_2_0"/>
    <dbReference type="OMA" id="FNWLMPY"/>
    <dbReference type="OrthoDB" id="1827334at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031167">
        <property type="term" value="P:rRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004398">
        <property type="entry name" value="RNA_MeTrfase_RsmD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004553">
        <property type="entry name" value="CHP00095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00095">
        <property type="entry name" value="TIGR00095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="179" mass="20059" checksum="A8EBEBDC94D21568" modified="2009-02-10" version="1">MLYIESGELKGRKIELVPNANTRYTPGKVRLALVSMLDLKGKKVLELCAGSGVVGFEFLSNGASNVFFVDVSIRSIKTIEKNAKNLNVKDNIKVIKKDARIFLKSAKEKFDIVFMDPPFNLGIVNELLNFVGNVLKEDSILIVEHSKKEKLTPPDYLEVLSSKKYGDVLIDLFKLKNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID10</accession>
    <name>B7ID10_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutamate synthase, small subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gltD</name>
        <name evidence="3" type="ordered locus">THA_1444</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580226.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1444"/>
    <dbReference type="EnsemblBacteria" id="ACJ75887">
        <property type="protein sequence ID" value="ACJ75887"/>
        <property type="gene ID" value="THA_1444"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1444"/>
    <dbReference type="eggNOG" id="COG0543">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_1_0_0"/>
    <dbReference type="OMA" id="AGQFIIL"/>
    <dbReference type="OrthoDB" id="1109542at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="278" mass="31535" checksum="8F1A73CD6556F251" modified="2009-02-10" version="1">MKNKILEKRKLAYGVHEFWIENPTVSKNAKPGQFVIFRLYENGERIPLTISAINKDSFRVIVKAIGKSTYELCRLNKGDFILDVVGPLGMPSEVKYYGNVLVVGGGVGIAAIAPIVKELLKEKNNVDVILGGRNKEYIILEDEFKNANNIYIVTDDGSLGEKAYPHQFMEKLMQEKKYDIIWAIGPSKMMKACSEVAKKHDIKIWVSLNSIMVDGTGMCGGCRVRIGNELKYTCVDGPEFDGRLVDWESFEKRLNQYREEEKIALSRYLQEVGEPTWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID23</accession>
    <name>B7ID23_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580236.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1457"/>
    <dbReference type="EnsemblBacteria" id="ACJ75900">
        <property type="protein sequence ID" value="ACJ75900"/>
        <property type="gene ID" value="THA_1457"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1457"/>
    <dbReference type="eggNOG" id="COG1426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1539299_0_0_0"/>
    <dbReference type="OMA" id="MDGWKEI"/>
    <dbReference type="OrthoDB" id="2066702at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="174" mass="20189" checksum="2846C141EC20C849" modified="2009-02-10" version="1">MDGWKEISEILKKEVISSNLSLLEFSKRVGIDINTFRKYYEGNFSGEPSIVEEHLRKIKEVFNLKEDLIMLYELGSSKRIKDSKISKSNLYIFLIFTVFLFIGSVILFLNVYETPLVRLNSIGDEVKVNGKVVKTFFMDEGEYIVEGPSLLKKINKETKKVVMEKYKVVVGWEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID45</accession>
    <name>B7ID45_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-N-acetylhexosaminidase</fullName>
            <ecNumber evidence="3">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1479</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580253.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1479"/>
    <dbReference type="CAZy" id="GH20">
        <property type="family name" value="Glycoside Hydrolase Family 20"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75922">
        <property type="protein sequence ID" value="ACJ75922"/>
        <property type="gene ID" value="THA_1479"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1479"/>
    <dbReference type="eggNOG" id="COG3525">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020160_1_0_0"/>
    <dbReference type="OMA" id="DKYCKER"/>
    <dbReference type="OrthoDB" id="727559at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102148">
        <property type="term" value="F:N-acetyl-beta-D-galactosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015882">
        <property type="entry name" value="HEX_bac_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600">
        <property type="entry name" value="PTHR22600"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02838">
        <property type="entry name" value="Glyco_hydro_20b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="SSF55545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_20b" evidence="7">
        <location>
            <begin position="3"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_20" evidence="6">
        <location>
            <begin position="128"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="496"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="599" mass="69424" checksum="C777B3A3CCE16C0A" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID70</accession>
    <name>B7ID70_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small GTP-binding protein domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1506</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102027.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1506"/>
    <dbReference type="EnsemblBacteria" id="ACJ75947">
        <property type="protein sequence ID" value="ACJ75947"/>
        <property type="gene ID" value="THA_1506"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1506"/>
    <dbReference type="eggNOG" id="COG0486">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042017_0_0_0"/>
    <dbReference type="OMA" id="PQDIQAP"/>
    <dbReference type="OrthoDB" id="1203651at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023873">
        <property type="entry name" value="FeFe-hyd_GTPase_HydF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041606">
        <property type="entry name" value="HydF_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040644">
        <property type="entry name" value="HydF_tetramer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18128">
        <property type="entry name" value="HydF_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18133">
        <property type="entry name" value="HydF_tetramer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03918">
        <property type="entry name" value="GTP_HydF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="11"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="HydF_dimer" evidence="2">
        <location>
            <begin position="174"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="HydF_tetramer" evidence="3">
        <location>
            <begin position="275"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="399" mass="44545" checksum="2E7D6118E8540CE9" modified="2009-02-10" version="1">MIASGGFRKYIAITGRRNVGKSSFMNALIGQDVSIVSNVAGTTTDPVFKSMELSPIGPVTLIDTPGLDDIGELGQKRIEKAKKTLYRADCGILIVDSQPKEYEHQIVEIFKQLEIPYIIVINKIDTINSDEVEETYKKFNVPIVKVSALKKIGFENIGNVIDSVIPKDDEIPYLSDLIDGGDLVVLVVPIDLGAPKGRLIMPQVHAIREGLDKEALVLVVKERELRYALENIGMKPKIVVTDSQSVMKVVSDVPEEIKLTTFSILESRYRGDLNYFVESVKHIENLKDGDKVIIMEGCTHRPLTEDIGRVKIPRWLTNHTGAALEFKVWAGVDMPELNEIEDAKLVIHCGGCVMNRNSMMRRVRMFKRLGIPMTNYGVAISYLHGVLERALSPFKDVEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-01" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID82</accession>
    <name>Y1518_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleotide-binding protein THA_1518</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">THA_1518</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Displays ATPase and GTPase activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RapZ-like family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ACJ75959" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75959.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102048.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ID82"/>
    <dbReference type="SMR" id="B7ID82"/>
    <dbReference type="STRING" id="484019.THA_1518"/>
    <dbReference type="EnsemblBacteria" id="ACJ75959">
        <property type="protein sequence ID" value="ACJ75959"/>
        <property type="gene ID" value="THA_1518"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1518"/>
    <dbReference type="eggNOG" id="COG1660">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059558_0_0_0"/>
    <dbReference type="OrthoDB" id="1603998at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00636">
        <property type="entry name" value="RapZ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005337">
        <property type="entry name" value="RapZ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30448">
        <property type="entry name" value="PTHR30448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03668">
        <property type="entry name" value="ATP_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005052">
        <property type="entry name" value="P-loopkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000383300" description="Nucleotide-binding protein THA_1518">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="57"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="279" mass="32209" checksum="4735B6991146DB5A" modified="2009-09-01" version="2">MKNLIILTGLSGAGKSTALGLLEDMGFYCIDNLPINLVETILPVISMNVDNIAFVLDARSENFERIDEILNNLKKKYASNLKVIFLTAKDAIIINRFAHTRRNHPLLKRVNSLEKAIQLEREILTKALEFSDIVIDTSNLNPHQLREKLVEILGSVKKKFLVRILSFGFKYGLPLDVDFIFDVRFFPNPFYIKDLREKSGRDEKVKEFLYNTQGVKEYIEMIKNVVDFALKRYENEGRMELSIGIGCTGGQHRSVFFAEELASIYRQRYEVLLEHRDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDF0</accession>
    <name>B7IDF0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HTH domain superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102336.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1589"/>
    <dbReference type="EnsemblBacteria" id="ACJ76027">
        <property type="protein sequence ID" value="ACJ76027"/>
        <property type="gene ID" value="THA_1589"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1589"/>
    <dbReference type="eggNOG" id="COG2345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_203781_1_0_0"/>
    <dbReference type="OMA" id="KRCYYAP"/>
    <dbReference type="OrthoDB" id="2053299at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_11" evidence="1">
        <location>
            <begin position="3"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="59" mass="6606" checksum="2667699ACB3215B0" modified="2009-02-10" version="1">MEKEKVLEVLKKEGKPMKAGEIAEKLGVDSKVVSKLISELKKEGKIESPKRCYYAPKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDG2</accession>
    <name>B7IDG2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TRK system potassium uptake protein TrkH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1601</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580289.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1601"/>
    <dbReference type="EnsemblBacteria" id="ACJ76039">
        <property type="protein sequence ID" value="ACJ76039"/>
        <property type="gene ID" value="THA_1601"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1601"/>
    <dbReference type="eggNOG" id="COG0168">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030708_3_0_0"/>
    <dbReference type="OMA" id="GSMNHFS"/>
    <dbReference type="OrthoDB" id="756220at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="404"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="458"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="493" mass="54782" checksum="F503810798224FD7" modified="2009-02-10" version="1">MPRKKEQYKLIIHNIGLVLMYYTIIILFPLVFNTFYPNELKNSYAFLLSAFLSFFIGYILFKATKDVSSTTVTVREGAVIVFFTWLLVVLIGSIPFILVENLTFSQAVFESTSGFTTTGLTMFTDVSKVSKLILVWRSIMQFIGGAGFALIMMGSVIGPKGFGLYHAEGRVDNIAPNIKRSARIITLIYITYAFLGTVLLDIVGMPLFDAFNHSLTALATGGFSVKNASIGDYNNLRVEIVTIILMFLGGTGFGVHYTLWRGNFKAFLKNGEPWLMLTTIFTFSLIMAFNGIGKVFSTFSQGFRESLFQTTSALTGTGFSTVDLTNPLWINFGLGMFILTSLMMAGGGMDSTAGGLKQYRIWVTIKVLLHSVKEFLLPSKSVTKISAWKGQNKNFIKNEDIKEIFLVFSMYFFTFFVGSLILSSYGYNLTQSMFEFASAMNGVGLSSGITSPNMPLGAMWTLTIAMFTGRLEFLVVIYAVAKMINDLYESIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDG8</accession>
    <name>B7IDG8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="8">1.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_1607</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580293.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1607"/>
    <dbReference type="EnsemblBacteria" id="ACJ76045">
        <property type="protein sequence ID" value="ACJ76045"/>
        <property type="gene ID" value="THA_1607"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1607"/>
    <dbReference type="eggNOG" id="COG0057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030140_0_2_0"/>
    <dbReference type="OMA" id="NCVAPMA"/>
    <dbReference type="OrthoDB" id="944149at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43148">
        <property type="entry name" value="PTHR43148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gp_dh_N" evidence="9">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="4">
        <location>
            <begin position="149"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="4">
        <location>
            <begin position="208"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="10"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="6">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ76045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="332" mass="36088" checksum="EFB3ADBDED66F4F3" modified="2009-02-10" version="1">MKIAINGFGRIGRLVLRELIRRNSDIEVVAINDLTDAATLAHLFKYDSVHKVLPNEVKATENSIIIDGKEIKIFAERDPENLPWKDLGVDVVVESTGVFRNREGAEKHLKAGAKKVVITAPAKGEDITIVFGCNEEQLSSEHNIISCASCTTNSIASIAKIINDEFKIVTGHLITVHSYTNDQRILDLPHKDLRRARAAAVNIIPTTTGAAKAVAVVVPELKGKLDGMAIRVPTPDGSLTGLSVVVEKATSAEEVNEVIKKATEGKLKGIIGYNTEPIVSSDIVGTTFAGIFDATLTKVMNGTLVNVFSWYDNEYGYTNRVVDTLELVEKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDH6</accession>
    <name>B7IDH6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cell division protein FtsA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ftsA</name>
        <name evidence="7" type="ordered locus">THA_1615</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Cell division protein that is involved in the assembly of the Z ring. May serve as a membrane anchor for the Z ring.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with FtsZ.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FtsA/MreB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580298.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1615"/>
    <dbReference type="EnsemblBacteria" id="ACJ76053">
        <property type="protein sequence ID" value="ACJ76053"/>
        <property type="gene ID" value="THA_1615"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1615"/>
    <dbReference type="eggNOG" id="COG0849">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037850_4_1_0"/>
    <dbReference type="OMA" id="YEMFYNF"/>
    <dbReference type="OrthoDB" id="1044637at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020823">
        <property type="entry name" value="Cell_div_FtsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003494">
        <property type="entry name" value="SHS2_FtsA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02491">
        <property type="entry name" value="SHS2_FTSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003101">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00842">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01174">
        <property type="entry name" value="ftsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FtsA" evidence="6">
        <location>
            <begin position="7"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR003101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="420" mass="47230" checksum="2CD5892D26D124A1" modified="2009-02-10" version="1">MGKWDPVVSIDIGNDSIKGVVVNYSNEGKEVVAYSNLKSKGIESGDIKDVVALNDAMNQIIENLEEQVGKTLKGDFLVSSSVGNFKLQEIREELLLTEGDNLVTVNEKHVDEIKELVLNRALGENNYVYHSYIKKYILDENKIVFNPVDMSAKKLEAIYSIIMGDSIHRSIVDYATRETLGEADYYISPISAAEAVLTSFEKDSGVMHVDLGFYSTVVTIFLNNAPIRFVRLPKSMKYVVLDIAKILKTSIYEAERLLKIYGIAIFQNIEPSIIEYKALDGRTTLETNRELLARIIYARLREIFLNVRKIYRDATIDYKEFRDLGIPGGIVLTGGGAKIPRITDVAADVMKCSVRVGSFINTEEFIIEENEQILSDPQFAAAFGNILQFEKEEGIDTLNKPRNKSSSVFSDFLRKLFKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDH8</accession>
    <name>B7IDH8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">General secretion pathway protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1617</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102390.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1617"/>
    <dbReference type="EnsemblBacteria" id="ACJ76055">
        <property type="protein sequence ID" value="ACJ76055"/>
        <property type="gene ID" value="THA_1617"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1617"/>
    <dbReference type="eggNOG" id="COG2804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013446_10_6_0"/>
    <dbReference type="OMA" id="LIRKPHG"/>
    <dbReference type="OrthoDB" id="749544at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037257">
        <property type="entry name" value="T2SS_E_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007831">
        <property type="entry name" value="T2SS_GspE_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05157">
        <property type="entry name" value="T2SSE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160246">
        <property type="entry name" value="SSF160246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00662">
        <property type="entry name" value="T2SP_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="2">
        <location>
            <begin position="380"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="565" mass="63791" checksum="0A5433D64A27B9F1" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDJ2</accession>
    <name>B7IDJ2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1631</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1631"/>
    <dbReference type="EnsemblBacteria" id="ACJ76069">
        <property type="protein sequence ID" value="ACJ76069"/>
        <property type="gene ID" value="THA_1631"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1631"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_884946_0_0_0"/>
    <dbReference type="OMA" id="EYYSRIY"/>
    <dbReference type="OrthoDB" id="1443304at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="77"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="315" mass="36696" checksum="E9C5DC7F6BCA58B4" modified="2009-02-10" version="1">MEKKGKFLKFLVAFIIAVLFFSNLIFISYFKFQLNQVYGIDEGIFSNFKSYSAYLLSKIPLVNKFVKYEPLSINEPKKYFEEVLESYKKNIEELIKQSEEKLKEAEKLKQENELLYKTLKSIEDEWKERKIKEEISKVEVVKKVENLDSLVEIFKNGDSKELLPLMNSEKVDVKTLAVVFQKLSPDLRSEMVQSLASVNPTKAASVVNTIYNVEEILKEVNSKIKELEDFYAKLYERESQLISLEGFNKAIKSYILSLTEEEIKAFINQYQESPKIVAYILSNLEPSKSRNILKWLKENNEKLFIEIIKLGGGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDK7</accession>
    <name>B7IDK7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="7" type="primary">coaB</name>
        <name evidence="3" type="synonym">coaBC</name>
        <name evidence="7" type="ordered locus">THA_1646</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>(R)-4'-phosphopantothenate + CTP + L-cysteine = CMP + diphosphate + H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 2/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580324.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1646"/>
    <dbReference type="EnsemblBacteria" id="ACJ76084">
        <property type="protein sequence ID" value="ACJ76084"/>
        <property type="gene ID" value="THA_1646"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1646"/>
    <dbReference type="eggNOG" id="COG0452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033319_0_1_0"/>
    <dbReference type="OMA" id="LDMIVAN"/>
    <dbReference type="OrthoDB" id="1346419at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00353"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="SSF102645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3 4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 4" id="KW-0288">FMN</keyword>
    <keyword evidence="3 4 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="5">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="DFP" evidence="6">
        <location>
            <begin position="181"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
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        <location>
            <begin position="186"/>
            <end position="388"/>
        </location>
    </feature>
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        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="388" mass="43428" checksum="F9D69564FAD9C6D1" modified="2009-02-10" version="1">MNIIVGVTSGIAIYKAVDLVSKLRKNKFEIDIIMTENATKMISPVVFSAVGNCPVYTDTFNVKNGWIIHTELSKKADLFVLAPATANTIAKISSGVADNLLTTTILALPEKTPKILIPTMNTRMYENKLTQENFEKLKKLGWFIVEPDSGHLACGDVGKGRYPENEKIIEAIYYMLDEKRLSGKKVLVTAGPTREEIDPIRYISNYSSGKMGYEIAKISKRMGAYTTLISGPTNLTPPYFIDEFIKVKTAHDMYTQVMNKFIDYDIIIMTAAVADYTPKVKSKEKIKKSENNFILELERTKDILKELGRKKRSNQIIIGFAAETSNIIEYGKQKLISKNADYIISNDANKVMGSDFSSAFLISKDKVIQLEGSKEKVAKDILTNIINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDM8</accession>
    <name>B7IDM8_THEAB</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">THA_1667</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580337.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1667"/>
    <dbReference type="EnsemblBacteria" id="ACJ76105">
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        <property type="gene ID" value="THA_1667"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1667"/>
    <dbReference type="HOGENOM" id="CLU_1895205_0_0_0"/>
    <dbReference type="OMA" id="YYPWSAS"/>
    <dbReference type="OrthoDB" id="2098052at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="134" mass="16029" checksum="060EC96EBD3F69A2" modified="2009-02-10" version="1">MISGLFFWIFPFFLIFFDLAFSDLFLSFILFFFLESANNFSFEKALRYVVFLSLYTMVIFKDFNLINVFLIFLLILIESYRDSFLKIWILPLLEAFIFYSLFLINGSLKVFIISLLISLLMYGYLIKKGIIKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDR7</accession>
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        <name evidence="4" type="ordered locus">THA_1708</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
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        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Thermotogales</taxon>
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        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
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                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1708"/>
    <dbReference type="EnsemblBacteria" id="ACJ76144">
        <property type="protein sequence ID" value="ACJ76144"/>
        <property type="gene ID" value="THA_1708"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_735183_0_0_0"/>
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    <dbReference type="OrthoDB" id="1745764at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="207"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="367" mass="42655" checksum="08705FA3A5BB2425" modified="2009-02-10" version="1">MKRFLILIMFIIILNTLFSFEKKAIEYYQAGLNAYQQSNYEKALYYFEKALVIDPSIEGYDTSLKFKAGISAYMIGNYEKAKAYLSGYTENPFVKELLNDILKNSTSTSEEWSNWIEKYKPIELPASTETTNKKNNSKLILFLITFLSSFVILLIMELRVIKAKKTYPQPLETTKEFEQQLTTKTSGIIFNESEELIPENSYTMDFNELINKELSFLSELFEELEEIENNNEEEIKSDIEDNIENSNPEIIAEKENKEEENVDIDEVIEEIDELEEMVSAQDEKLIKNEVEQSLIEKIKSLEISSSEESSGELPIEIAEEFSSLNIEEIFSKLDEKEELEEGELKLITEKLKEFISTTNQKEEEEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDV7</accession>
    <name>B7IDV7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CheY-P phosphatase CheC</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">THA_1750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
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                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580387.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1750"/>
    <dbReference type="EnsemblBacteria" id="ACJ76184">
        <property type="protein sequence ID" value="ACJ76184"/>
        <property type="gene ID" value="THA_1750"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1750"/>
    <dbReference type="eggNOG" id="COG1776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
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    <dbReference type="OMA" id="LYIETEI"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007597">
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF04509">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="SSF103039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheC" evidence="2">
        <location>
            <begin position="9"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="CheC" evidence="2">
        <location>
            <begin position="107"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="205" mass="22465" checksum="89C92877554D5678" modified="2009-02-10" version="1">MLEKLNDSQLDFLKEIGNIGSGNAATALSTMIGKKVDISVPSTKVVPISQIPFIFENPEEIVCAIKMKMREDVEGEILLILNSNTVKEINKILTGSTLEDITSLDEFSASMLKEIGNIMCGSYVTALSGFTGFFINPDPPELAVDMISAVLAEVSLSVSTTEDYILLIETSIKIEDIDKELTGYLLLLPNEESLEKILKKLGMWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDX1</accession>
    <name>B7IDX1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1767</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580395.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1767"/>
    <dbReference type="EnsemblBacteria" id="ACJ76198">
        <property type="protein sequence ID" value="ACJ76198"/>
        <property type="gene ID" value="THA_1767"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1767"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_0"/>
    <dbReference type="OMA" id="QLWGYPP"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="124"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="217" mass="24796" checksum="DC998020D7336A30" modified="2009-02-10" version="1">MKILVVEDDDKLRRLLELEFKHEKCTVKTCEYGEDAIIEYQEFKPDVVVLDIMLPDIDGTEVAKKIRKLDPSAGIIMLTALSEKKNKLEGFESGADDYVVKPFDFEELFARVKAIARRKNITLNDEIKIKDLIINISKREVIFKGKPINLSKTEFDLLAYLVKNAGRVVSKEEILDAVWGISYEGSENTVEVYINYLRKKLSSDIIKTVRGIGYTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDY5</accession>
    <name>B7IDY5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flagellar hook-associated protein 2</fullName>
            <shortName evidence="5">HAP2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Flagellar cap protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1781</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament. Forms a capping structure, which prevents flagellin subunits (transported through the central channel of the flagellum) from leaking out without polymerization at the distal end.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Homopentamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Bacterial flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the FliD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580406.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1781"/>
    <dbReference type="EnsemblBacteria" id="ACJ76212">
        <property type="protein sequence ID" value="ACJ76212"/>
        <property type="gene ID" value="THA_1781"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1781"/>
    <dbReference type="eggNOG" id="COG1345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015182_0_2_0"/>
    <dbReference type="OMA" id="YLWELGI"/>
    <dbReference type="OrthoDB" id="883503at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009421">
        <property type="term" value="C:bacterial-type flagellum filament cap"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009424">
        <property type="term" value="C:bacterial-type flagellum hook"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010810">
        <property type="entry name" value="Flagellin_hook_IN_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040026">
        <property type="entry name" value="FliD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010809">
        <property type="entry name" value="FliD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003481">
        <property type="entry name" value="FliD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30288">
        <property type="entry name" value="PTHR30288"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07196">
        <property type="entry name" value="Flagellin_IN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07195">
        <property type="entry name" value="FliD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02465">
        <property type="entry name" value="FliD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="FliD_N" evidence="6">
        <location>
            <begin position="28"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="FliD_C" evidence="7">
        <location>
            <begin position="353"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="591" mass="65756" checksum="6354F84E977A8139" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDZ8</accession>
    <name>B7IDZ8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative enoyl-(Acyl-carrier-protein) reductase II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1794</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1794"/>
    <dbReference type="EnsemblBacteria" id="ACJ76225">
        <property type="protein sequence ID" value="ACJ76225"/>
        <property type="gene ID" value="THA_1794"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1794"/>
    <dbReference type="eggNOG" id="COG2070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038732_1_1_0"/>
    <dbReference type="OMA" id="IDDLPSC"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017569">
        <property type="entry name" value="Enoyl_ACP_red-II_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03151">
        <property type="entry name" value="enACPred_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857360" evidence="1">
        <location>
            <begin position="23"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="NMO" evidence="2">
        <location>
            <begin position="4"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="NMO" evidence="2">
        <location>
            <begin position="79"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="310" mass="32839" checksum="DF80323A9A9D4D98" modified="2009-02-10" version="1">MNRICKLFNIKYPILMGGMSWAGTPRLAAAVSNAGGLGIIGAGAMKGEDLQNAIDEVRKHTDKPFGVNIILVSPFADELVEVVLKNKVPVVTFGAGNPTKYINDLKSTGIKVVPVVASENLARLVERTGADAVIAEGMESGGHIGEVTTFVLVNSVSKSVKIPVIAAGGIADGKGMAAAFALGAEGIQMGTRFIASKEAEVHENFKNLIIKSGIRDTIITGASLGHSARVIKTKFAKIVKTLEVESPQDAENILVGSLRKAVQEGNIEQGSFMAGQSVGLIKDIKPVHEIIEEIMEEFNETVRKLGEVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE17</accession>
    <name>B7IE17_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Biotin-[acetyl-CoA-carboxylase] ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580437.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1813"/>
    <dbReference type="EnsemblBacteria" id="ACJ76244">
        <property type="protein sequence ID" value="ACJ76244"/>
        <property type="gene ID" value="THA_1813"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1813"/>
    <dbReference type="eggNOG" id="COG0340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051096_3_1_0"/>
    <dbReference type="OMA" id="VGDCIHL"/>
    <dbReference type="OrthoDB" id="1842956at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin-[acetyl-CoA-carboxylase] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="226" mass="26086" checksum="B7C1153F0E58D525" modified="2009-02-10" version="1">MLERSKLICFEKINSTNTYVKENYKNLDNGTIVLAKVQTQGRGRLGRVWISQEGGLWFSILFKKNLKYPNFYTKLSAITLLSILKNLKISAKIKWPNDIFFKEKKLSGILTEIISKNGKVQAIVVGIGLNVNNETPPEGTSVSKVTEKKFEINLILNLFINKFNIYYKFFRLFPFLLTIAWKKNLIFKRNDIINGYKIKKITSEYLIVEKNGLNKKIKSIHELEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE33</accession>
    <name>B7IE33_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-hydroxy-3-methylbut-2-enyl diphosphate reductase</fullName>
            <shortName evidence="5">HMBPP reductase</shortName>
            <ecNumber evidence="5">1.17.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ispH</name>
        <name evidence="7" type="ordered locus">THA_1829</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24488"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24825"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the IspH family.</text>
    </comment>
    <dbReference type="EC" id="1.17.7.4" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580450.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1829"/>
    <dbReference type="EnsemblBacteria" id="ACJ76260">
        <property type="protein sequence ID" value="ACJ76260"/>
        <property type="gene ID" value="THA_1829"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1829"/>
    <dbReference type="eggNOG" id="COG0539">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0761">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015805_3_1_0"/>
    <dbReference type="OMA" id="SLNEFRY"/>
    <dbReference type="OrthoDB" id="1235756at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00097"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00105"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051745">
        <property type="term" value="F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13944">
        <property type="entry name" value="lytB_ispH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00191">
        <property type="entry name" value="IspH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003451">
        <property type="entry name" value="LytB/IspH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035104">
        <property type="entry name" value="Ribosomal_protein_S1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10724:SF7">
        <property type="entry name" value="PTHR10724:SF7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02401">
        <property type="entry name" value="LYTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00681">
        <property type="entry name" value="RIBOSOMALS1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00216">
        <property type="entry name" value="ispH_lytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="289"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="453"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="539"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="625"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="206"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="811" mass="92462" checksum="86AFA379C0B410B4" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE39</accession>
    <name>B7IE39_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease P protein component</fullName>
            <shortName evidence="6">RNase P protein</shortName>
            <shortName evidence="6">RNaseP protein</shortName>
            <ecNumber evidence="6">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Protein C5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">rnpA</name>
        <name evidence="7" type="ordered locus">THA_1835</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RnpA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102793.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1835"/>
    <dbReference type="EnsemblBacteria" id="ACJ76266">
        <property type="protein sequence ID" value="ACJ76266"/>
        <property type="gene ID" value="THA_1835"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1835"/>
    <dbReference type="eggNOG" id="COG0594">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117179_9_2_0"/>
    <dbReference type="OMA" id="RWVREIF"/>
    <dbReference type="OrthoDB" id="2014742at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00227">
        <property type="entry name" value="RNase_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000100">
        <property type="entry name" value="RNase_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020539">
        <property type="entry name" value="RNase_P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33992">
        <property type="entry name" value="PTHR33992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00825">
        <property type="entry name" value="Ribonuclease_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00188">
        <property type="entry name" value="rnpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00648">
        <property type="entry name" value="RIBONUCLEASE_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="131" mass="15743" checksum="4A90C71D5A7FCAAD" modified="2009-02-10" version="1">METSSIKRFGFKKEERLKLRKDILKVYKNGKSIQSSYFVILYCKNGLDYSKFAFSVKKKFGKAVKRNKVKRWMREIVRTNKMNIPKGYDYLIIVRKMLSKDFEQIQFSQFKEAFLELFERIGNEENNIKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE51</accession>
    <name>B7IE51_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leukotoxin translocation ATP-binding protein LktB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1847</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102817.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1847"/>
    <dbReference type="EnsemblBacteria" id="ACJ76278">
        <property type="protein sequence ID" value="ACJ76278"/>
        <property type="gene ID" value="THA_1847"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1847"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_9_0"/>
    <dbReference type="OMA" id="SSKIEHM"/>
    <dbReference type="OrthoDB" id="1497923at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="247" mass="28354" checksum="041C401D00D183D1" modified="2009-02-10" version="1">MFEKLEFINVNFSYEGRKIINNLNFEIKNGEKISIVGSSGEGKSTLIKLILRYINPDSGHILVNGKPLNSIENWYEIVGVLSQRTHIFNRSIRDNLLIAKHDATDKELYNALEFAGLSDFLKTRTLDTILGEEARNISGGERARISLARLILRNPEFVILDEPLEGVDKLVEKEVINNIKDFVEDKTLILISHRFSILSLTDEFAVLENGEIKEKGKFSEHSDKSLLKKFFKAEQELTEKFRRDEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE52</accession>
    <name>B7IE52_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1848</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580460.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1848"/>
    <dbReference type="EnsemblBacteria" id="ACJ76279">
        <property type="protein sequence ID" value="ACJ76279"/>
        <property type="gene ID" value="THA_1848"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1848"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_874169_0_0_0"/>
    <dbReference type="OMA" id="TWISANK"/>
    <dbReference type="OrthoDB" id="1720248at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="28"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="316" mass="37388" checksum="448C3730DD33E922" modified="2009-02-10" version="1">MKQFYKYFFTYHKLLSKKLMFKKLFIDICYILSSILSIILPIFLGKIVDSFLNLNFSYPFFVFILLYVLYFIFSEISSFFRITFYLVDGPKYLFEKAIFTVLKKSDLEVNPKKEMDRIFSFEHVFEFFYGSFAIFVFILPFLVLFSSLMIFINSWKVGLLMIFNFFLLLFSSNKKVDAESKISEKMNESEYNVTNTLSDLYSGYEEIRNYNSLFFSLKWLKDGYNSLVKAYDLFGKSELKFGLSYELFSAVIIPIVIIFISFEVFKGNLTPGIAIMILRYVENVKNYSEVYINDSDYIAWAASRAKDAYDNYLREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE61</accession>
    <name>B7IE61_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">UDP-N-acetylenolpyruvoylglucosamine reductase</fullName>
            <ecNumber evidence="16">1.3.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">UDP-N-acetylmuramate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16 18" type="primary">murB</name>
        <name evidence="18" type="ordered locus">THA_1862</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 16">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>NADP(+) + UDP-N-acetyl-alpha-D-muramate = H(+) + NADPH + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:12248"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="EC" id="1.3.1.98"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 16">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 16">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 16">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the MurB family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.98" evidence="16"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102850.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1862"/>
    <dbReference type="EnsemblBacteria" id="ACJ76288">
        <property type="protein sequence ID" value="ACJ76288"/>
        <property type="gene ID" value="THA_1862"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1862"/>
    <dbReference type="eggNOG" id="COG0812">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035304_1_1_0"/>
    <dbReference type="OMA" id="MQNIGAY"/>
    <dbReference type="OrthoDB" id="841869at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008762">
        <property type="term" value="F:UDP-N-acetylmuramate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00037">
        <property type="entry name" value="MurB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003170">
        <property type="entry name" value="MurB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011601">
        <property type="entry name" value="MurB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036635">
        <property type="entry name" value="MurB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21071">
        <property type="entry name" value="PTHR21071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02873">
        <property type="entry name" value="MurB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56194">
        <property type="entry name" value="SSF56194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00179">
        <property type="entry name" value="murB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7 16" id="KW-0132">Cell division</keyword>
    <keyword evidence="11 16" id="KW-0133">Cell shape</keyword>
    <keyword evidence="15 16" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0274">FAD</keyword>
    <keyword evidence="8 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 16" id="KW-0521">NADP</keyword>
    <keyword evidence="13 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12 16" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="17">
        <location>
            <begin position="30"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="16">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ACJ76288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="297" mass="33758" checksum="87E055A60A22D3AB" modified="2009-02-10" version="1">MRDKIIEEILKFGNDVYIDEPMKCHVSFRIGGPVSAFVVSPSIEIFIKTFSFLKEIREVKVLGNGTNVLPKDSKMDFFILSTNRLNKIEILEDKIICESGLNLKSLCNITAKNGIAGFENLYGIPGSVGGAVYMNAGAYGSEIADLVLYVEAYDGNKIVRFNVNELKFSYRNSLFREEKDLIILRVAFKKFNDEPDLILEKMREIILKRVEKQPLEFPSAGSVFKRPRKDFYVGSAIEKAGLKGFKIGGAKVSEKHAGFIINYNNATYDDVVNLIKYIKEKIYENYKINLETEIEIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE63</accession>
    <name>B7IE63_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1864</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102855.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1864"/>
    <dbReference type="EnsemblBacteria" id="ACJ76290">
        <property type="protein sequence ID" value="ACJ76290"/>
        <property type="gene ID" value="THA_1864"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1864"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_8_0"/>
    <dbReference type="OMA" id="ITMSRFW"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="140" mass="16334" checksum="75BB816F84CCC092" modified="2009-02-10" version="1">MSTSIESLLREICFRVKVKGREVLKEFPITPAQFDLMQKLYFNGEQTMTDLSKILGIAKSTTTGLVSRLEIDGFVERKRREKDKRVITVKLTKKGEEVIDKVILKRIEFVEEALKDFDESSKEKLKELLSLFNESLKKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE74</accession>
    <name>B7IE74_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative Phosphate transport system protein PhoU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102875.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1875"/>
    <dbReference type="EnsemblBacteria" id="ACJ76301">
        <property type="protein sequence ID" value="ACJ76301"/>
        <property type="gene ID" value="THA_1875"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1875"/>
    <dbReference type="eggNOG" id="COG0704">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078518_3_0_0"/>
    <dbReference type="OMA" id="MVYMMED"/>
    <dbReference type="OrthoDB" id="1648549at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:cellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="P_transport_PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PTHR42930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoU" evidence="1">
        <location>
            <begin position="20"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="1">
        <location>
            <begin position="120"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="233" mass="26945" checksum="46FBD239B24213A2" modified="2009-02-10" version="1">MDWILSENVEELKKMVLKEGWLVESLFNQVINALKERDEKASVEVLKKENEVNLMDLKIREKALIILSTMMPMSKNLRMVTGSFIVSGYFQEIAEKTTDIANMVLELIKEPQLKPLITIPEMAKISIEMLRESMRMFADNNIYGAETICEKDADIDNFYDEIRKELMVYMMEDPKYVKRALILLEISQKIEEIADLATKIVEATSYIITGKQYKCFKDKLHTIDSLEKDGGVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE75</accession>
    <name>B7IE75_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative permease, YjgP/YjgQ family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1876</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580467.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1876"/>
    <dbReference type="EnsemblBacteria" id="ACJ76302">
        <property type="protein sequence ID" value="ACJ76302"/>
        <property type="gene ID" value="THA_1876"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1876"/>
    <dbReference type="eggNOG" id="COG0795">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004541_0_0_0"/>
    <dbReference type="OMA" id="ATWLPNI"/>
    <dbReference type="OrthoDB" id="47611at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005495">
        <property type="entry name" value="LptG/LptF_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33529">
        <property type="entry name" value="PTHR33529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03739">
        <property type="entry name" value="LptF_LptG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="1110" mass="129938" checksum="1530590AE3664059" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE76</accession>
    <name>B7IE76_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">23S rRNA (Uracil-5-)-methyltransferase RumA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rumA</name>
        <name evidence="6" type="ordered locus">THA_1877</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102876.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1877"/>
    <dbReference type="EnsemblBacteria" id="ACJ76303">
        <property type="protein sequence ID" value="ACJ76303"/>
        <property type="gene ID" value="THA_1877"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1877"/>
    <dbReference type="eggNOG" id="COG2265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014689_8_1_0"/>
    <dbReference type="OMA" id="SIIHVMN"/>
    <dbReference type="OrthoDB" id="1421660at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030391">
        <property type="entry name" value="MeTrfase_TrmA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="PTHR11061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01231">
        <property type="entry name" value="TRMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRAM" evidence="5">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="395"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="440" mass="50371" checksum="D786FD9A3B020D29" modified="2009-02-10" version="1">MSEIVYIEKMAYGGSGIGKLPGGKVIFVDGAYPGELVEVDVLSEKSDYAIGKLEKIVEKIPERRNPKCPYFRNCGGCNFLDLKYEKQLEFKKMVFMDQLERIAELKLNDLYIEKSSKEYEYRLKMEYSILNDYSFGFKMKNSHKVVSVKNCIIAPKVFDKVLEKLSLTLSHFKIPIYNFKRKKGILKHVVLRYSPNGEVMLIFVTKSEYFKEGKKIASLIKKEFPFIKSIVHVMNSNDKIVLRGPYKVLNGDGVINYEFSWEKFQVPPTAFFQNNFQVAEKMVEFLTKKLDLNGNEVVLDLFSGLGTFSLRLAALSKFVIAVESSHVSVKAGRANANINGLKNIKFIEADALDYLNQVNEKFDVIVLDPPRTGIGTEAVKKILELSPQKIGYVSCNPTTFARDLKLLLEKYEIEDIRIFDMFPQTYHIESIAILNRKNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE84</accession>
    <name>B7IE84_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1885</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102886.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1885"/>
    <dbReference type="EnsemblBacteria" id="ACJ76311">
        <property type="protein sequence ID" value="ACJ76311"/>
        <property type="gene ID" value="THA_1885"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1885"/>
    <dbReference type="HOGENOM" id="CLU_1383603_0_0_0"/>
    <dbReference type="OMA" id="FFIHDES"/>
    <dbReference type="OrthoDB" id="2126947at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="207" mass="23843" checksum="7F46F58BA934BA5F" modified="2009-02-10" version="1">MLSGCALTLDKIIPEKESNQTINFNVYFEAYYSTSLKDFFEEKLEKSFTLSDLDEITVKLKVNDSDIFTFNLKGKNNNVTPPIVIEKSKDAMVKYDIEVTYTLKRDLIDSTEVVLKDTGLIDDLNLLDFQELNLVFFIHDESGTPTLKPKILYEDKKVIKEGIVEDGKIYYVESNNDFYSILKNDKVNEIYVDADSNFSNVYVFPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEA4</accession>
    <name>B7IEA4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ion-translocating oxidoreductase complex subunit G</fullName>
            <ecNumber evidence="6">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Rnf electron transport complex subunit G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rnfG</name>
        <name evidence="9" type="ordered locus">THA_1905</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RnfG family.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102924.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1905"/>
    <dbReference type="EnsemblBacteria" id="ACJ76331">
        <property type="protein sequence ID" value="ACJ76331"/>
        <property type="gene ID" value="THA_1905"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1905"/>
    <dbReference type="eggNOG" id="COG4659">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1155350_0_0_0"/>
    <dbReference type="OMA" id="VYSPVYK"/>
    <dbReference type="OrthoDB" id="1381933at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00479">
        <property type="entry name" value="RsxG_RnfG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007329">
        <property type="entry name" value="FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010209">
        <property type="entry name" value="Ion_transpt_RnfG/RsxG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36118">
        <property type="entry name" value="PTHR36118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04205">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00900">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01947">
        <property type="entry name" value="rnfG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 6" id="KW-0288">FMN</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="FMN_bind" evidence="8">
        <location>
            <begin position="108"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="modified residue" description="FMN phosphoryl threonine" evidence="6">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="222" mass="24331" checksum="AB46505863D146B6" modified="2009-02-10" version="1">MKDMIKTGLILMIYTLVAGLILGFIYTSTQAAIKEAELKNTIDAVKFVLTENGNLLVKEKVIKEAVLKGSKEEEKEIFKKGTSAVLTPVLNFDTERGKVYVLKGYGIGYGGKVITIASFLVNNKKIDLLSIRVIEYSQETPGLGAKISEETSQKRFYPIPYEGLKNGVKVDKDAGKSNLSPEEAKKIGVVKISDVMTGATITPRAVASTIDAMFEYLQKEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEB2</accession>
    <name>B7IEB2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Maltodextrin glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580479.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1915"/>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76339">
        <property type="protein sequence ID" value="ACJ76339"/>
        <property type="gene ID" value="THA_1915"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1915"/>
    <dbReference type="eggNOG" id="COG0366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006462_6_4_0"/>
    <dbReference type="OMA" id="TPDWVKH"/>
    <dbReference type="OrthoDB" id="148706at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="1">
        <location>
            <begin position="18"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="477" mass="56329" checksum="A14F825116EE4C08" modified="2009-02-10" version="1">MKHLFPIPSWVYDTVIYQIFPDRFFIGKGKTVEDKRHLYEKRGGKIEKWGISPKRSNDGSHVKIFYGGDLWGISEKIEYLKDLGVNAIYLTPIFMAQSNHKYDTMDYFKVDPQFGGLKALKNLINNLHNNNMKLILDGVFNHVGKEHVLFRKALKGSKKYRNMFVFYENHYRGWWGTRSLPELVLEETVVKEYISKILWKYLELGIDGWRLDCGQDLGPEMNRFITAKVKEFSSEKYVVSELWTYPSYWSMVDGIMNYHFRENVISYLNDKNPNCGFYIEKAYKETENIHACWNMLDSHDSERLSTVLRDKFQRKAAVLLQFTYPGVPVIYYGTEIGIDGGNDPECRKTMEWDRKKWDNELREFYKKLIYLRKREPALRYGGFELLSNEPLIFMRKSQMVLDNIIVYVNKNDKEIEMVVNVPDGRILSGTEFVDLLSEEKFYVSGGTLKLYMKGRSFGVLKINNKIVRNYDQYKRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEC3</accession>
    <name>B7IEC3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1927</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102969.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1927"/>
    <dbReference type="EnsemblBacteria" id="ACJ76350">
        <property type="protein sequence ID" value="ACJ76350"/>
        <property type="gene ID" value="THA_1927"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1927"/>
    <dbReference type="eggNOG" id="COG0730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_2_3_0"/>
    <dbReference type="OMA" id="YAYGFIM"/>
    <dbReference type="OrthoDB" id="1445898at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="254" mass="27768" checksum="2777BFD23883C9B0" modified="2009-02-10" version="1">MHLSIYEYLFLFFMIFLAGFVDSIAGGGGLISLPAYLFLGIPSHNALATNKLSSSIGSIFSTFRYAKERYVVFEIGIISAIFSFLGSFFGARLALLISDSYLKVIISVLIVFAGAFMLMNKRKKVISKIPKTSFKKKFFISSFIGFIIGMYDGFFGPGTGTFLIIMYTSFLSFDSISASATAKIVNLSSNISALIAFLINGKVLFHIGLPAAFFGILGNWIGSGIAIKKGDKIIKPIVLVILFLVVFKSLRELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEF2</accession>
    <name>B7IEF2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Crispr-associated helicase Cas3 domain protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">THA_1957</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the CRISPR-associated nuclease Cas3-HD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the central section; belongs to the CRISPR-associated helicase Cas3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580505.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1957"/>
    <dbReference type="EnsemblBacteria" id="ACJ76379">
        <property type="protein sequence ID" value="ACJ76379"/>
        <property type="gene ID" value="THA_1957"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1957"/>
    <dbReference type="eggNOG" id="COG1203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2254">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010123_1_1_0"/>
    <dbReference type="OMA" id="IWITTQL"/>
    <dbReference type="OrthoDB" id="1149839at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035011">
        <property type="entry name" value="Cas3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006483">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038257">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006474">
        <property type="entry name" value="Helicase_Cas3_CRISPR-ass_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031:SF539">
        <property type="entry name" value="PTHR24031:SF539"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01587">
        <property type="entry name" value="cas3_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01596">
        <property type="entry name" value="cas3_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51643">
        <property type="entry name" value="HD_CAS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD Cas3-type" evidence="11">
        <location>
            <begin position="18"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="285"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="484"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ76379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="801" mass="95143" checksum="74498F1CF59CD8CC" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEJ5</accession>
    <name>B7IEJ5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579283.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1"/>
    <dbReference type="EnsemblBacteria" id="ACJ74509">
        <property type="protein sequence ID" value="ACJ74509"/>
        <property type="gene ID" value="THA_1"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1"/>
    <dbReference type="eggNOG" id="ENOG5033W19">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1739641_0_0_0"/>
    <dbReference type="OMA" id="AKMYFLA"/>
    <dbReference type="OrthoDB" id="2156365at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="150" mass="17712" checksum="5872FAE498786B43" modified="2009-02-10" version="1">MESSVLLLRLNEENNNKLNEIMNKLKEITNITKAKMYFLAGAYGYFYNTREKIQNNPIDVTRVEFLERSEYKVQKILLNSIYQSQKENLDENINKYKLFEEYANAGVRKLYDIFTESSEIEEFIESLELEVIRIIEALGISTEENSCKEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-03" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEL8</accession>
    <name>RSMH_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA small subunit methyltransferase H</fullName>
            <ecNumber evidence="1">2.1.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA m(4)C1402 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (cytosine-N(4)-)-methyltransferase RsmH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rsmH</name>
        <name type="synonym">mraW</name>
        <name type="ordered locus">THA_24</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = H(+) + N(4)-methylcytidine(1402) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10287"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74506"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. RsmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.199" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579299.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IEL8"/>
    <dbReference type="SMR" id="B7IEL8"/>
    <dbReference type="STRING" id="484019.THA_24"/>
    <dbReference type="EnsemblBacteria" id="ACJ74532">
        <property type="protein sequence ID" value="ACJ74532"/>
        <property type="gene ID" value="THA_24"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_24"/>
    <dbReference type="eggNOG" id="COG0275">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038422_3_0_0"/>
    <dbReference type="OMA" id="NPAKRTF"/>
    <dbReference type="OrthoDB" id="1272633at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071424">
        <property type="term" value="F:rRNA (cytosine-N4-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01007">
        <property type="entry name" value="16SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002903">
        <property type="entry name" value="RsmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023397">
        <property type="entry name" value="SAM-dep_MeTrfase_MraW_recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265">
        <property type="entry name" value="PTHR11265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01795">
        <property type="entry name" value="Methyltransf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004486">
        <property type="entry name" value="MraW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81799">
        <property type="entry name" value="SSF81799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00006">
        <property type="entry name" value="TIGR00006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000387191" description="Ribosomal RNA small subunit methyltransferase H">
        <location>
            <begin position="1"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="36"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01007"/>
        </source>
    </evidence>
    <sequence length="291" mass="33326" checksum="0618201B756F87BF" modified="2009-02-10" version="1">MRRYEDKHIPVMPKEVIEHLIWKKDGIYVDCTVGEGGHTLLLASISPDIFVIGLDIDSEVLNIAEKNLSSFTNVKLFKSSYVDLPVVLKALNIEKVSGILIDLGISTYQLKAEGRGFTFNRDEPLDMRMNLEQKKTAYDVVNFYSEKELADIIFKYGEERFSRRIARSIVNSRPINSTLELVEAIRKALPPQEIRKRKRHFATKTFQAIRIEVNGELSNIENFLNNVPDLLEIGGRLAVISFHSLEDRLVKHFIKNSNKLRQIVGPIIPSKEEIDENPRARSAKLRIAERI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEM2</accession>
    <name>ADEC_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenine deaminase</fullName>
            <shortName evidence="1">Adenase</shortName>
            <shortName evidence="1">Adenine aminase</shortName>
            <ecNumber evidence="1">3.5.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ade</name>
        <name type="ordered locus">THA_28</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenine + H(+) + H2O = hypoxanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23688"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Adenine deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.2" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103343.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IEM2"/>
    <dbReference type="SMR" id="B7IEM2"/>
    <dbReference type="STRING" id="484019.THA_28"/>
    <dbReference type="EnsemblBacteria" id="ACJ74536">
        <property type="protein sequence ID" value="ACJ74536"/>
        <property type="gene ID" value="THA_28"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_28"/>
    <dbReference type="eggNOG" id="COG1001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027935_0_0_0"/>
    <dbReference type="OMA" id="TDHECFT"/>
    <dbReference type="OrthoDB" id="751534at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000034">
        <property type="term" value="F:adenine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006146">
        <property type="term" value="P:adenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01295">
        <property type="entry name" value="AdeC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01518">
        <property type="entry name" value="Adenine_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006679">
        <property type="entry name" value="Adenine_deam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026912">
        <property type="entry name" value="Adenine_deam_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF2">
        <property type="entry name" value="PTHR11113:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13382">
        <property type="entry name" value="Adenine_deam_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01178">
        <property type="entry name" value="ade"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000146246" description="Adenine deaminase">
        <location>
            <begin position="1"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01518"/>
        </source>
    </evidence>
    <sequence length="574" mass="63466" checksum="B9147A1E7B1C8F42" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEN2</accession>
    <name>B7IEN2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ferrous iron transport protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">feoB</name>
        <name evidence="11" type="ordered locus">THA_38</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probable transporter of a GTP-driven Fe(2+) uptake system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. FeoB GTPase (TC 9.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579311.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_38"/>
    <dbReference type="EnsemblBacteria" id="ACJ74546">
        <property type="protein sequence ID" value="ACJ74546"/>
        <property type="gene ID" value="THA_38"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_38"/>
    <dbReference type="eggNOG" id="COG0370">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013350_3_0_0"/>
    <dbReference type="OMA" id="MEMPDYE"/>
    <dbReference type="OrthoDB" id="304844at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055072">
        <property type="term" value="P:iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01879">
        <property type="entry name" value="FeoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003373">
        <property type="entry name" value="Fe2_transport_prot-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011640">
        <property type="entry name" value="Fe2_transport_prot_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030389">
        <property type="entry name" value="G_FEOB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07664">
        <property type="entry name" value="FeoB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02421">
        <property type="entry name" value="FeoB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00437">
        <property type="entry name" value="feoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51711">
        <property type="entry name" value="G_FEOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0410">Iron transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="404"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="498"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="589"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="620"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="FeoB-type G" evidence="10">
        <location>
            <begin position="2"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ74546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="648" mass="73019" checksum="64777449428AAD65" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEP8</accession>
    <name>B7IEP8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_54</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579318.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_54"/>
    <dbReference type="EnsemblBacteria" id="ACJ74562">
        <property type="protein sequence ID" value="ACJ74562"/>
        <property type="gene ID" value="THA_54"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_54"/>
    <dbReference type="eggNOG" id="COG3629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1106693_0_0_0"/>
    <dbReference type="OMA" id="KRRNPMF"/>
    <dbReference type="OrthoDB" id="1323045at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="254" mass="30155" checksum="F63C30FCE177E8F2" modified="2009-02-10" version="1">MEIRLFGKEYGVYFQSEKIVLKSKKAEHILYYLILNYKRKAFVSDILNVFFEGYDESYSRKNLNTLLYMLRKGLNLTKDSLKIDRNVVYLDVSKLKIDYILFQKLMDKSPSKKTRDEIAKIYKGELLEGLDEEWVVPYRELCEMQILMITQQLKKNDLNLFEKLAELPTKNEIPMELALKLIVLDRKRRNPMFIPLLIKSQSDISSKIRKSDFYVKVSKNTYLILFESGKSDSETLENILKFRFNNDIKIIEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEV7</accession>
    <name>B7IEV7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579356.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_113"/>
    <dbReference type="EnsemblBacteria" id="ACJ74621">
        <property type="protein sequence ID" value="ACJ74621"/>
        <property type="gene ID" value="THA_113"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_113"/>
    <dbReference type="eggNOG" id="COG0546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064956_1_0_0"/>
    <dbReference type="OMA" id="TYENMHF"/>
    <dbReference type="OrthoDB" id="1204073at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="213" mass="25246" checksum="9332B1C3E1496306" modified="2009-02-10" version="1">MYIFVDYDGTLVKNSEEKFMKTYFSILSRKVKLPIEKIFNLVMDSIYEITKIEDPSKNLFERFLESISKKTGKSKDYWYNIFLDFYKNEFDELRKIIKVNKKLTNFIKNSNYKFIFASNPLFPEIAVKKRIDFANLSPENFFYIATMENSHYAKPNPKFFAEILEKIGVAADKCLMIGDTENDKASEKVGIKFINVNNFKPELIKIYFEKYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEW3</accession>
    <name>B7IEW3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphate regulon transcriptional regulatory protein PhoB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_119</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103521.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_119"/>
    <dbReference type="EnsemblBacteria" id="ACJ74627">
        <property type="protein sequence ID" value="ACJ74627"/>
        <property type="gene ID" value="THA_119"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_119"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_3_0"/>
    <dbReference type="OMA" id="KARKYEV"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="3"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="124"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="222" mass="25816" checksum="6012094830AE6AE9" modified="2009-02-10" version="1">MKRILIVEDDLSIRNILKKYLKSHGYEVDEAGSIVDFRKKLYNDKFDLVLLDIMLPDGFSINELPDIKVNFPDLGVIIISARDSDNDKIYGLEIGADDYVAKPFNPREVVARIKSYFRRVSKNNEYIKYGPLEIFCENYVVKLNNNEVEMTAKEFEVLCLLAKNPDYVFSREKILDTVWEDEFISDRVVDVHISNIRNKLGKSWIKTIRGAGYKFNPRGYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEW4</accession>
    <name>B7IEW4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate-specific transport system accessory protein PhoU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">phoU</name>
        <name evidence="3" type="ordered locus">THA_120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the regulation of phosphate uptake.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PhoU family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103524.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_120"/>
    <dbReference type="EnsemblBacteria" id="ACJ74628">
        <property type="protein sequence ID" value="ACJ74628"/>
        <property type="gene ID" value="THA_120"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_120"/>
    <dbReference type="eggNOG" id="COG0704">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078518_3_0_0"/>
    <dbReference type="OMA" id="DAMYNSL"/>
    <dbReference type="OrthoDB" id="1648549at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:cellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="P_transport_PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PTHR42930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003107">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02135">
        <property type="entry name" value="phoU_full"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PhoU" evidence="2">
        <location>
            <begin position="17"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="2">
        <location>
            <begin position="121"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR003107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="222" mass="26290" checksum="C367D3EB76637749" modified="2009-02-10" version="1">MDTIHFEKEYSMLRAYISKMLSLVSQSFENAIEALEFFDVSLAKDVINRDDEIDLLNRQIEEKVYDIIARYNPIGKDLRYIITMIKFSNNLERIADLSCNSAEKVIYFEKNNIDYKMIREVKEMFGIVLKMLHDTFLAFSKKDIELSKKIWFDDKNLDDLEKLIRNKSKDLDNKESIIYNILIARDLERIGDHLTNLCEEIIYIETGKEIKEIINYEKDSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEX1</accession>
    <name>B7IEX1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, putative, Mar family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579363.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_127"/>
    <dbReference type="EnsemblBacteria" id="ACJ74635">
        <property type="protein sequence ID" value="ACJ74635"/>
        <property type="gene ID" value="THA_127"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_127"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_4_0"/>
    <dbReference type="OMA" id="LNEHEMT"/>
    <dbReference type="OrthoDB" id="1979687at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="142" mass="16800" checksum="971D9A1E8A7DB053" modified="2009-02-10" version="1">MNKEDILKTIFSLVVNFVNFFHSNKVFSKMTTNEFYVFVFIALNNNVTMKECSQKLNLSKSTITVIIDKLEQENLVKRIRSRKDRRKIFLILTKKGENIFNKFLEDFNKIVDYTISKIPDNELQIINKGFEYFIKNLGGEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF17</accession>
    <name>B7IF17_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acetylornithine aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579391.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_176"/>
    <dbReference type="EnsemblBacteria" id="ACJ74681">
        <property type="protein sequence ID" value="ACJ74681"/>
        <property type="gene ID" value="THA_176"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_176"/>
    <dbReference type="eggNOG" id="COG4992">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_1_0"/>
    <dbReference type="OMA" id="MVPNYNP"/>
    <dbReference type="OrthoDB" id="572533at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="386" mass="43217" checksum="58673603710F6D47" modified="2009-02-10" version="1">MFISNTYKRLPIKVKNAKGIWIYNESGEKYLDTFSGIGVLSFGHCDEEINEAISMQIKKYTHISNFFINENAIFLAKRLVEETKKDGTVFFANSGTEANEAALKAIKKVKKGIIISFENNFHGRSIGSLSITGFQNLREQFEPLLSNIAFLPFNDSKKLENFIENEGNKISAIFVECVHGSGGLDTVNKDFVEVINKYKDIYDYIVVADEVQAGLGRTGEFYSYQNFNLNPDIVTVAKSLGGGLPLSAAIFTGKYKDVFSVGDHGSTFAPNPVSLAAGKTVINRITKTFLDSVKEKSSYLKNKLLTLKKNYPIIKEIKGIGLMLGIEMNNNNYSEKIIEYGLNEKILLNVVKSNTIRLLPALNITHHEIDEMLYRLEKVIKKLNSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF26</accession>
    <name>B7IF26_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579399.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_185"/>
    <dbReference type="EnsemblBacteria" id="ACJ74690">
        <property type="protein sequence ID" value="ACJ74690"/>
        <property type="gene ID" value="THA_185"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_185"/>
    <dbReference type="eggNOG" id="COG1092">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014042_0_0_0"/>
    <dbReference type="OMA" id="VMDVFDY"/>
    <dbReference type="OrthoDB" id="468283at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041532">
        <property type="entry name" value="RlmI_PUA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17785">
        <property type="entry name" value="PUA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="389" mass="44611" checksum="7EAE3DB0DBE932C1" modified="2009-02-10" version="1">MKVYLKKNIKNRVKNGHPWIYENEIEHIDGTPQNGDIVSVFNHERRFVGKGYINFNSKIRIRILTRLNEEINKSFFEKRIKEALLRRNPKEESFRIIFSEADNLPGIIADKFGDYIVIEINTLGMEKFKNEILDILINTFSPKGIFEKSESSSRIKEGLQESVGWIYKSGPELIPYKVNDILFFADTLGQKTGAFLDQRFNALSLKNYSKDAICLDAFCYTGNFGMHMLKFGAKHVTFLDYSERAIEVVNITAKKNNFKNYDTIIGNAFDILKSYDNNSKIFDIISIDPPAFAKNANSKANAKRGYKEINLRAMKILKNNGILATSSCTQVITEEDFKNIIFSAAVDAGKLARILFRGDQPYDHPYVLNILETKYLKFLLLQLENMKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF28</accession>
    <name>B7IF28_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nudix-family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_187</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103676.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_187"/>
    <dbReference type="EnsemblBacteria" id="ACJ74692">
        <property type="protein sequence ID" value="ACJ74692"/>
        <property type="gene ID" value="THA_187"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_187"/>
    <dbReference type="eggNOG" id="COG0494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040940_5_2_0"/>
    <dbReference type="OMA" id="IWGMTAL"/>
    <dbReference type="OrthoDB" id="1623169at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:CoA pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="CoAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="PTHR12992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="183" mass="21029" checksum="B047FF3224C32FBF" modified="2009-02-10" version="1">MFIGAEEYAVVVPVVNKEYFLFEIRSNLLKVQPGEISFPGGKIEYGETPKSAAIRETVEEIGVRPSIISNLPPVYTPFNIIIHPFIGILESSNLNINKYEVEKTIEVPIEIFKSPKYTYDLKVKVIPPSSFPFHLIPNGKDYKWRSGKYKVMFFEYNDHIIWGMTALIAHEAYKKINEKGEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF35</accession>
    <name>B7IF35_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type cobalt transport system, ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_194</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_194"/>
    <dbReference type="EnsemblBacteria" id="ACJ74699">
        <property type="protein sequence ID" value="ACJ74699"/>
        <property type="gene ID" value="THA_194"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_194"/>
    <dbReference type="eggNOG" id="COG1122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_0"/>
    <dbReference type="OMA" id="FVFQRPA"/>
    <dbReference type="OrthoDB" id="1713578at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="222" mass="25184" checksum="23B242075D7719C8" modified="2009-02-10" version="1">MEVFYTNKKIIGPINIKFKNNELVLLLGHNGSGKTTFLKALAGVVNFKGEIISSDGKPFYMATGYVFQNPETQIVGSTVWEDVIFGLENIGLEREEIEKRALETLKTTGLLNYKDFDPYSLSGGQKQKLAISSILAMRPKFLLLDEPTAMLDKYDRMIIKRLIEQLKKTGIGIILATHHVEIFYDIADRIILMKDGLVKYDGAALEKVLREYYEEGGVKWRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF47</accession>
    <name>B7IF47_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Esterase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579413.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_206"/>
    <dbReference type="EnsemblBacteria" id="ACJ74711">
        <property type="protein sequence ID" value="ACJ74711"/>
        <property type="gene ID" value="THA_206"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_206"/>
    <dbReference type="eggNOG" id="COG1075">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_791668_0_0_0"/>
    <dbReference type="OMA" id="HADLAMR"/>
    <dbReference type="OrthoDB" id="1573721at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="331" mass="37999" checksum="E3A70BF20AE73A30" modified="2009-02-10" version="1">MKRFILVLFVLVVSTALLSIPLYSYKLSGVVVFRSLGIKIKPFFEYDEVEVKDYFLFEPELITVREGRKKLILIHGIAPKEVDEKLGFYKKSMIDSFKEIMPDDVGIYFFLYPSLSVDLQYSSKKLIELTDSFKEIYVYAHSMGGILLRYALQDKEFSRKVKMAIFAGTPHVGSPLAQLIFLKTSILNFFTTPKVELIKRALLLANFFNGYILAPNYKYLIFGKKFPPIPDYVKRVNFVGKLEVSVESIDDILKSNPIYFTGLYFLKYIVDNIYPEDSIFLENDGMVPVFSATQDSDINFIFYGANHADLAMRRDIIEKAAEFFGFIGEGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFB4</accession>
    <name>QUEA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-adenosylmethionine:tRNA ribosyltransferase-isomerase</fullName>
            <ecNumber evidence="1">2.4.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Queuosine biosynthesis protein QueA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queA</name>
        <name type="ordered locus">THA_278</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-aminomethyl-7-carbaguanosine(34) in tRNA + S-adenosyl-L-methionine = adenine + epoxyqueuosine(34) in tRNA + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:32155"/>
            <dbReference type="Rhea" id="RHEA-COMP:10342"/>
            <dbReference type="Rhea" id="RHEA-COMP:10346"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82833"/>
            <dbReference type="ChEBI" id="CHEBI:82834"/>
            <dbReference type="EC" id="2.4.99.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the QueA family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.17" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579465.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFB4"/>
    <dbReference type="SMR" id="B7IFB4"/>
    <dbReference type="STRING" id="484019.THA_278"/>
    <dbReference type="EnsemblBacteria" id="ACJ74778">
        <property type="protein sequence ID" value="ACJ74778"/>
        <property type="gene ID" value="THA_278"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_278"/>
    <dbReference type="eggNOG" id="COG0809">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039110_1_0_0"/>
    <dbReference type="OMA" id="YSYGDGM"/>
    <dbReference type="OrthoDB" id="368001at2"/>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051075">
        <property type="term" value="F:S-adenosylmethionine:tRNA ribosyltransferase-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00113">
        <property type="entry name" value="QueA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003699">
        <property type="entry name" value="QueA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042118">
        <property type="entry name" value="QueA_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042119">
        <property type="entry name" value="QueA_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036100">
        <property type="entry name" value="QueA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30307">
        <property type="entry name" value="PTHR30307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02547">
        <property type="entry name" value="Queuosine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111337">
        <property type="entry name" value="SSF111337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00113">
        <property type="entry name" value="queA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000117538" description="S-adenosylmethionine:tRNA ribosyltransferase-isomerase">
        <location>
            <begin position="1"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00113"/>
        </source>
    </evidence>
    <sequence length="335" mass="38326" checksum="F0E1DF47AFD8E676" modified="2009-02-10" version="1">MKLSDFDYYLPEELIAQTPAEPRDSSRLMVLNRKEKTIEHRIFRDIVEYLKPGDLLVRNITKVIPARLYGVKETGARVEILLLEKISEGVWEALVKPGSKVKKGTKIILSEGVEVICKDYSQQGARILEFNCSDDKLFELGNAPLPPYIKNQKVPFERYQTVYSKETGSVAAPTAGLHFTEELLERLRNKGLEFADLILHVGLGTFRPVKVEDIREHKMHSERYYVPAETVKKIRETRKNKGRIIAVGTTSVRTLETIARLPNQESYHGKTDIFIYPPFEFKLTDAIITNFHLPKSTLLMLVAAFAGKDFILEAYNTAVKMKYRFFSLGDACFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFE1</accession>
    <name>B7IFE1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein GrpE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">HSP-70 cofactor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 6" type="primary">grpE</name>
        <name evidence="6" type="ordered locus">THA_305</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the GrpE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103913.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_305"/>
    <dbReference type="EnsemblBacteria" id="ACJ74805">
        <property type="protein sequence ID" value="ACJ74805"/>
        <property type="gene ID" value="THA_305"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_305"/>
    <dbReference type="eggNOG" id="COG0576">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057217_5_2_0"/>
    <dbReference type="OMA" id="YAYEKIA"/>
    <dbReference type="OrthoDB" id="1906715at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_coiled_coil"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="PTHR21237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="SSF51064"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="SSF58014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="196" mass="23337" checksum="9D9E83CCC01B8724" modified="2009-02-10" version="1">MEKKEMKKEKMIENEKVENVKEDANKEKKEKGKEKDLEKIIDEQSKKIEELENQLKEFENYARILKSQFENYKKDVAREKEQISISTIGRIVEKLVPIIDDFKRAFKNVDDETKKTQFFKGMEIIYKNLFKILEGLGLQEIKVGDKFDPFEHEAVERVEDEEKEEYSIVEIVEDGYKFNGRVLKPVKVKVSIKPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFE5</accession>
    <name>B7IFE5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Crispr-associated ramp protein, Cmr6 family, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579488.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_309"/>
    <dbReference type="EnsemblBacteria" id="ACJ74809">
        <property type="protein sequence ID" value="ACJ74809"/>
        <property type="gene ID" value="THA_309"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_309"/>
    <dbReference type="eggNOG" id="COG1604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053305_2_2_0"/>
    <dbReference type="OMA" id="HFQPYYM"/>
    <dbReference type="OrthoDB" id="1359321at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010172">
        <property type="entry name" value="CRISPR-assoc_prot_TM1791"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39965">
        <property type="entry name" value="PTHR39965"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01898">
        <property type="entry name" value="cas_TM1791_cmr6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="248" mass="28841" checksum="02C68A05E868C1E4" modified="2009-02-10" version="1">MKVGYKEDLGNKNIKSFSLFYDKYVYYNLHKNLSEKKNEDKLKDLDNLLKVFFNNNIYIYENSKILNDVFSKALKQLETSGYQIYEKSFELSSPLLIGSGIPSAYEVGIYLNRNYGLPVIPGQSIKGSFKAFLDEIDENDLIFFGNNENSSEIFFFDAVPEKYKLGIDILNPHFKEYYLSENVPNDVYQPVPINFISVFEGTYVFRYVIVSEKNDDNKNKKISEYFEEFLTIYGIGAKTSMGYGRFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFF1</accession>
    <name>B7IFF1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR-associated endoribonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_315</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579494.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_315"/>
    <dbReference type="EnsemblBacteria" id="ACJ74815">
        <property type="protein sequence ID" value="ACJ74815"/>
        <property type="gene ID" value="THA_315"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_315"/>
    <dbReference type="eggNOG" id="COG1583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089858_2_0_0"/>
    <dbReference type="OMA" id="GWMGEYE"/>
    <dbReference type="OrthoDB" id="908607at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045747">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36984">
        <property type="entry name" value="PTHR36984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01881">
        <property type="entry name" value="Cas_Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005054">
        <property type="entry name" value="PF1131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="site" description="Transition state stabilizer" evidence="3">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005054-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="240" mass="27701" checksum="778A47EB4EDFE1A7" modified="2009-02-10" version="1">MRIYLDLDFEKLELPIHYNHILQSIIINMIDSSGYRKFIHDEGYVLGKRKFKLYTFSRLLGKFQIFGNKIAFFNGARLIISSLDDAMVEYIINYILKKGYINVLGEKIYVKAAVLKSLPSANYLRVSTKSPVVVYSTLFNYENKKETIFYEPDSSKFLSLVYENILKKYTAFYGSISKNNIEIKHVGKNPKRVKVVYKNTVIFGWLTAFDIYGDFELLKVAYDAGIGMKNSMGFGCVELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFF5</accession>
    <name>B7IFF5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_319</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579498.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_319"/>
    <dbReference type="EnsemblBacteria" id="ACJ74819">
        <property type="protein sequence ID" value="ACJ74819"/>
        <property type="gene ID" value="THA_319"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_319"/>
    <dbReference type="eggNOG" id="ENOG502ZTE6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1234510_0_0_0"/>
    <dbReference type="OMA" id="FREYSST"/>
    <dbReference type="OrthoDB" id="2087114at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="224" mass="26762" checksum="4A76985C19DEFBA1" modified="2009-02-10" version="1">MKEIILKYLNNKFEEEEENKLYLLIKKIVTIIIGTHSNYKDTLIDIYGTYDSAVNEIINEIFIKIKNKKEILMSIINNIENIESYISRMILNHIIDIVKSYKSKLSLNKEDDDGNEKIDVINIAEFDDLKSEIIAEYFVEELKSLDNMQLCYYFYKVLYNEEIFYSEKSKDAKYKMVQRLKEKLRNLVEDFGIDQKEFAIAVKIYMSEICDKIRHNNREEKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFH0</accession>
    <name>B7IFH0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-oxoglutarate oxidoreductase, gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">porG</name>
        <name evidence="3" type="ordered locus">THA_334</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579506.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_334"/>
    <dbReference type="EnsemblBacteria" id="ACJ74834">
        <property type="protein sequence ID" value="ACJ74834"/>
        <property type="gene ID" value="THA_334"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_334"/>
    <dbReference type="eggNOG" id="COG1014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_0_1_0"/>
    <dbReference type="OMA" id="EVSWMPS"/>
    <dbReference type="OrthoDB" id="1777574at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="9"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="179" mass="19425" checksum="1E023B7CEBB1FF2F" modified="2009-02-10" version="1">MRFVFAGFGGQGVMLMGQILAYAGMIEGKSVTWMPSYGPEMRGGTANCTVVVEDKEVASPVVDKAEVVVAMNIPSMLKFQSFVTDGGYLFLNESVIDREPERKDGINIYKINCNEIADELGNLKVANMVMLGAVIGATNIVSKESVIKALEQKLTGKKADLIDINIKAIERGMQEANKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFI4</accession>
    <name>B7IFI4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-hydantoinase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579516.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_349"/>
    <dbReference type="EnsemblBacteria" id="ACJ74848">
        <property type="protein sequence ID" value="ACJ74848"/>
        <property type="gene ID" value="THA_349"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_349"/>
    <dbReference type="eggNOG" id="COG0044">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_2_0_0"/>
    <dbReference type="OMA" id="RNFVCSP"/>
    <dbReference type="OrthoDB" id="906155at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="1">
        <location>
            <begin position="49"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="431" mass="48871" checksum="12E3DEEE80229CF4" modified="2009-02-10" version="1">MFDLGIINGKLYLDGQYINANLFIKSGKIVDISTTFQKSKEEINAAGKVVLPGFIDPHVHFELNLGKYTSVDDFESGSISALFGGVTTIIDFLDPISNIEELENAFKTRLFAAKKSLVDYSFHATLGNFEGNINTLINKIKEIGTTSIKIFTAYSSSNRRTNDRRMFELFKISKNYNIPILIHAENEDMIIENVPINFHSKARPEISEISEIIKIAEFLNQIKATAYIVHTTCGSSIEEVKNRFNDILNKNIFFESCPHYFYFDDGVYESKKGYLYTMTPPLRNKKEKEKLKNNIDNIFSIGTDHCSFNSKEKRKRKTGNLPMGIGGIEHSFVLMYSLFGEKIIDKFTINPAKFFGLYPKKGTLLPGADADIVIFNPKYNGKISMSHTKADYDLYLGTQIRGKVEKVIKNGVVTIDQDKLVNKVKGNFIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFJ6</accession>
    <name>B7IFJ6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Iron permease FTR1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_366</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579526.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_366"/>
    <dbReference type="EnsemblBacteria" id="ACJ74860">
        <property type="protein sequence ID" value="ACJ74860"/>
        <property type="gene ID" value="THA_366"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_366"/>
    <dbReference type="eggNOG" id="COG0672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077905_0_0_0"/>
    <dbReference type="OMA" id="WYGTLLK"/>
    <dbReference type="OrthoDB" id="2018349at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004923">
        <property type="entry name" value="FTR1/Fip1/EfeU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632">
        <property type="entry name" value="PTHR31632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03239">
        <property type="entry name" value="FTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="258" mass="29034" checksum="39D945F8B3889EA2" modified="2009-02-10" version="1">MASFIITFREALEAVLIVSIIFAFLNKNKLPNYKKHVYLGITLGIVSSIIFGMILATLEEHFAEAFEGIFSLIGAFLITTLILWLHKNKNFNKEIQNKLNSSLNKNFGITLILLVTVNILREGFETVIFLSAIPGDASIIYAILGIVVALVLGYLLYIGILKVNISIFFTVTNVLLILFAAGLIAYGIHELQEAGWFPIIIEHIYDINSFINEKGTFGGFLKTLFGYNGNPSLIEFIAYWTYLIPSMFFAFRKKEIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFQ7</accession>
    <name>B7IFQ7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-lysine 5,6-aminomutase alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579573.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_428"/>
    <dbReference type="EnsemblBacteria" id="ACJ74921">
        <property type="protein sequence ID" value="ACJ74921"/>
        <property type="gene ID" value="THA_428"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_428"/>
    <dbReference type="eggNOG" id="COG0274">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_517447_0_0_0"/>
    <dbReference type="OMA" id="SGLCMPE"/>
    <dbReference type="OrthoDB" id="346908at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015130">
        <property type="entry name" value="Lys-AminoMut_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037086">
        <property type="entry name" value="Lys-AminoMut_asu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09043">
        <property type="entry name" value="Lys-AminoMut_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="SSF51703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lys-AminoMut_A" evidence="1">
        <location>
            <begin position="5"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="518" mass="58071" checksum="723C30BCD54B74FC" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFT3</accession>
    <name>B7IFT3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104223.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_456"/>
    <dbReference type="EnsemblBacteria" id="ACJ74947">
        <property type="protein sequence ID" value="ACJ74947"/>
        <property type="gene ID" value="THA_456"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_456"/>
    <dbReference type="HOGENOM" id="CLU_1937143_0_0_0"/>
    <dbReference type="OMA" id="YFHILID"/>
    <dbReference type="OrthoDB" id="2177231at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="132" mass="16130" checksum="A7178991E08162FB" modified="2009-02-10" version="1">MKNEKYKLIVFLEFLIVYVFSSFILRVNYFTLSPFVVKFFIFYFLVLSLFSSIKGKQFLFSEISRLWIYSFTPLLCYLFFTIGIYFHILIDSRFSYFIPFLFSNYYNLEYDSRKYITLLRLFSFSLFILGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFU0</accession>
    <name>B7IFU0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_463</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104243.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_463"/>
    <dbReference type="EnsemblBacteria" id="ACJ74954">
        <property type="protein sequence ID" value="ACJ74954"/>
        <property type="gene ID" value="THA_463"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_463"/>
    <dbReference type="eggNOG" id="COG1550">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149981_3_0_0"/>
    <dbReference type="OMA" id="RQDSKDY"/>
    <dbReference type="OrthoDB" id="1813824at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007546">
        <property type="entry name" value="DUF503"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036746">
        <property type="entry name" value="TT1725-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36441">
        <property type="entry name" value="PTHR36441"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04456">
        <property type="entry name" value="DUF503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103007">
        <property type="entry name" value="SSF103007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="92" mass="10715" checksum="B074E561D8A8F79F" modified="2009-02-10" version="1">MSASYLEITLRIFGIKSLKEKRSIVKRLINDLRSNFNISVVEIADQDSKDFITLGISLVSINKSHAYKSIESIVEYIERYYTVENVYKEVYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFU2</accession>
    <name>B7IFU2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Threonylcarbamoyl-AMP synthase</fullName>
            <shortName evidence="7">TC-AMP synthase</shortName>
            <ecNumber evidence="4 7">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_465</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + hydrogencarbonate + L-threonine = diphosphate + H2O + L-threonylcarbamoyladenylate</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the SUA5 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="4 7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104245.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_465"/>
    <dbReference type="EnsemblBacteria" id="ACJ74956">
        <property type="protein sequence ID" value="ACJ74956"/>
        <property type="gene ID" value="THA_465"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_465"/>
    <dbReference type="eggNOG" id="COG0009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031397_0_1_0"/>
    <dbReference type="OMA" id="GMLKSHY"/>
    <dbReference type="OrthoDB" id="1913202at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038385">
        <property type="entry name" value="Sua5/YwlC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005145">
        <property type="entry name" value="Sua5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010923">
        <property type="entry name" value="T(6)A37_SUA5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03481">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004930">
        <property type="entry name" value="Tln_factor_SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00057">
        <property type="entry name" value="TIGR00057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="9">
        <location>
            <begin position="15"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR004930-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="337" mass="37470" checksum="2BD2BCFE6655D5F0" modified="2009-02-10" version="1">MKTKILKIDPLDFNEKDLIEVCNVIKSGGLVAFPTETVYGLGASAFEEKAVDNIFKVKGRAKDNPLIVHVDRFEKLLEIAEVAEKYFEAINKLTPGPVTFVLKQKINLPKNVTAGLKTVGVRIPAHPVARKLCKCAGPIAAPSANLSGKPSPTDSQAVIEDLFGKIDFIIDAGNSEFGLESTIIDLTSEIPIVLRPGPITIEQLESIFGKVEIPQFVIDASSVENPKAPGMKYRHYAPEKPVYRFNKVQREKVIKKALSENGVFICPEEHKKFYPDDRIMILGKLSEPYSIAQNLFKVLREFDRSNFNVAFIEAFEEKGILLSVMNRLKKASVEVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFV4</accession>
    <name>B7IFV4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Deoxyribose-phosphate aldolase</fullName>
            <shortName evidence="3">DERA</shortName>
            <ecNumber evidence="3">4.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">2-deoxy-D-ribose 5-phosphate aldolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphodeoxyriboaldolase</fullName>
            <shortName evidence="3">Deoxyriboaldolase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">deoC</name>
        <name evidence="4" type="ordered locus">THA_477</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>2-deoxy-D-ribose 5-phosphate = acetaldehyde + D-glyceraldehyde 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12821"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="EC" id="4.1.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; 2-deoxy-D-ribose 1-phosphate degradation; D-glyceraldehyde 3-phosphate and acetaldehyde from 2-deoxy-alpha-D-ribose 1-phosphate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the DeoC/FbaB aldolase family. DeoC type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.4" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579606.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_477"/>
    <dbReference type="EnsemblBacteria" id="ACJ74968">
        <property type="protein sequence ID" value="ACJ74968"/>
        <property type="gene ID" value="THA_477"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_477"/>
    <dbReference type="eggNOG" id="COG0274">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053595_0_1_0"/>
    <dbReference type="OMA" id="KRGIGNE"/>
    <dbReference type="OrthoDB" id="1227369at2"/>
    <dbReference type="UniPathway" id="UPA00002">
        <property type="reaction ID" value="UER00468"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004139">
        <property type="term" value="F:deoxyribose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046386">
        <property type="term" value="P:deoxyribose phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00959">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00114">
        <property type="entry name" value="DeoC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011343">
        <property type="entry name" value="DeoC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028581">
        <property type="entry name" value="DeoC_typeI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889">
        <property type="entry name" value="PTHR10889"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889:SF1">
        <property type="entry name" value="PTHR10889:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001357">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00126">
        <property type="entry name" value="deoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with acetaldehyde" evidence="3">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="240" mass="26344" checksum="A3A4351AD25878B2" modified="2009-02-10" version="1">MDIKKIVEEKIREFKESYKFELKNIEVKDVNKYIEHTNLKATATEEDILKLCEQAKEYNFRGVCVNPSFVPLVKKQLEGTDVKVVTVIGFPLGATSTRAKVEETKVAVADGADEVDMVIHIGYLKQKNYEYIYNDIKAVVEAANVPVKVIIETCYLTDEEKVAACVISKEASAEFVKTSTGFGTAGAKVEDVNLMRWVVGDSMGVKASGGVKTYEDAVNMIRAGANSIGTSSGIDIVKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFV6</accession>
    <name>B7IFV6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C</fullName>
            <shortName evidence="1">Asp/Glu-ADT subunit C</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">gatC</name>
        <name evidence="2" type="ordered locus">THA_479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579607.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_479"/>
    <dbReference type="EnsemblBacteria" id="ACJ74970">
        <property type="protein sequence ID" value="ACJ74970"/>
        <property type="gene ID" value="THA_479"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_479"/>
    <dbReference type="eggNOG" id="COG0721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105899_4_1_0"/>
    <dbReference type="OMA" id="ESTEPMY"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00122">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="Asp/Glu-ADT_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004">
        <property type="entry name" value="PTHR15004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="Glu-tRNAGln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="SSF141000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00135">
        <property type="entry name" value="gatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="93" mass="10860" checksum="9F3C6447882F85D1" modified="2009-02-10" version="1">MKIDEKLIEEIAKLARLEIENKQEFINEMQKIVDYFELLNNVDTECIEPMYTPLESSASLRENGAVDFENVEGIRENFPEREGNYLKVPGIHK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG41</accession>
    <name>ATPD_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F(1) sector subunit delta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-type ATPase subunit delta</fullName>
            <shortName evidence="1">F-ATPase subunit delta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpH</name>
        <name type="ordered locus">THA_568</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">This protein is part of the stalk that links CF(0) to CF(1). It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase delta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579653.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IG41"/>
    <dbReference type="SMR" id="B7IG41"/>
    <dbReference type="STRING" id="484019.THA_568"/>
    <dbReference type="EnsemblBacteria" id="ACJ75055">
        <property type="protein sequence ID" value="ACJ75055"/>
        <property type="gene ID" value="THA_568"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_568"/>
    <dbReference type="eggNOG" id="COG0712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085114_4_0_0"/>
    <dbReference type="OMA" id="YESFATF"/>
    <dbReference type="OrthoDB" id="1937493at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="PTHR11910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="SSF47928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000382159" description="ATP synthase subunit delta">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01416"/>
        </source>
    </evidence>
    <sequence length="183" mass="20992" checksum="8CD9487B487D119A" modified="2009-02-10" version="1">MRYSVIASKYVKALLIVGQKLNKIEKYGEFLSFVKNIYESFATFFNNPIVKPEQKTLVIKQAFEEVFKESPGEAFLNFINIVFENKREKFIPQMQALYKYAAIDIENKILVNVKTAVKLSDQEIKVINDFVEKYVGKTPVIEETIDESLIAGAVIEFAGKMIDVSIKGRMDKIAKEVFFLRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG64</accession>
    <name>B7IG64_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100515.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_591"/>
    <dbReference type="EnsemblBacteria" id="ACJ75078">
        <property type="protein sequence ID" value="ACJ75078"/>
        <property type="gene ID" value="THA_591"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_591"/>
    <dbReference type="eggNOG" id="ENOG502ZUSM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_201756_0_0_0"/>
    <dbReference type="OMA" id="VVEFENR"/>
    <dbReference type="OrthoDB" id="2064911at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="64" mass="7750" checksum="1BF83C24FD66CE61" modified="2009-02-10" version="1">MENNTLEELVRRYLKVKETIKELNREKKELEEMIVEFVEHMDIDNIIVDGVMVEFARKTKIQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG65</accession>
    <name>B7IG65_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Xanthine/uracil permease family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_592</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579666.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_592"/>
    <dbReference type="EnsemblBacteria" id="ACJ75079">
        <property type="protein sequence ID" value="ACJ75079"/>
        <property type="gene ID" value="THA_592"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_592"/>
    <dbReference type="eggNOG" id="COG2252">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024508_0_1_0"/>
    <dbReference type="OMA" id="YVDIIDC"/>
    <dbReference type="OrthoDB" id="558559at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904823">
        <property type="term" value="P:purine nucleobase transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026033">
        <property type="entry name" value="Azg-like_bact_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="PTHR43337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005353">
        <property type="entry name" value="PbuG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="446" mass="47491" checksum="0248A5CDD2A36847" modified="2009-02-10" version="1">MEKFFKLKESGSSVRTEIIAGITTFLTMAYIIFVNPNILINVIPGATPDSPLYAQFFGAFMVATILGAATATLIMGLWANYPFALAPGMGLNAYFAFTVCGKLGIDWRVALAAVFVEGILFILLAVSGVRGFVVKAIPNSIKLATSAGIGLFIAFIGLKSAGIVVADGVTYVTLGDLTSPTALVTIIGFFIIAILFALRVPGSILIGILASTFIGMIPVFNVTNFQGVVGKIPDISPTFFKLFEKFSWADLASGTFWIVVFTFFFVDFFDTLGTLTGLAESAGFIKNGEFPRANRAYLSDAVGTTVGALFGTSTVTTYIESSTGIAEGGRTGLTAVVVAILMLLMLFFAPLGMSIPAAATAPALIFVGALMLKGLKGVNWDDITEALPAFITMIIMPLTYSIANGIALGLIVYPIVKTFSGKTKDVHWLNWILAFLFVLYLIYLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG89</accession>
    <name>B7IG89_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycerol-3-phosphate ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_617</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579685.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_617"/>
    <dbReference type="EnsemblBacteria" id="ACJ75103">
        <property type="protein sequence ID" value="ACJ75103"/>
        <property type="gene ID" value="THA_617"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_617"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_3_1_0"/>
    <dbReference type="OMA" id="ESFSAWH"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="437" mass="49544" checksum="258BABF10D280663" modified="2009-02-10" version="1">MKKLLVLILVVISILSFSKVTIEFWHAMSGWRIELLENMAKEFMATHPDIQVNVQYTGSYRDTFNKTIAAVKAGNAPHVVQIYDIGTRAMIDGNIAVPIGDLIAKDPSIDLGAFLPQVLNYYTVNGKLYSMPFNSSNAILFYNKTFFKEAGLDPNRPPRTFDELIEYSRKLVKKDKDGNIIRYGLTWPTHSWFFEQLMAVQDEPLVNYDNGRGDKRPTEAVFNSQAGKNIFELLAKMTKEGLLINTKREDWSGARQIFLSGKAAMLLYSTSDVKFITDTAKENGWEVGTAFLPKPSLSIQGGVVIGGGSLWILKNHPKEEIDAAWEFVKWMTEPAQQIKWHLETGYFPVRKDALEQLLMEGFYADHPNYLTAIFQLLLSKQTPNTNGAIIGVFPETREIIETAYEKVINGEMTVDQALDWAADQVTRALKKYNRLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGB1</accession>
    <name>B7IGB1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein HflK</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hflK</name>
        <name evidence="7" type="ordered locus">THA_647</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">HflC and HflK could encode or regulate a protease.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">HflC and HflK may interact to form a multimeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the band 7/mec-2 family. HflK subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104484.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_647"/>
    <dbReference type="EnsemblBacteria" id="ACJ75125">
        <property type="protein sequence ID" value="ACJ75125"/>
        <property type="gene ID" value="THA_647"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_647"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039173_0_1_0"/>
    <dbReference type="OMA" id="WNEPGGN"/>
    <dbReference type="OrthoDB" id="577287at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03404">
        <property type="entry name" value="SPFH_HflK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010201">
        <property type="entry name" value="HflK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_HflKC_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327:SF2">
        <property type="entry name" value="PTHR43327:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01933">
        <property type="entry name" value="hflK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="PHB" evidence="6">
        <location>
            <begin position="21"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="309" mass="34865" checksum="60B2440D8950C714" modified="2009-02-10" version="1">MWKKLIGWLVLAIIILIYLSIGVYQVGPSEVALIKTFGKYTHSTGPGIHFHLPYPFQSHVIVDVETIRKEEIGFRTIESYGKISYRTVNEEALMLTGDGNIISVEAAVQYRIKDPVKFAFNVINGKELVRFTTESVLRERIAVRTIDDVLTVERDKIALETAEKVQEILDSYDSGILINKVYLQEVAPPDQVVAAFDDVNNAKQDKERFINEATKYANDVIPKAQGQAEKILREAEAYAQKKILEAQGETQRFLSVLKEYEIAPEITKKRLILEKLQSVFSSTKNIFVLDDSGTIKLLNVNDLIGGDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGB9</accession>
    <name>B7IGB9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_656</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579704.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75133">
        <property type="protein sequence ID" value="ACJ75133"/>
        <property type="gene ID" value="THA_656"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_656"/>
    <dbReference type="HOGENOM" id="CLU_1659882_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="159" mass="18724" checksum="B3BDFFD05B51A481" modified="2009-02-10" version="1">MNKELHKIQKKEPILIINDNDYREFCNEKARKVINFYKNHSEYNKIIIFPGFHPEEFGLPASSLLLSDKIYPFSTGFDVGCGYLILIVYDIDPIKMNKITKKLENSNEISKIFNKNIENSSWIIKKMNKITKNIIRLQLTKPGKYQLEILAYTVKIFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGD1</accession>
    <name>B7IGD1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribose import ATP-binding protein RbsA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_670</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579714.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_670"/>
    <dbReference type="EnsemblBacteria" id="ACJ75145">
        <property type="protein sequence ID" value="ACJ75145"/>
        <property type="gene ID" value="THA_670"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_670"/>
    <dbReference type="eggNOG" id="COG1129">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_92_3_0"/>
    <dbReference type="OMA" id="GFGFCPE"/>
    <dbReference type="OrthoDB" id="551294at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="249"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="492" mass="55506" checksum="16FC1D7E4351F899" modified="2009-02-10" version="1">MEKILYMKDIIKTFPGVIALNNVKFDLYKGEVHALLGENGAGKSTLIKIISGVYKADSGEIYINGRKSFFSNPTEAIQQGIATIHQELNLFEELTVAENIFVGNINKFNISKSKMIRIVDNLLNEFNFPIKASEKVKKLNTSEKQLVSIAKALSLNAKIIIMDEPTATITEHETRRLFEIIKELKSKNISVIFISHRLEEIFEIADRVTVLRDGNYIGTTHIEDVSKEKLIKMMVGRDINEMYPKFNKISEEEIFKIENLNVGNFVKNVSFNVKKGEIFGIAGLVGSGKLEIALGIYGYLPSKWKKLVLNGEELLPERNMFRHGIALVPEDRKKMGLVLEMDVMKNIILQNTDEVTKFGKISWKKSRRVANEEIRKYKIKVSSINQKVKTLSGGNQQKVVLSKVLRKKPKIIFLVEPTRGIDVGAKVEVYNIINKLANIGVGVVFISSELPEIVSLCDRVLVMHRGESMGILDKDEINQENILKLATGVDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGE3</accession>
    <name>B7IGE3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isoaspartyl dipeptidase</fullName>
            <ecNumber evidence="1">3.4.19.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">iadA</name>
        <name evidence="6" type="ordered locus">THA_685</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolytic cleavage of a subset of L-isoaspartyl (L-beta-aspartyl) dipeptides. Used to degrade proteins damaged by L-isoaspartyl residues formation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 4">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Carboxylation allows a single lysine to coordinate two zinc ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M38 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.-" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579726.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_685"/>
    <dbReference type="EnsemblBacteria" id="ACJ75157">
        <property type="protein sequence ID" value="ACJ75157"/>
        <property type="gene ID" value="THA_685"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_685"/>
    <dbReference type="eggNOG" id="COG1228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058216_0_0_0"/>
    <dbReference type="OMA" id="HVHICGG"/>
    <dbReference type="OrthoDB" id="1319925at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008798">
        <property type="term" value="F:beta-aspartyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010229">
        <property type="entry name" value="Pept_M38_dipep"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001238">
        <property type="entry name" value="IadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01975">
        <property type="entry name" value="isoAsp_dipep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="5">
        <location>
            <begin position="49"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="65"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR001238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001238-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001238-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001238-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="365" mass="39919" checksum="BED5CDA83FB06C7F" modified="2009-02-10" version="1">MLTLLKNLKVFTPYYIGKKDILIANGKIELIEENLVVPFSKVLDLNGKIAVPGFIDAHVHIIGGGGEGGFQTRTPEITIEELVDSGITTVIGCLGTDDVTRSMEDLYAKSKQLEELGITSFIYTGSYSVPVKTITGDIKKDLVLIDKVIGVGEIAISDHRSSQPTLEEIKRIVAQARVGGLISNKAGVVNFHVGNGKNGIDYLFEIAENTEIPIYHMYPTHVGRSKELFHRAIEFAKKGGFIDLTASESVVDYLEIAINEGVIDNLTMTSDGHGSLPKFDENGRLIKLDVASTTVLFESVKKAVERDLNFEEVLKTITINPAKVLKLKSKGRIEKETDGDIVVLDGNLNIEKVIARGKLLKEVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGI1</accession>
    <name>B7IGI1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">High-affinity zinc uptake system ATP-binding protein ZnuC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_727</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_727"/>
    <dbReference type="EnsemblBacteria" id="ACJ75195">
        <property type="protein sequence ID" value="ACJ75195"/>
        <property type="gene ID" value="THA_727"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_727"/>
    <dbReference type="eggNOG" id="COG1121">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_0"/>
    <dbReference type="OMA" id="GHDHVHP"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="8"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="242" mass="27429" checksum="F7D83C64632E5930" modified="2009-02-10" version="1">MMANDIAVIVKDLNYLIGEKYILKNINFSMKKGEFVGIVGPNGAGKSTLIKILIGEIDNYVGEVMVRGKIGYLPQVQTFNREVPINAYQFVKMGSYKNKKNVKLIEKLIEEVGLSGKEKQLIGTMSGGEIQRLSLARALVSEPDILILDEPEAGVDQMGKARFYELLEEFQKRLNLTIIMVSHDIGMVFEKCTTVMCLNKTLHCHGPTEKIKPDELKLLFPDFDLWIRSKNHYEKEHEHGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGL2</accession>
    <name>B7IGL2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579784.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_761"/>
    <dbReference type="EnsemblBacteria" id="ACJ75226">
        <property type="protein sequence ID" value="ACJ75226"/>
        <property type="gene ID" value="THA_761"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_761"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_0"/>
    <dbReference type="OMA" id="SVAMTGW"/>
    <dbReference type="OrthoDB" id="554560at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="27"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="373" mass="41956" checksum="3D9F9755596E249B" modified="2009-02-10" version="1">MNIIDEIPSSKTLEINALASKLKSEGKDVINLTAGEPDFPTPDVIKQKAHEALDINFTRYTDSKGIVQLRETISKIMQQKGFNFSKDEIIVTNGGKQALFNAVLSTVDKDEEVILISPYWVSYPPMIMLAGASIKVLNTTFEEGFVPNIEELKALISDKTKAIIINSPNNPTGVIYPEEVLRKIAEIANKNKIFVLADDVYDSLVYDGKYTSIANFVDPEYLIYINAFSKSHAMTGWRVGYIATKNKKIYKRIAKIQAHTTSSVNSIAQYAASFALEADTSYMFEEFKKRRDFTIEMAQKIGLEFVKPNGAFYLFFKSPMENDEEFCKKLLEEKLVALVPGSAFMAPGFVRMSFANSLEAINEAFNRIKEFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGM7</accession>
    <name>B7IGM7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_779"/>
    <dbReference type="EnsemblBacteria" id="ACJ75241">
        <property type="protein sequence ID" value="ACJ75241"/>
        <property type="gene ID" value="THA_779"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_779"/>
    <dbReference type="eggNOG" id="COG1668">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064505_0_0_0"/>
    <dbReference type="OMA" id="SLVMRFI"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="320" mass="36540" checksum="6027598BFD7821FA" modified="2009-02-10" version="1">MRVWYEFKRLLGKPLNIILIILLPVVLTIASIIFFNGFGVTSIKLGVYNLDNSPLSKFTIKLVMSFFKGGTLTYVDGDYSRFLQNGELNAVMVIPENFTDALYKGEKVNIDFIPSPVDLQLSVGIYNVLNSVFNDLSGSPFFNPQVLRYLFVSNETPAPSFVPKTKNFKTDFGYLFSPAILFLSVIFIILSIGVLTVVNDRDLGLISIFKVNNEKWYVYGLNKFLALFFLGLFVSIAVYIAGIFFGNKDRTIYILTFIHCWNSISRITCINNVLSFSKQGNFEYIWLFSVNVFLFYKRKYNASHNTSKIHVQTFQFHSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGM9</accession>
    <name>B7IGM9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_781</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100347.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_781"/>
    <dbReference type="EnsemblBacteria" id="ACJ75243">
        <property type="protein sequence ID" value="ACJ75243"/>
        <property type="gene ID" value="THA_781"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_781"/>
    <dbReference type="eggNOG" id="ENOG502ZIT1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1309602_0_0_0"/>
    <dbReference type="OMA" id="FNTEDIF"/>
    <dbReference type="OrthoDB" id="1835244at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="210" mass="24693" checksum="0E939CFD90775A7F" modified="2009-02-10" version="1">MKKLITIILISISIFSFSMKFGFSTSQGVWFSEKIENFRIRIGYPYSQFGLIVPSEFLDYGINIGYLLNNSNSLLEVFFDTSINNFYFKSFVKTSFHAPENYDETTETGRIYVGMAPAYYFTKNIRLDFNFEYSAVYLYYDPGSETGFPSIFNTEDIFRYNIELLVSYFMKDIRIFLGMSFKYRWIDYLSLPLFNDQLVSFGIEVEVNGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGU5</accession>
    <name>B7IGU5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_848</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579829.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_848"/>
    <dbReference type="EnsemblBacteria" id="ACJ75309">
        <property type="protein sequence ID" value="ACJ75309"/>
        <property type="gene ID" value="THA_848"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_848"/>
    <dbReference type="eggNOG" id="ENOG5033TFG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014912_0_0_0"/>
    <dbReference type="OMA" id="VIPYGFI"/>
    <dbReference type="OrthoDB" id="456205at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024952">
        <property type="entry name" value="Lipo_LPP20-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02169">
        <property type="entry name" value="LPP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002854525" evidence="1">
        <location>
            <begin position="21"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="682" mass="76753" checksum="DF8526D805A92DA3" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGV4</accession>
    <name>B7IGV4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Crispr-associated ramp protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_858</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579838.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_858"/>
    <dbReference type="EnsemblBacteria" id="ACJ75318">
        <property type="protein sequence ID" value="ACJ75318"/>
        <property type="gene ID" value="THA_858"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_858"/>
    <dbReference type="eggNOG" id="COG1604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057129_1_0_0"/>
    <dbReference type="OMA" id="FYPTYFD"/>
    <dbReference type="OrthoDB" id="1171756at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="333" mass="39207" checksum="25E8A97F7616B2FB" modified="2009-02-10" version="1">MKKPIKFETYINIKNKMIEYNGLIISNNTLNIIEKAKKISGILKENELENYISRLIPYSFIFWAEIELINPYFSKDDEDYNLTKKNLCIKDKAFKIPMIRGSAWKGVFANAFKILINESLFSSNTEEKAIDYIQSFARIFGTGSMEFRKFTKMLSELLEDKLKEKDMHEIIKYLIFELGLRLKKDDIDNLRKKENFKDWVRNNIWKKYGETSEKNLPFFLKPHKGRAIFYPTFFSRLSTEVINPHSKEERKGKGPIFFEVVSKYSRGIVQIVYIPFDGIRLEREVLKDQVEKDVKFIELSFKKVSDIGIGAKSKLGWGRFTIKSKKLKFAAMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH47</accession>
    <name>B7IH47_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Indolepyruvate oxidoreductase subunit IorB</fullName>
            <ecNumber evidence="3">1.2.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Indolepyruvate ferredoxin oxidoreductase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">iorB</name>
        <name evidence="9" type="ordered locus">THA_952</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>CoA + indole-3-pyruvate + 2 oxidized [2Fe-2S]-[ferredoxin] = (indol-3-yl)acetyl-CoA + CO2 + H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:12645"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57271"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="EC" id="1.2.7.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of the IorA and IorB subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.8" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100895.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_952"/>
    <dbReference type="EnsemblBacteria" id="ACJ75411">
        <property type="protein sequence ID" value="ACJ75411"/>
        <property type="gene ID" value="THA_952"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_952"/>
    <dbReference type="eggNOG" id="COG1014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_1_1_0"/>
    <dbReference type="OMA" id="HGMSQRF"/>
    <dbReference type="OrthoDB" id="1603697at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043805">
        <property type="term" value="F:indolepyruvate ferredoxin oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017719">
        <property type="entry name" value="Indolepyruvate_Fd_OxRdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03334">
        <property type="entry name" value="IOR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="8">
        <location>
            <begin position="15"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="204" mass="22125" checksum="84E8257946850604" modified="2009-02-10" version="1">MVAVKEYNIVITGVGGQGILTAANLLGWAALNEGYKVRVGEVHGMSQRFGSVISYVRFGENVYGSMVPEGKADVILSFEPVEALRYVNYLKEGGLVFTNSRPIQPVQVAMGLASYPSHEQIKDIVENKFNAKYISFDAESIAKDAGNIIATNVVLIGALTQTPDFPLSPESIKEVIKVSVPKKAVDLNMKAFDMGIKTAQELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH94</accession>
    <name>B7IH94_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_999</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579920.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_999"/>
    <dbReference type="EnsemblBacteria" id="ACJ75458">
        <property type="protein sequence ID" value="ACJ75458"/>
        <property type="gene ID" value="THA_999"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_999"/>
    <dbReference type="eggNOG" id="COG1801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046519_0_1_0"/>
    <dbReference type="OMA" id="VLFNNCC"/>
    <dbReference type="OrthoDB" id="1068600at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002763">
        <property type="entry name" value="DUF72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036520">
        <property type="entry name" value="UPF0759_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348">
        <property type="entry name" value="PTHR30348"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01904">
        <property type="entry name" value="DUF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117396">
        <property type="entry name" value="SSF117396"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="249" mass="30245" checksum="BAACCC43DA908949" modified="2009-02-10" version="1">MIYVGTSGFQFDDWIGKVYDPNIKKNEMLKYYIGKYKFNTVELNYTYYKMPGFRTIVSLLRNTPRNFYFSIKLYGKITHEHDLSYVDKFLDATKPMVEEKRLIGYLAQFPFSFKRTDENERFLYKISRKFNNLFVELRHISWIDFNTDDFEIVTIDQPPLKDFLPFVVKAKERLYVRLHGRNKMWFEEDAKKRYNYKYSRQELVKIYKDIKDFEGPKYVYFNNCYEGKALLDALEFREISGGEILEFFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHA8</accession>
    <name>TRPB_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="1">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpB</name>
        <name type="ordered locus">THA_1014</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579932.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHA8"/>
    <dbReference type="SMR" id="B7IHA8"/>
    <dbReference type="STRING" id="484019.THA_1014"/>
    <dbReference type="PRIDE" id="B7IHA8"/>
    <dbReference type="EnsemblBacteria" id="ACJ75472">
        <property type="protein sequence ID" value="ACJ75472"/>
        <property type="gene ID" value="THA_1014"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1014"/>
    <dbReference type="eggNOG" id="COG1350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042858_1_0_0"/>
    <dbReference type="OMA" id="MLHQTII"/>
    <dbReference type="OrthoDB" id="912282at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006316">
        <property type="entry name" value="Trp_synth_b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF6">
        <property type="entry name" value="PTHR48077:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500824">
        <property type="entry name" value="TrpB_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01415">
        <property type="entry name" value="trpB_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_1000117764" description="Tryptophan synthase beta chain">
        <location>
            <begin position="1"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <sequence length="420" mass="46788" checksum="84694C3AC50AA6E8" modified="2009-02-10" version="1">MREFVNLKFEEMPKVWYNVLSDLPFKLDPPLDPETNKPMSPEKLLKIFPAPLLEQEVNDTDKFIDIPEEILKEYAVYRPTPLIRANFLEEYLQTPAKIYYKYEGQSPTGSHKTNTALAQAYYNKISGVKKLYTETGAGQWGSALSYSGLKFGINVNIYMVRVSFNQKPARKSIMKLFNGKVTPSPSRNTKSGRKYEENHPGSLGIAISEAMEEVLQRNDSKYALGSVLNHVLLHQTIIGLEIKKQLEKLNIQPDVIIGCHGGGSNFGGTILPFIPDKLSGKNIRFIACEPESCPTLTKGEYRYDFGDTAGFTPLLKMYTLGKDFIPPSIHAGGLRYHGAAPIISALLNHNLIEAKAFSQEETFKAARLFSKLEGIIPAPESSHAIAGAIKEALNAKKENKEKIIVFTLSGHGLFDLNAYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHC1</accession>
    <name>B7IHC1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Phosphoglucomutase/phosphomannomutase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">THA_1027</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579942.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1027"/>
    <dbReference type="EnsemblBacteria" id="ACJ75485">
        <property type="protein sequence ID" value="ACJ75485"/>
        <property type="gene ID" value="THA_1027"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1027"/>
    <dbReference type="eggNOG" id="COG1109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_0_0_0"/>
    <dbReference type="OMA" id="PQDNGYK"/>
    <dbReference type="OrthoDB" id="637615at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016868">
        <property type="term" value="F:intramolecular transferase activity, phosphotransferases"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="8">
        <location>
            <begin position="43"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="9">
        <location>
            <begin position="208"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="10">
        <location>
            <begin position="319"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="7">
        <location>
            <begin position="508"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="572" mass="65382" checksum="A0D2950F3F99B2F8" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHI7</accession>
    <name>B7IHI7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vitamin B12 dependent methionine synthase, activation domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579998.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1095"/>
    <dbReference type="EnsemblBacteria" id="ACJ75551">
        <property type="protein sequence ID" value="ACJ75551"/>
        <property type="gene ID" value="THA_1095"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1095"/>
    <dbReference type="eggNOG" id="COG1410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1446494_0_0_0"/>
    <dbReference type="OMA" id="RISPGYG"/>
    <dbReference type="OrthoDB" id="1410233at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008705">
        <property type="term" value="F:methionine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037010">
        <property type="entry name" value="VitB12-dep_Met_synth_activ_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56507">
        <property type="entry name" value="SSF56507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="181" mass="21203" checksum="5FD9D02648F8197A" modified="2009-02-10" version="1">MIYIPEKFEYMPKKKIVVARASTRYEKLDEELIKKINDLYLEGLSLSSPTIWYETFNIEELPKSIIPEKFKGVKKMTLFVSTLGEEIDKKIEHYTENGEVFSGMILDAWGSEALETLNDNFDKKLRKKYKDGTMRFSPGYEDVDIRENRTIINLLKVDKIKVLESGIMIPRKTTTCMIGWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHL3</accession>
    <name>B7IHL3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA pseudouridine synthase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1124</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1124"/>
    <dbReference type="EnsemblBacteria" id="ACJ75577">
        <property type="protein sequence ID" value="ACJ75577"/>
        <property type="gene ID" value="THA_1124"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1124"/>
    <dbReference type="eggNOG" id="COG1187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024979_1_2_0"/>
    <dbReference type="OMA" id="NAHDKEY"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042092">
        <property type="entry name" value="PsdUridine_s_RsuA/RluB/E/F_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000748">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB/E/F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00093">
        <property type="entry name" value="TIGR00093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="225" mass="25729" checksum="57FB23E4945E41F8" modified="2009-02-10" version="1">MSNAKVGSRKQVKKLIKEKRVKVNGSIVTKPEFNVDENDEVFVDDIEIIPHHNVYIVFNKPSGFTSSKSEYERNIFEFIDHPYLDELHIVGRLDKDVEGLILITNDGNFTHKVISPKSNIEKEYIVKIEGILTKEMIEIASNGITLNNGIKFKPAKITKIDDETISLVITEGKFHEVKLIVGFLGLKYKKIKRVRIGKLNLNDFNLNLGEWKEIDYNTAKKALES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHL6</accession>
    <name>B7IHL6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aspartate aminotransferase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1127</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580017.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1127"/>
    <dbReference type="EnsemblBacteria" id="ACJ75580">
        <property type="protein sequence ID" value="ACJ75580"/>
        <property type="gene ID" value="THA_1127"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1127"/>
    <dbReference type="eggNOG" id="COG0075">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027686_1_1_0"/>
    <dbReference type="OMA" id="IRINHMG"/>
    <dbReference type="OrthoDB" id="996960at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="6">
        <location>
            <begin position="18"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000524-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="338" mass="38476" checksum="BB5B244C231C24E9" modified="2009-02-10" version="1">MEEEILDIGGKPIPYFRTSEFSKMMFEIEEIFKEVIDAPKDSKFALLTASGTGAMEAAVMNIFSEKDKVLVVNGGTFGDRFAKLCKIHQISYDEIKLSFGEKLTKDHLKKFNGRDYTGLLVNIHETSTGQLYDIELLSEFCRQNNMMFVVDAISSFLADEFSMKKYNVDCVILSSQKALALAPGLSFLFLSNRAYTKVLENEVKSMYFDLKDYFQNMERGQTPFTPAVSIVYQLYAKLKSIRENGVESYILKSRNLAQHFRDNVRSLVKIPDYPLSNALTPILVDNAFDIFLRLKEEGIYVTPSGGKLKDKLLRIGHIGNLTKKDNEVLISHLERMVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHP4</accession>
    <name>B7IHP4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prepilin-type N-cleavage/methylation domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580044.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1156"/>
    <dbReference type="EnsemblBacteria" id="ACJ75608">
        <property type="protein sequence ID" value="ACJ75608"/>
        <property type="gene ID" value="THA_1156"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1156"/>
    <dbReference type="eggNOG" id="ENOG5033VZZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_855088_0_0_0"/>
    <dbReference type="OMA" id="YITYVVT"/>
    <dbReference type="OrthoDB" id="1053644at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="338" mass="38779" checksum="CF269B02CC03DB73" modified="2009-02-10" version="1">MKKGFTFSELLVSMLIIAITFQIVYYIVDNSVESYKNSRINLQNLYAESSISLLFDILENELKYAGSGGELLKELYKPVFVPGDQGSYEQKGEVYSQVTDHCKIANSIDYYEDSQKKEFYITYVVTYKNFFVRNGDGTYSPLYGGDVGKINWVIIKNKLSSSADGYYTRIAKLDADKISGPGTYPGEITPSDIFEFKEINLAKTNKTLSLRNEDKYVYPLYEKSVAFTGYPSVMIKQTKIVFEKESGKIYIERYFPIPDSTSNIYISNILENVEDFDINAMYYSNGIKEKPLSEVDSSFDLSSIFALKFVLTWNAPWKYKSKEFKIIKERIIVLIPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHP8</accession>
    <name>B7IHP8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Conserved oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1160</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580048.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1160"/>
    <dbReference type="EnsemblBacteria" id="ACJ75612">
        <property type="protein sequence ID" value="ACJ75612"/>
        <property type="gene ID" value="THA_1160"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1160"/>
    <dbReference type="eggNOG" id="COG3804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050509_1_1_0"/>
    <dbReference type="OMA" id="WGFGAMG"/>
    <dbReference type="OrthoDB" id="803114at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045760">
        <property type="entry name" value="DAP_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19328">
        <property type="entry name" value="DAP_DH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DapB_N" evidence="3">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="DAP_DH_C" evidence="4">
        <location>
            <begin position="134"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="333" mass="36048" checksum="5954CE824C6882B4" modified="2009-02-10" version="1">MRIVSWGLGAMGNGIAKNIIQSGYMKLVGAIDLNHAGKDVGEVLGMDKLGIVVSKDKEIIEKTNPDLVVIATSSFVKEVLPQIEYAVKNHCNVVTIAEEMAFPFDSHPEESLYMDSLARRYGVTILGTGVNPGFVLDTLILSLTGVCNRVDKIVARRINDLSPFGKTVMETQGVGTTPEQFEEGLKTGKIVGHIGFPQSIMMIARALGWNITKIEEERKPIISNVHRETPVVKVEPGMVAGCNHSAKAYIGDEVVIEMQHPQQIHPHLEGVETGDYIEIYGDPDIKLAIKPEIPGGKATIAIATNMIPIVIGAEPGLKTMADLPVPRSVLSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHR4</accession>
    <name>B7IHR4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Heme chaperone HemW</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1178</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probably acts as a heme chaperone, transferring heme to an unknown acceptor. Binds one molecule of heme per monomer, possibly covalently. Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anaerobic coproporphyrinogen-III oxidase family. HemW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1178"/>
    <dbReference type="EnsemblBacteria" id="ACJ75628">
        <property type="protein sequence ID" value="ACJ75628"/>
        <property type="gene ID" value="THA_1178"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1178"/>
    <dbReference type="eggNOG" id="COG0635">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027579_2_2_0"/>
    <dbReference type="OMA" id="HIPWCVR"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034505">
        <property type="entry name" value="Coproporphyrinogen-III_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004559">
        <property type="entry name" value="HemW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13932">
        <property type="entry name" value="PTHR13932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00562">
        <property type="entry name" value="HemN-like__clustered_with_heat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00539">
        <property type="entry name" value="hemN_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="3">
        <location>
            <begin position="6"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU364116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="372" mass="43744" checksum="A794E01885CBC24B" modified="2009-02-10" version="1">MLSDLIGNVNTLSVYIHIPFCKKKCYYCDFVSYEEGSVENYINALSKEIELYGDYLKRGIRTLYIGGGTPSYINEHYIEQLVKKLEKYLILEEFTIEVNPDSFDRQKAQFYKEIGVSRLSIGIQSFDNEVLKKAGRSHDSAQALKAYNVAREYFENVNVDFIIGLPGESWSSIEEIVKFIRENHPPHISVYLLEIHEGTILSNFYKKIPEESYNRYEVLLDFLKSEGYERYEISNFALDKNYSKHNLVYWANLDYIGLGLSSGGHIGNLRYNNVSDFETYYSMLKHDKFPRSYENENTLEREVLESIFMMLRTKWGIDRKLLPILPGFEKVIELLKKKYDFFDGQKLNEMGMDFSSIFFTNLLEIWEDFYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHS7</accession>
    <name>B7IHS7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chemotaxis protein CheW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580071.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1191"/>
    <dbReference type="EnsemblBacteria" id="ACJ75641">
        <property type="protein sequence ID" value="ACJ75641"/>
        <property type="gene ID" value="THA_1191"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1191"/>
    <dbReference type="eggNOG" id="COG0835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048995_3_2_0"/>
    <dbReference type="OMA" id="LNQEMAI"/>
    <dbReference type="OrthoDB" id="1711729at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039315">
        <property type="entry name" value="CheW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617">
        <property type="entry name" value="PTHR22617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="151" mass="17220" checksum="000C3DD011B4C70C" modified="2009-02-10" version="1">MNVETEFEVLSFNICNQEMSFDVDYVEIVIDKDEITPVPKSKEVIEGVINLRGRIIPVVNLRKILGGICSNMDENNFKKIIITKIRDIEMGFLVENVKGVLRTSNSEIDRAFRDVDTYGKKAKGLIKKGERLVVYLDIEEILNEIIGVEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHS9</accession>
    <name>B7IHS9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Flagellar biosynthesis protein FliZ, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1193</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1193"/>
    <dbReference type="EnsemblBacteria" id="ACJ75643">
        <property type="protein sequence ID" value="ACJ75643"/>
        <property type="gene ID" value="THA_1193"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1193"/>
    <dbReference type="eggNOG" id="COG3190">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182369_0_0_0"/>
    <dbReference type="OMA" id="YIVIVRI"/>
    <dbReference type="OrthoDB" id="2024402at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022781">
        <property type="entry name" value="Flagellar_biosynth_FliO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04347">
        <property type="entry name" value="FliO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="88" mass="10170" checksum="68492637A3F5D61C" modified="2009-02-10" version="1">MLGTYLVIKNRVPRNVGGHFISVIERKFITRNSYIVVVRIVEEYYVILVTENGGTVLKKLDSIESGEFENSNFKLEFFKNIVKKGDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHT9</accession>
    <name>B7IHT9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580079.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1206"/>
    <dbReference type="EnsemblBacteria" id="ACJ75653">
        <property type="protein sequence ID" value="ACJ75653"/>
        <property type="gene ID" value="THA_1206"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1206"/>
    <dbReference type="eggNOG" id="ENOG50349X5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_637553_0_0_0"/>
    <dbReference type="OMA" id="CEVRISG"/>
    <dbReference type="OrthoDB" id="1211035at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="407" mass="47103" checksum="FC3B92C5BA3B88EA" modified="2009-02-10" version="1">MKRIVSLIIFYFFSTLFSITIYLNTFDNSLIINQKVYKFSGKALPFKGKIITYFKSPSIEIGNVDIPNDLLVLEDGQTIGQNGTIIVSKDVFDKMVELYTNNNIEKVIIDRFPIEIYDDKIIVKELISRDKLISYLKIFKNNFEITNLDYYLGTYDITPDYPKIEITTFSFPNIGYYNVKIIDKSAVYTKVYVNGKDSKKSGTLTSGTYKIFVKCMDELGYESSKTTFLDIPKSKINFQKIEVEFGKETEFGKINYIGTRNFYEITPLTSTITTITSKDTTSPTINVSIKKLFDDYYSIKVLSNDLNKTKNIILLNNNPINNGVVKLEKGKNLLIVMSKDTFDNYKLKHVSIYNYQTLDSTLTFLDEKRWINIGGVLIKSPYIISWVNPNIERRIYEDKRYIINIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHU0</accession>
    <name>B7IHU0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1207</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580080.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1207"/>
    <dbReference type="EnsemblBacteria" id="ACJ75654">
        <property type="protein sequence ID" value="ACJ75654"/>
        <property type="gene ID" value="THA_1207"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1207"/>
    <dbReference type="eggNOG" id="COG0564">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016902_1_0_0"/>
    <dbReference type="OMA" id="PKTHVYR"/>
    <dbReference type="OrthoDB" id="1714699at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="4">
        <location>
            <begin position="10"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="293" mass="33698" checksum="BBB1BFD511E8ECA0" modified="2009-02-10" version="1">MIVTEENYYSRLDKFLRKKLLNLPLSAIYKLIRTGKIKVNGKKVKNPSYKIEIGDEITIEEDISKYNREINNKVVPIKMDLDIVYEDNDILIINKPAGIPIHPGKGTHIATLIEGLIYYGQEKNFTPHLVHRLDKHTSGILIIAKNTQSARELGDIIAGRSIKKEYIALCKGKLSKEGKIDIPLENKQALTTFITQKVYKTHLGEFSLLNVNIKTGRKHQIRKHLSLINHPIIGDDVYGDKKLNREFKREYGLKRYFLHCHSMEFYFKNKHIAATAPLSKDLKNVLKNLEKGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHX4</accession>
    <name>RL17_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplQ</name>
        <name type="ordered locus">THA_1243</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L32.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101498.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHX4"/>
    <dbReference type="SMR" id="B7IHX4"/>
    <dbReference type="STRING" id="484019.THA_1243"/>
    <dbReference type="EnsemblBacteria" id="ACJ75688">
        <property type="protein sequence ID" value="ACJ75688"/>
        <property type="gene ID" value="THA_1243"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1243"/>
    <dbReference type="eggNOG" id="COG0203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074407_2_2_0"/>
    <dbReference type="OMA" id="YAVTELF"/>
    <dbReference type="OrthoDB" id="1838961at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01368">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_L17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_L17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="PTHR14413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="SSF64263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000144496" description="50S ribosomal protein L17">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01368"/>
        </source>
    </evidence>
    <sequence length="127" mass="14684" checksum="38A12124142A56AE" modified="2009-02-10" version="1">MRHRMKRHRIGRYGSHRKATLRNLSREIVEHGSIITTTPKAKAVQIFFEKLMTKAVKAKKADKKEREVALRREIFKALGDRRLVNKLVDEIAPKYLDRQGGYTAIYKVGPRRGDGAEMSLIKLVIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHX8</accession>
    <name>B7IHX8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101513.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1247"/>
    <dbReference type="EnsemblBacteria" id="ACJ75692">
        <property type="protein sequence ID" value="ACJ75692"/>
        <property type="gene ID" value="THA_1247"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1247"/>
    <dbReference type="eggNOG" id="ENOG502ZQKZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1072613_0_0_0"/>
    <dbReference type="OMA" id="YSVEYGY"/>
    <dbReference type="OrthoDB" id="1575072at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="239" mass="28764" checksum="84FF0121884A26EB" modified="2009-02-10" version="1">MKKVVIFFFLFPLTIFSNILGFWVDPFNALYGFSISQNIEFWNIKALLRYDLPVNWEQGFPLLRPEQLDINFELYSETYSVEYGYFHKKIPFYVSVNPYEKNLNIVWGFTGFYGNYSYFNSDIFDFIFNEDMYGFALKYNDYRFFFENSTENILGFEYNGIVVYFDRFGLEFALNINNENMGFYFVPVNGNFGFVLKDNENFLFLNDDEVSFNWKWGEIYVVGGFQKEKRQFSIEFSIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHZ9</accession>
    <name>B7IHZ9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1268</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580105.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1268"/>
    <dbReference type="EnsemblBacteria" id="ACJ75713">
        <property type="protein sequence ID" value="ACJ75713"/>
        <property type="gene ID" value="THA_1268"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1268"/>
    <dbReference type="eggNOG" id="ENOG5033CSE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_976393_0_0_0"/>
    <dbReference type="OMA" id="MEYAIFY"/>
    <dbReference type="OrthoDB" id="1796881at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="296" mass="35629" checksum="E761A1866F0A7817" modified="2009-02-10" version="1">MRKFFVLEFIIFLIGLFSLIFKEYFLILFLLYMVGNALFTMIYKLDLFQVILSIFMFPYFPLIQFFILKGQTPHYVPLDFEYKPIETINPSYKTIDLAPFHVILKYGDKVQKKEAIKLILELTSKEEIDFNEGLKMIYTTISKENDQDVILYATEASNYLEKTLLRKLYESKDKNSVDYARYSYYYANSPFLEKEQKIELLNNSKKFLEDFIKKYPYNIDALTLLIKILEDLEEHEKIEKLLELKLSKIKSRELFEIAILYYLKYRKHYKVKELLKEFNSYKYTFKNESLKILLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7II01</accession>
    <name>B7II01_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1270"/>
    <dbReference type="EnsemblBacteria" id="ACJ75715">
        <property type="protein sequence ID" value="ACJ75715"/>
        <property type="gene ID" value="THA_1270"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1270"/>
    <dbReference type="HOGENOM" id="CLU_3277929_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="41" mass="5062" checksum="78C38AA9E3B18E1F" modified="2009-02-10" version="1">MESSKIIFQPSSNIILKHFNYFFLIKIVIKELFFLIFFKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMV5</accession>
    <name>C0QMV5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_2029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580551.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2029"/>
    <dbReference type="EnsemblBacteria" id="ACN29522">
        <property type="protein sequence ID" value="ACN29522"/>
        <property type="gene ID" value="THA_2029"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2029"/>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162581_0_0_0"/>
    <dbReference type="OMA" id="NDICSEY"/>
    <dbReference type="OrthoDB" id="2096387at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACN29522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="125" mass="14755" checksum="F91B24AD432C36C7" modified="2009-05-05" version="1">MRKREKYSSDFKLQVVKEYLNSDKSQNDICSEYNISQSAFSRWVKKYEESGYDDNVFNSKKGRPKSIISDISKVPPFIYESAGIVRNDSNKSNDSASLKKRLEYYERLLLEKELLIKMQEDEINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICL1</accession>
    <name>B7ICL1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1293</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101577.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1293"/>
    <dbReference type="EnsemblBacteria" id="ACJ75738">
        <property type="protein sequence ID" value="ACJ75738"/>
        <property type="gene ID" value="THA_1293"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1293"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_1_0"/>
    <dbReference type="OMA" id="TAWNEFP"/>
    <dbReference type="OrthoDB" id="1074175at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="67"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="272" mass="30942" checksum="689B5705749DB3E8" modified="2009-02-10" version="1">MKNTYIVRRIILILITFIYLLPFFWMVGTSLKPDSELLTFPPKIFPENPDWSNYAKALEKFPFFTYLKNSIIITIGNLIGVILSAPFVAYGFSKIKWRGREFLFATMMASIMLPGAVTMIPVFLIYKKLGWLNTFLPLIVPAFLGGGAMNVFLIRQFFNTIPNEILEAARIDGASDFKIFFKIMLPLSKPVLALISLFTFTGSWNNYMGPLIYITSESKYPLALGMPMFMSRYGTYWNQAMAAATLTLIPTIIFFFVAQKYLIEGIKLSGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICM2</accession>
    <name>B7ICM2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA topoisomerase 1</fullName>
            <ecNumber evidence="10">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA topoisomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">topA</name>
        <name evidence="12" type="ordered locus">THA_1304</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580135.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1304"/>
    <dbReference type="EnsemblBacteria" id="ACJ75749">
        <property type="protein sequence ID" value="ACJ75749"/>
        <property type="gene ID" value="THA_1304"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1304"/>
    <dbReference type="eggNOG" id="COG0550">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002929_4_3_0"/>
    <dbReference type="OMA" id="PECKYTR"/>
    <dbReference type="OrthoDB" id="223233at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03363">
        <property type="entry name" value="TOPRIM_TopoIA_TopoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00952">
        <property type="entry name" value="Topoisom_1_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005733">
        <property type="entry name" value="TopoI_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028612">
        <property type="entry name" value="Topoisom_1_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034149">
        <property type="entry name" value="TOPRIM_TopoI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42785">
        <property type="entry name" value="PTHR42785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="zf-C4_Topoisom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="SSF56712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01051">
        <property type="entry name" value="topA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPOISOMERASE_I_PROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="11">
        <location>
            <begin position="5"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="10">
        <location>
            <begin position="161"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="10">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ75749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="700" mass="80714" checksum="871A5E453864274D" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICN8</accession>
    <name>B7ICN8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Stage V sporulation protein S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101650.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1320"/>
    <dbReference type="EnsemblBacteria" id="ACJ75765">
        <property type="protein sequence ID" value="ACJ75765"/>
        <property type="gene ID" value="THA_1320"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1320"/>
    <dbReference type="eggNOG" id="COG2359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168904_1_0_0"/>
    <dbReference type="OMA" id="APNKVAG"/>
    <dbReference type="OrthoDB" id="1985350at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007347">
        <property type="entry name" value="SpoVS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35331">
        <property type="entry name" value="PTHR35331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04232">
        <property type="entry name" value="SpoVS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="90" mass="9597" checksum="0E1C1885F1B2D9B7" modified="2009-02-10" version="1">METLKVSSKSNPNKVAGAIVGSLNKNEKLEIQAIGAGAVNQASKALAVARRFLSEEGKDLYAVPGFIEVEIDGETRTGISFKVYLTKKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICS6</accession>
    <name>B7ICS6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methylglyoxal synthase</fullName>
            <shortName evidence="1">MGS</shortName>
            <ecNumber evidence="1">4.2.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 4" type="primary">mgsA</name>
        <name evidence="4" type="ordered locus">THA_1358</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of methylglyoxal from dihydroxyacetone phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dihydroxyacetone phosphate = methylglyoxal + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17937"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="EC" id="4.2.3.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylglyoxal synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.3" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101725.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1358"/>
    <dbReference type="EnsemblBacteria" id="ACJ75803">
        <property type="protein sequence ID" value="ACJ75803"/>
        <property type="gene ID" value="THA_1358"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1358"/>
    <dbReference type="eggNOG" id="COG1803">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120420_1_0_0"/>
    <dbReference type="OMA" id="RACDVHN"/>
    <dbReference type="OrthoDB" id="1726279at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008929">
        <property type="term" value="F:methylglyoxal synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019242">
        <property type="term" value="P:methylglyoxal biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01422">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00549">
        <property type="entry name" value="Methylglyoxal_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004363">
        <property type="entry name" value="Methylgl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018148">
        <property type="entry name" value="Methylglyoxal_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30492">
        <property type="entry name" value="PTHR30492"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006614">
        <property type="entry name" value="Methylglyox_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00160">
        <property type="entry name" value="MGSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01335">
        <property type="entry name" value="METHYLGLYOXAL_SYNTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MGS-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="55"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1 2">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006614-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="128" mass="14096" checksum="BB32F585173D357E" modified="2009-02-10" version="1">MLNVALIAHDNKKLDLAMFVKEWKNVFEKCNLYATKSTGKIIEQKIGLKVTKLESGPYGGDLQIGSLIVNGEIDFVIFLRDPLTAQPHEPDVSALLRVCDVHNIPLATNLATAEGLVLEIKRKLEGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID15</accession>
    <name>B7ID15_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Methyl-accepting chemotaxis sensory transducer, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1449</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1449"/>
    <dbReference type="EnsemblBacteria" id="ACJ75892">
        <property type="protein sequence ID" value="ACJ75892"/>
        <property type="gene ID" value="THA_1449"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1449"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_639046_0_0_0"/>
    <dbReference type="OMA" id="MIMELSN"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="160"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="364"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="397" mass="44790" checksum="D9D62398A597A681" modified="2009-02-10" version="1">MLIFFVFPQIKNTFKKDILGSFLSEIKIIAKTKSYDDLFDNYQSKGYYFIISKNGTTLNHSDPSKIGKNIADYVPKLFEAMKEKQEGIVEYEFENEKRFAAFAFDGNAYVVHSVTESELFKDYKTLIRNILTIDLPIVIVAILVLGILLSKLLSKDNLKQYDYIKSLFQNISENVLSTSSSTSEIKSMAENTENAINELDKSIDEFAAYMQENSAEIETAINKVKDFTNTIQEIITSSSKLNELTDILTDITEKITDVSDTITVLAINASIETSKETIDREGLSRIAEMIMELSNTTRKLAKDSKNSLMDIEKIITSNILISEKASKETVNVDESLKAIDSVNKASLENIDKLIKISKSTHDAVEELYEGLEQVETAINNISEQVNEFEKAMKNLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID20</accession>
    <name>B7ID20_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chemotaxis protein methyltransferase CheR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580233.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1454"/>
    <dbReference type="EnsemblBacteria" id="ACJ75897">
        <property type="protein sequence ID" value="ACJ75897"/>
        <property type="gene ID" value="THA_1454"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1454"/>
    <dbReference type="eggNOG" id="COG1352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_707410_0_0_0"/>
    <dbReference type="OMA" id="FWSVRFL"/>
    <dbReference type="OrthoDB" id="1378151at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022642">
        <property type="entry name" value="CheR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000780">
        <property type="entry name" value="CheR_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01739">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00996">
        <property type="entry name" value="CHERMTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00138">
        <property type="entry name" value="MeTrc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50123">
        <property type="entry name" value="CHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CheR-type methyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="337" mass="40256" checksum="29DCFDE12E681410" modified="2009-02-10" version="1">MKGKIIQILNKHNLKANPKQIENFYKIIDKEDIDDKTLENLVLMNLTIGESYFFRDKEILEKLKNILKTKKFWKILSVGCSRGEEVYTLSFIAQELDINMEITGIDINKERIKQAREGKYKNWSVRFLNKEQIEKYFEIKDGYFYVKEQYRKNVNFENLNILDLNNTDKKFDIIFLRRVLIYISNPEKIIEKIYSLLKDDGYLVIGLGEYFPDLFKYFSPLLPISSSILVKNPKKIENKQIHKKVLSEIKKQKIELNLEESIVILEKYIEKKEFDSAYKMVKQLSKKFPLAFIVWKYKAYIELELNLKNDAKKSLKRALILNNKDEEIWQLKYAIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID22</accession>
    <name>B7ID22_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1456</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1456"/>
    <dbReference type="EnsemblBacteria" id="ACJ75899">
        <property type="protein sequence ID" value="ACJ75899"/>
        <property type="gene ID" value="THA_1456"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1456"/>
    <dbReference type="eggNOG" id="COG1033">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008861_1_1_0"/>
    <dbReference type="OMA" id="LHFVSHF"/>
    <dbReference type="OrthoDB" id="149255at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="545"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="570"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="596"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="645"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="670"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="7">
        <location>
            <begin position="216"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="703" mass="79567" checksum="DA36EB6303D53C6B" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID92</accession>
    <name>B7ID92_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102058.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1528"/>
    <dbReference type="EnsemblBacteria" id="ACJ75969">
        <property type="protein sequence ID" value="ACJ75969"/>
        <property type="gene ID" value="THA_1528"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1528"/>
    <dbReference type="HOGENOM" id="CLU_117667_0_0_0"/>
    <dbReference type="OMA" id="FTFETSC"/>
    <dbReference type="OrthoDB" id="1809831at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="201" mass="24088" checksum="7D8E25731A6E6718" modified="2009-02-10" version="1">MIKISRNWFFIFIISAFIIVALYFNFNSAKKIYIKGDNYYVGKLIDYLNLNGYKFKIVDVKKANVVIDLDNLEVSLYNGMHFNIKYSEKLINKVISLIENRNCKVLSKNDDFYLKNQYFYKHFTFETSCGINIMILPEMYENVEKLVFRTILNILKGNYQGFENEYFVNKMYIYRLYDNWELIATYEPTLEILELIADEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDA1</accession>
    <name>B7IDA1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1537</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102077.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1537"/>
    <dbReference type="EnsemblBacteria" id="ACJ75978">
        <property type="protein sequence ID" value="ACJ75978"/>
        <property type="gene ID" value="THA_1537"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1537"/>
    <dbReference type="HOGENOM" id="CLU_2541469_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="83" mass="9628" checksum="CDB62630816FAD57" modified="2009-02-10" version="1">MQKLIEYEFKHSVSIEGIADSHLGSFESRFDELVKYLDIDKNSKIILVGDMLDYAIKDSVAMYMNKLKPHRLHKIISRVSTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDB7</accession>
    <name>B7IDB7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Fe-hydrogenase gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1555</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="6">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102231.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1555"/>
    <dbReference type="EnsemblBacteria" id="ACJ75994">
        <property type="protein sequence ID" value="ACJ75994"/>
        <property type="gene ID" value="THA_1555"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1555"/>
    <dbReference type="eggNOG" id="COG1905">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054362_2_1_0"/>
    <dbReference type="OMA" id="LRPVYCL"/>
    <dbReference type="OrthoDB" id="1206880at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="162" mass="18545" checksum="5272B94E5BCCA8C4" modified="2009-02-10" version="1">MLAKEEIKKIVHEAKNERLEEQDILIYILHKIQEKIENNYIPEYAAEVVSEELKIPTSKIYEVLTFYSMFSTKKRGKYVIRVCTSLPCHVANGREIINTLKEELKIDFNQTTSDGLFTLEESGCLGLCGVSPVIMINNEYYGDLTPQKVREIINNLKGGESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDC4</accession>
    <name>B7IDC4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="9">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">gltX2</name>
        <name evidence="9" type="synonym">gltX</name>
        <name evidence="14" type="ordered locus">THA_1563</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102248.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1563"/>
    <dbReference type="EnsemblBacteria" id="ACJ76001">
        <property type="protein sequence ID" value="ACJ76001"/>
        <property type="gene ID" value="THA_1563"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1563"/>
    <dbReference type="eggNOG" id="COG0008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015768_6_3_0"/>
    <dbReference type="OMA" id="HYINTLP"/>
    <dbReference type="OrthoDB" id="1409413at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020752">
        <property type="entry name" value="Glu-tRNA-synth_I_codon-bd_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020061">
        <property type="entry name" value="Glu_tRNA_lig_a-bdl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1c" evidence="12">
        <location>
            <begin position="3"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-synt_1c" evidence="12">
        <location>
            <begin position="125"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_2" evidence="13">
        <location>
            <begin position="315"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="376"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="9">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="9">
        <location>
            <begin position="230"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ76001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="464" mass="54639" checksum="227133913103409F" modified="2009-02-10" version="1">MFRLRFAPSPTGYLHVGGARTALFNWMFARKNNGKFIIRIEDTDTERSKKEFEEKILNALKWLKIDWDEGPDVGGNFGPYRQSERIDIYKKYVDILISEKKAYYAVYHGQEEIEQSYEFPEKYANKENFSIVVKFKVTKQGTTTFNDLLKGKMIFDNNLVDDFIIIKSNGFPTYNFAVVIDDHLMEISHVFRGEDHLSNTQKQIMIYNAFNWQHPVFMHIPLILGNDRTPLSKRHGGTSVDFFKENGFLNKALLNYLAILGWSTSEEIFNVHEKINEFDPFKISNKSVIFDYKKLEWVNGQHLRKIDIEILINEFLNYLKDKNISLEHNSKNYIKEVLLICREKVNTLEQLKEISIPFFFDDYSYEDRYIKKFLLNENAEKILKNALERFEKLQNYTIENVEETLRNISSELEIGTKRVFQTIRGALLGKLVTPGLFESIVVLGKEKTLKRLKKTLELREKYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDD9</accession>
    <name>B7IDD9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Beta-galactosidase</fullName>
            <ecNumber evidence="3 7">3.2.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Lactase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1578</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="3 7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580279.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1578"/>
    <dbReference type="CAZy" id="GH2">
        <property type="family name" value="Glycoside Hydrolase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76016">
        <property type="protein sequence ID" value="ACJ76016"/>
        <property type="gene ID" value="THA_1578"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1578"/>
    <dbReference type="eggNOG" id="COG3250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002346_0_2_0"/>
    <dbReference type="OMA" id="SNWQLQG"/>
    <dbReference type="OrthoDB" id="245411at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009341">
        <property type="term" value="C:beta-galactosidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004199">
        <property type="entry name" value="B-gal_small/dom_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006101">
        <property type="entry name" value="Glyco_hydro_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023232">
        <property type="entry name" value="Glyco_hydro_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006103">
        <property type="entry name" value="Glyco_hydro_2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023230">
        <property type="entry name" value="Glyco_hydro_2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Glyco_hydro_2_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006104">
        <property type="entry name" value="Glyco_hydro_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032312">
        <property type="entry name" value="LacZ_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02929">
        <property type="entry name" value="Bgal_small_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16353">
        <property type="entry name" value="DUF4981"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02836">
        <property type="entry name" value="Glyco_hydro_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02837">
        <property type="entry name" value="Glyco_hydro_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00132">
        <property type="entry name" value="GLHYDRLASE2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01038">
        <property type="entry name" value="Bgal_small_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="SSF49303"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00719">
        <property type="entry name" value="GLYCOSYL_HYDROL_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00608">
        <property type="entry name" value="GLYCOSYL_HYDROL_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bgal_small_N" evidence="8">
        <location>
            <begin position="721"/>
            <end position="988"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="1092" mass="128901" checksum="8A10BAE8387D996C" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDE1</accession>
    <name>B7IDE1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Galactose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="13">2.7.7.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">galT2</name>
        <name evidence="16" type="ordered locus">THA_1580</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12 13">
            <text>alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:13989"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.7.7.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.12" evidence="13"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102323.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1580"/>
    <dbReference type="EnsemblBacteria" id="ACJ76018">
        <property type="protein sequence ID" value="ACJ76018"/>
        <property type="gene ID" value="THA_1580"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1580"/>
    <dbReference type="eggNOG" id="COG1085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029960_1_1_0"/>
    <dbReference type="OMA" id="QIWANEQ"/>
    <dbReference type="OrthoDB" id="1064872at2"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00608">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001937">
        <property type="entry name" value="GalP_UDPtransf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019779">
        <property type="entry name" value="GalP_UDPtransf1_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005850">
        <property type="entry name" value="GalP_Utransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005849">
        <property type="entry name" value="GalP_Utransf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943">
        <property type="entry name" value="PTHR11943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02744">
        <property type="entry name" value="GalP_UDP_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01087">
        <property type="entry name" value="GalP_UDP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000808">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00209">
        <property type="entry name" value="galT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00117">
        <property type="entry name" value="GAL_P_UDP_TRANSF_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 13" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="6 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GalP_UDP_transf" evidence="14">
        <location>
            <begin position="3"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="GalP_UDP_tr_C" evidence="15">
        <location>
            <begin position="179"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-UMP-histidine intermediate" evidence="10 12">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ACJ76018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="318" mass="37826" checksum="59C8C22C5749EF6C" modified="2009-02-10" version="1">MMEIRYNPLTDEWVIISAETQKRPVQPAKNNCPICPGGVEFRDETYDLVAFENRYPSLRKNPPEIKESNKLFKRMKSYGVCEVVVYTSKHDSSFPKMPIFQIEKLVDMWVDRTYELSKNDFVKNIFIFENRGKEVGASLPHPHGQIYAFPFIPKRIEAKLNAMEKWFDENGKCVICEILENETNERLVYENETFVALVPFYARFPFEVHIYPKRHISNLLQFNENEKKDFARILKVVTKKYDKLFEQEFPYMMMFFQAPFNTKDYSDIFHFHVEFNPPKRDKDKLKWMASVETGTWSFINPIVPEQAAKMLREVEVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDF7</accession>
    <name>B7IDF7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1596</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580284.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1596"/>
    <dbReference type="EnsemblBacteria" id="ACJ76034">
        <property type="protein sequence ID" value="ACJ76034"/>
        <property type="gene ID" value="THA_1596"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1596"/>
    <dbReference type="HOGENOM" id="CLU_1414570_0_0_0"/>
    <dbReference type="OMA" id="SYFQFNY"/>
    <dbReference type="OrthoDB" id="2118887at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857021" evidence="1">
        <location>
            <begin position="19"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="193" mass="21862" checksum="8FE3C6373C3BB2FE" modified="2009-02-10" version="1">MKKIIIFMLSLLAISLLATTQGNFIHVSGCTSTSTELSNSIDVRVHQWIDLTWDATPYHICDKETNDQYFDDGFVLLSVEYFSNADVQVSVDFPDPHFRNEFLEILENISIYFFVKSIDAVSNQDSYFQFNYNMGDLTRMGTDTTIPVNLPLVSQGSILFTVNTVTSDPGPYETPAGVYHLPVRFTFNPTVSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDG9</accession>
    <name>B7IDG9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580294.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1608"/>
    <dbReference type="EnsemblBacteria" id="ACJ76046">
        <property type="protein sequence ID" value="ACJ76046"/>
        <property type="gene ID" value="THA_1608"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1608"/>
    <dbReference type="HOGENOM" id="CLU_1165364_0_0_0"/>
    <dbReference type="OMA" id="ENEWINI"/>
    <dbReference type="OrthoDB" id="1316709at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857349" evidence="1">
        <location>
            <begin position="19"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="238" mass="26515" checksum="027B5459E7E14F15" modified="2009-02-10" version="1">MKKLMLILFVITSCILFSDTVVLKDGSQIKGEIQTINSGVLSIKSLVGQIVSYTDIVKEVYFDEQATPTYGVWIEPNEGEWIRIPGKLTKFDGNSGTVLLPDSGTFVFSSREAIKYINFIESSTELDLSQNTSVVPGKTDKLKVTLKNGDTFSAVFSSRLGDIITFIVERGIISIPTNLIEKVIFPENSKYNSLFTLKDGAKIYAKILKFESNSFLIETAIGVIFIDLKMMHNIELLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDK4</accession>
    <name>B7IDK4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Segregation and condensation protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">scpA</name>
        <name evidence="2" type="ordered locus">THA_1643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580321.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1643"/>
    <dbReference type="EnsemblBacteria" id="ACJ76081">
        <property type="protein sequence ID" value="ACJ76081"/>
        <property type="gene ID" value="THA_1643"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1643"/>
    <dbReference type="eggNOG" id="COG1354">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038686_3_3_0"/>
    <dbReference type="OMA" id="YLVMAAM"/>
    <dbReference type="OrthoDB" id="959677at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003768">
        <property type="entry name" value="ScpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33969">
        <property type="entry name" value="PTHR33969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02616">
        <property type="entry name" value="SMC_ScpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="217" mass="25655" checksum="BA8478AA3902A523" modified="2009-02-10" version="1">MELVFKFQEFEGPLDLIIYLVKKKKLSIRDIPLSQLADEFYEYLQQMKNLNLNITSEFIVTASYLMELKSKSLLPRSSEDESFKISKEKFYSQVEQYAALKDLVEKVKNSENLNIEKMPVSVKTVFPKINEKKLKDIIKNAIKEVELKQKVYKIKKEEITIETIMSKVLNSNINKNLYEILKNLNSKYEIIMYFLAILELIKLKKITLDEEFYIRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDM9</accession>
    <name>B7IDM9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="9">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">thrS</name>
        <name evidence="12" type="ordered locus">THA_1668</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-threonyl-tRNA(Thr)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580338.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1668"/>
    <dbReference type="EnsemblBacteria" id="ACJ76106">
        <property type="protein sequence ID" value="ACJ76106"/>
        <property type="gene ID" value="THA_1668"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1668"/>
    <dbReference type="eggNOG" id="COG0441">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008554_0_1_0"/>
    <dbReference type="OMA" id="FYYDFAY"/>
    <dbReference type="OrthoDB" id="900765at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="PTHR11451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 9" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TGS" evidence="11">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="10">
        <location>
            <begin position="243"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Catalytic" evidence="9">
        <location>
            <begin position="243"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="511"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ76106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="636" mass="73934" checksum="C7CE93963D20738E" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDN4</accession>
    <name>B7IDN4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102493.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1673"/>
    <dbReference type="EnsemblBacteria" id="ACJ76111">
        <property type="protein sequence ID" value="ACJ76111"/>
        <property type="gene ID" value="THA_1673"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1673"/>
    <dbReference type="eggNOG" id="COG3314">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_717532_0_0_0"/>
    <dbReference type="OMA" id="WITGNEV"/>
    <dbReference type="OrthoDB" id="819609at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="375" mass="41108" checksum="0E03FFCE875253C1" modified="2009-02-10" version="1">MKKSYFEAFLFLILLILVFLFVNSYMGVSNFFKTLMLTAHDLLLNTVFFIMAVAVITGAFSGLLFEFKVADLIDVILRPFMKPLYNLPGVSVMGIIATYFSDNPAIIALAKDKRFMKNFAKWQEPLLCNLGTSFGMGLIVSTFMIAQGSKMGYNLVPAVFIGNLGAIFGSIVSVRIFSSFTKKKLGDYDNSEEVVNVEYEDGNSYGSFTERLLTALLDGGKKGVDIGLGIIPGVLVISTFVMMITFGPKDPTVGYQGLAYEGIPILPYLGEKIFFILKWLFGFSNPELIAFPLTSLGSTGAALALIPKFIDLNIITPNDIAVFTAMGMTWSGYLSTHIAMMDELGYRFLTGKAILSHTIGGLAAGVFSHFIYMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDP6</accession>
    <name>B7IDP6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH dehydrogenase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580348.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1686"/>
    <dbReference type="EnsemblBacteria" id="ACJ76123">
        <property type="protein sequence ID" value="ACJ76123"/>
        <property type="gene ID" value="THA_1686"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1686"/>
    <dbReference type="eggNOG" id="COG0377">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055737_7_3_0"/>
    <dbReference type="OMA" id="GPYWEHG"/>
    <dbReference type="OrthoDB" id="1904620at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_q6" evidence="1">
        <location>
            <begin position="27"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="180" mass="20993" checksum="E22ABD2AF47CBE8B" modified="2009-02-10" version="1">MKTNERGIWEKIADQLRSKSLWMLYYCTGCGAIELPPSMTSRFDMERFGMGPMATPRQTDILFITGYLSTKTLRRVIYTYEMMSEPKYVVGFGSCTLNGGIYYDSYATINKLDNYIPVDLYIAGCMPRPEALLNAFNELMKMIRNGEANGWKKYKENYEWYKKNQIQALGEVFIHDEFHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDQ6</accession>
    <name>B7IDQ6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cation antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1696</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CPA3 antiporters (TC 2.A.63) subunit E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1696"/>
    <dbReference type="EnsemblBacteria" id="ACJ76133">
        <property type="protein sequence ID" value="ACJ76133"/>
        <property type="gene ID" value="THA_1696"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1696"/>
    <dbReference type="eggNOG" id="COG1863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086615_2_2_0"/>
    <dbReference type="OMA" id="HAMDIED"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002758">
        <property type="entry name" value="Cation_antiport_E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584">
        <property type="entry name" value="PTHR34584"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01899">
        <property type="entry name" value="MNHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019239">
        <property type="entry name" value="MrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="160" mass="18496" checksum="7384D1D3AF501E3C" modified="2009-02-10" version="1">MYISVFIVTYFTWILLTGYNDISELIVGFFVSLIVASVLKKYYRIRFDSKFLVRFVKFVLVYLPVFIWEMIKANFDVAARVLNPKLPINPGFVKVKTNLKKEASKLTLANSITLTPGTLTLDVKDDTLFIHWIDVKTLDENEKKEKISGTFEKILKGVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDR0</accession>
    <name>B7IDR0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76137">
        <property type="protein sequence ID" value="ACJ76137"/>
        <property type="gene ID" value="THA_1700"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1700"/>
    <dbReference type="HOGENOM" id="CLU_1266382_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="218" mass="25326" checksum="60722EB657764E93" modified="2009-02-10" version="1">MRKLVKGCGFFILIVIVMAVAVLLVPSKDKEVAADNVQTPIAQEDIEEDSSLVTEEVESTQESSKIKDFKSYLEALDKEIGYDVLKENLITGKIFNEVNKMQDRNETYSYMIYYLEDKRKILKDIISKLDSLRDRIENSEIYSANQDLYMAAINMDYTVLYLMDAIKETRDHDTVSRSTANFADKYSKERDKYIDKFFGFYKFQVEKQGIEDVFGLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDW2</accession>
    <name>B7IDW2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Flagellar biosynthesis protein FlhA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">flhA</name>
        <name evidence="8" type="ordered locus">THA_1755</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for formation of the rod structure of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FHIPEP (flagella/HR/invasion proteins export pore) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102657.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1755"/>
    <dbReference type="EnsemblBacteria" id="ACJ76189">
        <property type="protein sequence ID" value="ACJ76189"/>
        <property type="gene ID" value="THA_1755"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1755"/>
    <dbReference type="eggNOG" id="COG1298">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015346_3_0_0"/>
    <dbReference type="OMA" id="MAINPGQ"/>
    <dbReference type="OrthoDB" id="436367at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042194">
        <property type="entry name" value="FHIPEP_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042193">
        <property type="entry name" value="FHIPEP_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042196">
        <property type="entry name" value="FHIPEP_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006301">
        <property type="entry name" value="FlhA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001712">
        <property type="entry name" value="T3SS_FHIPEP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30161">
        <property type="entry name" value="PTHR30161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00771">
        <property type="entry name" value="FHIPEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005419">
        <property type="entry name" value="FlhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00949">
        <property type="entry name" value="TYPE3IMAPROT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01398">
        <property type="entry name" value="FlhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="7" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="683" mass="75298" checksum="8BA3AD68FA3445C3" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDW5</accession>
    <name>B7IDW5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76192">
        <property type="protein sequence ID" value="ACJ76192"/>
        <property type="gene ID" value="THA_1761"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1761"/>
    <dbReference type="HOGENOM" id="CLU_3297830_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="40" mass="4732" checksum="CCF24327B635A2DC" modified="2009-02-10" version="1">MLRLLVRDYFWCKIFLAGVALILYSNLTYFYIGQGLLSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDY4</accession>
    <name>B7IDY4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Probable dual-specificity RNA methyltransferase RlmN</fullName>
            <ecNumber evidence="12">2.1.1.192</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">23S rRNA (adenine(2503)-C(2))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">23S rRNA m2A2503 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Ribosomal RNA large subunit methyltransferase N</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">tRNA (adenine(37)-C(2))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">tRNA m2A37 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rlmN</name>
        <name evidence="14" type="ordered locus">THA_1780</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>adenosine(2503) in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(2503) in 23S rRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42916"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10282"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74497"/>
            <dbReference type="EC" id="2.1.1.192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43332"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10485"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74497"/>
            <dbReference type="EC" id="2.1.1.192"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="12">Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the radical SAM superfamily. RlmN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.192" evidence="12"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580405.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1780"/>
    <dbReference type="EnsemblBacteria" id="ACJ76211">
        <property type="protein sequence ID" value="ACJ76211"/>
        <property type="gene ID" value="THA_1780"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1780"/>
    <dbReference type="eggNOG" id="COG0820">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029101_2_0_0"/>
    <dbReference type="OMA" id="QVGCSLD"/>
    <dbReference type="OrthoDB" id="1111428at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070040">
        <property type="term" value="F:rRNA (adenine-C2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002935">
        <property type="term" value="F:tRNA (adenine-C2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01849">
        <property type="entry name" value="RNA_methyltr_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040072">
        <property type="entry name" value="Methyltransferase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027492">
        <property type="entry name" value="RNA_MTrfase_RlmN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004383">
        <property type="entry name" value="rRNA_lsu_MTrfase_RlmN/Cfr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30544">
        <property type="entry name" value="PTHR30544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006004">
        <property type="entry name" value="CHP00048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00275">
        <property type="entry name" value="adenosine_C2_methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00048">
        <property type="entry name" value="rRNA_mod_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="7 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="13">
        <location>
            <begin position="98"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="12">
        <location>
            <begin position="159"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="12">
        <location>
            <begin position="214"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" description="S-methylcysteine intermediate" evidence="12">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ76211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="341" mass="39292" checksum="0E24DA68B2EE5C97" modified="2009-02-10" version="1">MKKNILDFNYEELVNEFKSLGLEKYRVDQVFDWIYKKKVFDFKDMTNLSKEHRRILDENFGIQIPKLLDMQISKIDRTTKFLWELPDGNTIESVALFHEGRVTACISTQVGCPVKCSFCATGQSGYVRNLTAGEIVSQILGIEVHRKIRVGNVVYMGMGEPLLNYENTIKSVKMLNNKKMFGIGIRRITISTVGVPEKIIDLAESGLDVKLALSLHATTNFKRDQIIPLNKQYSIEELIFAVKKYQEITNNRVTIEYILIKEFNDYPEDAEKLAELLKGLSVYVNLIPVNPVNPNYYRPSRWAMERFKEILTKYGIECEIRAEKGTDIDAACGQLRRRNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE02</accession>
    <name>B7IE02_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thiamine thiazole synthase</fullName>
            <ecNumber evidence="4">2.4.2.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">thi4</name>
        <name evidence="5" type="ordered locus">THA_1798</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycine + hydrogen sulfide + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + H(+) + 3 H2O + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:55704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the THI4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.59" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580423.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1798"/>
    <dbReference type="EnsemblBacteria" id="ACJ76229">
        <property type="protein sequence ID" value="ACJ76229"/>
        <property type="gene ID" value="THA_1798"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1798"/>
    <dbReference type="eggNOG" id="COG1635">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053727_2_0_0"/>
    <dbReference type="OMA" id="MWGGGMM"/>
    <dbReference type="OrthoDB" id="1025532at2"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00304">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022828">
        <property type="entry name" value="Thi4_prok"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00292">
        <property type="entry name" value="TIGR00292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="257" mass="28088" checksum="DB79518C579ABBA7" modified="2009-02-10" version="1">MWDYEVSKIIVERFFEKLNDNLNVDVAIVGGGPSALSASYYLSKKGLKVAIFEAKNEPGGGTWGGGMMFNELVVENDIKSFLDELGMNYLIKNNFISVDSVHFASSLLYNATKAGAILFNNVIVEDIVLYENKVNGIVINWAPVIRQKLHVDPITIMAKFVVDGTGHPANVVNMLVDRGIDIDLPIGKIREYPMNAKEGEKFVVENTKEVFPGLYVMGMAAVSVGGGPRMGPIFGGMIKSGLKVANKILEKLSIEVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE30</accession>
    <name>SYD_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aspS</name>
        <name type="ordered locus">THA_1826</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102774.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IE30"/>
    <dbReference type="SMR" id="B7IE30"/>
    <dbReference type="STRING" id="484019.THA_1826"/>
    <dbReference type="PRIDE" id="B7IE30"/>
    <dbReference type="EnsemblBacteria" id="ACJ76257">
        <property type="protein sequence ID" value="ACJ76257"/>
        <property type="gene ID" value="THA_1826"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1826"/>
    <dbReference type="eggNOG" id="COG0173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014330_3_2_0"/>
    <dbReference type="OMA" id="YQLDVEM"/>
    <dbReference type="OrthoDB" id="226836at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00044">
        <property type="entry name" value="Asp_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004524">
        <property type="entry name" value="Asp-tRNA-ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF5">
        <property type="entry name" value="PTHR22594:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="SSF55261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00459">
        <property type="entry name" value="aspS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000199020" description="Aspartate--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="1">
        <location>
            <begin position="194"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="216"/>
            <end position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="476"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="521"/>
            <end position="524"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00044"/>
        </source>
    </evidence>
    <sequence length="581" mass="66506" checksum="CAED65D55A23D946" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE41</accession>
    <name>B7IE41_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Jag protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1837</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102799.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1837"/>
    <dbReference type="EnsemblBacteria" id="ACJ76268">
        <property type="protein sequence ID" value="ACJ76268"/>
        <property type="gene ID" value="THA_1837"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1837"/>
    <dbReference type="eggNOG" id="COG1847">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042512_0_1_0"/>
    <dbReference type="OMA" id="MLDIASW"/>
    <dbReference type="OrthoDB" id="1304273at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02414">
        <property type="entry name" value="jag_KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02644">
        <property type="entry name" value="R3H_jag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038008">
        <property type="entry name" value="Jag_KH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038247">
        <property type="entry name" value="Jag_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039247">
        <property type="entry name" value="KhpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032782">
        <property type="entry name" value="KhpB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034079">
        <property type="entry name" value="R3H_KhpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35800">
        <property type="entry name" value="PTHR35800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14804">
        <property type="entry name" value="Jag_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01245">
        <property type="entry name" value="Jag_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="SSF82708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="R3H" evidence="6">
        <location>
            <begin position="141"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="223" mass="26036" checksum="7432DB00F1E61D31" modified="2009-02-10" version="1">MKKLTITGKSVEEVLAIFEEEMKINKNEYEYLVIDKGSSGFFGFLARDAVVEITIKKEFYERKLEEFLKNIVSYFDSDIKVSVFSKNPRLFIARIDGEGIGKIIGKHGKGLGALQHIATIFLNRLSDIKITVIIDAGDYREKRKELIKKIVENAVEKITSGVERVELDPMFSFERRLVHEFLKKFPHVHSYSEGVEPYRYVVIERRRNHDNIQTRKSRTYNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE42</accession>
    <name>B7IE42_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1838</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
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    </dbReference>
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            <end position="105"/>
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    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="ACJ76269.1"/>
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    </evidence>
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</entry>
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    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
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    </feature>
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            <begin position="424"/>
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</entry>
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        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
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                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
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    </evidence>
    <sequence length="313" mass="34628" checksum="90A7EE0166E829E1" modified="2009-02-10" version="1">MFWTFIGLIIGIILLIKGADWLVEGAVSVALNLGVSKMVVGLSVVAFGTSLPELVASLVAVFKGYSSVSISNVVGSNIANISLALAFSALFVSIPIKKQTIRAEMPFMLISTLTFTVLFLKDYNLVWNYGVIFLLLMIIYIYYLITSAKEIVQEEFEEEVKEVKSTWFSIFLIVLGVVGVSVGGEMTIRNVVKIAEFFNLSETFVGLTIVAVGTSLPEIVVSVISTVKGENDILVGNIVGSNVFNILLILGVSSLFGNLVVDVNNFTIDLIVMNLMAVLLFVFSIFRKRIFRLEAVVFLSMYFYYLYRIILRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
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        </source>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="544"/>
        </location>
    </feature>
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        <location>
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            <end position="581"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="587"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="620"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="6">
        <location>
            <begin position="468"/>
            <end position="636"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="663" mass="76930" checksum="103147FF50053C27" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
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    <name>B7IED3_THEAB</name>
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        <submittedName>
            <fullName evidence="6">Putative transport protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="6" type="ordered locus">THA_1937</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
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        <property type="protein sequence ID" value="ACJ76360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="484019.THA_1937"/>
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        <property type="gene ID" value="THA_1937"/>
    </dbReference>
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        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_1_0"/>
    <dbReference type="OMA" id="QYFLGFP"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="38"/>
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        <location>
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        </location>
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        </location>
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        <location>
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            <end position="172"/>
        </location>
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            <end position="218"/>
        </location>
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    </feature>
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            <begin position="78"/>
            <end position="284"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ACJ76360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="300" mass="34220" checksum="1238A97E8944CCBF" modified="2009-02-10" version="1">MRGSFYYKYIKTPTAKLVLYVLLIGIGYAYLYPLLYMITTSFMSVEDLVNPTIRWIPVHPTLDNYKLAWEVLDVSKTLINSIYLSVVPALIQTFSTAIIAYGLQRFDIPLKKFWLFLVLATFLIPAQTTLVTKYMLFSKLKLVGTPLASFLPALFGQGIKSAIFILLYYNFFQMLPKAFDEAAELDGAGPFRIFWTIMLPLSIPALVTTFIFSLVWYWNETLISGLILGNSIKTLPIALRDFIWRYAQMFPSSTGNSVNRINEGIRLAATFITILPLLITYLFLQRQFVESLERTGITGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEE7</accession>
    <name>B7IEE7_THEAB</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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    </gene>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
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                <person name="Bapteste E."/>
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                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ACJ76374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="THA_1952"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_3223196_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="44" mass="5059" checksum="E263842DD92AD3DC" modified="2009-02-10" version="1">MCKSKKNLELMFVPYLVGIEISFTEPTAFEHNPFVPYLVGIEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEH6</accession>
    <name>B7IEH6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580527.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76403">
        <property type="protein sequence ID" value="ACJ76403"/>
        <property type="gene ID" value="THA_1982"/>
    </dbReference>
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    <dbReference type="eggNOG" id="COG4892">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="1349300at2"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="33"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="1">
        <location>
            <begin position="107"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="1">
        <location>
            <begin position="176"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="289" mass="33434" checksum="DBF041C78A334A6A" modified="2009-02-10" version="1">MKRLLSVFLIVIVFLGFSFTLEDMLNNENMFVILYESLTKNNGKDGSSWVSVNGLVYDLSDVKAWASGMHANGRFKAGKEYTYEIYSISPHGKAIVEKYKPIGILGFTIEDLKKFNSEKFLISYEGIVYDVTNSKTWKEKIGFNLENKEVTYFIKEKKYSDFTTWDNVYQVGILIIPYRDLSMYNGKNGMKAYVAVEGKIYDVSYSKRWKNGNHLNKHSAGEELTYEIIKFSPHGMKKLDNVFKIGYLVFQKNENYTIKENKVFKNGYLIGYCNAPQKIGQKLRNFCKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEI2</accession>
    <name>B7IEI2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1988</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103061.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1988"/>
    <dbReference type="EnsemblBacteria" id="ACJ76409">
        <property type="protein sequence ID" value="ACJ76409"/>
        <property type="gene ID" value="THA_1988"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1988"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_0"/>
    <dbReference type="OMA" id="INNGEIW"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03301">
        <property type="entry name" value="ABC_MalK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015855">
        <property type="entry name" value="ABC_transpr_MalK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040582">
        <property type="entry name" value="OB_MalK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17912">
        <property type="entry name" value="OB_MalK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="365" mass="41410" checksum="A466B13A81D087CB" modified="2009-02-10" version="1">MATLELINLSKRYGKNVWGAKNVNLKVNNGEFIVFLGPSGCGKTTTLRMIAGLEEVTEGKVIIDGKDVTYLEPRKREVSMVFQSYAVWPHMTVYDNIAFPLRLRKMPMDEIDKIVKEVAEMVKIEELLKRFPSQLSGGQRQRVALARALAVKPKIFLMDEPLSNLDAKLRVKMRTELKAIHHRTGATTIFVTHDQSEAMSMADRIVVMRNGKIEQVGTPDDVYFYSANVFVAGFIGTPPTNFFKMNIVRNNGEIHLKNNYIDIKVPEKIKHVIEKYNKDEIIVGIRPENLLITDDEKSAIFREKILVVEPQGSHQVIAVELGDQIVKIVAPAFPKYSADQIIKVTLDHERMMLFDVDTESRLEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEJ7</accession>
    <name>B7IEJ7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_3</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579285.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_3"/>
    <dbReference type="EnsemblBacteria" id="ACJ74511">
        <property type="protein sequence ID" value="ACJ74511"/>
        <property type="gene ID" value="THA_3"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_3"/>
    <dbReference type="HOGENOM" id="CLU_2811039_0_0_0"/>
    <dbReference type="OMA" id="WEIFSEY"/>
    <dbReference type="OrthoDB" id="2068347at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="67" mass="8001" checksum="3D7FD44E378A2E43" modified="2009-02-10" version="1">MLKSSAPKLYESLEKMTFSKFGEKEGEKVFNLLWEIFSEYTNRSESDENKFVDYAKKLIDNYFENRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEL1</accession>
    <name>B7IEL1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_17</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103111.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ74525">
        <property type="protein sequence ID" value="ACJ74525"/>
        <property type="gene ID" value="THA_17"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_17"/>
    <dbReference type="eggNOG" id="ENOG5033KHW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1677022_0_0_0"/>
    <dbReference type="OMA" id="YDVHAGP"/>
    <dbReference type="OrthoDB" id="8169at200918"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857326" evidence="2">
        <location>
            <begin position="22"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="184" mass="20544" checksum="5BD92DDE4CC12B8D" modified="2009-02-10" version="1">MFKKFSVILLSLLVLSASIFAYGPWSNSNQPNNVPQNSSIPLFQNLPKDAKISDITFSGVIKKINLVPGQGSEILIDADGEEYKVHSGPIWLFKDYLETGKTIEVSGKLVTLQDETFVVLEKAVIDGNEFVVRENGFPTYAKRGNNQRGNVSERNNVGRPTQNGRSKRGNQRQGGYENRPCRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEN7</accession>
    <name>B7IEN7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bacterial type II and III secretion system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_43</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial secretin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579313.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_43"/>
    <dbReference type="EnsemblBacteria" id="ACJ74551">
        <property type="protein sequence ID" value="ACJ74551"/>
        <property type="gene ID" value="THA_43"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_43"/>
    <dbReference type="eggNOG" id="COG4796">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_260095_0_0_0"/>
    <dbReference type="OMA" id="FPRSKER"/>
    <dbReference type="OrthoDB" id="870677at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001775">
        <property type="entry name" value="GspD/PilQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004846">
        <property type="entry name" value="T2SS/T3SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00263">
        <property type="entry name" value="Secretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00811">
        <property type="entry name" value="BCTERIALGSPD"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="1318" mass="149579" checksum="340E05B5BA81873E" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IER4</accession>
    <name>B7IER4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="10">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">THA_70</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_70"/>
    <dbReference type="CAZy" id="GT35">
        <property type="family name" value="Glycosyltransferase Family 35"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ74578">
        <property type="protein sequence ID" value="ACJ74578"/>
        <property type="gene ID" value="THA_70"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_70"/>
    <dbReference type="eggNOG" id="COG0058">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010198_1_1_0"/>
    <dbReference type="OMA" id="KHKRTFT"/>
    <dbReference type="OrthoDB" id="137158at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102250">
        <property type="term" value="F:linear malto-oligosaccharide phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102499">
        <property type="term" value="F:SHG alpha-glucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035090">
        <property type="entry name" value="Pyridoxal_P_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="PTHR11468"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000460">
        <property type="entry name" value="Pprylas_GlgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00102">
        <property type="entry name" value="PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000460-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ74578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="831" mass="96708" checksum="EF7B8FCA15054135" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEW1</accession>
    <name>B7IEW1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator, XylR-related</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579358.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_117"/>
    <dbReference type="EnsemblBacteria" id="ACJ74625">
        <property type="protein sequence ID" value="ACJ74625"/>
        <property type="gene ID" value="THA_117"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_117"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_13_5_0"/>
    <dbReference type="OMA" id="INIEHIF"/>
    <dbReference type="OrthoDB" id="1130699at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="371" mass="42695" checksum="6FCC6F5E9EFA48C7" modified="2009-02-10" version="1">MEISTSQKILNELLISYKISRTELEKKLNITPSTLSYTIKKIKDLIEIYKEPSLGQGRPKQYLSLNKDYWISLGVKVGRDYVNITKLNGRFEILERYTFKLSKKNIGNENLSKFLNDAFEKISEKENIKAVGMAFSGEVVEQKINSKILKLENFSPEKIVKTHFKNSVHSILNDVEAIATEEYIKYKGEDILVINYGTGIGACYYGNGELKNNKNRKIIEIGHFFAGGHEKCYCGSTGCLETLASDYAVLKKFKFNNLKIVDFIENEENFENDLKEVRTLYKTFKKRAEDIYEDTFNYLTIFISTIFRILEPKKVILTGEGVTPWFSQMLQKKIINSNKIPIPIIFRGLENNIELGASINALQKYIMTKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEX6</accession>
    <name>B7IEX6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Putative K(+)-stimulated pyrophosphate-energized sodium pump</fullName>
            <ecNumber evidence="9">7.2.3.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Membrane-bound sodium-translocating pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Pyrophosphate-energized inorganic pyrophosphatase</fullName>
            <shortName evidence="9">Na(+)-PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hppA</name>
        <name evidence="10" type="ordered locus">THA_132</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na(+) movement across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>diphosphate + H2O + Na(+)(in) = H(+) + Na(+)(out) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:57884"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Requires K(+) for maximal activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.3.-" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579367.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_132"/>
    <dbReference type="EnsemblBacteria" id="ACJ74640">
        <property type="protein sequence ID" value="ACJ74640"/>
        <property type="gene ID" value="THA_132"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_132"/>
    <dbReference type="eggNOG" id="COG3808">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008743_3_1_0"/>
    <dbReference type="OMA" id="GWKGILF"/>
    <dbReference type="OrthoDB" id="98036at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009678">
        <property type="term" value="F:pyrophosphate hydrolysis-driven proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01129">
        <property type="entry name" value="PPase_energized_pump"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004131">
        <property type="entry name" value="PPase-energised_H-pump"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31998">
        <property type="entry name" value="PTHR31998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03030">
        <property type="entry name" value="H_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001265">
        <property type="entry name" value="H+-PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01104">
        <property type="entry name" value="V_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 9" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="116"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="391"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="483"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="526"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="602"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="691"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="site" description="Determinant of potassium dependence" evidence="9">
        <location>
            <position position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="713" mass="76235" checksum="234C429861A61E2B" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEZ4</accession>
    <name>B7IEZ4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rubrerythrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103596.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_151"/>
    <dbReference type="EnsemblBacteria" id="ACJ74658">
        <property type="protein sequence ID" value="ACJ74658"/>
        <property type="gene ID" value="THA_151"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_151"/>
    <dbReference type="eggNOG" id="COG1633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141525_0_0_0"/>
    <dbReference type="OMA" id="MPEFANP"/>
    <dbReference type="OrthoDB" id="1833195at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubrerythrin" evidence="1">
        <location>
            <begin position="28"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="103" mass="12008" checksum="621F480B618E21DC" modified="2009-02-10" version="1">MPEFSNPFSSLKNDRKLSHEELVRAIRYMIAAEYEAIQLYTQLAESTDNQLAKEVLYDIANEEKVHAGEFLRLLKELSPDEEQFYLEGAKEVEGEIKKLKEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF04</accession>
    <name>B7IF04_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_161</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103623.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_161"/>
    <dbReference type="EnsemblBacteria" id="ACJ74668">
        <property type="protein sequence ID" value="ACJ74668"/>
        <property type="gene ID" value="THA_161"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_161"/>
    <dbReference type="HOGENOM" id="CLU_191490_1_0_0"/>
    <dbReference type="OMA" id="EHEYMKK"/>
    <dbReference type="OrthoDB" id="2081349at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016621">
        <property type="entry name" value="UCP014543"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12646">
        <property type="entry name" value="DUF3783"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="68" mass="8222" checksum="0CA0693866D11D35" modified="2009-02-10" version="1">MKEKAFLIIHGFEKRDELFKLIKLLKDNFPEKELIFATTTPTNLEWKLSDLIVEIEKEHEFMKKTKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF07</accession>
    <name>B7IF07_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_164"/>
    <dbReference type="EnsemblBacteria" id="ACJ74671">
        <property type="protein sequence ID" value="ACJ74671"/>
        <property type="gene ID" value="THA_164"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_164"/>
    <dbReference type="eggNOG" id="COG0426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3158795_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="48" mass="5693" checksum="A4A118580209D8CE" modified="2009-02-10" version="1">MNNICLYDDGKHKYYLLGWEETEDPNMVPTNQYLIVHENNSKGGKKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF18</accession>
    <name>B7IF18_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipase/acylhydrolase, gdsl family, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579392.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_177"/>
    <dbReference type="EnsemblBacteria" id="ACJ74682">
        <property type="protein sequence ID" value="ACJ74682"/>
        <property type="gene ID" value="THA_177"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_177"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083245_0_0_0"/>
    <dbReference type="OMA" id="KSICHEK"/>
    <dbReference type="OrthoDB" id="1247435at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH_hydro" evidence="1">
        <location>
            <begin position="4"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="228" mass="26209" checksum="EC4FD8AD8B9EEC95" modified="2009-02-10" version="1">MYYCFGDSITEGIPGVSYVKYLGKEFLNYGKGGDTTLGLYNRLKNFLNSQTVENIIIEIGTNDILLPFLYNYSPEWQKTVKKIIASGRIPSKNIEEFEKNYKNLLELLVGKNVLIINIPCIGENLNNELNKKVDEYNKIIHKLCKDYSFKLVDFNSWQKSQLKTNSNKYFISKKPFKMVLDSIFVRSSKISNIVSKKRNLVTTIDGVHLNDNSARKLAELIKEKISSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF30</accession>
    <name>HUTU_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Urocanate hydratase</fullName>
            <shortName evidence="1">Urocanase</shortName>
            <ecNumber evidence="1">4.2.1.49</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Imidazolonepropionate hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hutU</name>
        <name type="ordered locus">THA_189</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of urocanate to 4-imidazolone-5-propionate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-imidazolone-5-propanoate = H2O + trans-urocanate</text>
            <dbReference type="Rhea" id="RHEA:13101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17771"/>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
            <dbReference type="EC" id="4.2.1.49"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="1">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the urocanase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.49" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103679.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IF30"/>
    <dbReference type="SMR" id="B7IF30"/>
    <dbReference type="STRING" id="484019.THA_189"/>
    <dbReference type="EnsemblBacteria" id="ACJ74694">
        <property type="protein sequence ID" value="ACJ74694"/>
        <property type="gene ID" value="THA_189"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_189"/>
    <dbReference type="eggNOG" id="COG2987">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018868_0_1_0"/>
    <dbReference type="OMA" id="LVGDWAN"/>
    <dbReference type="OrthoDB" id="100619at2"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00550"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016153">
        <property type="term" value="F:urocanate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00577">
        <property type="entry name" value="HutU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023637">
        <property type="entry name" value="Urocanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035401">
        <property type="entry name" value="Urocanase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038364">
        <property type="entry name" value="Urocanase_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023636">
        <property type="entry name" value="Urocanase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035400">
        <property type="entry name" value="Urocanase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035085">
        <property type="entry name" value="Urocanase_Rossmann-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036190">
        <property type="entry name" value="Urocanase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12216">
        <property type="entry name" value="PTHR12216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01175">
        <property type="entry name" value="Urocanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17392">
        <property type="entry name" value="Urocanase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17391">
        <property type="entry name" value="Urocanase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001423">
        <property type="entry name" value="Urocanate_hydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111326">
        <property type="entry name" value="SSF111326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01228">
        <property type="entry name" value="hutU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01233">
        <property type="entry name" value="UROCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0369">Histidine metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000129581" description="Urocanate hydratase">
        <location>
            <begin position="1"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="46"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="236"/>
            <end position="237"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="257"/>
            <end position="261"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="267"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="486"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00577"/>
        </source>
    </evidence>
    <sequence length="549" mass="61238" checksum="71428368746BBA9A" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF36</accession>
    <name>B7IF36_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAM hydrolase / SAM-dependent halogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_195"/>
    <dbReference type="EnsemblBacteria" id="ACJ74700">
        <property type="protein sequence ID" value="ACJ74700"/>
        <property type="gene ID" value="THA_195"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_195"/>
    <dbReference type="eggNOG" id="COG1912">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059734_0_0_0"/>
    <dbReference type="OMA" id="EGYVGAM"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002747">
        <property type="entry name" value="SAM_Chlor/Fluor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023227">
        <property type="entry name" value="SAM_OH_AdoTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023228">
        <property type="entry name" value="SAM_OH_AdoTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35092">
        <property type="entry name" value="PTHR35092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01887">
        <property type="entry name" value="SAM_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101852">
        <property type="entry name" value="SSF101852"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102522">
        <property type="entry name" value="SSF102522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="189" mass="21727" checksum="BA77F1DD5E8170B6" modified="2009-02-10" version="1">MVDYGVGSSRKAIAAKTENYYFVGPDNGIFTLAFEIEKPIEIRELNNKEFYYGDSQTFHGRDIFSPAAAYIHLGKFEQLGDQLYNYGTLPYIKPKRSGNNIRGEIAYIDKFGNIETNIPYDWIKDFEKVTIVKNRKRIEIPIANYYSEVEKGQLLLHNDSTGYVEIAANQYRANDILKFNTGDLLELIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF46</accession>
    <name>B7IF46_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579412.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_205"/>
    <dbReference type="EnsemblBacteria" id="ACJ74710">
        <property type="protein sequence ID" value="ACJ74710"/>
        <property type="gene ID" value="THA_205"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_205"/>
    <dbReference type="eggNOG" id="ENOG5033DGV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1668574_0_0_0"/>
    <dbReference type="OMA" id="LPNMGKF"/>
    <dbReference type="OrthoDB" id="1473571at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021636">
        <property type="entry name" value="DUF3242"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035951">
        <property type="entry name" value="TM1622-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11586">
        <property type="entry name" value="DUF3242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143477">
        <property type="entry name" value="SSF143477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="158" mass="18376" checksum="18635AC95D0991EA" modified="2009-02-10" version="1">MGKLLVAIVLVLLIAFSCTLIIKTPSLPGSFSLKSAISLLDSYEYPLVETGEINSLYGVQLNRGMYGVFKNFDGMFYVFKYEDEKLTKNDWNRLIKTFGSVLKINYFKFSLFDRGYLQTKFEDLNVITWWKGNWLFVITGNNSKEFFEYINKVYGMIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF58</accession>
    <name>B7IF58_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0111 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103753.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_217"/>
    <dbReference type="EnsemblBacteria" id="ACJ74722">
        <property type="protein sequence ID" value="ACJ74722"/>
        <property type="gene ID" value="THA_217"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_217"/>
    <dbReference type="eggNOG" id="COG1392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104916_0_0_0"/>
    <dbReference type="OMA" id="NSMNPVD"/>
    <dbReference type="OrthoDB" id="1837604at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002727">
        <property type="entry name" value="DUF47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018445">
        <property type="entry name" value="Put_Phosphate_transp_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36536">
        <property type="entry name" value="PTHR36536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01865">
        <property type="entry name" value="PhoU_div"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="223" mass="26058" checksum="EE01A4C6F0D3A7CA" modified="2009-02-10" version="1">MKRFIDRLFPEVSPTKLLSEHAEYCMKAGTIVPQILDDYFNGKDIMQYSKEIDEFESSADEIKTRLREIYTKLRWSYFDRIDALEIIHNQDSIIDAVDDFVKILTMNKVDECPEEVIEKIKLMGDYVADVIKFMKVSVDELRTVVESDFSPNEINKEDNLTFDVEKDESKTDSLGIEIGKTLYSYKNKMNPVDILFLNSIVVLMMKIADRAENVVERIRMIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF79</accession>
    <name>B7IF79_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoesterase</fullName>
            <ecNumber evidence="3">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_240</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579436.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_240"/>
    <dbReference type="EnsemblBacteria" id="ACJ74743">
        <property type="protein sequence ID" value="ACJ74743"/>
        <property type="gene ID" value="THA_240"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_240"/>
    <dbReference type="eggNOG" id="COG0622">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063749_4_0_0"/>
    <dbReference type="OMA" id="IHCGDFV"/>
    <dbReference type="OrthoDB" id="1355703at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00841">
        <property type="entry name" value="MPP_YfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041802">
        <property type="entry name" value="MPP_YfcE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="4">
        <location>
            <begin position="3"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="158" mass="18387" checksum="18A325640E289E66" modified="2009-02-10" version="1">MWLILSDSHDNLHKAKEIERIIIKNNIKKIFHCGDFVAPFILPYFVKEEIEFFGVFGNNDGERLLLTQRSNGKIKVGPRDLTIDNYRILMMHEPFALEAAKSSNLYDFIFFGHTHEIYTEQTEKTLIINPGESSGWLTGKSSIILLEPKNKKWEILYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFB9</accession>
    <name>B7IFB9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uracil permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">uraA</name>
        <name evidence="8" type="ordered locus">THA_283</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579469.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_283"/>
    <dbReference type="EnsemblBacteria" id="ACJ74783">
        <property type="protein sequence ID" value="ACJ74783"/>
        <property type="gene ID" value="THA_283"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_283"/>
    <dbReference type="eggNOG" id="COG2233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017959_1_2_0"/>
    <dbReference type="OMA" id="INHGIGM"/>
    <dbReference type="OrthoDB" id="1115239at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006042">
        <property type="entry name" value="Xan_ur_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00801">
        <property type="entry name" value="ncs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01116">
        <property type="entry name" value="XANTH_URACIL_PERMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="404" mass="42858" checksum="27303251BD701C54" modified="2009-02-10" version="1">MIDLQETVEFYSKVEGPQKFVLALQHFIAMFGATVLVPLLTGFDPLVALFTAGIGTLIFHFLTKKEVPVFLGSSFAFIGPILIVKSQTGDLRYASGGIVAAGFVYLLLATLVKIIGIKKIKKLFPPVVTGPMIMVIGLSLSPVAINWASQNWGISITVIVSVILASTVFKGFFNLIPVLIGVSVGYLVAIITGNVEFSPIINSSWISIPKFMFPKFDLSSILLIAPVALATFMEHIGDITTNGAVVGKNFLEKPGLHRTLIGDGVPTMVAGFLGGPANTTYSENTGVLALTKVYDPSILRLAAVFAIFMSFLSKFGSILQTIPQPVIGGVSLILFGMIASVGIRTIVNEKVDFSKPKNLIVASLILTIGIGGASIKIGTVELKGLALAALVGILANLIIPEKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFG1</accession>
    <name>B7IFG1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_325</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 3 4">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579500.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_325"/>
    <dbReference type="EnsemblBacteria" id="ACJ74825">
        <property type="protein sequence ID" value="ACJ74825"/>
        <property type="gene ID" value="THA_325"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_325"/>
    <dbReference type="eggNOG" id="COG1047">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098197_2_1_0"/>
    <dbReference type="OMA" id="FGQTEDH"/>
    <dbReference type="OrthoDB" id="2044224at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="5">
        <location>
            <begin position="6"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="139" mass="15382" checksum="2C9D5D13A19130BD" modified="2009-02-10" version="1">MGIKSGDKVKVHYVGKFEDGEVFDSSVGKEPLEFVVGMNQVIPGFEEGLMGMEVGEKRTINVPFEKAYGPYREDLVFPVEKSKLPEDVAVDHLLEVHQPDGSSFIVRVSDIKNDMAYLDANHPLAGKNLIFEVEIVSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFJ4</accession>
    <name>B7IFJ4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-acyl-sn-glycerol-3-phosphate acyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_364</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19709"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="2">The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579524.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_364"/>
    <dbReference type="EnsemblBacteria" id="ACJ74858">
        <property type="protein sequence ID" value="ACJ74858"/>
        <property type="gene ID" value="THA_364"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_364"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027938_6_3_0"/>
    <dbReference type="OMA" id="KQRIKLW"/>
    <dbReference type="OrthoDB" id="1756450at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004552">
        <property type="entry name" value="AGP_acyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00530">
        <property type="entry name" value="AGP_acyltrn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="2" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="PlsC" evidence="4">
        <location>
            <begin position="79"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="253" mass="28903" checksum="7A7D7F12B3960054" modified="2009-02-10" version="1">MRKIGSFFYTLYFLIGAVIYIVFYGAIVLFIGFLIGLFNKNLSRRFVVGQIETFGRMAFKLLGIKVYVYGEKPEIGENYLVVANHQSALDIPLIIGYVAPVAFIAKKELGKVPGINWFLKYLGSVLIERGNIRKTAMALKEVVKKLNDGVHFILFPEGTRSPDGSILPFKNRSLEISYKYKVKILPVSIWGAHEVLPKKSLIIRRKPIYIKIHELVDPNQFDSEEELREYVRNVIIEGVKFLEGRNLNEKSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFK6</accession>
    <name>B7IFK6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_376</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_376"/>
    <dbReference type="EnsemblBacteria" id="ACJ74870">
        <property type="protein sequence ID" value="ACJ74870"/>
        <property type="gene ID" value="THA_376"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_376"/>
    <dbReference type="HOGENOM" id="CLU_3259066_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="42" mass="5000" checksum="8DB75414911466D4" modified="2009-02-10" version="1">MSSGLKAESMLIFTDNRVFLDSEYKKNWFEEFKKLVKIKLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFM0</accession>
    <name>B7IFM0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Manganese-dependent transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104119.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_391"/>
    <dbReference type="EnsemblBacteria" id="ACJ74884">
        <property type="protein sequence ID" value="ACJ74884"/>
        <property type="gene ID" value="THA_391"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_391"/>
    <dbReference type="eggNOG" id="COG1321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069532_3_0_0"/>
    <dbReference type="OMA" id="KYFFMYV"/>
    <dbReference type="OrthoDB" id="2047422at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="1">
        <location>
            <begin position="6"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="167" mass="19554" checksum="4E41C5C56BC4F5FD" modified="2009-02-10" version="1">MADKKLTPALENYLVTIYKLSKNDGVTRVSEIAKVKNVSYPSVTTAVKKLAELGYVEHQRYGFVKLTRKGKRVAVTIHNGELRVKYFFMYVIGFPEKWAQQAAELLIYGLDSETRRQLRRFYAIMIDFDETKTEKLIKFLKEQREKLGVKYVPKEALKLKIRLREDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFS5</accession>
    <name>NTPPA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dTTP/UTP pyrophosphatase</fullName>
            <shortName evidence="1">dTTPase/UTPase</shortName>
            <ecNumber evidence="1">3.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside triphosphate pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleotide pyrophosphatase</fullName>
            <shortName evidence="1">Nucleotide PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">THA_448</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dTTP + H2O = diphosphate + dTMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28534"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + UTP = diphosphate + H(+) + UMP</text>
            <dbReference type="Rhea" id="RHEA:29395"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Maf family. YhdE subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.9" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579588.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFS5"/>
    <dbReference type="SMR" id="B7IFS5"/>
    <dbReference type="STRING" id="484019.THA_448"/>
    <dbReference type="EnsemblBacteria" id="ACJ74939">
        <property type="protein sequence ID" value="ACJ74939"/>
        <property type="gene ID" value="THA_448"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_448"/>
    <dbReference type="eggNOG" id="COG0424">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040416_0_0_0"/>
    <dbReference type="OMA" id="RIDGDFY"/>
    <dbReference type="OrthoDB" id="1469203at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036218">
        <property type="term" value="F:dTTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036221">
        <property type="term" value="F:UTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00555">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="PTHR43213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006305">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00172">
        <property type="entry name" value="maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000127797" description="dTTP/UTP pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00528"/>
        </source>
    </evidence>
    <sequence length="191" mass="21615" checksum="D361E6563B98A2C8" modified="2009-02-10" version="1">MKIILASKSPRRIELLKLLKIDFEVIPSNIDENISEKDPKLLAEKLSYLKAMSIKKDGVVLAADTVVTLDKEIFGKPRDYKDAFRMLKSLSGKWHTVITGVTIKFKDEVITFSEKTNVKFKNLSKELIEFYINTAKPFDKAGGYGIQELGSVLVEKIEGDYFNVVGLPISKVWDILWDRGMIDASKGEINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFV1</accession>
    <name>B7IFV1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579603.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_474"/>
    <dbReference type="EnsemblBacteria" id="ACJ74965">
        <property type="protein sequence ID" value="ACJ74965"/>
        <property type="gene ID" value="THA_474"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_474"/>
    <dbReference type="eggNOG" id="ENOG502ZRC8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_811175_0_0_0"/>
    <dbReference type="OMA" id="NQIPEYD"/>
    <dbReference type="OrthoDB" id="971523at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032602">
        <property type="entry name" value="DUF4899"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16240">
        <property type="entry name" value="DUF4899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="329" mass="37660" checksum="9B0DABAC86FE1176" modified="2009-02-10" version="1">MDLYFVKVKGRSKATAEEFVGFLFGKANNAPDFDFLMVPLRFSEQLEEIDLNDNILDFKEKYNKLKNELMKESVVEDLTISFNSFLNTIFNKRGKIAVGIEITAAVKENDMELLKVILSGLIEEWSPKPDLEVVADGLTLEEYSAYNVLDMKEDFEDVIVSELYKRSDLEKLPEFFPVIDPIGGESITKFDVGDVIYVVPVEINKLEEKLRQKYPKNFGEDNKVLPFPARILAKEMVPTKKGYLYLCKVDLGDEIYGKFVVSPSFKLLYDENVLIEKQKNTEKVSEHKVKIVKPANLNVPGGSELFWAFITTMIFSGLLIIILYFLKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFW2</accession>
    <name>B7IFW2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkaline shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asp23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104284.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_485"/>
    <dbReference type="EnsemblBacteria" id="ACJ74976">
        <property type="protein sequence ID" value="ACJ74976"/>
        <property type="gene ID" value="THA_485"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_485"/>
    <dbReference type="eggNOG" id="COG1302">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113198_2_0_0"/>
    <dbReference type="OMA" id="NIQENVH"/>
    <dbReference type="OrthoDB" id="1961470at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005531">
        <property type="entry name" value="Asp23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34297">
        <property type="entry name" value="PTHR34297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03780">
        <property type="entry name" value="Asp23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="109" mass="12326" checksum="532D361DD063E665" modified="2009-02-10" version="1">MKIQTELGELDITLNAIRKLVYFAILETYGPVSISSDSWFSKLLSSEESRVKIQEDEFGHVKVDAYVELEYGTKISEVGRNIIENVKHKLQNLAGCESVEVNVHVIDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFY4</accession>
    <name>B7IFY4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dihydroorotate dehydrogenase electron transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_509</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="5">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="4">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PyrK family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579623.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_509"/>
    <dbReference type="EnsemblBacteria" id="ACJ74998">
        <property type="protein sequence ID" value="ACJ74998"/>
        <property type="gene ID" value="THA_509"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_509"/>
    <dbReference type="eggNOG" id="COG0543">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_1_2_0"/>
    <dbReference type="OMA" id="LNEYMAC"/>
    <dbReference type="OrthoDB" id="1885467at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037117">
        <property type="entry name" value="Dihydroorotate_DH_ele_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DHODB_Fe-S_bind" evidence="6">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="66"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="215" mass="24193" checksum="D0743B6F175AF64A" modified="2009-02-10" version="1">MENTYLTKKDVIQINEDTFIVTFNEKFKFDEGQFIMIQTPSLTRKPFILGTWEDNIAVSVQIKGKGTNYIVFQGEKFKAHFPLGNKFIPPAGKGIVIASPTCITLANLLHEKYSCNVLIGSKSKINLELPFESVVGNEDFSKKIKTLKTYDWYLVSGSKQMEEFVLSNIESKNVFVSLEEYMGCGIGACKSCAVFTKDGVKHVCTDGPIFRRDML</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFZ7</accession>
    <name>B7IFZ7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycoside hydrolase, family 57</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_522</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycosyl hydrolase 57 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579629.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_522"/>
    <dbReference type="CAZy" id="GH57">
        <property type="family name" value="Glycoside Hydrolase Family 57"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75011">
        <property type="protein sequence ID" value="ACJ75011"/>
        <property type="gene ID" value="THA_522"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_522"/>
    <dbReference type="eggNOG" id="COG1543">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008192_1_0_0"/>
    <dbReference type="OMA" id="CAYYEGL"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030979">
        <property type="term" value="P:alpha-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037090">
        <property type="entry name" value="57_glycoside_trans_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015293">
        <property type="entry name" value="BE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040042">
        <property type="entry name" value="Branching_enz_MT3115-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004300">
        <property type="entry name" value="Glyco_hydro_57_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41695">
        <property type="entry name" value="PTHR41695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09210">
        <property type="entry name" value="DUF1957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03065">
        <property type="entry name" value="Glyco_hydro_57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="SSF88688"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_57" evidence="3">
        <location>
            <begin position="7"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1957" evidence="4">
        <location>
            <begin position="429"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="537" mass="63378" checksum="D93C7013454B3C78" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG09</accession>
    <name>B7IG09_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_535</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100635.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_535"/>
    <dbReference type="EnsemblBacteria" id="ACJ75023">
        <property type="protein sequence ID" value="ACJ75023"/>
        <property type="gene ID" value="THA_535"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_535"/>
    <dbReference type="eggNOG" id="ENOG50343NN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1805246_0_0_0"/>
    <dbReference type="OMA" id="FENQYAG"/>
    <dbReference type="OrthoDB" id="2024239at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007809">
        <property type="entry name" value="FlgN-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05130">
        <property type="entry name" value="FlgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="144" mass="16918" checksum="E573E3F15127D513" modified="2009-02-10" version="1">MKDLLKNQINLLKSIIESFKLAERALIEKDVQNLTRHISKVEEYSFDFERLEEELNKEINKMGYKSIKEYIEKTNDSEVAYLLATLVENLNELTIVMSNFKNLVDFENKYFEFIKSLFSNSSTTSTYTKKGYGVNQKSLIDKVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG11</accession>
    <name>B7IG11_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">THA_537</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579639.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_537"/>
    <dbReference type="EnsemblBacteria" id="ACJ75025">
        <property type="protein sequence ID" value="ACJ75025"/>
        <property type="gene ID" value="THA_537"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_537"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_19_0"/>
    <dbReference type="OMA" id="QTAKQMQ"/>
    <dbReference type="OrthoDB" id="477199at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033479">
        <property type="entry name" value="dCache_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029151">
        <property type="entry name" value="Sensor-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02743">
        <property type="entry name" value="dCache_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103190">
        <property type="entry name" value="SSF103190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="11">
        <location>
            <begin position="306"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="10">
        <location>
            <begin position="377"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="371"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ75025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="663" mass="72605" checksum="63F342F70B9CEEA2" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG16</accession>
    <name>B7IG16_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100609.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_542"/>
    <dbReference type="EnsemblBacteria" id="ACJ75030">
        <property type="protein sequence ID" value="ACJ75030"/>
        <property type="gene ID" value="THA_542"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_542"/>
    <dbReference type="eggNOG" id="COG4112">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115729_0_0_0"/>
    <dbReference type="OMA" id="FKQPIPY"/>
    <dbReference type="OrthoDB" id="1310481at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="54"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="195" mass="22656" checksum="5433FF2C7805CB9F" modified="2009-02-10" version="1">MYENEKVLVVPTEDVERLCNKRHGLIFVNEYDIISLIENKGFFVDRNSAETDETIRQVIPYIVLREENKFLLFKRTSKQGEKRLHNQITIGVGGHINTDDSLEPLEAFKNGLIREINEEVDVDIKSLNYVGVINVVDTPVSRVHVGICYVADVKYKGLKEKDKFIELFTENPREYFEEMEGWSKTVVQSLELMQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG22</accession>
    <name>B7IG22_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tetratricopeptide repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_548</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579643.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_548"/>
    <dbReference type="EnsemblBacteria" id="ACJ75036">
        <property type="protein sequence ID" value="ACJ75036"/>
        <property type="gene ID" value="THA_548"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_548"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_497693_0_0_0"/>
    <dbReference type="OMA" id="YDYRYHF"/>
    <dbReference type="OrthoDB" id="1428822at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="69"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="103"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="170"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="491" mass="58517" checksum="633701408DFEFB47" modified="2009-02-10" version="1">MPLITEMDRIPLDVIVRGLEAQYSVEKTDYWATYLVYFYYEKFKKLLNEQKYEEARKILEKAKNVLYDYRYHFYYGLLFAKLGDYDNAEIELKQAVSLNPNFYIGYYELGNVLYLKKDYDEAIEIYMKAFELNKEFSLPLLKIGDTYFENGQFKDAEIAYKSALKIEKLPQVYLRLGVLYNELQKFEKAEKIFRDGLSVEYKPEIAYNLSYTLIRLGKHFQALQILKELANNYPTPEVYNELGLLQKNLGLYEDAEENLKLAGEEYEENYLKILLYTKGYDHKILDKLTKYDPEYVDFLKKVLFKLKDKLEEKLSNINFQFDELEKVFDLTDEKGEIILDEFSKIEAKDFNQKLSYIISSAYIAGADPILIEKNLVKTSMVLFKIDKIVSFYKVFQIIYFGRLFEKLNFEDLIDKSLEVLSEYDFPLMKKAQHMLEIERTSLEDFWENYTEPNDLEEFILKFLELLSYEPTQYELDENTFLGKLMKFILNI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG44</accession>
    <name>ATPB_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit beta</fullName>
            <ecNumber evidence="1">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F1 sector subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpD</name>
        <name type="ordered locus">THA_571</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100559.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IG44"/>
    <dbReference type="SMR" id="B7IG44"/>
    <dbReference type="STRING" id="484019.THA_571"/>
    <dbReference type="EnsemblBacteria" id="ACJ75058">
        <property type="protein sequence ID" value="ACJ75058"/>
        <property type="gene ID" value="THA_571"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_571"/>
    <dbReference type="eggNOG" id="COG0055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022398_0_2_0"/>
    <dbReference type="OMA" id="GFNMIMD"/>
    <dbReference type="OrthoDB" id="430176at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_1000143556" description="ATP synthase subunit beta">
        <location>
            <begin position="1"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="156"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01347"/>
        </source>
    </evidence>
    <sequence length="470" mass="51843" checksum="B0E56DF954DE02F6" modified="2009-02-10" version="1">MSKKSKGKILRVIGPVVDVQFEEGDLPDIYDALVVINPQTGKKLVLEVEQLIGDNAVRTVALDTTDGLMRGLEVENTGEPIKVPVGKGALGRMFNVIGEPIDGKEDEIKDVEYWPIHKAPPSITEQSTSVEILETGIKVIDLLAPFPKGGKIGFFGGAGVGKTVLVMELIRNIAIEHHGFSMFAGVGERTREGNELYLDMQEAEVLDNTVLVFGQMNEPPGARFRVALTALTMAEYFRDVEGRDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLATDMGELQERITSTKKGSITSVQAIYVPADDITDPAPATTFTHLDATVVLSRRRAALGLYPAVDPLDSTSKMLDPNIIGQEHYEVARGVQEILQRYKDLQDIIAILGMEELSEEDKLIVQRARKIERFLSQPVHVAEKFSNIPGKYVPINETIRGFKEILEGKYDDLPEMAFYMVGTIDEAVEKAKQLQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG54</accession>
    <name>B7IG54_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase I</fullName>
            <ecNumber evidence="6">2.1.1.198</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA 2'-O-ribose C1402 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">rRNA (cytidine-2'-O-)-methyltransferase RsmI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rsmI</name>
        <name evidence="8" type="ordered locus">THA_581</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(1402) in 16S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42924"/>
            <dbReference type="Rhea" id="RHEA-COMP:10285"/>
            <dbReference type="Rhea" id="RHEA-COMP:10286"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.198"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the methyltransferase superfamily. RsmI family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.198" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100540.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_581"/>
    <dbReference type="EnsemblBacteria" id="ACJ75068">
        <property type="protein sequence ID" value="ACJ75068"/>
        <property type="gene ID" value="THA_581"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_581"/>
    <dbReference type="eggNOG" id="COG0313">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044779_4_0_0"/>
    <dbReference type="OMA" id="RTMVFFE"/>
    <dbReference type="OrthoDB" id="1059309at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070677">
        <property type="term" value="F:rRNA (cytosine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000453">
        <property type="term" value="P:enzyme-directed rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11648">
        <property type="entry name" value="RsmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01877">
        <property type="entry name" value="16SrRNA_methyltr_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008189">
        <property type="entry name" value="rRNA_ssu_MeTfrase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018063">
        <property type="entry name" value="SAM_MeTrfase_RsmI_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46111">
        <property type="entry name" value="PTHR46111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005917">
        <property type="entry name" value="MTase_YraL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00096">
        <property type="entry name" value="TIGR00096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01296">
        <property type="entry name" value="RSMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="232" mass="26403" checksum="10775E9141CFD3A0" modified="2009-02-10" version="1">MLYLIGTPIGNLEDITLRALRILREEIDYIFAEDTRRALKLLNFFDIKKPVDSFNEHSSIKKKNKILELLKDNKNVAYISDAGMPVISDPGTELVNICLENNIPWDVIPGPSAPITALSASGFYGNRFLFLGFMPRDKKRRKLLRMIKDENLADVFVFFESPERLLKTLKDILEIIGDVEIFIARELTKIHQEYFKGAISEALEHFSGGVKGEITVVFKNKLLTKNNNGCIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG77</accession>
    <name>B7IG77_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_605</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579677.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_605"/>
    <dbReference type="EnsemblBacteria" id="ACJ75091">
        <property type="protein sequence ID" value="ACJ75091"/>
        <property type="gene ID" value="THA_605"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_605"/>
    <dbReference type="eggNOG" id="COG1672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041137_3_0_0"/>
    <dbReference type="OMA" id="PLKEEPY"/>
    <dbReference type="OrthoDB" id="801313at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004256">
        <property type="entry name" value="DUF234_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03008">
        <property type="entry name" value="DUF234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_2" evidence="1">
        <location>
            <begin position="7"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="DUF234" evidence="2">
        <location>
            <begin position="315"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="460" mass="54641" checksum="5BFF56F268DD5456" modified="2009-02-10" version="1">MGGDIIFINRFEEKKLLNEILTSSKKEVFILYGRRRVGKSALLKEVSKNKRTLFYTARKISKTEQLNSFSRSVGIFLNMGNIRFENWEDAFRTLFNYSKRENIVIILDEFQYLAEKNEEIISILQILLDEFDDSKIKLILCGSSISFMEGILNYKNPLYGRKTGSLKLNPISFENLKLFIPEYNYHQLIETYSIIGGIPYYLTLWNGKTDIFSNIENLFLKLGAPLKEEPYFILYQELREPAIYHSILEALASGRNKINEITSFIGENDSRKIQPYLKSLINLKLIKRITPALLKNPHRTKNFLYVIDYQLFRFWYRYIFPYKESVDLNEYENVLNLIENDFSQYVSFEFEKQSVNYLKRRFNLIEAGNFWKKDVEIDMFGKDKEGKIYAAEIKWRNKKMNIKDFYNLKNKVEKIALNVDYFILVSKSGFEENLSEIDDNVYFIEFSKEKGWKDWHGVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG86</accession>
    <name>B7IG86_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_614</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104412.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_614"/>
    <dbReference type="EnsemblBacteria" id="ACJ75100">
        <property type="protein sequence ID" value="ACJ75100"/>
        <property type="gene ID" value="THA_614"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_614"/>
    <dbReference type="HOGENOM" id="CLU_187610_0_0_0"/>
    <dbReference type="OMA" id="AMMRDPC"/>
    <dbReference type="OrthoDB" id="2080601at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="67" mass="7746" checksum="4F2CFB7296A57ACB" modified="2009-02-10" version="1">MYEKVLIYTAKEPQGKAKEMLKDLKNVEVFITLNFYKDAKVKAKKLGNCELKDLGDFGERAMMRDPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG92</accession>
    <name>B7IG92_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_620"/>
    <dbReference type="EnsemblBacteria" id="ACJ75106">
        <property type="protein sequence ID" value="ACJ75106"/>
        <property type="gene ID" value="THA_620"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_620"/>
    <dbReference type="eggNOG" id="COG3234">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1336984_0_0_0"/>
    <dbReference type="OMA" id="QGPTPRW"/>
    <dbReference type="OrthoDB" id="1378174at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009558">
        <property type="entry name" value="DUF1175"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06672">
        <property type="entry name" value="DUF1175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="205" mass="24050" checksum="2676F16EAEEFAF7E" modified="2009-02-10" version="1">MAICTTLLFPIGMNEGFSKLALDLNDSLNFRLWFVSIALDNVDNEHPSFKTKDCAGFVFYSLKEALKKHDMNWIKKTGYRGPIFEDVKKYNYPDTPLSVNIFFDGKDFVPYVTAYNLLMYNVEFVSFERKHAKVGDLVFFFHPEDPDFPFHVMIYTTNGFVYHTGPGGEIRYVRFEDMMLGDMTWRPIRLNKAFLGYFRLKFLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGC6</accession>
    <name>B7IGC6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycerol uptake operon antiterminator regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_663</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104523.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_663"/>
    <dbReference type="EnsemblBacteria" id="ACJ75140">
        <property type="protein sequence ID" value="ACJ75140"/>
        <property type="gene ID" value="THA_663"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_663"/>
    <dbReference type="eggNOG" id="COG1954">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111516_0_1_0"/>
    <dbReference type="OMA" id="PAIRNMK"/>
    <dbReference type="OrthoDB" id="1560657at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006699">
        <property type="entry name" value="GlpP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35787">
        <property type="entry name" value="PTHR35787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04309">
        <property type="entry name" value="G3P_antiterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016897">
        <property type="entry name" value="GlpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="183" mass="20573" checksum="BF50E2C5C16D3A29" modified="2009-02-10" version="1">MHPFIYPIVPAIRNLKMVEKIISTQVSSVFLLEGDIFEIKRACKKLKEHGKFVFVHVDLVEGIGKDKASVKLVKEFVGADGIITTRSNLIDFARKLDLMAIQRIFLIDSKAFQTGIEQVKKHKANFVEVLPGLIPELVRKIKEEIVQPIISGGLVTTKEQAKLLLENGAIGVSTSYEKLWDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGI7</accession>
    <name>B7IGI7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579761.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_734"/>
    <dbReference type="EnsemblBacteria" id="ACJ75201">
        <property type="protein sequence ID" value="ACJ75201"/>
        <property type="gene ID" value="THA_734"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_734"/>
    <dbReference type="eggNOG" id="COG1637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_859492_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="348" mass="40340" checksum="43222CE182315FAA" modified="2009-02-10" version="1">MEFTIRIDELNNKLKSKFFIVILSNEENLKKCVENNIAGFPETDNGVWAYLDINEGDYVSFYYNGRIFNLYKVEKKFIPEHYKKSNNSIGNEMYDPVPLNNGDKWRAISTKKGNLYFPYRLSLKLLEKTDFLTSLVFKSGFERFGINLIPRVSLKKSHFQLSLSDINKIFGIENLSVSLSSFTLKDFAEVFSKNKRINKEKFSVENIVDKEIFLQTLIKRILEASIYKYKKVVFDKISFGNDSFEFFSEQTVYGGEADIVIAYNSENIAFVEVKNGIILKKNGEFTRIGSEAYDQVKSYKDIIDPNKKIRKIIAGKKDFGSKDVLLKIFEKKSENIMVFEVNSSFEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGL4</accession>
    <name>B7IGL4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FecR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100305.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_763"/>
    <dbReference type="EnsemblBacteria" id="ACJ75228">
        <property type="protein sequence ID" value="ACJ75228"/>
        <property type="gene ID" value="THA_763"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_763"/>
    <dbReference type="eggNOG" id="COG4254">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_933624_0_0_0"/>
    <dbReference type="OMA" id="WISISKG"/>
    <dbReference type="OrthoDB" id="1473030at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FecR" evidence="1">
        <location>
            <begin position="152"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="298" mass="33857" checksum="18D117B949A165F0" modified="2009-02-10" version="1">MKKIIFLISLIPLILFSNSFTLFTGDSTTIKILSKGNFKVSVIDVLGGVLPEDILIDELSFKDIATITYIAPIVPMDTYLKLKVDGKIIDYNFIVVEPSFPQDATFVTLEEFSGNVAISKDGQNWSPVSKKEKLYEGIILKTLDNSYAVISGNWGKLYVKPNSTVKILRSRSNNKDFDFIVEVEKGEVVADVLKFIMSKSRFQIKKDSVTAGVRGTRFGVKDNTWFVFEGTVYLFNGNRLISLGAKKLVKVIENTFENPQDFEEEFETYIDTFDKLFEDLEKEIDKQMEEWDIELPGI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGM6</accession>
    <name>NDK_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="1">NDK</shortName>
            <shortName evidence="1">NDP kinase</shortName>
            <ecNumber evidence="1">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ndk</name>
        <name type="ordered locus">THA_778</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100342.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IGM6"/>
    <dbReference type="SMR" id="B7IGM6"/>
    <dbReference type="STRING" id="484019.THA_778"/>
    <dbReference type="EnsemblBacteria" id="ACJ75240">
        <property type="protein sequence ID" value="ACJ75240"/>
        <property type="gene ID" value="THA_778"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_778"/>
    <dbReference type="eggNOG" id="COG0105">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060216_6_3_0"/>
    <dbReference type="OMA" id="KIVAMKM"/>
    <dbReference type="OrthoDB" id="1752581at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006165">
        <property type="term" value="P:nucleoside diphosphate phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="SSF54919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDP_KINASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000125026" description="Nucleoside diphosphate kinase">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <sequence length="147" mass="16987" checksum="642D90606295508D" modified="2009-02-10" version="1">MERTFVYLKPNAVRRGLVGEIIKRFEQRGIKIVALKLLWMSKQQAEKLYEMHKGKSFYNDLIDFVTGGPIVAMIVEAPRVIEMVRHIIGDTDPLKAGTGTIRGEFALTITKNLIHASDSKENFEREYKIFFSDDEIIDYYLDVQDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGP8</accession>
    <name>B7IGP8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA polymerase III PolC-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100377.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_800"/>
    <dbReference type="EnsemblBacteria" id="ACJ75262">
        <property type="protein sequence ID" value="ACJ75262"/>
        <property type="gene ID" value="THA_800"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_800"/>
    <dbReference type="eggNOG" id="COG0847">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047806_7_1_0"/>
    <dbReference type="OMA" id="FHVYLNP"/>
    <dbReference type="OrthoDB" id="1985371at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006054">
        <property type="entry name" value="DnaQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00573">
        <property type="entry name" value="dnaq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="1">
        <location>
            <begin position="6"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="186" mass="21441" checksum="3295724D21E45A31" modified="2009-02-10" version="1">MLDENVYCVMDTETTGLNPYFGDRIIEIAIVPVYKNKIVKKWIYHSLVNPNIRIPALTEKIHGISNDAITDAPSLENIISNVRAYAKGTIFVMHNAYVDLSFIDIATKEIGEFPINFRYIDTLEISQVLYNKKRSLESLVKEFKIADKVPHRALEDAILTAKVFLKLYEKIGYNNINEFIRIWGDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGQ4</accession>
    <name>PLSX_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate acyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.274</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acyl-ACP phosphotransacylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Acyl-[acyl-carrier-protein]--phosphate acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphate-acyl-ACP acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">plsX</name>
        <name type="ordered locus">THA_806</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a fatty acyl-[ACP] + phosphate = an acyl phosphate + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:42292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.274"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Probably interacts with PlsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Associated with the membrane possibly through PlsY.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PlsX family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.274" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579808.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IGQ4"/>
    <dbReference type="SMR" id="B7IGQ4"/>
    <dbReference type="STRING" id="484019.THA_806"/>
    <dbReference type="EnsemblBacteria" id="ACJ75268">
        <property type="protein sequence ID" value="ACJ75268"/>
        <property type="gene ID" value="THA_806"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_806"/>
    <dbReference type="eggNOG" id="COG0416">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039379_1_1_0"/>
    <dbReference type="OMA" id="HGKSNAR"/>
    <dbReference type="OrthoDB" id="631784at2"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043811">
        <property type="term" value="F:phosphate:acyl-[acyl carrier protein] acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00019">
        <property type="entry name" value="PlsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003664">
        <property type="entry name" value="FA_synthesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012281">
        <property type="entry name" value="Phospholipid_synth_PlsX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30100">
        <property type="entry name" value="PTHR30100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02504">
        <property type="entry name" value="FA_synthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002465">
        <property type="entry name" value="Phsphlp_syn_PlsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00182">
        <property type="entry name" value="plsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000116384" description="Phosphate acyltransferase">
        <location>
            <begin position="1"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00019"/>
        </source>
    </evidence>
    <sequence length="327" mass="35358" checksum="BD321D0394244971" modified="2009-02-10" version="1">MKKIAIDLMGGDYAPEEILAGALSFAKENEDVELYLVGIEENFKDVQLPKNCVKVVTDDFLPMDVKPTEAVRRRKSTMYVSCQLAREKKVDAVVSAGNTGALLACATFVVGRIKGIERPTLAVPIPTKNDFCVLADAGANIDVKPSNLLQFAIMGVEYAKLLGKDNPTIGLLNVGTEENKGTQKEKEAFQILKERFGNQFVGNVEGNDLNAGKVDVVVADGFHGNIAMKTMEGAAKMITELIKSEVKKNIISALGALLMKPVFSSLKNKLDPKKYGGTFFIGVEGVVVKAHGNSNRTAIFNALKVAKKGVEEKLPLKIKEALLKCAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGS0</accession>
    <name>B7IGS0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_822</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579816.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_822"/>
    <dbReference type="EnsemblBacteria" id="ACJ75284">
        <property type="protein sequence ID" value="ACJ75284"/>
        <property type="gene ID" value="THA_822"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_822"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_0"/>
    <dbReference type="OMA" id="CANEMEK"/>
    <dbReference type="OrthoDB" id="881434at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="21"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="338"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="371"/>
            <end position="378"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="536" mass="62471" checksum="E04C97DE3052C548" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGZ7</accession>
    <name>B7IGZ7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methicillin resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_902</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579871.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_902"/>
    <dbReference type="EnsemblBacteria" id="ACJ75361">
        <property type="protein sequence ID" value="ACJ75361"/>
        <property type="gene ID" value="THA_902"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_902"/>
    <dbReference type="eggNOG" id="COG0472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_990215_0_0_0"/>
    <dbReference type="OMA" id="MCLGFLP"/>
    <dbReference type="OrthoDB" id="728505at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="281" mass="32962" checksum="C2C9EFBCCE809523" modified="2009-02-10" version="1">MKLLAYFLITLVIYYISKKYNILLDYPTERKNHHKPTPQVGGVILFTILLFFFDYPFLVLFSLLLFGILDDMFKLSYKQKFVFEIVIAIYIVYRYKLTLFEIHNIFTDIFATLWIVAFFNGLNMIDGLNGLSTGVSIIYLSMLYQFELALSYLPIYIFNFFGKLFMGESGILITGFILLSSAVSLENDMVYLTIFFGYPFYEVVASFIRRVISKENPFLADRKHLHHIFPQKMGNLFLPFSYLLAFLFASLPKSYMSIFYYLSISIILFLIHFSLIKKLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH02</accession>
    <name>B7IH02_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_907</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100777.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_907"/>
    <dbReference type="EnsemblBacteria" id="ACJ75366">
        <property type="protein sequence ID" value="ACJ75366"/>
        <property type="gene ID" value="THA_907"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_907"/>
    <dbReference type="eggNOG" id="COG3951">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182367_0_0_0"/>
    <dbReference type="OMA" id="ESFGEKW"/>
    <dbReference type="OrthoDB" id="2021892at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="98" mass="11459" checksum="D0EA3A5EC78ACF90" modified="2009-02-10" version="1">MDVSKVSQNTVNQINVDKLKKVSEEFVASFFFQIFRKMYDTIPKSSLIPESFGEKWFRENMLYEYSKIAAKNDLKDLSMSVYRSLGGKVYNQIPNTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH29</accession>
    <name>B7IH29_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_934</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579889.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_934"/>
    <dbReference type="EnsemblBacteria" id="ACJ75393">
        <property type="protein sequence ID" value="ACJ75393"/>
        <property type="gene ID" value="THA_934"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_934"/>
    <dbReference type="eggNOG" id="ENOG503360Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_524537_0_0_0"/>
    <dbReference type="OMA" id="IFVHIDS"/>
    <dbReference type="OrthoDB" id="279466at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="SSF50969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="499" mass="57446" checksum="B259487A18BB5E9E" modified="2009-02-10" version="1">MKRLVPLLYLLVSVVSFSILTEEQSRAFLSEAIKLWSEGKYAEASLKMDDAMTGSISAREIPWFWYVKAKIDVYNDQVDAARENLKTLLTITNIPEIMELYKKIEVFNSPENFEVKEYKLKFVNSVSGKEGLTEYFYSPVGLAVFGEKVYVIDKKNKRLIVLDDFKMSNIIKLPYNPRSIAVDSFGKVYISSEDSIYTLENQQPIYTGLKSPIIAGVDRANRLVVVDASKIYIFSDRVIEKNLPLPTIALDCDINYENLYILDAFSNQILVYNLYTLELKDKIKLKDKIWSFEVTPAGQLIYFDGNKIIAGESEFKINSTPMFIEYSYPHLFVVDWKSDTINWYLLKDDLPIFVKVESFETSDATLTAHVCLENYFGDDIYLAKDFLNIYEEDVKEFAQIEGEQDTVYPTTDISKSLLTDRFLGKVIVKNDRWKFTGGAPIDFEKRGKFKWKITYNYAKIFPLPLVKVSVKFEVADEKFTDIFVYTEEVVNGGEANKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH35</accession>
    <name>B7IH35_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_940</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100848.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_940"/>
    <dbReference type="EnsemblBacteria" id="ACJ75399">
        <property type="protein sequence ID" value="ACJ75399"/>
        <property type="gene ID" value="THA_940"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_940"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_0_0"/>
    <dbReference type="OMA" id="LTMTNGQ"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="71"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="306" mass="34555" checksum="A71531B7D0388A7A" modified="2009-02-10" version="1">MRKKNLFIGLLVITPSIIAIAIFVYGFIGWTVRTSFSNWNSFSALLRGQYKFVGLRNYIRLFQDSRFQTDLWNTFFFTLFFLLGTIALGIIMAILVDKGLKGSRFFQNLFLFPMAISFVVTGTVWSWIFAPGNIPQNPQGLNLLLSKLGLEKLMWGWYTSTHSIGKFNVALIPVIIAAIWQLSGYTMAMYLAGLRGISEDIIEAARVDGASEWKIFWKIKMPLLTPITLSAMIILGHISLKIFDLVFSMTGSGPNFVTDVPAIYMFELTFRSNRYAMGSAIAIIMLCFVAVVIIPYLATNLRGERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH36</accession>
    <name>B7IH36_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter, carbohydrate uptake transporter-1 (CUT1) family, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_941</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579891.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_941"/>
    <dbReference type="EnsemblBacteria" id="ACJ75400">
        <property type="protein sequence ID" value="ACJ75400"/>
        <property type="gene ID" value="THA_941"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_941"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_2_0"/>
    <dbReference type="OMA" id="YIVFQRQ"/>
    <dbReference type="OrthoDB" id="1584669at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="64"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="270" mass="30158" checksum="E11E4B222641E4A5" modified="2009-02-10" version="1">MKKFFSYFFLIIIIAIYIITPLYVLLATSFKPLSEIGISDMWKLPKSWSLSGLIKAFNKLSPNLKNSFYLTIPATIVSALLGSLNGYVFSKVKFKGVNIIFAIILFGMFIPYQSVLFPLIRFLQKVNLYGTITGLIIVHVVYGIPITSLIFRNYYSEVPNELIEAAYMDGAGFFKTYSKVLFPISIPAFVVVIIWQFTNIWNEFLFAVTVTSNPAKQPITVALVNLAGSQVVEWNVQMAGALITALPTLVVYILLGKYFIRGLLSGSVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH38</accession>
    <name>B7IH38_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_943</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75402">
        <property type="protein sequence ID" value="ACJ75402"/>
        <property type="gene ID" value="THA_943"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_943"/>
    <dbReference type="HOGENOM" id="CLU_3067155_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="53" mass="6445" checksum="FD9659F1C81EB516" modified="2009-02-10" version="1">MKSLKKQFFFISLFLILSFTILFGVLLTYTLYKVETKQLKELIYQKTKQFLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHA7</accession>
    <name>B7IHA7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DDE_Tnp_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75471">
        <property type="protein sequence ID" value="ACJ75471"/>
        <property type="gene ID" value="THA_1013"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1013"/>
    <dbReference type="HOGENOM" id="CLU_2144673_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="1">
        <location>
            <begin position="4"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="130" mass="15932" checksum="568A46AD6B5EA63C" modified="2009-02-10" version="1">MLLDFEIGNNREVEHAEIMLEDIEGSILLADRGYWQWEFIEKMNEKMKLYIRSRGRKGKEFMEREINRLIYSKRWEIEWYIERLKQRIKMRGMNERTLKAQITYMLLGQQLTMLIDTLSAQPKMIRLIEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHD7</accession>
    <name>COBS_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenosylcobinamide-GDP ribazoletransferase</fullName>
            <ecNumber evidence="1">2.7.8.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cobalamin synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cobalamin-5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cobS</name>
        <name type="ordered locus">THA_1043</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosylcob(III)inamide-GDP + alpha-ribazole = adenosylcob(III)alamin + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16049"/>
            <dbReference type="ChEBI" id="CHEBI:10329"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosylcob(III)inamide-GDP + alpha-ribazole 5'-phosphate = adenosylcob(III)alamin 5'-phosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="ChEBI" id="CHEBI:60493"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CobS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.26" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579952.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHD7"/>
    <dbReference type="STRING" id="484019.THA_1043"/>
    <dbReference type="EnsemblBacteria" id="ACJ75501">
        <property type="protein sequence ID" value="ACJ75501"/>
        <property type="gene ID" value="THA_1043"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1043"/>
    <dbReference type="eggNOG" id="COG0368">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057426_1_2_0"/>
    <dbReference type="OMA" id="FMDSMDG"/>
    <dbReference type="OrthoDB" id="1996371at2"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00238"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051073">
        <property type="term" value="F:adenosylcobinamide-GDP ribazoletransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008818">
        <property type="term" value="F:cobalamin 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00719">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003805">
        <property type="entry name" value="CobS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148">
        <property type="entry name" value="PTHR34148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02654">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_1000189622" description="Adenosylcobinamide-GDP ribazoletransferase">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00719"/>
        </source>
    </evidence>
    <sequence length="232" mass="26308" checksum="58898C4C5FE556DB" modified="2009-02-10" version="1">MIKNFLLSLSFISRLPLNINVNDFDRRVKKLPSFFPLVGYIVGSIYYLGALSNNLALKVFFLVLAFYFFDLFHFDGFLDTLDGFLNQSTKEKRLEIMSKGDVGPFAVFFGTLFVVVFWNLYLNANPFYFFISSTFGRYSMVLLMAFSKPAKKEGLGALFFPFEKKNLLISTIFTFFLIIFFKQYIISLTVTLITTYLISKIATSKIGGVTGDVLGGTCLFVNGLILLILEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHE7</accession>
    <name>B7IHE7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1055"/>
    <dbReference type="EnsemblBacteria" id="ACJ75511">
        <property type="protein sequence ID" value="ACJ75511"/>
        <property type="gene ID" value="THA_1055"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1055"/>
    <dbReference type="HOGENOM" id="CLU_3259069_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="42" mass="5022" checksum="7D15B6462080F1E0" modified="2009-02-10" version="1">MKMYVLNKVNNNINLLLKLMIIKFFGKILLEKFIILKREGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHG2</accession>
    <name>B7IHG2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Decarboxylating precorrin-6Y C5,15-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579976.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1070"/>
    <dbReference type="EnsemblBacteria" id="ACJ75526">
        <property type="protein sequence ID" value="ACJ75526"/>
        <property type="gene ID" value="THA_1070"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1070"/>
    <dbReference type="eggNOG" id="COG2242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094143_0_0_0"/>
    <dbReference type="OMA" id="RCKFVYA"/>
    <dbReference type="OrthoDB" id="1706318at2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014008">
        <property type="entry name" value="Cbl_synth_MTase_CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02469">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="181" mass="20512" checksum="08ED97631AF84357" modified="2009-02-10" version="1">MYFEDEFFLHENTPITKSEIRAIVYSKLNLNKKDVFWDIGAGSGAVSIEASFLCKKVFAVEKKLERVKTINENIRRAKAKNIDVILGEAPQALEDLPSPTKVFIGGSEDKTFEILTAVERKILNGGIIVATSITLETLYTIDDFYKGQKKEIIQVGVTKLSSHKKRMFIARNPIFIITVWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHH5</accession>
    <name>B7IHH5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cobalt transport protein CbiN</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Energy-coupling factor transporter probable substrate-capture protein CbiN</fullName>
            <shortName evidence="10">ECF transporter S component CbiN</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cbiN</name>
        <name evidence="11" type="ordered locus">THA_1083</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the energy-coupling factor (ECF) transporter complex CbiMNOQ involved in cobalt import.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms an energy-coupling factor (ECF) transporter complex composed of an ATP-binding protein (A component, CbiO), a transmembrane protein (T component, CbiQ) and 2 possible substrate-capture proteins (S components, CbiM and CbiN) of unknown stoichimetry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CbiN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579989.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1083"/>
    <dbReference type="EnsemblBacteria" id="ACJ75539">
        <property type="protein sequence ID" value="ACJ75539"/>
        <property type="gene ID" value="THA_1083"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1083"/>
    <dbReference type="eggNOG" id="COG1930">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136197_2_0_0"/>
    <dbReference type="OMA" id="YKAKGRY"/>
    <dbReference type="OrthoDB" id="2073796at2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00330">
        <property type="entry name" value="CbiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003705">
        <property type="entry name" value="CbiN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38662">
        <property type="entry name" value="PTHR38662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02553">
        <property type="entry name" value="CbiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01165">
        <property type="entry name" value="cbiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 10" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0170">Cobalt</keyword>
    <keyword evidence="1 10" id="KW-0171">Cobalt transport</keyword>
    <keyword evidence="7 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="99" mass="10929" checksum="4380CF1EBD1F4A7F" modified="2009-02-10" version="1">MEKKHIYMLVASLVIVIFSFIINSGAEFAGADGIAEEVISQINQDYSPWFSPLWEPPSGEIESLLFSLQAAIGALIIGYYFGFLKGKRQVKNDSGKNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHI5</accession>
    <name>B7IHI5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Iodotyrosine dehalogenase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579996.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1093"/>
    <dbReference type="EnsemblBacteria" id="ACJ75549">
        <property type="protein sequence ID" value="ACJ75549"/>
        <property type="gene ID" value="THA_1093"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1093"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_1_0_0"/>
    <dbReference type="OMA" id="WVDQDLQ"/>
    <dbReference type="OrthoDB" id="1847197at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="181" mass="21216" checksum="6A03D54D5A0210F6" modified="2009-02-10" version="1">MIFDLAKKRKTTRIFLNEKVNIEDIKYAINVAKEAPSGMNSQPWHFVIVDDEKLKEKIRKSVEEKEKIFYEKSKGKLKDFLNEYNITWQKSFLTEAPYLILVYSDKRFPFSKESTWISVGYLLLALEEKGLSSLTYTPPNPQDFNFSTYKLEVIIPVGHGNDPKPKLKRKDLNEIISYNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHJ8</accession>
    <name>B7IHJ8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cyclic diguanylate phosphodiesterase (EAL) domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1109</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
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                <person name="Bapteste E."/>
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                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
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            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
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                <person name="Bowman S."/>
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            <dbReference type="PubMed" id="19124572"/>
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</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
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                <person name="Bowman S."/>
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            </authorList>
            <dbReference type="PubMed" id="19124572"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
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        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FliJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1145"/>
    <dbReference type="EnsemblBacteria" id="ACJ75597">
        <property type="protein sequence ID" value="ACJ75597"/>
        <property type="gene ID" value="THA_1145"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1145"/>
    <dbReference type="eggNOG" id="COG2882">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141683_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012823">
        <property type="entry name" value="Flagell_FliJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02050">
        <property type="entry name" value="FliJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="136" mass="16556" checksum="05DFABAE7028D1E5" modified="2009-02-10" version="1">MLDFASKQEELIKQELFKVRMEKKKIEEEIQKTVDYLKSLENEIFGKQINGLYLNMLLEIKNSGEKYIESLKDKLYEISLMEEKVLKDYLEKRKERKSLEKLKERFKNKEYIELQRKDIKNIDEIAERKHFFGGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHQ2</accession>
    <name>B7IHQ2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methylmalonyl-CoA mutase, C-domain/subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1164</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101320.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1164"/>
    <dbReference type="EnsemblBacteria" id="ACJ75616">
        <property type="protein sequence ID" value="ACJ75616"/>
        <property type="gene ID" value="THA_1164"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1164"/>
    <dbReference type="eggNOG" id="COG5012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027795_0_0_0"/>
    <dbReference type="OMA" id="AWKVMPE"/>
    <dbReference type="OrthoDB" id="44644at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028991">
        <property type="entry name" value="KamE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036843">
        <property type="entry name" value="KamE_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015130">
        <property type="entry name" value="Lys-AminoMut_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037086">
        <property type="entry name" value="Lys-AminoMut_asu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09043">
        <property type="entry name" value="Lys-AminoMut_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16554">
        <property type="entry name" value="OAM_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="SSF51703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="4" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="5">
        <location>
            <begin position="593"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="730" mass="81906" checksum="0B81E21BC49AD74C" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHT7</accession>
    <name>B7IHT7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Pyruvate kinase</fullName>
            <ecNumber evidence="4 12">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">THA_1204</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="4 12"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580077.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1204"/>
    <dbReference type="EnsemblBacteria" id="ACJ75651">
        <property type="protein sequence ID" value="ACJ75651"/>
        <property type="gene ID" value="THA_1204"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1204"/>
    <dbReference type="eggNOG" id="COG0469">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015439_0_2_0"/>
    <dbReference type="OMA" id="QVPIVQK"/>
    <dbReference type="OrthoDB" id="1098983at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PTHR11817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="SSF52935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 12 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 15" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PK" evidence="13">
        <location>
            <begin position="1"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="PK_C" evidence="14">
        <location>
            <begin position="354"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ACJ75651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="470" mass="52154" checksum="803260912E00F9CA" modified="2009-02-10" version="1">MRKTRIVATIGPATESEEMLEKLVNSGVNVFRLNSSHDTIEIHRERIRRLKKIREKLNTPFSILIDLSGPKIRTGKLKEEYITLKEGSILKITTEDLLGDESILSVNYKRFPLEVKSGDRILLNDGAIELTVEKVEDNIVYTKVIRGGKITHHRGVNLPGVDLSISAVTDKDKKFIDLAIEEDLDYIALSFVRKASDVKYAKSLSNGIPVIAKIETAQALDNLEEIISFADGVMVARGDLGVEIPLSQVPIAQKRIIETANRMSKPVITATQMLESMINNMTPTRAEVSDIANAILDGTDAIMLSAETSIGKYPLRAVAVMDEVAKNTEKFLLDYDSIELEWIRNYYISENIEDAISHAVYNLSRDINAKLIITATSTGKTAINIARLRPSVPIMAATPNLSTYYRLSLVWGVIPVMINQTLSTDEMIIEVMRKAKETNLASKGDKVIITAGIPWGRPGTTNTLQVHEII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHU2</accession>
    <name>RS7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsG</name>
        <name type="ordered locus">THA_1211</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S9 and S11.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101430.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHU2"/>
    <dbReference type="SMR" id="B7IHU2"/>
    <dbReference type="STRING" id="484019.THA_1211"/>
    <dbReference type="EnsemblBacteria" id="ACJ75656">
        <property type="protein sequence ID" value="ACJ75656"/>
        <property type="gene ID" value="THA_1211"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1211"/>
    <dbReference type="eggNOG" id="COG0049">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072226_1_1_0"/>
    <dbReference type="OMA" id="NVMPHVE"/>
    <dbReference type="OrthoDB" id="1540940at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_B">
        <property type="entry name" value="Ribosomal_S7_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_S5/S7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005717">
        <property type="entry name" value="Ribosomal_S7_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_S7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_S7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_S7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="PTHR11205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="SSF47973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01029">
        <property type="entry name" value="rpsG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_1000126014" description="30S ribosomal protein S7">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <sequence length="155" mass="17944" checksum="73303B07767C53FA" modified="2009-02-10" version="1">MRRRRAEVRKVPPDPIYNDVLVSKLINRVMWDGKKSIAQKIVYKAMEYLSEKTKKDPLEALHQAIDNVRPLVEVRPRRVGGATYQVPIEVEEPRKTSLALRWIVEAARSKKGRPMAEKLGEELVNAFNNTGTAIKKKEDVHRMAEANRAFAHFRW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHV7</accession>
    <name>RL24_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplX</name>
        <name type="ordered locus">THA_1226</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101460.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHV7"/>
    <dbReference type="SMR" id="B7IHV7"/>
    <dbReference type="STRING" id="484019.THA_1226"/>
    <dbReference type="EnsemblBacteria" id="ACJ75671">
        <property type="protein sequence ID" value="ACJ75671"/>
        <property type="gene ID" value="THA_1226"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1226"/>
    <dbReference type="eggNOG" id="COG0198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093315_2_0_0"/>
    <dbReference type="OMA" id="HVKPTQE"/>
    <dbReference type="OrthoDB" id="2040741at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_B">
        <property type="entry name" value="Ribosomal_L24_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="KOW_RPL26/RPL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003256">
        <property type="entry name" value="Ribosomal_L24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_L24/26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12903">
        <property type="entry name" value="PTHR12903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17136">
        <property type="entry name" value="ribosomal_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01079">
        <property type="entry name" value="rplX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000142049" description="50S ribosomal protein L24">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <sequence length="106" mass="11774" checksum="3B5D9A93C6D9188A" modified="2009-02-10" version="1">MARKIRKGDTVMVLSGKDKGKTGEVVRVIPKEDKVVVRGVNVVKRHQRPNAQMRQGGIIEKESPIYACKVALVCPSCGKATRVGFRFLEDGTKVRYCKKCGEVIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHY0</accession>
    <name>B7IHY0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipoprotein signal peptidase</fullName>
            <ecNumber evidence="6">3.4.23.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Prolipoprotein signal peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Signal peptidase II</fullName>
            <shortName evidence="6">SPase II</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">lspA</name>
        <name evidence="9" type="ordered locus">THA_1249</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">This protein specifically catalyzes the removal of signal peptides from prolipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.</text>
            <dbReference type="EC" id="3.4.23.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; lipoprotein biosynthesis (signal peptide cleavage).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8">Belongs to the peptidase A8 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.36" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580088.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1249"/>
    <dbReference type="EnsemblBacteria" id="ACJ75694">
        <property type="protein sequence ID" value="ACJ75694"/>
        <property type="gene ID" value="THA_1249"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1249"/>
    <dbReference type="eggNOG" id="COG0597">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083252_3_1_0"/>
    <dbReference type="OrthoDB" id="1575081at2"/>
    <dbReference type="UniPathway" id="UPA00665"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00161">
        <property type="entry name" value="LspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001872">
        <property type="entry name" value="Peptidase_A8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33695">
        <property type="entry name" value="PTHR33695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01252">
        <property type="entry name" value="Peptidase_A8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00781">
        <property type="entry name" value="LIPOSIGPTASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00077">
        <property type="entry name" value="lspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00855">
        <property type="entry name" value="SPASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="182" mass="21139" checksum="2B0F46704886171A" modified="2009-02-10" version="1">MFWITLAFVIDQITKYIATEYWRFNPTRLFNFFYLTYATNKGVAFGLFSNSKEIVIYLTLAIIIGLSIIPLFKRLNFWTNMFLGFIIGGALGNVVDRIRFGYVVDFITMPYWPTIYNLADFFILVGGIGIGLVLLWRRDIGDQRNSERERLEIGQIRSGESTELDFKDVHSESDKDWGDKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHY7</accession>
    <name>B7IHY7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1256</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75701">
        <property type="protein sequence ID" value="ACJ75701"/>
        <property type="gene ID" value="THA_1256"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1256"/>
    <dbReference type="HOGENOM" id="CLU_3349761_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="37" mass="4379" checksum="B56AA523BDEBB46C" modified="2009-02-10" version="1">MNQKNVSIPNRYAKNWENGKKRKKVEVFQSLIGMLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMT7</accession>
    <name>C0QMT7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase repeat family ISCc3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_2011</name>
        <name evidence="2" type="ordered locus">THA_2021</name>
        <name evidence="3" type="ordered locus">THA_2023</name>
        <name evidence="4" type="ordered locus">THA_2025</name>
        <name evidence="5" type="ordered locus">THA_2028</name>
        <name evidence="6" type="ordered locus">THA_2030</name>
        <name evidence="7" type="ordered locus">THA_2032</name>
        <name evidence="8" type="ordered locus">THA_2034</name>
        <name evidence="9" type="ordered locus">THA_2035</name>
        <name evidence="10" type="ordered locus">THA_2036</name>
        <name evidence="11" type="ordered locus">THA_2043</name>
    </gene>
    <organism evidence="1 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 12">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2011"/>
    <dbReference type="EnsemblBacteria" id="ACN29504">
        <property type="protein sequence ID" value="ACN29504"/>
        <property type="gene ID" value="THA_2011"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29514">
        <property type="protein sequence ID" value="ACN29514"/>
        <property type="gene ID" value="THA_2021"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29516">
        <property type="protein sequence ID" value="ACN29516"/>
        <property type="gene ID" value="THA_2023"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29518">
        <property type="protein sequence ID" value="ACN29518"/>
        <property type="gene ID" value="THA_2025"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29521">
        <property type="protein sequence ID" value="ACN29521"/>
        <property type="gene ID" value="THA_2028"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29523">
        <property type="protein sequence ID" value="ACN29523"/>
        <property type="gene ID" value="THA_2030"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29525">
        <property type="protein sequence ID" value="ACN29525"/>
        <property type="gene ID" value="THA_2032"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29527">
        <property type="protein sequence ID" value="ACN29527"/>
        <property type="gene ID" value="THA_2034"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29528">
        <property type="protein sequence ID" value="ACN29528"/>
        <property type="gene ID" value="THA_2035"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29529">
        <property type="protein sequence ID" value="ACN29529"/>
        <property type="gene ID" value="THA_2036"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACN29536">
        <property type="protein sequence ID" value="ACN29536"/>
        <property type="gene ID" value="THA_2043"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2011"/>
    <dbReference type="KEGG" id="taf:THA_2021"/>
    <dbReference type="KEGG" id="taf:THA_2023"/>
    <dbReference type="KEGG" id="taf:THA_2025"/>
    <dbReference type="KEGG" id="taf:THA_2028"/>
    <dbReference type="KEGG" id="taf:THA_2030"/>
    <dbReference type="KEGG" id="taf:THA_2032"/>
    <dbReference type="KEGG" id="taf:THA_2034"/>
    <dbReference type="KEGG" id="taf:THA_2035"/>
    <dbReference type="KEGG" id="taf:THA_2036"/>
    <dbReference type="KEGG" id="taf:THA_2043"/>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_36_0_0"/>
    <dbReference type="OMA" id="MVAECEQ"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACN29504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACN29514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACN29516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACN29518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACN29521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACN29523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACN29525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACN29527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACN29528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACN29529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACN29536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="96" mass="10877" checksum="9A1A62D61A549D7D" modified="2009-05-05" version="1">MRRKFSAEEKMAIVLEGLRGKKTIAEICREHGISQGQYYKWRDKFLEGAKTALETSGNSSQAAQYQRKIQELEQIIGKQAIVIETLKKTVIHPGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMU1</accession>
    <name>C0QMU1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transposase repeat family IS605</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_2015</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2015"/>
    <dbReference type="EnsemblBacteria" id="ACN29508">
        <property type="protein sequence ID" value="ACN29508"/>
        <property type="gene ID" value="THA_2015"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2015"/>
    <dbReference type="eggNOG" id="COG0675">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_9_1_0"/>
    <dbReference type="OMA" id="INIECEG"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_IS605" evidence="5">
        <location>
            <begin position="27"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="6">
        <location>
            <begin position="155"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACN29508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="259" mass="29485" checksum="BEEB1FA2007092EA" modified="2009-05-05" version="1">MHRKFPNEFKIKNVTFERTATNKYYVSIVIEVPDTKITNTSDVVIGIDLGIKDTLTLSTGEKYSLPDLSKYEKKLKRLHRQLSRKQKGSKNREKARLCLAKVYEKITNIKNDWIHKVTHKIVNENQVGKIVVEDLNIKGMMKNSKLSKYIHMQSWGKFITILTYKAKNLGIEVIKANRFFPSSQTCSVCGYVNKEVKDLKIRKWVCPICGTEHDRDINAAKNLAKYGLMLPVGREPSEFTPVDSALAAEPAKGLRAITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICM4</accession>
    <name>B7ICM4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alanine racemase</fullName>
            <ecNumber evidence="3">5.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1306</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-alanine = D-alanine</text>
            <dbReference type="Rhea" id="RHEA:20249"/>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="5.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alanine racemase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580137.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1306"/>
    <dbReference type="EnsemblBacteria" id="ACJ75751">
        <property type="protein sequence ID" value="ACJ75751"/>
        <property type="gene ID" value="THA_1306"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1306"/>
    <dbReference type="eggNOG" id="COG0787">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028393_2_2_0"/>
    <dbReference type="OMA" id="WEILCGF"/>
    <dbReference type="OrthoDB" id="859043at2"/>
    <dbReference type="UniPathway" id="UPA00042">
        <property type="reaction ID" value="UER00497"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008784">
        <property type="term" value="F:alanine racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030632">
        <property type="term" value="P:D-alanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01201">
        <property type="entry name" value="Ala_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000821">
        <property type="entry name" value="Ala_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011079">
        <property type="entry name" value="Ala_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020622">
        <property type="entry name" value="Ala_racemase_pyridoxalP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00842">
        <property type="entry name" value="Ala_racemase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00992">
        <property type="entry name" value="ALARACEMASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01005">
        <property type="entry name" value="Ala_racemase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00492">
        <property type="entry name" value="alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00395">
        <property type="entry name" value="ALANINE_RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ala_racemase_C" evidence="6">
        <location>
            <begin position="234"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for D-alanine" evidence="3">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for L-alanine" evidence="3">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3 4">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600821-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600821-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="364" mass="40923" checksum="6C17BA6F3E725F2C" modified="2009-02-10" version="1">MGTESRRTFAEININNYIENLKYFQKKCSPAKVMPVIKANAYGHGAVMLAKSAEKMGIDYFAVAFLEEALILRKNGINTNILVFNYLDKDFVPLAADNNITITIISNEQVEEYKDLIKKPKVHINIDTGMNRVGIEPKDAMKIYEKLISNGFEVEGIYTHFAVADSLKKEDIEFTKKQYEKFLEVNLPVKIRHTNNSAASLSGIISCGDYVRIGIASYGLMPSNQVKDENLKPVLTWKSVVSHVKTLKKGESVSYGRTFVAPYDMKVATIPVGYADGYWRHLSNNGEVLINGKRRKILGRVCMDQFVVEADDEVKIGDEVILIGKQGNEFISAEEIADKVGTINYEVTCRISERVPRIYKGVEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICN0</accession>
    <name>RL13_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplM</name>
        <name type="ordered locus">THA_1312</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580142.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ICN0"/>
    <dbReference type="SMR" id="B7ICN0"/>
    <dbReference type="STRING" id="484019.THA_1312"/>
    <dbReference type="EnsemblBacteria" id="ACJ75757">
        <property type="protein sequence ID" value="ACJ75757"/>
        <property type="gene ID" value="THA_1312"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1312"/>
    <dbReference type="eggNOG" id="COG0102">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082184_2_2_0"/>
    <dbReference type="OMA" id="DFVVIIN"/>
    <dbReference type="OrthoDB" id="1822215at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_L13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_L13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_L13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_L13_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="PTHR11545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="SSF52161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000144188" description="50S ribosomal protein L13">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <sequence length="149" mass="17128" checksum="82853C73BB04223B" modified="2009-02-10" version="1">MARTLPIQKTTLLKKEEVKKDWYLINAEGQTLGRLATRIALVLMGKNKPNWTPHVDCGNFVVVINADKIKLTGKKWDQKIYYRHSGYPGGIKEFTARQLQQRNPEKLIQLAVKRMLPKTILGRKALKRLKIYAGSEHPHSAQKPIELNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICP2</accession>
    <name>B7ICP2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1324</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1324"/>
    <dbReference type="EnsemblBacteria" id="ACJ75769">
        <property type="protein sequence ID" value="ACJ75769"/>
        <property type="gene ID" value="THA_1324"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1324"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_92_10_0"/>
    <dbReference type="OMA" id="IRQHAEF"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="3"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="HD-GYP" evidence="3">
        <location>
            <begin position="270"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="462" mass="53367" checksum="74562D420DE808E7" modified="2009-02-10" version="1">MNKIMIIDDDEFVHKYIDEVLSKECEYEIETIHAFSEKQASKLIEKFKDDISVFFIDVFMENDNSGLVLVDLIRNHLSLRKPRIILATSYKEFVEKKHDISDFDISFFMDKQYMDEFMVKHTFLIAIKSYIEIVTLENTIKSLQKIIELSRNKEKFDNILKFLKFMLYEAINLLRFSSNIGIEGFVTLNGIVYAATPKFEEYLNVPLSKVNFTFFPESTIYFEKELAKDLSIKVYIISNIKLKEINKHLVSLFLEEITNSYSSNYYSNLYSQTQKELLAKLLSAIEASSGETGEHVTRVGKIAKILASKINYSQIDEIEIAASIHDLGKIAIPDSILNKPGKLDDEEYAIIKEHPIIGYNILENSTSKILKLAAVICLQHHENWDGSGYPYGLEKEQIDISARITSIADVFDALVSDRIYRKALPLNKALEIIKKERGKKFDPNLVDIFFENLDEILEIYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICQ0</accession>
    <name>B7ICQ0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580151.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1332"/>
    <dbReference type="EnsemblBacteria" id="ACJ75777">
        <property type="protein sequence ID" value="ACJ75777"/>
        <property type="gene ID" value="THA_1332"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1332"/>
    <dbReference type="eggNOG" id="COG0388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_3_1_0"/>
    <dbReference type="OMA" id="NAPYFCQ"/>
    <dbReference type="OrthoDB" id="1583074at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="264" mass="30095" checksum="82A4D1EA4B8A16DB" modified="2009-02-10" version="1">MLIAAAQFSPKPGKFEYNLKKHIDFIEKAGEIGASLILFPELSISGYTYDKNILEDSISFFNEVQSELLKLSRKYNMAIVGGVPRKVLSEVRNSVFVIKKKKEILFYDKTHLFRGEKDVFLPGERFLVFKFQGVRFGILICYEIGFPEISRVLALNGAQVLLAPFAFSKERKNIYEIATRARALENGAFLVTSSTSGKGLMDFIGSSRIVGPDGNVITQARRKEELIYADLDVSKLDFFRYKEEGISHAYFLNRKKELYRSVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICR6</accession>
    <name>B7ICR6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1348</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580165.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1348"/>
    <dbReference type="EnsemblBacteria" id="ACJ75793">
        <property type="protein sequence ID" value="ACJ75793"/>
        <property type="gene ID" value="THA_1348"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1348"/>
    <dbReference type="eggNOG" id="COG2227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098947_0_0_0"/>
    <dbReference type="OMA" id="DHRAWFS"/>
    <dbReference type="OrthoDB" id="957193at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="236" mass="27341" checksum="D08E5AD9208D1674" modified="2009-02-10" version="1">MSNNAKSNLTDFLSGKLFSNGLEISIQESPYFLRTELLEKICESKKIIHIGFADHKDLIIKKIENNVWLHGRILKKAAKCVGIDINKEAIEFIQKKIGIKDLYCLDVEKDDLPLEILSEKWDYILIPEVIEHVDNPVNFLKNIKNKFSNISDKIVITVPNAFKFENFLNAMKNKEVINSDHRYWFTVYTIAKVLTLSGISIENFYLANSYHNSAFYEITKKSPLFRDDIIVIGKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICT0</accession>
    <name>B7ICT0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Asparagine--tRNA ligase</fullName>
            <ecNumber evidence="5">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Asparaginyl-tRNA synthetase</fullName>
            <shortName evidence="5">AsnRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">asnS</name>
        <name evidence="7" type="ordered locus">THA_1362</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + H(+) + L-asparaginyl-tRNA(Asn)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580178.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1362"/>
    <dbReference type="EnsemblBacteria" id="ACJ75807">
        <property type="protein sequence ID" value="ACJ75807"/>
        <property type="gene ID" value="THA_1362"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1362"/>
    <dbReference type="eggNOG" id="COG0017">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_0_0"/>
    <dbReference type="OMA" id="DNMDLAE"/>
    <dbReference type="OrthoDB" id="1138123at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00534">
        <property type="entry name" value="Asn_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="6">
        <location>
            <begin position="133"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="431" mass="50095" checksum="27CF09F0CB99822E" modified="2009-02-10" version="1">MWVYIKNLKDYIGKEVELRGWVWRSRSSGKIAFINMRDGTGIVQVVVEASSVDEETFKNARKLRMESSLIVRGTVKSEERAPGGVEIHAKEVIPVQIPTEDFPINKPDHSIDYLMEHRHLWLRSRRQMHILNIRDAVLRAIRKFYWENDFVQVDTPIFTGAIGESAGNLFEIDYFDYGKVYLAQTGQLYLEAACMALGRVFNLGPTFRAEKSKTRRHLIEFWMNEAEVAYFEHEDNLKLQENLVSYIVKYVLENAREHLEALNRDISKLEKIEPPFPRITYTEAVKMLQKKGYDIEWGDDFGGDEETEIAKQFDKPVFVTHYPRKAKAFYMQPDPDNPDVVLCDDLIAPEGYGEIIGASQRIHDYDLLVERLKEFNLPVESYDWYLDLRKFGSVPHSGFGLGVERTIAWIAGLEHIREAIPFPRTLYRVHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICT9</accession>
    <name>B7ICT9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580187.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1371"/>
    <dbReference type="EnsemblBacteria" id="ACJ75816">
        <property type="protein sequence ID" value="ACJ75816"/>
        <property type="gene ID" value="THA_1371"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1371"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1905777_0_0_0"/>
    <dbReference type="OrthoDB" id="1538300at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="164" mass="19662" checksum="85D9AAFC8F805D39" modified="2009-02-10" version="1">MESNEKTLKLTRISDEELKNFLDMKFKDFSAEEVNFDLFKDEVNTLTFQIEFKGDSAGIAQFYNIDWVNRSACIRIFWDEAIENDVEVKVVRLMLKYAFEVLNLNRVYSKVVEYKKDTISVLKKCGFLQEGMERQAKFFKGKYWDVLRFSMLYEDYWRAIDDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID33</accession>
    <name>B7ID33_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoesterase</fullName>
            <ecNumber evidence="3">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1467</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580243.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1467"/>
    <dbReference type="EnsemblBacteria" id="ACJ75910">
        <property type="protein sequence ID" value="ACJ75910"/>
        <property type="gene ID" value="THA_1467"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1467"/>
    <dbReference type="eggNOG" id="COG0622">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063749_1_1_0"/>
    <dbReference type="OMA" id="DILYHGP"/>
    <dbReference type="OrthoDB" id="1355703at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00841">
        <property type="entry name" value="MPP_YfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041802">
        <property type="entry name" value="MPP_YfcE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="PTHR11124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="4">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="184" mass="21050" checksum="858159B33087D7EF" modified="2009-02-10" version="1">MKVLVISDTHGSVYYWNKIKKIAEQVDEIFHLGDVLYHGPRNPLPEGYNPKELVEELKKFNINYIRGNCDADVDVKVLGLQEMPKKTMEFFKDIPIYLFHGESIENDNFDVTSFAKNHNVRIILHGHTHIPKIEEKEGIIIANPGSLSLPKGGFPQTYMIIDLSNTPKITIYDLDNKEVLSKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID67</accession>
    <name>B7ID67_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580259.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1503"/>
    <dbReference type="EnsemblBacteria" id="ACJ75944">
        <property type="protein sequence ID" value="ACJ75944"/>
        <property type="gene ID" value="THA_1503"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1503"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_10_1_0"/>
    <dbReference type="OMA" id="DFYTAMI"/>
    <dbReference type="OrthoDB" id="1860081at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="120" mass="13675" checksum="A4A37245C11BB8F0" modified="2009-02-10" version="1">MLRPVDKHSGIPSYIQIVNMIKAEILLGKINPGEQLLPVRKLKEIFQVNINTVLKALEKLKLEGYITSEQGIGYFVSKELQIAPTIMAEVKEAIKKLKSTKIDLNTLFIILEEVWKDEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID71</accession>
    <name>B7ID71_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1507</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580261.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1507"/>
    <dbReference type="EnsemblBacteria" id="ACJ75948">
        <property type="protein sequence ID" value="ACJ75948"/>
        <property type="gene ID" value="THA_1507"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1507"/>
    <dbReference type="eggNOG" id="COG1413">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103695_0_0_0"/>
    <dbReference type="OMA" id="GENCESK"/>
    <dbReference type="OrthoDB" id="1617484at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="204" mass="23557" checksum="093AE27C79BB787A" modified="2009-02-10" version="1">MEREELIKRIIEEKGEQAIDDLIKLLEDEDSQVKEIASETLFKLGKASKDPLYREFKKRIHEGNRNDITLLYIIDLLADLEDERIKKDLYNALSLFDFEEAELIIYEALSKLGDGEKVYKILRYFLLEDADRKKYGAQAAMALSYIEMPEVVKDLVQAIDSGDFSGEDLEIIKKALSNLVTRNSMYYEILKKLVGDKDEDYLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID74</accession>
    <name>B7ID74_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Uridine kinase</fullName>
            <ecNumber evidence="4">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1510</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + cytidine = ADP + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + uridine = ADP + H(+) + UMP</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102032.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1510"/>
    <dbReference type="EnsemblBacteria" id="ACJ75951">
        <property type="protein sequence ID" value="ACJ75951"/>
        <property type="gene ID" value="THA_1510"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1510"/>
    <dbReference type="eggNOG" id="COG0572">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021278_1_2_0"/>
    <dbReference type="OMA" id="TTLKPMH"/>
    <dbReference type="OrthoDB" id="1793376at2"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PRK" evidence="5">
        <location>
            <begin position="3"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="204" mass="23650" checksum="15063215A8F22CED" modified="2009-02-10" version="1">MFIIGIGGGTGSGKTTVAQKINEIIGESNSVILPMDNYYKDMSHVPFEERKKYNYDHPDMIEFSLLVDHLKELLDGKSIKLPEYDFAQYTRTGNFTLLDPKPVIIIEGIFALYYEELRKFYDLSIFVDAESDVRFIRRLERDIKERGRTIDSVINQYLNMVKPMHDAYVEPSKKYADLIIPRGGFNEKAINVVVEFIFKKMSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDA4</accession>
    <name>B7IDA4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580267.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1540"/>
    <dbReference type="EnsemblBacteria" id="ACJ75981">
        <property type="protein sequence ID" value="ACJ75981"/>
        <property type="gene ID" value="THA_1540"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1540"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2048606_0_0_0"/>
    <dbReference type="OrthoDB" id="1602420at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="SSF49265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="1">
        <location>
            <begin position="36"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="120" mass="13008" checksum="7CEE130905358408" modified="2009-02-10" version="1">MKRSSTLLAILVFLLMILIFSCTNPSPKIINHPPSIPNNPQPLDVATGVVLNPTLSWTCTDPNGDTLTYDVYFSTDATPCLAATNLTANTYNPGTLEKNTTYYWKVVAQDNKGGTSESPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDB2</accession>
    <name>B7IDB2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1550</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580272.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1550"/>
    <dbReference type="EnsemblBacteria" id="ACJ75989">
        <property type="protein sequence ID" value="ACJ75989"/>
        <property type="gene ID" value="THA_1550"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1550"/>
    <dbReference type="eggNOG" id="COG0535">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044700_0_0_0"/>
    <dbReference type="OMA" id="YADKLWY"/>
    <dbReference type="OrthoDB" id="1172757at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="106"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="482" mass="55910" checksum="058B698A69161AAC" modified="2009-02-10" version="1">MAVGGIKGMIYRQAGKMINSFVRKADTQSFAKLLFTVGALSKEPAKSGLKKLGLMAQEEHPMIKKWIEIFQKSSPKCTEKIINNLIINEWAIGEPLRQKNMENEKVVLPKLGVISPTYACNLNCIGCYAGLYGRKYELSKEEVRSILKQGEELGIFFWIITGGEPFYWPHLLEILEEFDQHYFMIYSNGILINEEKAKKLSELGNATIAISVEGFEEETDWRRGKGVFNAIQNTWERLRRYGVPFGASVTATRKNHDVIMKDEFWDFLAENGVAYTWVFQFMPVGMNPSMDLVPTPKQRYERFFKTDEVRLSGRFAFVADFWNHGFLTHGCLSAGSKYFHVNAKGYVEPCVFQQFAKDSIREKTLLEILKSPFFEAYKRMIPFSNNLFRPCPIIDNPKVFRAMVKHFDAIPQHEGSEKVITELAPNIDKLAEEWKQYADKLWYEHGYVERYPVNRGIYNYETRMKRYANREEALKVDKKLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDB5</accession>
    <name>B7IDB5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polyribonucleotide nucleotidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1553</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102227.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1553"/>
    <dbReference type="EnsemblBacteria" id="ACJ75992">
        <property type="protein sequence ID" value="ACJ75992"/>
        <property type="gene ID" value="THA_1553"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1553"/>
    <dbReference type="eggNOG" id="COG1098">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128762_0_1_0"/>
    <dbReference type="OMA" id="SFMRDAK"/>
    <dbReference type="OrthoDB" id="1235756at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035104">
        <property type="entry name" value="Ribosomal_protein_S1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10724:SF7">
        <property type="entry name" value="PTHR10724:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="4"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="124" mass="14153" checksum="CC92D2847837DC75" modified="2009-02-10" version="1">MEVGQVVKGKVVEILKFGANIELEDGEKAFVHISKIAPTYVKSVSDHLSLGQEIEGKIVGKTRDGKWELTLKDTSANSSEGKSEEKKVNNDFEKKLAKFMKDSDRKLSEYRRRLDKKRGKKERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDB8</accession>
    <name>B7IDB8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="13">NADP-reducing hydrogenase, subunit c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">THA_1556</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580274.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1556"/>
    <dbReference type="EnsemblBacteria" id="ACJ75995">
        <property type="protein sequence ID" value="ACJ75995"/>
        <property type="gene ID" value="THA_1556"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1556"/>
    <dbReference type="eggNOG" id="COG1894">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014881_3_2_0"/>
    <dbReference type="OMA" id="CDDVIMD"/>
    <dbReference type="OrthoDB" id="688908at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="SSF140490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="SSF142019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="540"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="570"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ75995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="602" mass="65620" checksum="8829BEC95293FA83" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDF1</accession>
    <name>B7IDF1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Desulfoferrodoxin</fullName>
            <ecNumber evidence="5">1.15.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Superoxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">THA_1590</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the one-electron reduction of superoxide anion radical to hydrogen peroxide at a nonheme ferrous iron center. Plays a fundamental role in case of oxidative stress via its superoxide detoxification activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 H(+) + reduced [rubredoxin] + superoxide = H2O2 + oxidized [rubredoxin]</text>
            <dbReference type="Rhea" id="RHEA:21324"/>
            <dbReference type="Rhea" id="RHEA-COMP:10302"/>
            <dbReference type="Rhea" id="RHEA-COMP:10303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.15.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the desulfoferrodoxin family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.2" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102338.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1590"/>
    <dbReference type="EnsemblBacteria" id="ACJ76028">
        <property type="protein sequence ID" value="ACJ76028"/>
        <property type="gene ID" value="THA_1590"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1590"/>
    <dbReference type="eggNOG" id="COG2033">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118960_1_0_0"/>
    <dbReference type="OMA" id="EEKHYIQ"/>
    <dbReference type="OrthoDB" id="1496450at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050605">
        <property type="term" value="F:superoxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00974">
        <property type="entry name" value="DSRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002742">
        <property type="entry name" value="Desulfoferrodoxin_Fe-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036073">
        <property type="entry name" value="Desulfoferrodoxin_Fe-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004462">
        <property type="entry name" value="Desulfoferrodoxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038094">
        <property type="entry name" value="Desulfoferrodoxin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004793">
        <property type="entry name" value="Desulfoferrodoxin_rbo"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06397">
        <property type="entry name" value="Desulfoferrod_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01880">
        <property type="entry name" value="Desulfoferrodox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49367">
        <property type="entry name" value="SSF49367"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00319">
        <property type="entry name" value="desulf_FeS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00320">
        <property type="entry name" value="dfx_rbo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00332">
        <property type="entry name" value="neela_ferrous"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Desulfoferrod_N" evidence="14">
        <location>
            <begin position="2"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Desulfoferrodox" evidence="13">
        <location>
            <begin position="42"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ACJ76028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="126" mass="14499" checksum="C1E32B32DBC2C0BD" modified="2009-02-10" version="1">MTKRIQVYKCEKCGNIVEVLHEGAGTLVCCGEPMKLLEEKTADTATEKHVPFIEELEDGYRVRVGENALHPMEEKHYIEWIELIVDGVVYRKFLNPGDKPEAEFKVAKGKEVHAREYCNVHGLWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDJ9</accession>
    <name>B7IDJ9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Flagellar protein FliL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fliL</name>
        <name evidence="9" type="ordered locus">THA_1638</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Controls the rotational direction of flagella during chemotaxis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the FliL family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580316.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1638"/>
    <dbReference type="EnsemblBacteria" id="ACJ76076">
        <property type="protein sequence ID" value="ACJ76076"/>
        <property type="gene ID" value="THA_1638"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1638"/>
    <dbReference type="eggNOG" id="COG1580">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1545329_0_0_0"/>
    <dbReference type="OMA" id="DALMMIF"/>
    <dbReference type="OrthoDB" id="1767204at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005503">
        <property type="entry name" value="FliL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03748">
        <property type="entry name" value="FliL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="174" mass="18972" checksum="C3C12AB8A357BD7F" modified="2009-02-10" version="1">MPEEEIQEGQQPKKKKGIMGLLGPILIPLVISLVVSVAVVMFLGNNTQVQTQKTTTTTTTTAVPIKAVVIQTGTYQTFMLKGGKEVAVIDSLSFKVGSDQCRSLIAEKNDEIMDALMLIFLSKERTEINTPAGIELLKKQIKNAVNEITGFVGEKEKEGVIDVYLYIKAVSSVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDM4</accession>
    <name>B7IDM4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Medium FAD-binding subunit of molybdenum enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102474.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1663"/>
    <dbReference type="EnsemblBacteria" id="ACJ76101">
        <property type="protein sequence ID" value="ACJ76101"/>
        <property type="gene ID" value="THA_1663"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1663"/>
    <dbReference type="eggNOG" id="COG1319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058050_0_0_0"/>
    <dbReference type="OMA" id="MVEINFD"/>
    <dbReference type="OrthoDB" id="1017413at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03450">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="SSF55447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="280" mass="31886" checksum="FF9503FB8D66D841" modified="2009-02-10" version="1">MIKNYFLPKTVEELSEIKASTKGFLFSGGTDLFVKLRANAINTDTVIDTKAIIETPNISDKLFIPLNFTYSQLREYLKDNNINKYLDNIIRLIGSPMIRNRGTPVGNIANASPAGDFVLASYLLNAKVIVKPTNKKIPIEDFIKGPGKIELEENEFIFGVELDIKNDYKFYFEKVGRRNAMIISIASIAILLKESNNKIDDISICYGSVGPTILREKLLEEKVKGKEISLELFEYLASEYQKLSNPITDVRASKEYRKQLVYNLMIKAYYNLKEKKVMNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDP8</accession>
    <name>B7IDP8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH dehydrogenase subunit n</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1688</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580350.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1688"/>
    <dbReference type="EnsemblBacteria" id="ACJ76125">
        <property type="protein sequence ID" value="ACJ76125"/>
        <property type="gene ID" value="THA_1688"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1688"/>
    <dbReference type="eggNOG" id="COG0651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_9_5_0"/>
    <dbReference type="OMA" id="PPLWWGV"/>
    <dbReference type="OrthoDB" id="1887859at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="589"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="3">
        <location>
            <begin position="121"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="614" mass="68892" checksum="38C93B494C05BE88" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDS9</accession>
    <name>B7IDS9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Glutamine-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="5 6">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nadE2</name>
        <name evidence="5" type="synonym">nadE</name>
        <name evidence="9" type="ordered locus">THA_1720</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>ATP + deamido-NAD(+) + H2O + L-glutamine = AMP + diphosphate + H(+) + L-glutamate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:24384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 6">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="5 6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102586.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1720"/>
    <dbReference type="EnsemblBacteria" id="ACJ76156">
        <property type="protein sequence ID" value="ACJ76156"/>
        <property type="gene ID" value="THA_1720"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1720"/>
    <dbReference type="eggNOG" id="COG0171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022313_2_0_0"/>
    <dbReference type="OMA" id="RLAQDCY"/>
    <dbReference type="OrthoDB" id="1152435at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00334"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02090">
        <property type="entry name" value="NadE_glutamine_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="PTHR23090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006630">
        <property type="entry name" value="NADS_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 6 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="8">
        <location>
            <begin position="3"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for glutaminase activity" evidence="5">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="active site" description="For glutaminase activity" evidence="5">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutaminase activity" evidence="5">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="322"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="546"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="576" mass="65981" checksum="E97A57C1A2004CA9" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDU1</accession>
    <name>TGT_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Queuine tRNA-ribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Guanine insertion enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tgt</name>
        <name type="ordered locus">THA_1733</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-aminomethyl-7-carbaguanine + guanosine(34) in tRNA = 7-aminomethyl-7-carbaguanosine(34) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:24104"/>
            <dbReference type="Rhea" id="RHEA-COMP:10341"/>
            <dbReference type="Rhea" id="RHEA-COMP:10342"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:58703"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82833"/>
            <dbReference type="EC" id="2.4.2.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the queuine tRNA-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.29" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580376.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDU1"/>
    <dbReference type="SMR" id="B7IDU1"/>
    <dbReference type="STRING" id="484019.THA_1733"/>
    <dbReference type="EnsemblBacteria" id="ACJ76168">
        <property type="protein sequence ID" value="ACJ76168"/>
        <property type="gene ID" value="THA_1733"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1733"/>
    <dbReference type="eggNOG" id="COG0343">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022060_0_1_0"/>
    <dbReference type="OMA" id="GIDLFDC"/>
    <dbReference type="OrthoDB" id="1165356at2"/>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008479">
        <property type="term" value="F:queuine tRNA-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101030">
        <property type="term" value="P:tRNA-guanine transglycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00168">
        <property type="entry name" value="Q_tRNA_Tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004803">
        <property type="entry name" value="TGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="SSF51713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00430">
        <property type="entry name" value="Q_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000198033" description="Queuine tRNA-ribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding; important for wobble base 34 recognition" evidence="1">
        <location>
            <begin position="270"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="91"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00168"/>
        </source>
    </evidence>
    <sequence length="367" mass="41218" checksum="6E7C1E9B32BB8537" modified="2009-02-10" version="1">MGLKFELLKTSGNARRGRIYLPHGVVETPTFMPVGTNANVKLMTPKLLDEIGAQIILGNAFHLYLKPGLDVFRFHGGIHNFMNWQKPVLTDSGGFQVFSLREGRKITEDGVLIRSPLDGSKHMITPELSMEIQHAIGSDIVMAFDYCAEPGISHQDAVVALELTSLWAERSLKKLRSLSDQAIFGIVQGAFFKDLRLRSAKEITSMNFDGFAIGGLSVGEEYDITLDMTKFTAPLLPENKPRYFMGAGSPKLIVDLVDSGIDMFDSVLPTRVARHGQALTWKGKLNIRSAKYKFSKEPIDESCGCYTCKNFSRSYIRHLFDRGEVLGQILLTIHNLHFMMDLSKKIRESIENDTFQELRGEVLKYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDV4</accession>
    <name>B7IDV4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102644.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1747"/>
    <dbReference type="EnsemblBacteria" id="ACJ76181">
        <property type="protein sequence ID" value="ACJ76181"/>
        <property type="gene ID" value="THA_1747"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1747"/>
    <dbReference type="HOGENOM" id="CLU_2304708_0_0_0"/>
    <dbReference type="OMA" id="IELHPIY"/>
    <dbReference type="OrthoDB" id="2069719at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="102" mass="11810" checksum="D7B492027F41C5FB" modified="2009-02-10" version="1">MDPIELHPIYTRASVEKSAHFSNLQNYANVLSEINANQLKEKIKREQSSPKVVERTSSNLIKNSTEKNESKNNQFYNKEGKKNKNKEDIVKAENNHILDVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDY2</accession>
    <name>B7IDY2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit biogenesis GTPase RsgA</fullName>
            <ecNumber evidence="7">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 12" type="primary">rsgA</name>
        <name evidence="12" type="ordered locus">THA_1778</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Helps release RbfA from mature subunits. May play a role in the assembly of ribosomal proteins into the subunit. Circularly permuted GTPase that catalyzes slow GTP hydrolysis, GTPase activity is stimulated by the 30S ribosomal subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer. Associates with 30S ribosomal subunit, binds 16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class YlqF/YawG GTPase family. RsgA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580403.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1778"/>
    <dbReference type="EnsemblBacteria" id="ACJ76209">
        <property type="protein sequence ID" value="ACJ76209"/>
        <property type="gene ID" value="THA_1778"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1778"/>
    <dbReference type="eggNOG" id="COG1162">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033617_2_1_0"/>
    <dbReference type="OMA" id="CLVAAYD"/>
    <dbReference type="OrthoDB" id="908180at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04466">
        <property type="entry name" value="S1_YloQ_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01854">
        <property type="entry name" value="YjeQ_EngC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01820">
        <property type="entry name" value="GTPase_RsgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004881">
        <property type="entry name" value="Ribosome_biogen_GTPase_RsgA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010914">
        <property type="entry name" value="RsgA_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031944">
        <property type="entry name" value="RsgA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32120">
        <property type="entry name" value="PTHR32120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03193">
        <property type="entry name" value="RsgA_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16745">
        <property type="entry name" value="RsgA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00157">
        <property type="entry name" value="TIGR00157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50936">
        <property type="entry name" value="ENGC_GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="S1-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="CP-type G" evidence="11">
        <location>
            <begin position="66"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="ENGC_GTPASE" evidence="10">
        <location>
            <begin position="75"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="115"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="165"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ76209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="287" mass="32739" checksum="8F517979A31151C8" modified="2009-02-10" version="1">MRRKGVVIKFLSNMAIVQDFETGEEFLCKLRGKFKEQKIRPIVGDIVEYSPIVSKEGVIENILNRKNELPRPKIANIDQVVVVTSLRKPEVPLQILDKYLVLVENAKLPAVIVLNKIDLLTKEEINKFKYIYEKIYPVYFVSAKTKEGIEELKEVFKNKISTMAGMSGVGKSSILNAINPGLSLKVNDVSEKLDRGRHTTTVIELMHFDFGGWIADTPGFANLDISHIEHEELKRLFPEFNDFYCLFPDCNHVDEPGCGVKKAVEEGKISKTRYESYLQIYKQLLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE09</accession>
    <name>B7IE09_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1805</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580429.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1805"/>
    <dbReference type="EnsemblBacteria" id="ACJ76236">
        <property type="protein sequence ID" value="ACJ76236"/>
        <property type="gene ID" value="THA_1805"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1805"/>
    <dbReference type="eggNOG" id="ENOG5033BU5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187578_0_0_0"/>
    <dbReference type="OMA" id="YNCKIWI"/>
    <dbReference type="OrthoDB" id="2036885at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="82" mass="9806" checksum="2DEF3A7A327C9E2E" modified="2009-02-10" version="1">MSDCNLKSILEKIARKYNCKIWINKKIGRRISFIEKAGNEYYLPPMVVYEDDKFIIFVENLKRSDEEFKKLIQEVVDCARNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE47</accession>
    <name>B7IE47_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Endonuclease/Exonuclease/phosphatase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102813.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1843"/>
    <dbReference type="EnsemblBacteria" id="ACJ76274">
        <property type="protein sequence ID" value="ACJ76274"/>
        <property type="gene ID" value="THA_1843"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1843"/>
    <dbReference type="eggNOG" id="COG3568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065532_0_0_0"/>
    <dbReference type="OMA" id="YMNKHYE"/>
    <dbReference type="OrthoDB" id="1387978at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="354" mass="40873" checksum="F659D7DA50F2E222" modified="2009-02-10" version="1">MKYIKLTLILLISIALVIAGYFGILILIGTLTDYKPEKIEKIEVENVSTLTIDSSRLTIFDWNIGYGGLGKDMDFFMDGGKKAITDKNDVLKNLEGIKEILIKNEADIYLIQEIDRNSRRSGYVDELDYLKENVFKNYNIAFAYNYKVLFVPVPYLNPTGKIEAGLATFSKYKIDEALRMALPGEYEWPTRLFQLDRCLILTRMKVKNGKDLVILNIHPSAYDPGGKLREQQLKFIMETAKKEFELGNYVIIGGDWNSEFFDNLDFKYTEEKPESYIKLPDFFKLEGWKWAIDNTVPTNRSLKTKYIPGQSFVTVIDGFYVSPNIEIDYVKNIDLNFEFTDHNPVILKITLVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE50</accession>
    <name>B7IE50_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1846</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580459.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1846"/>
    <dbReference type="EnsemblBacteria" id="ACJ76277">
        <property type="protein sequence ID" value="ACJ76277"/>
        <property type="gene ID" value="THA_1846"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1846"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_896960_0_0_0"/>
    <dbReference type="OMA" id="ISYSAVI"/>
    <dbReference type="OrthoDB" id="1581914at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="20"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="310" mass="36531" checksum="4A490788A78FA3F7" modified="2009-02-10" version="1">MKIFVKWIFKMPKKYLCSLFGLSFLSVLSTIFISYSSILSRDLINKAALKNENYVYYAILFFLAVLVSHIFLLFQRCLSYYFIGRYLNFYGTYCYKKIMSKDYLNFSKKTSSFYSQISFRTVKDMINFVSNNESTDGVVFALKIFTYISAMYYIDKLSGIYMIIFSILILITLAISNSFYYKNLKQLNDEFLEIKSYVADTFSGIQEIKLFNAKKAEFENYKKRTNYYWKKTKSVYIIDQILSNFSRNLFSMIFYFLVIKQGINLKDPGSFFALTNLFLLLRYQLFTSLGVWDNIRTAVISAKQLEENVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE62</accession>
    <name>B7IE62_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">M18 family aminopeptidase</fullName>
            <ecNumber evidence="10">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">THA_1863</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M18 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102853.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1863"/>
    <dbReference type="MEROPS" id="M18.004"/>
    <dbReference type="EnsemblBacteria" id="ACJ76289">
        <property type="protein sequence ID" value="ACJ76289"/>
        <property type="gene ID" value="THA_1863"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1863"/>
    <dbReference type="eggNOG" id="COG1362">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_590123_0_0_0"/>
    <dbReference type="OMA" id="GPILKVN"/>
    <dbReference type="OrthoDB" id="304020at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023358">
        <property type="entry name" value="Peptidase_M18_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="PTHR28570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00932">
        <property type="entry name" value="AMINO1PTASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ76289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="445" mass="49762" checksum="D06F00FDEF4E8123" modified="2009-02-10" version="1">MSLWKKRNLEEVEKFSKDYRKFIDEAVSERLAVDFFENLLIENGFIPLDKYTGKEEKVFYTNANKSLVAARIINKPEDGFNIIAAHIDAPRIDLKPNPLYEDEGFALMKTHYYGGVRLYQWLNIPLALVGVVYKDDGTKLNIKIGFEENDPVFVIADLLPHLDRKDEKISEKFQGEKMNLIVGSIPLDGEEKEAVKKNVLKILKDLYNIEEEDFVSADIELVPAFKSREVGFDKSLIAAYGHDDRICAYQAIRALIDAENLEKSAAVILFDREEIGSEGNAGAKARFYKKFFRKILKINGSNDTESSLDELIENSFVVSADVGALINPTYKDVHDKYNAAKVGEGIVLVKYTGSRGKAGASEAHAEVVAHVRKVLNSADIKWQVGTLGKIGIGGGGTVAKFLAEEGFNVIDMGPGLLSMHSPYELVSKVDLYETYLAYKELISKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE73</accession>
    <name>NADK_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD kinase</fullName>
            <ecNumber evidence="1">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadK</name>
        <name type="ordered locus">THA_1874</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + NAD(+) = ADP + H(+) + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102873.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IE73"/>
    <dbReference type="SMR" id="B7IE73"/>
    <dbReference type="STRING" id="484019.THA_1874"/>
    <dbReference type="EnsemblBacteria" id="ACJ76300">
        <property type="protein sequence ID" value="ACJ76300"/>
        <property type="gene ID" value="THA_1874"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1874"/>
    <dbReference type="eggNOG" id="COG0061">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008831_0_3_0"/>
    <dbReference type="OMA" id="VNLGHVG"/>
    <dbReference type="OrthoDB" id="1463423at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000120892" description="NAD kinase">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="53"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="117"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="157"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <sequence length="255" mass="28513" checksum="E126E15FB5B6751A" modified="2009-02-10" version="1">MKHLGIFYKPELFGKAESLKELLLNEGYIVDYCESSERIKNNKVDLTIVLGGDGTFLKASHLVNNPLVGFKGGRLGFLSSYTVEEFDKFLKDLKNNNFVSDERTFLKVSDLNTFCLNEVLLVKDPDQKMVDIKISFQDGELFFHADGIMLSTPTGSTGYSLSLGGPILLPNTKAFVITPVAPQFLASRSIVIPDNEKVNIEVDKRVNLIIDGVNFGKFSKITVMKSKKKISILRPVDYDFTKSIKEKLGYGKKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE90</accession>
    <name>B7IE90_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Rrf2 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1891</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102897.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1891"/>
    <dbReference type="EnsemblBacteria" id="ACJ76317">
        <property type="protein sequence ID" value="ACJ76317"/>
        <property type="gene ID" value="THA_1891"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1891"/>
    <dbReference type="eggNOG" id="COG1959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107144_1_1_0"/>
    <dbReference type="OMA" id="LRMLMYC"/>
    <dbReference type="OrthoDB" id="1794390at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030489">
        <property type="entry name" value="TR_Rrf2-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00738">
        <property type="entry name" value="rrf2_super"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01332">
        <property type="entry name" value="HTH_RRF2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="148" mass="16868" checksum="BD85191F216EC225" modified="2009-02-10" version="1">MAVTMKSDYALRLLLLLSIENKRMSTSELISKCKTSIPYEFAQKILSQLVASGILESSRGKFGGYKLVKDPKDITVYDIVTSVDDISSMITCFVPPDKISLAPEICSVNEVWEIVMKRFKQSLKSVTLEDLKESYLKKCEEFERRQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE94</accession>
    <name>B7IE94_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease V</fullName>
            <ecNumber evidence="1">3.1.21.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Deoxyinosine 3'endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Deoxyribonuclease V</fullName>
            <shortName evidence="1">DNase V</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nfi</name>
        <name evidence="2" type="ordered locus">THA_1895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate.</text>
            <dbReference type="EC" id="3.1.21.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the endonuclease V family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.7" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102905.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1895"/>
    <dbReference type="EnsemblBacteria" id="ACJ76321">
        <property type="protein sequence ID" value="ACJ76321"/>
        <property type="gene ID" value="THA_1895"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1895"/>
    <dbReference type="eggNOG" id="COG1515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047631_1_1_0"/>
    <dbReference type="OMA" id="RIHFRQA"/>
    <dbReference type="OrthoDB" id="1681607at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043737">
        <property type="term" value="F:deoxyribonuclease V activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06559">
        <property type="entry name" value="Endonuclease_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00801">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007581">
        <property type="entry name" value="Endonuclease-V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511">
        <property type="entry name" value="PTHR28511"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04493">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with target DNA" evidence="1">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="221" mass="25266" checksum="35BDFFE2A64C5616" modified="2009-02-10" version="1">MIHRWDVTPKEAISIQKSLNDKLIFKPFNKTPTYVAGVDLSYVNNKSLAVIVVIDSKFNVVEITYHIDKIKYPYIPGLLAFREGPVFLKAWQKIKTNVDLVMFDGHGIAHPRKLGIASHMGLWIKKPTIGIAKKKLVGTYDEPSNTKFSSSYLWYNGEKIGIVLKSRENTKPIFISPGHLIDLDSSLNIVKKYITKYKLPEPTRLAHIYTQKLKNKIIEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE99</accession>
    <name>B7IE99_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Folylpolyglutamate synthase/dihydrofolate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1900</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1900"/>
    <dbReference type="EnsemblBacteria" id="ACJ76326">
        <property type="protein sequence ID" value="ACJ76326"/>
        <property type="gene ID" value="THA_1900"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1900"/>
    <dbReference type="eggNOG" id="COG0285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015869_1_1_0"/>
    <dbReference type="OMA" id="YFEMGTL"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="PTHR11136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001563">
        <property type="entry name" value="Folylpolyglu_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="4">
        <location>
            <begin position="45"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR001563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="420" mass="48168" checksum="D19C2C38CA120DE1" modified="2009-02-10" version="1">MEYLDALKYLYFKRPYGKIKIGLYRIEELLKRLNNPEKNYKCFHITGSNGKGSTTTFLHYLAKNHGYKVGGYLSPHLSSILERFPINGKYISKERFLNAFNLVKKYSEEMDKKGEEFSPSFFEFATALAFEIDKEENVEISTIEVGLGGRFDATNVIIPEVSIITTVSLEHTKILGNSLEKIAYEKAGIIKENRPVVIGNVPKEAKKVIYNIAADKNAKVYELGKDFDYEVVEYQFGKNIINYYGETTIKGLEVNLNGTHQPINAAIALKAYELFEKIDLNNTFVAFKEAFIPGRFEMFNGFLLDGSHNPQAAEKFLENLNIYFKNDSKIALFGILDDKDKISVISKLIPEFEHVIVTCPPSHRAINCLETYELVKDFSKSCEFIDDPIHAIEKLKEMEADLKVVNWFILSCWFHKKLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IED5</accession>
    <name>B7IED5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase YdhF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103010.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1940"/>
    <dbReference type="EnsemblBacteria" id="ACJ76362">
        <property type="protein sequence ID" value="ACJ76362"/>
        <property type="gene ID" value="THA_1940"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1940"/>
    <dbReference type="eggNOG" id="COG4989">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_8_0_0"/>
    <dbReference type="OMA" id="VVYAWVM"/>
    <dbReference type="OrthoDB" id="1367386at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="8"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="296" mass="34324" checksum="1986EABF11A9B5BE" modified="2009-02-10" version="1">MRVELSRVIHGLMRIKSWKYTTEELLSLIYKLIDLGVTTFDLADIYGDYEAQEIFGRVLEKDPSLRKKIQLISKVGIVLLSNKHPNVYVKHYDTSKKHIIESVEKTLKDLRTDYIDVLLIHRPDPLMDVEEISGAFEKLKERGMVLSFGVSNFYPSQMSLFVEKLSLPLLYNQIEISLWNVDSFFNGTLDYCQEKGILPMAWSPVAGGRLFKEEDIRKDDRVLRIHNALVEVAKNHYTTPEHIAYAWLYKHPSKIYPVVGSGNFERIKTAVEALNIELDRQEWFYLLKASRGIDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IED6</accession>
    <name>B7IED6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1941</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103012.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="SMR" id="B7IED6"/>
    <dbReference type="STRING" id="484019.THA_1941"/>
    <dbReference type="EnsemblBacteria" id="ACJ76363">
        <property type="protein sequence ID" value="ACJ76363"/>
        <property type="gene ID" value="THA_1941"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1941"/>
    <dbReference type="eggNOG" id="COG3459">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_277846_0_0_0"/>
    <dbReference type="OMA" id="PGWLENE"/>
    <dbReference type="OrthoDB" id="36122at2"/>
    <dbReference type="BRENDA" id="2.4.1.20">
        <property type="organism ID" value="17371"/>
    </dbReference>
    <dbReference type="BRENDA" id="2.4.1.49">
        <property type="organism ID" value="17371"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="1019" mass="119419" checksum="E6DAD62B48698B78" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEG9</accession>
    <name>B7IEG9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoesterase</fullName>
            <ecNumber evidence="2">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1975</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1975"/>
    <dbReference type="EnsemblBacteria" id="ACJ76396">
        <property type="protein sequence ID" value="ACJ76396"/>
        <property type="gene ID" value="THA_1975"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1975"/>
    <dbReference type="eggNOG" id="COG0639">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074761_0_1_0"/>
    <dbReference type="OMA" id="DEYLFPW"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011152">
        <property type="entry name" value="Pesterase_MJ0912"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000883">
        <property type="entry name" value="Pesterase_MJ0912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="3">
        <location>
            <begin position="2"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="259" mass="28644" checksum="E73A06365662B9CB" modified="2009-02-10" version="1">MMKIAFISDVHSNLEALESVLEDIKKENVDKIFCLGDLVGYGPSPNEVIEKIKKENIISVMGNYDDAVGFEKTSCGCSYNSGRETEVGDESLSWTIKVTSKDNKEFLKNLPKKLSLEIEGVKILLVHGSPLNHLLEYVKPSTEPERLKLIAGTVEEDIIVNGHTHLVMAKHLYGKTILNPGSVGRTKDGKSGATYLILEIDKGAFSYKFRFVEYDVKKTIEKIVKVGLPIEHATVLALGSTFNMGPSKNNKNDLGIFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEJ8</accession>
    <name>B7IEJ8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type III restriction enzyme, res subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_4</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579286.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_4"/>
    <dbReference type="EnsemblBacteria" id="ACJ74512">
        <property type="protein sequence ID" value="ACJ74512"/>
        <property type="gene ID" value="THA_4"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_4"/>
    <dbReference type="eggNOG" id="COG1061">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024175_0_0_0"/>
    <dbReference type="OMA" id="GWMIANN"/>
    <dbReference type="OrthoDB" id="377818at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="267"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="531"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="697" mass="81032" checksum="FEEF69EE2FC0AADB" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEK2</accession>
    <name>B7IEK2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Viral A-type inclusion protein repeat, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_8</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the exbB/tolQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579290.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_8"/>
    <dbReference type="EnsemblBacteria" id="ACJ74516">
        <property type="protein sequence ID" value="ACJ74516"/>
        <property type="gene ID" value="THA_8"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_8"/>
    <dbReference type="eggNOG" id="COG5185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_665538_0_0_0"/>
    <dbReference type="OMA" id="AFPAIDQ"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002898">
        <property type="entry name" value="MotA_ExbB_proton_chnl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01618">
        <property type="entry name" value="MotA_ExbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="MotA_ExbB" evidence="8">
        <location>
            <begin position="39"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="413" mass="48438" checksum="024E92955AB46876" modified="2009-02-10" version="1">MFLSFLKGFFTVLLNPFTVIYMLIEFLFFAYYLFKKGSLQNSLNREDNELTEEEIISFTNFWPSFFITLGILGTFIGIIIGIINLDFENENSLKNSINSLLSSMGTAFFSSIIGVIFSIAAQIINKPVVKGLSKITKTNKEKLLDEIIGIRNDIKEFLANPFETISEKANQTVEQYSSSFNQLNETLNNTFEQNINRFSNIVHNISEELNKQFDSIKQQSELIEKREKVIQNEKELLKDFSLIIEKSRETFNEIFEKQKNTVEKLEKVETLIQSIIENKADLNSLNENVLEVINNFKRFTDEFNSNFNEFISSINRVTQQIPNQMSLKYKNAFEEMENFTIEFFSKFKEISVYLYNTIDDFEKSLEYFNYSLNNIRNDIEFAIKDFKNSLIQLLEDYYKISNNINDEEEKKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEK8</accession>
    <name>B7IEK8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integrase, catalytic region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_14</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_14"/>
    <dbReference type="EnsemblBacteria" id="ACJ74522">
        <property type="protein sequence ID" value="ACJ74522"/>
        <property type="gene ID" value="THA_14"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_14"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_31_3_0"/>
    <dbReference type="OMA" id="DEVKCRI"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025948">
        <property type="entry name" value="HTH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13276">
        <property type="entry name" value="HTH_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="100"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="273" mass="32582" checksum="0619AEC24E4C34E0" modified="2009-02-10" version="1">MVKEGFSISEAVRYLKISRSTYYYKPKEYKRKKNNESILKLIQKLKQKHPYWGYRRIYAMLRKQGKKINHKKVYRIMKENGLLLKQKKKKARRVFQSKIIPSRPRQVLGIDMTKVLTKDGGWVNYIAVIDWYTREVLGSQISLRGRTEEWLKALNNALNKGYKDGVRGREVILVSDNGTQPTSRRFMEECKILGIKQIFTSYNNPKGNANTERYFRTYKEEVVWPVEEMSYSELVARTKEYEEFYNSKYPHSALKYRSPREVYEEYLSFTKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEM7</accession>
    <name>B7IEM7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide transport system permease protein AppB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_33</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579307.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_33"/>
    <dbReference type="EnsemblBacteria" id="ACJ74541">
        <property type="protein sequence ID" value="ACJ74541"/>
        <property type="gene ID" value="THA_33"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_33"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_3_0"/>
    <dbReference type="OMA" id="WPGMGTF"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="100"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="345" mass="38779" checksum="58A20DAAE8CECDD2" modified="2009-02-10" version="1">MTAYIIRRLLLLPLILFGVTLIIFSFTEILGPEKMISAYVNPNVFDKLSNEDIDRLIEKYGLNDPMWTRYFKWLGGVLKGELGWSVTAKQPVADAIKERIPYTIELALYAFIPVIAVGIWLGVTAAVNRDKFIDHFIRIFAIVGWSLPDFVFGLIVLMIFYSVLGWFPPGTLSDWADSIVKSDSFHKITHLLTIDALLNGRLDVFWDALRHLIGPILTISWLWWAYLLRITRSSMLEVLGKEYVRTARAKGVPEKEVIHKHAKKNAMIPVVTVAGAMVIGLLAGTVIVEVIFNRVGMGRFTAQAATQLDYAAILGSTLFYSTLLVIGNLIIDILYAILDPRIRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEN4</accession>
    <name>B7IEN4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_40</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579312.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_40"/>
    <dbReference type="EnsemblBacteria" id="ACJ74548">
        <property type="protein sequence ID" value="ACJ74548"/>
        <property type="gene ID" value="THA_40"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_40"/>
    <dbReference type="HOGENOM" id="CLU_1958526_0_0_0"/>
    <dbReference type="OrthoDB" id="2099171at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="128" mass="14828" checksum="3C4A177B6A4BEE85" modified="2009-02-10" version="1">MNNKLKWLVISISLTFTIVSMITVTLFSKGIIQYSWILKVPGRISGGQFIPGMEKLYTKLFLSFLFNTLAILTLSTLFSKFNNKITDEISLVLGMFFLFVSLLSFYNIFQLIAFLFSLFLLYFIFSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEP5</accession>
    <name>B7IEP5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribosomal RNA small subunit methyltransferase C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_51</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579317.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_51"/>
    <dbReference type="EnsemblBacteria" id="ACJ74559">
        <property type="protein sequence ID" value="ACJ74559"/>
        <property type="gene ID" value="THA_51"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_51"/>
    <dbReference type="eggNOG" id="COG2813">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_7_1_0"/>
    <dbReference type="OMA" id="HMIDVNE"/>
    <dbReference type="OrthoDB" id="1816476at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS" evidence="1">
        <location>
            <begin position="29"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="198" mass="22619" checksum="9BCEF286EC29E928" modified="2009-02-10" version="1">MFEHYYTEKPTSVLKVKIVELKLKNGHVYRFKSPSGVYSFGKIDRATKILIENSRINFGDVLDIGCGYGVIGITLKKENPDINLYMSDINQRAVEFAKINSKDNNIEADIRQGYLYEPWNNMSFDHIISNPPIVAGKQVWMNLIEGAFEKLREGGTLELVAYHNKGGKRIKEYMRSIFGNVEELCKEGGIRVYSSKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEW0</accession>
    <name>B7IEW0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103513.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_116"/>
    <dbReference type="EnsemblBacteria" id="ACJ74624">
        <property type="protein sequence ID" value="ACJ74624"/>
        <property type="gene ID" value="THA_116"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_116"/>
    <dbReference type="eggNOG" id="COG1318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109226_0_0_0"/>
    <dbReference type="OMA" id="TRVTWVD"/>
    <dbReference type="OrthoDB" id="1803445at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022285">
        <property type="entry name" value="CHP03879_regulat_dom_put"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03879">
        <property type="entry name" value="near_KaiC_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="144" mass="16605" checksum="1FBC6130BE8F34DA" modified="2009-02-10" version="1">MIPLTPMGKQEIHKLESILLYATLFRKDVLELIKDPSERLTWVDSLAVASSAIAREKAGMRVPEIAEELGRTEQTIRKHLKGESKAGQIVRETYDLLKQGQIDESQISISSILEENEKLKQENYILKNFIKEFYNKVKDYLPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEW8</accession>
    <name>B7IEW8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphate-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_124</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the system for phosphate transport across the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the PstS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579360.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_124"/>
    <dbReference type="EnsemblBacteria" id="ACJ74632">
        <property type="protein sequence ID" value="ACJ74632"/>
        <property type="gene ID" value="THA_124"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_124"/>
    <dbReference type="eggNOG" id="COG0226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026228_5_1_0"/>
    <dbReference type="OMA" id="GTKHGTR"/>
    <dbReference type="OrthoDB" id="1222928at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011862">
        <property type="entry name" value="Phos-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02136">
        <property type="entry name" value="ptsS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PBP_domain" evidence="5">
        <location>
            <begin position="20"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="273" mass="30886" checksum="8B96871C65F13376" modified="2009-02-10" version="1">MRKIFVIFAILLVFFAFSRTLVIKGSNTIFPVAQLWIEELKKMYPDMEITLEGAGSSTGIAALFNETADIANSSRWLKESEIEKMHSMKKYFVPIIVGYDGIAVIVNKNLGIENISIDTLKDIYTGKIRYWSQVDPNLPKKQIVIYSRNTASGTFETFENKVLNKEKMAPWVRMVESTQFEIESVKNNPYAIAYTGIGYVTEDVKVLKVDGVLPTKRNILEGTYPISRPLYMFVDASNGYLVDDLIKKYVNFAVSKKGQDLVEKAGYVSAYGF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEY2</accession>
    <name>APT_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenine phosphoribosyltransferase</fullName>
            <shortName evidence="1">APRT</shortName>
            <ecNumber evidence="1">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">apt</name>
        <name type="ordered locus">THA_138</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103571.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IEY2"/>
    <dbReference type="SMR" id="B7IEY2"/>
    <dbReference type="STRING" id="484019.THA_138"/>
    <dbReference type="EnsemblBacteria" id="ACJ74646">
        <property type="protein sequence ID" value="ACJ74646"/>
        <property type="gene ID" value="THA_138"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_138"/>
    <dbReference type="eggNOG" id="COG0503">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063339_3_0_0"/>
    <dbReference type="OMA" id="KPGIVFR"/>
    <dbReference type="OrthoDB" id="1532478at2"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00646"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00103">
        <property type="entry name" value="PUR_PYR_PR_TRANSFER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0660">Purine salvage</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000116183" description="Adenine phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00004"/>
        </source>
    </evidence>
    <sequence length="170" mass="19085" checksum="FCFD6652F9AE41C3" modified="2009-02-10" version="1">MDLRTFIRDIPDFPEKGIIFRDITPLLKDKEAFKHAIDMLAEKLSEIDFDLIVAPEARGFIFGGALSYKLNKGFIPVRKPGKLPYEVISEKYTLEYGEAELQMHVDSINKGQKVIIFDDVLATGGTAKALKKLVEKAGGEVVAMSFLIELSYLNPRDILKDENIISLIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF32</accession>
    <name>B7IF32_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">OorD subunit of 2-oxoglutarate:acceptor oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_191</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103683.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_191"/>
    <dbReference type="EnsemblBacteria" id="ACJ74696">
        <property type="protein sequence ID" value="ACJ74696"/>
        <property type="gene ID" value="THA_191"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_191"/>
    <dbReference type="eggNOG" id="COG4231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_3_0"/>
    <dbReference type="OMA" id="ACMQCEL"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="75" mass="8453" checksum="0D274CA2ABC6BE53" modified="2009-02-10" version="1">MAKRSFRVNINYDWCKSCGICYHTCPTKTIKEGELKRPVVEDHSTCIGCLICENLCPDFAINIVDITEKVGEKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF37</accession>
    <name>B7IF37_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="10">6-phosphofructokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_196</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579405.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_196"/>
    <dbReference type="EnsemblBacteria" id="ACJ74701">
        <property type="protein sequence ID" value="ACJ74701"/>
        <property type="gene ID" value="THA_196"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_196"/>
    <dbReference type="eggNOG" id="COG0205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020655_0_0_0"/>
    <dbReference type="OMA" id="WRYTNLG"/>
    <dbReference type="OrthoDB" id="1421302at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012003">
        <property type="entry name" value="ATP_PFK_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000532">
        <property type="entry name" value="ATP_PFK_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="SSF53784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PFK" evidence="9">
        <location>
            <begin position="3"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="335" mass="36933" checksum="75356A077A52E8FF" modified="2009-02-10" version="1">MRRVGVLCVGNDSPGINAAIRSAVVKGLDLNIEVMGIKDGFEGLLRDDLEVLLRPNVSNILHQGGTMLGTSLYVPEEDVGKVKEKVEQYGISSLLILGGRLGAKAAINLMKIGVPSVLVPATIDNDLAFTDFSIGFFTAVEHVKNALDVLHSTAESHHRVMIVETMGKPGGWIATIGGLSGGADYIITSTEKPNFEELIEIIKRRYEGKKRFSLIVVESGVELPEEIKKECDGFEKLSSSEIIGKYIEKKLKEKFGIEWRYTNLGYLQRGGSPAAMDRIIATQMAAHAIEMIKMGKVYHAVGVKGFSITEIPYSETMLNYRPVDYYLKELTKLFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF57</accession>
    <name>B7IF57_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_216</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103751.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_216"/>
    <dbReference type="EnsemblBacteria" id="ACJ74721">
        <property type="protein sequence ID" value="ACJ74721"/>
        <property type="gene ID" value="THA_216"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_216"/>
    <dbReference type="eggNOG" id="COG0306">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015355_3_3_0"/>
    <dbReference type="OMA" id="GYDITRT"/>
    <dbReference type="OrthoDB" id="1329125at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PTHR11101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="397" mass="41892" checksum="4E4EB9BF85B9D23B" modified="2009-02-10" version="1">MLLIVGAFLVGFGMAFAIGANDVANSMATAVGAKAITPKQAVLIASILEFLGAILFGAHVTKTIAKGIVDLNMISDPNNILIGAFSALIASTIWILLATFWGMPVSTTHSIVGGMIGFGLAAGGLQIVNWMTLLKIVITWVTSPLIGGAMAYVIFKFISFSILHRKHPAKAAKYVAPILLGVAFYTIAVLFVVKTMKKDILLGNYAGIIIGFIVFLISFLYLRKAKVGDNEYDIVEKIFKKAQVVTSCYVSFSHGANDVANAVGPLALMYIIITTGSVKGAIEIPKYILALGGIGISFGVAILGYRVMKTVGQDITELNNTRGFSIDFSTATTVLIASTMGMPISTTHTVVGAVSGVGFARGIEVVNVGILKNIIISWFVTVPFAAGVSALLYIILT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF61</accession>
    <name>ISPD_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4-diphosphocytidyl-2C-methyl-D-erythritol synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">MEP cytidylyltransferase</fullName>
            <shortName evidence="1">MCT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispD</name>
        <name type="ordered locus">THA_220</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-C-methyl-D-erythritol 4-phosphate + CTP + H(+) = 4-CDP-2-C-methyl-D-erythritol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13429"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:58262"/>
            <dbReference type="EC" id="2.7.7.60"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.60" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579424.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IF61"/>
    <dbReference type="SMR" id="B7IF61"/>
    <dbReference type="STRING" id="484019.THA_220"/>
    <dbReference type="EnsemblBacteria" id="ACJ74725">
        <property type="protein sequence ID" value="ACJ74725"/>
        <property type="gene ID" value="THA_220"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_220"/>
    <dbReference type="eggNOG" id="COG1211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061281_2_3_0"/>
    <dbReference type="OMA" id="ERQHSVY"/>
    <dbReference type="OrthoDB" id="1836139at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00093"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050518">
        <property type="term" value="F:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02516">
        <property type="entry name" value="CDP-ME_synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00108">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001228">
        <property type="entry name" value="IspD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034683">
        <property type="entry name" value="IspD/TarI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018294">
        <property type="entry name" value="ISPD_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01128">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01295">
        <property type="entry name" value="ISPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000191075" description="2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="site" description="Positions MEP for the nucleophilic attack" evidence="1">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="site" description="Positions MEP for the nucleophilic attack" evidence="1">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00108"/>
        </source>
    </evidence>
    <sequence length="226" mass="25587" checksum="424DA710FDE80995" modified="2009-02-10" version="1">MIVGVILFGGKGTRFSKDFPKQFLKFNGKALMEHTVEKFLLDCFEFLVVVSNKDYINKSIEILKKFEKKIYVIEGGKTREHSTFNAIKFLENKIDLEDIVLIHDGARPFVNKDIIEKNIENAKLFGATVTAISSENTIAVVENDFILSVPKRDSIFIIQTPQTFKYKVLKDSFFKFSNRLDNFTDDSSVVLASGYKVSITEGNKKNIKITTVEDLHLMGVDEIVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF68</accession>
    <name>B7IF68_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Basic membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_227</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BMP lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579428.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_227"/>
    <dbReference type="EnsemblBacteria" id="ACJ74732">
        <property type="protein sequence ID" value="ACJ74732"/>
        <property type="gene ID" value="THA_227"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_227"/>
    <dbReference type="eggNOG" id="COG1744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038813_0_0_0"/>
    <dbReference type="OMA" id="LASMMKR"/>
    <dbReference type="OrthoDB" id="603910at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003760">
        <property type="entry name" value="PnrA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02608">
        <property type="entry name" value="Bmp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Bmp" evidence="3">
        <location>
            <begin position="21"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="365" mass="39559" checksum="6DB897E7C4C0EB5D" modified="2009-02-10" version="1">MKKLLIFILSILFVVSAFSFKAIMVTDTGGLGDKSFNDGTWAGIKRAAAELGIDAQVIQSYEQADYVSNLTKAAQEIQKEPDGGIVYAVGFMMTDALFEVAQQFPDVYFAGIDIAPSSGDVPPNVICFLFKEQESAFLVGYIAAATTRANKVGFIGGIPIPPVERFRYGFEVGVKVFNDLHGTNVQIIKGYTNHFSDPKKGKDLALSQFAEGVDIIFHASGACGNGVIEAAKEKMVSLVGKDDLKSILEYAFEKGGYFAMGVDVDQDYMAPGVVLASAMKGVDTASYLGVKWAYEGNWTPGIHKLGLAENGVRMSPMKYTKGLVENRTLAELAYLVTLIKKGILTVPETEEALKSFRTPNIVFPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF99</accession>
    <name>B7IF99_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Riboflavin biosynthesis protein RibD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">Diaminohydroxyphosphoribosylaminopyrimidine deaminase</fullName>
                <shortName evidence="10">DRAP deaminase</shortName>
                <ecNumber evidence="10">3.5.4.26</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">Riboflavin-specific deaminase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="10">5-amino-6-(5-phosphoribosylamino)uracil reductase</fullName>
                <ecNumber evidence="10">1.1.1.193</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">HTP reductase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="15" type="primary">ribD</name>
        <name evidence="15" type="ordered locus">THA_262</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)-pyrimidinedione 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H(+) + H2O = 5-amino-6-(5-phospho-D-ribosylamino)uracil + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21868"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-6-(5-phospho-D-ribitylamino)uracil + NADP(+) = 5-amino-6-(5-phospho-D-ribosylamino)uracil + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17845"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58421"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="EC" id="1.1.1.193"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10 13">Binds 1 zinc ion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">In the C-terminal section; belongs to the HTP reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">In the N-terminal section; belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.26" evidence="10"/>
    <dbReference type="EC" id="1.1.1.193" evidence="10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_262"/>
    <dbReference type="EnsemblBacteria" id="ACJ74763">
        <property type="protein sequence ID" value="ACJ74763"/>
        <property type="gene ID" value="THA_262"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_262"/>
    <dbReference type="eggNOG" id="COG0117">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1985">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036590_1_2_0"/>
    <dbReference type="OMA" id="PYIILKW"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00401"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008835">
        <property type="term" value="F:diaminohydroxyphosphoribosylaminopyrimidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004794">
        <property type="entry name" value="Eubact_RibD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006769">
        <property type="entry name" value="RibD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00326">
        <property type="entry name" value="eubact_ribD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 12" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="8 10 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="14">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ACJ74763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="343" mass="38682" checksum="89DB56BD89089328" modified="2009-02-10" version="1">MEKFMKLALKLAKKGTGRVSPNPLVGAVIVKDGKIISTGYHKRYGDFHAERNAILNAKTSLEGSTLFVNLEPCCHHGKTPPCTDLIIKSKISQVFISILDPNPLVNGKGVDILRKNGIEVHVGLLEKEASYLNRVFIKFITKKTPYVALKVAMTLDGFICDNLGKSKWITKNNFNISHNLRNFFTAIMVGANTVLKDNPQLTCRNGGRNPIRIILDYEGKLAGKNFSVFDDQSRTIVFSSRCEKYNGAECYPETRPEDILERLYSLNIDSVLIEGGASVLSQFYDFSDELHLFYAPKIFGKGLSAFSGIEKYIDEEFDLKIKKIRRLKNEFYLEVIKNVYGNY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFB7</accession>
    <name>SYC_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="1">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cysS</name>
        <name type="ordered locus">THA_281</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579467.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFB7"/>
    <dbReference type="SMR" id="B7IFB7"/>
    <dbReference type="STRING" id="484019.THA_281"/>
    <dbReference type="EnsemblBacteria" id="ACJ74781">
        <property type="protein sequence ID" value="ACJ74781"/>
        <property type="gene ID" value="THA_281"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_281"/>
    <dbReference type="eggNOG" id="COG0215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013528_0_1_0"/>
    <dbReference type="OMA" id="AKYWMHN"/>
    <dbReference type="OrthoDB" id="952207at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="PTHR10890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09190">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00840">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000199088" description="Cysteine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="267"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00041"/>
        </source>
    </evidence>
    <sequence length="469" mass="53815" checksum="580EE67F6AE40D95" modified="2009-02-10" version="1">MAIYITNTETGKKEELKTIEPGVVKMYVCGPTVYNYIHIGNARPAVVFDAFRRFLEYRGYKVIMVQNFTDIDDKIINEANEWGVDFKDVADTFIAEYWKDAQSLGIRAANFHPRTTDYVNEIVDAVEKLISKGYAYTVENGDVYFSVKKFERYGKLSGKKIEDLISGARVEVNTLKKDPLDFALWKAVKPGEPSWDSPWGCGRPGWHIECSVMSQKLLGDTFDIHAGGEDLIFPHHEDEKSQSEALTGKPFARYWMHNGMIITRGDKMSKSIGNVFLVREAVKRYGKDAVKLFLLSKHYRTPIEFSDEIMMENKKAALKVLKTLNRFEEKYPYPKVPKRDEYMNDIEQKFVEALEDDFNTPKAIALIFDLSKELNKAMDEGKDDEALKRYHLITRVFGSILGLFEGGVKISEGENTNKVIEEILKVRQEFRKAKNFEAADKIRDALLNSNVKILDTPDGTKWEILEVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFD3</accession>
    <name>B7IFD3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103900.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_297"/>
    <dbReference type="EnsemblBacteria" id="ACJ74797">
        <property type="protein sequence ID" value="ACJ74797"/>
        <property type="gene ID" value="THA_297"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_297"/>
    <dbReference type="eggNOG" id="COG0432">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096980_1_2_0"/>
    <dbReference type="OMA" id="VSAMHIT"/>
    <dbReference type="OrthoDB" id="1691156at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="PTHR30615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="SSF111038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="136" mass="15766" checksum="AE98FFF62F9A76DB" modified="2009-02-10" version="1">MKSYTEYLWFNTKKRKELIRITDTIEEIVKKSGIKEGLCLVSAMHITAGIIVNDDESGLHKDIWEWLEKLAPVGDYNHHWTGEDNGDAHLKRILTHHQVVLPVTNGKLDLGPWEQIFYAEYDGQRRKRVVVKVIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFD6</accession>
    <name>B7IFD6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NAD/nadp octopine/nopaline dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_300</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579482.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_300"/>
    <dbReference type="EnsemblBacteria" id="ACJ74800">
        <property type="protein sequence ID" value="ACJ74800"/>
        <property type="gene ID" value="THA_300"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_300"/>
    <dbReference type="eggNOG" id="COG0240">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056511_2_0_0"/>
    <dbReference type="OMA" id="LMMLMNA"/>
    <dbReference type="OrthoDB" id="427052at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003421">
        <property type="entry name" value="Opine_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02317">
        <property type="entry name" value="Octopine_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_Gly3P_dh_N" evidence="3">
        <location>
            <begin position="2"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Octopine_DH" evidence="4">
        <location>
            <begin position="182"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="359" mass="39784" checksum="71B3D45CAE1E6C0E" modified="2009-02-10" version="1">MNITVIGAGNGGLALAGFLAMRGFAVTLYNRSLKRISSFIKSRVIKLEGEITGSVKIKNVTNNLEEALKDAELVMIVVPAFAHADIAEKVSKYVKDGQIFILNPGRTFGALEFYNIFKKNRVDKDVIIAETQTFLFASRTSNPGVSHIFRVKNAVPISAIPSTKNEKLKKVIEDIVPEFQVVDSIIYTSFNNIGAVFHPATLILNSGRVESTFGKFEFYLEGITPSVARVLEKIDAERCSVARTLGIEPMTAKDWLNYAYDVLGNNLYEAIHNNAGYQGIIAPPSLQNRYIFEDVPMSLVPISEMAKKLGINTPAIDSIIYLSSIMMGRDFYREGRTLERLGISKLSLDEIKNLIYKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFD7</accession>
    <name>B7IFD7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_301</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103905.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_301"/>
    <dbReference type="EnsemblBacteria" id="ACJ74801">
        <property type="protein sequence ID" value="ACJ74801"/>
        <property type="gene ID" value="THA_301"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_301"/>
    <dbReference type="eggNOG" id="COG0111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_0_0"/>
    <dbReference type="OMA" id="YGSEEVW"/>
    <dbReference type="OrthoDB" id="1435073at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="2">
        <location>
            <begin position="5"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="3">
        <location>
            <begin position="98"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="319" mass="36698" checksum="9F4E4BE0CF107406" modified="2009-02-10" version="1">MKALVLTKITDYFRKKMEEFNEIEWYTPKDIDYVLEDVEIIVTGYLTEEQVDKAKKLKAIFIPWTGADKLPWKKIKERNIIVSNSHGNGKMVAERALSLSLALLGRIIEFHNDLEKGIWHGFAVGFKEDDYWYSLQGKNVAILGTGVIGRHLAKLLKGFDCHITGFKRSKEQIAGFDRIVTSIEEAISDAQVIYLALPLTEKTYKIIDEKMFEKMRGKFLINVGRGELIDEYSLFKALNENILRGFASDVWYKYPSKDEQVILPFNFPIHKFKNVVLSPHVGGFTIEGQQGRIDELFENIESFLKKGFPKTAVDPELMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFE9</accession>
    <name>B7IFE9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Crispr-associated protein, Crm2 family, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_313</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_313"/>
    <dbReference type="EnsemblBacteria" id="ACJ74813">
        <property type="protein sequence ID" value="ACJ74813"/>
        <property type="gene ID" value="THA_313"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_313"/>
    <dbReference type="eggNOG" id="COG1353">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012640_0_0_0"/>
    <dbReference type="OMA" id="WLWDIRQ"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038242">
        <property type="entry name" value="Cmr2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024615">
        <property type="entry name" value="CRISPR-assoc_Cmr2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013407">
        <property type="entry name" value="CRISPR-assoc_prot_Cmr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12469">
        <property type="entry name" value="DUF3692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02577">
        <property type="entry name" value="cas_TM1794_Cmr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="499"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="745"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="827" mass="97294" checksum="3A11C6F96FAB1F09" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFG0</accession>
    <name>B7IFG0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103963.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_324"/>
    <dbReference type="EnsemblBacteria" id="ACJ74824">
        <property type="protein sequence ID" value="ACJ74824"/>
        <property type="gene ID" value="THA_324"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_324"/>
    <dbReference type="HOGENOM" id="CLU_1991609_0_0_0"/>
    <dbReference type="OrthoDB" id="2168087at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="125" mass="14269" checksum="27FB52BDF9377397" modified="2009-02-10" version="1">MRKFFIVLLISIFIIISFGEQNFLTTPSQVESLARVHANEDVNKFWPFLGGFVFGIIAPIYNYVVEPEVPAERLVKLRSIIKSEDDYLIDIYVRTYKDEIKKIKVGMSFAGWGSFIALLAISIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFI7</accession>
    <name>B7IFI7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Stage V sporulation protein S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_357</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104037.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ74851">
        <property type="protein sequence ID" value="ACJ74851"/>
        <property type="gene ID" value="THA_357"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_357"/>
    <dbReference type="eggNOG" id="COG2359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168904_1_0_0"/>
    <dbReference type="OMA" id="IGIEIAC"/>
    <dbReference type="OrthoDB" id="1985350at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007347">
        <property type="entry name" value="SpoVS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35331">
        <property type="entry name" value="PTHR35331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04232">
        <property type="entry name" value="SpoVS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="87" mass="8992" checksum="DA3420E6E9983BD8" modified="2009-02-10" version="1">MEVLKVASGSNPNKVAGALAGVIREKGKAEVQAIGAGAVNQAVKAIAIARGYLAPSGIDLVCVPAFTDVNIENESRTALKFIVFPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFM9</accession>
    <name>B7IFM9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_400</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_400"/>
    <dbReference type="EnsemblBacteria" id="ACJ74893">
        <property type="protein sequence ID" value="ACJ74893"/>
        <property type="gene ID" value="THA_400"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_400"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_622448_0_0_0"/>
    <dbReference type="OMA" id="VWILNTH"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="236"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="440" mass="50799" checksum="319095A79875A36C" modified="2009-02-10" version="1">MIGWTIPPYIGVPVWRTFFEKYTDIYINPITSFISATLISSWFLIPFTSFFLYTFSETQNKKYLESFLLESNKTDIYYKKIVVPNIKGAIYSAFILNFLKAFKDFQVPWLLAEGGAPLSFGITDKGIIGATTNLEVLIYKLFNFESDISQVSSISLLTILIITSVYFLSSKLNSKIKMNISYFSKTLSYIWIFSIIYFFYILLTLAFSNSQSIYLKGSFTLKNFKYILDDGIVKSFSNTLLISIITSILSIILSMYFAYFLLNIKNGENILNFFNFLKIFSGLHIIIFIFYIYSKLRLLNTFTSVILMLVAKNLPFLSLLSYHYFKNFPEDLFLLAKLDNIPKNTFFFKILLPNSFPLVSSLFLLSTLNSFNAFLPPLVFLFDESKYTLSIKLYSYVGAANTHYPLWNLFGAASFATFLILLILTFLLLKISKISYIKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFT7</accession>
    <name>B7IFT7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104238.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_460"/>
    <dbReference type="EnsemblBacteria" id="ACJ74951">
        <property type="protein sequence ID" value="ACJ74951"/>
        <property type="gene ID" value="THA_460"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_460"/>
    <dbReference type="HOGENOM" id="CLU_1854282_0_0_0"/>
    <dbReference type="OMA" id="QAIPVEF"/>
    <dbReference type="OrthoDB" id="1995305at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857389" evidence="1">
        <location>
            <begin position="19"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="148" mass="16769" checksum="05B7BFFDE3759DF5" modified="2009-02-10" version="1">MKKLYFFLLVILASLAFSFDFEPYAGWNVTIDSSNTSSFYSVFLDFPLTVNNDTEFGISLGYIGTNNFVLQSFGKYNIKTSFGIFTTYGKGGAVFSSDFKLNSIGYSVLAGVRYYFKNFFVGFSYAVYYIENEKIGTIPLEIGYKIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFW9</accession>
    <name>B7IFW9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase E</fullName>
            <ecNumber evidence="6">2.1.1.193</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_492</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) + N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42920"/>
            <dbReference type="Rhea" id="RHEA-COMP:10283"/>
            <dbReference type="Rhea" id="RHEA-COMP:10284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74502"/>
            <dbReference type="EC" id="2.1.1.193"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the RNA methyltransferase RsmE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.193" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104297.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_492"/>
    <dbReference type="EnsemblBacteria" id="ACJ74983">
        <property type="protein sequence ID" value="ACJ74983"/>
        <property type="gene ID" value="THA_492"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_492"/>
    <dbReference type="eggNOG" id="COG1385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067442_4_1_0"/>
    <dbReference type="OMA" id="LIFDEHE"/>
    <dbReference type="OrthoDB" id="1490892at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006700">
        <property type="entry name" value="rRNA_ssu_MeTrfase-E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30027">
        <property type="entry name" value="PTHR30027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04452">
        <property type="entry name" value="Methyltrans_RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015601">
        <property type="entry name" value="MTase_slr0722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00046">
        <property type="entry name" value="TIGR00046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="226" mass="26260" checksum="F1C1EB5F9814A6AB" modified="2009-02-10" version="1">MPNLFFGIKEDNNLIFDEHETVHIKTVRKKENDIIECTDGKGYYYKVKITQIGKKKSFGEILESKYIENNEKTFLSLFAPASRWERLRWLIEKSVELGVDNIYITKTAFSNRDYKDKVEKINMVIRDSAKQCVRFHFPNVDFITFKNIKNYATKNTYFLDFNGQKLPANIDGDVSIIVGPEGGFSKEELDFLFSNFNAIRLGNKILRFETAAFVALSYFAIVLNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFY6</accession>
    <name>B7IFY6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="7">4.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pyrF</name>
        <name evidence="9" type="ordered locus">THA_511</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H(+) + orotidine 5'-phosphate = CO2 + UMP</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the OMP decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100667.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_511"/>
    <dbReference type="EnsemblBacteria" id="ACJ75000">
        <property type="protein sequence ID" value="ACJ75000"/>
        <property type="gene ID" value="THA_511"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_511"/>
    <dbReference type="eggNOG" id="COG0284">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067069_0_0_0"/>
    <dbReference type="OMA" id="YVKVGHN"/>
    <dbReference type="OrthoDB" id="1150446at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119:SF2">
        <property type="entry name" value="PTHR32119:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OMPdecase" evidence="8">
        <location>
            <begin position="2"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="203" mass="23046" checksum="A8A97FAD3C709E23" modified="2009-02-10" version="1">MIPVLSLDMEKPLEFIDKYGSFDTVKVGHNLAIFGKKILDEFEKRNIKVILDLKFCDIPSTVSRSIKSWDHPAIIGFTVHSAAGIESVKAALDSTEKIIFSVVKLTSQVGELSDYLKKIEELENIKSSFVLPGRWAINLRKKLSGKFLVPGIRMQVKADDQKDIITLDQIKDIADFAVLGREIYLSKNPKEKIEKIKEELKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFY7</accession>
    <name>B7IFY7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="4">OPRT</shortName>
            <shortName evidence="4">OPRTase</shortName>
            <ecNumber evidence="2 4">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">pyrE</name>
        <name evidence="6" type="ordered locus">THA_512</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + orotate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="2 4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579625.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_512"/>
    <dbReference type="EnsemblBacteria" id="ACJ75001">
        <property type="protein sequence ID" value="ACJ75001"/>
        <property type="gene ID" value="THA_512"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_512"/>
    <dbReference type="eggNOG" id="COG0461">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074878_3_0_0"/>
    <dbReference type="OMA" id="YFQCAKV"/>
    <dbReference type="OrthoDB" id="1280396at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006273">
        <property type="entry name" value="Orotate_PRibTrfase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01367">
        <property type="entry name" value="pyrE_Therm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="5">
        <location>
            <begin position="35"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-phosphoribose 1-diphosphate binding" evidence="4">
        <location>
            <begin position="111"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="188" mass="21128" checksum="A8A194D5D3B054DE" modified="2009-02-10" version="1">MEIKEILEKTGALLTGHFLLSSGNHSEKYVQCARLFEFPEYGDMVAKMLAEKIKKYKPDLIIGPAMGGIHLAYSVSKYLNIRNIFAERENGLMTLRRGFKINKGERVAIVEDVITTGKSVKEVIDIVNESGGNLCCIGSIINRSNSNPFDVPYEYLIKLELPIYSPDECPLCKKNIPLEKPGSRFIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFZ5</accession>
    <name>B7IFZ5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pyruvate synthase subunit PorD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_520</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100655.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_520"/>
    <dbReference type="EnsemblBacteria" id="ACJ75009">
        <property type="protein sequence ID" value="ACJ75009"/>
        <property type="gene ID" value="THA_520"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_520"/>
    <dbReference type="eggNOG" id="COG1144">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_1_1_0"/>
    <dbReference type="OMA" id="GSWRTFK"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011898">
        <property type="entry name" value="PorD_KorD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724">
        <property type="entry name" value="PTHR43724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02179">
        <property type="entry name" value="PorD_KorD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="33"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="110" mass="12557" checksum="D5D2DA775667245E" modified="2009-02-10" version="1">MAELKGWKDIPIGGVIDKPATAKEYQTGTWRVIRPVHQPENCIHCMMCWLYCPDQAIVIEVVDGKPKMKGYNYYYCKGCGLCANVCPKTNDNLPEEKRAIVMKPETEFQD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG29</accession>
    <name>FTHS_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Formate--tetrahydrofolate ligase</fullName>
            <ecNumber evidence="1">6.3.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formyltetrahydrofolate synthetase</fullName>
            <shortName evidence="1">FHS</shortName>
            <shortName evidence="1">FTHFS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fhs</name>
        <name type="ordered locus">THA_555</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-formyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20221"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the formate--tetrahydrofolate ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.3" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579645.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IG29"/>
    <dbReference type="SMR" id="B7IG29"/>
    <dbReference type="STRING" id="484019.THA_555"/>
    <dbReference type="PRIDE" id="B7IG29"/>
    <dbReference type="EnsemblBacteria" id="ACJ75043">
        <property type="protein sequence ID" value="ACJ75043"/>
        <property type="gene ID" value="THA_555"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_555"/>
    <dbReference type="eggNOG" id="COG2759">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003601_3_3_0"/>
    <dbReference type="OMA" id="CGEIMTM"/>
    <dbReference type="OrthoDB" id="177859at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00477">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000146705" description="Formate--tetrahydrofolate ligase">
        <location>
            <begin position="1"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="65"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01543"/>
        </source>
    </evidence>
    <sequence length="551" mass="59873" checksum="861332D608F65B9E" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG60</accession>
    <name>B7IG60_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VanZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_587</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579662.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_587"/>
    <dbReference type="EnsemblBacteria" id="ACJ75074">
        <property type="protein sequence ID" value="ACJ75074"/>
        <property type="gene ID" value="THA_587"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_587"/>
    <dbReference type="eggNOG" id="COG5652">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096028_0_3_0"/>
    <dbReference type="OMA" id="EMLIRKT"/>
    <dbReference type="OrthoDB" id="2074871at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006976">
        <property type="entry name" value="VanZ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04892">
        <property type="entry name" value="VanZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="VanZ" evidence="2">
        <location>
            <begin position="8"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="177" mass="20844" checksum="1EB96BF56F08F05A" modified="2009-02-10" version="1">MKKAIFIFILIILWILLIFYFSSRNPLYSSQQSSFVTKILRKIDQIIDFSDTTLFKKVEIFLKKLWFKTQYVPAEMLVRKTAHFGLYFILGFFSFFGFHLFIKNIFSILLAITFPNLIAVLDEYTQQYYNRGSSLNDVIIDLSGITFGVAFAVIVYYTFKLIRKIFKKKGNSENLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG67</accession>
    <name>B7IG67_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">O-Antigen Polymerase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_594</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579668.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_594"/>
    <dbReference type="EnsemblBacteria" id="ACJ75081">
        <property type="protein sequence ID" value="ACJ75081"/>
        <property type="gene ID" value="THA_594"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_594"/>
    <dbReference type="eggNOG" id="COG3307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011262_0_0_0"/>
    <dbReference type="OMA" id="GWNNFKR"/>
    <dbReference type="OrthoDB" id="386044at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007016">
        <property type="entry name" value="O-antigen_ligase-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04932">
        <property type="entry name" value="Wzy_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="226"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="1079" mass="125791" checksum="2A825C5435528093" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG85</accession>
    <name>B7IG85_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Regulator of cell autolysis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_613</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579682.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_613"/>
    <dbReference type="EnsemblBacteria" id="ACJ75099">
        <property type="protein sequence ID" value="ACJ75099"/>
        <property type="gene ID" value="THA_613"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_613"/>
    <dbReference type="eggNOG" id="COG3275">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020473_3_3_0"/>
    <dbReference type="OMA" id="SHFFRSN"/>
    <dbReference type="OrthoDB" id="1031920at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010559">
        <property type="entry name" value="Sig_transdc_His_kin_internal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011620">
        <property type="entry name" value="Sig_transdc_His_kinase_LytS_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07694">
        <property type="entry name" value="5TM-5TMR_LYT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06580">
        <property type="entry name" value="His_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="5TM-5TMR_LYT" evidence="8">
        <location>
            <begin position="28"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="His_kinase" evidence="7">
        <location>
            <begin position="363"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="554" mass="61905" checksum="59BEECC0A4D84B95" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGA2</accession>
    <name>B7IGA2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein translocase subunit SecE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">secE</name>
        <name evidence="8" type="ordered locus">THA_633</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579694.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_633"/>
    <dbReference type="EnsemblBacteria" id="ACJ75116">
        <property type="protein sequence ID" value="ACJ75116"/>
        <property type="gene ID" value="THA_633"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_633"/>
    <dbReference type="eggNOG" id="COG0690">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113663_8_0_0"/>
    <dbReference type="OMA" id="SKFWIFL"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005807">
        <property type="entry name" value="SecE_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038379">
        <property type="entry name" value="SecE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33910">
        <property type="entry name" value="PTHR33910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00964">
        <property type="entry name" value="secE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01067">
        <property type="entry name" value="SECE_SEC61G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="62" mass="7224" checksum="452A8F9B4E3D5BE8" modified="2009-02-10" version="1">MEKLKKFFREVKTEIKKTHWPNRKELWGATGVVLVILLVTGVYFFALDLIFSGALSALFKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGC0</accession>
    <name>B7IGC0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_657</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75134">
        <property type="protein sequence ID" value="ACJ75134"/>
        <property type="gene ID" value="THA_657"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_657"/>
    <dbReference type="HOGENOM" id="CLU_3277927_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="41" mass="4850" checksum="D5CC3B81D07BFE9C" modified="2009-02-10" version="1">MDKVMLLGNHVVLSKEITELEELLQDIFEEEELEEVFEITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGD2</accession>
    <name>B7IGD2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribose transport system permease protein RbsC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_671</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579715.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_671"/>
    <dbReference type="EnsemblBacteria" id="ACJ75146">
        <property type="protein sequence ID" value="ACJ75146"/>
        <property type="gene ID" value="THA_671"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_671"/>
    <dbReference type="eggNOG" id="COG1172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028880_2_2_0"/>
    <dbReference type="OMA" id="FDVWNKR"/>
    <dbReference type="OrthoDB" id="1254678at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="308" mass="32671" checksum="D28D095C4AFA4CCB" modified="2009-02-10" version="1">MNWKAVLKKAKEYPAIFGFISLVIFFALLSDRFLSLSNFINVFRQVSIQAIIAFGMTLVIISGGIDLSVGSVFALSAVIMASVIKGYSVFLGILVALVVGAIFGSINGFIIAKGKIQPFIVTLATMAIARSLTLVYTQGMPITGFPASFRFIGRGEIFGIPVPIVIMFSIFAIILYISIYTKLGLYTYAIGGNETAAKLSGVKVDKYKIIIYMLSGLLSAIAAIILTSRLNSAQPTFGTGYELDAIAAVVLGGASLAGGKGTVIGTLFGALIMGILNNGLNLLNISPFYQQAVKGIVILIAVLIEREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGI8</accession>
    <name>B7IGI8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="4">ATase</shortName>
            <ecNumber evidence="4">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="4">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 10" type="primary">purF</name>
        <name evidence="10" type="ordered locus">THA_736</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = 5-phospho-alpha-D-ribose 1-diphosphate + H2O + L-glutamine</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="4 7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="4 8">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 4 5">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4 5">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579762.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_736"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="EnsemblBacteria" id="ACJ75202">
        <property type="protein sequence ID" value="ACJ75202"/>
        <property type="gene ID" value="THA_736"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_736"/>
    <dbReference type="eggNOG" id="COG0034">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_3_3_0"/>
    <dbReference type="OMA" id="ENAQPTF"/>
    <dbReference type="OrthoDB" id="267682at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 5 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 8" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4 5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="9">
        <location>
            <begin position="2"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4 6">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="431" mass="47818" checksum="E5A6566BDEF3F62F" modified="2009-02-10" version="1">MCGIAGVWNVEKSYNVLHDLLLGLQHRGQESVGIVLEDFKMIKNKGLVSQVLSESNFLPGNSGIGHVRYSTYGSNTEIQPLIGITSKGKFAIAHNGNIPDAESRINSLVEKGSVFNSTLDTEIFLHYISLAPYSDPILSIQWAFSRISSAYSVVILGKDKLIAARDRFGFRPLFYGKYKNGYVIASEDSVLYSIGANKIVEVLPGEIVIFSDSGIEKVKYANTDVKFCSFEYIYFSRPDSNFLYGNVHRARYRMGEILFEESSMRGDIVIPILDSGFSGALGFSAASKIPIELGLMRNRYLGRSFIMPENREEIIKRKLVPIPEVIKDKEVIIIDDSIVRGTTMKQIVKMLKENGARKVKVGIHSPPVIGPCPYGIDTSRKNELIANQKSIDEILSEINADELHYLSVEGLKKAIGGDNLCLGCLNLNYPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGP2</accession>
    <name>B7IGP2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CheY-P phosphatase CheX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_794</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579800.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_794"/>
    <dbReference type="EnsemblBacteria" id="ACJ75256">
        <property type="protein sequence ID" value="ACJ75256"/>
        <property type="gene ID" value="THA_794"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_794"/>
    <dbReference type="eggNOG" id="COG1406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116290_1_1_0"/>
    <dbReference type="OMA" id="NVEYINP"/>
    <dbReference type="OrthoDB" id="1956590at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038756">
        <property type="entry name" value="CheX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028051">
        <property type="entry name" value="CheX-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39452">
        <property type="entry name" value="PTHR39452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13690">
        <property type="entry name" value="CheX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="SSF103039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheX" evidence="2">
        <location>
            <begin position="42"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="155" mass="16831" checksum="B7794B1217F0D724" modified="2009-02-10" version="1">MDAKIINSLITAVNSTFNMVLQVEPKVLKPEIAKGIDPKYPIVTVIGFNGDIEGNLIYSFNEETAKNVVSKMMGMPYDNLDELALSALGELGNMASGSIAMNLEKNNYKVDITPPTVITGKEIQITAEGVILKLPLNIFSEGDFEIHMVIRSGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGS7</accession>
    <name>B7IGS7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_829</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100441.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_829"/>
    <dbReference type="EnsemblBacteria" id="ACJ75291">
        <property type="protein sequence ID" value="ACJ75291"/>
        <property type="gene ID" value="THA_829"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_829"/>
    <dbReference type="HOGENOM" id="CLU_1618217_0_0_0"/>
    <dbReference type="OMA" id="NNIDAEY"/>
    <dbReference type="OrthoDB" id="2122052at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="167" mass="18286" checksum="29B8E2416D6C3D7D" modified="2009-02-10" version="1">MKKLLVSLFVLFVISAFSFPFFAGLEGINVPPTTLSATPSLLTVNARLDLGMFYFSLPVAELYEDGGYNFNNDFSTDFILSHLNVGISLKPKIPMIPLYARVGADLPIFDLLVNQTFSTVDLKVGAGFKFLLLALEGGVVARFEKQNDGSMNMDFGNIFYFAAGVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGT2</accession>
    <name>B7IGT2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulatory protein ResD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_834</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100448.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_834"/>
    <dbReference type="EnsemblBacteria" id="ACJ75296">
        <property type="protein sequence ID" value="ACJ75296"/>
        <property type="gene ID" value="THA_834"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_834"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_8_0"/>
    <dbReference type="OMA" id="FSCYKDD"/>
    <dbReference type="OrthoDB" id="1630092at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="5"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="119" mass="13544" checksum="E5F187E26A9A0B61" modified="2009-02-10" version="1">MSKKKILVVEDEDNMRLLITEELEESGYDVDEARNGEEALKKFREKEYDLVTVDIEMPGMNGLEVAGKIREIKKDAKIIILTAYSHYKSDLASWAADAYIVKSSDLTELKDTIAKIINM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGU6</accession>
    <name>B7IGU6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_849</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579830.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_849"/>
    <dbReference type="EnsemblBacteria" id="ACJ75310">
        <property type="protein sequence ID" value="ACJ75310"/>
        <property type="gene ID" value="THA_849"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_849"/>
    <dbReference type="eggNOG" id="ENOG5033REG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_707409_0_0_0"/>
    <dbReference type="OMA" id="AKIGNGW"/>
    <dbReference type="OrthoDB" id="800882at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005534">
        <property type="entry name" value="Curli_assmbl/transp-comp_CsgG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03783">
        <property type="entry name" value="CsgG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="396" mass="43654" checksum="ECBB7D54329DA63B" modified="2009-02-10" version="1">MKKLAIFLIFVSLLSSLFAITVSEKIGIVILPAKIGNGWNMDEADFLISILEQKALELGRFRVFSRNDLDMIVKERNLGDLGIVEQTFEAGKILGARYAILLTLTELTSNYEKNGYTASLRLSLKLYDLKTGELLASTPFAKETYTEEETPQKAINSILMSTAEDMWLSLREYFKLEAYVSKIEGNKIYLAGLDPKIAKNGFIFKLETSTGEIGYAEVIGIDRNNNLVIAKFKYGAMPSVYDPAIEYPVSSMFGGISAGLYAGKIALGFVGWQNNLYVESGIVLSSFAGYIPGYVTLGGNFDLIEVGQLTETIYGGLQILAIYDTETTSENSFLPIFGINLGTRIKYEFEPKSGIFVSVGASALFPDSFSQSIYDILFGMDTTSFLQIGAGYFMSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGV8</accession>
    <name>B7IGV8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nuclease-related domain family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579842.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_862"/>
    <dbReference type="EnsemblBacteria" id="ACJ75322">
        <property type="protein sequence ID" value="ACJ75322"/>
        <property type="gene ID" value="THA_862"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_862"/>
    <dbReference type="eggNOG" id="ENOG50335QB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078999_0_0_0"/>
    <dbReference type="OMA" id="CHALMIP"/>
    <dbReference type="OrthoDB" id="802050at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011528">
        <property type="entry name" value="NERD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08378">
        <property type="entry name" value="NERD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50965">
        <property type="entry name" value="NERD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NERD" evidence="1">
        <location>
            <begin position="62"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="233" mass="27728" checksum="94A9D6BC998B4927" modified="2009-02-10" version="1">MFARILRQNSEIKEKFYNLISEKENNQKRIFLEKFDKNLFLKVLKPFANIWYTYELKSKKKKGDDGETDVSFFLWICLSRKAIIVNDVVLEVAKGEFIQIDHLIFHQKGIFVIETKNWNGALICDKDIWRLKVGNTWQEVSSPSKQNQRHVRLLLKWFENNLPERLYEKIKDYVFPVVVFPRSNWIKVKNCSMPVFKGKLSLLLYIISQKNKIFEEHELTYVAAKMVSAKPFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGW3</accession>
    <name>B7IGW3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">dTDP-glucose 4,6-dehydratase</fullName>
            <ecNumber evidence="4 5">4.2.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rfbB</name>
        <name evidence="7" type="ordered locus">THA_867</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O</text>
            <dbReference type="Rhea" id="RHEA:17221"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57477"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="EC" id="4.2.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.46" evidence="4 5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104368.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_867"/>
    <dbReference type="EnsemblBacteria" id="ACJ75327">
        <property type="protein sequence ID" value="ACJ75327"/>
        <property type="gene ID" value="THA_867"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_867"/>
    <dbReference type="eggNOG" id="COG1088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_14_0"/>
    <dbReference type="OMA" id="KLIPLMC"/>
    <dbReference type="OrthoDB" id="707977at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01181">
        <property type="entry name" value="dTDP_gluc_dehyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="6">
        <location>
            <begin position="4"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="356" mass="41570" checksum="3969C84805EF81D4" modified="2009-02-10" version="1">MKLLVTGCCGFIGSNFVYYYLEKHKEAQIIGLDKLTYAGNLDNLSKLSDDQKKRFEFVRGDINNGELIEYLIEKYDIDVIVNFAAESHVDRSIYNPREFLETNVLGVQTLLDVVRKKWNKNGKWIEGKKFIQISTDEVYGTLGPSGKFTEKTPISPRSPYSASKASADLLVLAYHETYGMPVNITRCSNNYGPYQFPEKLIPLMILNALEHKNLPIYGDGRQIRDWIHVLDHCRAIDLVIEKGKVGEVYNVGADNEWENIKIVRKIIEILREFTKDEEINENLIKHVKDRPGHDRRYAIDSTKIQHELGWKPEITFEKGLRDTIKWYLDNKEWIEKIRSGEYRDWYEKHYVSEKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH06</accession>
    <name>B7IH06_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA gyrase subunit B</fullName>
            <ecNumber evidence="10">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">gyrB</name>
        <name evidence="12" type="ordered locus">THA_911</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type II topoisomerase GyrB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the type II topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100787.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_911"/>
    <dbReference type="EnsemblBacteria" id="ACJ75370">
        <property type="protein sequence ID" value="ACJ75370"/>
        <property type="gene ID" value="THA_911"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_911"/>
    <dbReference type="eggNOG" id="COG0187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006146_1_2_0"/>
    <dbReference type="OMA" id="LWETTMH"/>
    <dbReference type="OrthoDB" id="205481at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03366">
        <property type="entry name" value="TOPRIM_TopoIIA_GyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01898">
        <property type="entry name" value="GyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002288">
        <property type="entry name" value="DNA_gyrase_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011557">
        <property type="entry name" value="GyrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034160">
        <property type="entry name" value="TOPRIM_GyrB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00986">
        <property type="entry name" value="DNA_gyraseB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01059">
        <property type="entry name" value="gyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="11">
        <location>
            <begin position="412"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ75370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="627" mass="71005" checksum="84C373C41004B73D" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH18</accession>
    <name>B7IH18_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579881.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_923"/>
    <dbReference type="EnsemblBacteria" id="ACJ75382">
        <property type="protein sequence ID" value="ACJ75382"/>
        <property type="gene ID" value="THA_923"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_923"/>
    <dbReference type="HOGENOM" id="CLU_655414_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002854531" evidence="1">
        <location>
            <begin position="19"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="419" mass="47781" checksum="0CFCB25DE5501794" modified="2009-02-10" version="1">MKKFLSFLILLSISISLAQDLPIIKLDTLEKNSFSLIAEENLDAVFIPKIDNLFLFLSSKVLNYNDISLIQFGVGYEGLIINPYLLVSFGQSGKNNQTYESSVSSTSTDYSYDSQASFGILFGGEYDILSNKMSFIVSYDNITQTFTSNDVIYKISTYYTSDENGKQTGWKVSSGKNTISLVSEIPFSIYNINFSGLIAYIYTNKNEFASKIEDDEEIYNFNYNKRGNYFGITPKINFEAENIYFKIGIPFEYENSNYSAKITNTSTMVNSKYPEFLVEESFESTTNLYLYFSIDSSLTNKISLNAELLMNVISYNIKEENNYQYKDLTNIDLENFNKNSTDTTVTKTQNVNAKNYYKPSIYSTFTLTFNLTQNLTLSTTIDQSFLSDILLGKVIPYIISGNTWKVNAPFIKTTLKYNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH67</accession>
    <name>B7IH67_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide deformylase</fullName>
            <shortName evidence="2">PDF</shortName>
            <ecNumber evidence="2">3.5.1.88</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Polypeptide deformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">def</name>
        <name evidence="3" type="ordered locus">THA_972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100939.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_972"/>
    <dbReference type="EnsemblBacteria" id="ACJ75431">
        <property type="protein sequence ID" value="ACJ75431"/>
        <property type="gene ID" value="THA_972"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_972"/>
    <dbReference type="eggNOG" id="COG0242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061901_4_2_0"/>
    <dbReference type="OMA" id="VCIQHEI"/>
    <dbReference type="OrthoDB" id="1649129at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PTHR10458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004749">
        <property type="entry name" value="Pep_def"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00079">
        <property type="entry name" value="pept_deformyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="165" mass="19300" checksum="EDED6DA310CC071E" modified="2009-02-10" version="1">MKIRLYGDPILRKKAKIVEDFEYIQQIKEDLLKIMYLEDGVGLAAPQVGISLRFFAMDDGSGPLIIVNPEIIEHSQEKEIGEEGCLSLPGIFEDVERYKWVKLRYQDEYGKVQEKLFEGYSARIVQHERDHLDGILFIDHLPTSVKRRLSTELSKIMRMRMEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHA0</accession>
    <name>B7IHA0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoesterase</fullName>
            <ecNumber evidence="2">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1006</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579925.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1006"/>
    <dbReference type="EnsemblBacteria" id="ACJ75464">
        <property type="protein sequence ID" value="ACJ75464"/>
        <property type="gene ID" value="THA_1006"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1006"/>
    <dbReference type="eggNOG" id="COG0622">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063749_3_2_0"/>
    <dbReference type="OMA" id="ADTHVPK"/>
    <dbReference type="OrthoDB" id="1355703at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="PTHR11124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="156" mass="17851" checksum="F200C91F4EC7D685" modified="2009-02-10" version="1">MRFLVISDLHIPTRNFQVHPKIVEIAKECDGIFALGDFVDLNTVLLLQSLNRNFHGVFGNMDDYDVKDYLPAQKVVKIGKFTIGLTHGSGSHIRIPERIVNWFDNDVNVILYGHSHVPDDRVYRGKRFINPGTAMETYGIMEINGDSLKFEVFKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHE9</accession>
    <name>B7IHE9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1057"/>
    <dbReference type="EnsemblBacteria" id="ACJ75513">
        <property type="protein sequence ID" value="ACJ75513"/>
        <property type="gene ID" value="THA_1057"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1057"/>
    <dbReference type="HOGENOM" id="CLU_3318378_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="39" mass="4958" checksum="3DF236F443DDAE95" modified="2009-02-10" version="1">MNMKENKRIFFLISLERIFWTKIRLYRYGGLFYIIRLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHF9</accession>
    <name>B7IHF9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobalt-precorrin-8X methylmutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1067</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CobH/CbiC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579974.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1067"/>
    <dbReference type="EnsemblBacteria" id="ACJ75523">
        <property type="protein sequence ID" value="ACJ75523"/>
        <property type="gene ID" value="THA_1067"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1067"/>
    <dbReference type="eggNOG" id="COG2082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084703_1_1_0"/>
    <dbReference type="OMA" id="GAPIFCD"/>
    <dbReference type="OrthoDB" id="1812068at2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016993">
        <property type="term" value="F:precorrin-8X methylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003722">
        <property type="entry name" value="Cbl_synth_CobH/CbiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036588">
        <property type="entry name" value="CobH/CbiC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02570">
        <property type="entry name" value="CbiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63965">
        <property type="entry name" value="SSF63965"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiC" evidence="5">
        <location>
            <begin position="8"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="205" mass="23010" checksum="B754C8CDC90578FF" modified="2009-02-10" version="1">MKYTDPFEIEKKSFLIIRKNLKKEHKPGYFDIVTRVIHATADFEFEELLRFKNDPISVFKETVKNGANIVVDTKMIKAGISNKFLEKYNVKVKCYIDDEDVINQAKISNCTRSILAMKKAIEEKNNDIFVIGNAPTALFTLCSYIENSSFLPKLVIGVPVGFVGAQESKEVLNLLDVPSITTKGRKGGSTVAVAIVNALFRLLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHG1</accession>
    <name>B7IHG1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Precorrin-6y C5,15-methyltransferase (Decarboxylating), CbiE subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cbiE</name>
        <name evidence="6" type="ordered locus">THA_1069</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101140.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1069"/>
    <dbReference type="EnsemblBacteria" id="ACJ75525">
        <property type="protein sequence ID" value="ACJ75525"/>
        <property type="gene ID" value="THA_1069"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1069"/>
    <dbReference type="eggNOG" id="COG2241">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089162_2_0_0"/>
    <dbReference type="OMA" id="DPCFSGL"/>
    <dbReference type="OrthoDB" id="1195697at2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11644">
        <property type="entry name" value="Precorrin-6Y-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012818">
        <property type="entry name" value="CbiE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02467">
        <property type="entry name" value="CbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="199" mass="22326" checksum="821C2DC4D1A43BFE" modified="2009-02-10" version="1">MITVVGVGPGNPKLLTSYALKKIKEAKVLVGGQRLLEQFDTRAKKIPYDRNFDLKSIDKLGDGVVILASGDPLLYGIANTLLKNFGKEKIEIIPGISCVQYMASKLKVSLNDAKIVSFHGRKGKLEEILNYKKIFIFTDKVNTPQKIAKDLRKASCTDYVIFVGENLSYENEKICRFTLKELAEYEYEFGINLVLLLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHG5</accession>
    <name>B7IHG5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CbiG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1073</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579979.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1073"/>
    <dbReference type="EnsemblBacteria" id="ACJ75529">
        <property type="protein sequence ID" value="ACJ75529"/>
        <property type="gene ID" value="THA_1073"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1073"/>
    <dbReference type="eggNOG" id="COG2073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028397_0_0_0"/>
    <dbReference type="OMA" id="GIGCNRG"/>
    <dbReference type="OrthoDB" id="1617773at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021745">
        <property type="entry name" value="CbiG_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021744">
        <property type="entry name" value="CbiG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002750">
        <property type="entry name" value="CobE/GbiG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036518">
        <property type="entry name" value="CobE/GbiG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038029">
        <property type="entry name" value="GbiG_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01890">
        <property type="entry name" value="CbiG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11761">
        <property type="entry name" value="CbiG_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11760">
        <property type="entry name" value="CbiG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159664">
        <property type="entry name" value="SSF159664"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159672">
        <property type="entry name" value="SSF159672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiG_N" evidence="2">
        <location>
            <begin position="48"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="CbiG_mid" evidence="3">
        <location>
            <begin position="134"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="CbiG_C" evidence="1">
        <location>
            <begin position="210"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="330" mass="36985" checksum="F18C1F59A1B7B22C" modified="2009-02-10" version="1">MKIAILSITKNGAELSKRLFNSIKCDVYVKEDFKFNGAKVIDFLLKRFVKKIFKMYDALVFIMATGIVVRVIADCLKDKFDDPAVVVIDEKGNFVISLLSGHVGGANELAKRLSKILDAVPVITTASDVNGIESIDMIAKRYGFEIERREDLSKVSAAIVNKEHVDFILDSELDIDVESKSYIDSTAQVYITNKVVFSKKPHVILRPKNLVVGIGCKKNIQIKKLLQFVESSLVFLNLSKNSIRAIATIELKKKEDALLKLSKYLNVPLKIYSVEDIKKVESLFEGSEFVKKIIGVGSVARPVGYLESNMGKELFYSKKDGMVLSIYQDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHI0</accession>
    <name>B7IHI0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNase H</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rnhA</name>
        <name evidence="2" type="ordered locus">THA_1088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1088"/>
    <dbReference type="EnsemblBacteria" id="ACJ75544">
        <property type="protein sequence ID" value="ACJ75544"/>
        <property type="gene ID" value="THA_1088"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1088"/>
    <dbReference type="eggNOG" id="COG0328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030894_2_2_0"/>
    <dbReference type="OMA" id="ITLYYDY"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="29"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="177" mass="20499" checksum="6BEB4659C3D29548" modified="2009-02-10" version="1">MFFNKKREATAIKILAATSKEVANEFAENILCIKVYIDGSYSSETKKIGFSIVILSQKIEKFYKSIENEELAKHRNVSGEILAVLYALEIAKQKGFKCITLYYDYEGLEKWITGEWRAKTPLTRVYKEKFYNYAKDIKVKFEKVKSHTGVKYNELADELAKYAVETNESNIDFEVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHI8</accession>
    <name>B7IHI8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methylenetetrahydrofolate reductase</fullName>
            <ecNumber evidence="8">1.5.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1096</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:19821"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19817"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.20" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101192.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1096"/>
    <dbReference type="EnsemblBacteria" id="ACJ75552">
        <property type="protein sequence ID" value="ACJ75552"/>
        <property type="gene ID" value="THA_1096"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1096"/>
    <dbReference type="eggNOG" id="COG0685">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025841_0_2_0"/>
    <dbReference type="OMA" id="EMHPQAR"/>
    <dbReference type="OrthoDB" id="1425269at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106312">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106313">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADPH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006555">
        <property type="term" value="P:methionine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="SSF51730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0274">FAD</keyword>
    <keyword evidence="5 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="305" mass="35338" checksum="0473FBA32AFB5F44" modified="2009-02-10" version="1">MKISQIKDFFISIEVIPPLRGHSADNLYKAIDKIMPYNPAFISVTKHPPELEYYEIDGQILKLPKVKRPGTMGIVAALKMRYNVDVVPHLTCIGNSKFELEELLIDLDFLGIDNVFVIRGDKKKNVIKKEWQYDYAYQLVQQISDMNKGKYLYQTAKKTNFCIGVAGYPEKHYESPNLKTDIKYLKRKIEAGAQFVITQMFFDVEKYFNFVKLLRDEEINVKVIPGLKPILSYKSALKIPGTFYVDIPEELIEKLDNARTPKEEFWTGVKYTIKLIEKLKENNVPGIHFFTMSNTDAIVEILKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHJ5</accession>
    <name>B7IHJ5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arginine deiminase</fullName>
            <shortName evidence="3">ADI</shortName>
            <ecNumber evidence="3">3.5.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Arginine dihydrolase</fullName>
            <shortName evidence="3">AD</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">arcA</name>
        <name evidence="5" type="ordered locus">THA_1106</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>H2O + L-arginine = L-citrulline + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:19597"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="EC" id="3.5.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the arginine deiminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.6" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580002.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1106"/>
    <dbReference type="EnsemblBacteria" id="ACJ75559">
        <property type="protein sequence ID" value="ACJ75559"/>
        <property type="gene ID" value="THA_1106"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1106"/>
    <dbReference type="eggNOG" id="COG2235">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052662_0_1_0"/>
    <dbReference type="OMA" id="ERATMHL"/>
    <dbReference type="OrthoDB" id="592329at2"/>
    <dbReference type="UniPathway" id="UPA00254">
        <property type="reaction ID" value="UER00364"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016990">
        <property type="term" value="F:arginine deiminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019547">
        <property type="term" value="P:arginine catabolic process to ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018101">
        <property type="term" value="P:protein citrullination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00242">
        <property type="entry name" value="Arg_deiminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003876">
        <property type="entry name" value="Arg_deiminase"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006356">
        <property type="entry name" value="Arg_deiminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01466">
        <property type="entry name" value="ARGDEIMINASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01078">
        <property type="entry name" value="arcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0056">Arginine metabolism</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Amidino-cysteine intermediate" evidence="3 4">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR006356-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="400" mass="46463" checksum="2BD8D01FFE08DDC0" modified="2009-02-10" version="1">MKVNVYSEVKKLKIVLLHRPGLELENIEPDLLEELLFEDIPYLKKAQQEHDYFAKALKDNGVEVKYVTDLLTNTLEKKGLKEQFVDEYLNLSEVKNEYILEALKEYLLSFETKEMVRKIISGVRIDEFKLNKKSFSTVVHLNKQFYLLPLPNLYFQRDPVAFVGRGALINRMKTKARRRESLFMKYVLKYSDEFSDIPIYYDMNEPFSIEGGDVLVLSDKVLAIGISQRTDPEAIEKVANKIFYESDESFDTILAINMPKKRAYMHLDTIFTMVSENKFLVHSKLETSLHVYVLKKNKHEFEVTEEDLQLEDVLKKYLERNDIELIKCGGDDEIAAKREQWNDGSNVLAISPGTVIAYERNYVTNQLLRERGVNVIEIPSSELSRGRGGPRCMSMPICRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHQ8</accession>
    <name>B7IHQ8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580055.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1171"/>
    <dbReference type="EnsemblBacteria" id="ACJ75622">
        <property type="protein sequence ID" value="ACJ75622"/>
        <property type="gene ID" value="THA_1171"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1171"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125323_0_0_0"/>
    <dbReference type="OMA" id="FYTTRKT"/>
    <dbReference type="OrthoDB" id="1776812at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016772">
        <property type="term" value="F:transferase activity, transferring phosphorus-containing groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="176" mass="19711" checksum="C9DC8717D9309FA8" modified="2009-02-10" version="1">MGLVTLADHVQDITENSIKAGAKNVTLEIYETDDEFTFIVADDGPGIKDVDKVFDPFYTTRSKEIRRFGLGLPFLKQAAEMTGGEVKIETKLGIGTKVYANFKKSHIDCQPVGDLTMVFLSLLMNQDVNLTIKRCRYNDCYEISSEVVKKYLGSLDSAEKINILKEMIEELEKQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHR1</accession>
    <name>B7IHR1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580058.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1174"/>
    <dbReference type="EnsemblBacteria" id="ACJ75625">
        <property type="protein sequence ID" value="ACJ75625"/>
        <property type="gene ID" value="THA_1174"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1174"/>
    <dbReference type="eggNOG" id="ENOG5033G0R">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_690649_0_0_0"/>
    <dbReference type="OMA" id="KNDRMIT"/>
    <dbReference type="OrthoDB" id="537153at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="394" mass="44277" checksum="518B2B3F80DA08AF" modified="2009-02-10" version="1">MKKLTVVFGVFLFGILLFSNALDFMPKEYDVVMYIPDISKLYDVVKQTNAGDILANQIGLEQMFTGVIEQQLMMQGYTLDDLDIFKELLIVAGKESTLLVLGPSKNTNKIKELFENFSGQSLPEEVKIIDGYFVVGENYGGGTLPEELEDFLNKGYLAVSYINTNEEDFKVKGFGYATLKGNEVEFHQEIVPQDDKTKQLFNDIASKDGIDILKDENIGGDLFGFINRKLPDSFLKSLPVDLPEVLEGFSGTAYICGDISSVLTNALSGMQVSNVPFYGVVYYNNPSWDMIEGEKRFENIGGKKYGIVETDEGTPILYINLSNEKITFYGVSPDEYVAGDKDFIKENYSSDYVVGLFVNLRPMIFNFIGIDSESYFKFYGYFKDGKLIEKAVLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHR3</accession>
    <name>GATB_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName evidence="1">Asp/Glu-ADT subunit B</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatB</name>
        <name type="ordered locus">THA_1176</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101374.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHR3"/>
    <dbReference type="SMR" id="B7IHR3"/>
    <dbReference type="STRING" id="484019.THA_1176"/>
    <dbReference type="PRIDE" id="B7IHR3"/>
    <dbReference type="EnsemblBacteria" id="ACJ75627">
        <property type="protein sequence ID" value="ACJ75627"/>
        <property type="gene ID" value="THA_1176"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1176"/>
    <dbReference type="eggNOG" id="COG0064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019240_0_0_0"/>
    <dbReference type="OMA" id="ARKWWMG"/>
    <dbReference type="OrthoDB" id="497127at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="Apn/Gln-ADT_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000117623" description="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B">
        <location>
            <begin position="1"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00121"/>
        </source>
    </evidence>
    <sequence length="476" mass="54374" checksum="7C51CC04A092B324" modified="2009-02-10" version="1">MRFKPVIGLEIHVQLNTKTKAFCSCPADVFELEPNSAICPVCTGQPGALPVPSKEMYEYGILLASALNCRINEYTRFDRKNYFYPDLPKGYQITQYFYPLANDGYLELDGEKIRINRIHLEEDAGKLVHSSEMITEAESSLVDMNRCGVPLAEIVTEPDIKSPEQARKFLEKLRQILRYLGVSTGDMEKGALRCDANISVIDTETNRQSNKVEVKNMNSFKFVEKALEFEFERIKNAMLEGKDVEKETRGWDLSTKTTISMRSKEEANDYRYFPEPDIPPVVLSSDEIKKIVEKMPELPDQKKERFMKDYNLSEYEANILTSSKGLADFYEECVEVTNDPKETSNWFLTELLKYESAESNFEELKIKPIHFKELFDLISSGKITRNIAKEVFEEVYKTGKSPKEVVKEKNIEVVGDASLIEEILKKMLEENPDKVEAYRNGKKGLLGFFVGGVMKQTKGKADPKVVNEIAKKLLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHR6</accession>
    <name>B7IHR6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580060.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1180"/>
    <dbReference type="EnsemblBacteria" id="ACJ75630">
        <property type="protein sequence ID" value="ACJ75630"/>
        <property type="gene ID" value="THA_1180"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1180"/>
    <dbReference type="eggNOG" id="ENOG5033BZH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195925_0_0_0"/>
    <dbReference type="OrthoDB" id="2081672at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="73" mass="8111" checksum="E68C2C7B3CD7B113" modified="2009-02-10" version="1">MRVNGINNMYSSVNTLQISSGKNVAANVKAPVMTKDQMAQLLSFMTYQQNKMNLKLVRIASQLYTGKSIDQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHS6</accession>
    <name>B7IHS6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chemotaxis protein CheA</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">THA_1190</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580070.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1190"/>
    <dbReference type="EnsemblBacteria" id="ACJ75640">
        <property type="protein sequence ID" value="ACJ75640"/>
        <property type="gene ID" value="THA_1190"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1190"/>
    <dbReference type="eggNOG" id="COG0643">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000650_3_6_0"/>
    <dbReference type="OMA" id="NELVECC"/>
    <dbReference type="OrthoDB" id="305000at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004105">
        <property type="entry name" value="CheA-like_dim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037006">
        <property type="entry name" value="CheA-like_homodim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037052">
        <property type="entry name" value="CheA-like_P2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010808">
        <property type="entry name" value="CheA_P2-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035891">
        <property type="entry name" value="CheY-binding_CheA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02895">
        <property type="entry name" value="H-kinase_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07194">
        <property type="entry name" value="P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01231">
        <property type="entry name" value="H-kinase_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00073">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55052">
        <property type="entry name" value="SSF55052"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HPt" evidence="13">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="11">
        <location>
            <begin position="299"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="CheW-like" evidence="12">
        <location>
            <begin position="551"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="130"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="269"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="8">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ75640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="680" mass="76716" checksum="644352078E7E5943" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHT8</accession>
    <name>B7IHT8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580078.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1205"/>
    <dbReference type="EnsemblBacteria" id="ACJ75652">
        <property type="protein sequence ID" value="ACJ75652"/>
        <property type="gene ID" value="THA_1205"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1205"/>
    <dbReference type="eggNOG" id="ENOG5032DWN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_648804_0_0_0"/>
    <dbReference type="OMA" id="VEVLWIR"/>
    <dbReference type="OrthoDB" id="1476577at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043748">
        <property type="entry name" value="DUF5693"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18949">
        <property type="entry name" value="DUF5693"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="428" mass="50233" checksum="807F2EEF4A3C3D97" modified="2009-02-10" version="1">MKIKDILSTSKSKTIFLSIFLIIGLIGALLRTNNDLKNMKVDIVFENSSKIEFFDGKNIKNKNAVYIIPKDATNINLEGINLNGKKFGILEFNISETISKEFAKNLSKDMVITVHYIKPEELSKYNEKTLFKRLWRAVVERSIDLIVLPKTPMTESVAKAFKNYFKISDASPYIPNIEFKYFFSTVLILFVLYLFPYAIFLLPTLYFSYEIFISLVSILGTVVIFFKIKDNVLKFFSYFTLGILTNLSLYDFEHLNNIKTYWGVKLSLVLLPSILLIQLIIKENKKIKSHLKFLIPLFTIFGIYYIIRSGNFGFVTDFERNIREFIEDLFIIRPRTKELLFYPLAFLIPYLKSNFYKKLSEIFASIAFLSTFNTFCHIRAPLFVNIYRELITLFLTLIIYSIFKIFFERGEYYEENKNSSHYRTGNRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHV8</accession>
    <name>RL5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplE</name>
        <name type="ordered locus">THA_1227</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101462.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHV8"/>
    <dbReference type="SMR" id="B7IHV8"/>
    <dbReference type="STRING" id="484019.THA_1227"/>
    <dbReference type="EnsemblBacteria" id="ACJ75672">
        <property type="protein sequence ID" value="ACJ75672"/>
        <property type="gene ID" value="THA_1227"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1227"/>
    <dbReference type="eggNOG" id="COG0094">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061015_2_1_0"/>
    <dbReference type="OMA" id="ERMYAFL"/>
    <dbReference type="OrthoDB" id="1456375at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_B">
        <property type="entry name" value="Ribosomal_L5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_L5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020930">
        <property type="entry name" value="Ribosomal_L5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_L5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_L5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_L5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="PTHR11994"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_1000142464" description="50S ribosomal protein L5">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <sequence length="181" mass="20683" checksum="9B8562C47090C113" modified="2009-02-10" version="1">MQYIPLKEKYENEIKPAMMKEFGYKNIHQVPRLEKIVINMGIGEGSRNKDVIDIHAKELALIAGQKPVVTKAKKSISNFKIRKGMPIGLKVTLRGVNMYNFLYKLINLVLPKVRDFRGLNPNGFDGRGNYSFGLTEQLVFPEISPDQVRRVQGMDIVIVTTAKTDDEARKLLELFGFPFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHX5</accession>
    <name>B7IHX5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trigger factor</fullName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">PPIase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">THA_1244</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FKBP-type PPIase family. Tig subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580085.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1244"/>
    <dbReference type="EnsemblBacteria" id="ACJ75689">
        <property type="protein sequence ID" value="ACJ75689"/>
        <property type="gene ID" value="THA_1244"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1244"/>
    <dbReference type="eggNOG" id="COG0544">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033058_3_1_0"/>
    <dbReference type="OMA" id="YTIEGFR"/>
    <dbReference type="OrthoDB" id="932993at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036953">
        <property type="entry name" value="GreA/GreB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005215">
        <property type="entry name" value="Trig_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008880">
        <property type="entry name" value="Trigger_fac_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037041">
        <property type="entry name" value="Trigger_fac_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008881">
        <property type="entry name" value="Trigger_fac_ribosome-bd_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036611">
        <property type="entry name" value="Trigger_fac_ribosome-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30560">
        <property type="entry name" value="PTHR30560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05698">
        <property type="entry name" value="Trigger_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05697">
        <property type="entry name" value="Trigger_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003095">
        <property type="entry name" value="Trigger_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102735">
        <property type="entry name" value="SSF102735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="Trigger_N" evidence="10">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Trigger_C" evidence="11">
        <location>
            <begin position="257"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ75689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="435" mass="51065" checksum="12EEC5F7890E2FDB" modified="2009-02-10" version="1">MEIKELGVDKNIVTKEFIFDSAEIQRAEKRTLNELNRKYTIEGFRKGKVPLSVFKARFGKDFYDYFVFEQLVEETYKSFEEESNLLLIPEIAEKEINSESARIVVNFHKKPVAKVDFEKIKVKVANKDQVLENYLDLRLKTLREENAVLEPKEGPAEFDDLVRVKTYVTNKETGKVLVDGNEDEYVLYKDDERPTIQNLVGHKKGDVVEFDREFQKGEEEKIVYNYKIEILDVYKRILPELTDEFVKNNLAEMHLETLDELKNKILEEGTKIYDDEIKHSIREQILAQLPEATELFISEKTIDYAVRLIVNNMKEEGKYEEYVKKYDSEDKAKEAIREYYVSELKKEAAIEKIAKEYNIERKATDEELEKYAEILAPYWGISVERAKVLVKERAEVRNEVENMIFVDKVLDKISENVEREIVDINKEGGEDEGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHZ1</accession>
    <name>B7IHZ1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Crispr-associated protein, family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580098.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1260"/>
    <dbReference type="EnsemblBacteria" id="ACJ75705">
        <property type="protein sequence ID" value="ACJ75705"/>
        <property type="gene ID" value="THA_1260"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1260"/>
    <dbReference type="eggNOG" id="ENOG502Z952">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027704_0_0_0"/>
    <dbReference type="OMA" id="FYTIDKP"/>
    <dbReference type="OrthoDB" id="709504at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013389">
        <property type="entry name" value="CRISPR-assoc_prot_Cas8b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013420">
        <property type="entry name" value="CRISPR-assoc_prot_Csh1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09484">
        <property type="entry name" value="Cas_TM1802"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02591">
        <property type="entry name" value="cas_Csh1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02556">
        <property type="entry name" value="cas_TM1802"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="637" mass="74680" checksum="E065C34AB0B4D11F" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>B7II02</accession>
    <name>B7II02_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1271</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1271"/>
    <dbReference type="EnsemblBacteria" id="ACJ75716">
        <property type="protein sequence ID" value="ACJ75716"/>
        <property type="gene ID" value="THA_1271"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1271"/>
    <dbReference type="HOGENOM" id="CLU_3259070_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="42" mass="4585" checksum="3AE98031F316D7AE" modified="2009-02-10" version="1">MERGKGVMKKIWKKVVVFALVALPFVISTVGNLTLWPGKGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7II06</accession>
    <name>B7II06_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA mismatch repair protein MutS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">mutS</name>
        <name evidence="10" type="ordered locus">THA_1275</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1275"/>
    <dbReference type="EnsemblBacteria" id="ACJ75720">
        <property type="protein sequence ID" value="ACJ75720"/>
        <property type="gene ID" value="THA_1275"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1275"/>
    <dbReference type="eggNOG" id="COG0249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002472_3_1_0"/>
    <dbReference type="OMA" id="TPMMAQY"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00096">
        <property type="entry name" value="MutS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005748">
        <property type="entry name" value="DNA_mismatch_repair_MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="PTHR11361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="SSF48334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="SSF53150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="SSF55271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01070">
        <property type="entry name" value="mutS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 7 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_MISMATCH_REPAIR_2" evidence="9">
        <location>
            <begin position="667"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="593"/>
            <end position="600"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="817" mass="94184" checksum="0E175BD2695138DB" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMV3</accession>
    <name>C0QMV3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase repeat family IS200</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_2027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580550.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2027"/>
    <dbReference type="EnsemblBacteria" id="ACN29520">
        <property type="protein sequence ID" value="ACN29520"/>
        <property type="gene ID" value="THA_2027"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2027"/>
    <dbReference type="eggNOG" id="COG1943">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101320_2_2_0"/>
    <dbReference type="OMA" id="HILFRAK"/>
    <dbReference type="OrthoDB" id="1547701at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002686">
        <property type="entry name" value="Transposase_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036515">
        <property type="entry name" value="Transposase_17_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01797">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01321">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143422">
        <property type="entry name" value="SSF143422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Y1_Tnp" evidence="1">
        <location>
            <begin position="10"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACN29520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="153" mass="18086" checksum="557EA1DC0E627204" modified="2009-05-05" version="1">MFLESNNHSVFKLNYHLVLVTKYRRSVIDDNISDFLKKIFEYIGEKYGIKLQEWNHDKDHIHVLFSAQPNSQLSKFINAYKSTSSRLVKKKFPQIKSKLWKSAFWSKSYCLLTVGGAPLEVLKEYIKSQGEDKRASNLQISYLSYERTRRKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICQ3</accession>
    <name>B7ICQ3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD superfamily (Subfamily IA) hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580154.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1335"/>
    <dbReference type="EnsemblBacteria" id="ACJ75780">
        <property type="protein sequence ID" value="ACJ75780"/>
        <property type="gene ID" value="THA_1335"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1335"/>
    <dbReference type="eggNOG" id="COG0637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_1_0"/>
    <dbReference type="OMA" id="DHTLWDF"/>
    <dbReference type="OrthoDB" id="1204073at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011951">
        <property type="entry name" value="HAD-SF_hydro_IA_YjjG/PynA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02254">
        <property type="entry name" value="YjjG/YfnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="225" mass="26705" checksum="C8EBF8E6D400B038" modified="2009-02-10" version="1">MKYEMIYFDLDNTILDFDKSEEYALKSVFEYLNIRYDESYIEIYRPINEKWWKLFSEGKYKKEVIVVERFREFFEKIGVFNLNFNEVAKVYLKGLSSVAFFIEGAEEFLEKLKEKGFRMAAITNGVESVQQKRAKIAKLDRFFEFVLTSEKVGKPKPEPDIFFYAEKLSNVPLSRSIYIGDNIETDYEGAKKAKLDFILFDPKNKFGNNIKKVENYKELYRALVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICU9</accession>
    <name>B7ICU9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">OsmC/Ohr family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1381</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101763.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1381"/>
    <dbReference type="EnsemblBacteria" id="ACJ75826">
        <property type="protein sequence ID" value="ACJ75826"/>
        <property type="gene ID" value="THA_1381"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1381"/>
    <dbReference type="eggNOG" id="COG1765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114057_1_2_0"/>
    <dbReference type="OMA" id="GPRPMEM"/>
    <dbReference type="OrthoDB" id="1430018at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="SSF82784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="141" mass="16060" checksum="E06F52732B1197DB" modified="2009-02-10" version="1">MPNASMNWYSGMLFYSKTTSGHDLILDANDTVGGKDAGARPKELILYSLMGCTGMDVVSLLRKMRVIDSLESFRMEVDFEVAQEHPKVYTKIHLKYIFKFNGEPPKDKVEKAVNLSQERYCAVSAMLKQVVPEFSHEIIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICX2</accession>
    <name>B7ICX2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101799.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1405"/>
    <dbReference type="EnsemblBacteria" id="ACJ75849">
        <property type="protein sequence ID" value="ACJ75849"/>
        <property type="gene ID" value="THA_1405"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1405"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085376_1_4_0"/>
    <dbReference type="OMA" id="YDSVVPH"/>
    <dbReference type="OrthoDB" id="1292067at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="177" mass="20556" checksum="E939A97C71A0204A" modified="2009-02-10" version="1">MKLGKKIKTLRIARGYTQEELADRCDLSRSFISQLENDQVSPSIDTLERILRVLGSDLKTFFSTDKRQEKIVFKASERVPMYLDEYGIKGYILMDNIEDKSIDPKLIELAPGAETEEEDPHEGDEFGYVISGKLELILDGKVYKVNQGDCFYYCSDRKHKIRNPYEKSVKFLWIDIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICY9</accession>
    <name>SECA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein translocase subunit SecA</fullName>
            <ecNumber evidence="1">7.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secA</name>
        <name type="ordered locus">THA_1422</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + cellular proteinSide 1 = ADP + phosphate + cellular proteinSide 2.</text>
            <dbReference type="EC" id="7.4.2.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer and homodimer. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Distribution is 50-50.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecA family.</text>
    </comment>
    <dbReference type="EC" id="7.4.2.8" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580218.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ICY9"/>
    <dbReference type="SMR" id="B7ICY9"/>
    <dbReference type="STRING" id="484019.THA_1422"/>
    <dbReference type="PRIDE" id="B7ICY9"/>
    <dbReference type="EnsemblBacteria" id="ACJ75866">
        <property type="protein sequence ID" value="ACJ75866"/>
        <property type="gene ID" value="THA_1422"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1422"/>
    <dbReference type="eggNOG" id="COG0653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005314_3_0_0"/>
    <dbReference type="OMA" id="MVHYDVQ"/>
    <dbReference type="OrthoDB" id="212453at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008564">
        <property type="term" value="F:protein-exporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18803">
        <property type="entry name" value="SF2_C_secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01382">
        <property type="entry name" value="SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000185">
        <property type="entry name" value="SecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020937">
        <property type="entry name" value="SecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011130">
        <property type="entry name" value="SecA_preprotein_X-link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044722">
        <property type="entry name" value="SecA_SF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011116">
        <property type="entry name" value="SecA_Wing/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036266">
        <property type="entry name" value="SecA_Wing/Scaffold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036670">
        <property type="entry name" value="SecA_X-link_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612">
        <property type="entry name" value="PTHR30612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01043">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07516">
        <property type="entry name" value="SecA_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00906">
        <property type="entry name" value="SECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00957">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00958">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81767">
        <property type="entry name" value="SSF81767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81886">
        <property type="entry name" value="SSF81886"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01312">
        <property type="entry name" value="SECA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_1000145071" description="Protein translocase subunit SecA">
        <location>
            <begin position="1"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="95"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="534"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01382"/>
        </source>
    </evidence>
    <sequence length="856" mass="99194" checksum="BAF4EE5DFFA85E39" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID09</accession>
    <name>B7ID09_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutamate synthase (NADPH), homotetrameric</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gltA</name>
        <name evidence="3" type="ordered locus">THA_1443</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580225.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1443"/>
    <dbReference type="EnsemblBacteria" id="ACJ75886">
        <property type="protein sequence ID" value="ACJ75886"/>
        <property type="gene ID" value="THA_1443"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1443"/>
    <dbReference type="eggNOG" id="COG0493">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_3_3_0"/>
    <dbReference type="OMA" id="QETQCEQ"/>
    <dbReference type="OrthoDB" id="671513at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006004">
        <property type="entry name" value="Glut_synth_NADPH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01316">
        <property type="entry name" value="gltA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fer4_20" evidence="2">
        <location>
            <begin position="17"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="140"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="457" mass="49979" checksum="8BF4AEC8EE540348" modified="2009-02-10" version="1">MAVKQRIETIERNPNERNKDFNEVNLGYTQELAIEEAKRCIQCPIPTCAQGCPVGIDIPGFIKKIAEKDFEGSYKVLKKYNSLPAICGRVCPQEIQCERSCVLNKVDQPISIGALERFIADWAINNNIEEDILPEKRINKKIAIIGAGPAGLTTASELSKAGFSVDMYEVFHDVGGVLIYGIPEFRLPKSIVKKEAQLLKKLGVNIFTNIPVGLAIHPKELLEKYDAIFIGVGAGTPKFMNIEGTELNGVYSANEFLTRINLMKAYKFPQNDTPVKIGEKIVVIGGGNTAMDAARSALRLGADVTVVYRRSENEMPARKEEIKHAKEEGVKFQTLTQPVRYIGDENGNLIGIECIKMELGEPDESGRRKPTPIPNSNFVIESTVAIEAIGTNPNKLLLSQFKGLELNKWGYIKADELGRTSIEKVFAGGDIVTGSATVILAMGAGKKAASAIKLALS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-26" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID25</accession>
    <name>RIMO_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal protein S12 methylthiotransferase RimO</fullName>
            <shortName evidence="1">S12 MTTase</shortName>
            <shortName evidence="1">S12 methylthiotransferase</shortName>
            <ecNumber evidence="1">2.8.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein S12 (aspartate-C(3))-methylthiotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ribosome maturation factor RimO</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rimO</name>
        <name type="ordered locus">THA_1459</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-SH + AH2 + L-aspartate(89)-[ribosomal protein uS12]-hydrogen + 2 S-adenosyl-L-methionine = 3-methylsulfanyl-L-aspartate(89)-[ribosomal protein uS12]-hydrogen + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:37087"/>
            <dbReference type="Rhea" id="RHEA-COMP:10460"/>
            <dbReference type="Rhea" id="RHEA-COMP:10461"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:29961"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:73599"/>
            <dbReference type="EC" id="2.8.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylthiotransferase family. RimO subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.4" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580238.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ID25"/>
    <dbReference type="SMR" id="B7ID25"/>
    <dbReference type="STRING" id="484019.THA_1459"/>
    <dbReference type="PRIDE" id="B7ID25"/>
    <dbReference type="EnsemblBacteria" id="ACJ75902">
        <property type="protein sequence ID" value="ACJ75902"/>
        <property type="gene ID" value="THA_1459"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1459"/>
    <dbReference type="eggNOG" id="COG0621">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_0_1_0"/>
    <dbReference type="OMA" id="HYAYPTG"/>
    <dbReference type="OrthoDB" id="397139at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103039">
        <property type="term" value="F:protein methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018339">
        <property type="term" value="P:peptidyl-L-beta-methylthioaspartic acid biosynthetic process from peptidyl-aspartic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01865">
        <property type="entry name" value="MTTase_RimO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005840">
        <property type="entry name" value="Ribosomal_S12_MeSTrfase_RimO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041582">
        <property type="entry name" value="RimO_TRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43837">
        <property type="entry name" value="PTHR43837"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18693">
        <property type="entry name" value="TRAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00274">
        <property type="entry name" value="ribosomal_protein_S12_methylth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01125">
        <property type="entry name" value="TIGR01125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000375048" description="Ribosomal protein S12 methylthiotransferase RimO">
        <location>
            <begin position="1"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="MTTase N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="131"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="363"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="427" mass="49077" checksum="2CF6060494C97769" modified="2009-02-10" version="1">MNFYVDVLGCPKNEADCALLKAYLEKKGNNIVNTIEDADAVVIDTCGFILEAKKESIEEILTYLELKKERDLKVYVTGCLVQRYGEELKKEIPEVDGWFGILPPEKIAENIGKESIIPKNPEPVYEFGGRVDEKQYAYVKISDGCDRACSFCTIPLFKGSFKSRKIDDIVKEVEYLILSGKKEIILVAQDTTGYGIDLYGKQMLPELLKRINDIPGDFWIRVMYMHPDHITDEIIEAFSYDKVLKYFDIPVQHGSDKVLKLMNRTKKSEHILKLVEKIRKRYEDAVLRTSIIVGFPGETDEDFEELLDFIKMVRFERLGAFIYSDEEEAPSYHFEGKVPEIVAQERLDILMEEQSKISFEINEKMVGKTFKVLFDEEEEGVLIARSYMDAPEIDGNIFVPGKFEEGFFKVKVTSADVYDLEGKIVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID41</accession>
    <name>B7ID41_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="3 4">N5-CAIR mutase</shortName>
            <ecNumber evidence="3 4">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 7" type="primary">purE</name>
        <name evidence="7" type="ordered locus">THA_1475</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="3 4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580251.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1475"/>
    <dbReference type="EnsemblBacteria" id="ACJ75918">
        <property type="protein sequence ID" value="ACJ75918"/>
        <property type="gene ID" value="THA_1475"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1475"/>
    <dbReference type="eggNOG" id="COG0041">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094982_2_2_0"/>
    <dbReference type="OMA" id="SNSIDGW"/>
    <dbReference type="OrthoDB" id="1701464at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 3 4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRC" evidence="6">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001338-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="150" mass="16036" checksum="95DFED9F2D8C9DFE" modified="2009-02-10" version="1">MVSIIAGSKSDSNIVEKVVKVLEEFSVPYETKYLSAHRTPDLLDEYIQSCEKKGTKVFIAIAGGAAHLPGVIASKTLKPVIGVPVKSNDLGGLDSLLSIVQMPNDIPVATVGIDRGKNAALLAIEILALSDESLSKKLKDYRSKLVGKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID69</accession>
    <name>B7ID69_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartate ammonia-lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102025.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1505"/>
    <dbReference type="EnsemblBacteria" id="ACJ75946">
        <property type="protein sequence ID" value="ACJ75946"/>
        <property type="gene ID" value="THA_1505"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1505"/>
    <dbReference type="eggNOG" id="COG1027">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021594_4_0_0"/>
    <dbReference type="OMA" id="EICENYV"/>
    <dbReference type="OrthoDB" id="734949at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lyase_1" evidence="1">
        <location>
            <begin position="9"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="454" mass="51024" checksum="0D19AE5A4FA528AD" modified="2009-02-10" version="1">MREEKDYLGKVEIEDDKLYGISSYRAHKLFPETGEKFDERFIWAYFMIKKAAATLNKELGYLDSNIADAIISTCDEWKTLKDHIIVDPLSGGAGTSVNMNINEVIANRASQLLNKEIGYVKPIEHVNLHQSTNDTFVTAGKIAIIVRLRELIDQIIKLQEIIQAKEKEFYRIRKVGRTQLMDGPPIMLGQEFGAWADALARDRWRLNKVEERIRNVNIGGTAIGTGIGAPKNYVLKIVNVLREICNVKIAKADNLIDATQNMDVFSEIHGLLKALSVNLYKISNDIRLLSSGPNTSIGELILPKVQIGSSIMPGKNNPVVPEYVMQISLVVFSHDSMINHASALGNLELNQFSPLIVHYTLKSINLLKNACYSLSNYIENIKANEERCRENLEKSISNLTPLINIFGYNEVSQAVKESNYDVDKAIKLLSEKFSISVEDIKKKINPDNLTKLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID93</accession>
    <name>B7ID93_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide transport ATP-binding protein AppD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1529</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102059.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1529"/>
    <dbReference type="EnsemblBacteria" id="ACJ75970">
        <property type="protein sequence ID" value="ACJ75970"/>
        <property type="gene ID" value="THA_1529"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1529"/>
    <dbReference type="eggNOG" id="COG0444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_0"/>
    <dbReference type="OMA" id="GSMPRID"/>
    <dbReference type="OrthoDB" id="1101128at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="7"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="336" mass="38083" checksum="AD3BA1DD03B1BB1C" modified="2009-02-10" version="1">MEPILSVRNLSTWFYMEEGVVKAVNDVSFDLHENEVVGIVGETGSGKSVTVKSIMRLIHKPGKIVNGQIIYRGRGKEEDLLKLSKDEIAEIRGKEISMIFQDPLTSLNPLYTIGDQLTETIIRHQNVDRKTAWEIGTEMLRKVQIPAPEKRMFAYPFEFSGGMRQRAVIAIALSCNPKVLIADEPTTALDVTIQAQILELMKDLQKEFKTGLLFITHDLGVIASMADRIIVMYGSRQMEIATAEDIFYNPLHPYTHMLLRAIPRLDKKQDKLEAIPGQPPRMIDVPNVCPFAPRCPRKLDKCTKELPELVELEPGHFVRCFNPVLDKKESEAMKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDA0</accession>
    <name>B7IDA0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1536</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102075.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1536"/>
    <dbReference type="EnsemblBacteria" id="ACJ75977">
        <property type="protein sequence ID" value="ACJ75977"/>
        <property type="gene ID" value="THA_1536"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1536"/>
    <dbReference type="eggNOG" id="COG2337">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111058_0_0_0"/>
    <dbReference type="OMA" id="SKMRIKR"/>
    <dbReference type="OrthoDB" id="1961695at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003477">
        <property type="entry name" value="PemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011067">
        <property type="entry name" value="Plasmid_toxin/cell-grow_inhib"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02452">
        <property type="entry name" value="PemK_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="181" mass="21447" checksum="187D675512F1D81D" modified="2009-02-10" version="1">MIFIQTLSNELKKYILEFWKQVKNLFNVYSDKQLISLFKWLNYFIEKLNYEHSYEPGKLPSFKRGDIVWVNLGHNIGSKLRGRRPVVILEAENNKKDKNIVIAPIRSYNSKGPKKIFEGLVYVGKHEILTKHSYVDLKHIRSISKMRIYRQNNKIIQGKLPNEILDEIDQKLIKLFTKINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDA2</accession>
    <name>B7IDA2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1538</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1538"/>
    <dbReference type="EnsemblBacteria" id="ACJ75979">
        <property type="protein sequence ID" value="ACJ75979"/>
        <property type="gene ID" value="THA_1538"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1538"/>
    <dbReference type="HOGENOM" id="CLU_3277930_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="41" mass="4791" checksum="137B00D286868C23" modified="2009-02-10" version="1">MVVNGNHETRIKHTVGLDPVELLCEDFGFPYESSWQQSKWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDB1</accession>
    <name>B7IDB1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Antitoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1549</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Antitoxin component of a type II toxin-antitoxin (TA) system.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phD/YefM antitoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102218.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1549"/>
    <dbReference type="EnsemblBacteria" id="ACJ75988">
        <property type="protein sequence ID" value="ACJ75988"/>
        <property type="gene ID" value="THA_1549"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1549"/>
    <dbReference type="eggNOG" id="COG4118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172543_0_0_0"/>
    <dbReference type="OMA" id="NVWELYL"/>
    <dbReference type="OrthoDB" id="2047723at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006442">
        <property type="entry name" value="Antitoxin_Phd/YefM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036165">
        <property type="entry name" value="YefM-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02604">
        <property type="entry name" value="PhdYeFM_antitox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143120">
        <property type="entry name" value="SSF143120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="86" mass="9990" checksum="FE93EFA84D750E6A" modified="2009-02-10" version="1">MNLKNKNIYYSIAEAKAKFSKVIDESRENNVIITKNGKPVSVIIDFDKFEKIVSFLENVWELYLLEIGDPSKFKDLNINDLFSDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDC7</accession>
    <name>B7IDC7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Oxygen-independent coproporphyrinogen III oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1566</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1566"/>
    <dbReference type="EnsemblBacteria" id="ACJ76004">
        <property type="protein sequence ID" value="ACJ76004"/>
        <property type="gene ID" value="THA_1566"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1566"/>
    <dbReference type="eggNOG" id="COG1243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057482_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="PTHR11135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="PTHR11135:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="296" mass="34378" checksum="8574258641F08ABD" modified="2009-02-10" version="1">MTGEKMPTKKELINTLSFFLDKSPEEIAFYGGTFTAIPLEKQKELIALVRKFYPDTPIRISTRPDELDYENLKFLKQNNVKTVEIGIQSMFDDVLSAANRGHSRKDNLNAVNLLKEFGFEISAHLMIGLLNDNIKKDLLSIQELLNLGVKMFRIHPTIIFKNTELEKLYLEGKYNPLTLDEAVNIASEMLLLIYQYGAKVIRLGYFVPENQKNQIVAGPYHPSFGDMTKSKAMNKIITRLNIKKVRYHKKFESWFNSYDNKQLEIEKYLSESNEIFFDNLSLKEASKRSFRRELLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDG7</accession>
    <name>PGK_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="1">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pgk</name>
        <name type="ordered locus">THA_1606</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580292.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDG7"/>
    <dbReference type="SMR" id="B7IDG7"/>
    <dbReference type="STRING" id="484019.THA_1606"/>
    <dbReference type="EnsemblBacteria" id="ACJ76044">
        <property type="protein sequence ID" value="ACJ76044"/>
        <property type="gene ID" value="THA_1606"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1606"/>
    <dbReference type="eggNOG" id="COG0126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_0"/>
    <dbReference type="OMA" id="DMIFDIG"/>
    <dbReference type="OrthoDB" id="90424at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00318">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PTHR11406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="SSF53748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000192858" description="Phosphoglycerate kinase">
        <location>
            <begin position="1"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="21"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="353"/>
            <end position="356"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <sequence length="402" mass="43454" checksum="EE1A6604DCB64D40" modified="2009-02-10" version="1">MEKLTIRDIDLKGKRVIMRVDFNVPMKDGEITDDTRIVEALPTIKYAIENGAIVILLSHLGRPKGERKPEFSLLPVAKRLSELLGKEVTFIPELYGEIVNEKVKNAKEGDVILLENTRFDKGETKNDPELAKKWAELADIHVNDAFGTAHRAHASNVGIAQYIPSVAGFLMEKEIKFLSKATTNPEKPYVVVLGGAKVSDKIGVITNLLDKADKILIGGAMMFTFLKSLGYNVGSSLVEDDKLDLAKRILEQAKEKGTEIVLPVDTVCAQKIEAGVETKTIDIKDGLPEGWMGLDIGPKTVELFRNSISGAKTIVWNGPMGVFEIEDFSNGTKEVAIAIAEETSKGATTIIGGGDSAAAIAKFGLKDKVSHVSTGGGASLEFLEGKELPGIASIANKKKIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDH5</accession>
    <name>B7IDH5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1614"/>
    <dbReference type="EnsemblBacteria" id="ACJ76052">
        <property type="protein sequence ID" value="ACJ76052"/>
        <property type="gene ID" value="THA_1614"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1614"/>
    <dbReference type="eggNOG" id="ENOG502ZW73">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1659899_0_0_0"/>
    <dbReference type="OMA" id="YMVLNNN"/>
    <dbReference type="OrthoDB" id="1888185at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032607">
        <property type="entry name" value="DUF4894"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16235">
        <property type="entry name" value="DUF4894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="165" mass="19890" checksum="AA72581009AC5F88" modified="2009-02-10" version="1">MDNYRYYFLWVIVFLILFIGIYNNFHLKKISAIDFSHKNSIAFKFILPYKNNLWVVSDSGKIIDIVEDYKVLKSLPIIVVPEEFVDGYFGKIKQDFLKKIPENIPSFVYEINFNENYMVLNNNARIYFNENFDFQMYFEKLKIVYNYIEPLGIYYISDEMLVKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDM0</accession>
    <name>B7IDM0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fervidolysin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1659</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580332.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76097">
        <property type="protein sequence ID" value="ACJ76097"/>
        <property type="gene ID" value="THA_1659"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1659"/>
    <dbReference type="HOGENOM" id="CLU_2439785_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="90" mass="9920" checksum="E141E9533763380B" modified="2009-02-10" version="1">MFYELITGIRGSYLSETFVSNMKYDFSSIADLVYLRLERSNVGATCTVNGTVTLNGTEIPVTAVFASEDATLSTVVDDVYGKPYWLIFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDM2</accession>
    <name>B7IDM2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Xanthine dehydrogenase iron-sulfur binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1661</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102471.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1661"/>
    <dbReference type="EnsemblBacteria" id="ACJ76099">
        <property type="protein sequence ID" value="ACJ76099"/>
        <property type="gene ID" value="THA_1661"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1661"/>
    <dbReference type="eggNOG" id="COG2080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052511_3_1_0"/>
    <dbReference type="OMA" id="FIKHDGY"/>
    <dbReference type="OrthoDB" id="1843415at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47741">
        <property type="entry name" value="SSF47741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="148" mass="16272" checksum="FD9DC6E8494707B5" modified="2009-02-10" version="1">MKISLIINGEKKDVEINPDDILLDVLRKLGYYSVRRGCDTGMCGICTVLLDGKPVPSCSVFAARADGHHITTVEGLEDAKVFAQYLAEEGADQCGFCSPGLIVNTVYLKNKNFKDKEEITNKLIGNLCRCTGYVGQHRAIEKFLGVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDN0</accession>
    <name>B7IDN0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Two-component response regulator variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580339.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1669"/>
    <dbReference type="EnsemblBacteria" id="ACJ76107">
        <property type="protein sequence ID" value="ACJ76107"/>
        <property type="gene ID" value="THA_1669"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1669"/>
    <dbReference type="eggNOG" id="COG2206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081823_0_0_0"/>
    <dbReference type="OMA" id="IAVCDYY"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD-GYP" evidence="1">
        <location>
            <begin position="79"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="270" mass="32018" checksum="EC1D7A28F126BA4E" modified="2009-02-10" version="1">MISKELSDFLREKLYDYDIGFEKGEKDINIFEENGFIFVEKDQDIVFSLKNEKRYFDLLPIIIEKVVRGHLNVLHKDKAEELIVDLLVNIIVLSEVEDSDGYSHTERVAKMAEKFGKFLGMDEKELRKFVFHAYLHDVGKISLEQLMLYSPTRMRLFEESYQDHTVMGSVFLSMFEVLWEYIPTVRYHHERWDGKGFPDGLRERQIPYYARVISILDFFDETTHFISSEWESELKTPKEAMEMIKKLSGKYFDPNLVEKFESFLKSEGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDN5</accession>
    <name>B7IDN5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ornithine decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1674</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Orn/Lys/Arg decarboxylase class-II family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102495.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1674"/>
    <dbReference type="EnsemblBacteria" id="ACJ76112">
        <property type="protein sequence ID" value="ACJ76112"/>
        <property type="gene ID" value="THA_1674"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1674"/>
    <dbReference type="eggNOG" id="COG0019">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026444_1_3_0"/>
    <dbReference type="OMA" id="SFFVCDL"/>
    <dbReference type="OrthoDB" id="861683at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482">
        <property type="entry name" value="PTHR11482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="3">
        <location>
            <begin position="29"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="2">
        <location>
            <begin position="263"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="387" mass="43384" checksum="06434071BDDC4927" modified="2009-02-10" version="1">MDKKILNKKLKALAEKHGTPILVMDLEVVRDNYKRLVENVKNSKIYYAVKANSHIEILKLLRDLGSYFDVASRPEIEKLLSIGVEPERMSFGNTIKKSSDIAFAYEKGIKMFAVDSEMEIEKIAKNAPGSDIYVRISTNGMEDDADWPLTKKFGTSVDHAISLVKYAYSLGLNPIGVSFHVGSQNYNPENWRVAIREVSVVFEEARRMGIEMKMVNTGGGMPVKYSKNIPTVEEISAVINEAIADYIGEDVTVILEPGRSMVGNAGTMITSVILRSQKGEEKWIYTDAGVFHGLTETIQNIRYEVEVIGKEDEEKEKFVLAGPTCDSVDVMYYDINLPKTTTMDDLVVLYNTGAYTTEYGTNFNGIPSPKIIFESSIFVKQELFEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDN8</accession>
    <name>B7IDN8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA modification GTPase MnmE</fullName>
            <ecNumber evidence="6">3.6.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mnmE</name>
        <name evidence="6 9" type="synonym">trmE</name>
        <name evidence="9" type="ordered locus">THA_1678</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="6">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.-.-" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580342.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1678"/>
    <dbReference type="EnsemblBacteria" id="ACJ76115">
        <property type="protein sequence ID" value="ACJ76115"/>
        <property type="gene ID" value="THA_1678"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1678"/>
    <dbReference type="eggNOG" id="COG0486">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019624_4_1_0"/>
    <dbReference type="OMA" id="CEIQCHG"/>
    <dbReference type="OrthoDB" id="263682at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04164">
        <property type="entry name" value="trmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00379">
        <property type="entry name" value="GTPase_MnmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031168">
        <property type="entry name" value="G_TrmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018948">
        <property type="entry name" value="GTP-bd_TrmE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004520">
        <property type="entry name" value="GTPase_MnmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027368">
        <property type="entry name" value="MnmE_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025867">
        <property type="entry name" value="MnmE_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT_dom1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12631">
        <property type="entry name" value="MnmE_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10396">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00450">
        <property type="entry name" value="mnmE_trmE_thdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51709">
        <property type="entry name" value="G_TRME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0630">Potassium</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TrmE-type G" evidence="8">
        <location>
            <begin position="211"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="221"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="240"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="265"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="441" mass="49348" checksum="16D87B377341382C" modified="2009-02-10" version="1">MFDTIAAISSPLGTGAISVVRIDGPKSHDIAKKLVKISNVDYRRVYHAFMEFEGQIVDEVNIVFYKSPNSYTGNDLVEIYGHGGILVTRKVLEAVLKSGARLAERGEFTKRAFLNGKISLVQAESIYQIIEAKSEISLKLSFENLKGKLSEEIENYRSRLLNILAEIEVSIDYPDDMEVDFDNIFNLLSKIDQELSLKIENSKKALTLNKGIVMAIVGKPNSGKSTLLNKLLEEDRAIVTDIPGTTRDVIKGEIDINGIHFVIVDTAGIRETDDVVESIGIQKSIKELEKADIVLFVLDSTTGFTKEDEYIFEKVKKLNYIPVWNKCDIGNNFNKEFKDAVKISALTGESFRSLESMILSKVSDIIESGESSHVITQRQLEVLERVKRNVESAIFNLKSGYEVDVVSIDIRKALEELDVLIGKRFSDDLLDTIFSNFCVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDP3</accession>
    <name>B7IDP3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutamate synthase, beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1683</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580346.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1683"/>
    <dbReference type="EnsemblBacteria" id="ACJ76120">
        <property type="protein sequence ID" value="ACJ76120"/>
        <property type="gene ID" value="THA_1683"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1683"/>
    <dbReference type="eggNOG" id="COG0493">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1143">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_3_3_0"/>
    <dbReference type="OMA" id="IDYGRCS"/>
    <dbReference type="OrthoDB" id="323168at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="42"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="181"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="616" mass="69620" checksum="4F7425F42A92BCB6" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDQ8</accession>
    <name>B7IDQ8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LUD_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1698"/>
    <dbReference type="EnsemblBacteria" id="ACJ76135">
        <property type="protein sequence ID" value="ACJ76135"/>
        <property type="gene ID" value="THA_1698"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1698"/>
    <dbReference type="eggNOG" id="COG1139">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107893_1_0_0"/>
    <dbReference type="OMA" id="RACRIYS"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003741">
        <property type="entry name" value="LUD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009501">
        <property type="entry name" value="UCP020269"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02589">
        <property type="entry name" value="LUD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF020269">
        <property type="entry name" value="DUF1121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LUD_dom" evidence="1">
        <location>
            <begin position="3"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="201" mass="22515" checksum="AD9BBD7F98FFB609" modified="2009-02-10" version="1">MANKVADALNKKGHEAYIVEDEKEALEKVLELIPKGSTVATGGSLTLNQIGLLEKLRTEEYNFLDRYSAKTKEEKEELERKAFFSDFYICSANAITENGEIALLDGNGNRVAAVIFGPKNVILVTSVNKVVKNLEEARERIRYISPMNTKRLNLNTPCAQTGYCVDCASHQRICNYYTIIESGYRHPGRIKVVIVLKELGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDR2</accession>
    <name>B7IDR2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8 10">Thymidine kinase</fullName>
            <ecNumber evidence="2 8 10">2.7.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tdk</name>
        <name evidence="12" type="ordered locus">THA_1702</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>ATP + thymidine = ADP + dTMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19129"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17748"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.21"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 11">Belongs to the thymidine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.21" evidence="2 8 10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102555.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1702"/>
    <dbReference type="EnsemblBacteria" id="ACJ76139">
        <property type="protein sequence ID" value="ACJ76139"/>
        <property type="gene ID" value="THA_1702"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1702"/>
    <dbReference type="eggNOG" id="COG1435">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064400_3_0_0"/>
    <dbReference type="OMA" id="ENTVNHK"/>
    <dbReference type="OrthoDB" id="1279539at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004797">
        <property type="term" value="F:thymidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00124">
        <property type="entry name" value="Thymidine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001267">
        <property type="entry name" value="Thymidine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020633">
        <property type="entry name" value="Thymidine_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11441">
        <property type="entry name" value="PTHR11441"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00265">
        <property type="entry name" value="TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035805">
        <property type="entry name" value="TK_cell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00603">
        <property type="entry name" value="TK_CELLULAR_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 10" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="6 8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8 9">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="83"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR035805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ76139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="186" mass="21015" checksum="25BF5AC91FD2EAD1" modified="2009-02-10" version="1">MVGKLTVITGPMYSGKTTELLSFVEIYNIGKKKTIVFKPSIDNRYGEDIVSTHTGFKVKAHKISKSIEILNHISNNIDAVFIDEVQFFDEELINVVRNLIFKGIDVYCAGLDISYLEKPFKTTANLLAIADEVIKKKAVCEKCGEHRATYSFKITQDDKEIDIGGKEKYIALCRECLKKIKVNKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDS0</accession>
    <name>B7IDS0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide chain release factor 1</fullName>
            <shortName evidence="5">RF-1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">prfA</name>
        <name evidence="9" type="ordered locus">THA_1711</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">Methylated by PrmC. Methylation increases the termination efficiency of RF1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102569.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1711"/>
    <dbReference type="EnsemblBacteria" id="ACJ76147">
        <property type="protein sequence ID" value="ACJ76147"/>
        <property type="gene ID" value="THA_1711"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1711"/>
    <dbReference type="eggNOG" id="COG0216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_0_1_0"/>
    <dbReference type="OMA" id="ISDHRVG"/>
    <dbReference type="OrthoDB" id="928964at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00093">
        <property type="entry name" value="Rel_fac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004373">
        <property type="entry name" value="RF-1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00019">
        <property type="entry name" value="prfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0488">Methylation</keyword>
    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RF_PROK_I" evidence="8">
        <location>
            <begin position="221"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="286"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="5">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="358" mass="41234" checksum="214F98F8A5D0CBAD" modified="2009-02-10" version="1">MENIEIVQQIHEWSKKLLKSLEEKLSSQLTPDQIRTYSEKYSKMKEIFQLAKEYLEIEDEIELWKEEMPEGFEHEVEKLQKKQEDIMQDLISLVLPESEYSGKNVFLEIRAGTGGEEAALFAADLLRMYLRYVEKKNFKAEIVDENKTDLGGYKEVVIKIKGKNVGNLLKYESGVHRVQRVPTTESGGRIHTSTATVAVLPEVSNVEIEINPSDIRIDTYRASGAGGQYVNKTESAVRITHIPTGIVVTCQSERSQLQNKEQAMNILRARLFKLKLEEQERKISSNRKSQIGTGERSEKIRTYNFPQNRVTDHRINYTSYNLQAVLDGELDEFITRLMRIDMLEQLENILKGGNEDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDT1</accession>
    <name>B7IDT1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transporter, cation:proton antiporter family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1722</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580372.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1722"/>
    <dbReference type="EnsemblBacteria" id="ACJ76158">
        <property type="protein sequence ID" value="ACJ76158"/>
        <property type="gene ID" value="THA_1722"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1722"/>
    <dbReference type="eggNOG" id="ENOG5032R91">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069132_3_0_0"/>
    <dbReference type="OMA" id="VFIRYRG"/>
    <dbReference type="OrthoDB" id="1836204at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007182">
        <property type="entry name" value="MnhB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04039">
        <property type="entry name" value="MnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="MnhB" evidence="8">
        <location>
            <begin position="85"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="200" mass="23328" checksum="E160BEFA040664CC" modified="2009-02-10" version="1">MAWIKSFSLIFLSILFFIFLFIESSYNLDFSRLGIVNNFVTEIYLKNRLYDTLFEVLVFSLAAMGIFLFNYSEERQKFHLHETHIRILLKFLSYFVILLSFYLAYYGDKTPGGGFSAGVVGGTGILLYSLSTTFNEIEYVFEKYKIKLFEKISIFIIYGFSISSLLGSVHVRLLSILIYLKVMFGMWIIIYSFIKHRGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDT8</accession>
    <name>B7IDT8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102606.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1730"/>
    <dbReference type="EnsemblBacteria" id="ACJ76165">
        <property type="protein sequence ID" value="ACJ76165"/>
        <property type="gene ID" value="THA_1730"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1730"/>
    <dbReference type="eggNOG" id="COG2035">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055621_2_1_0"/>
    <dbReference type="OMA" id="PIPVWSF"/>
    <dbReference type="OrthoDB" id="1426624at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007163">
        <property type="entry name" value="DUF368"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37308">
        <property type="entry name" value="PTHR37308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04018">
        <property type="entry name" value="DUF368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="250" mass="27269" checksum="CCB9A822ACF452CD" modified="2009-02-10" version="1">MRLFFAGILIGIANLIPGVSGGTMAVIAGVYEKLISAISNVLKFKKQDLKILIIVGLGVLVSIFSLSKFFEYSLQNFPFYMYSFFFGLIFASILFLKREVKLNIVPLILGILLVIIPNFIPHSSNSSILKIVIAGFAGGAAMVVPGLSGSLMLLLLGVYEYIISAISNLNIGILLIFGIGVLLGIYAVSLVMKIAFERYENFSKNLILGLVIGSLYPIYPGFHGSGNMIFGLFSAILGYFLSYMLSRYMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDX5</accession>
    <name>B7IDX5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580399.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76202">
        <property type="protein sequence ID" value="ACJ76202"/>
        <property type="gene ID" value="THA_1771"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1771"/>
    <dbReference type="HOGENOM" id="CLU_2720988_0_0_0"/>
    <dbReference type="OMA" id="IWRVIAI"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="72" mass="7832" checksum="983338C0CF1EE308" modified="2009-02-10" version="1">MINFLAILGVIFLVLLGIIFFPFLSPILSIVLGVLLIVLIFVGLVTIVFPVVLVIIAIALIVKGIKYIANKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE14</accession>
    <name>B7IE14_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580434.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1810"/>
    <dbReference type="EnsemblBacteria" id="ACJ76241">
        <property type="protein sequence ID" value="ACJ76241"/>
        <property type="gene ID" value="THA_1810"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1810"/>
    <dbReference type="eggNOG" id="ENOG5033NA4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186688_0_0_0"/>
    <dbReference type="OMA" id="MWMDKEK"/>
    <dbReference type="OrthoDB" id="1965858at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="89" mass="10777" checksum="E50AD1DF1B1A8635" modified="2009-02-10" version="1">MSEKDIKKSNFSENNYEMNWERYEKALSKVLQNFANRGYEKVTIEELWVETSLPKDLLLELIKERNIVFPEEIKEIKYKNDTIWSSNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE19</accession>
    <name>B7IE19_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Electron transport complex, rnfaBcdge type, b subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1815</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102752.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1815"/>
    <dbReference type="EnsemblBacteria" id="ACJ76246">
        <property type="protein sequence ID" value="ACJ76246"/>
        <property type="gene ID" value="THA_1815"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1815"/>
    <dbReference type="eggNOG" id="COG2878">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
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    <dbReference type="OMA" id="QAETNRC"/>
    <dbReference type="OrthoDB" id="1969005at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010207">
        <property type="entry name" value="Elect_transpt_cplx_RnfB/RsxB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01944">
        <property type="entry name" value="rnfB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S" evidence="6">
        <location>
            <begin position="31"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="93" mass="10008" checksum="4B9203C59DB5FD62" modified="2009-02-10" version="1">MVILYSALVMGALGLGFGLFLAFSNEKFKVEVDPRIEQITKVLPGINCGACGYPGCEGYASAIVKKGDAIDKCLPGKKMGVIERIKEILEQNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-01" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE23</accession>
    <name>PNP_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polyribonucleotide nucleotidyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polynucleotide phosphorylase</fullName>
            <shortName evidence="1">PNPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pnp</name>
        <name type="ordered locus">THA_1819</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + RNA(n+1) = a ribonucleoside 5'-diphosphate + RNA(n)</text>
            <dbReference type="Rhea" id="RHEA:22096"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the polyribonucleotide nucleotidyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.8" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580442.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IE23"/>
    <dbReference type="SMR" id="B7IE23"/>
    <dbReference type="STRING" id="484019.THA_1819"/>
    <dbReference type="EnsemblBacteria" id="ACJ76250">
        <property type="protein sequence ID" value="ACJ76250"/>
        <property type="gene ID" value="THA_1819"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1819"/>
    <dbReference type="eggNOG" id="COG1185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004217_2_2_0"/>
    <dbReference type="OMA" id="LHILDVM"/>
    <dbReference type="OrthoDB" id="122725at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004654">
        <property type="term" value="F:polyribonucleotide nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01595">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012162">
        <property type="entry name" value="PNPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015848">
        <property type="entry name" value="PNPase_PH_RNA-bd_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11252">
        <property type="entry name" value="PTHR11252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03726">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005499">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="SSF55666"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03591">
        <property type="entry name" value="polynuc_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000381925" description="Polyribonucleotide nucleotidyltransferase">
        <location>
            <begin position="1"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="559"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="629"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="492"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01595"/>
        </source>
    </evidence>
    <sequence length="697" mass="77669" checksum="2FD460DC02486705" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE36</accession>
    <name>B7IE36_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1832</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580452.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1832"/>
    <dbReference type="EnsemblBacteria" id="ACJ76263">
        <property type="protein sequence ID" value="ACJ76263"/>
        <property type="gene ID" value="THA_1832"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1832"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_902939_0_0_0"/>
    <dbReference type="OMA" id="RFINTHI"/>
    <dbReference type="OrthoDB" id="1554594at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="309" mass="35472" checksum="06EAE93E79E05740" modified="2009-02-10" version="1">MKKLNYNLYILIIIAIIFLQIIYWYFVQSNTYGFLYSLKNSSHNVVSSYDGKLKFISFNEDGFKKSDLRFLKSKGVNIIIGPNFSSSIAIIEKGLEKYGLIALSPSITSNDLLNNKNIFSFVPINKVQVDVIVTFLKEKNIKNVLLVLDPFNKIYSKDFLDILKTFNGKAVYFYSSRLSNIVLDNFDAIVITLSPNLALDFFNILSDFRGIVLLSDSAIDSSLRILPSYNNLYLVDFGFKQVDWSLITINEIINLLSKHKFISSEQFVSYFTSHTVVNNQAFTPEGYLIRDIHIEKFDILRKGFSIEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE43</accession>
    <name>B7IE43_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Potassium channel, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1839</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580454.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1839"/>
    <dbReference type="EnsemblBacteria" id="ACJ76270">
        <property type="protein sequence ID" value="ACJ76270"/>
        <property type="gene ID" value="THA_1839"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1839"/>
    <dbReference type="eggNOG" id="COG0569">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050982_0_0_0"/>
    <dbReference type="OMA" id="HIIVCGC"/>
    <dbReference type="OrthoDB" id="599322at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0407">Ion channel</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="2">
        <location>
            <begin position="116"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="3">
        <location>
            <begin position="256"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="340" mass="37928" checksum="90045914A4D01D62" modified="2009-02-10" version="1">MNRENIEKTLLKQIAFFSILILIVLLLGVLYYHFVEGLPFVDAFFFTAITISTVGYTMPENLSEAGRLFTSFLIFLGISVVLYGVSTVTAIIVEGKLSNYMKERRNRKMISKLKDHIIVVGAGKTGQYVIGELIREKENFVIIDLNEENINKVVEGYNISIPYIVGDAAEEDILLEAGVTKAKALITTLPEDHVNVFVVLSARTLNPTMTIISKVSDVASIRKLIYAGATTVVAAAEIAGTRMARLTTRPDSVNFLDLFAFGNESYRIEEVKIPPKSSIINKKLSELQLSKKFRIMVVAALRKGENIFGPDGDFEILEDDSLMILGRKEDIEEFKEFAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE79</accession>
    <name>B7IE79_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Flagellar biosynthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102879.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1880"/>
    <dbReference type="EnsemblBacteria" id="ACJ76306">
        <property type="protein sequence ID" value="ACJ76306"/>
        <property type="gene ID" value="THA_1880"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1880"/>
    <dbReference type="eggNOG" id="COG2257">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041013_4_2_0"/>
    <dbReference type="OMA" id="HEVPIYE"/>
    <dbReference type="OrthoDB" id="1823885at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006135">
        <property type="entry name" value="T3SS_substrate_exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029025">
        <property type="entry name" value="T3SS_substrate_exporter_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30531">
        <property type="entry name" value="PTHR30531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01312">
        <property type="entry name" value="Bac_export_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160544">
        <property type="entry name" value="SSF160544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0966">Cell projection</keyword>
    <keyword evidence="1" id="KW-0969">Cilium</keyword>
    <keyword evidence="1" id="KW-0282">Flagellum</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="75" mass="8489" checksum="C6F766859D2203E0" modified="2009-02-10" version="1">MALKYDPKVDFVPFVIAKGKEEVAKKILEIAKKSGVPIVKSSELVDELYKLKVLEEIPKKLYLAVAEIIVFLESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE88</accession>
    <name>B7IE88_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1889</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102893.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1889"/>
    <dbReference type="EnsemblBacteria" id="ACJ76315">
        <property type="protein sequence ID" value="ACJ76315"/>
        <property type="gene ID" value="THA_1889"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1889"/>
    <dbReference type="eggNOG" id="COG1655">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074582_1_0_0"/>
    <dbReference type="OMA" id="WVKREIP"/>
    <dbReference type="OrthoDB" id="606787at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018708">
        <property type="entry name" value="DUF2225"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09986">
        <property type="entry name" value="DUF2225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="221" mass="25827" checksum="C12E4D4BAFBBBF94" modified="2009-02-10" version="1">MNALWDKSYKCPVCGNEFYSKKVRIDSIKIASYDEDLKPNYLDVNPFLYEVVVCPNCLYAEYESIFEKDVKITEVEKIKSILGKLKSKLSNVKFTDERDIDTALKAYAIVVILSTLLKKYCKLADSYLKIAWLLREKGGEKEQEEISLAHALKNFENCYMNSDISDGRMEEKILFYLGELNRHFGNKSEAVKWFSKLMQKYKNSSSYYAKVGKDRWQSMRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE93</accession>
    <name>B7IE93_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Membrane bound protein LytR, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1894</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LytR/CpsA/Psr (LCP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102903.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1894"/>
    <dbReference type="EnsemblBacteria" id="ACJ76320">
        <property type="protein sequence ID" value="ACJ76320"/>
        <property type="gene ID" value="THA_1894"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1894"/>
    <dbReference type="eggNOG" id="COG1316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016455_5_2_0"/>
    <dbReference type="OMA" id="NEVMFYE"/>
    <dbReference type="OrthoDB" id="491753at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004474">
        <property type="entry name" value="LytR_CpsA_psr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03816">
        <property type="entry name" value="LytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00350">
        <property type="entry name" value="lytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="LytR_cpsA_psr" evidence="3">
        <location>
            <begin position="58"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="388" mass="45404" checksum="7AE5F2B72EB6FBE1" modified="2009-02-10" version="1">MNKNIVLLLIAIFVTLFSLGVVFLPWINILLSVIYSPLDEETNFLVLGLDKDIQNTRRTDVIMFLNINVKNREIKITNIPRDLIINNSKINSIYQKEGLGYLKELVENFVGKKIDKYVIVDYDVVKLVGDRIGPIEVYVDMPMKYTDYSQNLIIDFDPGIHYLYGEQLLAYMRFRKDSLGDIGRIEREKYIINQLIKAALKKDVFTLAKVFKEVYESMDTNIKTSELVFLATKFKDGFSIKSYSFPIKYDVNGNIYPGDLSKLKEFFSSKSLNKVDERYRFYIVNNTKNQNRTYNVNLHYMWKAAGYIPEDIYFMQNTNFLSDTVYIVNEEVKIEEVEKIVKTVHPKRNFKILLAKEDLENYLILIDTLTKNRNYPDLSVDFVVILSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEA2</accession>
    <name>B7IEA2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ion-translocating oxidoreductase complex subunit C</fullName>
            <ecNumber evidence="9">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Rnf electron transport complex subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rnfC</name>
        <name evidence="12" type="ordered locus">THA_1903</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the 4Fe4S bacterial-type ferredoxin family. RnfC subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580475.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1903"/>
    <dbReference type="EnsemblBacteria" id="ACJ76329">
        <property type="protein sequence ID" value="ACJ76329"/>
        <property type="gene ID" value="THA_1903"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1903"/>
    <dbReference type="eggNOG" id="COG4656">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010808_6_0_0"/>
    <dbReference type="OMA" id="YPMGSEK"/>
    <dbReference type="OrthoDB" id="688908at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00461">
        <property type="entry name" value="RsxC_RnfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010208">
        <property type="entry name" value="Ion_transpt_RnfC/RsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026902">
        <property type="entry name" value="RnfC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43034">
        <property type="entry name" value="PTHR43034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13375">
        <property type="entry name" value="RnfC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="SSF142019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01945">
        <property type="entry name" value="rnfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="11">
        <location>
            <begin position="354"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="11">
        <location>
            <begin position="394"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ76329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="438" mass="48198" checksum="B5BDAAB2119BC675" modified="2009-02-10" version="1">MKLSSFLGGVHPPENKSLSKDIPIKKAPLPEKVIVFMQQHAGAPAKPVVQVGDEVKTGQVIGEPSGFVSAYVHSPVTGKVVEVKKISNIIFGKAVDAVIIERESEDDWELLPHGDFEKFSKEELLDIIQKAGIVGLGGAMFPTHIKLNPPKDKKIDTLIINGAECEPYLTIDHRMMLEKHEEILLGIEIVKKILNVKNVYIGIEDNKIDAINLLNDKWRGKVTVVPLKTKYPQGAEKQLIYAVTKRSVPRGGLPLDVGVVVQNVSTMYAIKEAVIDGKPLMERGLTLTGEAIKKPGNWWIRIGTPISWIVDNLGEGFKEGLEEIKVLMGGPMMGIPINNIETPIVKGNNGITSIVPHEQKSTFCIRCSYCVNVCPMGLQPYLLDLLGKKKRYDEAASIGLMDCIECGSCTYICPAKIEHVKTIKLAKKVYRALRGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEH1</accession>
    <name>B7IEH1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">2-oxoacid ferredoxin oxidoreductase, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1977</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580522.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1977"/>
    <dbReference type="EnsemblBacteria" id="ACJ76398">
        <property type="protein sequence ID" value="ACJ76398"/>
        <property type="gene ID" value="THA_1977"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1977"/>
    <dbReference type="eggNOG" id="COG0674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_1_0_0"/>
    <dbReference type="OMA" id="GMPTRTQ"/>
    <dbReference type="OrthoDB" id="360732at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022367">
        <property type="entry name" value="2-oxoacid/accept_OxRdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03710">
        <property type="entry name" value="OAFO_sf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="13"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="POR_N" evidence="3">
        <location>
            <begin position="202"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="556" mass="61923" checksum="F9B1E0C7BC057ED0" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEI4</accession>
    <name>B7IEI4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, RpiR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1990</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1990"/>
    <dbReference type="EnsemblBacteria" id="ACJ76411">
        <property type="protein sequence ID" value="ACJ76411"/>
        <property type="gene ID" value="THA_1990"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1990"/>
    <dbReference type="eggNOG" id="COG1737">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055769_0_4_0"/>
    <dbReference type="OMA" id="QEDTEIY"/>
    <dbReference type="OrthoDB" id="992687at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05013">
        <property type="entry name" value="SIS_RpiR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000281">
        <property type="entry name" value="HTH_RpiR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035472">
        <property type="entry name" value="RpiR-like_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01418">
        <property type="entry name" value="HTH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51071">
        <property type="entry name" value="HTH_RPIR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH rpiR-type" evidence="1">
        <location>
            <begin position="17"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="126"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="290" mass="32314" checksum="F6AF8310863CF48A" modified="2009-02-10" version="1">MNLIRKVNRKDMEDVKLGIIEKLKAIYDQLKPAERKIARYIINYPDDVIHHSISELSELAKVGEATISRISRKLGYKGFQALKIELAKELSSISFEETKTNSVISEVIESLNKLSKIINENEINQIAKKVVESNKIFFFGVGSSGVVAEYGALLFTRGGFFANYYNDPHMQVISAAGMDNQTVVFGISSSGNIRDTVKSMKVAKDSKAFTVAITGGYESKITEVVDKTIYIPPAGLERGMPKISPIVSQISILHMIFEEVLSLKKEAIEIIKKANTTLKTKKYLSKNEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEM0</accession>
    <name>B7IEM0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyridoxal-5'-phosphate-dependent enzyme, beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_26</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579301.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_26"/>
    <dbReference type="EnsemblBacteria" id="ACJ74534">
        <property type="protein sequence ID" value="ACJ74534"/>
        <property type="gene ID" value="THA_26"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_26"/>
    <dbReference type="eggNOG" id="COG0031">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_592863_0_0_0"/>
    <dbReference type="OMA" id="TWVEQQG"/>
    <dbReference type="OrthoDB" id="345245at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PALP" evidence="3">
        <location>
            <begin position="80"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="472" mass="54400" checksum="0C0AA35CB47E522E" modified="2009-02-10" version="1">MKSKVPVIGPTFEEMLNPEKIDPKIRKRAIEMKKKDPLHPINLFNITWKDENNEIYYFEVPKEITGVDTNIIVLYAKEFPSGSHKVGATYSVLAEKTVYGEVDPLKHTLIWPSTGNYGIGGAWVSSRMNYRSIVLLPELMSKERFEIIKKYGAEVIATPGCESNVKEIYDKSKELYNKDPEHVRILNQFEEFGNYRFHYYVTGNTMIELVKNKKIGNSRISAVVLGVGSAGTIASGDRVKQEFSKAKVVAVEPLQCPTISLNGYGGHDIQGIGDKHVTWIHNVMNNDAVVLVDDMESKKMLQVLSDPVGIEFLKEFAPKDMVDFISDKFGISGVANLIGAIKIAKYYNFTSDDNIFIVATDSIERYYSVMKDLEEKFGKLDRAEAKARTERILLYKEPSWVFEGDKYSRERWHNLKYYTWVEQQGKTVEELNAQKDQSYWIKQQEKVYEVNEKILEYRNKHYDELMKIYFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEN1</accession>
    <name>B7IEN1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_37</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2019" name="Biochim Biophys Acta Proteins Proteom" volume="1867" first="426" last="433">
            <title>Structure and function of the putative thioredoxin 1 from the thermophilic eubacterium Thermosipho africanus strain TCF52B.</title>
            <authorList>
                <person name="Sahtout N."/>
                <person name="Kuttiyatveetil J.R.A."/>
                <person name="Sanders D.A.R."/>
            </authorList>
            <dbReference type="PubMed" id="30716506"/>
            <dbReference type="DOI" id="10.1016/j.bbapap.2019.01.011"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS)</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103357.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="PDB" id="6MOS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A=1-90"/>
    </dbReference>
    <dbReference type="PDBsum" id="6MOS"/>
    <dbReference type="SMR" id="B7IEN1"/>
    <dbReference type="STRING" id="484019.THA_37"/>
    <dbReference type="EnsemblBacteria" id="ACJ74545">
        <property type="protein sequence ID" value="ACJ74545"/>
        <property type="gene ID" value="THA_37"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_37"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_16_1_0"/>
    <dbReference type="OMA" id="KRFARNF"/>
    <dbReference type="OrthoDB" id="1630944at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="6"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="6MOS"/>
        </source>
    </evidence>
    <sequence length="90" mass="10531" checksum="5F2F7B97B5F5AB94" modified="2009-02-10" version="1">MKIEYFKNDKCSVCKAMLPKIQTIAKNFDIDIEVIDVIENPSYPAQKLVFTVPTVIILDKEFEIKRFARNFSISEVINTIERYLEISNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEN3</accession>
    <name>B7IEN3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Iron (II) transport protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_39</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103358.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_39"/>
    <dbReference type="EnsemblBacteria" id="ACJ74547">
        <property type="protein sequence ID" value="ACJ74547"/>
        <property type="gene ID" value="THA_39"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_39"/>
    <dbReference type="eggNOG" id="COG1918">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143561_0_0_0"/>
    <dbReference type="OMA" id="MGDPRMY"/>
    <dbReference type="OrthoDB" id="2013450at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeoA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="FeoA" evidence="2">
        <location>
            <begin position="77"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="142" mass="15957" checksum="2D6F0A05D67D5F86" modified="2009-02-10" version="1">MTLDKIPECVKVKIVSIKDSKIKNRLLGLGLIPESIVEVVRTSPMGDPRMYRVFNKLISMRNSEASTIEVVLLDDSIPLSLANDGEYEVVEFCGGRRFLYKVSKLGILPGKIIQVKDGEIFLDNSKIHLGEGMKNKIILRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEU5</accession>
    <name>B7IEU5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Udk phosphoribulokinase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579347.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_101"/>
    <dbReference type="EnsemblBacteria" id="ACJ74609">
        <property type="protein sequence ID" value="ACJ74609"/>
        <property type="gene ID" value="THA_101"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_101"/>
    <dbReference type="eggNOG" id="COG0441">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0572">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023775_1_0_0"/>
    <dbReference type="OMA" id="DDHNRIP"/>
    <dbReference type="OrthoDB" id="1793376at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PRK" evidence="2">
        <location>
            <begin position="295"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="558" mass="64658" checksum="F557F4470073F00F" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF43</accession>
    <name>B7IF43_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DRTGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103723.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_202"/>
    <dbReference type="EnsemblBacteria" id="ACJ74707">
        <property type="protein sequence ID" value="ACJ74707"/>
        <property type="gene ID" value="THA_202"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_202"/>
    <dbReference type="eggNOG" id="COG4109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140224_0_0_0"/>
    <dbReference type="OMA" id="CVRTAGV"/>
    <dbReference type="OrthoDB" id="1858096at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010766">
        <property type="entry name" value="DRTGG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028979">
        <property type="entry name" value="Ser_kin/Pase_Hpr-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07085">
        <property type="entry name" value="DRTGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75138">
        <property type="entry name" value="SSF75138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DRTGG" evidence="1">
        <location>
            <begin position="5"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="116" mass="12597" checksum="9FE2EA507C3595A7" modified="2009-02-10" version="1">MKLKEIINLLNAKVIVDNGIENIEVEKVAASDLMSDVLAFGEEGSLLVTGLASPQCVRTASVVGIPAVIIVRNREIMPETIKIAELNGISLILTKYGMFETCGILYKHGLKPINKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF45</accession>
    <name>RNH2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease HII</fullName>
            <shortName evidence="1">RNase HII</shortName>
            <ecNumber evidence="1">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnhB</name>
        <name type="ordered locus">THA_204</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase HII family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579411.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IF45"/>
    <dbReference type="SMR" id="B7IF45"/>
    <dbReference type="STRING" id="484019.THA_204"/>
    <dbReference type="EnsemblBacteria" id="ACJ74709">
        <property type="protein sequence ID" value="ACJ74709"/>
        <property type="gene ID" value="THA_204"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_204"/>
    <dbReference type="eggNOG" id="COG0164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036532_3_2_0"/>
    <dbReference type="OMA" id="YPTKLHL"/>
    <dbReference type="OrthoDB" id="1685277at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07182">
        <property type="entry name" value="RNase_HII_bacteria_HII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00052_B">
        <property type="entry name" value="RNase_HII_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022898">
        <property type="entry name" value="RNase_HII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="PTHR10954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF18">
        <property type="entry name" value="PTHR10954:SF18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000116851" description="Ribonuclease HII">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <sequence length="220" mass="25084" checksum="A7BBC74DF0083AC7" modified="2009-02-10" version="1">MIVAGVDEAGRGPLFGPVVAAAVILNEEIDGLDDSKKLTSRKREKLFLEIIKKSYFAISIATADEIDKFNILHATEIAMDRALERLRKKINFDIAFVDGKNLNLKFPFKCIVKGDSLVKEISAASILAKVVRDRIIVSYAKKFSSYMLEKHKGYPTQLHLKLIKEHGLTPLHRLTYKPVMELLSITQLEEWYKSGMIDEERYKKIISKMEVPLFGIQDTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF93</accession>
    <name>B7IF93_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Iron (III) ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_255</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579447.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_255"/>
    <dbReference type="EnsemblBacteria" id="ACJ74757">
        <property type="protein sequence ID" value="ACJ74757"/>
        <property type="gene ID" value="THA_255"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_255"/>
    <dbReference type="eggNOG" id="COG0609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_0_1_0"/>
    <dbReference type="OMA" id="MQGVFGN"/>
    <dbReference type="OrthoDB" id="1283908at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="325" mass="35374" checksum="DBC7E718E1349CD6" modified="2009-02-10" version="1">MTKINIYKTGRIILPVLFFLVILINLSIGTVKIPISQIFNPENEKIKFLIWNVRFPRILMSLIAGSALHLLETSTQTIMKNPLVDPYLIGTSSGAGFGAVLALYVAVSLNRTVSVNVFSFAFSLLASLLTIILAKRKRIIPITHLILSGVLISTIFSSATILLINLSRKTLTSAYVWLFGSLSGTTINDLTIPAISVIVLFFIALIKNRELDAIALGEEEAKILGVDVEKIKWFFYLAGSFVISVIVSKTGIIGFVGLIIPHAARILTGSKHKYSLIGSILLGGIFLSFSDTISRTIASPIEVPIGVVTSFIGAPLMFILLKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF94</accession>
    <name>B7IF94_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NagD protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_256</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579448.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_256"/>
    <dbReference type="EnsemblBacteria" id="ACJ74758">
        <property type="protein sequence ID" value="ACJ74758"/>
        <property type="gene ID" value="THA_256"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_256"/>
    <dbReference type="eggNOG" id="COG0647">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043473_1_2_0"/>
    <dbReference type="OMA" id="PPMHRET"/>
    <dbReference type="OrthoDB" id="1308416at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="267" mass="29463" checksum="C66C1E8E74D5EA62" modified="2009-02-10" version="1">MKILIKDSLKKLENVKLFVLDIDGTFSLSGKLFPGSIEFANTVLKANKKFVFLTNNSNKSIDDYLLEFKNAGLSINADQIFTAGIETAEYLIEKFGPKKIYLVGNKAIKQVFENIGHKVVDSEEPDIVVVTFDTTLNYEKLAKACNYVSKGKLFVLTNPDLNCPSKDGPIPDTGAIASIITKATGKEPDIIFGKPDPLILEMIMQKNNVKKEETCVIGDRLYTDILLGKRAGVLTILVLTGEAKESDIKNIKPDIIAQEIGEISKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF97</accession>
    <name>B7IF97_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">GTP cyclohydrolase-2</fullName>
            <ecNumber evidence="12">3.5.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">GTP cyclohydrolase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ribB</name>
        <name evidence="12" type="synonym">ribA</name>
        <name evidence="14" type="ordered locus">THA_260</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + formate + 3 H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:23704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the DHBP synthase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.25" evidence="12"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579451.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_260"/>
    <dbReference type="EnsemblBacteria" id="ACJ74761">
        <property type="protein sequence ID" value="ACJ74761"/>
        <property type="gene ID" value="THA_260"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_260"/>
    <dbReference type="eggNOG" id="COG0108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_1_2_0"/>
    <dbReference type="OMA" id="ECRGLIC"/>
    <dbReference type="OrthoDB" id="900513at2"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00179">
        <property type="entry name" value="RibA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
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        <property type="entry name" value="RibA"/>
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        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF142695"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ribA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 12 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP_cyclohydro2" evidence="13">
        <location>
            <begin position="183"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="224"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="266"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ74761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="374" mass="43077" checksum="65152B0E16EEC2E8" modified="2009-02-10" version="1">MKPYILIDKKNELEGDFFIPAQICDEKILNFIIEKGKGLLCVSAHEDDLLKRGFFKLPSNGSKTNFFITVDYGKGTGISISERCNTIQKISEGLSIKDFVYPGHVNVLGAKEKRWGHTEASVFFSEHLGYKPYGVIIEILNSNGDSHDFEYILELSKKNKLEIIDIEDLYKEYVKKRFSIDTSVKLPTAIDEDFDIYTFKNPYDSREHIAIVKRPFKESIPYLRIHSECATGDIFHSLRCDCGEQLENSLKIISKKGGVLIYLRQEGRGIGLNNKVKAYKLQDEGYDTVEANLKLGFKDDERDYFAAYLILKALKINEVKILTNNPKKYEDLKRYGIKIKEIVNIYGKVNKYNKKYLFTKAKKMKHNLKLEGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFA1</accession>
    <name>B7IFA1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SUF system FeS assembly protein, NifU family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579455.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_264"/>
    <dbReference type="EnsemblBacteria" id="ACJ74765">
        <property type="protein sequence ID" value="ACJ74765"/>
        <property type="gene ID" value="THA_264"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_264"/>
    <dbReference type="eggNOG" id="COG0822">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079283_4_0_0"/>
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    <dbReference type="OrthoDB" id="1793654at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="PTHR10093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NifU_N" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="132" mass="15214" checksum="B8D49A22821CB58A" modified="2009-02-10" version="1">MYNELIMDYSKLKKYKGKIEDATKVEEGKNLSCGDEITLYLKIENERIVDAKFEGIGCAISQASANLMIENIINKSIDDVKILIDNVYKMTRGEEYDKSVLGLIEELKSIKDYPMRIKCFLLAWKTLELSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFC3</accession>
    <name>B7IFC3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative acyltransferase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579471.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_287"/>
    <dbReference type="EnsemblBacteria" id="ACJ74787">
        <property type="protein sequence ID" value="ACJ74787"/>
        <property type="gene ID" value="THA_287"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_287"/>
    <dbReference type="eggNOG" id="COG3594">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_811171_0_0_0"/>
    <dbReference type="OMA" id="VHAGTPN"/>
    <dbReference type="OrthoDB" id="1547996at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl_transf_3" evidence="2">
        <location>
            <begin position="4"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="326" mass="38581" checksum="A46E45C2E52F3697" modified="2009-02-10" version="1">MRIREIDVSRGILILMIIFVHSYIPYSLAVYFSYILASFMFISGYLFKDGKFSKKFEKILLNLLIPFVFLSLVGYIIYYILNIFIGYSNSVFSNFLDFVIFGYSTFDVPVNVLPLWYLYMFAVAELIFILFIKLRTIHFIPILSILSTLLINEQTKFFKINVALHGLIWFYLGYILKNRGFKFKFKNPFLLFIIFSCLLAVVATFNGFDDWRINSYGNYPLLSYVGEFSSVIIIISLAQMIKSDKIKRFFELFGKNTIFLLGYHIVLPGTLAIFIKDPVQFLEKYWFIYYILAVFILYLFLKIVPENLIYLLEGRFYFIEEFIKYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFD4</accession>
    <name>B7IFD4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">D-alanine-D-alanine ligase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_298</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579480.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_298"/>
    <dbReference type="EnsemblBacteria" id="ACJ74798">
        <property type="protein sequence ID" value="ACJ74798"/>
        <property type="gene ID" value="THA_298"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_298"/>
    <dbReference type="eggNOG" id="COG1181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039268_2_0_0"/>
    <dbReference type="OMA" id="IDFFLTD"/>
    <dbReference type="OrthoDB" id="764798at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008716">
        <property type="term" value="F:D-alanine-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011095">
        <property type="entry name" value="Dala_Dala_lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07478">
        <property type="entry name" value="Dala_Dala_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="4">
        <location>
            <begin position="96"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="312" mass="35952" checksum="28CA67C69F84C2A5" modified="2009-02-10" version="1">MKIAIVCDKNINDEEKRMVEAVKNAVSKKYDCEVVQFDENFINKIKGFDFVFNLSNKGGKETIQVHVPALLDLLNIPYTSSNAYSHSLCLDKITTKIIMQHYNIPTPRFYVYDLGQTPEKIDNGTFIVKPPREGSARGISKDSVVDNLKALQKMVKYIHEEFKQPALVEEFIDGKELSVGIIGNGDSLEVLPILEIDFSTLPEGLERFYSYNVKHNYGEMTNYICPARISNNVKEKLEFYAKKLFRVLNLRDYARMDVRIRDDEIYFLEVNSAPQLVPVYSDITKMAKAAGYEYDDLILKILEISMERWELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFF9</accession>
    <name>B7IFF9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endoribonuclease Cas2</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cas2</name>
        <name evidence="10" type="ordered locus">THA_323</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas1 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103961.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_323"/>
    <dbReference type="EnsemblBacteria" id="ACJ74823">
        <property type="protein sequence ID" value="ACJ74823"/>
        <property type="gene ID" value="THA_323"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_323"/>
    <dbReference type="eggNOG" id="COG1343">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161124_0_1_0"/>
    <dbReference type="OMA" id="IMRVINT"/>
    <dbReference type="OrthoDB" id="1951170at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09725">
        <property type="entry name" value="Cas2_I_II_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405">
        <property type="entry name" value="PTHR34405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="87" mass="10459" checksum="1ED9468D3EDCEB89" modified="2009-02-10" version="1">MYLIMIYDVNKKRVGKVLKVARKYLKWVQNSVLEGKITKSNFETLRNQILSIIDEDEDTIYWYILEPEFVPYRKIYGNKKINVTNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFI1</accession>
    <name>B7IFI1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">D-3-phosphoglycerate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">serA</name>
        <name evidence="6" type="ordered locus">THA_345</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579513.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_345"/>
    <dbReference type="EnsemblBacteria" id="ACJ74845">
        <property type="protein sequence ID" value="ACJ74845"/>
        <property type="gene ID" value="THA_345"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_345"/>
    <dbReference type="eggNOG" id="COG0111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_3_0"/>
    <dbReference type="OMA" id="VHHQTLG"/>
    <dbReference type="OrthoDB" id="1638924at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="4">
        <location>
            <begin position="6"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="5">
        <location>
            <begin position="104"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="303" mass="33321" checksum="81D4037BEC4B7A74" modified="2009-02-10" version="1">MRIHVNDPLDKSAMERLKNSGYNVTSEHLEKEELIKEIPNIDVLVVRSATKVTADIIEAGKNLKIIARAGTGLDNVDVEKAKEKGIKVINTPGANGISVAELAIGLMISCARHIAKGTIDLKNGEWTKKQLKGHELYKRTVGIIGFGNIGREVAKRLLAFDMRVLAYDPFVKETDMNVEIVDLDTIFKESDFITIHVPLTNETKHLVSKDAFEKMKDGVILINAARGGVVDEEALYNALISGKVYAAGLDVFEVEPPTDELRKKLLELPNVVATPHIGASTVEAQLRVGQIIVDKILEEVKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFL7</accession>
    <name>B7IFL7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Appr-1-p processing domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_388"/>
    <dbReference type="EnsemblBacteria" id="ACJ74881">
        <property type="protein sequence ID" value="ACJ74881"/>
        <property type="gene ID" value="THA_388"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_388"/>
    <dbReference type="eggNOG" id="COG2110">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046550_7_0_0"/>
    <dbReference type="OMA" id="HYGKGLP"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="181" mass="19983" checksum="AD0ADA90BD8AB10C" modified="2009-02-10" version="1">MINLNGKKIVLINDDITIQDTDAIVNAANSHLKHGGGVAGAILKKGGRIIQIESDEYVEKYGPVKTGNVAVTSGGNLKTKYIIHAVGPIWRGGNNNEEKLLKDAVLNSLKKADELKIKSISFPAISSGIFGFPVRRCAQIFAEAIDEFLKDNTTIEEIRIVNIDKDTHQVFEEEFKKYFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFM1</accession>
    <name>B7IFM1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type-4 uracil-DNA glycosylase</fullName>
            <ecNumber evidence="3">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_392</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 4 (UDGa) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104121.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_392"/>
    <dbReference type="EnsemblBacteria" id="ACJ74885">
        <property type="protein sequence ID" value="ACJ74885"/>
        <property type="gene ID" value="THA_392"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_392"/>
    <dbReference type="eggNOG" id="COG1573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044815_1_3_0"/>
    <dbReference type="OMA" id="RPWLIAE"/>
    <dbReference type="OrthoDB" id="1529383at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005273">
        <property type="entry name" value="Ura-DNA_glyco_family4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00758">
        <property type="entry name" value="UDG_fam4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDG" evidence="9">
        <location>
            <begin position="31"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="199" mass="22646" checksum="2CFAA7F24C8B2CAC" modified="2009-02-10" version="1">MTKKEMMDIIVERIKNCKACPLHENRTNVVPGEGNLDSPIMFVGEGPGEDEDLSGRPFVGRAGQLLTKILESVNIKREDVYITNIVKCRPPNNRTPTKEEASICSHYLFAQIEVINPRMIVTLGKPALELFLGKSVAITKVRGNEFEWKGGIIIFPMFHPSYLLRNPSKAKGSPKDLTWQDIKRVRKYYDQFLKERENG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-22" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFM5</accession>
    <name>KPRS_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="1">RPPK</shortName>
            <ecNumber evidence="1">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-phospho-D-ribosyl alpha-1-diphosphate</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="1">P-Rib-PP synthase</shortName>
            <shortName evidence="1">PRPP synthase</shortName>
            <shortName evidence="1">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">prs</name>
        <name type="ordered locus">THA_396</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579546.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFM5"/>
    <dbReference type="SMR" id="B7IFM5"/>
    <dbReference type="STRING" id="484019.THA_396"/>
    <dbReference type="PRIDE" id="B7IFM5"/>
    <dbReference type="EnsemblBacteria" id="ACJ74889">
        <property type="protein sequence ID" value="ACJ74889"/>
        <property type="gene ID" value="THA_396"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_396"/>
    <dbReference type="eggNOG" id="COG0462">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033546_2_0_0"/>
    <dbReference type="OMA" id="FGWARQD"/>
    <dbReference type="OrthoDB" id="1011035at2"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_B">
        <property type="entry name" value="RibP_PPkinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037515">
        <property type="entry name" value="Rib-P_diPkinase_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="PTHR10210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000212141" description="Ribose-phosphate pyrophosphokinase">
        <location>
            <begin position="1"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="41"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="100"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="228"/>
            <end position="232"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00583"/>
        </source>
    </evidence>
    <sequence length="316" mass="34785" checksum="1480E87A45F99B01" modified="2009-02-10" version="1">MQIAKNEMKIFSGNANRELAIKVSEYIGTRLADCEVGRFADGEINVKIGETVRGHDTFIIQPTCPPVNENLMELLIMIDALKRASANSIAVVIPYYGYARQDRKAKGRDPITAKLVANLLTVAGATRVMTVDLHSEQIQGFFDIPLDNLWSFPIFAKKLKEDKIVDDDYVIVSPDVGGVKRARQFAERLGGPLAILDKRRPKDNVAEILNIIGEVEGKTAIIVDDIADTARSLVNAAKAIKEKGAKRVIACITHPVLSDGAIERIQNSEIEKIYISDSISHSNLPDKFSVVSLAPLLGEAIVRVRKNLSISILFRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFQ8</accession>
    <name>B7IFQ8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-lysine 5,6-aminomutase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579574.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_429"/>
    <dbReference type="EnsemblBacteria" id="ACJ74922">
        <property type="protein sequence ID" value="ACJ74922"/>
        <property type="gene ID" value="THA_429"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_429"/>
    <dbReference type="eggNOG" id="COG5012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065773_0_0_0"/>
    <dbReference type="OMA" id="AIMNMKG"/>
    <dbReference type="OrthoDB" id="1467120at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028991">
        <property type="entry name" value="KamE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036843">
        <property type="entry name" value="KamE_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16554">
        <property type="entry name" value="OAM_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117778">
        <property type="entry name" value="SSF117778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="1">
        <location>
            <begin position="118"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="261" mass="29107" checksum="66D3E8B9C6098D1D" modified="2009-02-10" version="1">MSGYNLEKKNIDKTLNLKAVKPYGDTMNDGKVQVSFTLPVPYGDEAVEAAKIIMKKMGLENPQVVFSKELTKGFTFFIGYGDCIHTVDYESIHVPKVQVDEMTMYEIDQFIKEKIGRKIVVVGATIGTDAHTVGLDAILNMKGFAGHYGLERYEMFEVYNMGSQVPVEEFVKKAIEVKADALLVSQTVTQKNIHIKHLTELVELLEAEGIRDNVILVVGGPRITHELAKELGYDAGFGPNTFAEHVGAFVAKELYRRMQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFR1</accession>
    <name>B7IFR1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type transport systems, involved in lipoprotein release, ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_432</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104192.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_432"/>
    <dbReference type="EnsemblBacteria" id="ACJ74925">
        <property type="protein sequence ID" value="ACJ74925"/>
        <property type="gene ID" value="THA_432"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_432"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_0"/>
    <dbReference type="OMA" id="ATMLKPN"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="8"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="227" mass="25368" checksum="26593CBE3813C5F7" modified="2009-02-10" version="1">MEIVFEMNQVKKIYKMGDVDVKALDGISFKIKKGEFVIIMGPSGSGKSTTLHIMGCLDKPTSGNVYIDGVDVSKLNDKSLAKIRGEKIGFVFQQFNLLPRLTALENVELPMMYKGVPLKKRRKRAKELLELVGLGDRINHKPTQLSGGQMQRVAIARALANEPSYILADEPTGNLDSKSGEDILEIFKRLNKDGMTVVIVTHDPELELLGNHVIFLRDGKIQEERYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFR2</accession>
    <name>B7IFR2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Efflux transporter, RND family, MFP subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_433</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579577.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_433"/>
    <dbReference type="EnsemblBacteria" id="ACJ74926">
        <property type="protein sequence ID" value="ACJ74926"/>
        <property type="gene ID" value="THA_433"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_433"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_14_4_0"/>
    <dbReference type="OMA" id="IYYESHL"/>
    <dbReference type="OrthoDB" id="1532186at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039562">
        <property type="entry name" value="MFP_biotin_lipoyl_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13533">
        <property type="entry name" value="Biotin_lipoyl_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002854695" evidence="1">
        <location>
            <begin position="24"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Biotin_lipoyl_2" evidence="2">
        <location>
            <begin position="61"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="339" mass="37379" checksum="86B612F49EF5C072" modified="2009-02-10" version="1">MKKKAFILSLMILILLISSCGLNSNKDNVALVKSIEYTVKKSDLVESVSATGKIIPKNYMTIYPEVSGKVVEVYVNEGDFVKKGDPILKIDDEDYKISYLNAKLSYEMVKGNGTLEEELKRLQYEKAYDDFVNTVIKAPISGYIVNFSKKVGDLVNQNQALCVILDRSNFEFEGTIDIIDYSKVQVGQKISIEVEGNSEILQGEITYKQIANISSANVTTATIRADINKVKFPENIFIGASCEGEILTINKKDVLKVPTNAVIVKNGKRYVQVKTGEDENNNPISELREIKIGAITEDFVEVVSGLNEGDVVLIQNKSLSKNLSKAGNAAPPPLVRPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFT1</accession>
    <name>B7IFT1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_454</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579593.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_454"/>
    <dbReference type="EnsemblBacteria" id="ACJ74945">
        <property type="protein sequence ID" value="ACJ74945"/>
        <property type="gene ID" value="THA_454"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_454"/>
    <dbReference type="eggNOG" id="COG0392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039146_3_0_0"/>
    <dbReference type="OMA" id="FKRVFFN"/>
    <dbReference type="OrthoDB" id="1201040at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00374">
        <property type="entry name" value="TIGR00374"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
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        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
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            <end position="99"/>
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            <begin position="119"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="335" mass="38616" checksum="3BD6926D54B716A8" modified="2009-02-10" version="1">MTNKDKKMITIGIILSIIISGIVIFFYSFSLNKNILLSIFSKLSFEKILFSTLLMILSFVFDGIKHYIVFKSLNQKISLISSINSCFVTGYFSAVTPFSVGGQPFQIYYLNKKGIKSTYATQVVFLRLFEMILLMFLVDLTYIFILKNNVIGISRSIITLGLILTLLSSTVILFSILFPKIFIKILEVFKKSKFLNKFIKFEKLENWFIELDETIRYILKNHKLLIAIDFVMMFALLIFQSYVFYYSINIFTELNIPFLHFFSIVNIINAVAFLVPTPGASGSFEIIYSHVLSSFSKKPSLIVNGVLYFRFLSYYLVILIGTLVLIKFVKIKENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFT2</accession>
    <name>B7IFT2_THEAB</name>
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        <submittedName>
            <fullName evidence="8">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
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        <name evidence="8" type="ordered locus">THA_455</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_455"/>
    <dbReference type="EnsemblBacteria" id="ACJ74946">
        <property type="protein sequence ID" value="ACJ74946"/>
        <property type="gene ID" value="THA_455"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_455"/>
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        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_352267_0_0_0"/>
    <dbReference type="OMA" id="GFGIDYG"/>
    <dbReference type="OrthoDB" id="149255at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
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        <location>
            <begin position="241"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="746"/>
            <end position="766"/>
        </location>
    </feature>
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        <location>
            <begin position="172"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="773" mass="87408" checksum="2BD96CECDA13D213" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFX2</accession>
    <name>B7IFX2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">RNA polymerase beta'' chain, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_496</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579615.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_496"/>
    <dbReference type="EnsemblBacteria" id="ACJ74986">
        <property type="protein sequence ID" value="ACJ74986"/>
        <property type="gene ID" value="THA_496"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_496"/>
    <dbReference type="HOGENOM" id="CLU_1668567_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="158" mass="19344" checksum="8B351936D74BB9C0" modified="2009-02-10" version="1">MNHDDILNLTFEENEKDLFVESLRYFFEKSLIINPTYFIYYLLIKKNKSKEYILRFFIEIVIKNNIPIKEANLEFLYMIYEYNSIQKINSLIDEYKNEKSETIDYKKYLDRIEKVYDTTLNPISNKIELLYRLHLLPKTKNEYFKEIIMEGVNQYEKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG23</accession>
    <name>B7IG23_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Acyl carrier protein</fullName>
            <shortName evidence="8">ACP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">acpP</name>
        <name evidence="12" type="synonym">acpP1</name>
        <name evidence="12" type="ordered locus">THA_549</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8 10">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 10">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by AcpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.</text>
    </comment>
    <comment type="PTM">
        <text evidence="10">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by acpS.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100598.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_549"/>
    <dbReference type="EnsemblBacteria" id="ACJ75037">
        <property type="protein sequence ID" value="ACJ75037"/>
        <property type="gene ID" value="THA_549"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_549"/>
    <dbReference type="eggNOG" id="COG0236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108696_5_4_0"/>
    <dbReference type="OMA" id="VKMETTF"/>
    <dbReference type="OrthoDB" id="1943389at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="Acyl_carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="PTHR20863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2 8 9" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4 8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="11">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="8 9">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ75037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="76" mass="8485" checksum="6EF2C0FC92EC0FAB" modified="2009-02-10" version="1">MEKIQKIREIIADQLGVDIEEVTDEKSLTEDLGADSLDMVDLVMAFEDEFGVKVEDSDLSKINTVKDVIDLISARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG49</accession>
    <name>B7IG49_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sensor histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_576</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100549.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_576"/>
    <dbReference type="EnsemblBacteria" id="ACJ75063">
        <property type="protein sequence ID" value="ACJ75063"/>
        <property type="gene ID" value="THA_576"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_576"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5000">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_322055_0_0_0"/>
    <dbReference type="OMA" id="AWIGPAN"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="534"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="664"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="275"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="875" mass="101262" checksum="D1845BC686B8974F" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG82</accession>
    <name>B7IG82_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, LacI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579679.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_610"/>
    <dbReference type="EnsemblBacteria" id="ACJ75096">
        <property type="protein sequence ID" value="ACJ75096"/>
        <property type="gene ID" value="THA_610"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_610"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_15_1_0"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peripla_BP_3" evidence="1">
        <location>
            <begin position="2"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="167" mass="19039" checksum="4F2DB4501E43B285" modified="2009-02-10" version="1">MNHLYENGHRKILFIRGPHLSPAALEREKGVLKFLKKHDDVEVFYSEFEGYEPEIGYESVMAHLKKYGKNFTAIFTINDWTAIGALSALYEKGLKVPEDISLVGFDDAPYVKYIVPPLTTIKQPRWEMGQTAAQLLIERIQNKGSNIPKNVILQPQLIVRKSVKKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG96</accession>
    <name>B7IG96_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dihydrofolate reductase</fullName>
            <ecNumber evidence="3">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">folA</name>
        <name evidence="5" type="ordered locus">THA_624</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dihydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104442.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_624"/>
    <dbReference type="EnsemblBacteria" id="ACJ75110">
        <property type="protein sequence ID" value="ACJ75110"/>
        <property type="gene ID" value="THA_624"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_624"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_4_4_0"/>
    <dbReference type="OMA" id="IMVQSIN"/>
    <dbReference type="OrthoDB" id="1920912at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00158"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012259">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069">
        <property type="entry name" value="PTHR48069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHFR" evidence="4">
        <location>
            <begin position="2"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="162" mass="18679" checksum="C83A2C2A6AA32E5C" modified="2009-02-10" version="1">MHLSMIVVTDIFGGFAISEYDKIEWGSKEDKAHFRELTSKIGTVIMGRKTYESIGFPLKDRLNIVLTSKNYKNSENLIFLNESPIQVIRFLEKNKINEAAVIGGKSVFKQFFPYIDKIYITIEPITLENAEKLSFPYQQFKLISTNILNEKGTILLEYSKLQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG99</accession>
    <name>RL1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplA</name>
        <name type="ordered locus">THA_630</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104445.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IG99"/>
    <dbReference type="SMR" id="B7IG99"/>
    <dbReference type="STRING" id="484019.THA_630"/>
    <dbReference type="EnsemblBacteria" id="ACJ75113">
        <property type="protein sequence ID" value="ACJ75113"/>
        <property type="gene ID" value="THA_630"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_630"/>
    <dbReference type="eggNOG" id="COG0081">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062853_0_0_0"/>
    <dbReference type="OMA" id="GWTDVDV"/>
    <dbReference type="OrthoDB" id="1671455at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_B">
        <property type="entry name" value="Ribosomal_L1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005878">
        <property type="entry name" value="Ribosom_L1_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_L1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_L1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_L1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_L1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_L1_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="SSF56808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01169">
        <property type="entry name" value="rplA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_1000141473" description="50S ribosomal protein L1">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <sequence length="232" mass="25518" checksum="F9FBFD9FAB7926F8" modified="2009-02-10" version="1">MPKHSKRYNEVRKLVDRNKDYDLNEAIDLAKKVATAKFDETVELHIKTNIDYRKSDQQIRSTIALPHGTGKEVKVLVFATGEKAEEAKAAGADYVGAEDLAEKIQKENFLDFDVAIATPDMMRVIGKLGKILGPRGLMPNPKAGTVTNDVASAVKEFKKGRMEVRTDKTGNLHIPVGKASFDNEKLKENIKSAYEQILNLKPAGVKGNFIKKVVLSTTMGPGIKVDPATLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGB6</accession>
    <name>B7IGB6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter integral membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_653</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104495.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_653"/>
    <dbReference type="EnsemblBacteria" id="ACJ75130">
        <property type="protein sequence ID" value="ACJ75130"/>
        <property type="gene ID" value="THA_653"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_653"/>
    <dbReference type="eggNOG" id="COG4587">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084465_1_1_0"/>
    <dbReference type="OMA" id="YALWFAR"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010390">
        <property type="entry name" value="DUF990"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06182">
        <property type="entry name" value="ABC2_membrane_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="274" mass="31978" checksum="2D9CCA725A1D764C" modified="2009-02-10" version="1">MNLQNIIKETFRITNIMKESYILNLKKAFSNRIELFTKLFGYPARLFMFYFLWLSVLENRAFPNLEKSYIIGYYTISLFLSQIFPFIRKSREIRQNIFSGEIANYLARNLPFGIVNLMEYVATISIYLLFVTPIAYVLLGIFANVLPNAPTLIMFLILGFLGSILRYSIWYVIGLVSFYTHENIGIITFYLTIENLFSGSLLPLKVFPETFQKILNILPFRLMLYTPVDTLFNNTSFSDFFINFSLQILWLAFVLILAAIIWKEGLKKFSAYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGC5</accession>
    <name>B7IGC5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycerol kinase</fullName>
            <ecNumber evidence="5">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glycerokinase</fullName>
            <shortName evidence="5">GK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">glpK</name>
        <name evidence="9" type="ordered locus">THA_662</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + glycerol = ADP + H(+) + sn-glycerol 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.30"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="5">Inhibited by fructose 1,6-bisphosphate (FBP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_662"/>
    <dbReference type="EnsemblBacteria" id="ACJ75139">
        <property type="protein sequence ID" value="ACJ75139"/>
        <property type="gene ID" value="THA_662"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_662"/>
    <dbReference type="eggNOG" id="COG0554">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_2_3_0"/>
    <dbReference type="OMA" id="FMLMNIG"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00186">
        <property type="entry name" value="Glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="Carb_kinase_FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="Carb_kinase_FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="5 6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FGGY_N" evidence="7">
        <location>
            <begin position="2"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="FGGY_C" evidence="8">
        <location>
            <begin position="254"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="78"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="237"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="403"/>
            <end position="407"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="489" mass="54817" checksum="2A8155DC1219A030" modified="2009-02-10" version="1">MYILALDQGTTSSRAILFNEKGEYVYGLNKEYKQIYPRPGWVEHDPYDILNSQIEVAKKVVETVGAENIAAIGITNQRETTILWDKNTGKPVYNAIVWQCRRTASICDDLKEKGYEKLIKEKTGLVVDAYFSGTKIKWILDNVEGVREKAERGDILFGTVDSWLIWNLTGGKVHVIDYSNASRTMLFNLKTLDWDDEILEILNIPRAILPKPMPSSYVYGYTEIFGGKIPIAGDAGDQQASLFGQTCFEKGMVKNTYETGCFILMNTGDTPYYSNSGLLTTIAWGVDDKVEYALEGSIFVAGAAVQWLRDNLKLIDNASETEDLAKSVPDSGDLYFVPAMVGLGAPYWDMYARGLLIGITRGTTKAHIVRAVLESIAYQTRDVLEVMSKEAEINMGTLRVDGGASNNNFLMQFQADILGVPVERPEVTETTALGAAYLAGLAVGLWKSKEEIAWNLNKRFEPQLSKDEREKLYSRWKEAVKRSMRWSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGC7</accession>
    <name>B7IGC7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_664</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579710.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_664"/>
    <dbReference type="EnsemblBacteria" id="ACJ75141">
        <property type="protein sequence ID" value="ACJ75141"/>
        <property type="gene ID" value="THA_664"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_664"/>
    <dbReference type="HOGENOM" id="CLU_1119725_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="248" mass="27063" checksum="E940CB6490EA9BF1" modified="2009-02-10" version="1">MKKRLFLLLSGLLVIFIVFSGCLQQLPNDNDGTSSTTSVILDGIAKAKYDFLTIGATNLEITGKVLATDGRNVIVHDATSGILIYDSNFANSTGAAVGDLITLNATFVYYYNVIEGKYNTVSYVSTDTSSEELKPVKLDITLDPDWLTDADKEGNLTKTSGDATSLSLWLFRYVTVEGTITLSEGNYAAVEYETKDGTSCIEFTSWNSALNYDDIKANIGTTVAATGILTANFDKWQLLIWDSNNIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGF1</accession>
    <name>B7IGF1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH:ubiquinone oxidoreductase, na translocating, f subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nqrF</name>
        <name evidence="7" type="ordered locus">THA_694</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579733.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_694"/>
    <dbReference type="EnsemblBacteria" id="ACJ75165">
        <property type="protein sequence ID" value="ACJ75165"/>
        <property type="gene ID" value="THA_694"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_694"/>
    <dbReference type="eggNOG" id="COG2871">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_7_2_0"/>
    <dbReference type="OMA" id="FIKEFVV"/>
    <dbReference type="OrthoDB" id="1834577at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="5">
        <location>
            <begin position="32"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="6">
        <location>
            <begin position="128"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="367" mass="41119" checksum="F19FC14AC43166C7" modified="2009-02-10" version="1">MNIWLAPLIVGIVSSVLSALIVIVDSIVNNYGEVEIDINNGKKKLKVKGGSPLLFTLASENIFVPSACGGRGSCGACKVKVLSDVGEYLPTELPYMSEEETKENIRLSCQIKVKKDIKIQLPEELFNVKKLTGKVVSIKNVTHDIKEVRIKLPEEINFKAGQYVQIVVPPYDKIKQPTQRAYSIASTPSKKDEIDLLIRLVPGGIATTYVHNYLKEGDNLEVIGPFGEFYMRDTDADMICVAGGSGMAPIKSIVLDMYERGITNRNVWYFFGARTEKDLFYVELFKDLEKKWSNFHFIPALSEPIEPEKWNGEVGLITDVMVKYLENVVDKNTKKEGYLCGSPGMINACEKLLNEHGIKDVYYDKFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGG2</accession>
    <name>B7IGG2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Retinol dehydrogenase 13, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_705</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579741.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_705"/>
    <dbReference type="EnsemblBacteria" id="ACJ75176">
        <property type="protein sequence ID" value="ACJ75176"/>
        <property type="gene ID" value="THA_705"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_705"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_5_0"/>
    <dbReference type="OMA" id="SDCKKTW"/>
    <dbReference type="OrthoDB" id="1377545at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="331" mass="38432" checksum="0F20F744B7CDF43A" modified="2009-02-10" version="1">MNKIRQYWKEYKWSNVFQMIRNMKQEVKKCEKRADGKFVVITGATSGIGYHAAMKFASMGANLLTINRNKEKSERLKEEILNKYPVEFDYYISDLSILEDIYRVGEFLNKLDKDIDILIHNAGVYNTKKRITKDGFEETFVVHFIAPFIINNMLLEKFNRQGKGRIIFVSSEGYRFAVWGPNLDDLMFERTKYSGLKAYGSAKICQILTMHIYAKLLKGPTIIAMHPGMVRSNTGRDNGKLYKIFKKTLDKLSDSPEISAEALYYLGVSEELENVTDKFYHLTVEEELTPPARDMEVAVELWKKTLELVKFCEVVTCKNEKLLLLEEEFQV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-10-13" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGK8</accession>
    <name>OBG_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTPase Obg</fullName>
            <ecNumber evidence="1">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP-binding protein Obg</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">obg</name>
        <name type="ordered locus">THA_757</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100287.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IGK8"/>
    <dbReference type="SMR" id="B7IGK8"/>
    <dbReference type="STRING" id="484019.THA_757"/>
    <dbReference type="PRIDE" id="B7IGK8"/>
    <dbReference type="EnsemblBacteria" id="ACJ75222">
        <property type="protein sequence ID" value="ACJ75222"/>
        <property type="gene ID" value="THA_757"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_757"/>
    <dbReference type="eggNOG" id="COG0536">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011747_2_1_0"/>
    <dbReference type="OMA" id="VVFDWEP"/>
    <dbReference type="OrthoDB" id="603226at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01454">
        <property type="entry name" value="GTPase_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014100">
        <property type="entry name" value="GTP-bd_Obg/CgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036346">
        <property type="entry name" value="GTP-bd_prot_GTP1/OBG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015349">
        <property type="entry name" value="OCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="PTHR11702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09269">
        <property type="entry name" value="DUF1967"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102741">
        <property type="entry name" value="SSF102741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="SSF82051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02729">
        <property type="entry name" value="Obg_CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03595">
        <property type="entry name" value="Obg_CgtA_exten"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51881">
        <property type="entry name" value="OCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000386352" description="GTPase Obg">
        <location>
            <begin position="1"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Obg" evidence="3">
        <location>
            <begin position="4"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="1">
        <location>
            <begin position="163"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="OCT" evidence="2">
        <location>
            <begin position="355"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="169"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="194"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="215"/>
            <end position="218"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="285"/>
            <end position="288"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="314"/>
            <end position="316"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01231"/>
        </source>
    </evidence>
    <sequence length="434" mass="48162" checksum="8D3F189D72E73747" modified="2009-02-10" version="1">MQKADFIDRIVIYVKGGKGGDGSASFRHEKYVPKGGPDGGDGGNGGYVFLKANPNLSTLLSVSEKKKYIAENGENGKGKKMHGRNGKDVVIDVPVGTVVKDFETGEIIADLDKPGMVVCVARGGKGGRGNVHFKSSTMRAPKISERGFEGEERKLVLELKLLADVGLVGYPNVGKSSFISKISNARPKIANYPFTTTIPNLGVVTVNELQFVVADIPGLIKGASKGAGLGNVFLRHVERCSVIAHIVDISGMEGRDPVQDYFDIRNELEHFSSELAQKEEIIIANKIDLISKEELEKRLEKLRKETGKQIFPTSIITGEGIEKIVYKLAEIVKESKKFHTQSEVVNEQIKRPKPLWKELPERFNIEIIKEGNDFIVTGTYVEEWAKRLNLNQKDGYRKFMELLEKNGLEKKLKEAGIKDGDTVWVAQRAFEYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGM0</accession>
    <name>B7IGM0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type dipeptide/oligopeptide/nickel transport systems, permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_771</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579788.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_771"/>
    <dbReference type="EnsemblBacteria" id="ACJ75234">
        <property type="protein sequence ID" value="ACJ75234"/>
        <property type="gene ID" value="THA_771"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_771"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_8_0_0"/>
    <dbReference type="OMA" id="QDPTTYH"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="74"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="280" mass="31249" checksum="AE46BEA34A85DEED" modified="2009-02-10" version="1">MREIMFFAFRNKKLNIGLSIVLFFLLLAIFGPYISKYKDPLEYVGMGYQPPSKEFWLGTTTFGQDVFTQLVFGLRSSFFVGLIGGGLATFVGLIVGFFAGYEGGIIDEILMMLTNILLVIPTLALLIIVASYLPYRGVFIQSVIIGLTAWPWTARAVRSQTLSLKMREFVNLARITGRSHLKIIIYEILPNMLSYVFMVFILQFGGAILAAVGLDFIGLGPTQGISLGLMMQNAVLWNAIQLGMWWWAIPPGLVITLIVGALYFMNTGLDEVFNPKLREM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGN6</accession>
    <name>B7IGN6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Redox-sensing transcriptional repressor Rex</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rex</name>
        <name evidence="8" type="ordered locus">THA_788</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Modulates transcription in response to changes in cellular NADH/NAD(+) redox state.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the transcriptional regulatory Rex family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579796.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_788"/>
    <dbReference type="EnsemblBacteria" id="ACJ75250">
        <property type="protein sequence ID" value="ACJ75250"/>
        <property type="gene ID" value="THA_788"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_788"/>
    <dbReference type="eggNOG" id="COG2344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061534_1_0_0"/>
    <dbReference type="OMA" id="GKPKVGY"/>
    <dbReference type="OrthoDB" id="1872374at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051775">
        <property type="term" value="P:response to redox state"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01131">
        <property type="entry name" value="Rex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009718">
        <property type="entry name" value="Rex_DNA-bd_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022876">
        <property type="entry name" value="Tscrpt_rep_Rex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35786">
        <property type="entry name" value="PTHR35786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06971">
        <property type="entry name" value="Put_DNA-bind_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CoA_binding" evidence="7">
        <location>
            <begin position="83"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="94"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="214" mass="23764" checksum="7094FBC525D6B881" modified="2009-02-10" version="1">MSNLKKDRKPLEIPKPTFERLKKYLALLLQEESEYISSEGIAEKLNITPEQVRKDFTYINIKGKPKVGYHIPPLINELSELFGIGVMDNIIIVGAGNLGSALAKYAGFKKIGVRVVAIFDNDPVKIGSFVGELSVLPFSEENLKRVIKRFKVKIGVICVTEESAQEVANVLVRNGIKALWNFAPVTLKVPPGIVLENQDITTGVLTIKHLLEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGN8</accession>
    <name>B7IGN8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UDP-N-acetylmuramyl pentapeptide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_790</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579798.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_790"/>
    <dbReference type="EnsemblBacteria" id="ACJ75252">
        <property type="protein sequence ID" value="ACJ75252"/>
        <property type="gene ID" value="THA_790"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_790"/>
    <dbReference type="eggNOG" id="COG0770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035297_0_0_0"/>
    <dbReference type="OMA" id="LQWYHYS"/>
    <dbReference type="OrthoDB" id="1861122at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="3">
        <location>
            <begin position="172"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="2">
        <location>
            <begin position="384"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="514" mass="60413" checksum="155C73F23DD3E449" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGP6</accession>
    <name>B7IGP6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_798</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579803.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_798"/>
    <dbReference type="EnsemblBacteria" id="ACJ75260">
        <property type="protein sequence ID" value="ACJ75260"/>
        <property type="gene ID" value="THA_798"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_798"/>
    <dbReference type="eggNOG" id="ENOG502ZQ0Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1479949_0_0_0"/>
    <dbReference type="OMA" id="FVFYNAL"/>
    <dbReference type="OrthoDB" id="1429565at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="185" mass="21502" checksum="081A8839C8B387CE" modified="2009-02-10" version="1">MEIKVSNLELFSIPFGISVLWNLIFQAKINLLDKLSKQYENAHFVFSNILGDFGVYDKVNEILSAYFKNYSITDEEFDFNSSPINNIPIIQIDNEKQKFETETIGIFYPSDLSNLPEKKYSKNIIVLKQVVDQQTYEKLAEDYIVIGDFEKLEYKNLLERKVTDLTQDDATKSFLLKVLRGDKVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGS1</accession>
    <name>B7IGS1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_823</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_823"/>
    <dbReference type="EnsemblBacteria" id="ACJ75285">
        <property type="protein sequence ID" value="ACJ75285"/>
        <property type="gene ID" value="THA_823"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_823"/>
    <dbReference type="HOGENOM" id="CLU_3206349_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="45" mass="5316" checksum="EEC3A126EB45413D" modified="2009-02-10" version="1">MVPYILTRSEIFESKMIGIFLADNKVFFLNFQLSQFGLIHHIQDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGS3</accession>
    <name>B7IGS3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alkaline phosphatase 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_825</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100426.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_825"/>
    <dbReference type="EnsemblBacteria" id="ACJ75287">
        <property type="protein sequence ID" value="ACJ75287"/>
        <property type="gene ID" value="THA_825"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_825"/>
    <dbReference type="eggNOG" id="COG1785">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008539_6_2_0"/>
    <dbReference type="OMA" id="GYNTTPV"/>
    <dbReference type="OrthoDB" id="585847at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="PTHR11596"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857409" evidence="7">
        <location>
            <begin position="19"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="436" mass="47914" checksum="C807B266B444A3DE" modified="2009-02-10" version="1">MKKLLLCLFLLLALFSFAVTNVIYMVGDGMGINHALLASYLEGRILNFLKTQNIGIITTHSANSNVTDSAAAGTALFTGFKTNNGMIGMLPDGTIVPTIAEIAAKQGVKIGIISTSRITHATPGAVFGHVESRDDENTLAEQLVNSPITVAFGGGWRHFVATGGKRKDGKDLIKLAKEKGFDYITTKEELLNYKGNKVIGLFNSSHLPAASDRIKEPMLAEMTKKALEILSKDGKPFFLMIEGSQIDWEAHGNDPFGVWKEIIEFEQAFNVAMEFLKENPDTLIIVTADHETGGLGLSTGGYFFDVEMLRQFTKNTDFIAKQANNDKNKVRELIKQYYGIELTDEDMEYFDLIVKEQAGSYYAFSNAIGRVVSRKAHLGWTTFAHTGAPVALYAFGKGAENFNGFYDNTEIPRIIGRIAGYELTFPTYELPVSGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGS8</accession>
    <name>B7IGS8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Membrane spanning protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_830</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100443.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_830"/>
    <dbReference type="EnsemblBacteria" id="ACJ75292">
        <property type="protein sequence ID" value="ACJ75292"/>
        <property type="gene ID" value="THA_830"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_830"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1081556_0_0_0"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="4">
        <location>
            <begin position="3"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Fe-ADH" evidence="3">
        <location>
            <begin position="159"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="257" mass="29257" checksum="B42AABE92451AF69" modified="2009-02-10" version="1">MQYISLILVIIVWGLSFIATSIIVQNISPLLAAFIRFSIALLTLLVIPKNRKINLFNIHKVLAGFWGITIYFVSENFALKFTTPTNAALIVSTAPIWYVLFTQIAHKRKTHSLQYVGSLIALFGVGLVILNGRIILKLNPIGDLLAFFAAISWVLYTHHNLFEHIFDYIFERLAIVGREVFGVYESDDKKAARLAIKKLRDFQEQFNLNKTLKELNVRKEDLEEIAKTGYRVLKGVVVVTPGNIDDKDMLKILQNCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGV7</accession>
    <name>B7IGV7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75321">
        <property type="protein sequence ID" value="ACJ75321"/>
        <property type="gene ID" value="THA_861"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_861"/>
    <dbReference type="HOGENOM" id="CLU_2482252_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="87" mass="10691" checksum="8F21BEEA5A5B943D" modified="2009-02-10" version="1">MLKQFYLNFNSCKVRYKPPPNLTFLFKFHKFQFLQGKVQTISLTYAVKKHCINFNSCKVRYKLIILNTIRYRNKNFNSCKVRYKRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGZ4</accession>
    <name>B7IGZ4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP-N-acetylmuramyl-tripeptide synthetase</fullName>
            <ecNumber evidence="7">6.3.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">UDP-MurNAc-tripeptide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 12" type="primary">murE</name>
        <name evidence="12" type="ordered locus">THA_899</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">Carboxylation is probably crucial for Mg(2+) binding and, consequently, for the gamma-phosphate positioning of ATP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the MurCDEF family. MurE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.-" evidence="7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579870.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_899"/>
    <dbReference type="EnsemblBacteria" id="ACJ75358">
        <property type="protein sequence ID" value="ACJ75358"/>
        <property type="gene ID" value="THA_899"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_899"/>
    <dbReference type="eggNOG" id="COG0769">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022291_4_1_0"/>
    <dbReference type="OMA" id="FIPGRML"/>
    <dbReference type="OrthoDB" id="1861122at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00208">
        <property type="entry name" value="MurE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005761">
        <property type="entry name" value="UDP-N-AcMur-Glu-dNH2Pim_ligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="SSF63418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01085">
        <property type="entry name" value="murE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 7 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 7 8" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7 8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="9">
        <location>
            <begin position="27"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="11">
        <location>
            <begin position="107"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="10">
        <location>
            <begin position="326"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-MurNAc-L-Ala-D-Glu binding" evidence="7">
        <location>
            <begin position="151"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="109"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="7">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ75358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="493" mass="55392" checksum="86BC3900490641A0" modified="2009-02-10" version="1">MKIKDILRELNSLILKVNCDEELEFNYISSNSKDIQENTLFICRKGTSFDSHNIVDDLYKEGANVFVVEREIRKYPYIQVVDSRIAESIIASMFYSKPYEKLITFGVTGTNGKTTSVHLFHHIMHNFGIKGSISGTVVNDIMGDKFYHPNTTPSAIEIMRNMHKTLENGGKYYGMEVSSHALSQYRVESIRYDIVGITNITRDHLDFHETFENYKKAKFHILNLLKPNGIAVVNHELLQEVKNKNKNIKLVSFGVDSSSDYVIHEITTNWSGTFFKLDTPYGEKKVASQLIGEYNAFNITLVVAALAEIGYDIDEVIASVATFRGVDGRFEIIPEAKKLGIEIVVDFAHTPDALEKVILSLRKLTKGRLITVFGAGGQSDIGKRPMMGEVVSKYSDISIITTDDPRGEDPLKIIKDVEAGLVPGSLSLSIEDRKEAIDTAITLANRGDVILIAGRGHEPYQVFDENHKVPFKDRDVAIDIIFQKINKGEKTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH01</accession>
    <name>B7IH01_THEAB</name>
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        <recommendedName>
            <fullName evidence="1">AAA-ATPase_like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579875.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_906"/>
    <dbReference type="EnsemblBacteria" id="ACJ75365">
        <property type="protein sequence ID" value="ACJ75365"/>
        <property type="gene ID" value="THA_906"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_906"/>
    <dbReference type="eggNOG" id="COG1672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021114_0_1_0"/>
    <dbReference type="OMA" id="YLIEMLR"/>
    <dbReference type="OrthoDB" id="191489at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018631">
        <property type="entry name" value="AAA-ATPase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012547">
        <property type="entry name" value="PDDEXK_9"/>
    </dbReference>
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        <property type="entry name" value="AAA-ATPase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08011">
        <property type="entry name" value="PDDEXK_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA-ATPase_like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="520" mass="60735" checksum="9A2A1095B0711125" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH04</accession>
    <name>B7IH04_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Flagellar L-ring protein FlgH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">flgH</name>
        <name evidence="7" type="ordered locus">THA_909</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Assembles around the rod to form the L-ring and probably protects the motor/basal body from shearing forces during rotation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FlgH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100779.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_909"/>
    <dbReference type="EnsemblBacteria" id="ACJ75368">
        <property type="protein sequence ID" value="ACJ75368"/>
        <property type="gene ID" value="THA_909"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_909"/>
    <dbReference type="eggNOG" id="COG2063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069313_2_0_0"/>
    <dbReference type="OMA" id="QIWENGK"/>
    <dbReference type="OrthoDB" id="1900876at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009427">
        <property type="term" value="C:bacterial-type flagellum basal body, distal rod, L ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000527">
        <property type="entry name" value="Flag_Lring"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34933">
        <property type="entry name" value="PTHR34933"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02107">
        <property type="entry name" value="FlgH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01008">
        <property type="entry name" value="FLGLRINGFLGH"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857376" evidence="6">
        <location>
            <begin position="19"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="195" mass="21409" checksum="0469DDEF73E54351" modified="2009-02-10" version="1">MKKLLTVFLILFSLLSLADSIYNPNSKFSNIIASPRASKVGDVVNILVYESPRFSTSSEVDSFKNTILGAISSGSKILGSDLSGFLPIKDSDKVKSSNKSQTSVILQITAVVKKVDEYGNLYVEGRKNIKVGNDLREIIITGWVNPEMISAKNTVNSTDLMDAQIWENGKIIFEDDPNEGSWLGLILSSLAELFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH09</accession>
    <name>B7IH09_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_914</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100792.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_914"/>
    <dbReference type="EnsemblBacteria" id="ACJ75373">
        <property type="protein sequence ID" value="ACJ75373"/>
        <property type="gene ID" value="THA_914"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_914"/>
    <dbReference type="eggNOG" id="COG0621">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_1_0_0"/>
    <dbReference type="OMA" id="HFHIPLQ"/>
    <dbReference type="OrthoDB" id="397139at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035596">
        <property type="term" value="F:methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006467">
        <property type="entry name" value="MiaB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01579">
        <property type="entry name" value="MiaB-like-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="9">
        <location>
            <begin position="133"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="429" mass="49124" checksum="751C0F627A36B17A" modified="2009-02-10" version="1">MRVSVLTYGCKLNQYESELMAEKLENEGYIVVNEEVESDVFVINSCVVTNEATRKIKQQIRRLKRKFPGAKVVVTGCYSQLGFEELEKEGVDLVLGNNEKKYIDRYLGESGIFVDKAYWNRNSLEDEFVFSSLAERTRAFIKVQDGCTNTCSYCAIRFARGNKIRSKPVDLVVSEVLRLVNKDYKEIVITGLNLGKFGKDIDSSLHELLRSLVKIKGDFRIRLSSINPEDLDEELISLIGAEEKICNHLHIPLQSGSTDVLKNMRRNYTQDDYLRVVDSIRKVDPNFSITTDIMVGFPGESEKDFEETLKVVREVLFSKVHAFRYSDRPNTLASKMPKKVPGNVKKERVEVLEKLSAAVAKDYRKRLVGRKAKVLIESYKNKIYSGYDEYYVLHETSHGEFGKIENVTIQAVTDEGVISKKYERKVSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH30</accession>
    <name>B7IH30_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycerol-3-phosphate acyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Acyl-PO4 G3P acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Acyl-phosphate--glycerol-3-phosphate acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">G3P acyltransferase</fullName>
            <shortName evidence="10">GPAT</shortName>
            <ecNumber evidence="10">2.3.1.275</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Lysophosphatidic acid synthase</fullName>
            <shortName evidence="10">LPA synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">plsY</name>
        <name evidence="11" type="ordered locus">THA_935</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl phosphate + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:34075"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="2.3.1.275"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Probably interacts with PlsX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the PlsY family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.275" evidence="10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100835.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_935"/>
    <dbReference type="EnsemblBacteria" id="ACJ75394">
        <property type="protein sequence ID" value="ACJ75394"/>
        <property type="gene ID" value="THA_935"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_935"/>
    <dbReference type="eggNOG" id="COG0344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081254_4_0_0"/>
    <dbReference type="OMA" id="WRHRGNL"/>
    <dbReference type="OrthoDB" id="1691856at2"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043772">
        <property type="term" value="F:acyl-phosphate glycerol-3-phosphate acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01043">
        <property type="entry name" value="PlsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003811">
        <property type="entry name" value="G3P_acylTferase_PlsY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30309">
        <property type="entry name" value="PTHR30309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02660">
        <property type="entry name" value="G3P_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01207">
        <property type="entry name" value="G3P_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00023">
        <property type="entry name" value="TIGR00023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="138"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="201" mass="22102" checksum="21634B8A862969D2" modified="2009-02-10" version="1">MEHHIYAILIGYFLGAIPFSFLIGKLKGIDIRKVGSGNVGGTNVLRSAGALYGALAFFLDILKGYLAVIFVSSFGINAMLLSGSFAVFGHCFPIYLKFKGGKGVATTFGVFLAINPWSGLVFFLIWLLIVIFTKYVSLGSIVGLLGASIFTFFIGKDYWVIFLALALFSTLRHKENIKRLLNGNERKTDVVKYFFGKGKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH48</accession>
    <name>B7IH48_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Indolepyruvate oxidoreductase subunit IorA</fullName>
            <shortName evidence="14">IOR</shortName>
            <ecNumber evidence="3 14">1.2.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Indolepyruvate ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">iorA</name>
        <name evidence="17" type="ordered locus">THA_953</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>CoA + indole-3-pyruvate + 2 oxidized [2Fe-2S]-[ferredoxin] = (indol-3-yl)acetyl-CoA + CO2 + H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:12645"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57271"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="EC" id="1.2.7.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="14 15">Binds 2 [4Fe-4S] clusters. In this family the first cluster has a non-standard and varying [4Fe-4S] binding motif CX(2)CX(2)CX(4-5)CP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of the IorA and IorB subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.8" evidence="3 14"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579898.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_953"/>
    <dbReference type="EnsemblBacteria" id="ACJ75412">
        <property type="protein sequence ID" value="ACJ75412"/>
        <property type="gene ID" value="THA_953"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_953"/>
    <dbReference type="eggNOG" id="COG4231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017727_0_0_0"/>
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        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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    </dbReference>
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    <keyword evidence="8 14" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
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    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="16">
        <location>
            <begin position="580"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="16">
        <location>
            <begin position="611"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011238"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012812"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00017710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033657"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR006439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR006439-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ACJ75412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="642" mass="70262" checksum="47B28E553BB1109C" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH90</accession>
    <name>B7IH90_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, LacI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_995</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100989.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_995"/>
    <dbReference type="EnsemblBacteria" id="ACJ75454">
        <property type="protein sequence ID" value="ACJ75454"/>
        <property type="gene ID" value="THA_995"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_995"/>
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    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
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        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
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        <property type="entry name" value="SBP_2_dom"/>
    </dbReference>
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        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peripla_BP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00036">
        <property type="entry name" value="HTHLACI"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="327" mass="37447" checksum="04DC4C55426F775C" modified="2009-02-10" version="1">MKKRSVTIKDVARKAGVGVGTVSRVINNSPHVNQRTKEHVLKVIQELGYMPNPHARRLSSGHTKIITTIFPQMVGEFHQLLLSGIDEIFEKEGYTSFVYPLYSENRYKFVRESSDFVLGTDGVIIDALNVDRLLQQYIPKNVPVVSVEFDSEKYDSVIIDNFYGGMIAGDYLSNFEGDIFVITHRRKSKLESTVFEERVSGFIESVEKKGRTIERIFEIELDWLEAFNVAKEIFTRSNKVIIFTTTDYFAFPVIEFARIKGFVPKKDFHLCGFDNLTLSNILNITTIKQPIVEMGKSAGELLLRRIKGFTRKKQTVAFKPEIIIRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHF4</accession>
    <name>B7IHF4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1062</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579969.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1062"/>
    <dbReference type="EnsemblBacteria" id="ACJ75518">
        <property type="protein sequence ID" value="ACJ75518"/>
        <property type="gene ID" value="THA_1062"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1062"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_5_0_0"/>
    <dbReference type="OMA" id="FSTMVAY"/>
    <dbReference type="OrthoDB" id="1074175at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="190"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="394" mass="45195" checksum="7D12C8B061F02BDD" modified="2009-02-10" version="1">MKKAILLTIMFILLIISLFPMFSMFYTAVIPSGNLTKVIKERYINDFEIKYMSYLKRKVKSEGKISIVNESPQSTRSILASGPIELLVDKLDLRVAKYIEFWVKGEGDFSVEIRDAFSKSVSKNFKATGEWKKYIIDYKDLKEINLKYVSKLVFNFDGQYYLDDVKVKYKFPTLLNFINVLKEDMFSRYILNSLIVSTIVVIGNIIFSTMVAYAFARRKFFGKNVLFSIVLATMMIPPQVTIIPIFIIMKNIGWIDTYFALTVPMLVTPFSIFLLKQYIEQLPIELEQAAYVDGANTFQILFKIIFPLSKPAIAVMAINTFIATWNDLFYPLVMTNSREMRTVQVGLALYQKLNQIDWPRLMAASSIIGLPVIIIFLAFQKHIISGITKGALKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHG4</accession>
    <name>B7IHG4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Precorrin-4 C11-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cobM</name>
        <name evidence="7" type="ordered locus">THA_1072</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579978.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1072"/>
    <dbReference type="EnsemblBacteria" id="ACJ75528">
        <property type="protein sequence ID" value="ACJ75528"/>
        <property type="gene ID" value="THA_1072"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1072"/>
    <dbReference type="eggNOG" id="COG2875">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_7_1_0"/>
    <dbReference type="OMA" id="WSAMGEQ"/>
    <dbReference type="OrthoDB" id="1185397at2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046026">
        <property type="term" value="F:precorrin-4 C11-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11641">
        <property type="entry name" value="Precorrin-4_C11-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006362">
        <property type="entry name" value="Cbl_synth_CobM/CibF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01465">
        <property type="entry name" value="cobM_cbiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="252" mass="28502" checksum="C5E5922291A6758E" modified="2009-02-10" version="1">MVYFIGAGPGDPELLTLKGYKILKSCQVVIYAGSLVNPEILNFADKNAKVYDSSKMTLEEILEVIRRYKDKDIARLHTGDPSLFGAINEQIELLEKEGIPYKIIPGVSSIFAGAASLKRELTVPSITQTVIITRYGKRTYVPEELEKLSKHNATLVLFLSVKYLKEIVDILKRNYPSNTPISIVYKASWKDENVVSGTLEDIVEKAGNIESTALIYVGRFLEVIKTRSKLYDKTFSHMYRRSEYEDSNSFDN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHI2</accession>
    <name>GUAA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GMP synthase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="1">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GMP synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">guaA</name>
        <name type="ordered locus">THA_1090</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579994.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHI2"/>
    <dbReference type="SMR" id="B7IHI2"/>
    <dbReference type="STRING" id="484019.THA_1090"/>
    <dbReference type="MEROPS" id="C26.957"/>
    <dbReference type="PRIDE" id="B7IHI2"/>
    <dbReference type="EnsemblBacteria" id="ACJ75546">
        <property type="protein sequence ID" value="ACJ75546"/>
        <property type="gene ID" value="THA_1090"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1090"/>
    <dbReference type="eggNOG" id="COG0518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0519">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_0_5_0"/>
    <dbReference type="OMA" id="KRKIIGH"/>
    <dbReference type="OrthoDB" id="504464at2"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00344">
        <property type="entry name" value="GMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022955">
        <property type="entry name" value="GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0332">GMP biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000120444" description="GMP synthase [glutamine-hydrolyzing]">
        <location>
            <begin position="1"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="3"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="GMPS ATP-PPase" evidence="1">
        <location>
            <begin position="193"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="220"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00344"/>
        </source>
    </evidence>
    <sequence length="513" mass="57995" checksum="E0FE2E2A037CA137" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHJ1</accession>
    <name>B7IHJ1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bifunctional aspartokinase/homoserine dehydrogen ase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580001.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1099"/>
    <dbReference type="EnsemblBacteria" id="ACJ75555">
        <property type="protein sequence ID" value="ACJ75555"/>
        <property type="gene ID" value="THA_1099"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1099"/>
    <dbReference type="eggNOG" id="COG0527">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_6_3_0"/>
    <dbReference type="OMA" id="ISHGERC"/>
    <dbReference type="OrthoDB" id="1067792at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="383" mass="43518" checksum="EEC62608BD05E091" modified="2009-02-10" version="1">MKVIKLGGSIFKEKNSFLKVPPLLDQDNLVIVVSAPYRVTDLLLNNQINKKLLENIYQKLAGQMGFESEKLKERVYEILNEKDYEKIISHGERCNSLALKLFLEKEGFNVEEKLPEEIELNLTKNGEKIELKRSIKSYFKEKKIYIVPGFYAIHNGKIKTLGRGGSDYTATALANILDAENVTLYKDVSQLFTCDPKLVKNPFPITKISYEIAELLSYFGAKIIQYKAIAPAKSKNIPIIIKGLDKSGSTIIGNETNVGVLSISFLKDLLFINGKLKKIPKNVKYIDIFQNNANVIIEKEKFESIKDNFSTITIKENLSLILVGGDLDIGKKLLILNSLYFHKFNFWLLKDAKNFLILLVENKNLKNILNVIHNILFAREGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHK6</accession>
    <name>B7IHK6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580010.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1117"/>
    <dbReference type="EnsemblBacteria" id="ACJ75570">
        <property type="protein sequence ID" value="ACJ75570"/>
        <property type="gene ID" value="THA_1117"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1117"/>
    <dbReference type="HOGENOM" id="CLU_2221968_0_0_0"/>
    <dbReference type="OMA" id="DLFWITI"/>
    <dbReference type="OrthoDB" id="2214412at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="106" mass="12576" checksum="777BF0D9F3974253" modified="2009-02-10" version="1">MSYIEAVIAIVLVFVAVTAVFTSIEYQQKMYSKILERELLQLYSEDLFWITIYNLPNTTPFDSFETSYSTKNAYVVKDYEKIEKEINCITFTFPSNKKVNIYYFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHL2</accession>
    <name>B7IHL2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fructose-1,6-bisphosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">glpX</name>
        <name evidence="6" type="ordered locus">THA_1123</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the FBPase class 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101226.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1123"/>
    <dbReference type="EnsemblBacteria" id="ACJ75576">
        <property type="protein sequence ID" value="ACJ75576"/>
        <property type="gene ID" value="THA_1123"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1123"/>
    <dbReference type="eggNOG" id="COG1494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054938_0_0_0"/>
    <dbReference type="OMA" id="AVFYMDK"/>
    <dbReference type="OrthoDB" id="879338at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01516">
        <property type="entry name" value="FBPase_glpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004464">
        <property type="entry name" value="FBPase_class-2/SBPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30447">
        <property type="entry name" value="PTHR30447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03320">
        <property type="entry name" value="FBPase_glpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004532">
        <property type="entry name" value="GlpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00330">
        <property type="entry name" value="glpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="93"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="169"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="191"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR004532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR004532-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR004532-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="339" mass="37115" checksum="F9FB7842630325EA" modified="2009-02-10" version="1">MSQLSKEITLELVRVTEAAALMASKYLGRGDKEMVDKLASDAMRGMLDYIDMQGVVIIGEGEKDKAPMLYIGEQVGNWEPNTPELDIAVDPIDGTRLVAYGLPNAISVIAATDRGAIEYLPTFYSYKLAVGPELAGKLDINASIRENLRVAAAILNIDISELTVVILNRDRHREIIEEVRKVGARIKLISDGDIAAAIATALPESYVDIYIGIGGSPEATLAAAALKSLGGEIQIKLWPIDEKERNDLIEKGYDLEKVYKTDDLIKSDNVIFAATGVTDGDLLRGVKYSKNIAITESLVMRSKTKTLRKIISHHNLKYKTIPLKSEGEVRFINGKKGND</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHN9</accession>
    <name>B7IHN9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580039.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1151"/>
    <dbReference type="EnsemblBacteria" id="ACJ75603">
        <property type="protein sequence ID" value="ACJ75603"/>
        <property type="gene ID" value="THA_1151"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1151"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059362_3_0_0"/>
    <dbReference type="OMA" id="CAIAAYD"/>
    <dbReference type="OrthoDB" id="1847197at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020051">
        <property type="entry name" value="SagB-type_dehydrogenase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03605">
        <property type="entry name" value="antibiot_sagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="52"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="236" mass="26810" checksum="AF580150741D1A26" modified="2009-02-10" version="1">MKSREFLKSNWRQIKNTDREKGIEKPEIEKDCEKVIKLPEFNLGNKPLREVIEERKSIRKYTDTPMSLQELSFLLWATQGVRKYIQDKKVVFRTVPSAGATHPFDTYLVVFNVDGLEKGIYRYVSLKHGLCKVKPGDFKDKIIDATLGQQFVGNSAVVFVWVAVPYRTEWKYTAESYKAIAIDVGHVCQNLYLAATSIDCGTCAVAAYDQEKMDNLIGVDGDNEMVIYLAPVGKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHQ9</accession>
    <name>B7IHQ9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Outer membrane protein, OMP85 family, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1172</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580056.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1172"/>
    <dbReference type="EnsemblBacteria" id="ACJ75623">
        <property type="protein sequence ID" value="ACJ75623"/>
        <property type="gene ID" value="THA_1172"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1172"/>
    <dbReference type="eggNOG" id="COG4775">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_369531_0_0_0"/>
    <dbReference type="OMA" id="GNTIFTD"/>
    <dbReference type="OrthoDB" id="258122at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010827">
        <property type="entry name" value="BamA/TamA_POTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034746">
        <property type="entry name" value="POTRA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="PTHR12815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07244">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51779">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="POTRA" evidence="3">
        <location>
            <begin position="19"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="3">
        <location>
            <begin position="91"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="722" mass="82506" checksum="D96BBC9246966FE3" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHR5</accession>
    <name>B7IHR5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cell division protein, RodA/ftsW/spoVE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1179</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101382.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="SMR" id="B7IHR5"/>
    <dbReference type="STRING" id="484019.THA_1179"/>
    <dbReference type="EnsemblBacteria" id="ACJ75629">
        <property type="protein sequence ID" value="ACJ75629"/>
        <property type="gene ID" value="THA_1179"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1179"/>
    <dbReference type="eggNOG" id="COG0772">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029243_2_1_0"/>
    <dbReference type="OMA" id="KLWWSNL"/>
    <dbReference type="OrthoDB" id="1133883at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018365">
        <property type="entry name" value="Cell_cycle_FtsW-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001182">
        <property type="entry name" value="FtsW/RodA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474">
        <property type="entry name" value="PTHR30474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01098">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00428">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="363" mass="41181" checksum="5680E9B29313F091" modified="2009-02-10" version="1">MRYSIMLLVTAIFVLFIIASVALYSIDFAREYYSTGSNSNFFFKYIVYISLSMILALLIIFFLPERFIEDKKYVWFFYIIVLAMLVLPLYGPFSSYKNGARRWIEVSGNTFQPSELAKIFVIYFIAHYIKDNKEKLSSFWDGLIKPLLLISPILLLIFVEPDLSTTFIIFLTAVVMLYFGGTRFSHVLFLFVLIGLLFIFGVQFGLIHSYQIGRIKNYVSNEIPWQLEKAYEAIGNGGIIGSGPALGKYYIHVPQAESDFILATIGESFGYLGIIIVILSYLAIVTSLIKISDEIDNEFIRYFIWGFSTLMMLHVVVNTGVVSGLFPITGIPLPFVSYGGSSILSFSIGFGIIFSEIFIERGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHR9</accession>
    <name>B7IHR9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580063.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1183"/>
    <dbReference type="EnsemblBacteria" id="ACJ75633">
        <property type="protein sequence ID" value="ACJ75633"/>
        <property type="gene ID" value="THA_1183"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1183"/>
    <dbReference type="HOGENOM" id="CLU_663803_0_0_0"/>
    <dbReference type="OMA" id="ERNPSWN"/>
    <dbReference type="OrthoDB" id="2164309at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="414" mass="48711" checksum="C0F91CD303B85482" modified="2009-02-10" version="1">MLIDTLTVFLVSFSFWKINILFVILFTLLFLSFYRFKWLYFLVIILSIIWFKNPFPAFLIYLYEKVKAKEYFLIAFLSVAAFFYSIKVALISSLIVFLYIFRKRSYILLFLIPLIFIPSIFSPIKSVGTVEYEVTQNLQVDMIQSSKVQENSTEPVKKEEVKKQVITDVTVSEDSNLEKSKVSGLFFVSLAGLIILYIFLRLKKDFSFKILAIGFLTFLYAFLIYFVLVPYIAKANNSNLLSFGTSNSVVEKLRPGEYEKNMQATKTEITIEEKPLIKPIYILRFINIISVIVFAISLYILFMFLTAPKKQVEVIKEKENIWKSEDTFEDLIDIEDGRDFIRRAYVYLRAKIFKEFFYLTPYELMNIINDKNLEYFTEIFVKSQYKLEDVTFNLEVAKQKLKELIEKYTAPSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHS5</accession>
    <name>B7IHS5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580069.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1189"/>
    <dbReference type="EnsemblBacteria" id="ACJ75639">
        <property type="protein sequence ID" value="ACJ75639"/>
        <property type="gene ID" value="THA_1189"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1189"/>
    <dbReference type="eggNOG" id="ENOG50328DD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_590428_0_0_0"/>
    <dbReference type="OMA" id="NYYLDIM"/>
    <dbReference type="OrthoDB" id="842633at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="465" mass="54021" checksum="66B879FEE35F1E8A" modified="2009-02-10" version="1">MRKIGVFLLIYLSIFSFSNILNFVPYDFDIFIHFNNEIGSIEKIKNVPFLNFIVSDNGLAFENYLLSYLENVDYESGLKKEIFLDSLSSNILFLSKGGEIRIDENVSFDLNYYIDIIRNLSKDSALIFETENATDLAYYLGALLDEKITLNGNIFNIGDSLFVSKFKNYLIVTGSKSVLDYLVSTYMTDELKFTKKYPEILEKVPSDYWFLGYSKSNALKITFPVETIKTDIKTEYLLFYGRIDESVLKITVNQKSNKFFEKSYVVDDLMENIPVLGNYFGGISVDDSKEAIDFIKPWIFTFESTSVTKIYEITNTILNNSTSTFYVVGDVDDATNVSLAFLFKLSDGKTSIKKMLEKYNAKYDKIKDEWLMNIADDVDIYFFEYGGFFVISNLDKSIYERKARVKRLMDIPAYNFLDRKKNYDVKIFLDVGDLIYKFLGMDIDSKVIFWQEKDGFFVNYYLDIM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHU1</accession>
    <name>RS12_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">30S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpsL</name>
        <name type="ordered locus">THA_1210</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">With S4 and S5 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101428.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHU1"/>
    <dbReference type="SMR" id="B7IHU1"/>
    <dbReference type="STRING" id="484019.THA_1210"/>
    <dbReference type="EnsemblBacteria" id="ACJ75655">
        <property type="protein sequence ID" value="ACJ75655"/>
        <property type="gene ID" value="THA_1210"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1210"/>
    <dbReference type="eggNOG" id="COG0048">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104295_1_2_0"/>
    <dbReference type="OMA" id="SPALEKC"/>
    <dbReference type="OrthoDB" id="1707228at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03368">
        <property type="entry name" value="Ribosomal_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_B">
        <property type="entry name" value="Ribosomal_S12_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_S12/S23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_S12_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="PTHR11652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00981">
        <property type="entry name" value="rpsL_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_1000123529" description="30S ribosomal protein S12">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="modified residue" description="3-methylthioaspartic acid" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <sequence length="124" mass="13733" checksum="7B5F9C234BBE668C" modified="2009-02-10" version="1">MPTINQLVRHGRKVIKEKSKSPALQGHPQKRGVCVRVSTMTPKKPNSALRKIARVRLSNGIEVTAYIPGIGHNLQEHSVVLVRGGRVKDLPGIRYKIIRGALDAAGVENRKQSRSKYGAKRPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHZ5</accession>
    <name>B7IHZ5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HNH endonuclease domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580102.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1264"/>
    <dbReference type="EnsemblBacteria" id="ACJ75709">
        <property type="protein sequence ID" value="ACJ75709"/>
        <property type="gene ID" value="THA_1264"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1264"/>
    <dbReference type="eggNOG" id="COG1403">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036716_1_0_0"/>
    <dbReference type="OMA" id="RGQQIKD"/>
    <dbReference type="OrthoDB" id="1194697at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002711">
        <property type="entry name" value="HNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025938">
        <property type="entry name" value="RRXRR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01844">
        <property type="entry name" value="HNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14239">
        <property type="entry name" value="RRXRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNHc" evidence="1">
        <location>
            <begin position="177"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="423" mass="48921" checksum="80D2E5E02C6C4E48" modified="2009-02-10" version="1">MVYVISKHGKTLMPTKRHGKVRRLLKQGLAKVVRRGPFTIQLLYDTTTYTQPLTVGIDIGSKTVGISAITEKQELLSMEVELRQDIKRLLLERREYRRNRRYRKRRYRKPRFLNRRRYEGWLAPSVQWKVDAHIRLVNLIAKILPVTKIVIEIAPFDTHKIVNPDVEGIQYQEGPQKSFSDVREYCLWRAGYKSELSGKKGILEVHHIIPRSKGGTDNPSNLIVLTAEEHKALHEGKIKIPRSKLEKVRILKDASHVSTIGWHIVNRLKQQYDVGITFGSITKSKRIEMGLEKTHRNDAFVIADGNKNINRTTKWYFGKFFRRQNRSIHKANPIKGGIRPSNTVKEVRGFRRFDKVRYKNQIGIIHGLRSSGYFDIRTLSGEKIHSSAKWSNLKLLEKAKTLMLESRNQLIPLHLNGVSCAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICK7</accession>
    <name>B7ICK7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Lysine 2,3-aminomutase YodO family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1289</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580122.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1289"/>
    <dbReference type="EnsemblBacteria" id="ACJ75734">
        <property type="protein sequence ID" value="ACJ75734"/>
        <property type="gene ID" value="THA_1289"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1289"/>
    <dbReference type="eggNOG" id="COG1509">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032161_3_1_0"/>
    <dbReference type="OMA" id="VTNQCAM"/>
    <dbReference type="OrthoDB" id="557227at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003739">
        <property type="entry name" value="Lys_aminomutase/Glu_NH3_mut"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30538">
        <property type="entry name" value="PTHR30538"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004911">
        <property type="entry name" value="DUF160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01070">
        <property type="entry name" value="PLP-dependent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00238">
        <property type="entry name" value="TIGR00238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="84"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004911-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="370" mass="42952" checksum="FB965FA57E869D8F" modified="2009-02-10" version="1">MQVNYITKIEKVPQLSEEEKNKLKKVTEKYKFRANDYYLKLINWDDPNDPIRNLIIPQIGELEEWGRLDASNEKSYTISKGLQHKYRDTALLLVNDVCGGFCRFCFRKRLFINVGEEVVRDVSEDLEYIKKHKEITNVLLTGGDPLLLATKKLEKIISQIREIDHVQIIRIGSKMVAFNPYRITEDPELIELIKKYSTDEKKIYIMTQFNHPREITKEAIKAVNMLQKAGAILANQTPLIKGVNADWKTLMELFKKLSFIGVPPYYVFQGRPVAGNKPFAVPVEEAYQIFLKAIMNVSGLAKRARFAMSHETGKIEVSALTKEHVIFRYQRAHDPKNAGKIMVYKRNPNAYWFDDYTELVEEYVVENPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICL3</accession>
    <name>B7ICL3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Endo-beta-N-acetylglucosaminidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1295</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580126.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1295"/>
    <dbReference type="CAZy" id="CBM32">
        <property type="family name" value="Carbohydrate-Binding Module Family 32"/>
    </dbReference>
    <dbReference type="CAZy" id="GH85">
        <property type="family name" value="Glycoside Hydrolase Family 85"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75740">
        <property type="protein sequence ID" value="ACJ75740"/>
        <property type="gene ID" value="THA_1295"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1295"/>
    <dbReference type="eggNOG" id="COG3291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4724">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008357_0_0_0"/>
    <dbReference type="OMA" id="NNSKWCA"/>
    <dbReference type="OrthoDB" id="300976at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033925">
        <property type="term" value="F:mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032979">
        <property type="entry name" value="ENGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005201">
        <property type="entry name" value="Glyco_hydro_85"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246">
        <property type="entry name" value="PTHR13246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03644">
        <property type="entry name" value="Glyco_hydro_85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="SSF49299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F5/8 type C" evidence="5">
        <location>
            <begin position="735"/>
            <end position="880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="883" mass="101216" checksum="8F88B60B437F5F0E" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICP3</accession>
    <name>B7ICP3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Diaminopimelate decarboxylase</fullName>
            <shortName evidence="2">DAP decarboxylase</shortName>
            <shortName evidence="2">DAPDC</shortName>
            <ecNumber evidence="2 3">4.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 6" type="primary">lysA</name>
        <name evidence="6" type="ordered locus">THA_1325</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H(+) + meso-2,6-diaminoheptanedioate = CO2 + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:15101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="4.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; L-lysine from DL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.20" evidence="2 3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101660.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1325"/>
    <dbReference type="EnsemblBacteria" id="ACJ75770">
        <property type="protein sequence ID" value="ACJ75770"/>
        <property type="gene ID" value="THA_1325"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1325"/>
    <dbReference type="eggNOG" id="COG0019">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026444_0_1_0"/>
    <dbReference type="OMA" id="YCRSMAS"/>
    <dbReference type="OrthoDB" id="861683at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008836">
        <property type="term" value="F:diaminopimelate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06828">
        <property type="entry name" value="PLPDE_III_DapDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02120">
        <property type="entry name" value="LysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002986">
        <property type="entry name" value="DAP_deCOOHase_LysA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01181">
        <property type="entry name" value="DAPDCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01048">
        <property type="entry name" value="lysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="5">
        <location>
            <begin position="24"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="4">
        <location>
            <begin position="252"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="244"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="384" mass="43906" checksum="713F08FE81780056" modified="2009-02-10" version="1">MKEIFQKVADEIGTPVYIYFEKILEERINKVKNVFEEINFFPTFAAKANNNPAILKILKNHGFGVDILGEGELLACKIASIEGDKIVWNGNGKTKEQKDLMKEYKVKYVNVDSKEEFENLWKNEDDFELFLRVNPDIDAKTHPYISTGLKKHKFGVDFKTAEEILKSQKISGVHIHIGSQITDVKPFKEAIEKTLKLCQKYNIEKINIGGGWGIKYKDENELNTQEYKEEIIPLLKNFKLVINELGRFIIAPAGFLLTRVTLIKKTEKKFFVVTESGMNHLLRPALYNAYHEIEVLNNSKDEILADVVGPLCESGDFLAKERKIKKPEIGELLVIKNAGAYGFSMANNYNGTVRPAEVLITKDGKIKLIRKRETFDELFKNVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICP7</accession>
    <name>B7ICP7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GTP-binding protein YchF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1329</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101667.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1329"/>
    <dbReference type="EnsemblBacteria" id="ACJ75774">
        <property type="protein sequence ID" value="ACJ75774"/>
        <property type="gene ID" value="THA_1329"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1329"/>
    <dbReference type="eggNOG" id="COG0012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018395_0_1_0"/>
    <dbReference type="OMA" id="DFHDLCE"/>
    <dbReference type="OrthoDB" id="603226at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGS" evidence="2">
        <location>
            <begin position="270"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="355" mass="40661" checksum="88193C842A8361BC" modified="2009-02-10" version="1">MKIGIIGLPQVGKTTIFSLLTGIEVDPYSPIHQKGVAKVYDKRVDILSKMYNPKKTTYATLEFYDTPSLDPSDKKSRIQVFNMVQNADALLLVLRAFKNDSVPYPENGETAFKQLKLALDEFIFRDLEIITNRIERLENAKRKLDNREENELKLLKELAKVLENEEFLSRYELTDDQRKLISSYSLVTLRPIIIALNLDEEQFSSNSYETKEEVLELVKEYNFAYIELCGLMEKEIQELDEEERAEFLKDLGIEQTGIERLSKVVYDQLGLISFFTVGSDEVRAWTLKKGSTAVEAAGAIHSDLARGFIKAEVIKYKDLIELGSEKEVKEKGLMKLVGKEHIIEDGDIITIRFNV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICR3</accession>
    <name>RPOB_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit beta</fullName>
            <shortName evidence="1">RNAP subunit beta</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoB</name>
        <name type="ordered locus">THA_1345</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580163.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ICR3"/>
    <dbReference type="SMR" id="B7ICR3"/>
    <dbReference type="STRING" id="484019.THA_1345"/>
    <dbReference type="EnsemblBacteria" id="ACJ75790">
        <property type="protein sequence ID" value="ACJ75790"/>
        <property type="gene ID" value="THA_1345"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1345"/>
    <dbReference type="eggNOG" id="COG0085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_4_3_0"/>
    <dbReference type="OMA" id="FMTWEGY"/>
    <dbReference type="OrthoDB" id="9601at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01321">
        <property type="entry name" value="RNApol_bact_RpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042107">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_ext_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019462">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_external_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010243">
        <property type="entry name" value="RNA_pol_bsu_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="PTHR20856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10385">
        <property type="entry name" value="RNA_pol_Rpb2_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02013">
        <property type="entry name" value="rpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000141745" description="DNA-directed RNA polymerase subunit beta">
        <location>
            <begin position="1"/>
            <end position="1172"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01321"/>
        </source>
    </evidence>
    <sequence length="1172" mass="132196" checksum="3FF4E81F5FB0D67E" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICR9</accession>
    <name>B7ICR9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase, group 2 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1351</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580168.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1351"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75796">
        <property type="protein sequence ID" value="ACJ75796"/>
        <property type="gene ID" value="THA_1351"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1351"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_328661_0_0_0"/>
    <dbReference type="OMA" id="HYGYIWT"/>
    <dbReference type="OrthoDB" id="1379602at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="7"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="498"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="863" mass="102103" checksum="76417468FDB3B714" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICS5</accession>
    <name>B7ICS5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sucrose transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1357</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580174.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1357"/>
    <dbReference type="EnsemblBacteria" id="ACJ75802">
        <property type="protein sequence ID" value="ACJ75802"/>
        <property type="gene ID" value="THA_1357"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1357"/>
    <dbReference type="eggNOG" id="COG2211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038661_0_0_0"/>
    <dbReference type="OMA" id="PMVVDMT"/>
    <dbReference type="OrthoDB" id="1345431at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="6"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="416" mass="46873" checksum="96676EE44AFC1F14" modified="2009-02-10" version="1">MKNYKKLFLLGFGFFGISIVWPLYNAYVPIFLKDFSLSSTSIGFVMTIDNIFAIFMLPLIGVLSDQTRTRFGRRMPYILIGAPLGALFFSLIPFAREMHLLWFFMLNIIFMNFFMALFRSPVIALMPDITPPKYRSQANGVINFMGGLGALLAYFAGKPLYDKNYALPFWLGALIMLIALMLVVVFIKEDEKYKIKEGKKKKIDNVFSKSFSELKVNLKEVFLSKEKSLLMILLSILFWFIGYNALETFFTSYAKFRIGISESTGAFILGFFSLTFMLFSIPAGFIGAKIGRKKTMTIGLAIVILISILSIASVNMINNTSLVTRLLFIYFALGGIGWGMVNVNSLPTVVDMTTEEKLGGYTGLYYFFSMSANIIAPPLAGFFIDILGYDSLLYFSIASFFVATITLQFVRRGDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICS9</accession>
    <name>B7ICS9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1361</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580177.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1361"/>
    <dbReference type="EnsemblBacteria" id="ACJ75806">
        <property type="protein sequence ID" value="ACJ75806"/>
        <property type="gene ID" value="THA_1361"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1361"/>
    <dbReference type="HOGENOM" id="CLU_2902812_0_0_0"/>
    <dbReference type="OrthoDB" id="1170010at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="62" mass="7202" checksum="BED753704F6F5752" modified="2009-02-10" version="1">MESIRKKILKNSIVKLSDEKEIHINQPFVESGLLVFSNDSEILRFAKDILDGYENALGRRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICW4</accession>
    <name>B7ICW4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Extracellular solute-binding protein, family 5, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101784.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1397"/>
    <dbReference type="EnsemblBacteria" id="ACJ75841">
        <property type="protein sequence ID" value="ACJ75841"/>
        <property type="gene ID" value="THA_1397"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1397"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_8_3_0"/>
    <dbReference type="OMA" id="ITTTNIW"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBP_bac_5" evidence="1">
        <location>
            <begin position="86"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="607" mass="68845" checksum="95C0ADF670C13783" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICX9</accession>
    <name>B7ICX9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PhoH protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1412</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101818.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1412"/>
    <dbReference type="EnsemblBacteria" id="ACJ75856">
        <property type="protein sequence ID" value="ACJ75856"/>
        <property type="gene ID" value="THA_1412"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1412"/>
    <dbReference type="eggNOG" id="COG1702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051654_0_0_0"/>
    <dbReference type="OMA" id="PAQMKMV"/>
    <dbReference type="OrthoDB" id="445854at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003714">
        <property type="entry name" value="PhoH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02562">
        <property type="entry name" value="PhoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoH" evidence="3">
        <location>
            <begin position="106"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="315" mass="35761" checksum="3C842853613E3E6F" modified="2009-02-10" version="1">MLKKISIPKDVFVVEVFGQYDNRLRYLKKKFGLEISLRGNDIFIRGDDEKKIEIVESIIKEMINITRDGHLLDKHEFEYLVSEKEEETQEEALSNVVTKTIARGRVKPKTKGQKAYIDAVEKNDIVFVIGPAGTGKTYLASALAVDYLRSGKVSRIILTRPAVEAGEKLGFLPGDLSEKVDPYLRPLFDSLMDMLPLEKFISYREKNIIEIAPLAYMRGRTLNNAFIILDEAQNTTYEQMKMFLTRIGFNSKAIITGDITQIDIKENSGLVMAEKILKGIEGIGFVYLSEADVVRHPLVKKIIKAYEVYEKGVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID27</accession>
    <name>B7ID27_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA 2',3'-cyclic phosphodiesterase</fullName>
            <shortName evidence="1">RNA 2',3'-CPDase</shortName>
            <ecNumber evidence="1">3.1.4.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-end 2'-phospho-ribonucleotide-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11828"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17353"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173113"/>
            <dbReference type="EC" id="3.1.4.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2H phosphoesterase superfamily. ThpR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.58" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101929.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1461"/>
    <dbReference type="EnsemblBacteria" id="ACJ75904">
        <property type="protein sequence ID" value="ACJ75904"/>
        <property type="gene ID" value="THA_1461"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1461"/>
    <dbReference type="eggNOG" id="COG1514">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081251_3_4_0"/>
    <dbReference type="OMA" id="HITLRFL"/>
    <dbReference type="OrthoDB" id="1696971at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008664">
        <property type="term" value="F:2'-5'-RNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01940">
        <property type="entry name" value="RNA_CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004175">
        <property type="entry name" value="RNA_CPDase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561">
        <property type="entry name" value="PTHR35561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="SSF55144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02258">
        <property type="entry name" value="2_5_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="HXTX 1" evidence="1">
        <location>
            <begin position="40"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 2" evidence="1">
        <location>
            <begin position="127"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="190" mass="22115" checksum="9A92BFF8FCA7105E" modified="2009-02-10" version="1">MRTFIAIDINSEVRSIAEEVIEKLKKMGFKASWTSPENLHLTLFFMGDLSEEKVDLLAQRLHKRLAGFPSFSYEVSDFGFFRFKHLPRVFFLKVTQDKILQTLYLEMRSELKKLRLSFDDKGNFVPHITLGRLKFSPENWEELIKGITVPKTIVSVDKVTIYSSTLTPAGPIYKWLYKVKFEGGLEKNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID57</accession>
    <name>B7ID57_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycerophosphodiester phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580257.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1493"/>
    <dbReference type="EnsemblBacteria" id="ACJ75934">
        <property type="protein sequence ID" value="ACJ75934"/>
        <property type="gene ID" value="THA_1493"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1493"/>
    <dbReference type="eggNOG" id="COG0584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030006_3_3_0"/>
    <dbReference type="OMA" id="NVPMEAI"/>
    <dbReference type="OrthoDB" id="1542724at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="SSF51695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="230" mass="27151" checksum="5EBCA32B56683AAD" modified="2009-02-10" version="1">MKILGHRGYPEKYVENTIDSFKAAIDYGADGVELDVYMTLDEKIVVTHDSNLRRVFNVDLEVTKSFLKEIKEVAPVPELIEVFNVLPKEKLINVEIKDKEKGEKIVEYVIENKKHDIIFSSFEHEIILKCVEKYKDEKFGLLFDERHQNLSFEDIRELFENHNIYSAHLPIDLLYIDKEKFFSLVKYLKKIEKKIVFWTVNKIEEVEVIKDFADIIITNSVEKMVGYIKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID76</accession>
    <name>COAX_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type III pantothenate kinase</fullName>
            <ecNumber evidence="1">2.7.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">PanK-III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pantothenic acid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coaX</name>
        <name type="ordered locus">THA_1512</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NH4(+)</name>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
        </cofactor>
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">A monovalent cation. Ammonium or potassium.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type III pantothenate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102036.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ID76"/>
    <dbReference type="SMR" id="B7ID76"/>
    <dbReference type="STRING" id="484019.THA_1512"/>
    <dbReference type="EnsemblBacteria" id="ACJ75953">
        <property type="protein sequence ID" value="ACJ75953"/>
        <property type="gene ID" value="THA_1512"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1512"/>
    <dbReference type="eggNOG" id="COG1521">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066627_1_1_0"/>
    <dbReference type="OMA" id="HEPWLTL"/>
    <dbReference type="OrthoDB" id="2039419at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00352"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01274">
        <property type="entry name" value="Pantothen_kinase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004619">
        <property type="entry name" value="Type_III_PanK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34265">
        <property type="entry name" value="PTHR34265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03309">
        <property type="entry name" value="Pan_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00671">
        <property type="entry name" value="baf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000140260" description="Type III pantothenate kinase">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="6"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="101"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01274"/>
        </source>
    </evidence>
    <sequence length="249" mass="28213" checksum="FB2DD1BA141BCCD6" modified="2009-02-10" version="1">MLLLFDVGNTHTTIALTKDGKSFDIKRVSTHSIQTEDELYVFLKMFYRNDFKDIVVSSVVPNINYIFEFFAEKYLNKKAIFVSAKEYNEIIWNVNTPEEIGADRVVDVIAASRDYGKDAIVVDFGTAITIEVLKENRYEGGVIIPGFNMMVNALFKGTAKLPKVELKPSDTFVGKDTGSNIRIGVINTILGGIDYTIERIKSDYDLKSAPIIYTGGQSKLIREYLKKDIIYDLELGLRGIYYFYENIAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID81</accession>
    <name>B7ID81_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamate racemase</fullName>
            <ecNumber evidence="2">5.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">murI</name>
        <name evidence="7" type="ordered locus">THA_1517</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamate = D-glutamate</text>
            <dbReference type="Rhea" id="RHEA:12813"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29986"/>
            <dbReference type="EC" id="5.1.1.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.1.1.3" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102046.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1517"/>
    <dbReference type="EnsemblBacteria" id="ACJ75958">
        <property type="protein sequence ID" value="ACJ75958"/>
        <property type="gene ID" value="THA_1517"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1517"/>
    <dbReference type="eggNOG" id="COG0796">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052344_0_2_0"/>
    <dbReference type="OMA" id="MPWGPRT"/>
    <dbReference type="OrthoDB" id="1718671at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008881">
        <property type="term" value="F:glutamate racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033134">
        <property type="entry name" value="Asp/Glu_racemase_AS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004391">
        <property type="entry name" value="Glu_race"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198:SF2">
        <property type="entry name" value="PTHR21198:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53681">
        <property type="entry name" value="SSF53681"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00067">
        <property type="entry name" value="glut_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00924">
        <property type="entry name" value="ASP_GLU_RACEMASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="263" mass="30012" checksum="EAE32A98C1FB1A1B" modified="2009-02-10" version="1">MKIGLFDSGIGGISVLKKLTYINGAHYIYIADTERAPYGIKTPEVLESFIFEFAEFFHKKGVNEIFAACNTTDSIILEKNLDLGIKYNSIIKAAIQAPKSEKVGVIGTNITVSNGIYKKYLEMENKEVYQKSTQLFVSLVEEGIFYGRMVEAIANYYLLPLKKKNIEELILGCTHFPFLKGVISRVLPKVRIIDPAEELSKKLAKNNSKKAFVEFYVTGDPKVFEKKLKRINFRVPYTINKFKTKKVIQIEKPNYSDRVVGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDB9</accession>
    <name>B7IDB9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">NADP-reducing hydrogenase, subunit b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102237.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1558"/>
    <dbReference type="EnsemblBacteria" id="ACJ75996">
        <property type="protein sequence ID" value="ACJ75996"/>
        <property type="gene ID" value="THA_1558"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1558"/>
    <dbReference type="eggNOG" id="COG3411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126515_4_0_0"/>
    <dbReference type="OMA" id="TQTGCMG"/>
    <dbReference type="OrthoDB" id="1412344at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="124" mass="13456" checksum="5DE0196A5D20C6BF" modified="2009-02-10" version="1">MGKIKSLEELMKIKENTLKNVKMREEGKRGRIIVAMGTCGIAAGAKDTLKAIVETLSELNIDDISVVQSGCMGLCEVEPTIKVELDGEIPVIYGHVTPENAKRIIKSHIVAGEQVGELIVKRGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDD1</accession>
    <name>LEXA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LexA repressor</fullName>
            <ecNumber evidence="1">3.4.21.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lexA</name>
        <name type="ordered locus">THA_1570</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of Ala-|-Gly bond in repressor LexA.</text>
            <dbReference type="EC" id="3.4.21.88"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S24 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.88" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102298.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDD1"/>
    <dbReference type="SMR" id="B7IDD1"/>
    <dbReference type="STRING" id="484019.THA_1570"/>
    <dbReference type="MEROPS" id="S24.001"/>
    <dbReference type="EnsemblBacteria" id="ACJ76008">
        <property type="protein sequence ID" value="ACJ76008"/>
        <property type="gene ID" value="THA_1570"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1570"/>
    <dbReference type="eggNOG" id="COG1974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_45_1_0"/>
    <dbReference type="OMA" id="HVWLLPH"/>
    <dbReference type="OrthoDB" id="1933795at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06529">
        <property type="entry name" value="S24_LexA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00015">
        <property type="entry name" value="LexA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006200">
        <property type="entry name" value="LexA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039418">
        <property type="entry name" value="LexA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006199">
        <property type="entry name" value="LexA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006197">
        <property type="entry name" value="Peptidase_S24_LexA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01726">
        <property type="entry name" value="LexA_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00726">
        <property type="entry name" value="LEXASERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00498">
        <property type="entry name" value="lexA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_1000192779" description="LexA repressor">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="active site" description="For autocatalytic cleavage activity" evidence="1">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="active site" description="For autocatalytic cleavage activity" evidence="1">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="1">
        <location>
            <begin position="84"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00015"/>
        </source>
    </evidence>
    <sequence length="198" mass="22460" checksum="3D0419FB72C906D3" modified="2009-02-10" version="1">MKELTNRQKMVLDFITSYIQQNGYSPSIRDIAKHFKLTPRGAHIHVLALEKKGYITRNPKNSRSISLVKRPETVSIPVKGKISAGQGIEMFELVDEEIEIPVRMINGYGNYFALRVEGTSMIEAHIIDGDYVILKKQYRIPNGQIAAVVFDNKVTLKRFYHKNDKVELVPENSSMSPIICDAKDVKVIGKLVGVIRIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDF4</accession>
    <name>B7IDF4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1593</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102344.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1593"/>
    <dbReference type="EnsemblBacteria" id="ACJ76031">
        <property type="protein sequence ID" value="ACJ76031"/>
        <property type="gene ID" value="THA_1593"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1593"/>
    <dbReference type="eggNOG" id="ENOG502ZPZ3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_600968_0_0_0"/>
    <dbReference type="OMA" id="QAFGAIH"/>
    <dbReference type="OrthoDB" id="1571850at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="472" mass="53393" checksum="9405FF63B5E1F089" modified="2009-02-10" version="1">MKKILFTILILFALFSSIGFAVTIYPLVNRLNVEANQRTIELTINVANNSPSIADVNVEISDFVIEGSKYLYDIPNYAFSIKNWITIESTHLILQPGQSIDYPIKINIPSGFKGAQAFGAIHFKQKGKQSEVFETIFDYISLIILDFPTVKNIKAQIQDVSIYDLTTTASKTLIDKYGNFGTVIDINVKNNGNAVLALNGEMRLISREINRIITSIPLNSNNFVVFPDRANNFEFFIPYVLPKGDFEIQMDGISQNVRVTAFKKLKIEGKSSDRVAVMIDPEIILINVKRTIENSKITIQNLSPLSTQFSLSTISKAINILPGKIRLSPYSKLNGFVKFDSRLEKLKDGDNVFKINIDSENEISLFKNPKIVLRYGNLEPNIESFITDVATNTFALNVKNTGNTILIFKVIRTEKLQSNDLTNEIILFPGENKKVFLEIPYNEIIKNSTFIMYKIYGENDFKYQKNIGDVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDF5</accession>
    <name>B7IDF5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580283.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1594"/>
    <dbReference type="EnsemblBacteria" id="ACJ76032">
        <property type="protein sequence ID" value="ACJ76032"/>
        <property type="gene ID" value="THA_1594"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1594"/>
    <dbReference type="eggNOG" id="ENOG502ZUIT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144857_0_0_0"/>
    <dbReference type="OMA" id="ISEFDIC"/>
    <dbReference type="OrthoDB" id="2126089at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857348" evidence="1">
        <location>
            <begin position="21"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="153" mass="17085" checksum="D6E28D995F25B42B" modified="2009-02-10" version="1">MRKMLITLLSLFALFIFADATSVHVPGCDWEEYTIDSSITIFVHQWMDLDYEWLIADFDICDYTQTGDVDVLTFTLDSNAPVNVIPYADYNGLDAYIDADVYVTKDGNPFVPTSPIDDGVYLIGFTINSIDPDLPAGDYPVVLSLTFVPTVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDN9</accession>
    <name>B7IDN9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1679</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102504.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76116">
        <property type="protein sequence ID" value="ACJ76116"/>
        <property type="gene ID" value="THA_1679"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1679"/>
    <dbReference type="eggNOG" id="COG2013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040551_4_1_0"/>
    <dbReference type="OMA" id="AMVYMKG"/>
    <dbReference type="OrthoDB" id="1063432at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="SSF51219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00266">
        <property type="entry name" value="TIGR00266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="224" mass="24396" checksum="4B55CC843C1DD0AD" modified="2009-02-10" version="1">MLFKTDFRGSYAILFVELEIGETIVAEPGAMVSMEGDIEVNTSTGGIFKALKRAFLGGEHFFLNKYISHSKSKISFAPLLPGDIEVIDIKNGFFLQSTSYLASQETISIDTKFGGFKTFFSGEGFFLLKLSGYGKAAISAFGGIYHKELEPGEAITVDTGHIVGFDETVKYSVRTFGGIKSTLFGGEGLVVDLVGPGRVYIQTRNSPAFINWIKNLIPRDTASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDP0</accession>
    <name>B7IDP0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bax protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580343.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1680"/>
    <dbReference type="CAZy" id="GH73">
        <property type="family name" value="Glycoside Hydrolase Family 73"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76117">
        <property type="protein sequence ID" value="ACJ76117"/>
        <property type="gene ID" value="THA_1680"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1680"/>
    <dbReference type="eggNOG" id="COG2992">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061344_1_0_0"/>
    <dbReference type="OMA" id="NLFGQWC"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002901">
        <property type="entry name" value="MGlyc_endo_b_GlcNAc-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01832">
        <property type="entry name" value="Glucosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LYZ2" evidence="1">
        <location>
            <begin position="129"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="258" mass="30248" checksum="765B796550897871" modified="2009-02-10" version="1">MRKVTFSVIIIAFLFLNFYTIDSEFEYFQFFESEINVKFDDWKELKKYFEKIGYFSIKNPPNIIVKSLPKNLSEAPVAEKKELFVKIMIPLIRKVNQEILMERKEILKAKENGDLKVLEFYMKKYDAKDYEELLLKVNTIPEDIALAQAAVESAWGTSRFAIQANNIFGEWTFTPGTGIVPNDRPDGEIYEVEYFKSLLDSMRSYALNLNKLPYYEDFRLIRAGIKKGHPADGLINYSQMREKYVEIIKTVIKNLPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDS2</accession>
    <name>B7IDS2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase III subunit gamma/tau</fullName>
            <ecNumber evidence="4">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dnaX</name>
        <name evidence="7" type="ordered locus">THA_1713</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the DnaX/STICHEL family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102571.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1713"/>
    <dbReference type="EnsemblBacteria" id="ACJ76149">
        <property type="protein sequence ID" value="ACJ76149"/>
        <property type="gene ID" value="THA_1713"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1713"/>
    <dbReference type="eggNOG" id="COG2812">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006229_0_8_0"/>
    <dbReference type="OMA" id="AMHYELM"/>
    <dbReference type="OrthoDB" id="556582at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18137">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012763">
        <property type="entry name" value="DNA_pol_III_sug/sutau_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045085">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02397">
        <property type="entry name" value="dnaX_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="35"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="480" mass="54849" checksum="90B65004F288CD1F" modified="2009-02-10" version="1">MQALYRKYRPKLFSEVVGQNHVKTLFLNSLKNGEISHAYIFAGPRGTGKTTVARILAKSLNCENKNGVEPCNKCNTCQSIDNGSFMDVLEIDAASNRGIDEIRKIRETVGYHAAQGNYKVYIIDEVHMLTKEAFNALLKTLEEPPSNVVFILATTNPEKIPQTIISRCQVIDFKNLTIEDIIKRLEFVCKKENINISQEALNEIAKRANGGMRDALTILEQVSKSSSKDITIEDVRETLGLVPIEVINEMIDSIKIGDIKKTTEIIEKVYFSGKDPEVFLMQLLEEMILKIESGDYELIEFLKPLSEILKDIKYSEEKLILIKLGFLSVMKLKPPRENIKKNVEKAEENEPIPESKLNDKDLESTQNVENKEKKIENKENISKIMEYLKLHGDLSLYVGLSFSKIEELPDRVKITFPPEKKIHYELVRSKIHELEKLYTSKFGIFKPFEVIIKENSKEDDVLNKLKSLFGNNFIVEGDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDU0</accession>
    <name>B7IDU0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methenyltetrahydrofolate cyclohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1732</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580375.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1732"/>
    <dbReference type="EnsemblBacteria" id="ACJ76167">
        <property type="protein sequence ID" value="ACJ76167"/>
        <property type="gene ID" value="THA_1732"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1732"/>
    <dbReference type="eggNOG" id="COG3404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088419_1_0_0"/>
    <dbReference type="OMA" id="VAMVARY"/>
    <dbReference type="OrthoDB" id="1965647at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007044">
        <property type="entry name" value="Cyclodeamin/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036178">
        <property type="entry name" value="Formintransfe-cycloase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04961">
        <property type="entry name" value="FTCD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101262">
        <property type="entry name" value="SSF101262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FTCD_C" evidence="2">
        <location>
            <begin position="7"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="168"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="207" mass="23298" checksum="E4AFAA6A93FFF06E" modified="2009-02-10" version="1">MNVERTTLKEFCDMVADKTPTPGGGAVGAIVAALAAALNQMVANLTIGKKKYADFEEVMEEVLENMNYSREKLQQLAYDDIKAFNKVMEAIKIPKDDPTRSEKLQKALKEAADIPFELAREARNVLKFSQVTSKHGNKNAISDAYSSAELAYAAFRIGMYNVLINLSSIKDEEFVRKYKEELEELKNEVEGIYKSIRELVKENGFEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDU7</accession>
    <name>RL9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplI</name>
        <name type="ordered locus">THA_1739</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580380.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDU7"/>
    <dbReference type="SMR" id="B7IDU7"/>
    <dbReference type="STRING" id="484019.THA_1739"/>
    <dbReference type="EnsemblBacteria" id="ACJ76174">
        <property type="protein sequence ID" value="ACJ76174"/>
        <property type="gene ID" value="THA_1739"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1739"/>
    <dbReference type="eggNOG" id="COG0359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078938_3_0_0"/>
    <dbReference type="OMA" id="MKIILTH"/>
    <dbReference type="OrthoDB" id="1959318at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.430.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00503">
        <property type="entry name" value="Ribosomal_L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_L9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_L9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020594">
        <property type="entry name" value="Ribosomal_L9_bac/chp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020069">
        <property type="entry name" value="Ribosomal_L9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036791">
        <property type="entry name" value="Ribosomal_L9_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_L9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_L9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="PTHR21368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03948">
        <property type="entry name" value="Ribosomal_L9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55653">
        <property type="entry name" value="SSF55653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="SSF55658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00158">
        <property type="entry name" value="L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00651">
        <property type="entry name" value="RIBOSOMAL_L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000126984" description="50S ribosomal protein L9">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00503"/>
        </source>
    </evidence>
    <sequence length="151" mass="16687" checksum="33B06A7290A87F77" modified="2009-02-10" version="1">MKVVLLKDVAKIGKKGEVKNVSDGYARNFLIPKGLALEATPAVMKQLKAQKMKEEEEKKKIKQESEELLKLLQKHLYKIPVKTGGSGKLFGALTNADIAKAISEKTGKDIDKKHIVLNKPIKELGLYEITVKLPEGITGKIKVEVVQEGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDY9</accession>
    <name>B7IDY9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sensor histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1785</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580410.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1785"/>
    <dbReference type="EnsemblBacteria" id="ACJ76216">
        <property type="protein sequence ID" value="ACJ76216"/>
        <property type="gene ID" value="THA_1785"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1785"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3887">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_2_0"/>
    <dbReference type="OMA" id="WHKLIRI"/>
    <dbReference type="OrthoDB" id="1827824at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="138"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="247"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="465" mass="53874" checksum="FE8B1E88B4C6A8F2" modified="2009-02-10" version="1">MNLDLLFSSFMHEIGKATSEMEVYKALLNSVKSVLNYEKALVMKEDKIVYYEPEMLDIQKFEDYIAWIKQRLLPAFFSEEDKFIGIIPIVKQNKLLGVILLLTKNEPNNEILNLLQTFAFLTGVTVENLILVDQIKKSEKFMSDVLNSINEGIFVLNENGRIEFLNDFAKEILDSAPHINEFLKSAINDERKILTKEFNNHYYTIVKVKFNFSNETKYVVTFNNVTYEIELEKLKQLDKMKTEFVANISHELRTPLTAIKAYTETLLNMEVDRESQREFLETVYEQSERLESLLNDLLDFTLIESGTMELEYSEFDLCELIKEVVENLKQLAEKYKVKINVSCESISISADKRRIKQAIHNLVDNSIKFSDKSKEERYVNISVEKKEENLSIIIEDNGIGISQEDKEKIFEKFYRGDRSLTYEVPGTGLGLTIVQEIIKLHGGKINVNSTLGEGTTFELVIPYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE12</accession>
    <name>B7IE12_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1808</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580432.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1808"/>
    <dbReference type="EnsemblBacteria" id="ACJ76239">
        <property type="protein sequence ID" value="ACJ76239"/>
        <property type="gene ID" value="THA_1808"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1808"/>
    <dbReference type="eggNOG" id="COG3383">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018240_2_1_0"/>
    <dbReference type="OMA" id="CMRCIQV"/>
    <dbReference type="OrthoDB" id="323168at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009016">
        <property type="entry name" value="Fe_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003149">
        <property type="entry name" value="Fe_hydrogenase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036991">
        <property type="entry name" value="Fe_hydrogenase_ssu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013352">
        <property type="entry name" value="Fe_hydrogenase_subset"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02256">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00902">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53920">
        <property type="entry name" value="SSF53920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02512">
        <property type="entry name" value="FeFe_hydrog_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="8">
        <location>
            <begin position="78"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="136"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="179"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ76239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="583" mass="65131" checksum="1DD5B2872F3EED6C" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE16</accession>
    <name>B7IE16_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD synthase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580436.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1812"/>
    <dbReference type="EnsemblBacteria" id="ACJ76243">
        <property type="protein sequence ID" value="ACJ76243"/>
        <property type="gene ID" value="THA_1812"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1812"/>
    <dbReference type="eggNOG" id="COG0037">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_885475_0_0_0"/>
    <dbReference type="OMA" id="VKIIGPL"/>
    <dbReference type="OrthoDB" id="1276643at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_synthase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="342" mass="38679" checksum="EF1E8FDAB6936AB3" modified="2009-02-10" version="1">MSTEKLVENIKNEIKEVVKEYGNKNVVIAFSGGLDSTVAALLTKEALGPENVELANVVYGPFTYKRSIEIVKKSAEKIGLKITFLESLYQKEIWKNGPSCNMCTKTVKMNTVKKYAKDRLVITGSNQSDSWGKTGLKVFNGLYAPLGDLNKSEIQNILDFYSFKLERIGENSKREGCKLKHLLKIMTNLDYHGKAVDFANEILLENVPKNLELANVKILGPLSKNIAIINVKPMVDNINEIKKKIENIDVIDEVIVAKKPLILHVIANPSIYRVKNSRYWIEVGKLQPEFAVPIKVIWKESKNNKLRTIQVVGVEEWKDYDPRNLKINLGTDLEMKNSCSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE31</accession>
    <name>B7IE31_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-sigma factor antagonist</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1827</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the anti-sigma-factor antagonist family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102775.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1827"/>
    <dbReference type="EnsemblBacteria" id="ACJ76258">
        <property type="protein sequence ID" value="ACJ76258"/>
        <property type="gene ID" value="THA_1827"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1827"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115403_6_4_0"/>
    <dbReference type="OMA" id="MNLAINI"/>
    <dbReference type="OrthoDB" id="1864829at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043856">
        <property type="term" value="F:anti-sigma factor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003658">
        <property type="entry name" value="Anti-sigma_ant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00377">
        <property type="entry name" value="ant_ant_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="3">
        <location>
            <begin position="4"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="110" mass="12447" checksum="6BCCF2113547B3F6" modified="2009-02-10" version="1">MNNTKVEFIEKDQYTIAKVTGDIDAYHSADVKKDIREKMLATEKGKFVIDLSEVNYIDSAGLGSLVAILKDARLNNKELALLSPKQSVMRVFEMTRLDKVFHIISSIEEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE38</accession>
    <name>RL34_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmH</name>
        <name type="ordered locus">THA_1834</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102791.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IE38"/>
    <dbReference type="SMR" id="B7IE38"/>
    <dbReference type="STRING" id="484019.THA_1834"/>
    <dbReference type="EnsemblBacteria" id="ACJ76265">
        <property type="protein sequence ID" value="ACJ76265"/>
        <property type="gene ID" value="THA_1834"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1834"/>
    <dbReference type="eggNOG" id="COG0230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129938_2_0_0"/>
    <dbReference type="OrthoDB" id="2069770at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00391">
        <property type="entry name" value="Ribosomal_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000271">
        <property type="entry name" value="Ribosomal_L34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020939">
        <property type="entry name" value="Ribosomal_L34_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14503">
        <property type="entry name" value="PTHR14503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00468">
        <property type="entry name" value="Ribosomal_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01030">
        <property type="entry name" value="rpmH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00784">
        <property type="entry name" value="RIBOSOMAL_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000196132" description="50S ribosomal protein L34">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="44" mass="5348" checksum="72CDE27AAF60DBBE" modified="2009-02-10" version="1">MKRTYQPSRIKRKRTHGFLARKKTAGGRKVLKNRRRKGRWRLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE48</accession>
    <name>B7IE48_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RecQ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1844</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580457.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1844"/>
    <dbReference type="EnsemblBacteria" id="ACJ76275">
        <property type="protein sequence ID" value="ACJ76275"/>
        <property type="gene ID" value="THA_1844"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1844"/>
    <dbReference type="eggNOG" id="COG0514">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1040">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_280586_0_0_0"/>
    <dbReference type="OMA" id="CYLIFTE"/>
    <dbReference type="OrthoDB" id="54580at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="435"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="648"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="1119" mass="132985" checksum="EB89B9021DC0C057" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE68</accession>
    <name>B7IE68_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ARMT1-like_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102861.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1869"/>
    <dbReference type="EnsemblBacteria" id="ACJ76295">
        <property type="protein sequence ID" value="ACJ76295"/>
        <property type="gene ID" value="THA_1869"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1869"/>
    <dbReference type="eggNOG" id="COG1578">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071520_0_0_0"/>
    <dbReference type="OMA" id="FLLKAKC"/>
    <dbReference type="OrthoDB" id="1714760at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014444">
        <property type="entry name" value="PH1575-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006593">
        <property type="entry name" value="UCP006593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="SSF111321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARMT1-like_dom" evidence="1">
        <location>
            <begin position="4"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="293" mass="33447" checksum="91C6D78D000D3EE2" modified="2009-02-10" version="1">MKAISKCLTCHVNQAQRILDKFVISEHEKWNILKKVLNDLSQLNYNLKPIDIAEVMYEKLENYLNKSDLYSEEKKKSNDAALSLYKDFKSRILDSSDPLYEAAKLSVAGNLIDFGAKKGSTEELFKQVIDIWNQPFSIDDFEEFKKSLFSSKNLLFIADNAGELVFDMLFIEIIKETLHDIEIFVALKGKPIINDATVDDGKYIGIEKFATIIDTKLKTAGASLPKSSEEFRRLFYDSDIIIAKGQGNFEGLSEESRNNLFFALVSKCEVISDFIGVEKNSKLFMNSKRIRQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEB1</accession>
    <name>B7IEB1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1914</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102930.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1914"/>
    <dbReference type="EnsemblBacteria" id="ACJ76338">
        <property type="protein sequence ID" value="ACJ76338"/>
        <property type="gene ID" value="THA_1914"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1914"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_0"/>
    <dbReference type="OMA" id="WEVANVI"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="240" mass="26386" checksum="A5E0BCAAA7A9D567" modified="2009-02-10" version="1">MENKVALITGGARNIGKSLAIGFLNNGYKVAVIDHNKELLDKLPENGNLLKFFGDVSNEKDVIKFIEYIEKNFKRLDVIINNAAISNFKNFLEITLEEWEKVIKVNLTGYFLITKYGVPLILKTSGKGSIINIASTRAIMSEKGNEAYSASKAGIIGLTHALANSLGPNIRVNAISPGWILHEGEQISQEEHMQHLVGRAGTPNDILELALFLANEKKSGFITGQNIVIDGGMTKKMIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEE3</accession>
    <name>B7IEE3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tryptophan synthase</fullName>
            <ecNumber evidence="7">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">THA_1948</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580496.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1948"/>
    <dbReference type="EnsemblBacteria" id="ACJ76370">
        <property type="protein sequence ID" value="ACJ76370"/>
        <property type="gene ID" value="THA_1948"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1948"/>
    <dbReference type="eggNOG" id="COG1350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042858_1_0_0"/>
    <dbReference type="OrthoDB" id="912282at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006316">
        <property type="entry name" value="Trp_synth_b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF6">
        <property type="entry name" value="PTHR48077:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500824">
        <property type="entry name" value="TrpB_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01415">
        <property type="entry name" value="trpB_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="PALP" evidence="13">
        <location>
            <begin position="76"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ76370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="420" mass="46678" checksum="904E3C65C35D0AF1" modified="2009-02-10" version="1">MREYVHLNYDEMPKSWYNVLPDLPFKLDPPLDPETQKPMTPEKLLKIFPGPLLEQEANDVDRFIDMPEEILKEYAVYRPTPLIRAKFLEEFLETPVKIYYKYEGVSPTGSHKTNTALAQAYFNKISGTEILYTETGAGQWGSALSYAGVKYGLRVNVYMVRVSYLQKPARKNLINLFEGNVTPSPSRNTKVGRKYPEDHPGSLGIAISEAMEEVFDKDNAKYALGSVLNHVLLHQTVIGLEIKKQLEKLELKPDIIIGCHGGGSNFGGTILPFIPEKLSGENLRFIACEPESCPTLTKGEYRYDFGDTAGFTPMLKMYTLGKDFIPPSIHAGGLRYHGAAPIISALLKNNLIEAQTFSQDETFKAAKLFSKIEGIIPAPESAHAIAGAIREALNAKKEGKGKVIVFTLSGHGLFDLNAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEH3</accession>
    <name>B7IEH3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1979</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580524.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1979"/>
    <dbReference type="EnsemblBacteria" id="ACJ76400">
        <property type="protein sequence ID" value="ACJ76400"/>
        <property type="gene ID" value="THA_1979"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1979"/>
    <dbReference type="HOGENOM" id="CLU_2221969_0_0_0"/>
    <dbReference type="OrthoDB" id="2008048at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="106" mass="12389" checksum="0C585AB7B53708CE" modified="2009-02-10" version="1">MIGFLGWLNVSLLVFNFISFPLRLLNQKLKNVKLAVLIRFLKKYHKLTGLFLIISGFVHGFLALGYSFYFHTGSLLWWTIILMFLFYILHPGQCKNYFSINLIILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEH8</accession>
    <name>B7IEH8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1984</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580528.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1984"/>
    <dbReference type="EnsemblBacteria" id="ACJ76405">
        <property type="protein sequence ID" value="ACJ76405"/>
        <property type="gene ID" value="THA_1984"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1984"/>
    <dbReference type="eggNOG" id="COG5255">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101347_0_0_0"/>
    <dbReference type="OMA" id="IENCIMN"/>
    <dbReference type="OrthoDB" id="1839900at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="SSF55144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="229" mass="27251" checksum="2063162A18077DC1" modified="2009-02-10" version="1">MKLNFNKYIEKLKELKENSLVNLLKEWNPKNELFLKVDEKGNYKDFKGDTIIFFLEEEEDKKKVLNIQKKLYSKLGNYLAPPLSEKYFHMTLHDLCNYNITKEIENCIMNNKEKARKILEEIKSSKKIKMKSLGLYNGGSAIGIMFSPTNEEELNLLLNTRKKFDTLIKQNDFYIPHVTLGYYLPIDYNDKIRRKIYNTLVQINADLEIAINFKNLEYVIFENMDMYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEI8</accession>
    <name>B7IEI8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1994</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TspO/BZRP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103070.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1994"/>
    <dbReference type="EnsemblBacteria" id="ACJ76415">
        <property type="protein sequence ID" value="ACJ76415"/>
        <property type="gene ID" value="THA_1994"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1994"/>
    <dbReference type="eggNOG" id="COG3476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091805_2_0_0"/>
    <dbReference type="OMA" id="GLNLIWM"/>
    <dbReference type="OrthoDB" id="1679387at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15904">
        <property type="entry name" value="TSPO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038330">
        <property type="entry name" value="TspO/MBR-related_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004307">
        <property type="entry name" value="TspO_MBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057">
        <property type="entry name" value="PTHR10057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03073">
        <property type="entry name" value="TspO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005859">
        <property type="entry name" value="PBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="160" mass="18536" checksum="F6DB1756247AC682" modified="2009-02-10" version="1">MEKQNKKKIFKLILSIVLTLLIGFIGSLITNKSLDTWYNTIKKPSFNPPNWLFAPVWTILFIMIGLSFYFVWERGFGNKVKTLLFVFLLQLVLNLLWSYSFFGLTNPLLAFIVIIILWIAILINIIIFYKVSKIAGFLLIPYILWVSFASILNLAIVILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEK7</accession>
    <name>B7IEK7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_13</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM86 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_13"/>
    <dbReference type="EnsemblBacteria" id="ACJ74521">
        <property type="protein sequence ID" value="ACJ74521"/>
        <property type="gene ID" value="THA_13"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_13"/>
    <dbReference type="eggNOG" id="COG3714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1137584_0_0_0"/>
    <dbReference type="OMA" id="MLVQLAW"/>
    <dbReference type="OrthoDB" id="1768508at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012506">
        <property type="entry name" value="YhhN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31885">
        <property type="entry name" value="PTHR31885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07947">
        <property type="entry name" value="YhhN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="244" mass="28102" checksum="73AAE17A58436DA1" modified="2009-02-10" version="1">MFLNKPLPNTNYITYFSTNLVGTRSDVEQFFSWHKNKKLITTFSFSLIVVIFAILTIFIKKMPKSKVIFKVLTTFTIFLIALLNSSGSEFLIVTGLLFGMIGDFLLEFEDKFLHGMFSFLIGHIFYSLSFLIKFGIPNILVFFIILPILLILYFVILFKKAENFKLPILVYTVAIGIMFSFTFAPAFKDIYYLRCMLPLAGGLFVFSDFLIAIEKFVRKIKYSEIIILGTYFLAQLIIALSTIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEP9</accession>
    <name>B7IEP9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="3 7">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">lpdA</name>
        <name evidence="10" type="ordered locus">THA_55</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + NAD(+) = (R)-N(6)-lipoyl-L-lysyl-[protein] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="5 7">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="3 7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579319.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_55"/>
    <dbReference type="EnsemblBacteria" id="ACJ74563">
        <property type="protein sequence ID" value="ACJ74563"/>
        <property type="gene ID" value="THA_55"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_55"/>
    <dbReference type="eggNOG" id="COG1249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_3_0"/>
    <dbReference type="OMA" id="DAKYGEW"/>
    <dbReference type="OrthoDB" id="267896at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="9">
        <location>
            <begin position="4"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="8">
        <location>
            <begin position="332"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="174"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="304"/>
            <end position="307"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="6">
        <location>
            <begin position="39"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="447" mass="49239" checksum="950EB3B062770C75" modified="2009-02-10" version="1">MYEAIVIGGGPGGYVAAIRLSQLGKKVAIVEKEEFGGTCTNKGCIPTKAMLTASHLFTEINEKAKKFGILVDNVSYDLSLIMKHMQKSVTMSRKGIEFLMKKNKIDVFKDKAVLKDKNTVLLENSGQEIQGENIILAHGSVPVIFSPFDQIEGIWTSDDVFKMEKMPESILIIGGGVIGVEFATFFSSFGVDVTVVELAEHILPYEDSDVAEEIKKALKKNKVKIIEGEKVEGVEKKGEKYIAKVAGKEIEVEKVLLAVGRRPNISEDIKALGIEIERGVKTDKHMRTNIDNIYAIGDIRGQIMLAHVAMYEGIVAAHNIAGEEIEMDYRAVPSIIFSNPEVASVGKREKEVDRERVNIYKFPVSANGRARTMEEKLGFAKVIEEKETGKVLGVTIVSANATDMIMEGVLGVKYEMTTEKIAEAIHPHPTLTETLLGAFEGKWAIHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEQ1</accession>
    <name>B7IEQ1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_57</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579321.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_57"/>
    <dbReference type="EnsemblBacteria" id="ACJ74565">
        <property type="protein sequence ID" value="ACJ74565"/>
        <property type="gene ID" value="THA_57"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_57"/>
    <dbReference type="eggNOG" id="COG2014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076326_1_1_0"/>
    <dbReference type="OMA" id="GICFTPI"/>
    <dbReference type="OrthoDB" id="1339731at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007161">
        <property type="entry name" value="DUF364"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025251">
        <property type="entry name" value="DUF4213"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04016">
        <property type="entry name" value="DUF364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13938">
        <property type="entry name" value="DUF4213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4213" evidence="2">
        <location>
            <begin position="11"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="DUF364" evidence="1">
        <location>
            <begin position="123"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="261" mass="28700" checksum="A0CB1AEA94B2141D" modified="2009-02-10" version="1">MIILTFSKKLYDEALKYINNETLKDYIIGLGLSAALLSDGRCGVSYTLREDTLGKCEEFFKCTGDFGNPVSKINPGMRVKEVLNIGLFSPDPLIRSVAYATLNAVFSTNEIKSMYSLGDLSQYINVNLDDTVGFIGRIDPLINLWKPKAWDILIFDRNRKGSDEILPDWAIVDLLPKCSVVVISGSAIVNGSIDWILNYVKTDKVAIVGPSTTLVPNVFPVKLLAGINVLNSDKLFKLISHGAGTKKIIAEKAVEKVVLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IET5</accession>
    <name>B7IET5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Histidine ammonia-lyase</fullName>
            <ecNumber evidence="3 6">4.3.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hutH</name>
        <name evidence="8" type="ordered locus">THA_91</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>L-histidine = NH4(+) + trans-urocanate</text>
            <dbReference type="Rhea" id="RHEA:21232"/>
            <dbReference type="ChEBI" id="CHEBI:17771"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="EC" id="4.3.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PAL/histidase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.3" evidence="3 6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103460.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_91"/>
    <dbReference type="EnsemblBacteria" id="ACJ74599">
        <property type="protein sequence ID" value="ACJ74599"/>
        <property type="gene ID" value="THA_91"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_91"/>
    <dbReference type="eggNOG" id="COG2986">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014801_4_0_0"/>
    <dbReference type="OMA" id="CAPQVAG"/>
    <dbReference type="OrthoDB" id="715502at2"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00549"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004397">
        <property type="term" value="F:histidine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00332">
        <property type="entry name" value="PAL-HAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005921">
        <property type="entry name" value="HutH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022313">
        <property type="entry name" value="Phe/His_NH3-lyase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="PTHR10362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01225">
        <property type="entry name" value="hutH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00488">
        <property type="entry name" value="PAL_HISTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0369">Histidine metabolism</keyword>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="494" mass="54838" checksum="BDC1D671E8E5C5F8" modified="2009-02-10" version="1">MIKIDGNNLRLDDIHNVARNFEKVDLDDFAKNKITESRNTIEKILSSGKTVYGINTGFGALVNVKISEKELYELQKNIVLSHSAGIGDPLDEDIVRAMMLLRANSLAKGFSGVRVEVIEKLLEFLNKKVYPYVPEKGSVGASGDLAPLSHIAMALIGEGYVIYNGKKEPTKKVLSELNINPIKLKEKEGLSLLNGTQYITSILALTIRDSIKLLDIATLIAAASVDVLLGTPAAFDERLQKARNHDGQIYIASLLRNYLNGSQIRESHKNCNKVQDAYTLRAIPQVYGAVYDTLIYAKKVAENEINAATDNPLVFDNDVISGGNFHGEPVALVSDFLAIALTDLGNMIERRIDRLVNPLINQKLPPFLASGKEGLNSGYMIWQYTAAAICNENKILSHPASTDTIPTSAYQEDHVSMGATSARKLRKIFFNIIDLVTIEAMLVKVALEFRKPLKSSKSIEEFFRKFDFIKINGDRYFGDDFENVKNIIIKEVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IET7</accession>
    <name>B7IET7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_93</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103470.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_93"/>
    <dbReference type="EnsemblBacteria" id="ACJ74601">
        <property type="protein sequence ID" value="ACJ74601"/>
        <property type="gene ID" value="THA_93"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_93"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_699935_0_0_0"/>
    <dbReference type="OMA" id="GTFQGNQ"/>
    <dbReference type="OrthoDB" id="554219at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001119">
        <property type="entry name" value="SLH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00395">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51272">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002854674" evidence="3">
        <location>
            <begin position="19"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="4">
        <location>
            <begin position="16"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="280"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="365" mass="40476" checksum="280553937952A28C" modified="2009-02-10" version="1">MKKVLIVITLLLSFLTFASFKDVPVNHWAYDAVNELSKLGIISGMPDGTFQGNQAMTRYQVAVAIYRTMNYLQEKIDKAVSNTSNISSLREQILTLSDIVSTAMNKIEDLATIKDTVQVLSSDLSELKTSIVNTKNDVKSLSIDLSSIKNSVADNSRKLEDLEAALSGNNLKTILDSKVSFNEYNAFKDSINSQFSNFQATMENKISGLEGEISVIKTDLETLKEKIAVIDSINDKVTGLEEYINAKTKAWDTRISALSGDITQLKVDFQTYTSQNDVKISQFEKQINSLNEKVNSMEQTVAKIETIESDFNQFKDDTNNSVEKLTQRVENLENSSNSTLSTFSMILSVISVAVSGVAIYLILSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEV4</accession>
    <name>B7IEV4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579353.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_110"/>
    <dbReference type="EnsemblBacteria" id="ACJ74618">
        <property type="protein sequence ID" value="ACJ74618"/>
        <property type="gene ID" value="THA_110"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_110"/>
    <dbReference type="eggNOG" id="ENOG5033K5D">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1884808_0_0_0"/>
    <dbReference type="OMA" id="FHYTIEM"/>
    <dbReference type="OrthoDB" id="1807343at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="135" mass="15385" checksum="587C9EC905F7923E" modified="2009-02-10" version="1">MPFHYTIEMLVNEPKAKSILQNEEIINLAEKNETKIEYQNGNLILTCAENQDLDEELVKKILSTISGSVTAKAYLIDGKSKIEIFNGKLDPKNPFGNSQDDIQERTIESIEEEIDDSGIVIDEDLEDFLDYDEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEY5</accession>
    <name>B7IEY5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dephospho-CoA kinase</fullName>
            <ecNumber evidence="1">2.7.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dephosphocoenzyme A kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">coaE</name>
        <name evidence="2" type="ordered locus">THA_141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3'-dephospho-CoA + ATP = ADP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18245"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoaE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.24" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103579.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_141"/>
    <dbReference type="EnsemblBacteria" id="ACJ74649">
        <property type="protein sequence ID" value="ACJ74649"/>
        <property type="gene ID" value="THA_141"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_141"/>
    <dbReference type="eggNOG" id="COG0237">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057180_2_2_0"/>
    <dbReference type="OMA" id="DVDKEYH"/>
    <dbReference type="OrthoDB" id="1515383at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00356"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00152">
        <property type="entry name" value="TIGR00152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="179" mass="20948" checksum="D2EB9F8ACC019D83" modified="2009-02-10" version="1">MKLILCVTGKIGTGKSTVSKFFKEKGFEYINMDELGKIAFEYEKDKILEVFNTLERNKIRDIVFQDQKMLKKLEKILHPKMLEILEQMTAKENYYAIEAAIKRRLKIKCDFTITVKCSLDTILKRVSERGLSQKDIENILKNQMDILDEGIIINNDGTIKELYEKLEKIYTFIMKSASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEZ9</accession>
    <name>B7IEZ9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chain P, L-Fuculose-1-Phosphate Aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579378.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_156"/>
    <dbReference type="EnsemblBacteria" id="ACJ74663">
        <property type="protein sequence ID" value="ACJ74663"/>
        <property type="gene ID" value="THA_156"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_156"/>
    <dbReference type="eggNOG" id="COG0235">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006033_3_0_0"/>
    <dbReference type="OMA" id="YATFGTH"/>
    <dbReference type="OrthoDB" id="599627at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldolase_II" evidence="1">
        <location>
            <begin position="3"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="212" mass="23561" checksum="0A783E3C57C6A530" modified="2009-02-10" version="1">MKKKILEASKFIVENGLVKGTWGNISIRYEDKIYITPSGIPYDILTEDMISVVDLRGNHLEGLKPSSELPTHLEIYRTREDVNAIVHTHPVFSTTVSLVADYIPPLIEDSVMILGSKILVAEYALAGTEDLAINVVKALGNNNAVLLKNHGLITVGVDIKEALTASLVCEKTAEIYLYALNIGKFSTLSEEDAIMLRNKYLNSYRQIEKDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF01</accession>
    <name>B7IF01_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Holo-[acyl-carrier-protein] synthase</fullName>
            <shortName evidence="13">Holo-ACP synthase</shortName>
            <ecNumber evidence="13">2.7.8.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">4'-phosphopantetheinyl transferase AcpS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">acpS</name>
        <name evidence="15" type="ordered locus">THA_158</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>apo-[ACP] + CoA = adenosine 3',5'-bisphosphate + H(+) + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="EC" id="2.7.8.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the P-Pant transferase superfamily. AcpS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.7" evidence="13"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579379.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_158"/>
    <dbReference type="EnsemblBacteria" id="ACJ74665">
        <property type="protein sequence ID" value="ACJ74665"/>
        <property type="gene ID" value="THA_158"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_158"/>
    <dbReference type="eggNOG" id="COG0736">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1000219_0_0_0"/>
    <dbReference type="OMA" id="ILFYGNL"/>
    <dbReference type="OrthoDB" id="1953248at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00483">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00101">
        <property type="entry name" value="AcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002582">
        <property type="entry name" value="ACPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000550">
        <property type="entry name" value="Hppk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01288">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55083">
        <property type="entry name" value="SSF55083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="SSF56214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00516">
        <property type="entry name" value="acpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01498">
        <property type="entry name" value="folK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00794">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 13" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="10" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HPPK" evidence="14">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ACJ74665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="272" mass="31867" checksum="1635AA6CEC9D15BE" modified="2009-02-10" version="1">MIVGLGNDIVEINRFDLKLVDRILTDIEKENKKLNAQYLAGRFSLKESFFKAIGTGISNNSFKDISFLNNKNGKPYFVMHKDFKGFNFCHIALSHDKFANSMVVLEKIKGKIFLGLGSNIGNREENLKKALEYIENFGINILRVSSIYITKPYGYLEQGDFFNIAVEVDSDLSPFELLNTIMEIERIMKRKREIKWGPRNIDIDILFYGNLVVENENLKIPHYDFKNRDFFIAPMYEIAKDFVDPIFDMSMNEYYSKLEKNWEVINWQMKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF19</accession>
    <name>B7IF19_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DegT/DnrJ/EryC1/StrS aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_178</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579393.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_178"/>
    <dbReference type="EnsemblBacteria" id="ACJ74683">
        <property type="protein sequence ID" value="ACJ74683"/>
        <property type="gene ID" value="THA_178"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_178"/>
    <dbReference type="eggNOG" id="COG0399">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033332_7_2_0"/>
    <dbReference type="OMA" id="PWPSFTQ"/>
    <dbReference type="OrthoDB" id="1722208at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="PTHR30244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="377" mass="42797" checksum="C0CFA428C3203692" modified="2009-02-10" version="1">MKIPLSNASISKEDIDEVLNVLKSGRLALGPYLEKFEDIVAKYIGIKYAVAVNSGTSALHLILKSLSFQEGDIILVPSFTFIASANVALFEKGIPVFIDIEKDTGNIDPDALEEFMEMVYSGKTKYDPKKVKFLMAVDIFGHPLNWEKIIKIAEKYNIQIIEDSCEALGSEYKGKKCGTFGKAGAFAFYPNKQITTGEGGIIVTNDEEIAHLSRAMRNQGRTNNGWLSHEIIGYNYRLDEMSAALGYSQMKKIDHIIEKRNKVAMYYNKILDFVETPTIKDYVTKMSWFVYVIKLPEGVDINKVIKYLSENGIESRNYFSPVHLQPLYRNLGYKKGMLPITEELSKRTLAIPFYTDLKKDEQDKVAYYLKKSIELFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF41</accession>
    <name>B7IF41_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_200</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YajC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579408.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_200"/>
    <dbReference type="EnsemblBacteria" id="ACJ74705">
        <property type="protein sequence ID" value="ACJ74705"/>
        <property type="gene ID" value="THA_200"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_200"/>
    <dbReference type="eggNOG" id="COG1862">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116157_2_0_0"/>
    <dbReference type="OMA" id="GMEMIIM"/>
    <dbReference type="OrthoDB" id="2082059at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003849">
        <property type="entry name" value="Preprotein_translocase_YajC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33909">
        <property type="entry name" value="PTHR33909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02699">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01853">
        <property type="entry name" value="YAJCTRNLCASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01323">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00739">
        <property type="entry name" value="yajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="130" mass="14351" checksum="4F5B960296B02BA1" modified="2009-02-10" version="1">MANIVYSGVPDPSASTGGATAAAPSAAGELFQMLFLLLIFFVMMYFLVILPQRKREKEFKKMISEIKRGDTIITSGGIVGKVIDVKKDTLKIKTSNTTELEVAKVYISKVLKNKDKTEENSKSSKEDNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF76</accession>
    <name>B7IF76_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">DNA repair protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">radA</name>
        <name evidence="8" type="ordered locus">THA_237</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Plays a role in repairing double-strand DNA breaks, probably involving stabilizing or processing branched DNA or blocked replication forks.</text>
    </comment>
    <comment type="domain">
        <text evidence="5">The middle region has homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the RecA family. RadA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579433.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_237"/>
    <dbReference type="EnsemblBacteria" id="ACJ74740">
        <property type="protein sequence ID" value="ACJ74740"/>
        <property type="gene ID" value="THA_237"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_237"/>
    <dbReference type="eggNOG" id="COG1066">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018264_0_1_0"/>
    <dbReference type="OMA" id="FELMRLA"/>
    <dbReference type="OrthoDB" id="505485at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01498">
        <property type="entry name" value="RadA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004504">
        <property type="entry name" value="DNA_repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041166">
        <property type="entry name" value="Rubredoxin_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18073">
        <property type="entry name" value="Rubredoxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00416">
        <property type="entry name" value="sms"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0346">Stress response</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RECA_2" evidence="7">
        <location>
            <begin position="63"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Lon-protease-like" evidence="5">
        <location>
            <begin position="343"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RadA KNRFG motif" evidence="5">
        <location>
            <begin position="248"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="92"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="446" mass="49084" checksum="C07F0D05F755A6E0" modified="2009-02-10" version="1">MTKKKTVYVCDNCGYESLKWFGKCPSCGAWDSAKEIKVSEDEKKNAGLSSRIFLLDDSLKIDENKRLKSNDPEIDKLFGGGIVSGQVILLGGEPGVGKSTLSLQLCKMLSKHGKVLYVSGEESIEQIGLRSKRLNISDSNLYVTTENEIESIFVAINQINPSFIVFDSIQTIFSNSVDGVPGGILQIKTVVEKIRKLSKEKGIPSLLVAHVNKEGNIAGPKLVEHVVDTVIYFEGEKNTDFRILRVLKNRFGPSGELAIFQMLEEGLIPLKDRVFIEESNMPGNVISCVFEGTRPILVQIQSLVSRDKIATARRIAHGIDVRKLIILGAVIAKHLNLPIDSHDLYLNVSGGLKITDPASDLAIASSILSSLYNTFLGKVVIIGEVGLDGSVRRVRNIKKRIENAKKSGYDRFIIPNVEDINGENIVKVSSLKQLNRIILGSKEELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF91</accession>
    <name>B7IF91_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">1-deoxy-D-xylulose 5-phosphate reductoisomerase</fullName>
            <shortName evidence="7">DXP reductoisomerase</shortName>
            <ecNumber evidence="7">1.1.1.267</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">1-deoxyxylulose-5-phosphate reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">2-C-methyl-D-erythritol 4-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dxr</name>
        <name evidence="11" type="ordered locus">THA_253</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13717"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:58262"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.267"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DXR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.267" evidence="7"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579445.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_253"/>
    <dbReference type="EnsemblBacteria" id="ACJ74755">
        <property type="protein sequence ID" value="ACJ74755"/>
        <property type="gene ID" value="THA_253"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_253"/>
    <dbReference type="eggNOG" id="COG0743">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035714_0_0_0"/>
    <dbReference type="OMA" id="FIDGSVM"/>
    <dbReference type="OrthoDB" id="1200722at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00092"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030604">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00183">
        <property type="entry name" value="DXP_reductoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003821">
        <property type="entry name" value="DXP_reductoisomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013644">
        <property type="entry name" value="DXP_reductoisomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013512">
        <property type="entry name" value="DXP_reductoisomerase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026877">
        <property type="entry name" value="DXPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036169">
        <property type="entry name" value="DXPR_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30525">
        <property type="entry name" value="PTHR30525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08436">
        <property type="entry name" value="DXP_redisom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02670">
        <property type="entry name" value="DXP_reductoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13288">
        <property type="entry name" value="DXPR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006205">
        <property type="entry name" value="Dxp_reductismrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69055">
        <property type="entry name" value="SSF69055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0521">NADP</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DXP_reductoisom" evidence="8">
        <location>
            <begin position="6"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="DXP_redisom_C" evidence="9">
        <location>
            <begin position="127"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="DXPR_C" evidence="10">
        <location>
            <begin position="235"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ74755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="353" mass="39725" checksum="418901C841F91B88" modified="2009-02-10" version="1">MEEKTVVILGATGSIGMQTLEVIKKLDNFRVKGIAFGKNTTLAEKIIKDFKVEYFYSPVKYNVSSIKDMVEKIKPDIVVAAIPGFDGVLATIDSIPYTKRLALATKEALVCASPFVKKACKENNVELIPVDSEHSAIFQIFDESVVKILITASGGAIRDKENIDDLSVEDILNHPVWSMGKKITVDSSTMVNKAFEVIEAFELFELENIEVKIHPTGTVHGAVFLKDGTVKIHFGFPDMKIPISYSLSYPKRLYENKNWPNFERKNFIFEDVNPSKYPAFFYGKSIIKDLAKRIAFNAADEIAVEKFLNGQIKFSKIADIVINTCEKINGNINDFKEIFEIDKIARQIAKEQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFB2</accession>
    <name>B7IFB2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amino acid racemase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579463.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_276"/>
    <dbReference type="EnsemblBacteria" id="ACJ74776">
        <property type="protein sequence ID" value="ACJ74776"/>
        <property type="gene ID" value="THA_276"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_276"/>
    <dbReference type="eggNOG" id="COG3457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067103_0_0_0"/>
    <dbReference type="OMA" id="VGCFGGV"/>
    <dbReference type="OrthoDB" id="859043at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ala_racemase_N" evidence="1">
        <location>
            <begin position="8"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="358" mass="39967" checksum="872A6BC8620C3447" modified="2009-02-10" version="1">MDFPKLFVNLKKIRENAEKLLETLSKLNIELVAVTKLTLGDPIIAKTLKDAGVIKLGESRLKNIKRMIENNVPGPFQLLRIPMPSELEDAVLLTDEILISEEKVAYMIDEIAKKYDKNIELIYMIDVGDLREGIWYEKAAYTIEKVAKKLKMAKIVGIGTNVGCFGGVLPTKENLNILVEIKDYLDGKLNTNLKISGGSTVTLSLIENNELPSKINQFRIGEGILLGTDATGNRDIPYLSQDTIILEAEVVEVDYKPSVPVGEVGRDSMGRIPHFEDLGWRNRIILAIGEQDIDSSGLKPFDEKLKVLHASSDHTIIDYTDSNKKYSIGDTLKFHLSYGAALRAFTSPFVKKEYIKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFI3</accession>
    <name>B7IFI3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ornithine carbamoyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_348</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the aspartate/ornithine carbamoyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579515.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_348"/>
    <dbReference type="EnsemblBacteria" id="ACJ74847">
        <property type="protein sequence ID" value="ACJ74847"/>
        <property type="gene ID" value="THA_348"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_348"/>
    <dbReference type="eggNOG" id="COG0078">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_3_3_0"/>
    <dbReference type="OMA" id="VYVKNWS"/>
    <dbReference type="OrthoDB" id="988597at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="OTCace_N" evidence="4">
        <location>
            <begin position="8"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="OTCace" evidence="3">
        <location>
            <begin position="160"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="329" mass="37277" checksum="52BF9310E07943A3" modified="2009-02-10" version="1">MSTFFRGRHFITTQDFTNEEIELMLDLARDLKIKFSHGEPTPYLLYKTLFLIFFDESTRTRNSMQAGIAQLGGTGIFLTPDKMQIAHGEVAKDTGIILSRFGDGIGIRYCKFGEGNKYLNEIAKHSKAPVMNLQDDVYHPFQVMADLMTIQERFGKNLRGLKVGISWAYAESHLKPLSVPQSQILLFTRFGMDVTLAYPEGFDLMPDIVEQAKKNAEMYGGKLEISHKMEDAFVDADIVIPKNWGGFFVSDNPDEIRAEQAKHKNWICTEELMKLTKKHSIYMHALPADRGKEVVDSVIDGPHSVVYDEAENRLHTAKAVMTLLMGGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFJ0</accession>
    <name>B7IFJ0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_360"/>
    <dbReference type="EnsemblBacteria" id="ACJ74854">
        <property type="protein sequence ID" value="ACJ74854"/>
        <property type="gene ID" value="THA_360"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_360"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_369161_0_0_0"/>
    <dbReference type="OMA" id="ICEDEYG"/>
    <dbReference type="OrthoDB" id="628728at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="754" mass="86601" checksum="3D54538F39CFF081" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFL0</accession>
    <name>B7IFL0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Damage-inducible protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579536.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_381"/>
    <dbReference type="EnsemblBacteria" id="ACJ74874">
        <property type="protein sequence ID" value="ACJ74874"/>
        <property type="gene ID" value="THA_381"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_381"/>
    <dbReference type="eggNOG" id="COG0534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_5_3_0"/>
    <dbReference type="OMA" id="AAWFELF"/>
    <dbReference type="OrthoDB" id="1415498at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="467" mass="51898" checksum="22B2D9FE01D4BE29" modified="2009-02-10" version="1">MENKHRVDIFNRGIFSSLFLLSWPLIISNLMQTFYNAVDGYFLGKLGKIEFSAPTIVWPIIFVFISLSIGFSQAGVTLVAQFTGAKDKKLAQKSAGQSLLTSTLIGVIFSIFSIIISRPIIMAVAGKESAEVVDYAVKYFNIIMLGLPFGFIFNTVSSIIRGWGDSKFTMNLMLISTIINIILDPLLIFGIGVFPKMGVVGAALATTIARIIAALMAIESLFKGKRGFKIHLRDLTPDWQLIKKVFRIALPSSLSMSITSLGFVFIMRFVSSFGPTVVSAYGVGNRIINFITMISFGIANSVTAMVGQFLGAGDVKSAEKTLKVAFWTNFTIVFFLSTLTFFFGGQLTKFFINEKDVIEVGYIFFRYVSFSLPFFTSMSVFVHALVGAGRTDLSMIVDITRLWGIRVPLVAFFSTKYGFTGLFFAMIISNVSALLLAYLFIKFSNWKKPITRDLKRGNLDDIYRQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFL6</accession>
    <name>B7IFL6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 type transporter superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_387</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579541.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_387"/>
    <dbReference type="EnsemblBacteria" id="ACJ74880">
        <property type="protein sequence ID" value="ACJ74880"/>
        <property type="gene ID" value="THA_387"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_387"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_0_0_0"/>
    <dbReference type="OMA" id="RYGLFKR"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="6">
        <location>
            <begin position="132"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="330" mass="37952" checksum="9F430101F045C5E5" modified="2009-02-10" version="1">MKILMLTKSLFLEYFRNKISFFFNILFPLVLFILFSLVFFSEDTANFAYFSNIELDIFGKSYNDEEKLLSDRGKYDAVAVIKDNEVIVYKNSNSAMVYVWIDTLVNKVKSFGLKNVVNIKETKLDYIIFSQRENIYIGTFALSIMSIGMFSVVNLFDRYKRVGTLKRLKLLPMSSLSFVFSFSISQFFISFISLVIIRLIGAFLFSISLKVNVFYFSIALVSSVIGMLGIGTLISVLFKKSAANVSQFLYIVFIFFSGIYFPLEFLPKFLKNLSFFIPVKYIVDIFKFSMGMQVINISKFFIYSFILIVFGLFLLLIGGKILFETEKVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFN6</accession>
    <name>B7IFN6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Outer membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_407</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579555.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_407"/>
    <dbReference type="EnsemblBacteria" id="ACJ74900">
        <property type="protein sequence ID" value="ACJ74900"/>
        <property type="gene ID" value="THA_407"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_407"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_699935_0_0_0"/>
    <dbReference type="OMA" id="DHWAYEY"/>
    <dbReference type="OrthoDB" id="554219at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001119">
        <property type="entry name" value="SLH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00395">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51272">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857355" evidence="3">
        <location>
            <begin position="20"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="4">
        <location>
            <begin position="17"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ74900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="342" mass="38145" checksum="C10F9C67927613B4" modified="2009-02-10" version="1">MKKLLVMLSVVLVSVFALAELKDVPTNHWAYDSVVMLEKAGVITGYPDGTFKGTNNVTRYELAVFLARIMKLLEEDIQKLSVTVDVHENDISALYDIVGQLKKSVALMAKSEDVEALSKRVDVLDKDIIRIYESLTTKASKEDVEQMLNGSLESVNGQIEFLYKKITLSDENGKKYTDEKINELEGKVLNIETNVNDAIPMLRNLVYQNSENIKNLESKLIRYINVKIKSVDSKLKALDEVKQMAEFNTDTLNGFAAKLGEVEYALRKQIEETNKVVEANSKEIEGLKTNVEEIKASVNEKASKTEVEDLSKKVNSVNTMSIIGLITGLVGLGLAIYAVFKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFN8</accession>
    <name>B7IFN8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_409</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in protein export. Participates in an early event of protein translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SecG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_409"/>
    <dbReference type="EnsemblBacteria" id="ACJ74902">
        <property type="protein sequence ID" value="ACJ74902"/>
        <property type="gene ID" value="THA_409"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_409"/>
    <dbReference type="eggNOG" id="COG1314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094156_6_2_0"/>
    <dbReference type="OMA" id="AYTMFGR"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004692">
        <property type="entry name" value="SecG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03840">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01651">
        <property type="entry name" value="SECGEXPORT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00810">
        <property type="entry name" value="secG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="54" mass="5674" checksum="2D6CFD01048C9206" modified="2009-02-10" version="1">MGTVMLVVHAIISVILIYLSLKQMGKFAELGGAFGSGSMNTIFGRDKGLDAEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFQ5</accession>
    <name>B7IFQ5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_426</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579571.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_426"/>
    <dbReference type="EnsemblBacteria" id="ACJ74919">
        <property type="protein sequence ID" value="ACJ74919"/>
        <property type="gene ID" value="THA_426"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_426"/>
    <dbReference type="eggNOG" id="ENOG502Z9AE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065107_0_0_0"/>
    <dbReference type="OMA" id="MIFVTSE"/>
    <dbReference type="OrthoDB" id="1482071at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="323" mass="36420" checksum="519790DCB054B447" modified="2009-02-10" version="1">MIKTFEKHKVIAIVGLEKNTGKTETFNFLLKNLNKRYTLGITSIGIDGEEKDQVTFTKKPNIFVEKNIIFATTEKYFKMKDFSAEILYVSDRRTSTGRIIIARARERGKIILAGPTTIDWLNEINNKLFNFGAKKILIDGALSRISSSSISDAVVLATGAAVSLDVEKIASKTIDLIYKINLPKYKLYNLTSINLEKGIYLKDLKLLGESTLNFEDFDKLKEVKEIIIIGALTDSFVKKLIDKNIILDIIVKDFSKIFAKSSTLKLFKKLGGNIFVLNKPNLCLITINPTSPYGYQINEEVLIEKISKEIDIPVVNIRKVDNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFR4</accession>
    <name>B7IFR4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_435</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579579.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_435"/>
    <dbReference type="EnsemblBacteria" id="ACJ74928">
        <property type="protein sequence ID" value="ACJ74928"/>
        <property type="gene ID" value="THA_435"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_435"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_828834_0_0_0"/>
    <dbReference type="OMA" id="STDYQIA"/>
    <dbReference type="OrthoDB" id="2146218at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857387" evidence="3">
        <location>
            <begin position="19"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="215"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ74928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="335" mass="39518" checksum="EFC61C797DF73916" modified="2009-02-10" version="1">MKKLVSFLVLFFVSFSFALSDDIILGLKQKDSYVSNALTFEKKKIEYENLKITADSTISKLSAEVQYYSALSQFQNGESNFFKSVVSILFSLNINKLNLQIKKLSYENAKISYENSKELYEKNLISKAEWLKAEYDLKNAEYNLKLAQKNFEDSLSDFRKYFDFDENFTLDVNFIDLKITTNVEELPDYKVQLLNYQILYDEYMVEKDYLSESEKRQKEIDLQISKINLEDLKESIEKSISSFNDELELIKKELEVLEANRKYQEENLNYTKVRYEKGLVEKSQYNQAQISYLNALLQIENKKMNYITKLIDISKYSERSIEDILKEALVINWGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFR7</accession>
    <name>B7IFR7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent zinc metalloprotease FtsH</fullName>
            <ecNumber evidence="6">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ftsH</name>
        <name evidence="9" type="ordered locus">THA_440</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the central section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104198.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_440"/>
    <dbReference type="MEROPS" id="M41.021"/>
    <dbReference type="EnsemblBacteria" id="ACJ74931">
        <property type="protein sequence ID" value="ACJ74931"/>
        <property type="gene ID" value="THA_440"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_440"/>
    <dbReference type="eggNOG" id="COG0465">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_16_2_0"/>
    <dbReference type="OMA" id="YDKQGGG"/>
    <dbReference type="OrthoDB" id="190468at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6 9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="8">
        <location>
            <begin position="192"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="423"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="200"/>
            <end position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="499"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="618" mass="68476" checksum="C1B6B6F4BE77C5DB" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFR8</accession>
    <name>TILS_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Ile)-lysidine synthase</fullName>
            <ecNumber evidence="1">6.3.4.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA(Ile)-2-lysyl-cytidine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(Ile)-lysidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tilS</name>
        <name type="ordered locus">THA_441</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP + diphosphate + H(+) + lysidine(34) in tRNA(Ile2)</text>
            <dbReference type="Rhea" id="RHEA:43744"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10670"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83665"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text>The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA(Ile)-lysidine synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.19" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579581.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFR8"/>
    <dbReference type="SMR" id="B7IFR8"/>
    <dbReference type="STRING" id="484019.THA_441"/>
    <dbReference type="EnsemblBacteria" id="ACJ74932">
        <property type="protein sequence ID" value="ACJ74932"/>
        <property type="gene ID" value="THA_441"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_441"/>
    <dbReference type="eggNOG" id="COG0037">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018869_0_1_0"/>
    <dbReference type="OMA" id="NRIRNHY"/>
    <dbReference type="OrthoDB" id="666797at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01992">
        <property type="entry name" value="PP-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01161">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012796">
        <property type="entry name" value="Lysidine-tRNA-synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012094">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012795">
        <property type="entry name" value="tRNA_Ile_lys_synt_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033">
        <property type="entry name" value="PTHR43033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11734">
        <property type="entry name" value="TilS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00977">
        <property type="entry name" value="TilS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02433">
        <property type="entry name" value="lysidine_TilS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02432">
        <property type="entry name" value="lysidine_TilS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000137882" description="tRNA(Ile)-lysidine synthase">
        <location>
            <begin position="1"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01161"/>
        </source>
    </evidence>
    <sequence length="427" mass="50205" checksum="347DE27F0C50E807" modified="2009-02-10" version="1">MLTLLEKFIQKIEEYNLIEENDRILVAVSGGVDSSVLLNLLARVQEYFKFSIFCATVDHGIRKESKEEVKFVYNMAQSLNVECFIKEFDVPLYAKENKLSIEEAARKLRYKFLNEIAKKINANKVATAHNLNDLAETVLFRLARGTGPFGIYGMKPRNGNIIRPLLFFERKEIEQFATENKIPFVVDKTNFDTKYTRNFIRHKIIPIFKEINPLFEQAVLRFVENVWELDSFVKDKLNVETFEFEGRIFFKVPKDEYILVEFIRRKTMEHFGRAPDKEKLDRLKKNLYKTSFKISFWGDYGVEISYGYGVLGKFIEHMNYECSMDCQNNVNFGPFVVKFGNNGIIFKKMNLTIRNYRFGDKTKGGKKLKEIFVEKKVPSFIRRIIPIFVDEDGYVFYIPNVFLDRDYLSKEENGISIKVEMKGGFWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFS7</accession>
    <name>B7IFS7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_450"/>
    <dbReference type="EnsemblBacteria" id="ACJ74941">
        <property type="protein sequence ID" value="ACJ74941"/>
        <property type="gene ID" value="THA_450"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_450"/>
    <dbReference type="eggNOG" id="ENOG5033MPI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_692125_0_0_0"/>
    <dbReference type="OMA" id="FGWRICQ"/>
    <dbReference type="OrthoDB" id="649843at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="393" mass="46529" checksum="8F3F7F077D9DDF94" modified="2009-02-10" version="1">MNNNDDKLKNILKLKEELEKDLIKKGKIKNKSEQKSRKVQDLEQMKIIKEKIIKEANLAADKTLSIFDINSQDYDSSVMDVIKTLRKFLINENSPEKRTLFLAILNILEGNFEKNKTLLENQTDVIFKYNYLLSRMYNREDVIKEIIQFVKNFPDSIYPYLLLLEYYLIEGNFSNFSKILSLTSKINNFFKIIYQVYTNTLEDVGIVKNAVLHKKFTELLLYIIKQKSLETENTKSYCLNVNYSKKEGTIPNPKEYCIKANFAQAAWSIVNNRKFDESKLKIFEKTPEYNLFYGFYYLNNHELENAKKYFEKFENQVENVSVKIIAKTISYIGMRQFYQNIKSNIFKEERGKILEVINNYPLHDFIVEYFDPEIVRLLFAEKHCCIVYGGNKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFW3</accession>
    <name>B7IFW3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">1-deoxy-D-xylulose-5-phosphate synthase</fullName>
            <ecNumber evidence="10">2.2.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">1-deoxyxylulose-5-phosphate synthase</fullName>
            <shortName evidence="10">DXP synthase</shortName>
            <shortName evidence="10">DXPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dxs</name>
        <name evidence="12" type="ordered locus">THA_486</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-glyceraldehyde 3-phosphate + H(+) + pyruvate = 1-deoxy-D-xylulose 5-phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:12605"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="10">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the transketolase family. DXPS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.7" evidence="10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_486"/>
    <dbReference type="EnsemblBacteria" id="ACJ74977">
        <property type="protein sequence ID" value="ACJ74977"/>
        <property type="gene ID" value="THA_486"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_486"/>
    <dbReference type="eggNOG" id="COG1154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_1_4_0"/>
    <dbReference type="OMA" id="QVGYHAQ"/>
    <dbReference type="UniPathway" id="UPA00064">
        <property type="reaction ID" value="UER00091"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008661">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052865">
        <property type="term" value="P:1-deoxy-D-xylulose 5-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02007">
        <property type="entry name" value="TPP_DXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00315">
        <property type="entry name" value="DXP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005477">
        <property type="entry name" value="Dxylulose-5-P_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322">
        <property type="entry name" value="PTHR43322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13292">
        <property type="entry name" value="DXP_synthase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00204">
        <property type="entry name" value="dxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="11">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Thiamine pyrophosphate binding" evidence="10">
        <location>
            <begin position="140"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ74977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="613" mass="69223" checksum="A08CA5DE199E9ACC" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG10</accession>
    <name>B7IG10_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Negative regulator of flagellin synthesis</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_536</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FlgM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579638.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_536"/>
    <dbReference type="EnsemblBacteria" id="ACJ75024">
        <property type="protein sequence ID" value="ACJ75024"/>
        <property type="gene ID" value="THA_536"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_536"/>
    <dbReference type="eggNOG" id="COG2747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169011_9_0_0"/>
    <dbReference type="OMA" id="INGPRDI"/>
    <dbReference type="OrthoDB" id="2030715at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035890">
        <property type="entry name" value="Anti-sigma-28_factor_FlgM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007412">
        <property type="entry name" value="FlgM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031316">
        <property type="entry name" value="FlgM_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04316">
        <property type="entry name" value="FlgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101498">
        <property type="entry name" value="SSF101498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03824">
        <property type="entry name" value="FlgM_jcvi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FlgM" evidence="7">
        <location>
            <begin position="31"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="85" mass="9549" checksum="3EEB7681145D3D8E" modified="2009-02-10" version="1">MEIKKIGGYGYIQGINSKNAEKINRKKNGKDKVDFSATLEIKKLLYESKNIPEVREKLVNELKQAIENGTFKIDPEKIAKAILGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG20</accession>
    <name>PURA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenylosuccinate synthetase</fullName>
            <shortName evidence="1">AMPSase</shortName>
            <shortName evidence="1">AdSS</shortName>
            <ecNumber evidence="1">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purA</name>
        <name type="ordered locus">THA_546</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579642.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IG20"/>
    <dbReference type="SMR" id="B7IG20"/>
    <dbReference type="STRING" id="484019.THA_546"/>
    <dbReference type="EnsemblBacteria" id="ACJ75034">
        <property type="protein sequence ID" value="ACJ75034"/>
        <property type="gene ID" value="THA_546"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_546"/>
    <dbReference type="eggNOG" id="COG0104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029848_0_0_0"/>
    <dbReference type="OMA" id="FHHAKPI"/>
    <dbReference type="OrthoDB" id="232152at2"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="PTHR11846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000194777" description="Adenylosuccinate synthetase">
        <location>
            <begin position="1"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="37"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="39"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="286"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="318"/>
            <end position="320"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="390"/>
            <end position="392"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <sequence length="401" mass="45185" checksum="D5D447D78FB926FA" modified="2009-02-10" version="1">MKSVIFGLQWGDEGKGKVTTYFSKDYDYVVRYSGGSNAGHTVEYTDFKLIHHLLPSFYVKKNVGAIISNGVVLDLEQLVEEIEEFKSKTGTYPKLYISELAHVVLPHHKKLDEKLEQIKGKNAVGTTKRGIGPAYADKVHRIGIRLSDFKNKDKFYEKIKNISKLYKNLYNIEVESIENVLTSYEKLKSHIVPHGEIINLINQNKILFESTQGVLLDIDVGTYPYVTGANCNTTGIQNGVGFPVKTENYIGVFKAYLTRVGNGPFPTEAFGKEGEEIRKRGHEFGATTGRPRRCGWLDLPLLKYAITVSGATELVMTKGDILNGMEKIKVCVAYKIDGNIVDRISSVDDLEKAEPIYETLDGWENHTSKEFNEYLSFIESYVKRKITHISVGPKVEEIIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG35</accession>
    <name>B7IG35_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Xaa-Pro dipeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_562</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579650.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_562"/>
    <dbReference type="MEROPS" id="M24.031"/>
    <dbReference type="EnsemblBacteria" id="ACJ75049">
        <property type="protein sequence ID" value="ACJ75049"/>
        <property type="gene ID" value="THA_562"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_562"/>
    <dbReference type="eggNOG" id="COG0006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_4_2_0"/>
    <dbReference type="OMA" id="YCSDRTR"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase_N" evidence="5">
        <location>
            <begin position="3"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24" evidence="4">
        <location>
            <begin position="137"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="356" mass="39818" checksum="6BE6045DF86ACDC5" modified="2009-02-10" version="1">MNLVNLLNEKGLDTLLVLNFEGSNKPTTRFISGFSGSFSGVIFSKDKRIIVTDSRYWEQVKLESEFDLVKFKGDKKFLDLIVELLKDIKAKKVGIEKDKTSAKVMEYLNNNLDGVEFIDVSQELLRLRAVKTDEEIELIRKAIHIAEEAFKKTLEIVKVGMTEKEFAAYLEYQIKMLGGDKFSFDTIVASGWRGSLPHGIASEKAIEKGEPVVVDWGAFYKGYASDLTRVFCIGEPSEEVKKVHSVVYKAQEKAIEIARASLTGAEIDLAAREHIKNEGYGGYFGHSLGHGIGLEVHEEPRLSYLNSEKLPANSVVTVEPGIYLPNKFGIRIEDDILLTESGCEVLSSLPRDIFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG70</accession>
    <name>B7IG70_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_597</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_597"/>
    <dbReference type="EnsemblBacteria" id="ACJ75084">
        <property type="protein sequence ID" value="ACJ75084"/>
        <property type="gene ID" value="THA_597"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_597"/>
    <dbReference type="eggNOG" id="COG1373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041527_2_0_0"/>
    <dbReference type="OMA" id="AVFKHIY"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="48"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="396" mass="46789" checksum="8986685A6622166B" modified="2009-02-10" version="1">MNFDDLYIMNPWWKLKNEVYNDKHIVSFEKSKFKYYPEKLFREIDLSNPGIYTLRGPRQIGKTTFLKLLTKKLIENNTNPLNIFYLTCDGLKGRHELTEILKVYFQTYNTSKKDMKYIFLDEVTSIEDWQYSIKYLVDIGILDNSLLILTGSSAYDLKSSSERLPGRKGYGKDLVYLPITFREFLKSLNVEIEKKDIEEILSSTEEELKTLQFKYSFIQEYFLKYVNCGGFPKIIDEFLNTGTISELTRKIYKDFVLGDAEKYIKSRTNIIEIFKKIPDIIGRRFSWNSLKEHLNGLFESVDTVQKYFGYFGYSFIFSTVFFVDIYKKTIKPKKQKKIYPLDNIVNLVIYDLTGKEIGLPQRIEMLTLNHILKNEDVVDNGLNLYHGPFFLVFRSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG94</accession>
    <name>B7IG94_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_622</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579690.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_622"/>
    <dbReference type="EnsemblBacteria" id="ACJ75108">
        <property type="protein sequence ID" value="ACJ75108"/>
        <property type="gene ID" value="THA_622"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_622"/>
    <dbReference type="eggNOG" id="COG0733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006855_3_3_0"/>
    <dbReference type="OMA" id="GMATFIF"/>
    <dbReference type="OrthoDB" id="376209at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="PTHR11616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SSF161070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0769">Symport</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="357"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="505" mass="55327" checksum="474860AA910EADCD" modified="2009-02-10" version="1">MARQKWGSRWAFVLAAVGSAAGLGNAWRFPYMAYSNGGGAFYVPYFIALFLVGIPLLMAEFAIGQGLQSSAPKSMAIIRKNAEFIGWFAVIGGAIITFYYNVIMAYIFNYLYYSFGVAWKDDPNGFFFGKFLQVSSGPGELGSIRWPIVIGLALTWLWIYFILRKGTTSVGKTVAWTVPLPVVLLLILGIRGITLDGAAKGLNFLFEPNFAKLADPRVWANAFGQIFFTLSLAFGIMIAYGSYNKKEEDIANNAIITALGNSATSYLAGIAVFSVLGYMATQLSVPVDKVVSGGIGLAFVVYPQAISLFPGGVVVQSIIGLLFFLMLLTLGIDSAFSLVEAVEAAASDKFKVNKKAFLIGFSIFGFLAGLLFSTQGGLYWLDIIDHFVSVYGLLIAGILESIIIGWLFGAEKLRKYINEVSEIKIGKWFNFSIKFLIPAVLIIILGLNFFDELKKPYGDYPSWALWTGFSMLIITPIIAIILSKIPPKDKDYYKKVEIDLTEGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGA1</accession>
    <name>B7IGA1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Transcription termination/antitermination protein NusG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">nusG</name>
        <name evidence="9" type="ordered locus">THA_632</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Participates in transcription elongation, termination and antitermination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the NusG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104462.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_632"/>
    <dbReference type="EnsemblBacteria" id="ACJ75115">
        <property type="protein sequence ID" value="ACJ75115"/>
        <property type="gene ID" value="THA_632"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_632"/>
    <dbReference type="eggNOG" id="COG0250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_778296_0_0_0"/>
    <dbReference type="OMA" id="MIMNDEF"/>
    <dbReference type="OrthoDB" id="2027986at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00948">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043425">
        <property type="entry name" value="NusG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040473">
        <property type="entry name" value="NusG_add"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001062">
        <property type="entry name" value="Transcrpt_antiterm_NusG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015869">
        <property type="entry name" value="Transcrpt_antiterm_NusG_bac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265">
        <property type="entry name" value="PTHR30265"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265:SF2">
        <property type="entry name" value="PTHR30265:SF2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02357">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18298">
        <property type="entry name" value="NusG_add"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00338">
        <property type="entry name" value="NUSGTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82679">
        <property type="entry name" value="SSF82679"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01014">
        <property type="entry name" value="NUSG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5 6" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="3 5 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 5 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="NGN" evidence="7">
        <location>
            <begin position="2"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="8">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="354" mass="39604" checksum="448AC73497D529DC" modified="2009-02-10" version="1">MKKRWYILQTLAGYEATAKENLEAKIKAQGLEHVISRVLLPEEVVIDASSKSSERHIVSLNAKILVSNGILVKKGDLLAEEPAIRVRRDGIVTDVRNAKKVVIETEDKKFTKTYVIPQKNKPITGLKIGGHVKQGMPLSSDEEIICEIDGIIIQTENMKRIVVRNSISEIDVYVVPNETFVSTIKKGTQVKTGQILAEPKKVTAKSSGRIEIIDFPTKKEIKVQKVKVRKLFPGYLFVEMIMNDEFWHFVRTVPGIIDFVSSGGRPIPINDKEAKVLLRLAGIEEAPQVEKEKEIKIEFDFEVGDSVKIVSGPFEGFVGSVKEINPEHNELKVSVTIFGRETPVTVHTSEVEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGC8</accession>
    <name>B7IGC8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">OB-fold nucleic acid binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_665</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579711.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_665"/>
    <dbReference type="EnsemblBacteria" id="ACJ75142">
        <property type="protein sequence ID" value="ACJ75142"/>
        <property type="gene ID" value="THA_665"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_665"/>
    <dbReference type="eggNOG" id="COG2374">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_513798_0_0_0"/>
    <dbReference type="OMA" id="GLMIYAP"/>
    <dbReference type="OrthoDB" id="552803at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="530" mass="59407" checksum="64723D319176705D" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGD4</accession>
    <name>B7IGD4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, LacI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_673</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579717.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_673"/>
    <dbReference type="EnsemblBacteria" id="ACJ75148">
        <property type="protein sequence ID" value="ACJ75148"/>
        <property type="gene ID" value="THA_673"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_673"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_0"/>
    <dbReference type="OMA" id="HSEFVFM"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00036">
        <property type="entry name" value="HTHLACI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="4"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="330" mass="37465" checksum="F21078DF9C1BDFFB" modified="2009-02-10" version="1">MNRPSIKEVAKKAGVSISTVSRVLNNSAPVSEELRKKVEAAVKELGYYPSNIAKSLRKGKTKTIGFILPDITNPFFADIVRGAEDFLRKKGYTLILCNSDQNGEQEIQLLETLISKHIDGLLFTGTGSFNLLLEEKIRKGLKVVFLDRIIQGINSSYVIVDNKKGMELLLDFLIKSGHKSFIFINGNKETFSAKIRYEVFVEKMEKAKLEYKHYFTNFSYEAGYNFAKKIRKLPHAIVCGNDLIAYGVIDAIEEKGYRVPDDVSVTGFDDILFSKHFKPPLTTIRQPKYKMGMKAAELLIKIIEENKFLNRGIILEPELIIRKSTKELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGH9</accession>
    <name>B7IGH9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferric uptake regulation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_725</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fur family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104652.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_725"/>
    <dbReference type="EnsemblBacteria" id="ACJ75193">
        <property type="protein sequence ID" value="ACJ75193"/>
        <property type="gene ID" value="THA_725"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_725"/>
    <dbReference type="eggNOG" id="COG0735">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_5_1_0"/>
    <dbReference type="OMA" id="DIHHLTC"/>
    <dbReference type="OrthoDB" id="1630127at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="126" mass="14917" checksum="D92B43FBBFC1FD15" modified="2009-02-10" version="1">MLTKWRKLVFDVINSSEKPLNAEDIYRLNNFKPNLSTVYRALNYLEKKSYISSVSFDGGTKYYFSKDKHFHFLYCTNCGKIEVFEECMASELEKKIKSKFGYLITDHVFYFKGLCKKCRKEVKGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGI0</accession>
    <name>B7IGI0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative periplasmic metal-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_726</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579754.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_726"/>
    <dbReference type="EnsemblBacteria" id="ACJ75194">
        <property type="protein sequence ID" value="ACJ75194"/>
        <property type="gene ID" value="THA_726"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_726"/>
    <dbReference type="eggNOG" id="COG0803">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016838_1_0_0"/>
    <dbReference type="OMA" id="WLDPFFL"/>
    <dbReference type="OrthoDB" id="1703187at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006127">
        <property type="entry name" value="ZnuA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01297">
        <property type="entry name" value="ZnuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="263" mass="30683" checksum="FCA9BA25625FF2A4" modified="2009-02-10" version="1">MNNKILLTVFLIFVSSLSFSLIVTTINPYYLIVKQIVGEKDEVYLLLSPNVNPHTYSLRVSDVKILSKANIIFANGGIEPDLSKFYVVYLRDYIPSLFVEYNNPHFWLDPYFVKFYIIPTIVEKLSKVYPQYRSDFEANAKILLGEIDEFLRDYKALNLSGTVLVHHPSFYYFFKELNLKVKWVEQGHNVSVGMKKLIETIKSENIIAIFSEVQQSKDEIKIISKQLNKKYYTLDPLGVNAKKFIDIYYQNFEEIRKALNDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGJ0</accession>
    <name>B7IGJ0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Bifunctional purine biosynthesis protein PurH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579764.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_738"/>
    <dbReference type="EnsemblBacteria" id="ACJ75204">
        <property type="protein sequence ID" value="ACJ75204"/>
        <property type="gene ID" value="THA_738"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_738"/>
    <dbReference type="eggNOG" id="COG0138">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016316_1_0_0"/>
    <dbReference type="OMA" id="EQDRVGC"/>
    <dbReference type="OrthoDB" id="1049662at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="PTHR11692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="297" mass="32599" checksum="E41B5CD5886B81F1" modified="2009-02-10" version="1">MYLEKFDLDLRYGENSHEKAEIFGKPAFEILHEGKQLSYNNILDAEAAYVIANNLKKLKGYASVVVKHQTPCGASIKNDKIESILSAIAADEESSFGGILAVNFEFDEKCAMAIKKKYLEVVVAESFTQEAIEALSKKKVRILKINDYEPKVGKPAFGAFLVGERKLPDLKFTHVAGPQLDVEELNELLFAFIVVEGVKSNAILISKDMTTVGIGTGQPSRKRAAWIATELAGEKAKDAYVASDAFFPFPDGLEILAKAGVKAVVSPMGSIRDEEVINAAEKMGLSFYKAEGRVFRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGM2</accession>
    <name>B7IGM2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_773</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579789.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_773"/>
    <dbReference type="EnsemblBacteria" id="ACJ75236">
        <property type="protein sequence ID" value="ACJ75236"/>
        <property type="gene ID" value="THA_773"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_773"/>
    <dbReference type="eggNOG" id="COG4608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_0"/>
    <dbReference type="OMA" id="PEIDRKW"/>
    <dbReference type="OrthoDB" id="1303270at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="2"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="305" mass="35114" checksum="C5C8458A803AA2F4" modified="2009-02-10" version="1">MIEVTNLTKAYNIGSFGKEKFYAVDNVSFKIDDNQIVSLIGESGSGKTTIGKLILKLIKPTSGEILFQGKNIKNLKNKEYYRYVQGVFQDPFSSYNPIFKINRVFDMVREEFYPKTDKDKWNEKVEKVISEVGLNPKEILGKFPHQLSGGQLQRLLIARTLLMETKFLVADEIISMLDASTRIDILNILIDLKEKGLSVLFITHDLSLGYYTSDKTIILYRGSIMEYGDTEKVFNNPLHPYTIMLMNSVPTMNKKWTKEELIAESRRTPPGYCKFYDRCPLADKECKNVQLKKVEEEHYVACVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGP1</accession>
    <name>B7IGP1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MTA/SAH nucleosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_793</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100365.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_793"/>
    <dbReference type="EnsemblBacteria" id="ACJ75255">
        <property type="protein sequence ID" value="ACJ75255"/>
        <property type="gene ID" value="THA_793"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_793"/>
    <dbReference type="eggNOG" id="COG0775">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031248_2_1_0"/>
    <dbReference type="OMA" id="CASCVQL"/>
    <dbReference type="OrthoDB" id="1860206at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="1">
        <location>
            <begin position="30"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="217" mass="24227" checksum="A281DD68749F57F7" modified="2009-02-10" version="1">MIVITSVLREEIVGAFRELMPVLENGEILKRPYMRGLIGANEVLLVYGLIGKVESAMMAQALIDNFHPKYFIHCGGAGIINSERKIGDIVCGTEYIEHDIKFRQKQIKKIKASQTLIERISDVYNKVILGPIASGDIFVDSVEEKTRIFNETKAELVDMDSAAIAKVCYENDVEFCAIKIALDRSVEGSTIEIQQNLKKLAPFPSAIVAEMLEKHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGR8</accession>
    <name>B7IGR8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_820"/>
    <dbReference type="EnsemblBacteria" id="ACJ75282">
        <property type="protein sequence ID" value="ACJ75282"/>
        <property type="gene ID" value="THA_820"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_820"/>
    <dbReference type="HOGENOM" id="CLU_3223193_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="44" mass="4952" checksum="40538860A660135B" modified="2009-02-10" version="1">MQKIGGIVMKIFLKWSDIKVKALKITPSRINCSLSSAHCSYFAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGW6</accession>
    <name>B7IGW6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Glucose-1-phosphate thymidylyltransferase</fullName>
            <ecNumber evidence="4 5">2.7.7.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rfbA</name>
        <name evidence="7" type="ordered locus">THA_870</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 1-phosphate + dTTP + H(+) = diphosphate + dTDP-alpha-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:15225"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
            <dbReference type="ChEBI" id="CHEBI:57477"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.7.7.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the glucose-1-phosphate thymidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.24" evidence="4 5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104361.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_870"/>
    <dbReference type="EnsemblBacteria" id="ACJ75330">
        <property type="protein sequence ID" value="ACJ75330"/>
        <property type="gene ID" value="THA_870"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_870"/>
    <dbReference type="eggNOG" id="COG1209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_9_0_0"/>
    <dbReference type="OMA" id="PFIMYLG"/>
    <dbReference type="OrthoDB" id="1004719at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008879">
        <property type="term" value="F:glucose-1-phosphate thymidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045226">
        <property type="term" value="P:extracellular polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02538">
        <property type="entry name" value="G1P_TT_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005907">
        <property type="entry name" value="G1P_thy_trans_s"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43532">
        <property type="entry name" value="PTHR43532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01207">
        <property type="entry name" value="rmlA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="290" mass="32420" checksum="8AF91277905C8803" modified="2009-02-10" version="1">MKGIILSGGKATRLYPATISVSKQLLPVYDKPMIYYPLSILLFAGIREILIITNPEFLPLYQKLLDDGSQLGIKITYKEQLKPRGIADAFLVGEEFIGDSNVALILGDNLFFGQSFGQAVQKAAQFKEGAVVFAYPVRNPKDFGVVEFDKNGKAISLEEKPEKPKSNWAVPGLYFYDNKVVEIAKNLKPSARGELEITDVNKEYLKKGELKVIPLGRGVAWLDTGTPEGLLEASNFVATIQKRQGFYIACIEEVAYRMGYITREQLYELGKKQEKTDYGKYILELAGEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGW9</accession>
    <name>B7IGW9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polysaccharide export protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_873</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579848.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_873"/>
    <dbReference type="EnsemblBacteria" id="ACJ75333">
        <property type="protein sequence ID" value="ACJ75333"/>
        <property type="gene ID" value="THA_873"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_873"/>
    <dbReference type="eggNOG" id="COG1596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008711_0_0_0"/>
    <dbReference type="OMA" id="VETNYVY"/>
    <dbReference type="OrthoDB" id="687725at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015159">
        <property type="term" value="F:polysaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003715">
        <property type="entry name" value="Poly_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02563">
        <property type="entry name" value="Poly_export"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SLBB" evidence="2">
        <location>
            <begin position="736"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="844" mass="94252" checksum="0783B4666317F55F" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH52</accession>
    <name>B7IH52_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Purine nucleoside phosphorylase</fullName>
            <ecNumber evidence="3">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Inosine-guanosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_957</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_957"/>
    <dbReference type="EnsemblBacteria" id="ACJ75416">
        <property type="protein sequence ID" value="ACJ75416"/>
        <property type="gene ID" value="THA_957"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_957"/>
    <dbReference type="eggNOG" id="COG0005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054456_1_0_0"/>
    <dbReference type="OMA" id="EGVYAQF"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09009">
        <property type="entry name" value="PNP-EcPNPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011270">
        <property type="entry name" value="Pur_Nuc_Pase_Ino/Guo-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011268">
        <property type="entry name" value="Purine_phosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904">
        <property type="entry name" value="PTHR11904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000477">
        <property type="entry name" value="PurNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01700">
        <property type="entry name" value="PNPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01697">
        <property type="entry name" value="PNPH-PUNA-XAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="4">
        <location>
            <begin position="47"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="296" mass="32914" checksum="0F2474BEEB4208D2" modified="2009-02-10" version="1">MFLICHDIIFLDVSKKKAKEELKMKEYIERVNKAVEFLKEHIDNIPKIAIILGSGLHGIAESIENPKKFSYKEIPGFPVSTAPGHKGELIFGKLNGKDVMLMNGRFHYYEGYDMKTVTFPIRVMQLLGVEILIVTNAAGGMNPEFEIGRPMFITDHINMMGDNPLIGPNVEEWGPRFPDMSNAYDKELRQKATEIAKKLNIPFYEGVYVAVSGPNFETPAELKMLRWMGADAVGMSTVPEVIVANHGGLKVLGVSAITDKAVHEDLKPLTAEEVLEVANKTGGMIVKIISELVKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH65</accession>
    <name>B7IH65_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cationic outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Skp family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100935.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_970"/>
    <dbReference type="EnsemblBacteria" id="ACJ75429">
        <property type="protein sequence ID" value="ACJ75429"/>
        <property type="gene ID" value="THA_970"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_970"/>
    <dbReference type="eggNOG" id="COG2825">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136738_0_0_0"/>
    <dbReference type="OMA" id="YYQGKIN"/>
    <dbReference type="OrthoDB" id="1857978at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.910.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005632">
        <property type="entry name" value="Chaperone_Skp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024930">
        <property type="entry name" value="Skp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35089">
        <property type="entry name" value="PTHR35089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03938">
        <property type="entry name" value="OmpH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00935">
        <property type="entry name" value="OmpH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111384">
        <property type="entry name" value="SSF111384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="162" mass="18581" checksum="7C3B098FEC0F70A3" modified="2009-02-10" version="1">MKKLGMVVLASLILLSFVLLFAGGDSTQGPKLAYIDSTKVLQSYDKWIDLQTKYQEDVQFYQKKLNELAAEINDMKEKGAAADLINAKIAEYTQKQKQYSDLLNSEYQKKFAELEQEILQKIAEYAEIMGYDFVFNSKTMAYGNSKYDITAQFIEYLKSMAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH71</accession>
    <name>B7IH71_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_976</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579909.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_976"/>
    <dbReference type="EnsemblBacteria" id="ACJ75435">
        <property type="protein sequence ID" value="ACJ75435"/>
        <property type="gene ID" value="THA_976"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_976"/>
    <dbReference type="eggNOG" id="ENOG5033JAW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1844169_0_0_0"/>
    <dbReference type="OMA" id="ISIECFK"/>
    <dbReference type="OrthoDB" id="1790540at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="143" mass="16283" checksum="0FC78D12BB22F7A4" modified="2009-02-10" version="1">MKKSNLFKFLSYSFSIVSTFPVVTPVLLTIIVLISKGKFLYDFMMPAELILFTIIASLGIITLQIIDKKAFFEYKKLVLLFSLSISNFLAANIYAYLTGLAHGDTNLNGIHLFFITIFVILWHLFAILISIECFKLTKKISTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH76</accession>
    <name>B7IH76_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Redox-active disulfide protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_981</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579912.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_981"/>
    <dbReference type="EnsemblBacteria" id="ACJ75440">
        <property type="protein sequence ID" value="ACJ75440"/>
        <property type="gene ID" value="THA_981"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_981"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_18_1_0"/>
    <dbReference type="OMA" id="KYGVMST"/>
    <dbReference type="OrthoDB" id="2045232at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005243">
        <property type="entry name" value="Redox_disulphide_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36450">
        <property type="entry name" value="PTHR36450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037031">
        <property type="entry name" value="Redox_disulphide_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00412">
        <property type="entry name" value="redox_disulf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin-like_fold" evidence="3">
        <location>
            <begin position="4"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="2">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR037031-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR037031-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="78" mass="8461" checksum="17B151DA1441F4C4" modified="2009-02-10" version="1">MAKKIEILGSGCPRCKQTEKIMKMAVEELNIDATIEKVQDINEIISRGVVATPAVAIDGKIVISGKIPTLEEAKKLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH79</accession>
    <name>B7IH79_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase domain family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_984</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_984"/>
    <dbReference type="EnsemblBacteria" id="ACJ75443">
        <property type="protein sequence ID" value="ACJ75443"/>
        <property type="gene ID" value="THA_984"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_984"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1712397_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="10"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="153" mass="17819" checksum="933F60D2DD87EE0D" modified="2009-02-10" version="1">MFGNQMLLKLGSAVNVPIEDNYCDLVLSLGVIEHYRLKEDLEKSVKEIYRILKPGGIAVIMVPNRISFGVIDRIIQQIFGKWDYGYQKELTPKQLEKIIKKSGFSVIKYKITNLIVVPGVRKNFRFRVIRTFDTFLKKIVNDCGFYLYTYSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH89</accession>
    <name>B7IH89_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CinA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_994</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CinA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_994"/>
    <dbReference type="EnsemblBacteria" id="ACJ75453">
        <property type="protein sequence ID" value="ACJ75453"/>
        <property type="gene ID" value="THA_994"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_994"/>
    <dbReference type="eggNOG" id="COG1058">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_9_3_0"/>
    <dbReference type="OMA" id="PGVPYEM"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00885">
        <property type="entry name" value="cinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00226_B">
        <property type="entry name" value="CinA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036653">
        <property type="entry name" value="CinA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008136">
        <property type="entry name" value="CinA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008135">
        <property type="entry name" value="Competence-induced_CinA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02464">
        <property type="entry name" value="CinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006728">
        <property type="entry name" value="CinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142433">
        <property type="entry name" value="SSF142433"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00200">
        <property type="entry name" value="cinA_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00199">
        <property type="entry name" value="PncC_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="2">
        <location>
            <begin position="15"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="413" mass="46141" checksum="1DBA68886C945B1D" modified="2009-02-10" version="1">METFQKRGETMKTASIIAIGNELVEGIIVDTNSKYISKKLLEYGYKTKIIKTLPDDLDLLVKEIKDSLETTDLVVTTGGLGPTEDDLTREAVSKALEKNLILDENLSKKIIEKAKKFHSYVPKIVERQAMVIEGATVLENPVGTAPGMLLKTPKSTILILPGPPVEMIPIFEKALNLIEKENKIYQRRIKTINIPEAVLVEKYKDIIYKYKEVNVATMASHTSGVELRFTGEKALVDEIVNMLSEKLKDYIYAFDDKTIEEIVFEKLLSKNKTVSFAESCTGGLVSANFVNLSGVSKVFKGSIVAYSNEVKQKLLNVNPKTLEKFGAVSKECVEEMAKGVSKLLDTDYSVAISGIAGPTGGTKEKPVGTVWICAYSRENDIFMTERFYFKGNRQTIRNRSTLHAFDMLRRILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHA5</accession>
    <name>B7IHA5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane associated methyl-accepting chemotaxis protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1011"/>
    <dbReference type="EnsemblBacteria" id="ACJ75469">
        <property type="protein sequence ID" value="ACJ75469"/>
        <property type="gene ID" value="THA_1011"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1011"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_813615_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038158">
        <property type="entry name" value="H-NOX_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011644">
        <property type="entry name" value="Heme_NO-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07700">
        <property type="entry name" value="HNOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="HNOB" evidence="2">
        <location>
            <begin position="2"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="341" mass="38980" checksum="2C6461712BD83182" modified="2009-02-10" version="1">MKSFVMNIWITTWKKLYGENIVNELSSKFNLDEQNLLIPTNNVPDELVVNFSRELAKKVGKTYEELWQETGYHNIKSFHSFYPSYFKKEGVLSFLSAMDSVHRALTRRIKGAKPPRIIYNYIDEKTAVIRYESHRDFRNYFLGLLKGAADFFNDPLKIEILKQDMNSNGSFIEVKVTATKPYGKSVKLKLYKAISFGLLKSFSTNLTVIIPILTFVLSFLFTKYLGALPGSILTSISILIGLLLINKDLKNATKSVEKIINIYKEKDFNDILMISGEKDFEKLSKSNIKALSNLREFLIGFQGDTEEILNFSKKTLESSDAIQEEIGTMKELYCSPEGLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHB8</accession>
    <name>B7IHB8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein YddE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579940.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1024"/>
    <dbReference type="EnsemblBacteria" id="ACJ75482">
        <property type="protein sequence ID" value="ACJ75482"/>
        <property type="gene ID" value="THA_1024"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1024"/>
    <dbReference type="eggNOG" id="COG0384">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048756_0_2_0"/>
    <dbReference type="OMA" id="CHAWLQA"/>
    <dbReference type="OrthoDB" id="1468060at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003719">
        <property type="entry name" value="Phenazine_PhzF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13774">
        <property type="entry name" value="PTHR13774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02567">
        <property type="entry name" value="PhzC-PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016184">
        <property type="entry name" value="PhzC_PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00654">
        <property type="entry name" value="PhzF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="1">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR016184-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="279" mass="31760" checksum="6D8BA16F9CA1B74B" modified="2009-02-10" version="1">MNFYVVDAFANDPFKGNPAGVVILKEDIPKSIKQLIASEVKFSETAFIKKISENYFKIEYFTPVSEIDLCGHATIATFYVLKEKNLVFPNNKYNLHTNVGKLTILIENDLVLMEQAKPELGKVYDKETIAEILGINSEELDERFKLQASYTGLWDLLIPIKKKETLLKLEPNLEKIKEFTKENNIVSFHLFTLDEKEALANTRDFAPLYGINEEAATGTANGALLYYLYKNNLIDEKKVYKIIQGESMNRKSEIFGKIENEKVFIGGKAKIIIEGNLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHC6</accession>
    <name>B7IHC6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579945.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1032"/>
    <dbReference type="EnsemblBacteria" id="ACJ75490">
        <property type="protein sequence ID" value="ACJ75490"/>
        <property type="gene ID" value="THA_1032"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1032"/>
    <dbReference type="eggNOG" id="COG3629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1106693_0_0_0"/>
    <dbReference type="OMA" id="FWGENAR"/>
    <dbReference type="OrthoDB" id="1990552at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="251" mass="30037" checksum="B3780118FB963FB1" modified="2009-02-10" version="1">MIYFKMFGDFKVFVDGKEVETKNNKNLKLLFYIISSRKSYIPKEEIYDVFWNNFTRDYARKNLNVQMYNLKKTYLFLEDVIKNYRENIYIDRDKISSDYEFFMDNIGSNSLKALKVYSGDFLEGYDDDWIVEKRKQCRKLANLALYFYNEEAKKVEILLEIQKDMREKPYVILFLNEIKNLRMRKGDFLLNLENGSLVLLEKGNKTVSQAVDGFLKRSGIERAKMLNEDEALNLINFKNSDKEEKIYEENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHD8</accession>
    <name>B7IHD8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Xylose isomerase domain protein TIM barrel, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579953.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1044"/>
    <dbReference type="EnsemblBacteria" id="ACJ75502">
        <property type="protein sequence ID" value="ACJ75502"/>
        <property type="gene ID" value="THA_1044"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1044"/>
    <dbReference type="eggNOG" id="ENOG5030FUU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1336989_0_0_0"/>
    <dbReference type="OMA" id="NIVEIHM"/>
    <dbReference type="OrthoDB" id="1007505at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="203" mass="23851" checksum="CC02BCCB248970FB" modified="2009-02-10" version="1">MKIGATSWLIPGGYLENVKLVAEIVDFVELLVFSFDMETRHMLKNELPELYKISKKYGLEYTVHLPTDCINNVEDAFNFFEESNLPILNYVVHPIDCIEPLLKKSDKISVENLKKDILIHNRTVFDIGHHILGMKVTKEFFENTIEFHLMGVKGDQDHLKLDKETLLLSKEYMELMPNLKYICFEVFDINDFLDSYNLWRENL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHE6</accession>
    <name>GLYA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="1">SHMT</shortName>
            <shortName evidence="1">Serine methylase</shortName>
            <ecNumber evidence="1">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glyA</name>
        <name type="ordered locus">THA_1054</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579961.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHE6"/>
    <dbReference type="SMR" id="B7IHE6"/>
    <dbReference type="STRING" id="484019.THA_1054"/>
    <dbReference type="EnsemblBacteria" id="ACJ75510">
        <property type="protein sequence ID" value="ACJ75510"/>
        <property type="gene ID" value="THA_1054"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1054"/>
    <dbReference type="eggNOG" id="COG0112">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_2_1_0"/>
    <dbReference type="OMA" id="SHPAGLI"/>
    <dbReference type="OrthoDB" id="861782at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="PTHR11680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000116836" description="Serine hydroxymethyltransferase">
        <location>
            <begin position="1"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="351"/>
            <end position="353"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in substrate specificity" evidence="1">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <sequence length="424" mass="46865" checksum="CBB02F87A5EC29C0" modified="2009-02-10" version="1">MWENVKKTDPEIYDVILKEWERQEYGLELIASENFASLAVIEAMGSVLTNKYAEGYPGRRYYGGCEWVDVAEKLARDRAKELFNVKYANVQPHSGSQANMGAYFAVSEPGDTIMGMSLSHGGHLTHGASVNFSGRIYNVVPYGVNPETEVIDYDEVRDLALKHKPKIIVAGGSAYSRIIDFKKFREIADEVGAYLIVDMAHFAGLVAAGIYPNPAEYAHIVTSTTHKTLRGPRGGMILTNDNELYKAINKSIFPGIQGGPLMHVIAAKAVCFKEALTDEFKEYQKQVVKNAKTLAAELEKRGLRIVSGGTDTHLMLVDLNPLNVTGKAAEIALGKCHITVNKNTIPNETRSPFIASGIRLGTPALTTRGMKESEMEEIAELIVDVLKHVKDEEGNVDEEIVEKTQKKVKDLCTRFPLYEGKIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHF7</accession>
    <name>B7IHF7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase</fullName>
            <shortName evidence="10">NN:DBI PRT</shortName>
            <ecNumber evidence="4 10">2.4.2.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">N(1)-alpha-phosphoribosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cobT2</name>
        <name evidence="10" type="synonym">cobT</name>
        <name evidence="11" type="ordered locus">THA_1065</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>5,6-dimethylbenzimidazole + nicotinate beta-D-ribonucleotide = alpha-ribazole 5'-phosphate + H(+) + nicotinate</text>
            <dbReference type="Rhea" id="RHEA:11196"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15890"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="EC" id="2.4.2.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-ribazole from 5,6-dimethylbenzimidazole: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the CobT family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.21" evidence="4 10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579972.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1065"/>
    <dbReference type="EnsemblBacteria" id="ACJ75521">
        <property type="protein sequence ID" value="ACJ75521"/>
        <property type="gene ID" value="THA_1065"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1065"/>
    <dbReference type="eggNOG" id="COG2038">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002982_0_0_0"/>
    <dbReference type="OMA" id="AWMRKCA"/>
    <dbReference type="OrthoDB" id="1765140at2"/>
    <dbReference type="UniPathway" id="UPA00061">
        <property type="reaction ID" value="UER00516"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02439">
        <property type="entry name" value="DMB-PRT_CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1610.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00230">
        <property type="entry name" value="CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003200">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017846">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023195">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02277">
        <property type="entry name" value="DBI_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="SSF52733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03160">
        <property type="entry name" value="cobT_DBIPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="7 10 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 11" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ75521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="344" mass="37671" checksum="B87BBB8F1A1FD9DC" modified="2009-02-10" version="1">MDLKQKVIDRLNNLTKPVRSLGYLEDIALKMALIQNKEIPELFNDKRVYVFASDHGVVENGVSAYPKDVTYQMVFNFLNGGAAINVFSRHVGAKVYVVDAGVDYDFQDNHSLIKMKVGYGTKDFTKGPAMSREEAEKCIELGKKIAREAIKEGADLLAVGDMGIGNTTTASAIAVAFGYNIDDIIDIGTPLDSEGIKRKREAVLRAIEVNRPDPNDPIDVLSKVGGYCIGEMAGFILEAAHSKVPVVIDGFPTTSAFLISYYLDKDVINYVFSGHKSYVKGHKVILDSLNLRPILDLDMRLGEGTGAVLSMPLIEAAIKMIREMATFDSANVSKGIDQNLEDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHG7</accession>
    <name>B7IHG7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Precorrin-6x reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cobK</name>
        <name evidence="4" type="ordered locus">THA_1075</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579981.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1075"/>
    <dbReference type="EnsemblBacteria" id="ACJ75531">
        <property type="protein sequence ID" value="ACJ75531"/>
        <property type="gene ID" value="THA_1075"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1075"/>
    <dbReference type="eggNOG" id="COG2099">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068627_0_0_0"/>
    <dbReference type="OMA" id="THPFAIN"/>
    <dbReference type="OrthoDB" id="1726104at2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016994">
        <property type="term" value="F:precorrin-6A reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003723">
        <property type="entry name" value="Precorrin-6x_reduct"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36925">
        <property type="entry name" value="PTHR36925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02571">
        <property type="entry name" value="CbiJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00715">
        <property type="entry name" value="precor6x_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51014">
        <property type="entry name" value="COBK_CBIJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="245" mass="27890" checksum="5DC80B9976A21155" modified="2009-02-10" version="1">MILVLSGTSVGRKVADALSRKYKVLVSTKTEYGKKLINENKNLKAVYGELDRESLKSLILKNNIKVIVDATHPFATIISKIAFSVSKELNVDYIRYLEEEEKVSYENAVYVENHNQAKEKCHDFENIFLTIGTKDLAVYKDLIDSKNVFVRVLPTLDSIKICEDLNILPKNIIAMQGPFSKELNYILFKETEAQVVVTKESNLFEKVEAAKRLGIHLIIVKRPKEVGRKKVSSIDELMRVIKDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHH2</accession>
    <name>B7IHH2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="3">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin</text>
            <dbReference type="Rhea" id="RHEA:15613"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:58827"/>
            <dbReference type="EC" id="1.3.1.76"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579986.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1080"/>
    <dbReference type="EnsemblBacteria" id="ACJ75536">
        <property type="protein sequence ID" value="ACJ75536"/>
        <property type="gene ID" value="THA_1080"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1080"/>
    <dbReference type="eggNOG" id="COG1648">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_8_3_0"/>
    <dbReference type="OMA" id="VNMMVKQ"/>
    <dbReference type="OrthoDB" id="1185397at2"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006367">
        <property type="entry name" value="Sirohaem_synthase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="PTHR35330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01470">
        <property type="entry name" value="cysG_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="148" mass="16707" checksum="5EA5D96C23BE6BE5" modified="2009-02-10" version="1">MDYLPVILNIKGKKCLIVGGGKVAARKIKYLLGRVEITVISKDFCREIKDMNGIKLVRKAYDSSDLDGFDIVIAATNDERTNKQIFLDAQNKNVLVNNATSKEYCDFLMPAFFNYKDFIVAVSSFGNSPKRAKNLKEKIKKYLEGLDV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHH3</accession>
    <name>GSA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <shortName evidence="1">GSA</shortName>
            <ecNumber evidence="1">5.4.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamate-1-semialdehyde aminotransferase</fullName>
            <shortName evidence="1">GSA-AT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemL</name>
        <name type="ordered locus">THA_1081</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579987.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHH3"/>
    <dbReference type="SMR" id="B7IHH3"/>
    <dbReference type="STRING" id="484019.THA_1081"/>
    <dbReference type="EnsemblBacteria" id="ACJ75537">
        <property type="protein sequence ID" value="ACJ75537"/>
        <property type="gene ID" value="THA_1081"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1081"/>
    <dbReference type="eggNOG" id="COG0001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_1_5_0"/>
    <dbReference type="OMA" id="AQTWRFP"/>
    <dbReference type="OrthoDB" id="493793at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00713">
        <property type="entry name" value="hemL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000382387" description="Glutamate-1-semialdehyde 2,1-aminomutase">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00375"/>
        </source>
    </evidence>
    <sequence length="423" mass="46921" checksum="2E1E249686DE3C17" modified="2009-02-10" version="1">MFEIAKKYMPGGVNSPVRAFKSVEMEPIFVKSAKKSKLIDINNKEYIDYIQSWGALILGHAHEEVVEEIKKQAELGTSYGLCHELEVEMARLIVKHIPSVEMVRMVNSGTEAVMSAIRLARAYTNRELIVKFEGCYHGHSDGLLVKAGSGALTFGTPSSKGVTEKIVSNTLVARYNDIESVKELFDKYGENIACVIVEPVAGNMGVVLPKQGFLEGLREITKEYGSLLIFDEVITGFRVSINGAQGYYNVFPDITTLGKIIGGGLPVGAYGGRKEIMELISPQGPVYQAGTLSGNPLTLAAGIKTIKIIENDPDFYSKLDELGKYFEEGIVSALGDFDVKINRIKSMLSFFFSKQEVDSYEKVINSDVEIYKKLFKCLFENGILLPPSPFESLFISSSHTKEDIEKTVYYFEKFSKKLKEGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHH7</accession>
    <name>B7IHH7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cobalt ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1085</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579990.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1085"/>
    <dbReference type="EnsemblBacteria" id="ACJ75541">
        <property type="protein sequence ID" value="ACJ75541"/>
        <property type="gene ID" value="THA_1085"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1085"/>
    <dbReference type="eggNOG" id="COG1122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_0"/>
    <dbReference type="OMA" id="ANDQLFM"/>
    <dbReference type="OrthoDB" id="1752365at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="242" mass="27779" checksum="DA03D32D37A5F7BD" modified="2009-02-10" version="1">MLRLENVYFSYESKNYILEDISLEFKKGLKIAILGRNGCGKTTLMLLCSGILKPKKGKIFIDDLEVKNPRLLKKHVGIVFQNPDTQLLAAKVYQDISFGLFNLGFGKDIVKKKVEEIIKRLGIEHLKNKPTQFLSYGQKRLVSIADILVMEPEYIFLDEPEAYLDYKSFLKVQEIISELPKEGKSVIISTHDSDFAFEWADYIYVIDNKTVALHGTPDYVFSQLEDLQKIGLKIPLIKRLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHK2</accession>
    <name>B7IHK2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucose-1-phosphate thymidylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580006.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1113"/>
    <dbReference type="EnsemblBacteria" id="ACJ75566">
        <property type="protein sequence ID" value="ACJ75566"/>
        <property type="gene ID" value="THA_1113"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1113"/>
    <dbReference type="eggNOG" id="COG0663">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_0"/>
    <dbReference type="OMA" id="RRNWIDA"/>
    <dbReference type="OrthoDB" id="1004719at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04189">
        <property type="entry name" value="G1P_TT_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005908">
        <property type="entry name" value="G1P_thy_trans_l"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01208">
        <property type="entry name" value="rmlA_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="359" mass="40050" checksum="F7EE025972DB5BAC" modified="2009-02-10" version="1">MKALILCAGKGTRLRPLTFTIAKHLIPIANKPVISYSLEKIKSVGIEEVGIVVNPENIKDFKNFFGNGEKFGLKIEYILQQEPKGLAHAVMVSKDFLKDDDFLMYLGDNLILDDITSFVEEFKNDEDMKASILLSPVKDPSRFGVAVVKGGKIIEVVEKPKEPISNLAIIGLYLFRNTIFEGIENIKPSWRGELEITDAIGYLIKNNYKVKGHVVYGWWKDTGKPEDLIEANRKILDDNHFKVQIDGIVDTSSVIQGRVSIGENSEIINSTIRGPVVIGKNCIIKDSYIGPYTSIGNNAVIEDCEIENSILMSHVKLMNLPYPIDSSLIGKNVQIVNNEKKPKAMKFVIGDMGKVEIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHM3</accession>
    <name>B7IHM3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">W family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cysT</name>
        <name evidence="6" type="ordered locus">THA_1134</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101245.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1134"/>
    <dbReference type="EnsemblBacteria" id="ACJ75587">
        <property type="protein sequence ID" value="ACJ75587"/>
        <property type="gene ID" value="THA_1134"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1134"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_4_1_0"/>
    <dbReference type="OMA" id="LRWHPGY"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="50"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="243" mass="27548" checksum="97626E7A6301D91A" modified="2009-02-10" version="1">MKFLYGLILFILIWWIFSIIIDSQLILPTPISVGRVFIDLVQTKTLWSSLLSTVLKGIIALAFTIILGFFFGFLMGIFDVLYELFRPAFTIFQSVPVISWLVLVIFVWGIGFKGPIVITVLSLLPNTTFAIAQGVKNTDKKLIEMAYVYKVPKKRIVKDIYIGSVIPFLFTALEVISGNIWKVIIVSEYLAGNEGIGVQIAWARQYVNVPKVYALTIFAILLGISSERLIKFIIKRKEFYYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHP0</accession>
    <name>B7IHP0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1152"/>
    <dbReference type="EnsemblBacteria" id="ACJ75604">
        <property type="protein sequence ID" value="ACJ75604"/>
        <property type="gene ID" value="THA_1152"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1152"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_344495_0_0_0"/>
    <dbReference type="OMA" id="MKGPYVK"/>
    <dbReference type="OrthoDB" id="209126at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011045">
        <property type="entry name" value="N2O_reductase_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50974">
        <property type="entry name" value="SSF50974"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="812" mass="93007" checksum="1B5B5B8CB65026AA" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHQ4</accession>
    <name>RUVA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Holliday junction ATP-dependent DNA helicase RuvA</fullName>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ruvA</name>
        <name type="ordered locus">THA_1166</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex with RuvB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101324.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHQ4"/>
    <dbReference type="SMR" id="B7IHQ4"/>
    <dbReference type="STRING" id="484019.THA_1166"/>
    <dbReference type="EnsemblBacteria" id="ACJ75618">
        <property type="protein sequence ID" value="ACJ75618"/>
        <property type="gene ID" value="THA_1166"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1166"/>
    <dbReference type="eggNOG" id="COG0632">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087936_3_1_0"/>
    <dbReference type="OMA" id="VGMAVQC"/>
    <dbReference type="OrthoDB" id="1370858at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009379">
        <property type="term" value="C:Holliday junction helicase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14332">
        <property type="entry name" value="UBA_RuvA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00031">
        <property type="entry name" value="DNA_helic_RuvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013849">
        <property type="entry name" value="DNA_helicase_Holl-junc_RuvA_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000085">
        <property type="entry name" value="RuvA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011114">
        <property type="entry name" value="RuvA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036267">
        <property type="entry name" value="RuvA_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07499">
        <property type="entry name" value="RuvA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01330">
        <property type="entry name" value="RuvA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46929">
        <property type="entry name" value="SSF46929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00084">
        <property type="entry name" value="ruvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_1000195179" description="Holliday junction ATP-dependent DNA helicase RuvA">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00031"/>
        </source>
    </evidence>
    <sequence length="189" mass="20957" checksum="D72F82EC92380252" modified="2009-02-10" version="1">MIHALNGKVESIENGKVYVNVNDLIYEIIVGDTGDFEEYINRNVKIYTKMVVNDDGISLYGFLEVIKLKLFEKLIGVNKLGPKSALKIISSNSVESVVSAIINEDVKALSSLPGIGPKTAERIVLELKDTIKELDVSINEKDRKVLEAIEALVTLGFNRNQAKKAVNKVAAKDDKLDDIIKKALRFLSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHT4</accession>
    <name>RL20_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplT</name>
        <name type="ordered locus">THA_1198</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101417.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHT4"/>
    <dbReference type="SMR" id="B7IHT4"/>
    <dbReference type="STRING" id="484019.THA_1198"/>
    <dbReference type="EnsemblBacteria" id="ACJ75648">
        <property type="protein sequence ID" value="ACJ75648"/>
        <property type="gene ID" value="THA_1198"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1198"/>
    <dbReference type="eggNOG" id="COG0292">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123265_0_1_0"/>
    <dbReference type="OMA" id="GRRKNVW"/>
    <dbReference type="OrthoDB" id="1932144at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07026">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00382">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005813">
        <property type="entry name" value="Ribosomal_L20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035566">
        <property type="entry name" value="Ribosomal_protein_L20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10986">
        <property type="entry name" value="PTHR10986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00453">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00062">
        <property type="entry name" value="RIBOSOMALL20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74731">
        <property type="entry name" value="SSF74731"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01032">
        <property type="entry name" value="rplT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00937">
        <property type="entry name" value="RIBOSOMAL_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000122383" description="50S ribosomal protein L20">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00382"/>
        </source>
    </evidence>
    <sequence length="118" mass="13644" checksum="3241950700DF5870" modified="2009-02-10" version="1">MRVKNAVNAKKKRRKILKAVKGYHGALSRRYRLAKQAYIKAKKHAYVGRKLKKRDFRKLWITRINIAARNEGLKYNELIHGLKLAGVAINRKMLSELAVNDPESFKEYVNIAKQAIGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHU4</accession>
    <name>EFTU_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor Tu</fullName>
            <shortName evidence="2">EF-Tu</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tuf</name>
        <name type="ordered locus">THA_1213</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101434.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHU4"/>
    <dbReference type="SMR" id="B7IHU4"/>
    <dbReference type="STRING" id="484019.THA_1213"/>
    <dbReference type="PRIDE" id="B7IHU4"/>
    <dbReference type="EnsemblBacteria" id="ACJ75658">
        <property type="protein sequence ID" value="ACJ75658"/>
        <property type="gene ID" value="THA_1213"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1213"/>
    <dbReference type="eggNOG" id="COG0050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_0_1_0"/>
    <dbReference type="OMA" id="EGDKEWG"/>
    <dbReference type="OrthoDB" id="621774at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000201419" description="Elongation factor Tu">
        <location>
            <begin position="1"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="10"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="60"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="81"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="174"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <sequence length="400" mass="44394" checksum="DEDDE44CFD19AF81" modified="2009-02-10" version="1">MAKEKFVRSKPHLNVGTIGHIDHGKTTLTAAITKYLSFFGRADYTPYEQIDKAPEEKERGITINISHIEYETETRHYAHIDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVNVPAMIVFINKTDMVDDEELIDLVEMEVRELLNKYEFPGDDLPVVRGSALKAVEGPDDPNDPVYAPIKELLDTMDSYFPEPQRETDKPFLMPVEDVFSITGRGTVVTGRIERGVIRPGDEVEIIGMSYDIKKTVVTSVEMFRKILDEGLAGDNVGCLLRGIDKDEVERGQVLAKPGSITPHTTFKAQVYVLKKEEGGRHTPFQKGYKPQFFIRTADVTGELIDFPAGVEMVMPGDNVEMTIKLIYPVAIEEGMRFAIREGGRTVGAGVVTAIVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHW0</accession>
    <name>RS8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsH</name>
        <name type="ordered locus">THA_1229</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S5 and S12.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101465.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHW0"/>
    <dbReference type="SMR" id="B7IHW0"/>
    <dbReference type="STRING" id="484019.THA_1229"/>
    <dbReference type="EnsemblBacteria" id="ACJ75674">
        <property type="protein sequence ID" value="ACJ75674"/>
        <property type="gene ID" value="THA_1229"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1229"/>
    <dbReference type="eggNOG" id="COG0096">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098428_0_2_0"/>
    <dbReference type="OMA" id="FDQGYIL"/>
    <dbReference type="OrthoDB" id="1692188at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_B">
        <property type="entry name" value="Ribosomal_S8_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_S8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_S8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="PTHR11758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="SSF56047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000140625" description="30S ribosomal protein S8">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <sequence length="134" mass="15171" checksum="FFF0F4C31B9ED490" modified="2009-02-10" version="1">MWSDPIADMLTRIRNANVALKEQVDVPASNLKKEIAEILKREGFIKDYTYIEDGKQGIIRIHMKYKGTRRNRERVIHGIVRVSKPGRRIYVGKDNLPKVKNGLGIAILTTSKGVLTDKQAREIGVGGEVIAYIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICP1</accession>
    <name>B7ICP1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1323</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1323"/>
    <dbReference type="EnsemblBacteria" id="ACJ75768">
        <property type="protein sequence ID" value="ACJ75768"/>
        <property type="gene ID" value="THA_1323"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1323"/>
    <dbReference type="eggNOG" id="COG4586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_0"/>
    <dbReference type="OMA" id="DIRTHEF"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="18"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="290" mass="33484" checksum="EEED231FBE40CFC9" modified="2009-02-10" version="1">MENVSFSISKGEKVAVLGLNGSGKSTLFKILTGIIPPSSWVCKCMGYDPFSDRKRYVKNIGVLLGQKSLLIPELTVYDSLKLYKAVYQLNEKQLDERLKVFDEYFGIEKLLSRIVRTLSLGERMKAEIFMASIHDLSIFFFDEPTIGLDVNAKSAFKEFLLNYPFGSEKTVFIITHEFYVIKDFCTRILLLDNGKLKLDIETKHLKNKFGYKNLVIDFEGKPTKTTVENIRLFGYPIEIEKNSVKIKVPYENKEEIEEIRGILSKSLKRNFELLKIGVELSLRRLSYFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICQ2</accession>
    <name>B7ICQ2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lipopolysaccharide biosynthesis protein BplA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1334</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580153.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1334"/>
    <dbReference type="EnsemblBacteria" id="ACJ75779">
        <property type="protein sequence ID" value="ACJ75779"/>
        <property type="gene ID" value="THA_1334"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1334"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_1_0_0"/>
    <dbReference type="OMA" id="VPDMTRW"/>
    <dbReference type="OrthoDB" id="1465613at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="2">
        <location>
            <begin position="140"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="360" mass="40810" checksum="51A28F09AB18783A" modified="2009-02-10" version="1">MLKIGLIGCGRIATKKHTEALIQNKELFDVVAVCDIVKEKASNCAEIFEERLGKRPEVETDFRKLLKREDIDAVAIATDSGSHYEIAVEALNNGKHVLVEKPMALSTKHADEMIKLSKEKNLKLEVCFQNRFNPPIQELRKKIEGKDFGKVHYGVATIRWNRNEGYYKQASWRGTWEKDGGALMNQCTHNIDLLQWMLGGEIEEVYGVIKNFNHPYIEAEDFGGAIIKFKDGKIGMIEGTTTIYPKNLHEKLAIFGEKGTVVIGGLAVNRIEEWRFEGEEGHPFMSLPDPDTVYGSGHVPLYRDFYESIVNDRKPYISGEEGKKAVEIVLAIYKSALEGKPVKFPFEFSTYEIKGKNYEV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICR4</accession>
    <name>RL7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L7/L12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplL</name>
        <name type="ordered locus">THA_1346</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Part of the ribosomal stalk of the 50S ribosomal subunit. Forms a multimeric L10(L12)X complex, where L10 forms an elongated spine to which 2 to 4 L12 dimers bind in a sequential fashion. Binds GTP-bound translation factors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101702.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ICR4"/>
    <dbReference type="SMR" id="B7ICR4"/>
    <dbReference type="STRING" id="484019.THA_1346"/>
    <dbReference type="EnsemblBacteria" id="ACJ75791">
        <property type="protein sequence ID" value="ACJ75791"/>
        <property type="gene ID" value="THA_1346"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1346"/>
    <dbReference type="eggNOG" id="COG0222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086499_3_2_0"/>
    <dbReference type="OMA" id="LEDKWGV"/>
    <dbReference type="OrthoDB" id="1822695at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00387">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00368">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_L7/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_L7/12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_L7/L12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008932">
        <property type="entry name" value="Ribosomal_L7/L12_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036235">
        <property type="entry name" value="Ribosomal_L7/L12_oligo_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="PTHR45987"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16320">
        <property type="entry name" value="Ribosomal_L12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48300">
        <property type="entry name" value="SSF48300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="SSF54736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00855">
        <property type="entry name" value="L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000121498" description="50S ribosomal protein L7/L12">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00368"/>
        </source>
    </evidence>
    <sequence length="128" mass="13369" checksum="D2964482992BF7BD" modified="2009-02-10" version="1">MTLEEIVSAIEQLTVAELAELVKMLEDKFGVSASAPVMAMPMAGAAAGGAAAAEEKTEFDVVLKSFGAKKIEVIKVIREITGLGLKEAKDLVEKAGTPDAVVKQGAAKEEAEEIKKKLEAAGAEVELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICS0</accession>
    <name>B7ICS0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="6">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="6">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">coaD</name>
        <name evidence="8" type="synonym">coaD2</name>
        <name evidence="8" type="ordered locus">THA_1352</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the bacterial CoaD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580169.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1352"/>
    <dbReference type="EnsemblBacteria" id="ACJ75797">
        <property type="protein sequence ID" value="ACJ75797"/>
        <property type="gene ID" value="THA_1352"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1352"/>
    <dbReference type="eggNOG" id="COG0669">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="PPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
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        <property type="entry name" value="PPAT"/>
    </dbReference>
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        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF1">
        <property type="entry name" value="PTHR21342:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01020">
        <property type="entry name" value="LPSBIOSNTHSS"/>
    </dbReference>
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        <property type="entry name" value="coaD_prev_kdtB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4 6" id="KW-0173">Coenzyme A biosynthesis</keyword>
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    <keyword evidence="3 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="7">
        <location>
            <begin position="4"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="8"/>
            <end position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="87"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="122"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="158" mass="18481" checksum="E8500201D869117E" modified="2009-02-10" version="1">MKAIYPGSFDPITFGHLDIIKRASKIFSEVFVVVMENKRKKYTFSLEERIEMIKECTKDIANIKVDYFNGLLIDYLQKNKIDVIIRGLRAVTDFEYELQMAMANKEMCPHTDTVFLMTDKKYSFISSSLVKEVAYFGGDISRWVPKNVEEKLIQKLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICY4</accession>
    <name>B7ICY4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="6" type="ordered locus">THA_1417</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1417"/>
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        <property type="protein sequence ID" value="ACJ75861"/>
        <property type="gene ID" value="THA_1417"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:sodium ion export across plasma membrane"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Na_pump_deCOase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04277">
        <property type="entry name" value="OAD_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="115" mass="13493" checksum="FA4A375122820C73" modified="2009-02-10" version="1">MQMFIITFIGLVTVFLIFFILYFVFLLFGFFSKKASMKLPKKIHSQELTNEKNILLESKESEEEIAAVMAAIYALMGSNIRIISVSKKNRKREWTFWKKTGWRGVKGWTESSRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICY8</accession>
    <name>B7ICY8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1421</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580217.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1421"/>
    <dbReference type="EnsemblBacteria" id="ACJ75865">
        <property type="protein sequence ID" value="ACJ75865"/>
        <property type="gene ID" value="THA_1421"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1421"/>
    <dbReference type="eggNOG" id="ENOG5033VT8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1141233_0_0_0"/>
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    <dbReference type="OrthoDB" id="1496724at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="60"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="239" mass="27354" checksum="B9768D18659E6A26" modified="2009-02-10" version="1">MKIQGIIFTTNEVPRKLYKLKSGKVRELKKSGPVILNPGDYIAIAEYLLEIPLFGDVSAIEESEIEEVELSDEFENILKRLIELRKNKNAEENFSLEDFVIDEENIDNVIEEVETLLSLASDNLPDDEEEAINLIESIDDSKMITKVNYIKSFFKKFPDSKLGGELILDTAKKVYSTLGDRYIAKLLCKKIILHYSDDLNLCLNALNLLALVYKEEGNVMWLTYSEIAKHVEVMLNENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICZ3</accession>
    <name>B7ICZ3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1426</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580220.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1426"/>
    <dbReference type="EnsemblBacteria" id="ACJ75870">
        <property type="protein sequence ID" value="ACJ75870"/>
        <property type="gene ID" value="THA_1426"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1426"/>
    <dbReference type="HOGENOM" id="CLU_2385152_0_0_0"/>
    <dbReference type="OMA" id="TFLIIND"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="94" mass="10497" checksum="002A30D16B8337A3" modified="2009-02-10" version="1">MLVKFVGSIKYLLGKSSIEIDFKGENDLFKQISKKLNKEVLIKIDKENKKTFLIINDTQPIKLSVVILNNGENILRKSKIEDGELAIILPVGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID29</accession>
    <name>B7ID29_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Regulatory protein RecX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">recX</name>
        <name evidence="5" type="ordered locus">THA_1463</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Modulates RecA activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the RecX family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580239.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1463"/>
    <dbReference type="EnsemblBacteria" id="ACJ75906">
        <property type="protein sequence ID" value="ACJ75906"/>
        <property type="gene ID" value="THA_1463"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1463"/>
    <dbReference type="eggNOG" id="COG2137">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066607_5_1_0"/>
    <dbReference type="OMA" id="EPDIINC"/>
    <dbReference type="OrthoDB" id="2025178at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01114">
        <property type="entry name" value="RecX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003783">
        <property type="entry name" value="Regulatory_RecX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33602">
        <property type="entry name" value="PTHR33602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02631">
        <property type="entry name" value="RecX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="130" mass="15478" checksum="FBD9622B5A17BBE7" modified="2009-02-10" version="1">MRKKNPINDALRLLKFRIRSEKEIYFRLKDKGYSDEEIKKTIEELKSKGFLDDEKFAYLYAYDSLTLKKKGPFLIKRELLNLGVDEYIIDDAISKVLSEVDIEQIKRELVKDMDARKAREYLYRRGFGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID63</accession>
    <name>B7ID63_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mate efflux family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102002.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1499"/>
    <dbReference type="EnsemblBacteria" id="ACJ75940">
        <property type="protein sequence ID" value="ACJ75940"/>
        <property type="gene ID" value="THA_1499"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1499"/>
    <dbReference type="eggNOG" id="COG0534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_5_3_0"/>
    <dbReference type="OMA" id="DYKHEMV"/>
    <dbReference type="OrthoDB" id="1415498at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="437" mass="49014" checksum="074B859CFC240FA2" modified="2009-02-10" version="1">MGLPTAIGFSFQMFYDVVDLFWIGKISSEAIAGVGIFSTVFWVVEALNEVIGVSSISLISQSFGKKDYKRTNLAIEQTITFKFLVALLGSFFLYVFLKPILGLFSEENEVISLGIEYGYIRIFFLPIMFSSYSVNTALRSIGDSKTPMNLMILSSILNIILDPIFMFEKVPILGLKGLNLGVSGAAWATIVSQTVAFLIGFYFLFSGIENIKPSIKGLFKLNWEIDKKLITIGLPNGFEVFMRNMSGAIILYFVSRYGTTAVSAYTIGGRIFGFLFMPLMGLLMGGSAIIGQTLGAEKVERAEKVAKVSGWLTATLTFIFVILIVVFAPEMMQLFTKESEIIDIGVLFLRYSVIGLVFLGYGIGISIVFTGSGYNMPYFFMSLFSRWIVQIPLMYIFSYILKLSIIWLWLSFTFGDIAEFLIAIYFYKKGDWKYKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID83</accession>
    <name>B7ID83_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative gluconeogenesis factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1519</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for morphogenesis under gluconeogenic growth conditions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gluconeogenesis factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580263.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1519"/>
    <dbReference type="EnsemblBacteria" id="ACJ75960">
        <property type="protein sequence ID" value="ACJ75960"/>
        <property type="gene ID" value="THA_1519"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1519"/>
    <dbReference type="eggNOG" id="COG0391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044041_0_1_0"/>
    <dbReference type="OMA" id="LCGDDDW"/>
    <dbReference type="OrthoDB" id="665169at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00973">
        <property type="entry name" value="Gluconeogen_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010119">
        <property type="entry name" value="Gluconeogen_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30135">
        <property type="entry name" value="PTHR30135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="SSF142338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01826">
        <property type="entry name" value="CofD_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="312" mass="34810" checksum="4CFB5F4D47E57744" modified="2009-02-10" version="1">MNLTVVGGGTGVSTLLRGLKYFNNIDLKAIIAVTDEGGSSGVLRKEYNVLPPGDVRNNLVALAKDEEILGQLFSYRFKDGFLAGHSVGNIILTALTKILGSFTKAIEYASEVLAINGKVIPISEDLIRLVAEYEDGTKAFGESEIMKITKKRIKKVYLNRDAKINLDAQKALVNSDYIILGPGSIYTSIITNFLVDGFKEAVNYSNAKIIYISNLMTQASESYNFTLFDHVSVIERYLERKVDMIIASRSNIPEYILERYENEGSIPVKVDIENDERLIFEDLLEVKVDSDGKEKIRHNSMKLASLIIKLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDC8</accession>
    <name>B7IDC8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">GatB/Yqey</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1567</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102292.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1567"/>
    <dbReference type="EnsemblBacteria" id="ACJ76005">
        <property type="protein sequence ID" value="ACJ76005"/>
        <property type="gene ID" value="THA_1567"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1567"/>
    <dbReference type="eggNOG" id="COG1610">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079430_2_1_0"/>
    <dbReference type="OMA" id="AMGAVMK"/>
    <dbReference type="OrthoDB" id="1498741at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016884">
        <property type="term" value="F:carbon-nitrogen ligase activity, with glutamine as amido-N-donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019004">
        <property type="entry name" value="YqeY/Aim41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042184">
        <property type="entry name" value="YqeY/Aim41_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28055">
        <property type="entry name" value="PTHR28055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09424">
        <property type="entry name" value="YqeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="145" mass="16752" checksum="4A313F4090EB7CB3" modified="2009-02-10" version="1">MLKEKIMQDLKQAMKEKNEIKVRVLRLLNSAIKNFEVEKIGEASDEEIEKLVLKEMKKRQESIEAYKKAGREDLAKEEEQELEVLKGYAPEMLSEDEIRKMVKEIIEELNATQKEFGLVMKNLMAKVKGKADGSVVSKIVKELLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDF9</accession>
    <name>B7IDF9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1598</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580286.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1598"/>
    <dbReference type="EnsemblBacteria" id="ACJ76036">
        <property type="protein sequence ID" value="ACJ76036"/>
        <property type="gene ID" value="THA_1598"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1598"/>
    <dbReference type="eggNOG" id="ENOG502ZUIT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144857_0_0_0"/>
    <dbReference type="OrthoDB" id="2126089at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002857303" evidence="1">
        <location>
            <begin position="21"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="153" mass="17112" checksum="CA3340995F25B42B" modified="2009-02-10" version="1">MRKMLITLLSLFALFIFADATSVHVPGCDWEEYTIDSSITIFVHQWMDLDYEWLIADFDICDYTQTGDVDVLTFTLDSNAPVNVIPYADYNGLDAYIDADVYVTKDGNPFVPTSPIDDGVYLIGFTINNIDPDLPAGDYPVVLSLTFVPTVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDI6</accession>
    <name>B7IDI6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ggdef domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580304.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1625"/>
    <dbReference type="EnsemblBacteria" id="ACJ76063">
        <property type="protein sequence ID" value="ACJ76063"/>
        <property type="gene ID" value="THA_1625"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1625"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1427385_0_0_0"/>
    <dbReference type="OMA" id="RVYSKEH"/>
    <dbReference type="OrthoDB" id="1620145at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="95"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="197" mass="23227" checksum="AD2253AAF31C7A15" modified="2009-02-10" version="1">MSEYEKMTREELIAKIKELEDEVVFLKSRQNELEGLLNEYSEIVKKQFEAFDDFIKDLGTKRMVDPLTRVYSKEHILKLISYYHQKAFEENFEYAIVMIKLLDINSKEDFEKEQIIITLGKVLREAVRVPLDSIGRYSYDSFLVLLTEITKENAETVKERIAHLIELKIPDVKFEIRMAVYPHDSTSLEELLNIVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDJ4</accession>
    <name>B7IDJ4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Basal-body rod modification protein FlgD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1633</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for flagellar hook formation. May act as a scaffolding protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the FlgD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580312.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1633"/>
    <dbReference type="EnsemblBacteria" id="ACJ76071">
        <property type="protein sequence ID" value="ACJ76071"/>
        <property type="gene ID" value="THA_1633"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1633"/>
    <dbReference type="eggNOG" id="COG1843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047535_0_1_0"/>
    <dbReference type="OMA" id="STYAHVT"/>
    <dbReference type="OrthoDB" id="709873at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005648">
        <property type="entry name" value="FlgD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025965">
        <property type="entry name" value="FlgD_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03963">
        <property type="entry name" value="FlgD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13860">
        <property type="entry name" value="FlgD_ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FlgD_ig" evidence="4">
        <location>
            <begin position="105"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="222" mass="24969" checksum="1E91C29C5E90DD1C" modified="2009-02-10" version="1">MMNWVSMDNIYNLSTNSKKQEIKKELDKEAFLELLVTQLKNQDPLEPLKDREFIAQMTQLSTLEQITNMSESVQKFVESSASLYRAQASSMVGKYAVVKSNVLELSNGTTSTQVFKLESPSHVILKIYDENGKLVRQEDLGNLSEGMQTFLWDGRSDDGVMLPDGKYTFNLYSVQPDGNLEEISAMDGGKVEAVQFRDNDVYVLVNGNLYPISSIIEISQEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-22" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDT7</accession>
    <name>RIMP_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome maturation factor RimP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rimP</name>
        <name type="ordered locus">THA_1728</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for maturation of 30S ribosomal subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RimP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102604.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDT7"/>
    <dbReference type="SMR" id="B7IDT7"/>
    <dbReference type="STRING" id="484019.THA_1728"/>
    <dbReference type="EnsemblBacteria" id="ACJ76164">
        <property type="protein sequence ID" value="ACJ76164"/>
        <property type="gene ID" value="THA_1728"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1728"/>
    <dbReference type="eggNOG" id="COG0779">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070525_2_2_0"/>
    <dbReference type="OMA" id="YIHVSLY"/>
    <dbReference type="OrthoDB" id="1595498at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01734">
        <property type="entry name" value="YlxS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01077">
        <property type="entry name" value="RimP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003728">
        <property type="entry name" value="Ribosome_maturation_RimP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028998">
        <property type="entry name" value="RimP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036847">
        <property type="entry name" value="RimP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028989">
        <property type="entry name" value="RimP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035956">
        <property type="entry name" value="RimP_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33867">
        <property type="entry name" value="PTHR33867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02576">
        <property type="entry name" value="DUF150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17384">
        <property type="entry name" value="DUF150_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74942">
        <property type="entry name" value="SSF74942"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75420">
        <property type="entry name" value="SSF75420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_0000384798" description="Ribosome maturation factor RimP">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01077"/>
        </source>
    </evidence>
    <sequence length="148" mass="17532" checksum="BD6C2725B1424B6C" modified="2009-02-10" version="1">MKEIVSKVKEIAESACKKYNLELFDIKYYNKSGKWFLEIVIDNPLDYVSTKDCENISREVEFQLDKLDIIPQRYYLTVSSPGLDRPLRSIDDFKRFINNKVKIKLENETIIGYIKDVKDSVIFLEENNKKIKEIKYNQIKKANLEIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDU8</accession>
    <name>B7IDU8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallo-beta-lactamase superfamily, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102632.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1740"/>
    <dbReference type="EnsemblBacteria" id="ACJ76175">
        <property type="protein sequence ID" value="ACJ76175"/>
        <property type="gene ID" value="THA_1740"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1740"/>
    <dbReference type="eggNOG" id="COG1234">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1000640_0_0_0"/>
    <dbReference type="OMA" id="TRNNAMG"/>
    <dbReference type="OrthoDB" id="1712770at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="21"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="279" mass="33031" checksum="596A0918F386B789" modified="2009-02-10" version="1">MNVIAYSKALYTTWIYYSPERILFDAGEGVATLLNNKIYAIKHIFLTHGHVDHISGLWALINTRNNAMGDREKELNIYYPIGNKGIEEYLRFIKKMNSDLRFNLNVFPLKDGQIVYLREGNQPKYIKAFSVVHTYSEKSFGYHLYEVRKRLKKEFQSLSQKEIAELSRKYGSDYVSEKFEKKILTVSGDTYLLKKEDVFDTDILFHECTFFKKEDRKYKNHAVLEDIIDLIKDTKIKTLILYHISGRYGKRPERFLENFKEELKNIDVHIVKPDKLFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDV3</accession>
    <name>B7IDV3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1746</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1746"/>
    <dbReference type="EnsemblBacteria" id="ACJ76180">
        <property type="protein sequence ID" value="ACJ76180"/>
        <property type="gene ID" value="THA_1746"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1746"/>
    <dbReference type="eggNOG" id="ENOG5032VSC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129785_0_0_0"/>
    <dbReference type="OMA" id="PLEFQCT"/>
    <dbReference type="OrthoDB" id="1845025at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="147" mass="16767" checksum="AD46DECE9B6B90A2" modified="2009-02-10" version="1">MIGYLSVKKIIDKYIGGILIINDKGIPVEFKYTEPIIPTAIQKIIYGSSLEEYLSTEIIGKTLIEKIENKPEIIFVDSQELLSLENVVFLAELKTPTDESLEINNRYFKIIPLKENIKGILIDISEKIDITEPFSRLEKALNYVCES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDX3</accession>
    <name>B7IDX3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sun protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1769</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580397.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1769"/>
    <dbReference type="EnsemblBacteria" id="ACJ76200">
        <property type="protein sequence ID" value="ACJ76200"/>
        <property type="gene ID" value="THA_1769"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1769"/>
    <dbReference type="eggNOG" id="COG0144">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0781">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_0_1_0"/>
    <dbReference type="OMA" id="RVNRQHH"/>
    <dbReference type="OrthoDB" id="1064993at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="6">
        <location>
            <begin position="141"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="235"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="353"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ76200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="415" mass="48897" checksum="38597DC3C0CF91B7" modified="2009-02-10" version="1">MNDLIIAYRLLRKKFRRGIFSSEFYQAFSYVEEKAFFKNLIFGVLRKQEYLDWIIDSYLKKRDIPPSIRTILRIGAYQLIFTNKPSYSVVNNCVELVEKKSFRGLVNAVLRKIAKIGYIEPKELHLKYSHPKWLIEYWKTFLPNDYLLEMLEYNQKPLKTTARVNMRKINRKDLVLENITFAPHSPVGIIFERLETNPWELEEYKNGYITFQSESSQIIPLLVNYESKENVLDACAAPGGKATHILEISDVNLYVNDYEKSKISILEEQFERLGLSPKRIMCEDASNLKLDNLFDKIFVDAPCTSLGTGRRNPEVLRRQSKNNILELSKLQKKIVSNLWKYLNKFGLLIYSTCTVTYEENTQVVKEFSRYAEFVDIREKLEKFNIKYIWDGYGALFYPDETLTPFYVSILRKVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDX7</accession>
    <name>B7IDX7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1773</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102679.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1773"/>
    <dbReference type="EnsemblBacteria" id="ACJ76204">
        <property type="protein sequence ID" value="ACJ76204"/>
        <property type="gene ID" value="THA_1773"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1773"/>
    <dbReference type="HOGENOM" id="CLU_1569933_0_0_0"/>
    <dbReference type="OMA" id="GCNDENC"/>
    <dbReference type="OrthoDB" id="1766798at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="173" mass="20010" checksum="C007BF9B378531C2" modified="2009-02-10" version="1">MKKAIIYTLLLAIFLFFWGCNYEKYNNCVSIGNEKYCLQDNDEIISINTNFGYLIGNKKNLIYFEGISQSGGVDIEDSLNKARIDAALKMIDFFGVKVGTYNGNVKINYGSISEVSLKIIKILNSSLEYLVFRRNRGNFYEIHTILIYIPGNKKFIPIYEDEFSKKLWQYANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDY6</accession>
    <name>B7IDY6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">FlaG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1782</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580407.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1782"/>
    <dbReference type="EnsemblBacteria" id="ACJ76213">
        <property type="protein sequence ID" value="ACJ76213"/>
        <property type="gene ID" value="THA_1782"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1782"/>
    <dbReference type="eggNOG" id="COG1334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120910_3_3_0"/>
    <dbReference type="OMA" id="REIFKGE"/>
    <dbReference type="OrthoDB" id="2036889at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005186">
        <property type="entry name" value="FlaG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035924">
        <property type="entry name" value="FlaG-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37166">
        <property type="entry name" value="PTHR37166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03646">
        <property type="entry name" value="FlaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160214">
        <property type="entry name" value="SSF160214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="112" mass="12859" checksum="436658C1FDFAA0BF" modified="2009-02-10" version="1">MDGIKGVENRRVDDQIIYSDRNVKTSDNVQQRNKEKVDPEFAASIFKENLEKLKVIFNAEAEFKIDQETGMIIVKIKEKDTGEIIRQIPPEVALKLAKNIEELLGMVFDEHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE04</accession>
    <name>B7IE04_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">AP endonuclease, family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580425.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1800"/>
    <dbReference type="EnsemblBacteria" id="ACJ76231">
        <property type="protein sequence ID" value="ACJ76231"/>
        <property type="gene ID" value="THA_1800"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1800"/>
    <dbReference type="eggNOG" id="COG1082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1151359_0_0_0"/>
    <dbReference type="OMA" id="VVHFPNA"/>
    <dbReference type="OrthoDB" id="1007505at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="241" mass="28783" checksum="790AF7F989B5C12F" modified="2009-02-10" version="1">MQRLGVSTSVVKSDLRMIKYLTEKFKFSNLFEIGFIDPKYINNLNVIKGKSIGVHSPFIFKLKNHPKLTHSDYHFTFSKILRSAYFAKQIKSEYLIVHYPDALQNDEWNKNLFLLEELNSIIKIRIENTYGNKFFYSEKDYKNLCQDLNLKLCIDIGHLLLDERINPFKFIEFLEEYIEEFHIYYADKKIYKICHHKPWSENKYYIELLKLIMKLDADIVIESTPQCNKDLDKLLKFLGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE08</accession>
    <name>B7IE08_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Biotin synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_1804</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1804"/>
    <dbReference type="EnsemblBacteria" id="ACJ76235">
        <property type="protein sequence ID" value="ACJ76235"/>
        <property type="gene ID" value="THA_1804"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1804"/>
    <dbReference type="eggNOG" id="COG0502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033172_0_1_0"/>
    <dbReference type="OMA" id="IMGFNPY"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044271">
        <property type="term" value="P:cellular nitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018130">
        <property type="term" value="P:heterocycle biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901362">
        <property type="term" value="P:organic cyclic compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901566">
        <property type="term" value="P:organonitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042364">
        <property type="term" value="P:water-soluble vitamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024021">
        <property type="entry name" value="FeFe-hyd_HydE_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034422">
        <property type="entry name" value="HydE/PylB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43726">
        <property type="entry name" value="PTHR43726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00348">
        <property type="entry name" value="FeFe_hydrogenase_maturase_(Hyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03956">
        <property type="entry name" value="rSAM_HydE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="45"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ76235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="341" mass="38961" checksum="0AEBF12A58B55DB1" modified="2009-02-10" version="1">MHEIIKKLRKDSLTIEELAHIIENEKYDEEILNYANEIREKYVGNEVHVRAIIEFSNYCRMDCLYCGLRAPNKNIKRYRMKIDEIIDRAKLIAERGIKTVVLQSGEDTYYTKEIMSNLIKEIKKFDVAITLSIGEREFDEYKAWKEAGADRYLMRHETADEALYNKLHPSDTFENRKRHLFKLKELGYEVGAGCMVGLPGQGPIELAKDLIFLRDLDSDMIGIGPFIPNPDTPLKDEKGGDLKTTLKIIALARILIPTANIPATTAMGSLHPFGRQLALKYGANVIMPNLTPNPYRPNYTLYPNKICLFEADTSCIECTRAMIKSLNREVGKSYGFRVKAS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE29</accession>
    <name>TAL_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable transaldolase</fullName>
            <ecNumber evidence="1">2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tal</name>
        <name type="ordered locus">THA_1825</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = beta-D-fructose 6-phosphate + D-erythrose 4-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transaldolase family. Type 3B subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580448.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IE29"/>
    <dbReference type="SMR" id="B7IE29"/>
    <dbReference type="STRING" id="484019.THA_1825"/>
    <dbReference type="PRIDE" id="B7IE29"/>
    <dbReference type="EnsemblBacteria" id="ACJ76256">
        <property type="protein sequence ID" value="ACJ76256"/>
        <property type="gene ID" value="THA_1825"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1825"/>
    <dbReference type="eggNOG" id="COG0176">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079764_0_0_0"/>
    <dbReference type="OMA" id="VRHPMHV"/>
    <dbReference type="OrthoDB" id="784333at2"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00956">
        <property type="entry name" value="Transaldolase_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00494">
        <property type="entry name" value="Transaldolase_3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022999">
        <property type="entry name" value="Transaldolase_3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004731">
        <property type="entry name" value="Transaldolase_3B/F6P_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033919">
        <property type="entry name" value="TSA/FSA_arc/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="PTHR10683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00875">
        <property type="entry name" value="fsa_talC_mipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00958">
        <property type="entry name" value="TRANSALDOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0570">Pentose shunt</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000126365" description="Probable transaldolase">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00494"/>
        </source>
    </evidence>
    <sequence length="216" mass="23932" checksum="881BDD620C61F7E9" modified="2009-02-10" version="1">MKIFLDTANLDEIRRGVEWGVVDGVTTNPTLISKEGKPFEETIKEICNIVQGPISAEVISLNFEGMIDEARNLAKIDENIVIKIPMTPNGIKAVKVLSKEGIKTNVTLIFSPNQALLAAKAGATYVSPFVGRVDDLANDGLKIVEEIMQIYENYGFETEVIVASVRHPMHVLEAALIGADIATVPFSVLEKMFKHPMTDIGIERFLKDWEKYKTGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE34</accession>
    <name>DER_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTPase Der</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP-binding protein EngA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">der</name>
        <name type="synonym">engA</name>
        <name type="ordered locus">THA_1830</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">GTPase that plays an essential role in the late steps of ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngA (Der) GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580451.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IE34"/>
    <dbReference type="SMR" id="B7IE34"/>
    <dbReference type="STRING" id="484019.THA_1830"/>
    <dbReference type="EnsemblBacteria" id="ACJ76261">
        <property type="protein sequence ID" value="ACJ76261"/>
        <property type="gene ID" value="THA_1830"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1830"/>
    <dbReference type="eggNOG" id="COG1160">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016077_6_2_0"/>
    <dbReference type="OMA" id="KFRFLEY"/>
    <dbReference type="OrthoDB" id="263682at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00195">
        <property type="entry name" value="GTPase_Der"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031166">
        <property type="entry name" value="G_ENGA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016484">
        <property type="entry name" value="GTP-bd_EngA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032859">
        <property type="entry name" value="KH_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14714">
        <property type="entry name" value="KH_dom-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006485">
        <property type="entry name" value="GTP-binding_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03594">
        <property type="entry name" value="GTPase_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51712">
        <property type="entry name" value="G_ENGA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_1000118652" description="GTPase Der">
        <location>
            <begin position="1"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="EngA-type G 1">
        <location>
            <begin position="2"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="EngA-type G 2">
        <location>
            <begin position="182"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="KH-like" evidence="1">
        <location>
            <begin position="359"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="55"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="118"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="188"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="235"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="301"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00195"/>
        </source>
    </evidence>
    <sequence length="439" mass="49676" checksum="E0C902C38C49D32C" modified="2009-02-10" version="1">MPTVLIVGKSNVGKSTLFNKLIGYKKSIVDDKEGVTRDAVSGRVSYYSKTFNIVDTGGIFENPEDDIINKSKDLTLKMLNEADLILFVIDAKNGITSEDYYLADIIRKSNNDVILVSNKSESERRVQNNLPDIYKLGFGDPIFVSAEQGKNIDTLIERIVNVLESKGLSLEENLKEESQSIIRVSFIGRPNAGKSTLFNSILNKERVLVTPIPGTTRDSVDELVTINGRKYLFVDTAGLRRKSKVDYKSLDMYSNVRSIKSIENSDVVVILIDAIEGITHQDQRIAGIAENRGKATIVAFNKIDLIKNFKYKKEEFIDQFNEKLYFINYSPLIFLSAINKKGIDNLINAIDEAYKSLHYRVQTSAVNSAIQRMMAFTPPPKGLKILYGTQVDIRPPTFLFFTNGKKVPEFYQNHIRKTIRENIYPFVGAPIFLKFKSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE46</accession>
    <name>B7IE46_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Respons regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1842</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102811.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1842"/>
    <dbReference type="EnsemblBacteria" id="ACJ76273">
        <property type="protein sequence ID" value="ACJ76273"/>
        <property type="gene ID" value="THA_1842"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1842"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_628413_0_0_0"/>
    <dbReference type="OMA" id="HSGANKF"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="3"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="323"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="463" mass="54118" checksum="F3B590B0A891E1BD" modified="2009-02-10" version="1">MKKILLVDDSKFWRLLLYDLLKSNFEVYIADSGFDGIKKAFEYYPDIILTDYNMPDISGIFLSIVLRNYDEFKNSGIFILTSSDDKLNDFWVKKSGANKFFSKSIFKEKDKLDDFFITLKMKDFYSIKTNNHKISREKIYEIIEQKLKSEIFQKEILNLLKYVRDEKHLVFRLYSFFKDLFDVDSFFALLLSESEGRIYSFNGNVSKNHAKALLLSYFEKPTIPTTWTFIGDFNDNSSKKIKEHEKFLIKFENKEEGIILVEPRLSNFKSMTDLISTSLGVVFNTLNNFKEYINASIFDGLTELYNKKNLIIKIEEFVKKYSTDSTIAIIDIDNFKSVNDKYGHVVGDKVLKKLAEIIKNSIDKNAIAGRYGGEEFIILFNSPDVAFTSIEKIFDTIHKTDWGDILNDKRKITISCGVSSYKMNQTITDFIDSADKLLYKAKKDGKDRYYFENYNLMENKYIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE64</accession>
    <name>B7IE64_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pega domain family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1865"/>
    <dbReference type="EnsemblBacteria" id="ACJ76291">
        <property type="protein sequence ID" value="ACJ76291"/>
        <property type="gene ID" value="THA_1865"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1865"/>
    <dbReference type="eggNOG" id="ENOG5033U4T">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_598430_0_0_0"/>
    <dbReference type="OMA" id="YGYPENA"/>
    <dbReference type="OrthoDB" id="914823at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013229">
        <property type="entry name" value="PEGA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08308">
        <property type="entry name" value="PEGA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PEGA" evidence="1">
        <location>
            <begin position="87"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="PEGA" evidence="1">
        <location>
            <begin position="220"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="458" mass="51602" checksum="EFF223E13345D3CF" modified="2009-02-10" version="1">MKKFAVFVMFFVSMFLFANLTIITEPSAMVYWNGALIDVVPLDGVLELENLDYPGTLKIVKPGYAPYETLVSTDTILKVKLSLPSYIEVTSDPENVDVIVNGNFLGTSPDVFEVSSGELLLTFEKEGYISKKFKITLLPSQIKKIHVTLTKNVKLKLISNDVIEATIDGKYIKLPTTVEVLPGKHVLKLYGKDYIKTVQEIEVPAVEEYEYNVDETVYVNLYVYGYPENANVILNNISKTSPAIFKVIPGIYDIKIESDGFKTLKEKINVTTSSNTFMYNLSKNLISSVNNSLTVFFDGYISKDNLVPKRLYFTKVVGDNKEWFGFTDGSIEEIPKSFSVIFTKDGYIEYKGVRYNSPVILNVNYDDTVKVVSNGKEESVIVKENKILDDPKSCAVNIYSKQVFDVKINGKFIGRTPIYLLSLPEGGYRMEFLKNGITIYEKNVEIKQGILNEIYGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE71</accession>
    <name>B7IE71_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal phosphate homeostasis protein</fullName>
            <shortName evidence="1">PLP homeostasis protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1872</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Pyridoxal 5'-phosphate (PLP)-binding protein, which is involved in PLP homeostasis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102870.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1872"/>
    <dbReference type="EnsemblBacteria" id="ACJ76298">
        <property type="protein sequence ID" value="ACJ76298"/>
        <property type="gene ID" value="THA_1872"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1872"/>
    <dbReference type="eggNOG" id="COG0325">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059988_1_0_0"/>
    <dbReference type="OMA" id="IEWHMIG"/>
    <dbReference type="OrthoDB" id="1423012at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02087">
        <property type="entry name" value="PLP_homeostasis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011078">
        <property type="entry name" value="PyrdxlP_homeostasis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146">
        <property type="entry name" value="PTHR10146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004848">
        <property type="entry name" value="YBL036c_PLPDEIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00044">
        <property type="entry name" value="TIGR00044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01211">
        <property type="entry name" value="UPF0001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ala_racemase_N" evidence="4">
        <location>
            <begin position="13"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1 2">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR004848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="227" mass="26064" checksum="5BA59FB927AC1879" modified="2009-02-10" version="1">MSEIYKRYMEVVDSIKNKCKQIDRDYSKIKLVAVSKTFPVEVLKEAYDSGINIFGENYAQELRDKSKVLKDYNIEWHFIGRIQINKLKYIVPVASLIHSVSRIEEIEVIDKISKKLGKIQEILIQVNVSGEETKSGVKPEQLIDLIEKSKLYENVKVIGLMTMAPFTDNEEIIRNVFKKARVLRDNVSKEFPDVVELSMGMSNDYLIALEEGATILRIGSKIFGSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE78</accession>
    <name>B7IE78_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1879</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102878.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1879"/>
    <dbReference type="EnsemblBacteria" id="ACJ76305">
        <property type="protein sequence ID" value="ACJ76305"/>
        <property type="gene ID" value="THA_1879"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1879"/>
    <dbReference type="eggNOG" id="ENOG5033G8M">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1371644_0_0_0"/>
    <dbReference type="OMA" id="YSSMITE"/>
    <dbReference type="OrthoDB" id="1630723at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="203" mass="23664" checksum="7564E4EF0D586761" modified="2009-02-10" version="1">MYGIVSDLYRNIVRLKTNNGDIVIKSNKKMPKGLKVEIKNIGQGDYKGKILMGPSKVLPSLDAIYYSSMITEDRSLVEKLSFLFEELSKRVKIDRSFLEKFKKYFEAGEVDEDNKVFGNYVNLLSGRYGFRSFGMIKIFVDRKAEEFVLYFKDNVIKGKVEGNDIFLSTDKIIENIEQLKERLKKYFLNVYIKYENFEGGIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEG0</accession>
    <name>B7IEG0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, LacI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1966</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580513.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1966"/>
    <dbReference type="EnsemblBacteria" id="ACJ76387">
        <property type="protein sequence ID" value="ACJ76387"/>
        <property type="gene ID" value="THA_1966"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1966"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_0"/>
    <dbReference type="OMA" id="EAPYDHI"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00036">
        <property type="entry name" value="HTHLACI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="324" mass="36887" checksum="7CC6D0E478F336DE" modified="2009-02-10" version="1">MVTIEDVAKKAGVSIATVSRVLNNANNVSEKTKLKVWKAIEELNYKPKILASALARHKEKFRVGICESKRLIDMEKEKVVPEFYTVILTALEKTGEAYGIKFCKMDLFNPSECDGYILVGGDTTKELIKEYKNLKKPFLLLDHYIPGEKVDCIVTNGFDGAYFATKYLIKKGLRKIYHIHGPLNSYGFKGRFDGYTSAMEEAGLLPKYYEYDDVKDNMSDVIDFMLRKNERPEAIFASNDITAMRVIRELKKRNIKVPKDVSVIGFDDIISAESFDPPLTTLKVFKDEMGSLAAKRIYELLVGQDLHPIMMSLFTKFIKRKSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEH5</accession>
    <name>B7IEH5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1981</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ76402">
        <property type="protein sequence ID" value="ACJ76402"/>
        <property type="gene ID" value="THA_1981"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1981"/>
    <dbReference type="HOGENOM" id="CLU_2720971_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="72" mass="8591" checksum="AE75EF636D3981E4" modified="2009-02-10" version="1">MKRLSKKKVSKKLKRLFSTLLVIDSSEIRGKKKRLHLLYEANAKVLLDFEIGNNREVEHEEIMLEDMKVRYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEL3</accession>
    <name>B7IEL3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydrolase_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_19</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_19"/>
    <dbReference type="EnsemblBacteria" id="ACJ74527">
        <property type="protein sequence ID" value="ACJ74527"/>
        <property type="gene ID" value="THA_19"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_19"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071528_0_0_0"/>
    <dbReference type="OMA" id="HYDPISY"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="50"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="309" mass="36511" checksum="E3771A12543EBA74" modified="2009-02-10" version="1">MIYEYDKTIPFNLEIEKYENYSLLKFDTVYEPDIPESKKIYVYKYNSENPLGRLIFLHGIGNGHIPYLMWFAEKFSKMGLETYFLIHPYHQKRAKTDWEGGEPFYHHSPAHCVVRFHQAVKDVRRTIDLIEEIENKLPIGIMGFSFGGMIATISMALDKRIKAGVLSFTGGDWRWINWYSPYLEPVREGYKKYSNEWGCKDETKCVKLREESFNKLNELKNINEIFSLKPGCFHYDPISYAKFVDQPVLFFKGMFDKIMPKNASNNLYNKLQHARKVTIPSGHKSSYFFRRVIARKSLHFFKNTLNKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEL7</accession>
    <name>B7IEL7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_23</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103332.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_23"/>
    <dbReference type="EnsemblBacteria" id="ACJ74531">
        <property type="protein sequence ID" value="ACJ74531"/>
        <property type="gene ID" value="THA_23"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_23"/>
    <dbReference type="HOGENOM" id="CLU_2439783_0_0_0"/>
    <dbReference type="OrthoDB" id="2222425at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="90" mass="10317" checksum="4260B340C48CEFEF" modified="2009-02-10" version="1">MAISVQAKRSRVEKLSKLSVLELLKKFFLVAIPALLIFTMVYVVVHFSTLNVEIQTQKEAYQRELVELNTKLINLNKSIESLTIGLENIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEM3</accession>
    <name>B7IEM3_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_29</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Bacterial flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579303.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_29"/>
    <dbReference type="EnsemblBacteria" id="ACJ74537">
        <property type="protein sequence ID" value="ACJ74537"/>
        <property type="gene ID" value="THA_29"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_29"/>
    <dbReference type="eggNOG" id="COG1344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011142_2_0_0"/>
    <dbReference type="OMA" id="ENEKRMN"/>
    <dbReference type="OrthoDB" id="636786at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001492">
        <property type="entry name" value="Flagellin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046358">
        <property type="entry name" value="Flagellin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042187">
        <property type="entry name" value="Flagellin_C_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001029">
        <property type="entry name" value="Flagellin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42792">
        <property type="entry name" value="PTHR42792"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00700">
        <property type="entry name" value="Flagellin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00669">
        <property type="entry name" value="Flagellin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00207">
        <property type="entry name" value="FLAGELLIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Flagellin_N" evidence="4">
        <location>
            <begin position="3"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Flagellin_C" evidence="5">
        <location>
            <begin position="301"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="388" mass="42017" checksum="49699584F99EC966" modified="2009-02-10" version="1">MRINHNLSALNAWRQISLTNSNMSKTLEKLSSGLRINRAADDAAGLAISEKMRGQIKGLDMARKNAQDAISLIQTAEGALNEVHSILQRMRELAVQAASDTNTDVDRENIQAEIEQLRQEIDRIARSTEFNTKKLLNGNLESFRANPEANIVNGGIIDLTINTLNQNAKEGTYLVEVGQFRGSPTSALDVRITLVTTSSVYSTIVTSFAQGYVLVGGVTLRWDSTVLSISNYNGLPSGEVIDAAVARIEGKYTGSDYLVFQIGANEGANMIGGIDAVDSKNLGLNTSLIDVTSQDGAERVISLIDAAIDKVSGIRSKLGAMQNRLEHTIANLGVSSENLTSSESRIRDADMAKQMMFFTKQQILIQSGMAMLAQANTLPQNILQLLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEM5</accession>
    <name>B7IEM5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_31</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579305.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_31"/>
    <dbReference type="EnsemblBacteria" id="ACJ74539">
        <property type="protein sequence ID" value="ACJ74539"/>
        <property type="gene ID" value="THA_31"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_31"/>
    <dbReference type="eggNOG" id="COG0444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_0"/>
    <dbReference type="OMA" id="YEPAHPY"/>
    <dbReference type="OrthoDB" id="1101128at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="7"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="43"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="338" mass="38240" checksum="5686EE39FED72610" modified="2009-02-10" version="1">MEKKPLLEVKNLKTYFHTEDGIVKAVDGVDFEVYPGETLGIVGESGSGKSVTSLSILRLLDPNGEILEGSQIIFEGKNLLELSEDEMRKIRGNDIAMIFQEPMVALNPVFTIGEQIMEAILLHQDVDKKEARRMAIDMLRKVGIPEPEKRVDEYPHELSGGMRQRAMIAMALSCKPKLLIADEPTTALDVTIQAQILDLMKELQREYGMAIILITHDVGVIAENADRVVVMYGGMVMETADVKELFKEMRHPYTWGLLNSIPRLDIEQDKLYNIPGIVPDPLHFPPGCRFNTRCEFADERCKKEIPPLYEVGKNHYSRCFYYKKVEEAQKLKKAGESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEP1</accession>
    <name>B7IEP1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_47</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579315.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_47"/>
    <dbReference type="EnsemblBacteria" id="ACJ74555">
        <property type="protein sequence ID" value="ACJ74555"/>
        <property type="gene ID" value="THA_47"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_47"/>
    <dbReference type="eggNOG" id="ENOG503310D">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072793_0_0_0"/>
    <dbReference type="OMA" id="IVNIPWD"/>
    <dbReference type="OrthoDB" id="1637426at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="313" mass="36755" checksum="2A05B82BBFAE7B1D" modified="2009-02-10" version="1">MPNLLSRRLVGFDIIGSKVYQAVGEYFFGKIKIKELREYNGDFEFEVDDLIIVNIPWDLILNLNLQMPMIKKRKDIENYIVLEVSQNFNLESSEFSFDFLKTSNGLEVFVVRKADLNEYFHKLESMKIPEPDIVYPDFFKESLLLSKFPGYNIEFFINSEYSGLIILNGSNIESVRYSELSLKYLNDLCNDEFGYNLYEVEDLDDEVLKEDVSRFLYNFLGDMFSLIEREIFVSLNSSQKQLTIEDIQSGFVYTDSELVNKLIFEHYTDSEIFNKIFTARNFKYPVIKPKYLGSLGLITRGGFEFGKFKFIQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEP2</accession>
    <name>B7IEP2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_48</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103374.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_48"/>
    <dbReference type="EnsemblBacteria" id="ACJ74556">
        <property type="protein sequence ID" value="ACJ74556"/>
        <property type="gene ID" value="THA_48"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_48"/>
    <dbReference type="HOGENOM" id="CLU_2072038_0_0_0"/>
    <dbReference type="OMA" id="FKTIVYC"/>
    <dbReference type="OrthoDB" id="2143092at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="118" mass="13711" checksum="B34A02577A695377" modified="2009-02-10" version="1">MTYIEVLMVIAISLIVFSVSIFSLFSILDKVMLEEELFKTIVYCGLWKSNADMIEYNADTVKIYYSLFTKNEILIRKKIYFGDYFEDNNLRIIPRKSVTSGTFGSFKIEPISFDVSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEP3</accession>
    <name>B7IEP3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Type II secretion system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_49</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GSP F family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579316.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_49"/>
    <dbReference type="EnsemblBacteria" id="ACJ74557">
        <property type="protein sequence ID" value="ACJ74557"/>
        <property type="gene ID" value="THA_49"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_49"/>
    <dbReference type="eggNOG" id="COG1459">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035032_2_1_0"/>
    <dbReference type="OMA" id="TRQMATM"/>
    <dbReference type="OrthoDB" id="1268170at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003004">
        <property type="entry name" value="GspF/PilC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30012">
        <property type="entry name" value="PTHR30012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00812">
        <property type="entry name" value="BCTERIALGSPF"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="8">
        <location>
            <begin position="65"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="8">
        <location>
            <begin position="266"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="398" mass="45083" checksum="0C14284927695AB0" modified="2009-02-10" version="1">MKFAYKAYDKEMKKIRGEIEAYNLQEALSKLKSSGYIVIDIKEASKVKKFNFNIFSIRLKDIVFFTRQLETMVKAGLRLREAIYILSNQEIFSKRFRSILVNIVSELDVGTSLSEAFKKQKAFDDVFINMIKAGEEGGVLEETLEKLASYYENLNRTNQQVKSAMVYPTFILGFAVIVVVVISVFILPKLFNVFGTIPTGGIISFLISVNKVVTGNPFMVAFVIISLLVGMFLFLRTEPGKKTKEFFGRLFPLIRKLQEKITYERFCRTFGVLIASGVSIIDSLEMAAKASNNLKFIMKIKNVSEKVKSGSTLKQALKSEKVFPQIIYEMIGTGEETGKLDTIMEKVADFFDEQIQQDTKKLLSMLEPLMIAFVGLFIAFLAYAMYSTIFQMQSTFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEQ2</accession>
    <name>B7IEQ2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Signal peptidase I</fullName>
            <ecNumber evidence="3 5">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">lepB</name>
        <name evidence="7" type="ordered locus">THA_58</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the peptidase S26 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="3 5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579322.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_58"/>
    <dbReference type="EnsemblBacteria" id="ACJ74566">
        <property type="protein sequence ID" value="ACJ74566"/>
        <property type="gene ID" value="THA_58"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_58"/>
    <dbReference type="eggNOG" id="COG0681">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028723_5_1_0"/>
    <dbReference type="OMA" id="IFENPSY"/>
    <dbReference type="OrthoDB" id="1741894at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43390">
        <property type="entry name" value="PTHR43390"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S26" evidence="6">
        <location>
            <begin position="11"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="290" mass="33731" checksum="69751CE2CE9FDF2E" modified="2009-02-10" version="1">MKKSPKEIAKEVVITLLYAIVAATIIRLFVFETMLVPTGSMIPTINIGDRLFIEKITYQAREPEIGEIVVFWTPFRDERAEQMLRAFDKFMDLFAPSKFKGHVKYVKRLVAKEGDIITLKNVDGNWKLFVNGKVPENLKNVNYQPDGIFKYPNLWKYLDQASRLRDNKTEYRNFLYNIALKNGAELANTIFSIIGGMYPVPYGIPYHEYVDEYLKPKNVDFDDYVWTENGQVYIKIPEGFYFFMGDNSKESLDSRYFGFVPKEAVIGRPILRIWPFQSFGPVQPLPKLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IES5</accession>
    <name>B7IES5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_81</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579338.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_81"/>
    <dbReference type="EnsemblBacteria" id="ACJ74589">
        <property type="protein sequence ID" value="ACJ74589"/>
        <property type="gene ID" value="THA_81"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_81"/>
    <dbReference type="HOGENOM" id="CLU_121334_2_4_0"/>
    <dbReference type="OMA" id="MLVGNIC"/>
    <dbReference type="OrthoDB" id="2080391at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005451">
        <property type="term" value="F:monovalent cation:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098662">
        <property type="term" value="P:inorganic cation transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005133">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03334">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="85" mass="9468" checksum="C4182C78BBA38940" modified="2009-02-10" version="1">MIIIYLGTLIMLIGNFLAFFQKNILKKIHYIGAGDTSGAILIMIGLLTKNYEIPKILTTILILIVGLPASSYFISISIIRKEKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IES8</accession>
    <name>B7IES8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Manganese-dependent transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_84</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtxR/MntR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_84"/>
    <dbReference type="EnsemblBacteria" id="ACJ74592">
        <property type="protein sequence ID" value="ACJ74592"/>
        <property type="gene ID" value="THA_84"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_84"/>
    <dbReference type="eggNOG" id="COG1321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069532_3_0_0"/>
    <dbReference type="OMA" id="THCPHIT"/>
    <dbReference type="OrthoDB" id="2047422at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="SSF47979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="5">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Fe_dep_repr_C" evidence="6">
        <location>
            <begin position="68"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="161" mass="18583" checksum="01CE21B62FA77657" modified="2009-02-10" version="1">MSVEYNLTKAERKYLLMVFLTLNSMGWTRLKKIADYAGVKMPSAKQALDSLAEKGLIYYEKRGAITLTDEGKKIAIIENKNIRAVQKFLVEILGIDPEKAWDSCLKIYFDMDEEAASKFLLFAKFMNECPQEKPFFIKHFKEYLSKGKITDNCPFLNKEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IET4</accession>
    <name>B7IET4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Corrinoid adenosyltransferase</fullName>
            <ecNumber evidence="4">2.5.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Cob(II)alamin adenosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Cob(II)yrinic acid a,c-diamide adenosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Cobinamide/cobalamin adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_90</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>2 ATP + 2 cob(II)alamin + reduced [electron-transfer flavoprotein] = 2 adenosylcob(III)alamin + 3 H(+) + oxidized [electron-transfer flavoprotein] + 2 triphosphate</text>
            <dbReference type="Rhea" id="RHEA:28671"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16304"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="2.5.1.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>2 ATP + 2 cob(II)yrinate a,c diamide + reduced [electron-transfer flavoprotein] = 2 adenosylcob(III)yrinate a,c-diamide + 3 H(+) + oxidized [electron-transfer flavoprotein] + 2 triphosphate</text>
            <dbReference type="Rhea" id="RHEA:11528"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:58503"/>
            <dbReference type="ChEBI" id="CHEBI:58537"/>
            <dbReference type="EC" id="2.5.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 2/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Cob(I)alamin adenosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.17" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103449.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_90"/>
    <dbReference type="EnsemblBacteria" id="ACJ74598">
        <property type="protein sequence ID" value="ACJ74598"/>
        <property type="gene ID" value="THA_90"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_90"/>
    <dbReference type="eggNOG" id="COG2096">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083486_0_1_0"/>
    <dbReference type="OMA" id="HQACTVV"/>
    <dbReference type="OrthoDB" id="1401910at2"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00233"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:cob(I)yrinic acid a,c-diamide adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016030">
        <property type="entry name" value="CblAdoTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036451">
        <property type="entry name" value="CblAdoTrfase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029499">
        <property type="entry name" value="PduO-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213">
        <property type="entry name" value="PTHR12213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01923">
        <property type="entry name" value="Cob_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89028">
        <property type="entry name" value="SSF89028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00636">
        <property type="entry name" value="PduO_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cob_adeno_trans" evidence="5">
        <location>
            <begin position="2"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="172" mass="19894" checksum="BAD479BC70740D71" modified="2009-02-10" version="1">MISTKQGDNGKTKLANNETVYKDDLHVEAYGTVDELNSYLGYAKHFLNKKEKEIIEDIQKDLFRVATELAKGEKFINLISKEDEEKITKLVEEYEKNVNLNSFVIPGATKESSILDICRTIARRAERRIVSLSKSENVRKELIAYINRISDLLYIMARYIEKDNIMPYSAHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEV9</accession>
    <name>B7IEV9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0273 protein THA_115</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_115</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0273 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103511.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_115"/>
    <dbReference type="EnsemblBacteria" id="ACJ74623">
        <property type="protein sequence ID" value="ACJ74623"/>
        <property type="gene ID" value="THA_115"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_115"/>
    <dbReference type="eggNOG" id="COG0467">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023669_2_0_0"/>
    <dbReference type="OMA" id="FSQQFLW"/>
    <dbReference type="OrthoDB" id="856984at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01076">
        <property type="entry name" value="UPF0273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022475">
        <property type="entry name" value="UPF0273_KaiC-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03877">
        <property type="entry name" value="thermo_KaiC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KaiC" evidence="2">
        <location>
            <begin position="2"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="234" mass="26063" checksum="964F45984379225B" modified="2009-02-10" version="1">MKKVSTGIIGLDEILNGGIPEKNVVLLSGGPGTGKSIFSQQFLWHGLQNNEPGIYVALEEHPIQVRQNMSQFGWNIKPFEEKGLFALIDAFTAGFGKSKEFEKYIVRDLNDLHDFIDVLRQAIKDINAKRVVIDSVTTLYLNKPTYARSIILQLKRVLAGTGCTSLLVSQVSIDERGFGGPGVEHGVDGIIRLDLDEIKGELKRSLLIWKMRGTSHSLKRHYFEITQSGIKIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEZ0</accession>
    <name>B7IEZ0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579374.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_146"/>
    <dbReference type="EnsemblBacteria" id="ACJ74654">
        <property type="protein sequence ID" value="ACJ74654"/>
        <property type="gene ID" value="THA_146"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_146"/>
    <dbReference type="eggNOG" id="COG0793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_764898_0_0_0"/>
    <dbReference type="OrthoDB" id="2121837at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="362" mass="43305" checksum="5BBCAD60ED14862B" modified="2009-02-10" version="1">MIKKFINFTLFFVLGVVIFIVISPYVFKYQKFLPKTLNKEIELINSKLKLHYIDDERVINFKDIKISRWMNKYEFYKILVKEISKYPGVYVLLPESNKSTYVLPFEIRFVDGKFVVINSSCSIEEGAVVSKINNRNLIEYLSDFYLEKNIFNENKQFFSRYIFPFLGEFLRKKRIEVEYEYLGKSEKTIVETIPYEEFIRKEPVLIEKKSNDFADIKIFSFNFLGDKFSEIFEEFENIAKNDDIKHITLDFSYAYDIPIDLSSLYMIMSFLIDKKTTLFDEAMFKFGSYKYTYKNFGELVPNNVTFKNKEIEIVENCSDSIGRLICNIIKNEKLEFYNNYKEIITPWTRLRIYIPSAKFFIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEZ2</accession>
    <name>TMCAL_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Met) cytidine acetate ligase</fullName>
            <ecNumber evidence="1">6.3.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tmcAL</name>
        <name type="ordered locus">THA_149</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of elongator tRNA(Met), using acetate and ATP as substrates. First activates an acetate ion to form acetyladenylate (Ac-AMP) and then transfers the acetyl group to tRNA to form ac(4)C34.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + cytidine(34) in elongator tRNA(Met) = AMP + diphosphate + N(4)-acetylcytidine(34) in elongator tRNA(Met)</text>
            <dbReference type="Rhea" id="RHEA:58144"/>
            <dbReference type="Rhea" id="RHEA-COMP:10693"/>
            <dbReference type="Rhea" id="RHEA-COMP:10694"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TmcAL family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579375.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IEZ2"/>
    <dbReference type="SMR" id="B7IEZ2"/>
    <dbReference type="STRING" id="484019.THA_149"/>
    <dbReference type="PRIDE" id="B7IEZ2"/>
    <dbReference type="EnsemblBacteria" id="ACJ74656">
        <property type="protein sequence ID" value="ACJ74656"/>
        <property type="gene ID" value="THA_149"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_149"/>
    <dbReference type="eggNOG" id="COG1323">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038915_0_1_0"/>
    <dbReference type="OMA" id="WARTKMA"/>
    <dbReference type="OrthoDB" id="1547014at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01539">
        <property type="entry name" value="TmcAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008513">
        <property type="entry name" value="tRNA(Met)_cyd_acetate_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37825">
        <property type="entry name" value="PTHR37825"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HIGH_NTase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_1000146636" description="tRNA(Met) cytidine acetate ligase">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01539"/>
        </source>
    </evidence>
    <sequence length="423" mass="48763" checksum="B9C814ABA68FE53A" modified="2009-02-10" version="1">MKVLGVVVEYNPFHNGHLYHLKSAKKLVKPDFTIAVMSGNFCQRGEPAIVNKFARAKMALLNGIDVVLEIPTVYALQDAGGFAFGSVGLMHKLGLVTDIVFGSESADISFLEKISKILYENSKEFDYLIKRELKKGLSYPNARKFALQKFLKTDLDTIKKLENSNDILGIEYIKAIFKYNSNIKYHVIKRVGAKYNEQNLSGKFSSATAIRNAIKFEKNIKEYVPESTYLILKDEFEKGRGPVFLENLEQFILSEFRLKARENLENIYGFSEGLDKRFIDSANISTNLKEFIENIKAKRFTFSRIRRLIFHAIFNFEKNYMEFSNKLGPQYARVLGFTTKGQEFLSYAKKKSTIPIITNPSLKNKILKDVLKNSERKWDFNISLYNWQFEKDILASNIYTLFYPNSSQRKSGMDFRKPIIIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEZ8</accession>
    <name>B7IEZ8_THEAB</name>
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        <submittedName>
            <fullName evidence="2">Methylmalonyl-CoA mutase, N-domain/subunit</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">THA_155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579377.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_155"/>
    <dbReference type="EnsemblBacteria" id="ACJ74662">
        <property type="protein sequence ID" value="ACJ74662"/>
        <property type="gene ID" value="THA_155"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_155"/>
    <dbReference type="eggNOG" id="COG1884">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009523_5_1_0"/>
    <dbReference type="OMA" id="IAQKEWV"/>
    <dbReference type="OrthoDB" id="154460at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006099">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006098">
        <property type="entry name" value="MMCoA_mutase_a_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01642">
        <property type="entry name" value="MM_CoA_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="SSF51703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00641">
        <property type="entry name" value="acid_CoA_mut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="METMALONYL_COA_MUTASE" evidence="1">
        <location>
            <begin position="38"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="559" mass="62967" checksum="379F3AA054DAC0FD" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF06</accession>
    <name>B7IF06_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallo-beta-lactamase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Thermotogales</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
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                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579382.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_163"/>
    <dbReference type="EnsemblBacteria" id="ACJ74670">
        <property type="protein sequence ID" value="ACJ74670"/>
        <property type="gene ID" value="THA_163"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_163"/>
    <dbReference type="eggNOG" id="COG0426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066633_0_0_0"/>
    <dbReference type="OMA" id="CGIDLMT"/>
    <dbReference type="OrthoDB" id="1581636at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045761">
        <property type="entry name" value="ODP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
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        <property type="entry name" value="ODP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="29"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="254" mass="29092" checksum="9D3A770C20BA20DA" modified="2009-02-10" version="1">MNNICLYDDGKHKYYLLGWEETEDPNMVPTNQYLIVHENKGFLLDPGGAHVFPRVLANVSEIIAPENIEGIFYSHQDPDVSSGITMWLTIASRAKIYISELWIRFLPHFGVFDKNRIVGLKDRGEKGVRFSGYTLDFVPAHFLHAPGHFNVYDPISKILFSGDIGVSLLPFSQRVQIYEGDFKNLVSAMEGFHKRYMTSNAACKIWVDRVSEYEIYMIAPQHGAVLKGNVVKEFLKWFKTLKCGSDIIEQFYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF60</accession>
    <name>B7IF60_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_219"/>
    <dbReference type="EnsemblBacteria" id="ACJ74724">
        <property type="protein sequence ID" value="ACJ74724"/>
        <property type="gene ID" value="THA_219"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_219"/>
    <dbReference type="HOGENOM" id="CLU_3318375_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="39" mass="4826" checksum="96AB22AA00508436" modified="2009-02-10" version="1">MVYLIRLAAYRHWSDIRNEFTEKYIFEGLFEGKVIFKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF80</accession>
    <name>B7IF80_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579437.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_241"/>
    <dbReference type="EnsemblBacteria" id="ACJ74744">
        <property type="protein sequence ID" value="ACJ74744"/>
        <property type="gene ID" value="THA_241"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_241"/>
    <dbReference type="eggNOG" id="COG0121">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105001_0_0_0"/>
    <dbReference type="OMA" id="AFCHNGT"/>
    <dbReference type="OrthoDB" id="693464at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="1">
        <location>
            <begin position="2"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="215" mass="25328" checksum="2CE02F95ECF59427" modified="2009-02-10" version="1">MCRMLGFSFEKDKQINNLFKELQNMAKNGLKSPHSHGFGIYALTESNEVLYHKFEEPIYEKDISFPRLKIGIIHARKASPSYPIGFLQIHPFVDENGTAFCHNGTIHSLKRNNIFESDSYEYFLIVKDFKDLHDLSQRLKKFIKENEFTGINFLMIKRKKLYAFCYFNSDPDYYTMWYSKNIISSEKLGLDFKMVKKGELLIFENGKLIFKDVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFC2</accession>
    <name>B7IFC2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Laminarinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_286</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103880.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_286"/>
    <dbReference type="CAZy" id="CBM4">
        <property type="family name" value="Carbohydrate-Binding Module Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ74786">
        <property type="protein sequence ID" value="ACJ74786"/>
        <property type="gene ID" value="THA_286"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_286"/>
    <dbReference type="eggNOG" id="COG2273">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1388327_0_0_0"/>
    <dbReference type="OrthoDB" id="662473at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003305">
        <property type="entry name" value="CenC_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02018">
        <property type="entry name" value="CBM_4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="CBM-cenC" evidence="2">
        <location>
            <begin position="63"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="220" mass="25288" checksum="FCDD4C07A494F529" modified="2009-02-10" version="1">MSTNQILFYISIGLLSVLLIGGFIFNNLPAPVTSQQENAVEESTTTVVDENETTSVTPEVDLVINNGTFDEPIANDPENPYRWWIWQASDYNISNARVSSYGVKDGYAYLKVKYPGGETWHIQFNQWIKLERGATYEVSFKAKADIARKIWLNIAQNHEPWASYLPEPYILTYDLTTEWQTFTFEYTHPEDADEIVNFSFQLGLDKPTTIYFDDISIVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFC8</accession>
    <name>B7IFC8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579476.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_292"/>
    <dbReference type="EnsemblBacteria" id="ACJ74792">
        <property type="protein sequence ID" value="ACJ74792"/>
        <property type="gene ID" value="THA_292"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_292"/>
    <dbReference type="eggNOG" id="ENOG5033BMA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_762757_0_0_0"/>
    <dbReference type="OMA" id="GMHEEAM"/>
    <dbReference type="OrthoDB" id="1522512at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="369" mass="42779" checksum="F219F59A227902EB" modified="2009-02-10" version="1">MKSKAVVLILIFICIIGFSITKDEIITMSKEDPKEAWKLYLEYVLDYPTDTSLESMGKELKVKISLMNDPRLSFLIKQDIKELKNFLKNTTPSATEIEIIHQLFPQINELSKNTLEKPEYGTFLDNIIFLKITNPEIDEKISAQNLTNFFLKYPTMLTYDVINILSQKDFSKDLGYNILKYIVDNIENFEEVQYPILNRIVEFAEKIGGTFTSKYLSDLKKYVELSNSLNETSENIDINKAKLTIENLSIKKYNLSSKLENILNNTQKANIKDLSQKTSTSQNSSNNTNYIMILLLLLLAIIVVSFKLVRFYLFYILGLKKMAAKVYKKIVDKDPLNEEKHLKLAQLYEEAGMYDKALEEYNILKRIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFG5</accession>
    <name>B7IFG5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Endoglucanase M</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_329</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103969.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_329"/>
    <dbReference type="EnsemblBacteria" id="ACJ74829">
        <property type="protein sequence ID" value="ACJ74829"/>
        <property type="gene ID" value="THA_329"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_329"/>
    <dbReference type="eggNOG" id="COG1363">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047249_1_0_0"/>
    <dbReference type="OMA" id="FMAHMDE"/>
    <dbReference type="OrthoDB" id="900854at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="335" mass="36918" checksum="3B63261610330893" modified="2009-02-10" version="1">MYLKELSEINGVSGNEKKVRDFIIEKIKDKVDKFWVDRMGNLIAFKKGNGKAKVVLDAHMDEVGFMITNIEEDGSLSFMPVGGVDPRVVIGKKVIINDEIIGVIGFKAIHLQDDPYKAPEFSQLKIDAGFSSKAEAEKKVKIGDYVAFTTKYKEIGNFATGKAFDDRGGCSILIDLIESNVKSDYDLYFVFSVQEETGLRGAAVVSEQIKPDFAIALETTTAGDNPELDKSQWATHIGDGPALTFLHSGYVIDKDLFDALVDTAKKNNIPFQYKRRTAGGTNAARYARSTYGVPAAVISIPSRYIHSPIIVISLDDYKNSVLLVKKFLESAPVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFH5</accession>
    <name>B7IFH5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable butyrate kinase</fullName>
            <shortName evidence="8">BK</shortName>
            <ecNumber evidence="8">2.7.2.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Branched-chain carboxylic acid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">buk</name>
        <name evidence="10" type="ordered locus">THA_339</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + butanoate = ADP + butanoyl phosphate</text>
            <dbReference type="Rhea" id="RHEA:13585"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58079"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the acetokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.7" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579510.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_339"/>
    <dbReference type="EnsemblBacteria" id="ACJ74839">
        <property type="protein sequence ID" value="ACJ74839"/>
        <property type="gene ID" value="THA_339"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_339"/>
    <dbReference type="eggNOG" id="COG3426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048716_0_0_0"/>
    <dbReference type="OMA" id="IWHALNQ"/>
    <dbReference type="OrthoDB" id="537106at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047761">
        <property type="term" value="F:butyrate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00542">
        <property type="entry name" value="Butyrate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000890">
        <property type="entry name" value="Aliphatic_acid_kin_short-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023865">
        <property type="entry name" value="Aliphatic_acid_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011245">
        <property type="entry name" value="Butyrate_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060">
        <property type="entry name" value="PTHR21060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060:SF3">
        <property type="entry name" value="PTHR21060:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00871">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036458">
        <property type="entry name" value="Butyrate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00471">
        <property type="entry name" value="ACETATEKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02707">
        <property type="entry name" value="butyr_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01075">
        <property type="entry name" value="ACETATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01076">
        <property type="entry name" value="ACETATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="363" mass="40483" checksum="8B27EDBEF64E6033" modified="2009-02-10" version="1">MYRILVINPGSTSTKLAIFEDEKQVASKTLRHTPEELSQFKRLIDQYEFRVNVIEDFLKTLGFEYKDFNAVVGRGGLVRPIPSGTYLVDELMVEELKQAKYGEHASNLGAVIAFEISNKYNIPAYIVDPVVVDEMDKIAKISGHPMFERKSIFHALNQKAVARRAAEELGKKYEETNLIVVHMGGGISIGAHRNGKVIDVNNALDGDGPFTPERSGTLPLTQLIDLCYSGKYDYDFIKKRIKGKGGLVAYLGTNDAMKVQEMISNGDKKAELVYKAMAYQIAKWIGKMAAALKGEVDAIVLTGGLAYDENYMVKWLKEYTSFIAKVIVYPGGDEEKALAMGALRVLRGEEKARSYKEEVQMRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFK3</accession>
    <name>B7IFK3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579531.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_373"/>
    <dbReference type="EnsemblBacteria" id="ACJ74867">
        <property type="protein sequence ID" value="ACJ74867"/>
        <property type="gene ID" value="THA_373"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_373"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_18_7_0"/>
    <dbReference type="OMA" id="LEYMRMA"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="150" mass="17968" checksum="B0AC46BE19B4F9C0" modified="2009-02-10" version="1">MVDNFEFSSTFLLFSEIERLKFQILKSEMDKYKIHPGQIPIFFIVRKNPGISQKDLAKKVMLSTSTVAIMLRRMEKAGFVKRIQDESDRRFYHIYLTDKALKIVDKIFKRLKEFERQSFENFTNEEMEVLEKLLKKILLNLEAMKDERCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFK7</accession>
    <name>B7IFK7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ74871">
        <property type="protein sequence ID" value="ACJ74871"/>
        <property type="gene ID" value="THA_377"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_377"/>
    <dbReference type="HOGENOM" id="CLU_2467982_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="88" mass="10091" checksum="B134960F3533433C" modified="2009-02-10" version="1">MGEIILKEFDHGRIKNLAIEPYTKSIFISFTNNDQDMSIGYVDLKEQVVKVIDNEKVSDFLGILFNKGFLCVIKKDGYKVFSILDIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFL9</accession>
    <name>B7IFL9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleoside-diphosphate-sugar epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104117.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_390"/>
    <dbReference type="EnsemblBacteria" id="ACJ74883">
        <property type="protein sequence ID" value="ACJ74883"/>
        <property type="gene ID" value="THA_390"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_390"/>
    <dbReference type="eggNOG" id="COG1087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_0"/>
    <dbReference type="OMA" id="NLGNEDW"/>
    <dbReference type="OrthoDB" id="1180629at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="310" mass="34585" checksum="CF11E84EAFE85141" modified="2009-02-10" version="1">MKALVTGGAGFIGSHVVDKLIENGYEVVVVDNLSRGKKENVNKDATLVVADIRDEKTIEELFKEHNFSYVFHLAAQASVSVSVKDPVEDANVNIIGSLVLLKNSIKYGVKKFIFSSTGGAIYGDDVDIFPTPESVFPKPISPYGIAKFSFENYLKFANKEFGLNYTVLRYANVYGPRQDPFGEAGVVAIFTMRMLNGEDVIINGDGEYIRDYVYVEDVADANLRALKAGDGMEINIGTSQGTSVNQLFNYLKKITGYTKDPVYGPPRKGDIRKSLLCYTRALEELRWKPKVDIEKGLRLTVEWFKRNFKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFP0</accession>
    <name>B7IFP0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_411</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579559.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_411"/>
    <dbReference type="EnsemblBacteria" id="ACJ74904">
        <property type="protein sequence ID" value="ACJ74904"/>
        <property type="gene ID" value="THA_411"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_411"/>
    <dbReference type="eggNOG" id="COG0116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032119_3_1_0"/>
    <dbReference type="OMA" id="GFMYKAN"/>
    <dbReference type="OrthoDB" id="1772619at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034470">
        <property type="term" value="P:ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RNA_methylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01261">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UPF0020" evidence="2">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="344" mass="39875" checksum="C080970BE23096C2" modified="2009-02-10" version="1">MKLMITCTAGLEGATALELKNLGYKIVDSQSGRIYIEGDLKDIPRLNLFLRTAERVYIVLVQDRVETFDELYNVIYSVNWEEFVDGKVLISDVSVRNSKLSAKGAIVSVCYAAINKKILRKTNNIYPIRLIIKNNILQVLLDTTGKDALSKRGYRLKTSKAPLRETIAAALVLLSRWDKKSSLIDPFCGSGTILIEAALYKENVPPSFLRYFVSEKWSILKDYWPERKKYKVNVDNLFGFDIDKEVLKVAQENEKRANINGIIYENIDFNNLKKFENCWVVTNPPYGERLKNDINFSKLWDIFVDSKIYILSPDSNFERLVQKKARKKIRFQNSGIWVWLYMFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFQ0</accession>
    <name>B7IFQ0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Beta-alanyl-CoA:ammonia lyase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_421</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579568.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_421"/>
    <dbReference type="EnsemblBacteria" id="ACJ74914">
        <property type="protein sequence ID" value="ACJ74914"/>
        <property type="gene ID" value="THA_421"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_421"/>
    <dbReference type="eggNOG" id="COG1607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149200_0_0_0"/>
    <dbReference type="OMA" id="YAGDYIE"/>
    <dbReference type="OrthoDB" id="1712258at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="127" mass="13910" checksum="1FC29679DFE32F61" modified="2009-02-10" version="1">MTATIRVRMSTADAHYGGNLVDGAKILQLFGDVATELLIRHDGDEGLFRAYDNIEFLAPVYAGDFIEVTGEIVEVGRTSRKMVFVAKKVITARPDINDSAADVLEEPIIVCKASGTCVVPLEKQRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG05</accession>
    <name>B7IG05_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Adenylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_531</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579634.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_531"/>
    <dbReference type="EnsemblBacteria" id="ACJ75019">
        <property type="protein sequence ID" value="ACJ75019"/>
        <property type="gene ID" value="THA_531"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_531"/>
    <dbReference type="eggNOG" id="COG2114">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4252">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_85_1_0"/>
    <dbReference type="OMA" id="YYQALDP"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007890">
        <property type="entry name" value="CHASE2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05226">
        <property type="entry name" value="CHASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01080">
        <property type="entry name" value="CHASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="2">
        <location>
            <begin position="461"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="642" mass="73735" checksum="F0C7E30FAFAD2B58" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG37</accession>
    <name>B7IG37_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F-ATPase I-subunit, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579651.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_564"/>
    <dbReference type="EnsemblBacteria" id="ACJ75051">
        <property type="protein sequence ID" value="ACJ75051"/>
        <property type="gene ID" value="THA_564"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_564"/>
    <dbReference type="HOGENOM" id="CLU_1980543_0_0_0"/>
    <dbReference type="OMA" id="YYIRYIF"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="126" mass="14316" checksum="1B6A199064EB3D21" modified="2009-02-10" version="1">MKNEKIIKQFIYVIIFIALIVITIGSIITKNKTTFIVSYIFGTLGAILYIISLYYDIISLNLKRKVSKKGYYIRYIFSASIFLLVGVIFEEKLTAIISAFLGLINVKISAYLVGFLFGGGWHEKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG56</accession>
    <name>B7IG56_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative regulatory protein, FmdB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_583</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100536.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_583"/>
    <dbReference type="EnsemblBacteria" id="ACJ75070">
        <property type="protein sequence ID" value="ACJ75070"/>
        <property type="gene ID" value="THA_583"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_583"/>
    <dbReference type="eggNOG" id="COG2331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136025_3_2_0"/>
    <dbReference type="OMA" id="TYQYQCT"/>
    <dbReference type="OrthoDB" id="1914830at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013429">
        <property type="entry name" value="Regulatory_FmdB_Zinc_ribbon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09723">
        <property type="entry name" value="Zn-ribbon_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00834">
        <property type="entry name" value="CxxC_CXXC_SSSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02605">
        <property type="entry name" value="CxxC_CxxC_SSSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxxC_CXXC_SSSS" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="71" mass="7772" checksum="4B6684DFACCED633" modified="2009-02-10" version="1">MPMYRYVCKSCGHEKVELHSINDNPDISCDVCGSKMERAIGRVGIVFKGSGFYITDSRKSSSTSSTDSSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG59</accession>
    <name>B7IG59_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_586</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579661.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_586"/>
    <dbReference type="EnsemblBacteria" id="ACJ75073">
        <property type="protein sequence ID" value="ACJ75073"/>
        <property type="gene ID" value="THA_586"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_586"/>
    <dbReference type="eggNOG" id="COG0472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023982_2_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="300" mass="34695" checksum="B156306238B15679" modified="2009-02-10" version="1">MLWSFILSFLLSLISLPIIRKISIKYNITDEKNSIPYFGGLSIIFSLLLCTPFNIFQKFYIIIFGFLGIYLDLKTINYLIKLSIEALLGYLAVSQYLSNPVDLIIAILLFIFIVNSIQNKEEKRNPLSIVFLISSLFLVLTISVPFNKYFLLSISGSLIAYVIYNFSTRKIFLGNTGKYAIGSIFGISVISSLSKEYVYFFASIIVLLPYLLDTAAGIFRKIIYKKHSIENDKNNIFNKVFEKVKDRRKAVFVFLIIYLFFSLNGFYFLFNPYISFVISVLFSIFIIFKLELFKYDGDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG62</accession>
    <name>B7IG62_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endonuclease III</fullName>
            <ecNumber evidence="5">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DNA-(apurinic or apyrimidinic site) lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nth</name>
        <name evidence="7" type="ordered locus">THA_589</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579664.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_589"/>
    <dbReference type="EnsemblBacteria" id="ACJ75076">
        <property type="protein sequence ID" value="ACJ75076"/>
        <property type="gene ID" value="THA_589"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_589"/>
    <dbReference type="eggNOG" id="COG0177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_3_4_0"/>
    <dbReference type="OMA" id="WQQFTHL"/>
    <dbReference type="OrthoDB" id="1381897at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01083">
        <property type="entry name" value="nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="1 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="6">
        <location>
            <begin position="32"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="203" mass="23263" checksum="14545900266FC5BF" modified="2009-02-10" version="1">MEIEKVAKLIIENFPRDHKEKDPFKVLITTVLSQRSKDENTEIAAENLFNKYKTPFELSKAKEEDIYELIKPAGLYRQKAKRIIEISKIIVEKYSGIVPDSLEELLKLPGVGRKTANIVLYVSFSKSALAVDTHVHRISNRLGWVNTKTPEETEFKLMEILPKNLWGPINGSMVEFGKKVCKPVSPNCKICPISKYCKWEGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG81</accession>
    <name>B7IG81_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_609</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_609"/>
    <dbReference type="EnsemblBacteria" id="ACJ75095">
        <property type="protein sequence ID" value="ACJ75095"/>
        <property type="gene ID" value="THA_609"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_609"/>
    <dbReference type="HOGENOM" id="CLU_3190080_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="46" mass="5261" checksum="1085910D43645A8B" modified="2009-02-10" version="1">MYFNETIIPRISKITGNRKNESLSHKRVVSDCVVFGTYPMSSKKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGA8</accession>
    <name>B7IGA8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="8">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glycyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="8">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">glyQ</name>
        <name evidence="9" type="ordered locus">THA_644</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104481.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_644"/>
    <dbReference type="EnsemblBacteria" id="ACJ75122">
        <property type="protein sequence ID" value="ACJ75122"/>
        <property type="gene ID" value="THA_644"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_644"/>
    <dbReference type="eggNOG" id="COG0752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057066_1_0_0"/>
    <dbReference type="OMA" id="SYYQFQV"/>
    <dbReference type="OrthoDB" id="676868at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00733">
        <property type="entry name" value="GlyRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00254">
        <property type="entry name" value="Gly_tRNA_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006194">
        <property type="entry name" value="Gly-tRNA-synth_heterodimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002310">
        <property type="entry name" value="Gly-tRNA_ligase_asu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02091">
        <property type="entry name" value="tRNA-synt_2e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01044">
        <property type="entry name" value="TRNASYNTHGA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00388">
        <property type="entry name" value="glyQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50861">
        <property type="entry name" value="AA_TRNA_LIGASE_II_GLYAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="283" mass="33542" checksum="4ACBAE1B4588A909" modified="2009-02-10" version="1">MYLQDVIKKLDEFWAQNGCFIDLPYDMEMGAGTFHTSTFFGVLRNRDWKVAFVQPSRRPTDGRYGENPNRMQRFLQYQVIIKPNPENSQELYLKSLEALGINPREHDIRFVEDNWESPTLGAWGVGWEVWLDGMEITQFTYFQQVGGISLKKIPLEITYGLERIAMYLQGVDNVYDVMWNKDVKYGELFLENEKQFSIYNFEMADVEKLFKLYELYREEFNRLIENNLFLPAYEQLVKCSHTFNLLDARNAISVAQRQNYIRDIRSMAMQCAKRFLEIEEGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGB5</accession>
    <name>B7IGB5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent Clp protease ATP-binding subunit ClpX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">clpX</name>
        <name evidence="9" type="ordered locus">THA_651</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the ClpX-ClpP complex. Forms a hexameric ring that, in the presence of ATP, binds to fourteen ClpP subunits assembled into a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the ClpX chaperone family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579702.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_651"/>
    <dbReference type="EnsemblBacteria" id="ACJ75129">
        <property type="protein sequence ID" value="ACJ75129"/>
        <property type="gene ID" value="THA_651"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_651"/>
    <dbReference type="eggNOG" id="COG1219">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014218_8_2_0"/>
    <dbReference type="OMA" id="HYKRVQA"/>
    <dbReference type="OrthoDB" id="718259at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00175">
        <property type="entry name" value="ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004487">
        <property type="entry name" value="Clp_protease_ATP-bd_su_ClpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046425">
        <property type="entry name" value="ClpX_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010603">
        <property type="entry name" value="Znf_CppX_C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038366">
        <property type="entry name" value="Znf_CppX_C4_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06689">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00994">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00382">
        <property type="entry name" value="clpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51902">
        <property type="entry name" value="CLPX_ZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ClpX-type ZB" evidence="8">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="112"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="407" mass="45442" checksum="14638CDE74ECEA02" modified="2009-02-10" version="1">MAQKFCSFCGRSSDKVEKLIAGPGNVYICNDCVDIFHDILSEEKRSFSPKKDKKNLPKPSQIKAELDKYVIGQERVKKILSVAVYNHYKRVFSDIDFDDVEIEKSNVILIGPTGSGKTLLARTLAKILDVPFAIADATPLTEAGYVGEDVENVILRLLEVTNFDIERAQYGIIYIDEIDKIARKSPNPSITRDVSGEGVQQALLKIVEGTIANVPPQGGRKHPYQEFLKVDTSNILFIVGGAFDGIEEIIKRRIQSSALGFNAPVKSKKDMRLGEILSHVTPEDLVQYGLIPEFVGRFPVIGALEDLTEDDMVRILTEPKNAILKQYKKLFLLDNVELEVTEEALRAISRKALERGTGARALKSVFEEVMIDAMFELPDLNNIEKVIVTEECVTNKEKPQLVMKESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGB7</accession>
    <name>B7IGB7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_654</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104507.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_654"/>
    <dbReference type="EnsemblBacteria" id="ACJ75131">
        <property type="protein sequence ID" value="ACJ75131"/>
        <property type="gene ID" value="THA_654"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_654"/>
    <dbReference type="eggNOG" id="COG3694">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071040_0_1_0"/>
    <dbReference type="OMA" id="IDFALRP"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010390">
        <property type="entry name" value="DUF990"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06182">
        <property type="entry name" value="ABC2_membrane_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="268" mass="31384" checksum="3B41A1A010FC5709" modified="2009-02-10" version="1">MRTFRKYLEIIKIGVFLSSRRLLYFRVSTFFDILGNTLFLIINIVFWKIIYTNIQFFNGWTIEQMYLFTAFTEFFHVFTKIFFPVSGKLWKLIYTGKLDSYLIKPVEPFMLLNTLNLRLENIFNLIPSVLWILVLINYYKIKLDVLSFIIALLFNFVAVFVYSLIQMSTSTASFWFGKISAIDELSDSLAMLVNYPHTIFPFFIRIFMITLLPFGYASTVPAFVTLKLKYIGTFSALAIGAAFTIILWVTIFKILWKFGIKKYNSYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGE7</accession>
    <name>B7IGE7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Maltose transport system permease protein MalG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_689</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579729.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_689"/>
    <dbReference type="EnsemblBacteria" id="ACJ75161">
        <property type="protein sequence ID" value="ACJ75161"/>
        <property type="gene ID" value="THA_689"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_689"/>
    <dbReference type="eggNOG" id="COG3833">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4907">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_340630_0_0_0"/>
    <dbReference type="OMA" id="QTFYKIV"/>
    <dbReference type="OrthoDB" id="1510342at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="590"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="626"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="656"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="709"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="767"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="591"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="193"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="803" mass="92440" checksum="21E26C2EA732D07E" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGG1</accession>
    <name>B7IGG1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_704</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579740.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_704"/>
    <dbReference type="EnsemblBacteria" id="ACJ75175">
        <property type="protein sequence ID" value="ACJ75175"/>
        <property type="gene ID" value="THA_704"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_704"/>
    <dbReference type="eggNOG" id="COG3631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1683937_0_0_0"/>
    <dbReference type="OMA" id="DFVGPTF"/>
    <dbReference type="OrthoDB" id="1644255at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="2">
        <location>
            <begin position="25"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="147" mass="17805" checksum="18F7611C641E2597" modified="2009-02-10" version="1">MEEYRIRDLEHLKQLWEKTYNTDGKPDWSHLLPYYDDNIHFRDSIQEIYGLKDFSEMVKRLTKRSKKLEMKIVNAVQNGNIMFLEWEMTILFKKTKTSVVYGASRVKLNDTGKIIDQRDYYDLWGDIFDNIPFLGKLYRKFMRKVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGG5</accession>
    <name>B7IGG5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oxidase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_708</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104595.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_708"/>
    <dbReference type="EnsemblBacteria" id="ACJ75179">
        <property type="protein sequence ID" value="ACJ75179"/>
        <property type="gene ID" value="THA_708"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_708"/>
    <dbReference type="eggNOG" id="COG4638">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039484_1_1_0"/>
    <dbReference type="OMA" id="ENLMDMY"/>
    <dbReference type="OrthoDB" id="691776at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044043">
        <property type="entry name" value="VanA_C_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19112">
        <property type="entry name" value="VanA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="5">
        <location>
            <begin position="6"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="326" mass="38469" checksum="1355DFBE8C93DE05" modified="2009-02-10" version="1">MIKNQWYIVMSSKELKKGKLVGVTRFGEKLVFWRSKDGRVNCISDICCHRGASLSHGRLLENDRIMCPFHGFEYDTDGKVKVIPAFGRNVEVPERYKVKKYYTYEIADFIWVWYGDGEPTHIPKYFDDISEEFSYSEFTQVWNVNYTRAIENQLDVVHVPIVHYNTIGKGNRTVVDGPIVKWQNETMFYFYVHNRVDDGTPAKKPDEIDINDISKVYLEFKFPNFWQNHILDKLRVTAAFVPIDEEHTKIYLRFYVAFTKIRFINKIISSIGNIFNRIVLNQDRRVVETQIPKKTELKMNEKLILGDLPIIEYRKKREELKLGNGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGH4</accession>
    <name>B7IGH4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_718</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579751.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_718"/>
    <dbReference type="EnsemblBacteria" id="ACJ75188">
        <property type="protein sequence ID" value="ACJ75188"/>
        <property type="gene ID" value="THA_718"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_718"/>
    <dbReference type="eggNOG" id="COG3857">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_279483_0_0_0"/>
    <dbReference type="OMA" id="YVDCPFK"/>
    <dbReference type="OrthoDB" id="1283891at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="2">
        <location>
            <begin position="248"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="PDDEXK_1" evidence="1">
        <location>
            <begin position="677"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="1006" mass="118955" checksum="928D43DF3ECF8E5C" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGI6</accession>
    <name>B7IGI6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MatE family member</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579760.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_733"/>
    <dbReference type="EnsemblBacteria" id="ACJ75200">
        <property type="protein sequence ID" value="ACJ75200"/>
        <property type="gene ID" value="THA_733"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_733"/>
    <dbReference type="eggNOG" id="COG0534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_0_1_0"/>
    <dbReference type="OMA" id="VGNWIGS"/>
    <dbReference type="OrthoDB" id="1415498at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="421"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="452" mass="49007" checksum="8B54E1C19D9FBEFD" modified="2009-02-10" version="1">METKGVKLLQGDPKRAIVKLSIPMIFAMLVQTMYNLVDGIWVAGLGPAALAAIGAFFPIFMIIISLASGLGVGASSVIARKIGEKNKNEADKAAVNSLAIAVIFGLTFMIVSLALIKPVLEVLGTSKEVLDETLKYAYIVIFSIPLLMFNNVSNGILRGEGDAKKAMYAISIGSILNIFLDPLFIYTFKLGIKGAAYATVFSILVSAILIVFWLFIKKDTYVSIHIKGMKLEAKIINDILRVGIPSSIAQIAMSVAMFVLNVFAVKAGKDLGIAVFTSAWRIINFGTVPLIGIATAVTSVTGAAFGQRDAKKLKTAYLYAIRFGEIISLVVMSSILIFANYIAKAFTYSENGAQIYSELVNALRILSLFLPGVPFGMFTSSMFQGTGYGIRSMIVSINRTIIMQVLFSYLYVFVFNIGLNGVWWGIVTGNAISAAITFTWGIFTVKNIKRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGJ6</accession>
    <name>B7IGJ6_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PS_pyruv_trans domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579769.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_744"/>
    <dbReference type="EnsemblBacteria" id="ACJ75210">
        <property type="protein sequence ID" value="ACJ75210"/>
        <property type="gene ID" value="THA_744"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_744"/>
    <dbReference type="eggNOG" id="COG2327">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_803946_0_0_0"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007345">
        <property type="entry name" value="Polysacch_pyruvyl_Trfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04230">
        <property type="entry name" value="PS_pyruv_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PS_pyruv_trans" evidence="1">
        <location>
            <begin position="13"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="340" mass="39239" checksum="ED7C2C80A48F35C6" modified="2009-02-10" version="1">MKTLLVAIVGNDNFGDEAMFKAIYRKLLSRGEEIVVATYNVDEAKRRFPNVNFIKIPILSKHQYLLGLMGKNVLSIDISRFDSLYISGGGTLNSLYPLHVFNIYLMVREFRKKRKYVEFRPQSVGPFFGRTKPILEFLIKRIVKFSDKFYVREQASYEYLAKKNLKIELHRDDAWEIPADSSISLPDGKYVGLCIRPWKRQEDLLREYMKELVKEIEKLNYIPLFIPIAYGGSESYIDNAFLKGIVPGIFLDDLIEMDSLTPETIKDIIGRCCFTIGMSYHFNVFSLSQGVPSAAIYLDEYYKIKNLGLYKAFGNPKLVFKIPETPPSELLRTILEISNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGK9</accession>
    <name>B7IGK9_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable nicotinate-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="6">2.7.7.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Deamido-NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Deamido-NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nicotinate mononucleotide adenylyltransferase</fullName>
            <shortName evidence="6">NaMN adenylyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">nadD</name>
        <name evidence="8" type="ordered locus">THA_758</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6">Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + H(+) + nicotinate beta-D-ribonucleotide = deamido-NAD(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22860"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="EC" id="2.7.7.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 6">Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NadD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.18" evidence="6"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579781.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_758"/>
    <dbReference type="EnsemblBacteria" id="ACJ75223">
        <property type="protein sequence ID" value="ACJ75223"/>
        <property type="gene ID" value="THA_758"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_758"/>
    <dbReference type="eggNOG" id="COG1057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069765_3_2_0"/>
    <dbReference type="OMA" id="IHIGHLI"/>
    <dbReference type="OrthoDB" id="1433958at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00332"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02165">
        <property type="entry name" value="NMNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00244">
        <property type="entry name" value="NaMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00482">
        <property type="entry name" value="TIGR00482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="7">
        <location>
            <begin position="20"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="208" mass="24293" checksum="E0B12A4D7362450C" modified="2009-02-10" version="1">MAIQFGLPKELLNTKNSLVIFGGSFNPPHNGHIIIAQLVREMFKYADMHIVTSSTPPHKHVDVDFKTRFYLTKKAFEKVKGVEISDIENKLGGISYAINTIEYYEKYYESIFFLVGEDALFSIEKWYRYEDILKKVKMLVYPRYKDKTLYERANNVLKELSNSIYILDLPLIQISSTIVRERVKNKQSIYGFVPESIIELVEEVYGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGM4</accession>
    <name>B7IGM4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Beta-galactosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_775</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579790.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="SMR" id="B7IGM4"/>
    <dbReference type="STRING" id="484019.THA_775"/>
    <dbReference type="CAZy" id="GH1">
        <property type="family name" value="Glycoside Hydrolase Family 1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75238">
        <property type="protein sequence ID" value="ACJ75238"/>
        <property type="gene ID" value="THA_775"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_775"/>
    <dbReference type="eggNOG" id="COG2723">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001859_1_3_0"/>
    <dbReference type="OMA" id="DWVYVVP"/>
    <dbReference type="OrthoDB" id="654705at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="Glyco_hydro_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="PTHR10353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="465" mass="54145" checksum="73D67AEF09B56A68" modified="2009-02-10" version="1">MFSKDFLFGASLSGFQFEMGNPNNEEELDKNTDWFVWVRDLGNIINGKVSGDLPEYGAGYYTNYKAVHNLAKEFGMNALRIGIEWSRIFKESTKDISLDDPNMLEKLDQLADKKAIEHYRDVLEDIKSKGLVAIVNLSHFTLPLWLHDPINVHKGKETEKLGWVSDDAPIEFAKYAEYIAWKFKDIVDMWSSMNEPHVVSQLGYFQTSAGFPPSYFNPSWYLKSLENQALAHNLAYDAIKKHTGKPVGVIYSFTWYDTVNNDEEIFESAMFLNNWNYMDRVKDKIDFVGVNYYTRAVIDRLLVPIKIDNYELNWYTLSGYGYSCVEDGFANSKRPSSEIGWEIYPEGLYNILKEIYNRYGKQIYITENGIADSSDKYRSFYIISHLYAVEKAINEGVPVKGYLHWSIIDNYEWAKGYGKRFGLAYTDFERKTYIPRPSMYILREIIKERSIDKFKGYDPYGLMNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGQ0</accession>
    <name>B7IGQ0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Flagellar M-ring protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">fliF</name>
        <name evidence="15" type="ordered locus">THA_802</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The M ring may be actively involved in energy transduction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the FliF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579806.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_802"/>
    <dbReference type="EnsemblBacteria" id="ACJ75264">
        <property type="protein sequence ID" value="ACJ75264"/>
        <property type="gene ID" value="THA_802"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_802"/>
    <dbReference type="eggNOG" id="COG1766">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028108_2_0_0"/>
    <dbReference type="OMA" id="NYEVNRI"/>
    <dbReference type="OrthoDB" id="167675at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009431">
        <property type="term" value="C:bacterial-type flagellum basal body, MS ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013556">
        <property type="entry name" value="Flag_M-ring_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000067">
        <property type="entry name" value="FlgMring_FliF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006182">
        <property type="entry name" value="FliF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043427">
        <property type="entry name" value="YscJ/FliF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30046">
        <property type="entry name" value="PTHR30046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30046:SF0">
        <property type="entry name" value="PTHR30046:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01514">
        <property type="entry name" value="YscJ_FliF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08345">
        <property type="entry name" value="YscJ_FliF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004862">
        <property type="entry name" value="FliF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01009">
        <property type="entry name" value="FLGMRINGFLIF"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00206">
        <property type="entry name" value="fliF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="15" id="KW-0969">Cilium</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="YscJ_FliF" evidence="13">
        <location>
            <begin position="47"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="YscJ_FliF_C" evidence="14">
        <location>
            <begin position="256"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="288"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="467"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR004862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ACJ75264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="534" mass="59849" checksum="C8517BEB6D9F86E3" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGQ1</accession>
    <name>B7IGQ1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transglycosylase SLT domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_803</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579807.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_803"/>
    <dbReference type="CAZy" id="GH23">
        <property type="family name" value="Glycoside Hydrolase Family 23"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75265">
        <property type="protein sequence ID" value="ACJ75265"/>
        <property type="gene ID" value="THA_803"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_803"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_876980_0_0_0"/>
    <dbReference type="OMA" id="GFNIHAL"/>
    <dbReference type="OrthoDB" id="992177at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLT" evidence="1">
        <location>
            <begin position="68"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="324" mass="37497" checksum="A84F9DAD5B17B725" modified="2009-02-10" version="1">MKKIFFLVILFISTITLSFLFDPLNYITKSNVGLQLQFYNKGGKISIYNPFVYKIVSFDNFTETMRFPEIVKGLAYIESGFNIHALSNVGAMGVAQFKADAAIDYGVFNAWNPQQALLGADRMIRYYYQKYNDIYKVLAIYNIGETNYKKGKYIEQGNAYANKVISASRTVSRKSYLYDRYVLYLQGTLTENSSQTYTAFSFGGIFDYLGIVYFDISSTFHFREGYFPIELGTKSYIRINHFASFVIGYNLGYNLNYENEISIGPIIGYSHFRPIGESYEIYYDFSKGFSLNNFFISIGYGNKYWRINFGFEPEFNNLFINITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGR1</accession>
    <name>B7IGR1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_813"/>
    <dbReference type="EnsemblBacteria" id="ACJ75275">
        <property type="protein sequence ID" value="ACJ75275"/>
        <property type="gene ID" value="THA_813"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_813"/>
    <dbReference type="HOGENOM" id="CLU_3259068_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="42" mass="4785" checksum="A882D5A5F5D8B28F" modified="2009-02-10" version="1">MFESLIFKKLPSILLISGIILVIIGLLIFEKFEERGVNHGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGT4</accession>
    <name>B7IGT4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Maltodextrin glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100452.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_836"/>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75298">
        <property type="protein sequence ID" value="ACJ75298"/>
        <property type="gene ID" value="THA_836"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_836"/>
    <dbReference type="eggNOG" id="COG0366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_399419_0_0_0"/>
    <dbReference type="OMA" id="IDMGHAL"/>
    <dbReference type="OrthoDB" id="1573900at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aamy" evidence="1">
        <location>
            <begin position="109"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="666" mass="78073" checksum="00547677AE748C94" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGU2</accession>
    <name>B7IGU2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Maltose transport system permease protein MalF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_845</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100474.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_845"/>
    <dbReference type="EnsemblBacteria" id="ACJ75306">
        <property type="protein sequence ID" value="ACJ75306"/>
        <property type="gene ID" value="THA_845"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_845"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_3_0"/>
    <dbReference type="OMA" id="FMIVVTQ"/>
    <dbReference type="OrthoDB" id="1619224at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="68"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="293" mass="33552" checksum="AFB25D961B6654EE" modified="2009-02-10" version="1">MQRKEPLIAFWMILPAILVISVIAFFPLFKTFYDSFYSFSLNPRFPRQFVGFGNYIRLFNDYRFWDALKTTIIFTLVSVALETILGLMIALIVNQDFKFRGAVRAAMLIPWAIPTAISSQMWRWMFNDQFGIMSRFYEALHIIEPGTPILGRPNLALWAIIQVDVWKTTPFMALLILAGLQLIPEQLYEAAKIDGANMIQRFFKITLPQLAPTIAVALIFRTLDALRVFDVVYVMTRGSVGTETLSVYNRVLLMDRAFTSASFGYGSSLSVIIFLLISIFAIIYIKSLKLQFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGZ0</accession>
    <name>B7IGZ0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sigma-70 region 2 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100752.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_895"/>
    <dbReference type="EnsemblBacteria" id="ACJ75354">
        <property type="protein sequence ID" value="ACJ75354"/>
        <property type="gene ID" value="THA_895"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_895"/>
    <dbReference type="eggNOG" id="ENOG5033KBK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2157182_0_0_0"/>
    <dbReference type="OMA" id="KCEAFRE"/>
    <dbReference type="OrthoDB" id="1767318at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="1">
        <location>
            <begin position="3"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="109" mass="13194" checksum="56C31C9096076546" modified="2009-02-10" version="1">MDIYKTYKDDVKNIAGRYYKAYKYKILSFEDLEQTIWYILMCALSKFDGRGDQRNFVLHFIKQKLISILKNNRRPPYCTDSPFTCLCFDYFSADDENSKYYKEIHGEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGZ8</accession>
    <name>B7IGZ8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved protein, with A weak D-galactarate dehydratase/altronate hydrolase domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_903</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579872.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_903"/>
    <dbReference type="EnsemblBacteria" id="ACJ75362">
        <property type="protein sequence ID" value="ACJ75362"/>
        <property type="gene ID" value="THA_903"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_903"/>
    <dbReference type="eggNOG" id="COG1672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021114_0_0_0"/>
    <dbReference type="OMA" id="MSMYFLD"/>
    <dbReference type="OrthoDB" id="191489at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018631">
        <property type="entry name" value="AAA-ATPase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012547">
        <property type="entry name" value="PDDEXK_9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09820">
        <property type="entry name" value="AAA-ATPase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08011">
        <property type="entry name" value="PDDEXK_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA-ATPase_like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="526" mass="61543" checksum="895873198093A8F6" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH03</accession>
    <name>B7IH03_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellar P-ring protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Basal body P-ring protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">flgI</name>
        <name evidence="4" type="ordered locus">THA_908</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">Assembles around the rod to form the L-ring and probably protects the motor/basal body from shearing forces during rotation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Periplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FlgI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100778.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_908"/>
    <dbReference type="EnsemblBacteria" id="ACJ75367">
        <property type="protein sequence ID" value="ACJ75367"/>
        <property type="gene ID" value="THA_908"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_908"/>
    <dbReference type="eggNOG" id="COG1706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045235_1_0_0"/>
    <dbReference type="OMA" id="KTIQITR"/>
    <dbReference type="OrthoDB" id="693640at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009428">
        <property type="term" value="C:bacterial-type flagellum basal body, distal rod, P ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00416">
        <property type="entry name" value="FlgI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001782">
        <property type="entry name" value="Flag_FlgI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30381">
        <property type="entry name" value="PTHR30381"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02119">
        <property type="entry name" value="FlgI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01010">
        <property type="entry name" value="FLGPRINGFLGI"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0574">Periplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="329" mass="34840" checksum="EC03E87A35E52B85" modified="2009-02-10" version="1">MKKKIIIALLIVAVFSLSAVVRIKDIAKFRGARDNQLFGIGIVVGLNGTGDGGTLNSPLLANMFKNFGIPISEEDIKSNNTALVMVVADIPPFYKEGMRLDVIVASLGSAKSLENGVLLQTPLYGADGNIYAVAQGPVSVGGAEVKSSVNLQSRFKVTGYIPNGALIEKEIPANIVSDNSVTILLQNPDITTSARVATAINEKFSVKLAKAVDPASVRVQIPDAFSDDIITFLSIIEELEVIPDQAAKVVINERTGTVIFGGDIKISDFTLSYGNFVVSIKNGEIDGQEATISNLISALKALGATPQDIIGIIEELHKAGAIYAEIKVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH12</accession>
    <name>B7IH12_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC transporter, ATP-binding protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_917</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579876.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_917"/>
    <dbReference type="EnsemblBacteria" id="ACJ75376">
        <property type="protein sequence ID" value="ACJ75376"/>
        <property type="gene ID" value="THA_917"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_917"/>
    <dbReference type="eggNOG" id="COG1120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_0"/>
    <dbReference type="OMA" id="DCILDHL"/>
    <dbReference type="OrthoDB" id="1761696at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="2">
        <location>
            <begin position="7"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="239" mass="27509" checksum="1F69BA2911940187" modified="2009-02-10" version="1">MKNTKKVNLEAVDFSGFSNNVPLFENITFSLKEGEILVLYGPRGSSKSALLRSFSRLNEEVYDDIKYTGSAFVCQQDLFSYSIKEIRNLVLYVDTNFFEALDYLSFGEFLELATGNEMLSFDDYIPLLDDFGVLKALTDKFDTKLSTFYVLEKISALLFVAYLKKSKVIILDCILDHLDDEHLNKISKILRKNVVSNEKSLIIATRFFKRFLPLADLFISMKNGKIEFRGEPREYTIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH20</accession>
    <name>B7IH20_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MnhC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_925</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100808.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_925"/>
    <dbReference type="EnsemblBacteria" id="ACJ75384">
        <property type="protein sequence ID" value="ACJ75384"/>
        <property type="gene ID" value="THA_925"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_925"/>
    <dbReference type="eggNOG" id="COG1006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082058_2_2_0"/>
    <dbReference type="OMA" id="FFGIILK"/>
    <dbReference type="OrthoDB" id="2078994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="108" mass="12044" checksum="BC27A0B838E8A0AD" modified="2009-02-10" version="1">MVDFIPPFVIILLSIYGLLFKRHIISKVIALDVLNTGVVYLFVVIASKAGEYYPIKTEGVKNYADPFPQAVIITSIVIGFATLSLLLMISMLVVEKKKKTDIVKIEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH41</accession>
    <name>B7IH41_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exonuclease sbcc, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_946</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579893.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_946"/>
    <dbReference type="EnsemblBacteria" id="ACJ75405">
        <property type="protein sequence ID" value="ACJ75405"/>
        <property type="gene ID" value="THA_946"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_946"/>
    <dbReference type="eggNOG" id="COG0419">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004785_0_2_0"/>
    <dbReference type="OMA" id="ISHVQEM"/>
    <dbReference type="OrthoDB" id="1143316at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_23" evidence="2">
        <location>
            <begin position="8"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="175"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="487"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="641"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="927" mass="109625" checksum="B53ED3B647CC7D21" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH66</accession>
    <name>B7IH66_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_971</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100937.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_971"/>
    <dbReference type="EnsemblBacteria" id="ACJ75430">
        <property type="protein sequence ID" value="ACJ75430"/>
        <property type="gene ID" value="THA_971"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_971"/>
    <dbReference type="eggNOG" id="COG0513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_21_1_0"/>
    <dbReference type="OMA" id="FGCQALV"/>
    <dbReference type="OrthoDB" id="626183at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005580">
        <property type="entry name" value="DbpA/CsdA_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03880">
        <property type="entry name" value="DbpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Q_MOTIF" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="33"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="212"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="516" mass="58970" checksum="66EA5ECAB149D459" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH96</accession>
    <name>B7IH96_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579921.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1001"/>
    <dbReference type="EnsemblBacteria" id="ACJ75460">
        <property type="protein sequence ID" value="ACJ75460"/>
        <property type="gene ID" value="THA_1001"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1001"/>
    <dbReference type="HOGENOM" id="CLU_2332707_0_0_0"/>
    <dbReference type="OMA" id="ADNQTHI"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="101" mass="11788" checksum="F668B5D240D2E523" modified="2009-02-10" version="1">MNEEKYKEIISIVKIETMYKPGDLLEDRIIKSIKRKRLMSRLKKSSLVLIIVGAAVTVSFFTFDIKRPQNQLYTYEQQQSQDYVVKDFEPILNISLVSDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHB9</accession>
    <name>B7IHB9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Bacterioferritin comigratory protein, AhpC/TSA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1025</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101052.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1025"/>
    <dbReference type="EnsemblBacteria" id="ACJ75483">
        <property type="protein sequence ID" value="ACJ75483"/>
        <property type="gene ID" value="THA_1025"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1025"/>
    <dbReference type="eggNOG" id="COG1225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_2_0"/>
    <dbReference type="OMA" id="VCTKELC"/>
    <dbReference type="OrthoDB" id="892697at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="2"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="3">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="154" mass="17385" checksum="06E9F34495BCE4A7" modified="2009-02-10" version="1">MLEKGSMYIDFELVNTKLEKVKLSSLIGQKIVLVFYPGAFTSVCEKELCSFRDMMAKFNNLNAKVFGISVDSPFANKAFAEKNRLNFELLSDFGGIVSRKYGGVHENFVGIENYTVSKRAVYVIDEEGKIIYSWVSDDPGKEPPYNEVEEVIAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHG0</accession>
    <name>CBID_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cobalt-precorrin-5B C(1)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.195</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cobalt-precorrin-6A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiD</name>
        <name type="ordered locus">THA_1068</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylation of C-1 in cobalt-precorrin-5B to form cobalt-precorrin-6A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Co-precorrin-5B + S-adenosyl-L-methionine = Co-precorrin-6A + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:26285"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:60063"/>
            <dbReference type="ChEBI" id="CHEBI:60064"/>
            <dbReference type="EC" id="2.1.1.195"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 6/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbiD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.195" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579975.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHG0"/>
    <dbReference type="SMR" id="B7IHG0"/>
    <dbReference type="STRING" id="484019.THA_1068"/>
    <dbReference type="EnsemblBacteria" id="ACJ75524">
        <property type="protein sequence ID" value="ACJ75524"/>
        <property type="gene ID" value="THA_1068"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1068"/>
    <dbReference type="eggNOG" id="COG1903">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041273_1_0_0"/>
    <dbReference type="OMA" id="YHGKLIK"/>
    <dbReference type="OrthoDB" id="1282567at2"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00227"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043780">
        <property type="term" value="F:cobalt-precorrin-5B C1-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046140">
        <property type="term" value="P:corrin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00787">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002748">
        <property type="entry name" value="CbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036074">
        <property type="entry name" value="CbiD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863">
        <property type="entry name" value="PTHR35863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01888">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026782">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111342">
        <property type="entry name" value="SSF111342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00312">
        <property type="entry name" value="cbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000133749" description="Cobalt-precorrin-5B C(1)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00787"/>
        </source>
    </evidence>
    <sequence length="351" mass="38768" checksum="B28EDAD791A6E440" modified="2009-02-10" version="1">MKKELRYGYTTGSCATAAAKAATYMLFNDEILKSIKIDLPIGKEIELDIFYIERKEGEVICGVRKDAGDDPDVTHNMIIYAKAEKSKEFLITGGEGIGVVTKKGLPLQVGDYAINPVPRKMIESEVKKVLPEGKNVKITIFAPEGKYIAKRTLNPKLGIIGGISILGTTGIVEPLSDEAYKKTIDLEISMASSESNEICLVFGNYGKNFTTLSSKMPLVTMGNYVGFALESACKHRIKKVYLVGQIGKMIKVAGGIFNTYSYIADARNEIFTAYLSLYGLDRGILEKVMSANTTEEILDLIEGKVGKDFFENLALRIKEKCTQYVKGCLEVEVEIFSLKKGHLAKTWSDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHJ7</accession>
    <name>B7IHJ7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, crp family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1108</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580003.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1108"/>
    <dbReference type="EnsemblBacteria" id="ACJ75561">
        <property type="protein sequence ID" value="ACJ75561"/>
        <property type="gene ID" value="THA_1108"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1108"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075053_4_1_0"/>
    <dbReference type="OMA" id="GEYICYE"/>
    <dbReference type="OrthoDB" id="1596937at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13545">
        <property type="entry name" value="HTH_Crp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51063">
        <property type="entry name" value="HTH_CRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="13"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="HTH crp-type" evidence="3">
        <location>
            <begin position="148"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="229" mass="26546" checksum="22DB9656C091E5B6" modified="2009-02-10" version="1">MKQYIESIKNCSLFKDLNENEILEITKKSKILTFEKDEIIKQRNDPIDEILILIEGEALGLFVNEEGRVIQIDHMIAPKTLAIATIFSSNPKYPVDVVAIKKCKILTLSKDILIHEMMKNEKILKNYLQNVSDTFIFITDRFYEITLKNLIQKVCSFLYDSFKKQNSKTIKLEISKTELAREFGVTRPALSRVFIELEKKGIIEMEGKNVKIKDLRYIEVYCSPEGLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHM7</accession>
    <name>B7IHM7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580027.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1139"/>
    <dbReference type="EnsemblBacteria" id="ACJ75591">
        <property type="protein sequence ID" value="ACJ75591"/>
        <property type="gene ID" value="THA_1139"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1139"/>
    <dbReference type="HOGENOM" id="CLU_739505_0_0_0"/>
    <dbReference type="OMA" id="IQITGYF"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="374" mass="42867" checksum="B4D270E0E51916DA" modified="2009-02-10" version="1">MKKLFFFLIIFLFAITSLSFVNYDNNFKLFKLNFSFNPYLYQFIYNVDNLNNLLNDGILDINKINSFRVGIFGNTHASASIKIGKFSLTPFAELDMNLLLSIPDDLFKFLTNDIEIDKTYEYDEIKIPSYSNTFLNIGVALKFNNLSISPYVYTPVIYISEKDQKFYFKYTSSSSPSKINLELNYDLIGYIPINTESENEAKKSPTGIAASLGYSNSRLGIFVNNITIKPITLPSAKLLRITTDATYENLSFEIVDTNLEVKSIDKEIKISPKLNLSGYYNFKFLADFHLEGMYYFDRSYTLSAEIYKKIFFLIPFYKFGYYSKNSVFSNTLGLGIDLILLKSNFGITITSDKIIPSKDSYQGFGVAFNFEFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHN0</accession>
    <name>B7IHN0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="10">L-asparaginase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_1142</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparaginase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580030.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1142"/>
    <dbReference type="EnsemblBacteria" id="ACJ75594">
        <property type="protein sequence ID" value="ACJ75594"/>
        <property type="gene ID" value="THA_1142"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1142"/>
    <dbReference type="eggNOG" id="COG0252">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019134_1_0_0"/>
    <dbReference type="OMA" id="RKNHTSR"/>
    <dbReference type="OrthoDB" id="1659551at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006528">
        <property type="term" value="P:asparagine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08964">
        <property type="entry name" value="L-asparaginase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004550">
        <property type="entry name" value="AsnASE_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="SSF53774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asparaginase" evidence="8">
        <location>
            <begin position="3"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase_C" evidence="9">
        <location>
            <begin position="206"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="88"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="O-isoaspartyl threonine intermediate" evidence="3">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604550-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="335" mass="36865" checksum="F52B29082A74F712" modified="2009-02-10" version="1">MKKVVILTTGGTIAMVKNEQGVVPYDKGSALISEIPSLKNLGVKIEVREFSNIPSPHMTPQKMWELSRTIDEIQKDDDVIGVVVTHGTDTLEETSYLLDLTLKSEKPVVCTAAMRNIGELGTDGPRNVYSSVLTVLSPQANQMGVMVCLNDEIHAAREVTKTYTSNVATFDSPGYGPLGIVDEDNVIFFRKSLTREKILVDKIEERVALIKTFTGDDGKLLKYAAEIGYKGIILEGFGRGNVPPLVAEAVEEVIKEKQIPVIITSRCFKGRVYPVYGYAGGGADLRKKGAIMSEHPIGQKAKIKLMVVLGKTSNLEEIRQYFEPNISRRIILHEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHP2</accession>
    <name>B7IHP2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1154"/>
    <dbReference type="EnsemblBacteria" id="ACJ75606">
        <property type="protein sequence ID" value="ACJ75606"/>
        <property type="gene ID" value="THA_1154"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1154"/>
    <dbReference type="eggNOG" id="ENOG502ZHXP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1947815_0_0_0"/>
    <dbReference type="OMA" id="QVVPQQW"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="110" mass="12413" checksum="7D927050A178BF2A" modified="2009-02-10" version="1">MKRGSLLVEALVTLIIIVFAFGVAFVPSWSLLRKTKENSELISLEELLLNKCEELTYLNVSSIVPQDSYEEYNGVKYKVSISKKNIESSDIFTLTSDSTFITFPEITIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHQ3</accession>
    <name>B7IHQ3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sec-independent protein tatd</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101322.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1165"/>
    <dbReference type="EnsemblBacteria" id="ACJ75617">
        <property type="protein sequence ID" value="ACJ75617"/>
        <property type="gene ID" value="THA_1165"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1165"/>
    <dbReference type="eggNOG" id="COG0084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031506_4_0_0"/>
    <dbReference type="OMA" id="PNEAPRI"/>
    <dbReference type="OrthoDB" id="915852at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004536">
        <property type="term" value="F:deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015991">
        <property type="entry name" value="Hydrolase_TatD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00010">
        <property type="entry name" value="TIGR00010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01090">
        <property type="entry name" value="TATD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="252" mass="28909" checksum="A5845B220C2DBD04" modified="2009-02-10" version="1">MKIVDTHAHLHMKHFNEDREKIIKNFENDGIEFIVNVATNIEDSYLCIDLSKKYERIFTTVGVHPHDSSNVPDDYLGILEKLAKNEKVVAIGEIGLDYYRNFSPKEVQQKVFAEQLMLAKDLNLPVVVHVRDAYEDAYSILEMIGPFNGVIHSFSGDREYALKFVKLGFYLGIGGPLTYKKNQQLRDIVRLVGEENIVTETDCPYLPPQPYRGKRNEPSYVKYVVEEINKLIGENVSEKLINNARELFGVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHT0</accession>
    <name>B7IHT0_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Flagellar biosynthetic protein FliP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">fliP</name>
        <name evidence="13" type="ordered locus">THA_1194</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a role in the flagellum-specific transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the FliP/MopC/SpaP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580073.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1194"/>
    <dbReference type="EnsemblBacteria" id="ACJ75644">
        <property type="protein sequence ID" value="ACJ75644"/>
        <property type="gene ID" value="THA_1194"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1194"/>
    <dbReference type="eggNOG" id="COG1338">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042028_0_1_0"/>
    <dbReference type="OMA" id="MPPNQIL"/>
    <dbReference type="OrthoDB" id="1049349at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005837">
        <property type="entry name" value="FliP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005838">
        <property type="entry name" value="T3SS_IM_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30587">
        <property type="entry name" value="PTHR30587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30587:SF0">
        <property type="entry name" value="PTHR30587:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00813">
        <property type="entry name" value="FliP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00951">
        <property type="entry name" value="FLGBIOSNFLIP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01302">
        <property type="entry name" value="TYPE3IMPPROT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01103">
        <property type="entry name" value="fliP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01061">
        <property type="entry name" value="FLIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="7 12" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="11 12" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="5 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="13" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="46"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ75644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="251" mass="28231" checksum="CE06ED4E77547A9A" modified="2009-02-10" version="1">MKRLFVLIFLVIVFVSFAQDEVPLPNISIQLSPPQQPRDLVNTLEILLLLTVLSLAPSILILFTSFTRIIIVFSFLRNALGTRQTPPNQILIGLALFLTFFIMQPVWNDIYNNAITPYLNSEIGYEELFSRSMDRLRTFMITELKIHHNEDNVFMLADNVGKEIDSVEEAPNSVLIPAFVIGELEIGFKMGILLYIPFIVMDMVVASILLSLGMIMIPPVLVSLPFKILLFVLVNGWDMLIGSLIKSFGGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHT2</accession>
    <name>KTHY_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thymidylate kinase</fullName>
            <ecNumber evidence="1">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tmk</name>
        <name type="ordered locus">THA_1196</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + dTMP = ADP + dTDP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580074.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHT2"/>
    <dbReference type="SMR" id="B7IHT2"/>
    <dbReference type="STRING" id="484019.THA_1196"/>
    <dbReference type="EnsemblBacteria" id="ACJ75646">
        <property type="protein sequence ID" value="ACJ75646"/>
        <property type="gene ID" value="THA_1196"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1196"/>
    <dbReference type="eggNOG" id="COG0125">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049131_0_2_0"/>
    <dbReference type="OMA" id="VMTREPG"/>
    <dbReference type="OrthoDB" id="1585072at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:thymidylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000190769" description="Thymidylate kinase">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <sequence length="201" mass="22840" checksum="09CCE7954247BEC3" modified="2009-02-10" version="1">MFIAFEGIDGSGKSTQLNLLSQYLKSKGKKVLNIREPGGTILGEKIREILLDNNLNINKRSELLLFLASRAQLVEEVIKPHLKRGFFVLADRFSDSSIAYQGGARNIGVETVSTLNDFATDNIYPDIVFYIDIPTKIAMERLKDKNLDRLEKEGQIFLEKVRNTYLKLSKLRDNFFIIDGTKSIDNVFAEIKSIVEKHLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMW7</accession>
    <name>C0QMW7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_2041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580555.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2041"/>
    <dbReference type="EnsemblBacteria" id="ACN29534">
        <property type="protein sequence ID" value="ACN29534"/>
        <property type="gene ID" value="THA_2041"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2041"/>
    <dbReference type="eggNOG" id="COG3547">
        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="HOGENOM" id="CLU_036902_4_1_0"/>
    <dbReference type="OMA" id="KQSIHHI"/>
    <dbReference type="OrthoDB" id="655500at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transposase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEDD_Tnp_IS110" evidence="2">
        <location>
            <begin position="20"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="258"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACN29534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="419" mass="48900" checksum="277FBC1AA45ACCD0" modified="2009-05-05" version="1">MKQKSINKKISRITQDTLIVGIDVAKRTHWIRMMDFRGIDLIKPFKINNTMDGIKMLEEKIKSVKEENKLNKVVLGMEPSGHYWKVLAWQMKISKEIDYLVGVNPYHVKKSKEFDDNSPGKSDRKDAGVIARLIRDGRYFDIYLPEDIYGELRVITTTREQLVNKRKNAKNMVISVMDEYFPEYEKIYKNIFSEGSIKLLKTHPFPKEIIEVGIEKIEKVLKESTNGKDWRNRAQKIYEAAKKSIGVREGQKSAKIKLRMLLEEIEFLTSQIEELEKEMEKLVEETEEGEYIKSVPGIGAIMTAIILGEVGDISRFKSWKEIRKLAGLNLYEISSGEHKGKTRITKRGRPLLRKIIYLMAQTTLKHNHEIRKKYEQLRRREKNPLKVVQALIAIGLKMIRIVFKLAKSKIKYEPEKVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICK0</accession>
    <name>B7ICK0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phospholipase/Carboxylesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580116.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="SMR" id="B7ICK0"/>
    <dbReference type="STRING" id="484019.THA_1282"/>
    <dbReference type="EnsemblBacteria" id="ACJ75727">
        <property type="protein sequence ID" value="ACJ75727"/>
        <property type="gene ID" value="THA_1282"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1282"/>
    <dbReference type="eggNOG" id="COG4099">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1105878_0_0_0"/>
    <dbReference type="OMA" id="ANHNSWD"/>
    <dbReference type="OrthoDB" id="1195966at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_2" evidence="1">
        <location>
            <begin position="31"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="241" mass="27537" checksum="BFA106AAFA3BDC31" modified="2009-02-10" version="1">MNYLDLFKPFVYKGEFITLPYRLFSPKIENNKRYPLIVFLHGAGERGNDNIKQITANKGATVWASFDVQSENPCFILAPQCPENSWWGSYKKDEFVFEANTLLYTVMLLINKISKENPIDENRIYITGLSMGGFGTLILLSDFPEKFAAGVVVCGGGDLDKVSRYKDVPIWLFHAEDDPVVSVDFSRKLFEKSKSIGGNIKYTEYPKGLLSSQKIHPHAAWELAYNDKAMIKWLFNQVKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICM6</accession>
    <name>B7ICM6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Efflux ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1308</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580139.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1308"/>
    <dbReference type="EnsemblBacteria" id="ACJ75753">
        <property type="protein sequence ID" value="ACJ75753"/>
        <property type="gene ID" value="THA_1308"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1308"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4591">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_334317_0_0_0"/>
    <dbReference type="OMA" id="LNIMYGL"/>
    <dbReference type="OrthoDB" id="43185at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="506"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="729"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="770"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="816"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="16"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="278"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="754"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="854" mass="96546" checksum="5F71FA3DD5D3F31A" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICN1</accession>
    <name>B7ICN1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Riboflavin transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_1313</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Probably a riboflavin-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101634.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1313"/>
    <dbReference type="EnsemblBacteria" id="ACJ75758">
        <property type="protein sequence ID" value="ACJ75758"/>
        <property type="gene ID" value="THA_1313"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1313"/>
    <dbReference type="eggNOG" id="COG3601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086673_0_1_0"/>
    <dbReference type="OMA" id="MFLEFPI"/>
    <dbReference type="OrthoDB" id="1497256at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032217">
        <property type="term" value="F:riboflavin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024529">
        <property type="entry name" value="ECF_trnsprt_substrate-spec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025720">
        <property type="entry name" value="RibU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38438">
        <property type="entry name" value="PTHR38438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12822">
        <property type="entry name" value="ECF_trnsprt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037778">
        <property type="entry name" value="UCP037778_transp_RibU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="177" mass="19666" checksum="64129244DB49DF6F" modified="2009-02-10" version="1">MLNAKRVAIIGVFAALAFVVMYIEIPIFPAVNFLKYDPSDILVLLTAFIYSPTVGVIVLAIKDILFFIFKSQDIVGIAMNFAAGALFIVPAAIIYGKKNRFREILGYIIGTVAATGGMALLNMIVVPYYWKIPLEEVFKLLPWIIGFNAIKFSIDSVINGIIHRRVKNILEPDEAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICN9</accession>
    <name>B7ICN9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1321</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101652.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1321"/>
    <dbReference type="EnsemblBacteria" id="ACJ75766">
        <property type="protein sequence ID" value="ACJ75766"/>
        <property type="gene ID" value="THA_1321"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1321"/>
    <dbReference type="eggNOG" id="ENOG5033IE2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_189039_0_0_0"/>
    <dbReference type="OMA" id="KACITDE"/>
    <dbReference type="OrthoDB" id="2073822at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="76" mass="8977" checksum="85C7FE50252BC593" modified="2009-02-10" version="1">MNDLEYIAQSCRDDERLLNIVKDVANMSGEEREDFAKKVRQYFLGKNSEEDNNAYKFFKIILEDENARKILEILGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICP4</accession>
    <name>B7ICP4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrolase, ama/HipO/hyuC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1326</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580147.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1326"/>
    <dbReference type="EnsemblBacteria" id="ACJ75771">
        <property type="protein sequence ID" value="ACJ75771"/>
        <property type="gene ID" value="THA_1326"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1326"/>
    <dbReference type="eggNOG" id="COG1473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023257_0_1_0"/>
    <dbReference type="OMA" id="RAHACGH"/>
    <dbReference type="OrthoDB" id="796259at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050118">
        <property type="term" value="F:N-acetyldiaminopimelate deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023905">
        <property type="entry name" value="AcetylDAP_deacetylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PTHR11014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014:SF98">
        <property type="entry name" value="PTHR11014:SF98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="1">
        <location>
            <begin position="172"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="364" mass="41361" checksum="C0A67CD7126EE78A" modified="2009-02-10" version="1">MDSIELRHILHQNPEISFREFETQKILLSALKDLKDERLKIYKIAGTGVIALYEPKKGEPFILYRSDIDGLPIEEETEWEFASKNSNMHACGHDVHMSIAYELIKKILSSNIEKNFVFIFQPGEETGAGARYVLDEIEELDIKLALALHVTDEYDFKTVATTKGVLFASATEIDLTFSGKASHVAFYKSGIDSIYLASKFLTEFYNLTFDKAIARFGKIEGGNARNIVSPQTTLMGSIRSESLKITEEIINKIQEVAKSVVQKDGGEFSISKGSIYPPVIVNDKLYELFREFVQKNNIQFIDCGMKYTGEDFGYFSQEYPSLMFWAGVSKGEKYGLHNPKFLPNDEVILYYADVVFNFLKELEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICQ4</accession>
    <name>B7ICQ4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580155.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1336"/>
    <dbReference type="EnsemblBacteria" id="ACJ75781">
        <property type="protein sequence ID" value="ACJ75781"/>
        <property type="gene ID" value="THA_1336"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1336"/>
    <dbReference type="eggNOG" id="ENOG50344SH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_506138_0_0_0"/>
    <dbReference type="OMA" id="ISITLEW"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="538" mass="60673" checksum="0B404D0ECB128468" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICR0</accession>
    <name>B7ICR0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fructokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1342</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580160.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1342"/>
    <dbReference type="EnsemblBacteria" id="ACJ75787">
        <property type="protein sequence ID" value="ACJ75787"/>
        <property type="gene ID" value="THA_1342"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1342"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_6_1_0"/>
    <dbReference type="OMA" id="DLWKGRV"/>
    <dbReference type="OrthoDB" id="1604782at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="3">
        <location>
            <begin position="2"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="300" mass="33376" checksum="701956151634B327" modified="2009-02-10" version="1">MILFVGELLADMIAEEPLDTAKHFEMKVGGSPGNIASYLSQLGVKARILSRVGNDIIGKRIINKLRQKGVDTSYIQIDKEYGTTLVFVQKTKDTPDFFVLRGADRFINLPNNDIFNGIKILHLSCWTISYNGLFKKIVSILGEAIKNRILIGFDPNCRDKIFKCDKIDIEKVKFILKHTNFCKPSLDDAVAIFGEKDYSLEDTIKYYIDKFHALGVKNIALTAGKFGAYVSDGKNTLHIPSNAKKVVDATGAGDGFWAGMYYGILNGKNFVESCKMGSKVSAHILKFVGADVELSNKIWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICR8</accession>
    <name>B7ICR8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1350</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Bacterial flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580167.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1350"/>
    <dbReference type="EnsemblBacteria" id="ACJ75795">
        <property type="protein sequence ID" value="ACJ75795"/>
        <property type="gene ID" value="THA_1350"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1350"/>
    <dbReference type="eggNOG" id="COG1344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011142_2_0_0"/>
    <dbReference type="OMA" id="FEHTINN"/>
    <dbReference type="OrthoDB" id="636786at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001492">
        <property type="entry name" value="Flagellin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046358">
        <property type="entry name" value="Flagellin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042187">
        <property type="entry name" value="Flagellin_C_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001029">
        <property type="entry name" value="Flagellin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42792">
        <property type="entry name" value="PTHR42792"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00700">
        <property type="entry name" value="Flagellin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00669">
        <property type="entry name" value="Flagellin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00207">
        <property type="entry name" value="FLAGELLIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Flagellin_N" evidence="4">
        <location>
            <begin position="3"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Flagellin_C" evidence="5">
        <location>
            <begin position="303"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="387" mass="41410" checksum="4B3A6778407390CC" modified="2009-02-10" version="1">MRINHNISALNAWRSIGQTNMSMTKTLERLSSGLRINRAGDDAAGLAISEKMRGQIKGLNMAIKNSQDAISLIQTAEGALTEVHSILQRMRELSVQASSDTNTDVDRNQIQAELDQLREEIDRIARTTEFNTKKLLDGKLESFRSEVDAKVVTGGNINLTLGAVNTNASEGTYIVEVGQFNGNETSALDVKITLITAGGTSDTSNTLGAASASLGSIKFKWDSTVFDINDFGGALPKGEVVDSAVVRVEATNTSANQLIFQIGSNEGHNMIAGIDDMSAGALGITTTSLDVTTQDAAERSIMVIDAAIHKVSSQRAALGAVQNRLEHTISNLGVAAENLTAAESRVRDADMAKEMMEFTKQQILLQSSMGMLAQANAQPQNVLQLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICX5</accession>
    <name>B7ICX5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101811.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1408"/>
    <dbReference type="EnsemblBacteria" id="ACJ75852">
        <property type="protein sequence ID" value="ACJ75852"/>
        <property type="gene ID" value="THA_1408"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1408"/>
    <dbReference type="eggNOG" id="COG1811">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091659_0_0_0"/>
    <dbReference type="OMA" id="IYCVGAM"/>
    <dbReference type="OrthoDB" id="1815957at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007563">
        <property type="entry name" value="DUF554"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36111">
        <property type="entry name" value="PTHR36111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04474">
        <property type="entry name" value="DUF554"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="224" mass="23517" checksum="5CC096269AECD416" modified="2009-02-10" version="1">MKNILTPVVNAIAVVIGSLIGMILKKGFPENIRKILFFAVGLSTIGMAINMIIKANNFLIVLGSMAIGGIIGELINIEDKLKKIGDSIKEGDFSTGFVASSILFLVGPLTIVGSITAGLTKDGSLIYMKSLLDGISSIVLSSIYGLGVMLSAISVLLVQGTIVLLSSKLSFLTNPLYLNDLVAVGGVMVLGIGLKILDIKDTKVGNFLPALFISPILSFLTSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICX6</accession>
    <name>B7ICX6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integral membrane protein DUF6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1409</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1409"/>
    <dbReference type="EnsemblBacteria" id="ACJ75853">
        <property type="protein sequence ID" value="ACJ75853"/>
        <property type="gene ID" value="THA_1409"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1409"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084683_0_0_0"/>
    <dbReference type="OMA" id="AYKVVIS"/>
    <dbReference type="OrthoDB" id="1687022at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="3"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="140"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="276" mass="31261" checksum="047142FD8C8783C4" modified="2009-02-10" version="1">MWITIRIILLGFERIFGKILTKNSSPIISAWGFFGFSTLMFLPFINYLSFDIIKVSLISGTIYSFSFFLYMYALSKEDVSVVAPLYNINAIFLIFISFLFLSEKITIQKIVGSILMIYGVSYLKKEKSISSSYKNILKSKGALAMIISSLLMAIGRVVDGFLTTSFNKLGYSLGVYLIITIYFFTVSLLSHKNVKVHIDYTKKNIIYLLLGGFCNAYAYFALLMAFKYMDVSIAEPVSMISALVSVLFSYIFFKEKIKVRIFGTILLISGAFVIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICZ5</accession>
    <name>B7ICZ5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Permeases of the drug/metabolite transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1428</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101847.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1428"/>
    <dbReference type="EnsemblBacteria" id="ACJ75872">
        <property type="protein sequence ID" value="ACJ75872"/>
        <property type="gene ID" value="THA_1428"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1428"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_21_3_0"/>
    <dbReference type="OMA" id="MILEPIW"/>
    <dbReference type="OrthoDB" id="1425840at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="6"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="134"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="280" mass="31397" checksum="62A6534D4C4D52C1" modified="2009-02-10" version="1">MKIVAIVNLLLVTLIWGLTFPIQKMVLVDISPFAYNAIRFWIATVLSFFVFGKGNKYGLILGVVLGISYAAQTWGLTLTTSSKSGFITAFYIVLIPIFSYFIEKEKPTKVQIFSFLIAMIGEYLLSGGIDSINFGDILTFFCAILYALHVVLVTEFSKKSPEKDLLTSQFLMVALLNSLFGLNQSWKIDVKIFSVALFTAIFATIYALIAQAKYQKVVGSNTSALIFVGEPVFATIFSIILLSERLDMLQIFGVVLTMFALILSIIPEKYFRNFFRHVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICZ7</accession>
    <name>B7ICZ7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Flagellar basal-body rod protein FlgC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">flgC</name>
        <name evidence="8" type="ordered locus">THA_1430</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4 5">The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod. The rod consists of about 26 subunits of FlgG in the distal portion, and FlgB, FlgC and FlgF are thought to build up the proximal portion of the rod with about 6 subunits each.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101852.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1430"/>
    <dbReference type="EnsemblBacteria" id="ACJ75874">
        <property type="protein sequence ID" value="ACJ75874"/>
        <property type="gene ID" value="THA_1430"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1430"/>
    <dbReference type="eggNOG" id="COG1558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123272_0_0_0"/>
    <dbReference type="OMA" id="YVAYPNI"/>
    <dbReference type="OrthoDB" id="1870921at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030694">
        <property type="term" value="C:bacterial-type flagellum basal body, rod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019776">
        <property type="entry name" value="Flagellar_basal_body_rod_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006299">
        <property type="entry name" value="FlgC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01395">
        <property type="entry name" value="FlgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00588">
        <property type="entry name" value="FLAGELLA_BB_ROD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="6">
        <location>
            <begin position="9"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="7">
        <location>
            <begin position="87"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="137" mass="15492" checksum="C8D5E65347435339" modified="2009-02-10" version="1">MNEFNIMTISATGMSAQRLRIDVISNNLANSETTRTENGEPYRRKVPVFQELLRNINGRLENGGVVVKKIYEDKSPFRIVYDPTHPDADENGYVKLPNVNVVREMVDMINAQRAYEANVTAFNTTKAMINSALQIGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID01</accession>
    <name>Y1434_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0145 protein THA_1434</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">THA_1434</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0145 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101860.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7ID01"/>
    <dbReference type="SMR" id="B7ID01"/>
    <dbReference type="STRING" id="484019.THA_1434"/>
    <dbReference type="EnsemblBacteria" id="ACJ75878">
        <property type="protein sequence ID" value="ACJ75878"/>
        <property type="gene ID" value="THA_1434"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1434"/>
    <dbReference type="eggNOG" id="COG0393">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117144_1_2_0"/>
    <dbReference type="OMA" id="ETRNQAM"/>
    <dbReference type="OrthoDB" id="2015368at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00338">
        <property type="entry name" value="UPF0145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002765">
        <property type="entry name" value="UPF0145"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035439">
        <property type="entry name" value="UPF0145_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068">
        <property type="entry name" value="PTHR34068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01906">
        <property type="entry name" value="YbjQ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117782">
        <property type="entry name" value="SSF117782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000120020" description="UPF0145 protein THA_1434">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00338"/>
        </source>
    </evidence>
    <sequence length="108" mass="11623" checksum="1AD8F44C1B2DE93C" modified="2009-02-10" version="1">MIITTTENIHGYEIVEILGIVMGNIVQSKHLGKDIAAAFKTLAGGEIKAYTEMMTEARNKAIERMIDEAEKIGADAVVNVRFSSSSVMSGAAEMLAYGTAVKIKKSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID02</accession>
    <name>B7ID02_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1435</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580222.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1435"/>
    <dbReference type="ESTHER" id="theab-b7id02">
        <property type="family name" value="Bacterial_EstLip_FamX"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75879">
        <property type="protein sequence ID" value="ACJ75879"/>
        <property type="gene ID" value="THA_1435"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1435"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033707_0_0_0"/>
    <dbReference type="OMA" id="NWWTQSG"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024981">
        <property type="entry name" value="DUF3887"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13026">
        <property type="entry name" value="DUF3887"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3887" evidence="3">
        <location>
            <begin position="24"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Hydrolase_4" evidence="2">
        <location>
            <begin position="179"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="417" mass="47820" checksum="AFFF30FF87DEC78C" modified="2009-02-10" version="1">MKKVIFALLVVSITIFPIEYQLVAQSYFEGLISKNFEKCYELSSDILKKHLPIGKLKEIWEGIEQTYGPFSNIIKITPIEKSPYMIYIYTAKFQKGALNISITVNNQGKIDGLFFNIAQNLEYKAPNYVKNDNFTEKDIKVGDLPGKLTIPKNPTDVAVILIHGSGPNDMDETIGPNKIFKDIAYGLSSNNIAVLRYDKRTLHPELLKDPENITVKEEVIDDVERAVKLLKKEGYKKIYLLGHSLGAYLAPYIAYKNQDIYGLILLAPPARNIEEITLDQLEFLEKLYSGKNKEEIKKAKEEVIKLTNNKASKNEIILGAPASYWYDLRNYNPLKYIKQLKIPVLMLFGENDYQVTTKEFEIFKKELSEKKNIKIKMYSNLTHIFTPGEKSPYSYYIENHVSSKVIDDIVSFIEGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID08</accession>
    <name>B7ID08_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acetate CoA-transferase subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1442</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101873.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1442"/>
    <dbReference type="EnsemblBacteria" id="ACJ75885">
        <property type="protein sequence ID" value="ACJ75885"/>
        <property type="gene ID" value="THA_1442"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1442"/>
    <dbReference type="eggNOG" id="COG1788">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019942_2_1_0"/>
    <dbReference type="OMA" id="FVKAWKG"/>
    <dbReference type="OrthoDB" id="1660857at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012792">
        <property type="entry name" value="3-oxoacid_CoA-transf_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="PTHR13707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02429">
        <property type="entry name" value="pcaI_scoA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="216" mass="23221" checksum="380D417F6F8F5BA1" modified="2009-02-10" version="1">MKIIDISEIKNLIKEGSTIMIGGFLGVGTPEKIIDEIIKNKIKNLTVIANDTAFEDKGIGKLVKNKLCKKVIVSHIGTNPETQRQMIEGTLQVELVPQGTLAERIRAGGVGLGGILTPTGVGTIVEEGKQIIEIEGKKYLLELPLRADVALIKAKKADYIGNLVFNLTAENFNPLMALSANTVIVEVEEIVPTGSLSPNEIKIPGVVVDYIVGVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID13</accession>
    <name>B7ID13_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101883.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1447"/>
    <dbReference type="EnsemblBacteria" id="ACJ75890">
        <property type="protein sequence ID" value="ACJ75890"/>
        <property type="gene ID" value="THA_1447"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1447"/>
    <dbReference type="eggNOG" id="COG1856">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067819_1_0_0"/>
    <dbReference type="OMA" id="IYECCVM"/>
    <dbReference type="OrthoDB" id="629114at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="12"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="246" mass="27943" checksum="8B716A209E5A5A8E" modified="2009-02-10" version="1">MVVVNPSGTLPISVTKGCPMNCKHCGGVYVKSMTHIDNIERYVNKYKSFLISGGMSKDGIIPFSPYLNKLKELKRTYNLQYNFHIGFPKEPPFEIIDVADVVSFDFFSDENVMKEVYTIRRTKDEILFSVLPLPVKKVPHITIGILCGKITHEEKSIEILKDYFDTIVLNILIPTKNTAYKDCNPPSVDEVAKVFNFAKKHFKSVILGCMHPHGSYRKQLLEKISPDVFVNSASKNYDFKGCCSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID31</accession>
    <name>B7ID31_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580241.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1465"/>
    <dbReference type="EnsemblBacteria" id="ACJ75908">
        <property type="protein sequence ID" value="ACJ75908"/>
        <property type="gene ID" value="THA_1465"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1465"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_0"/>
    <dbReference type="OMA" id="WRMGWII"/>
    <dbReference type="OrthoDB" id="554560at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="31"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="396" mass="44706" checksum="D78F04C63360857F" modified="2009-02-10" version="1">MISKRAQNVPASPIRRLVPYADAAKAKGIHVYHLNIGQPDIKTPKQWHEYIQKFKTEVVAYTHSQGIKPLREKFAEYYHKWNINVEPDEIMVTNGGSEAILFALGVVCDPGDEVIVIEPFYANYAGFAAYLNVKLVPVTAKPENGYRMPTMDDFEKVISPKTKAILFSNPSNPTGVVYTKEELEIIANIAKKFNLVVISDEVYREFTFDNTKAISMFEFEELRENNLIIVDSLSKRYSACGARIGTFVTKNKDFYAAALKLAQARLCPPEITQYGAIGLLNTDEEYMKNAMNEYRLRRDVAYDEISKIPGIIAHKPQGAFYLSAKLPVDDSEEFIKWMLSEFNVDGKTTMVAPLSGFYATKGLGKSEIRIAYVLESEKLKDAINIMAKAIEAYNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDC1</accession>
    <name>B7IDC1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamate formimidoyltransferase</fullName>
            <ecNumber evidence="2">2.1.2.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ftcD</name>
        <name evidence="9" type="ordered locus">THA_1560</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.5" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102241.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1560"/>
    <dbReference type="EnsemblBacteria" id="ACJ75998">
        <property type="protein sequence ID" value="ACJ75998"/>
        <property type="gene ID" value="THA_1560"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1560"/>
    <dbReference type="eggNOG" id="COG3643">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040037_0_0_0"/>
    <dbReference type="OMA" id="DEDHNRL"/>
    <dbReference type="OrthoDB" id="780971at2"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00555"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005542">
        <property type="term" value="F:folic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030409">
        <property type="term" value="F:glutamate formimidoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013802">
        <property type="entry name" value="Formiminotransferase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037070">
        <property type="entry name" value="Formiminotransferase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004227">
        <property type="entry name" value="Formiminotransferase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012886">
        <property type="entry name" value="Formiminotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037064">
        <property type="entry name" value="Formiminotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022384">
        <property type="entry name" value="FormiminoTrfase_cat_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02971">
        <property type="entry name" value="FTCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07837">
        <property type="entry name" value="FTCD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01221">
        <property type="entry name" value="FTCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01222">
        <property type="entry name" value="FTCD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55116">
        <property type="entry name" value="SSF55116"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02024">
        <property type="entry name" value="FtcD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0290">Folate-binding</keyword>
    <keyword evidence="5" id="KW-0369">Histidine metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FTCD_N" evidence="8">
        <location>
            <begin position="2"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="FTCD" evidence="7">
        <location>
            <begin position="180"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="301" mass="34495" checksum="5D251D1CCA960DFC" modified="2009-02-10" version="1">MKLIESVPNFSEGRREEVVRQIIEEAQKYERVKILDWSMDFDHNRSVVTLVGEPEEIETAIFDMVKKATELIDLRSHSGEHPRMGATDVIPFIPVMNTTMEECIEISKRVGERIGKELNIPVYLYEKSASSPDRENLAKIRKGEFEGFFEKIKDPKWKPDFGPDQVHPSAGVVAVGAREYLIAFNVNLGTNDIKIADKIAKAVRHISGGFRYVKAIGIELKEKGIVQVSMNLTNYKKSPIFRVFETIKREAQRYGVPVLNSEIIGMIPLKAAIETMKWYLQLDDFDEERVIENKILNTFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDD2</accession>
    <name>B7IDD2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Archease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1571</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102300.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1571"/>
    <dbReference type="EnsemblBacteria" id="ACJ76009">
        <property type="protein sequence ID" value="ACJ76009"/>
        <property type="gene ID" value="THA_1571"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1571"/>
    <dbReference type="eggNOG" id="COG1371">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163359_0_0_0"/>
    <dbReference type="OMA" id="WILEISK"/>
    <dbReference type="OrthoDB" id="1786857at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023572">
        <property type="entry name" value="Archease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036820">
        <property type="entry name" value="Archease_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01951">
        <property type="entry name" value="Archease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69819">
        <property type="entry name" value="SSF69819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Archease" evidence="5">
        <location>
            <begin position="2"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="125" mass="14603" checksum="59550016792FF26E" modified="2009-02-10" version="1">MYREIDHTADIAFEIVNDTFLGLLEDILKIIHDTYKVENINCNIAKVQEYDIKENEDGVFDIVNDWIAAIELGYFPVKIEKDKKYKTTFCSYSKIEGETFKALTYHNLKIEKKANKLSIKVVFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDD5</accession>
    <name>B7IDD5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lactose-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1574</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102306.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1574"/>
    <dbReference type="EnsemblBacteria" id="ACJ76012">
        <property type="protein sequence ID" value="ACJ76012"/>
        <property type="gene ID" value="THA_1574"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1574"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_2_4_0"/>
    <dbReference type="OMA" id="PWDSGPV"/>
    <dbReference type="OrthoDB" id="534010at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ76012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="416" mass="46964" checksum="2F418D76FAD7B213" modified="2009-02-10" version="1">MKKVLFVLLILSVLVSLGVAAKVTIWCWDPNFNVAIMQEAAERYKAMHPDVEFEIVSMAKADVEQKLNTILASGVKKGLPEIVLIEDYNAQKYLQSYPGSFADLTKKFNFKEFSPYKVKLMTLNGKVYGVPFDSGVAGWFYRRDYFKEAGIDESELRNITWDKFIELGKIVKEKTGKYMMAGDPYDGGLMRILLQSAGSWYFDKNGKPYIKDNPVLKEAVRLYKEIQDSGISKEVSGWNEWVAAFNNGDVASVITGVWIVGSIKAEKSQSGKWGVLPIPRLNLPGAVNASNLGGSSWYVLEHSENKDVAIDFLREIYAKDVDFYQKILIERGAVGSWIPAQNGPAYREPDPFFGYQRIYLDFTDWMDKIPPVDYGLFTYEADAALMGVMPDVYNGNLTIEKALEMAEKQFLNTIGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDH2</accession>
    <name>B7IDH2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flagellar export/assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_1611</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102378.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1611"/>
    <dbReference type="EnsemblBacteria" id="ACJ76049">
        <property type="protein sequence ID" value="ACJ76049"/>
        <property type="gene ID" value="THA_1611"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1611"/>
    <dbReference type="eggNOG" id="COG1317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102571_0_0_0"/>
    <dbReference type="OMA" id="YVYLDAP"/>
    <dbReference type="OrthoDB" id="1548186at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018035">
        <property type="entry name" value="Flagellar_FliH/T3SS_HrpE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02108">
        <property type="entry name" value="FliH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FliH" evidence="3">
        <location>
            <begin position="101"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="41"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="231" mass="26903" checksum="30208BA972AFAFB7" modified="2009-02-10" version="1">MIIKKRYVYIDTPQLIKSENVEKNKDLPSEDNKEIQKDILLEEAKKEAAKIVNEAKTNAKNIIESAKKEAEEIKNRALEEISQIKNKELEETNQKINNLNQKIISISNNFEEKLEKKVNELSNEMLYLIKTIIQKFLEKEIDEEVTKRKLEKVLMHIIGMKNVKLYLNPEDIKLLDEETLNHLRYRGIEIIEDNNVNSGVIAETESGTINTSLNFQIKLINEIIDEVLNNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDH3</accession>
    <name>ACKA_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetate kinase</fullName>
            <ecNumber evidence="1">2.7.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acetokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ackA</name>
        <name type="ordered locus">THA_1612</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP = acetyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:11352"/>
            <dbReference type="ChEBI" id="CHEBI:22191"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Mg(2+). Can also accept Mn(2+).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102380.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IDH3"/>
    <dbReference type="SMR" id="B7IDH3"/>
    <dbReference type="STRING" id="484019.THA_1612"/>
    <dbReference type="EnsemblBacteria" id="ACJ76050">
        <property type="protein sequence ID" value="ACJ76050"/>
        <property type="gene ID" value="THA_1612"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1612"/>
    <dbReference type="eggNOG" id="COG0282">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020352_0_1_0"/>
    <dbReference type="OMA" id="KIITCHI"/>
    <dbReference type="OrthoDB" id="537106at2"/>
    <dbReference type="UniPathway" id="UPA00340">
        <property type="reaction ID" value="UER00458"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008776">
        <property type="term" value="F:acetate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00020">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004372">
        <property type="entry name" value="Ac/propionate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000890">
        <property type="entry name" value="Aliphatic_acid_kin_short-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023865">
        <property type="entry name" value="Aliphatic_acid_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060">
        <property type="entry name" value="PTHR21060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00871">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000722">
        <property type="entry name" value="Acetate_prop_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00471">
        <property type="entry name" value="ACETATEKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00016">
        <property type="entry name" value="ackA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01075">
        <property type="entry name" value="ACETATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01076">
        <property type="entry name" value="ACETATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000116393" description="Acetate kinase">
        <location>
            <begin position="1"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="207"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="283"/>
            <end position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="331"/>
            <end position="335"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00020"/>
        </source>
    </evidence>
    <sequence length="406" mass="45155" checksum="2FF3F9F7CC0EB5D3" modified="2009-02-10" version="1">MIVLVVNSGSSSIKYQLLDMDNEKVLCKGLAERIGIPGSRIVHKKDGEKFVIEHPMANHDEALQLVLQTLKDEKMGAIKDFKEIDAVGHRVVHGGEKFSGSVLIDDEVINAIEEFSYLAPLHNPPNLMGIKAIMKLLPGVPNVAVFDTAFHAKMPKKAYLYAIPYEYYEKYKIRRYGFHGTSHRYVSRRTAEILGLDYNKSKIITVHLGNGASIAAVMNGKSVDTSMGFTPLEGLVMGTRSGDLDPSIVTFLIEKEGLSPEEVYTILNKKSGVLGLTSNFSSDMRDIEDKALENDPLCRLVLDIYEYRIAKYIGAYVAAMNGVDAISFTAGVGENSPITREEICENYLSYLGIKIDKEKNNVKGEERIISTPDSKVKVLIVPTNEELMIARDTKEIIEKGIKKLEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDI1</accession>
    <name>B7IDI1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Isoprenyl transferase</fullName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">uppS</name>
        <name evidence="3" type="ordered locus">THA_1620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580301.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1620"/>
    <dbReference type="EnsemblBacteria" id="ACJ76058">
        <property type="protein sequence ID" value="ACJ76058"/>
        <property type="gene ID" value="THA_1620"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1620"/>
    <dbReference type="eggNOG" id="COG0020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_1_1_0"/>
    <dbReference type="OMA" id="PRTEGHK"/>
    <dbReference type="OrthoDB" id="1630604at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="PTHR10291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="SSF64005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="55"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="178"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="225" mass="26652" checksum="9F8D1734FE6A0C68" modified="2009-02-10" version="1">MLNHIAFIMDGNGRWAKKQNKPRMYGHYAGAYKIEEVVRWCAEYGVKYTTFYAFSTENWKRPKGEVNFIFGLLQSKIGEFYERMNKEGVRLVFSGRLKELGEKIYNICMKYQEKTKNNDKIVVNMALNYGGRAEIVDAIKKIIDSKISDIDEEKFREFLYFPDIPDPDLIIRTSGEMRLSNFLTWQSAYSELYFTDVLWPDFSREDLDRAIEDFKKRQRRFGGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDN7</accession>
    <name>B7IDN7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal RNA small subunit methyltransferase G</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">16S rRNA 7-methylguanosine methyltransferase</fullName>
            <shortName evidence="2">16S rRNA m7G methyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gidB</name>
        <name evidence="2" type="synonym">rsmG</name>
        <name evidence="4" type="ordered locus">THA_1677</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically methylates the N7 position of a guanine in 16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102498.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1677"/>
    <dbReference type="EnsemblBacteria" id="ACJ76114">
        <property type="protein sequence ID" value="ACJ76114"/>
        <property type="gene ID" value="THA_1677"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1677"/>
    <dbReference type="eggNOG" id="COG0357">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065341_0_1_0"/>
    <dbReference type="OMA" id="AKINVIS"/>
    <dbReference type="OrthoDB" id="1590629at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070043">
        <property type="term" value="F:rRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00074">
        <property type="entry name" value="16SrRNA_methyltr_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003682">
        <property type="entry name" value="rRNA_ssu_MeTfrase_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31760">
        <property type="entry name" value="PTHR31760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02527">
        <property type="entry name" value="GidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003078">
        <property type="entry name" value="GidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00138">
        <property type="entry name" value="rsmG_gidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="2">
        <location>
            <begin position="80"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="2">
        <location>
            <begin position="108"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ76114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="212" mass="24490" checksum="AA645117F8F6B21D" modified="2009-02-10" version="1">MNFEKKQVILKYLKEIINAPLNLTAIKELELAYQLLAMDSLIPLRENDVGNNFLDVGTGGGVPGVFIGIMFENSRGLLVDSSRKKIDFVREVCNKLNLNNLEFLNARIEEKKDLIEKFDSVFSRAVAELRVILELTVPFSKVGGKIFLYKGKNYKEELDNAQNAIKELNVKLREIREYNILEKERVLLVFEKAKENDPKYPRRFNKILKQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDR4</accession>
    <name>B7IDR4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Had-superfamily phosphatase subfamily iiia</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580362.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1705"/>
    <dbReference type="EnsemblBacteria" id="ACJ76141">
        <property type="protein sequence ID" value="ACJ76141"/>
        <property type="gene ID" value="THA_1705"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1705"/>
    <dbReference type="eggNOG" id="COG2179">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056221_4_0_0"/>
    <dbReference type="OMA" id="MIGDQIF"/>
    <dbReference type="OrthoDB" id="1472357at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027706">
        <property type="entry name" value="PGP_Pase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010021">
        <property type="entry name" value="PGPP1/Gep4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF25">
        <property type="entry name" value="PTHR19288:SF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09419">
        <property type="entry name" value="PGP_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="150" mass="17771" checksum="60E04E0FE76291BA" modified="2009-02-10" version="1">MEIYPNVTSIDLKKLIDMGIDLFVFDFDNTINKWKESKIPNEIIQIFNYLEKNGAKIIIVSNGKPRNLNVNVKTLWLARKPLPFKFKKYLKEKNIKYNKAVVIGDQLFTDMLFGKLLNAYLIKVEPLDTQKEFFITKILRFFEKIVLKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDS8</accession>
    <name>B7IDS8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1719</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580370.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1719"/>
    <dbReference type="EnsemblBacteria" id="ACJ76155">
        <property type="protein sequence ID" value="ACJ76155"/>
        <property type="gene ID" value="THA_1719"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1719"/>
    <dbReference type="eggNOG" id="COG4828">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136765_1_0_0"/>
    <dbReference type="OMA" id="RRDINQF"/>
    <dbReference type="OrthoDB" id="2000842at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012427">
        <property type="entry name" value="DUF1622"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38468">
        <property type="entry name" value="PTHR38468"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07784">
        <property type="entry name" value="DUF1622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="110" mass="12121" checksum="B9FA5B46C4AC059C" modified="2009-02-10" version="1">MKFEEIIVNYLGLIFQLLAIIVITSGIVKAFTIFVKDALLKKDSKEAIEESRLEIGHSFSLGLAILIGSSILKTTVAPSWNDIGQLAAIIGIRTLLNYFLRKDIKGGDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDV8</accession>
    <name>B7IDV8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type IV pilus assembly protein PilZ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1751</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580388.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1751"/>
    <dbReference type="EnsemblBacteria" id="ACJ76185">
        <property type="protein sequence ID" value="ACJ76185"/>
        <property type="gene ID" value="THA_1751"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1751"/>
    <dbReference type="eggNOG" id="COG5581">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086342_0_1_0"/>
    <dbReference type="OMA" id="IAINDKC"/>
    <dbReference type="OrthoDB" id="1084216at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035438">
        <property type="term" value="F:cyclic-di-GMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009875">
        <property type="entry name" value="PilZ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009926">
        <property type="entry name" value="T3SS_YcgR_PilZN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07238">
        <property type="entry name" value="PilZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12945">
        <property type="entry name" value="YcgR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T3SS_YcgR_PilZN" evidence="2">
        <location>
            <begin position="22"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="PilZ" evidence="1">
        <location>
            <begin position="109"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="221" mass="25288" checksum="09F5B658A7C1B2CB" modified="2009-02-10" version="1">MASYVTNIPAEKALKIGLPGVVEITMVKELEGTYKTSIADMELSKKIIYLSIPTYKGRMIPIPKGVRMNVKIFDKSSMFSFTTVSLGVIKRDNLYMLPVLAPDEVKKTERRKFKRIPLYVYGILKKSLDENSEAIQFLTKDFSAGGIKFVTNTILKEGDIIYVTLNLDDELQIDNQKAKIVRVDQKTEEGYQYGAQFLEVPRQLENKMVRFVFQKEIKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDW1</accession>
    <name>B7IDW1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flagellar biosynthesis protein FlhF</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Flagella-associated GTP-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">THA_1754</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-binding SRP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580390.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1754"/>
    <dbReference type="EnsemblBacteria" id="ACJ76188">
        <property type="protein sequence ID" value="ACJ76188"/>
        <property type="gene ID" value="THA_1754"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1754"/>
    <dbReference type="eggNOG" id="COG1419">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_11_4_0"/>
    <dbReference type="OMA" id="WYEVDEY"/>
    <dbReference type="OrthoDB" id="1162441at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020006">
        <property type="entry name" value="FlhF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF3">
        <property type="entry name" value="PTHR43134:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03499">
        <property type="entry name" value="FlhF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="7" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0969">Cilium</keyword>
    <keyword evidence="12" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA" evidence="10">
        <location>
            <begin position="173"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="SRP54" evidence="11">
        <location>
            <begin position="174"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ACJ76188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="378" mass="42853" checksum="A5C3E57C4CC25EE2" modified="2009-02-10" version="1">MIVKKYVVKDIKEALEKIKIELGKDAVILGTRKIKKGGFLGIGAKTFLEVTAAVEEKEVEKQKQNENKQIYQLQELLSKNSKTTTSSSELSEIKKMMYELKTMVSSQKSDNEPEWSKDLRKSLSFHDVNFEIIEKIIEYIKIKYGSIDFNDENSRFVLSEVFLPFIKTEVPDLTGKVMFVGPTGVGKTTTLAKLAAKMSINNHKKVGILTLDTYRIAATEQLKTYATLMDIPMRVAYTPKEAKIELEAMADFDLVFIDTAGRSQKNDLQMNEIKAMSEIINPDFIFLVVGMQYRSNDVELITKKFSSVSPTHVILTKMDETSSLGHFLNIGHYINSPIIFVTNGQRVPDDIMEANNKELSIILSREVLNYVKSSKQFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE05</accession>
    <name>B7IE05_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphomethylpyrimidine kinase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1801</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580426.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1801"/>
    <dbReference type="EnsemblBacteria" id="ACJ76232">
        <property type="protein sequence ID" value="ACJ76232"/>
        <property type="gene ID" value="THA_1801"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1801"/>
    <dbReference type="eggNOG" id="COG0351">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1992">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035788_0_0_0"/>
    <dbReference type="OMA" id="AWIEIPA"/>
    <dbReference type="OrthoDB" id="461201at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045029">
        <property type="entry name" value="HMP/HMP-P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019293">
        <property type="entry name" value="ThiN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PTHR20858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10120">
        <property type="entry name" value="ThiP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phos_pyr_kin" evidence="1">
        <location>
            <begin position="11"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Phos_pyr_kin" evidence="1">
        <location>
            <begin position="137"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="402" mass="46017" checksum="9F0F960109795C94" modified="2009-02-10" version="1">MKKMLIISGLDPSSGAGLIQDISVATAMGVSIYSTVSAFTVQTLTKSLSVRFRDTNEILEEIKYFDDVGIIKVGIANPEIIEKLRDIYKDTIIVWNPVLQSSNGLKFLDENIVKNYIKLSDFVVVNSEEAEKIGKFDNMIITGGHLEDEKIKITYKGKEFYHERLNGNFRGTGCVFTTLFSCLVLKGYPAEEAIKEAGNIILKVLKRSSQRVEVEKLSSDWIKYETLDELNKIMLDIMEIGRYTIPEVGQNVSYAIMGAKNEEEVAKFPGRIRLVNDKPYFIGKATFEGKSHTARMTIEMMKKFPYMRCTTNVRYEEEYIENAKRYGLKVYELKREKEPEEFKNIEGQSLKWGINRIIENLDTPPDVIYDKGFWGKEAMIRVFARNPQEVIKKVRLIIGLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE24</accession>
    <name>B7IE24_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Processing protease, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1820</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1820"/>
    <dbReference type="EnsemblBacteria" id="ACJ76251">
        <property type="protein sequence ID" value="ACJ76251"/>
        <property type="gene ID" value="THA_1820"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1820"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_3_2_0"/>
    <dbReference type="OMA" id="WSNPDNV"/>
    <dbReference type="OrthoDB" id="1188251at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M16" evidence="3">
        <location>
            <begin position="25"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="4">
        <location>
            <begin position="168"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ76251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="424" mass="49186" checksum="3BFAE6408F76E0FE" modified="2009-02-10" version="1">MGDMMKKVVLSNGLEVYYYKIDGIRSVTIAFNVGVGSVYEPTNLLGISHFIEHLSFRGTEKYSMKELKLTVESVGGILNAWTDKENTVYYAKVPSSMAYETFDVLKEIVFYPVFKKEDLELEREIIYHEYLSNKEEPLNNLYELMFQEGIDGPHSKPVIGFEETIKSIGLDDIKEFHEEFYNPYNVKVIIVGHLPEEVFDKILEELEKIKRPGERTIKHKSIIKHGIIRRKIMKNANQVHILYVTDGFSLQETDRYAAIVLNTILSSGMSSYFFEEIREKEGLVYDIYTSNLAHKNWGLFNIYAATSIEKVQKFHEKMLNSINNFNLTDELFDYGIKRLIGKLELSTENTSALTTLIIEYISNEVDPELPNDIVSKIKNITKDKVNNVFEKLFSSKWSLFYVANENIDYFDKLGGIKFDYLEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE28</accession>
    <name>B7IE28_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1824</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580447.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1824"/>
    <dbReference type="EnsemblBacteria" id="ACJ76255">
        <property type="protein sequence ID" value="ACJ76255"/>
        <property type="gene ID" value="THA_1824"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1824"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018968_0_1_0"/>
    <dbReference type="OMA" id="VNDDPFY"/>
    <dbReference type="OrthoDB" id="1074175at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="514"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="550"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="583"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="642"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="682"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="515"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="718" mass="82825" checksum="187F6C105E59A138" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IE44</accession>
    <name>B7IE44_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PQQ enzyme repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1840"/>
    <dbReference type="EnsemblBacteria" id="ACJ76271">
        <property type="protein sequence ID" value="ACJ76271"/>
        <property type="gene ID" value="THA_1840"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1840"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_349476_0_0_0"/>
    <dbReference type="OMA" id="NTESTIW"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_beta_propeller_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="SSF50978"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="23"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="577"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="806" mass="93363" checksum="2C5D562E0D1808B8" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEA1</accession>
    <name>B7IEA1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004102920.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1902"/>
    <dbReference type="EnsemblBacteria" id="ACJ76328">
        <property type="protein sequence ID" value="ACJ76328"/>
        <property type="gene ID" value="THA_1902"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1902"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1872360_0_0_0"/>
    <dbReference type="OMA" id="WIDTITR"/>
    <dbReference type="OrthoDB" id="1606788at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="37"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ76328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="138" mass="15782" checksum="1BCD2159CA4D0A0C" modified="2009-02-10" version="1">MDYLLKLLNGNFVDKVTRLPNESFFNTVYSMLSNSNSTFFVITLDLDFDSLAEDEINFVIARAASVIKHSVRIPKDFVFRSGQKRFSIILHGINEITAKQIANRIKESLDYLFLNYKDKNIKIDTKIKIEKLGGAIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEG2</accession>
    <name>B7IEG2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1968</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103036.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1968"/>
    <dbReference type="EnsemblBacteria" id="ACJ76389">
        <property type="protein sequence ID" value="ACJ76389"/>
        <property type="gene ID" value="THA_1968"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1968"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_1_0"/>
    <dbReference type="OMA" id="VMIQLVQ"/>
    <dbReference type="OrthoDB" id="1074175at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="74"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="276" mass="32047" checksum="EF8BDFD0E5B61AB8" modified="2009-02-10" version="1">MKKTKKKKFPYITFTLLFLGSIIWIYPYFWLLFSSFKPTSEIYTRFWPTKFTLEHYKFIFLMSDKLQRPFFRALLNSIFISVTITFSVIVSSAFFGYILSKIRFKGRKGVFNFILFQMLFPGFMFIVPLFVLIKTLGLLNTYSAIILPSIISAWGSFMFAQTFKSIPNDYIEAAKIDGANTFWIVTRVMFPLARSTASIVGLFTFIGAWDNFMWPLIVMKDYNKMPLSVLLASFNHEYSSYVGPILAGSVIQTIPMVLIFLIFRKYFLQGISVSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEK4</accession>
    <name>B7IEK4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_10</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579292.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_10"/>
    <dbReference type="EnsemblBacteria" id="ACJ74518">
        <property type="protein sequence ID" value="ACJ74518"/>
        <property type="gene ID" value="THA_10"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_10"/>
    <dbReference type="HOGENOM" id="CLU_1937133_0_0_0"/>
    <dbReference type="OrthoDB" id="576504at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="130" mass="15180" checksum="D9CF52AF3AA1A36F" modified="2009-02-10" version="1">MVFKKTTFKYEIAVPFFGFIPDEIMPVWNFYTQNFYLSSYICPECGKLMMKTVFPNDYPFETNDGIKKVPRIFTCGDCKTLHIPAPGYKLSSNNGYYYKAKSDEEFEKIIKKIDKNGSLIGRQNTLYNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEL0</accession>
    <name>B7IEL0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_16</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103113.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_16"/>
    <dbReference type="EnsemblBacteria" id="ACJ74524">
        <property type="protein sequence ID" value="ACJ74524"/>
        <property type="gene ID" value="THA_16"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_16"/>
    <dbReference type="HOGENOM" id="CLU_211693_0_0_0"/>
    <dbReference type="OMA" id="KMSITGG"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="57" mass="6017" checksum="429F0ACA0449E2E2" modified="2009-02-10" version="1">MKRGEIVGATTLLGLGIGFFTKSSSHFVGSLLIGIGVGLLIDYITFVVSERKKKSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IES0</accession>
    <name>B7IES0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tetratricopeptide repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_76</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579335.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_76"/>
    <dbReference type="EnsemblBacteria" id="ACJ74584">
        <property type="protein sequence ID" value="ACJ74584"/>
        <property type="gene ID" value="THA_76"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_76"/>
    <dbReference type="eggNOG" id="COG1729">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_733489_0_0_0"/>
    <dbReference type="OMA" id="EYPFLKF"/>
    <dbReference type="OrthoDB" id="1619127at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="325"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="375" mass="44639" checksum="987B72D4721156AC" modified="2009-02-10" version="1">MNYNLIKFVRKLLKDRQFELAKNIIDIIEAEYPFLKFEYNLFIKNFEEALEIFKNLSHSDLQSLYNIENLENLNLEELLNKIYSSFDDNINYDEIKSEIPEISQLIIFELEKATKEKNRSNEQYLKDLLSKFEKNNYFLKEEKSNSSNFSIIFMSLLVVTLIFTIISLFNPVFFSIKNSINNIESNITSNYYNIKNELSKLNNVANEVFKNTSSLVESSQKMLEKDNSSTINNYFVELKNEIEKLKVQINYMSSKISTSSPLKNENSQTTLFEKFDPNSNETLRFMWLIAYQYYKQKNYIMAKNILETIIDKALSNNLNYLYFIDDVYYYRALIYYEMGDFENAYLLFNDFIERFPNSTYKKHAEYFIKILGGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEU7</accession>
    <name>B7IEU7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Germane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579349.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_103"/>
    <dbReference type="EnsemblBacteria" id="ACJ74611">
        <property type="protein sequence ID" value="ACJ74611"/>
        <property type="gene ID" value="THA_103"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_103"/>
    <dbReference type="eggNOG" id="ENOG502ZQYS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147456_0_0_0"/>
    <dbReference type="OMA" id="LNAYYFV"/>
    <dbReference type="OrthoDB" id="1826291at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019606">
        <property type="entry name" value="GerMN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10646">
        <property type="entry name" value="Germane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00909">
        <property type="entry name" value="Germane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Germane" evidence="1">
        <location>
            <begin position="46"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="152" mass="17420" checksum="4629DFF057F0A9ED" modified="2009-02-10" version="1">MKRIITLLFILIASFTLVASKIAFIENNGIILLEVGEFSSNKVVAVQEIFEKFSKVKLSGNQKNFVPQGILNAYYFVDTALIIDLNSKNIQNYSSEEEIFLLLQILYSLFENINGIDRIYILVDGKQSEIFIRSVNIYFSFPKDLYKTKKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEX7</accession>
    <name>B7IEX7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_133"/>
    <dbReference type="EnsemblBacteria" id="ACJ74641">
        <property type="protein sequence ID" value="ACJ74641"/>
        <property type="gene ID" value="THA_133"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_133"/>
    <dbReference type="HOGENOM" id="CLU_3334138_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="38" mass="4658" checksum="42566FAE20AF06D4" modified="2009-02-10" version="1">MFLEQKNVIILLTKGDNKMFKELLHYKFKLEFSQRLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEY1</accession>
    <name>B7IEY1_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAGPA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579370.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_137"/>
    <dbReference type="EnsemblBacteria" id="ACJ74645">
        <property type="protein sequence ID" value="ACJ74645"/>
        <property type="gene ID" value="THA_137"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_137"/>
    <dbReference type="eggNOG" id="COG4632">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035800_0_0_0"/>
    <dbReference type="OMA" id="VTKFPIG"/>
    <dbReference type="OrthoDB" id="1587976at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018711">
        <property type="entry name" value="NAGPA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09992">
        <property type="entry name" value="NAGPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAGPA" evidence="1">
        <location>
            <begin position="363"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="535" mass="61517" checksum="449C6D25F069B7D1" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEY8</accession>
    <name>B7IEY8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fibronectin-binding protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579373.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_144"/>
    <dbReference type="EnsemblBacteria" id="ACJ74652">
        <property type="protein sequence ID" value="ACJ74652"/>
        <property type="gene ID" value="THA_144"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_144"/>
    <dbReference type="eggNOG" id="COG1293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022481_2_1_0"/>
    <dbReference type="OMA" id="SKPGFVI"/>
    <dbReference type="OrthoDB" id="713667at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT-R_1" evidence="1">
        <location>
            <begin position="443"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="554" mass="66089" checksum="A75FD26B671DDA26" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEZ5</accession>
    <name>B7IEZ5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA integrity scanning protein DisA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">THA_152</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP = 3',3'-c-di-AMP + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:35655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:71500"/>
            <dbReference type="EC" id="2.7.7.85"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_152"/>
    <dbReference type="EnsemblBacteria" id="ACJ74659">
        <property type="protein sequence ID" value="ACJ74659"/>
        <property type="gene ID" value="THA_152"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_152"/>
    <dbReference type="eggNOG" id="COG1623">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_787128_0_0_0"/>
    <dbReference type="OMA" id="VVRDYVP"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106408">
        <property type="term" value="F:diadenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038331">
        <property type="entry name" value="DisA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036888">
        <property type="entry name" value="DNA_integrity_DisA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018906">
        <property type="entry name" value="DNA_integrity_scan_DisA_link"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003390">
        <property type="entry name" value="DNA_integrity_scan_DisA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02457">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10635">
        <property type="entry name" value="DisA-linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143597">
        <property type="entry name" value="SSF143597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51794">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAC" evidence="7">
        <location>
            <begin position="4"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ74659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="357" mass="40542" checksum="C02982D2A43D89A0" modified="2009-02-10" version="1">MSIPQELISKIKLLAPGTKLRKALDDIVLANFGALIVFISEEKYEKYSDIFQAGFKLEIPFLPEKLYELAKMDGAIIIDDNVTKILAANVHLVPDPSIPTSETGTRHRTAERVAKQLGELTIAVSKRRNVISLYYGSYKYIINDINFLITRVNQALNTLEKYRQNFDKMILNLDVLEIENRVNLIDVVEIINKGIEILKIKEEVEPYVIELGVEGRLASMQLLEIIVEMEDILKNLILDYSKEDIDEEDEAMQILEVLKGYKERKPISTARLLGYEDIANINQLNDYHVQSRGYRLLRNVAKIPMTITHNVVKAFGNVFSLSNADFSALKEVEGIGEKRASAIIDSINSMRKRISQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF00</accession>
    <name>B7IF00_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103616.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_157"/>
    <dbReference type="EnsemblBacteria" id="ACJ74664">
        <property type="protein sequence ID" value="ACJ74664"/>
        <property type="gene ID" value="THA_157"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_157"/>
    <dbReference type="HOGENOM" id="CLU_2304695_0_0_0"/>
    <dbReference type="OMA" id="ETAIKME"/>
    <dbReference type="OrthoDB" id="2211241at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037285">
        <property type="entry name" value="TM0693-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140560">
        <property type="entry name" value="SSF140560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="100" mass="11385" checksum="F230D78F8336C1EA" modified="2009-02-10" version="1">MADEEIIKIEEMIDKAIEEENFQLLNELLEKRENLLKKGISEELAKLILEKDKQRKNKILEMMEQLGVQAKNIKLGEKALKGYGGIEGLNNTGGRFKGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF05</accession>
    <name>B7IF05_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyl-accepting chemotaxis protein McpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103625.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_162"/>
    <dbReference type="EnsemblBacteria" id="ACJ74669">
        <property type="protein sequence ID" value="ACJ74669"/>
        <property type="gene ID" value="THA_162"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_162"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_18_0"/>
    <dbReference type="OMA" id="QNLMLSF"/>
    <dbReference type="OrthoDB" id="477199at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="Methyl-accepting transducer" evidence="2">
        <location>
            <begin position="28"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="279" mass="31236" checksum="9E38FA78699B0173" modified="2009-02-10" version="1">MKIDEKTLNTLSSAFYVQNLMLSFMAQLDEVLVSRINDIKDDIEKITSRFEELNDNIFKLLESFSKESKSIVENVEYATEVNQKINDDLKSLGTDVDNVSLKFKDTIGKTSETFEKFSKVEELVKAIQRIAKQTNLLALNASIEAARAGEYGKGFSVVASEIQKLAEESQETSANISKIISEISNSVNESINSLKQIEGLFSAVQNSLSEALNYMKKNIQVLVSSKKLYEDTSESLENEYKLLSNAVEILGETSKEFNTISEVIRSIVKAQNSLKNLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF12</accession>
    <name>B7IF12_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Diaminopimelate epimerase</fullName>
            <shortName evidence="8">DAP epimerase</shortName>
            <ecNumber evidence="4 8">5.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">PLP-independent amino acid racemase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">dapF</name>
        <name evidence="10" type="ordered locus">THA_171</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(2S,6S)-2,6-diaminoheptanedioate = meso-2,6-diaminoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:15393"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="5.1.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the diaminopimelate epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.7" evidence="4 8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579387.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_171"/>
    <dbReference type="EnsemblBacteria" id="ACJ74676">
        <property type="protein sequence ID" value="ACJ74676"/>
        <property type="gene ID" value="THA_171"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_171"/>
    <dbReference type="eggNOG" id="COG0253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053306_3_2_0"/>
    <dbReference type="OMA" id="CGNGART"/>
    <dbReference type="OrthoDB" id="1921631at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00025"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008837">
        <property type="term" value="F:diaminopimelate epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00197">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018510">
        <property type="entry name" value="DAP_epimerase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001653">
        <property type="entry name" value="DAP_epimerase_DapF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689">
        <property type="entry name" value="PTHR31689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01678">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00652">
        <property type="entry name" value="DapF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01326">
        <property type="entry name" value="DAP_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="68"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="181"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="192"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ74676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="237" mass="26547" checksum="1CB83C1DE88AF64D" modified="2009-02-10" version="1">MIVEKFTATGNSFIVCDILGSKLNDKEKSNFVLKNSKDRDGVIFVEKRDGQFFMDYFNKDGNRAAFCGNGARTFLYYLMKKGYVKEDLVKFYTYAGEITGKLTKNGVMIKMPASNMPQKLTIDSFSGFLLNVGVPHFVIFVDNVETIDVNKIGKQLREKLNSNINFVQVLDEKTIKIRTFERGVEAETLACGSGTTASAYVFNKSNLEKIEVKARGGTLFVHFLNDGIYLEGGVENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IF63</accession>
    <name>B7IF63_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphate acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphate acetyltransferase and butyryltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103759.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_222"/>
    <dbReference type="EnsemblBacteria" id="ACJ74727">
        <property type="protein sequence ID" value="ACJ74727"/>
        <property type="gene ID" value="THA_222"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_222"/>
    <dbReference type="eggNOG" id="COG0280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056531_0_0_0"/>
    <dbReference type="OMA" id="NPDMPET"/>
    <dbReference type="OrthoDB" id="1301815at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012147">
        <property type="entry name" value="P_Ac_Bu_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002505">
        <property type="entry name" value="PTA_PTB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01515">
        <property type="entry name" value="PTA_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000428">
        <property type="entry name" value="P_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PTA_PTB" evidence="2">
        <location>
            <begin position="73"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ74727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="294" mass="30974" checksum="761249FB99B5B4B7" modified="2009-02-10" version="1">MLRSFDEVIEKAKTKEAVIALAGAEDVESLKAIKDVLDLGIKAILVGKEEIIRKNLEGLEMELPIVNAESEAEASEKAVKLVSSGQANVLMKGLVKTATLLKAALNKEWGLRGEGLLSHVALLETPGMDRIVFVTDGGMVIKPTLEQKVQIIKNAVSLAHSLGYEEPKVGLIAAVEVVNPDMPETMEAAILTKMADRGQIKGCKIDGPLGLDNALSEFAAKVKKVSGPVAGKADILVVPDIHSGNFLGKSAVYLANGKIAGIIMGAKVPIIIVSRADTSESKRNSIAIAAAISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFA3</accession>
    <name>B7IFA3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">SufB/sufD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_266</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579457.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_266"/>
    <dbReference type="EnsemblBacteria" id="ACJ74767">
        <property type="protein sequence ID" value="ACJ74767"/>
        <property type="gene ID" value="THA_266"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_266"/>
    <dbReference type="eggNOG" id="COG0719">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026231_2_0_0"/>
    <dbReference type="OMA" id="WGFPKWK"/>
    <dbReference type="OrthoDB" id="422804at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011542">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43575">
        <property type="entry name" value="PTHR43575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="SSF101960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="374" mass="42672" checksum="A8A63F3BB8CBB1EE" modified="2009-02-10" version="1">MEKNLELFLDNEKAVVSVPKQFFEGDYTEKDLERALSSIENKFVRKYVLEKYSEYTKIGFPIWKRLKINSLSLPEYKYEGYFKGVEEDLDLIDFEGTHRKYVLLSDIFSSMGEYIKVDNEKDVKVEYIDRITNDVYKVNGKLVLVRILKTNNFSNNTLRVVAEDNAEVEIYNIHVSEENSFSVDNIFIMVEDKVQVKVRDIYIGGGKVAGYLGVKMNGNESNVDVKPYFLGSKSAIFDLQYLLRFVGLENKGSIKAEGALSDEAKVVFRGILDLKRGAKNSEAEEMERCILLSKDSKMEAIPSLLVDENEVTASHAASSAPLDEDAIFYLMSRGFNRKEAKSFILNGIFENLINELSVFGLEEVVKNALKKYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFE7</accession>
    <name>B7IFE7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cmr crispr-associated ramp protein, Cmr4 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579490.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_311"/>
    <dbReference type="EnsemblBacteria" id="ACJ74811">
        <property type="protein sequence ID" value="ACJ74811"/>
        <property type="gene ID" value="THA_311"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_311"/>
    <dbReference type="eggNOG" id="COG1336">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047795_0_0_0"/>
    <dbReference type="OMA" id="FAWITCP"/>
    <dbReference type="OrthoDB" id="972874at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013410">
        <property type="entry name" value="CRISPR-assoc_RAMP_Cmr4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36700">
        <property type="entry name" value="PTHR36700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02580">
        <property type="entry name" value="cas_RAMP_Cmr4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="294" mass="33765" checksum="344EAE1995C57FA9" modified="2009-02-10" version="1">MNHEVGRIGFFYAVTQIHAGKGFDVGIVDQPIQREIHTRFPVINGVKGAIRNEISRLININYNDITQIFGTEFQDNEASEAGKVSFSEGKLAFYPVRSIDRGIVWITCPLILSRLKIAFDVVGLEEFKKLIEKINISYKEDYKALSTINDSKICLEEFEQDIKYSKDLMSFVNHLRNIFPDERLFERFSSNLVILSDKDFSYFVTNSTEVIARIRINPQKRTVDKGALWYEEYIPQDSVLFFISKLLVNNADYLLKKIDELNSKFLNIGGNASIGKGIVYISFLPFKGEDKNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFG3</accession>
    <name>B7IFG3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">AstB/chuR-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_327</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579502.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_327"/>
    <dbReference type="EnsemblBacteria" id="ACJ74827">
        <property type="protein sequence ID" value="ACJ74827"/>
        <property type="gene ID" value="THA_327"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_327"/>
    <dbReference type="eggNOG" id="COG0535">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_1_0"/>
    <dbReference type="OMA" id="GWGRQFF"/>
    <dbReference type="OrthoDB" id="1172757at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017200">
        <property type="entry name" value="PqqE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037420">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="316" mass="36047" checksum="B40B301668BD8B67" modified="2009-02-10" version="1">MRYPKIIEFELTTACNYSCKHCYCNAGKKSTNELSFDEVKKVLHDLKDANVEIVDLIGGEPLIRQDIIDIISYAASIGLDVMLNTNASLASKEIVKRIKEVYPQLKVGISFDGHTPEIHDFVRGPGAFEKTYRGFLNFLEEGFDVTVLHVVNKVNYEHFEEMVKFAMEHNVSLYVDRFVPVGRGELYKDLLLPTKKEVEHVRKVIKKYENKLTFYVEENISGGLCTAGREHASVLVDGTVVPCGHFRYDKEYYMGNLKEKPFKQIWESYNPDILISNCKGCPHFSNCFGGCRAFAKKLGLDYDPIFCEVHVDDKEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-22" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFG6</accession>
    <name>RPOZ_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit omega</fullName>
            <shortName evidence="1">RNAP omega subunit</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase omega subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit omega</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoZ</name>
        <name type="ordered locus">THA_330</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase subunit omega family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004103971.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFG6"/>
    <dbReference type="SMR" id="B7IFG6"/>
    <dbReference type="STRING" id="484019.THA_330"/>
    <dbReference type="EnsemblBacteria" id="ACJ74830">
        <property type="protein sequence ID" value="ACJ74830"/>
        <property type="gene ID" value="THA_330"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_330"/>
    <dbReference type="eggNOG" id="COG1758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125406_4_0_0"/>
    <dbReference type="OMA" id="YKFAIPI"/>
    <dbReference type="OrthoDB" id="1904357at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00366">
        <property type="entry name" value="RNApol_bact_RpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003716">
        <property type="entry name" value="DNA-dir_RNA_pol_omega"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="SSF63562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00690">
        <property type="entry name" value="rpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000205532" description="DNA-directed RNA polymerase subunit omega">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00366"/>
        </source>
    </evidence>
    <sequence length="75" mass="8765" checksum="F4B98F1760E85056" modified="2009-02-10" version="1">MRPVINYDELLKKIPYKFAIPIAAAKRAENLKEFAKPYVETWDNNYVSIALKELSEGYIRIKNEEILKVLIPNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFH4</accession>
    <name>B7IFH4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_338</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579509.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_338"/>
    <dbReference type="EnsemblBacteria" id="ACJ74838">
        <property type="protein sequence ID" value="ACJ74838"/>
        <property type="gene ID" value="THA_338"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_338"/>
    <dbReference type="eggNOG" id="COG1100">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084710_0_0_0"/>
    <dbReference type="OMA" id="VANIYRP"/>
    <dbReference type="OrthoDB" id="1370117at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="214" mass="24037" checksum="BF1697279253702C" modified="2009-02-10" version="1">MAKNFVFVGLFGSGKTEVAINYALMLKNEHENVAIADVDIISPYFRTRDAIDELEAEGIKVVTPPGALKHADLPIVTGAVAGYLNNPQYKTVLDVGGEENGIVVVGYLKPHLKDTEIYMVVNTRRPFTSTVDGIVKTYKQLTEVAKIKIDYLINNSNLSSETTKEVILEGERILKEASQVLEVPVKYTVIPDFLEDFETEFPKFRLKRFMKMDF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFM4</accession>
    <name>GLMU_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional protein GlmU</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">UDP-N-acetylglucosamine pyrophosphorylase</fullName>
                <ecNumber evidence="1">2.7.7.23</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">N-acetylglucosamine-1-phosphate uridyltransferase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Glucosamine-1-phosphate N-acetyltransferase</fullName>
                <ecNumber evidence="1">2.3.1.157</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">glmU</name>
        <name type="ordered locus">THA_395</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate + UTP = diphosphate + UDP-N-acetyl-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.23" evidence="1"/>
    <dbReference type="EC" id="2.3.1.157" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579545.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFM4"/>
    <dbReference type="SMR" id="B7IFM4"/>
    <dbReference type="STRING" id="484019.THA_395"/>
    <dbReference type="EnsemblBacteria" id="ACJ74888">
        <property type="protein sequence ID" value="ACJ74888"/>
        <property type="gene ID" value="THA_395"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_395"/>
    <dbReference type="eggNOG" id="COG1207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_15_2_0"/>
    <dbReference type="OMA" id="TAIVEHK"/>
    <dbReference type="OrthoDB" id="1381953at2"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00533"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00973"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03353">
        <property type="entry name" value="LbH_GlmU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01631">
        <property type="entry name" value="GlmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005882">
        <property type="entry name" value="Bifunctional_GlmU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038009">
        <property type="entry name" value="GlmU_C_LbH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01173">
        <property type="entry name" value="glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000186504" description="Bifunctional protein GlmU">
        <location>
            <begin position="1"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyrophosphorylase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Linker" evidence="1">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="239"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="6"/>
            <end position="9"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="73"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="95"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="373"/>
            <end position="374"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01631"/>
        </source>
    </evidence>
    <sequence length="451" mass="50180" checksum="BBA68F13C2017FE6" modified="2009-02-10" version="1">MKTLILAAGLGKRMNSKYPKVIHKILGKPMINWVIDTAKSFGEVGVVLGHKHEMVEKVIPQDVKIFLQNEQLGTAHAVMCGFDFIPEKDNLLILYGDVPFISYETLKRLEKEHIESNSDVTILTAILENPAGYGRIVRGKKIEIVEDKDADEKIKKIKEINTGIYIFKGKFLKENIKKIKNDNAQKEYYLTDILKFTENISTVTTDDIDEVTGVNDRIQLSKLEKNMRKRINEKLMREGVRIIDPESVYIDITVKIGKDTIIYPFTFIEGETEIGEDCVIGPMTRIKDSKIGNNVNVIRSEVEKAIIEDNVSVGPFSRLREGTHLKSNVKIGNFVETKKSVIGKNTKAQHLTYLGDATIGENVNIGAGTITCNYDGVKKHPTIIEDGAFIGSNNSLVAPVKIGKNAITGAGSTITEDVPENSLGLGRARQVVIKDWVLRKKGGNQNADSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFN5</accession>
    <name>B7IFN5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_406</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579554.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_406"/>
    <dbReference type="EnsemblBacteria" id="ACJ74899">
        <property type="protein sequence ID" value="ACJ74899"/>
        <property type="gene ID" value="THA_406"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_406"/>
    <dbReference type="HOGENOM" id="CLU_936683_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="297" mass="32432" checksum="C104E6E8F944830C" modified="2009-02-10" version="1">MKKFLVLLVSLVVLGVVFADVTFSGGVDVSYTLESSPLKISDSVSLSWDRIEMTASGDGYYLRVIYDSSNSFAIDQAYMTFSFDPVTLYVGKKGYWLNPYAFTDGNSLTWNYSGVSWYYPKEYLGAKVSFDNGSVYMTLDPRYAEGSATPSVNVFGNVSFDPVSLGFIAQDNFKELGLGVTVNVAPVTASAAFGYSIENSTITALLVGVNGTFDPVSVLGEVDLTDFNAIVVNGIVNYTINDKMDAGLNAKYNLGDDKSFGTSAWFNYYYNDNITLTPSVSFDGEVITLNMNAHFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFN7</accession>
    <name>B7IFN7_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tyrosyl-tRNA synthetase</fullName>
            <shortName evidence="8">TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">tyrS</name>
        <name evidence="11" type="ordered locus">THA_408</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + H(+) + L-tyrosyl-tRNA(Tyr)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="8"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579556.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_408"/>
    <dbReference type="EnsemblBacteria" id="ACJ74901">
        <property type="protein sequence ID" value="ACJ74901"/>
        <property type="gene ID" value="THA_408"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_408"/>
    <dbReference type="eggNOG" id="COG0162">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024003_5_0_0"/>
    <dbReference type="OMA" id="YMMAKDS"/>
    <dbReference type="OrthoDB" id="402899at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02007">
        <property type="entry name" value="Tyr_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024108">
        <property type="entry name" value="Tyr-tRNA-ligase_bac_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="PTHR11766"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766:SF1">
        <property type="entry name" value="PTHR11766:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="10">
        <location>
            <begin position="347"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="225"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ACJ74901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="398" mass="46207" checksum="82403981BC5453CB" modified="2009-02-10" version="1">MDFSKQLEILKKNVVDFVSEEELVERLKENRPLRVKLGVDPSRPDLHLGHAVVLKKLKQFQELGHHIILIIGDFTARIGDPTGRNSTRPMLSEEEVRKNAETYANQAFKILDKEKTEIRFNNEWLGKMKFEDVVKLAGKYTVARMLERDDFAKRLSQGYPISIAEFLYPLAQAYDSVAIQADVELGGTDQLFNLLVGRKIQEEYGQKPQIVMTMPIIEGTDGKLKMSKSYDNYIAFTDEPNDMYGKIMSIPDELIAKYMRLITDIEESEIEEYEILMKEGKINPRDVKMRLAREIVAFFYDKEKARMAEENFVKVFRQKEIPDEMPEIQVEPGEYNIVDLVTKVNSNYSKSEVKRLISQGGVYFDGKRVNDFKQIVKVEKEHVLRLGKRKFFKLITKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFP3</accession>
    <name>B7IFP3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_414</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579561.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_414"/>
    <dbReference type="EnsemblBacteria" id="ACJ74907">
        <property type="protein sequence ID" value="ACJ74907"/>
        <property type="gene ID" value="THA_414"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_414"/>
    <dbReference type="eggNOG" id="ENOG502ZXQ1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1642910_0_0_0"/>
    <dbReference type="OMA" id="EFEPDIW"/>
    <dbReference type="OrthoDB" id="1786678at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="161" mass="18651" checksum="A42273EA975C4300" modified="2009-02-10" version="1">MRILLSNSFLVEEFEPDIWVTSDFENLQIPYTLFYNGQAVFIYDHFKFVDTILNNSEKIIDFGFFKAGILVGSSISVAENYLKDVELFIAFDKTNYTSKFLLRKAQTIVASDISGKTILSLFIYKDKTNYIKTLPNIGIVDDSMSHVLWEKEFKESKRVEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFS1</accession>
    <name>UPPP_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Undecaprenyl-diphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Bacitracin resistance protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl pyrophosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uppP</name>
        <name type="ordered locus">THA_444</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>di-trans,octa-cis-undecaprenyl diphosphate + H2O = di-trans,octa-cis-undecaprenyl phosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:28094"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="EC" id="3.6.1.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UppP family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.27" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579584.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IFS1"/>
    <dbReference type="SMR" id="B7IFS1"/>
    <dbReference type="STRING" id="484019.THA_444"/>
    <dbReference type="EnsemblBacteria" id="ACJ74935">
        <property type="protein sequence ID" value="ACJ74935"/>
        <property type="gene ID" value="THA_444"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_444"/>
    <dbReference type="eggNOG" id="COG1968">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060296_1_2_0"/>
    <dbReference type="OMA" id="IEGPFRD"/>
    <dbReference type="OrthoDB" id="1826154at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050380">
        <property type="term" value="F:undecaprenyl-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016311">
        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01006">
        <property type="entry name" value="Undec_diphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003824">
        <property type="entry name" value="UppP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622">
        <property type="entry name" value="PTHR30622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02673">
        <property type="entry name" value="BacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="SSF118215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0046">Antibiotic resistance</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_1000197417" description="Undecaprenyl-diphosphatase">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01006"/>
        </source>
    </evidence>
    <sequence length="248" mass="27461" checksum="86BCDDF5DA29A534" modified="2009-02-10" version="1">MNHIVLGLIQGLTEFLPISSSGHLTLFSYLFNIEPNISNFAFLHLATLAAIIVFVWKEIVEILKGLFTLKKEYYSLVLKIIISTIPAAIFGFLFNSTIENSFSNLKIISFFFLVTAASLFVSDKLKGKKDFFNISYIDALIIGLFQMIAIFPGISRSGITLFGALTVGLEREKALKYSFLMGIPVILGAGILETSKIELNSYILISGLVAFLSGLLSLLILKKLTISKKLKIFSYYCILIAIIAFFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFU4</accession>
    <name>B7IFU4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongation factor 4</fullName>
            <shortName evidence="5">EF-4</shortName>
            <ecNumber evidence="5">3.6.5.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ribosomal back-translocase LepA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">lepA</name>
        <name evidence="7" type="ordered locus">THA_467</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.n1" evidence="5"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104247.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_467"/>
    <dbReference type="EnsemblBacteria" id="ACJ74958">
        <property type="protein sequence ID" value="ACJ74958"/>
        <property type="gene ID" value="THA_467"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_467"/>
    <dbReference type="eggNOG" id="COG0481">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009995_3_3_0"/>
    <dbReference type="OMA" id="MVQIAIQ"/>
    <dbReference type="OrthoDB" id="182107at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="PTHR43512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="6">
        <location>
            <begin position="2"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="132"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ74958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="603" mass="67664" checksum="6839DE14E0B322EF" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFU9</accession>
    <name>B7IFU9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_472</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579601.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_472"/>
    <dbReference type="EnsemblBacteria" id="ACJ74963">
        <property type="protein sequence ID" value="ACJ74963"/>
        <property type="gene ID" value="THA_472"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_472"/>
    <dbReference type="eggNOG" id="ENOG502ZSZX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1359857_0_0_0"/>
    <dbReference type="OMA" id="TKNGWIS"/>
    <dbReference type="OrthoDB" id="1870037at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ74963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="201" mass="22808" checksum="E5D250DCD8D77684" modified="2009-02-10" version="1">MRRLSVFLILSIFILSFSSVYNIKIGETSFGTSVLIKVDPQTYRSISTIDYGVVYTVDATTVYDGLWFKSYVAAFTVDGSFTGVLKAENNGNEINFVYETRYGSKTSYKLSGGKFVILDNNFILAHIERLLEYPIPYFKLVVPQLIFNPSKTEFATGQASLKKNGEIFEIKFQDESIKIKYENGKIISLEYPGFVKVELKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFV9</accession>
    <name>B7IFV9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lysophospholipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_482</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579609.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_482"/>
    <dbReference type="ESTHER" id="theab-b7ifv9">
        <property type="family name" value="Monoglyceridelipase_lysophospholip"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ74973">
        <property type="protein sequence ID" value="ACJ74973"/>
        <property type="gene ID" value="THA_482"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_482"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026209_7_2_0"/>
    <dbReference type="OMA" id="FHGFSDH"/>
    <dbReference type="OrthoDB" id="1694750at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="10"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="256" mass="29181" checksum="B02B999CF6F4BF11" modified="2009-02-10" version="1">MIYSIKKGEPKKGWVVVVHGLGEHIGRYEKLIDGLAERGYAVLGFDLPGHGKSSGKRGHTSIEEVISVINDLTRENNIEKFHIFGHSLGGLISIRYTQENLQRVRSLVVSSPALFLSPKFSQLLLLNLFSIIYPALTLRNGIDPKDLSRNEEAVEKYITDDLVHDKISIKLAKSLLKNVKLAHEKVGIISVPTMMLVGTADKVTPPQGSYLFFNNLQLMKENKKLVRFEGAFHEIFEDIEWSEEFYFTIFEWYEMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFX3</accession>
    <name>B7IFX3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MoxR protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104343.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_497"/>
    <dbReference type="EnsemblBacteria" id="ACJ74987">
        <property type="protein sequence ID" value="ACJ74987"/>
        <property type="gene ID" value="THA_497"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_497"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018678_0_0_0"/>
    <dbReference type="OrthoDB" id="895310at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045427">
        <property type="entry name" value="MoxR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20030">
        <property type="entry name" value="bpMoxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="47"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="321" mass="37928" checksum="27C518460DB28BDE" modified="2009-02-10" version="1">MKKDRTSGNFRELTRMFKNYLEVIYTLKNIILNKDDVIDAISCGFLTQTNVLLIGEPGTAKSLVINEFAKLMGFDIENGYFHYLLTKFTEPSEVLGPLNISELKKGKYVINTKNKLPEANVVFLDEVFNANSAILNSLLTLINEKKLLLGDIYKSLDDLIVIYGATNHTPTDPLLKAFYDRFPIRVLVEGVERKDYEELLDREFDIEEKNLRKDKEILFDRKESIMFSKMMNEYIMMKYFELRQNKYITDLLDRIEMLKKDKDIFISDRNLKFYVKNMIAYSMIRNESLNPNLNLEDLKFILSKIFNNEEQKYEIERYFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFZ6</accession>
    <name>B7IFZ6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyruvate/ketoisovalerate oxidoreductases common subunit gamma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_521</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100654.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_521"/>
    <dbReference type="EnsemblBacteria" id="ACJ75010">
        <property type="protein sequence ID" value="ACJ75010"/>
        <property type="gene ID" value="THA_521"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_521"/>
    <dbReference type="eggNOG" id="COG1014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_2_0_0"/>
    <dbReference type="OMA" id="PDYVIVQ"/>
    <dbReference type="OrthoDB" id="1777574at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="14"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="191" mass="20942" checksum="0BF16DB73C7A4D05" modified="2009-02-10" version="1">MPAKYFEIRWHARAGQGAKSASQMLAEAALEMGKYVQSFPEYGAERTGAPMKAFNRVADEPILVHSSVDNPDVVVIIDDTMLGQPMLTEGTDENTVFIVNTVKDVEYVRQKLGAKGKVCTIAATDIALEEIKRGIPNTVMLGAIAKVTGIITLESVEQRIKKAFAKKFPEEVVEANVRALRRGFEEVKCNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG01</accession>
    <name>B7IG01_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579630.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_527"/>
    <dbReference type="EnsemblBacteria" id="ACJ75015">
        <property type="protein sequence ID" value="ACJ75015"/>
        <property type="gene ID" value="THA_527"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_527"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_92_13_0"/>
    <dbReference type="OMA" id="LWSKVAQ"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD-GYP" evidence="2">
        <location>
            <begin position="219"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="241"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="405" mass="45858" checksum="99EE2D5CCC126E48" modified="2009-02-10" version="1">MYAPYWVSGYIIDKEIIGKKSKIKVNIDGIEFYLLEKDINYSILKMLVRHAKKKSLKILDDVEKVSFMAAKLNSSLRLSTLLKNIQESICNILDAEAASVLLLKDDHLQFLVTVGKASGKIESIPVPMESIAGTIYKSGKAMVFNDLSKAPHFKKVDKVSKFKTKNIVGAPIYSDQEKVGVIEVLNKEDGFNDRDAKIVELFAKLIGKKLLSTWEHEKFSKLFKNIILAFSTMIDKRDKYTHMHSKNVAYISRLIGEELGFSEHTLEQLEYSAILHDVGKIGIPDSLLLKKGKLTDEEYKIIQSHTIMGAEILEEIKHTTYDIISGALEHHERLDGTGYPYGKKNGEISLFGRIIAIADVYDALTAKRPYKEPWPKEKVLKILRQDSENGKFDIKLVEILEKIAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG17</accession>
    <name>B7IG17_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">MutL protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_543</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100607.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_543"/>
    <dbReference type="EnsemblBacteria" id="ACJ75031">
        <property type="protein sequence ID" value="ACJ75031"/>
        <property type="gene ID" value="THA_543"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_543"/>
    <dbReference type="eggNOG" id="COG0849">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046680_0_0_0"/>
    <dbReference type="OMA" id="LGMRYSA"/>
    <dbReference type="OrthoDB" id="595291at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006230">
        <property type="entry name" value="MutL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13941">
        <property type="entry name" value="MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004729">
        <property type="entry name" value="MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01319">
        <property type="entry name" value="glmL_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="455" mass="50217" checksum="E1C5AE618A7E5B04" modified="2009-02-10" version="1">MKVDLVFAEIGSTTTVVTAFHNLDGKVKILAQGEHWTTVNEGDVTIGIERAIENLKEKLGVSNFSWDKFAASSSAAGGLKMTVHGLVYDMTVRAAKEAALGAGAVIKYITAGKMDEFHLHKIKQISPKLILLAGGVDYGEKETVIHNAKLLSKLELDVPIIYAGNIAAAEQVEFILKNSGKTVFVTENVYPKIDQLNVEPTRNIIKEVFAKHITKAPGMEKIYDVVDYEIHTTPGAVMKATQLLSEIYGDVLTVDIGGATTDVDSVTEGSNEIQEITISPEPVAKRTVEGDLGLFVNAHNVIDLIGKENLKHEFENLDELIERISPYPKTDQDEYFISKLALYCFQQGIRRHVGKKKHIYTALGRKLIAEGKDLTAVRYLFGTGGFLSRSKYAKDVLKTVNNLSKLHPMELLPQNEVKIFRDKYYIFAAIGVIASEIDKEIAKKILLEDLEELGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG40</accession>
    <name>B7IG40_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP synthase subunit b</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP synthase F(0) sector subunit b</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">ATPase subunit I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">F-type ATPase subunit b</fullName>
            <shortName evidence="11">F-ATPase subunit b</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 14" type="primary">atpF</name>
        <name evidence="14" type="ordered locus">THA_567</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0).</text>
    </comment>
    <comment type="function">
        <text evidence="10 11">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell inner membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the ATPase B chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579652.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_567"/>
    <dbReference type="EnsemblBacteria" id="ACJ75054">
        <property type="protein sequence ID" value="ACJ75054"/>
        <property type="gene ID" value="THA_567"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_567"/>
    <dbReference type="eggNOG" id="COG0711">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079215_4_5_0"/>
    <dbReference type="OMA" id="FAWKPIL"/>
    <dbReference type="OrthoDB" id="1999641at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01398">
        <property type="entry name" value="ATP_synth_b_bprime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028987">
        <property type="entry name" value="ATP_synth_B-like_membr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002146">
        <property type="entry name" value="ATP_synth_b/b'su_bac/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005864">
        <property type="entry name" value="ATP_synth_F0_bsu_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00430">
        <property type="entry name" value="ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81573">
        <property type="entry name" value="SSF81573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01144">
        <property type="entry name" value="ATP_synt_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 11 12" id="KW-0138">CF(0)</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 11 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="33"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ75054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="161" mass="18561" checksum="FDCF58AD285AEC5F" modified="2009-02-10" version="1">MDMFEINLTSVVQLMSFLLLLYFLKKFLYDKYFEVMDARKEKIEGEIAKAEQLRKEAEELKNEATEELRKIRSEADSIIKKAKDEAENIISDAKKKAEDEASKIIAAAKDEIERQRAEMIKEVEQRVGEIAVALAMKVLKGTLDEKAKREYLVKVLKGSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG46</accession>
    <name>B7IG46_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100555.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_573"/>
    <dbReference type="EnsemblBacteria" id="ACJ75060">
        <property type="protein sequence ID" value="ACJ75060"/>
        <property type="gene ID" value="THA_573"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_573"/>
    <dbReference type="eggNOG" id="COG1307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048251_0_1_0"/>
    <dbReference type="OMA" id="TELYFTI"/>
    <dbReference type="OrthoDB" id="1288126at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003797">
        <property type="entry name" value="DegV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043168">
        <property type="entry name" value="DegV_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02645">
        <property type="entry name" value="DegV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00762">
        <property type="entry name" value="DegV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51482">
        <property type="entry name" value="DEGV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="272" mass="31124" checksum="51006A22AE5BD8E2" modified="2009-02-10" version="1">MIAFIADSSFDMPNVKTKYPIFFTPLRVIIDGKEFVDKVNLTVDQFYDMLKDAEDFSTSLPNPKETEELIKKLYDEYDKIYMLSVSSKLSGTFNMFQMVSQNFKDKVKVLDLKTASIESYAIFRKLVEYAEKDIELTQDIVDKIRKNLKMYFAVMDLKYLEKGGRIGKAKALLGKMLKIKPILSVDETGAVESIAMERKLSSLVNKLIELGESFLKEHKISDPYFLAAYGSKEYKKYIDRLMQHFNIKDITQISAAVGIHTGPEVFGFVVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IG78</accession>
    <name>B7IG78_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tnp_DDE_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104389.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75092">
        <property type="protein sequence ID" value="ACJ75092"/>
        <property type="gene ID" value="THA_606"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_606"/>
    <dbReference type="HOGENOM" id="CLU_2144673_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13586">
        <property type="entry name" value="DDE_Tnp_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tnp_DDE_dom" evidence="1">
        <location>
            <begin position="10"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="112" mass="13864" checksum="945CE8A1CE7689F1" modified="2009-02-10" version="1">MLEDIEGLILLADRGYWQWEFIEKMKEKMKLYVRPRRRKGKEFMEREINRLIYSKRWEIEWYIERLKQRIKMRGMNEKTLKAQITYMLLGQQLTMLINTLSAHPKMIRLIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGA6</accession>
    <name>B7IGA6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rod shape-determining protein RodA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_642</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579697.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_642"/>
    <dbReference type="EnsemblBacteria" id="ACJ75120">
        <property type="protein sequence ID" value="ACJ75120"/>
        <property type="gene ID" value="THA_642"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_642"/>
    <dbReference type="eggNOG" id="COG0772">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029243_2_1_0"/>
    <dbReference type="OMA" id="IFCTVGE"/>
    <dbReference type="OrthoDB" id="1133883at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018365">
        <property type="entry name" value="Cell_cycle_FtsW-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001182">
        <property type="entry name" value="FtsW/RodA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011923">
        <property type="entry name" value="RodA/MrdB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474">
        <property type="entry name" value="PTHR30474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474:SF1">
        <property type="entry name" value="PTHR30474:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01098">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00428">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="355" mass="39992" checksum="BED32AD4E0DCA02C" modified="2009-02-10" version="1">MKENKKFDFIILISVIFLIVFGLLNLYSVSKYLVVKQFFWDLLAIGAAIFVYFLKENLIKKLVIPLYIISVVLLAAVLIFGTRVYGSIRWFRFFGLSFQPSELSKLALILMLSIIFVKKDLRSLFFSILVLSVPVFLILREPDLGMSVLHIFVWFTMLLFSGVSFKYILPIVGSGIGAIPIIYFFFLKDYQRARILSFLNPEKYAQGAAYNVIMSKNAVGSGGLLGRGYLISPAVNGNYVPKMETDFIFSAIGEQFGFLGSILVLAAYLVIIIRVFSKMKDFKDNFWRLVSVGILSAFVFHVFENIGMNIGIMPVTGIPLPFLSYGGTSTFIFGIMIGFLFKSLALVDKSRKVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGA7</accession>
    <name>B7IGA7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_643</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104479.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_643"/>
    <dbReference type="EnsemblBacteria" id="ACJ75121">
        <property type="protein sequence ID" value="ACJ75121"/>
        <property type="gene ID" value="THA_643"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_643"/>
    <dbReference type="eggNOG" id="ENOG5031FJ8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112863_0_0_0"/>
    <dbReference type="OMA" id="PYKFYFF"/>
    <dbReference type="OrthoDB" id="1524212at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="211" mass="24813" checksum="175A531DBDEB26B0" modified="2009-02-10" version="1">MIHKRRRERIWPYVLVFVVIILFIITVFFAFGNFFRYRKILDEYNSLKKFVENKLNGYEVKLKDLESKFGPNSVLDKYILSVNYLKNFGADLEYILNNIEDEPGTGYFMVFVVGNEPSWVTVKDDKKTYFSREIYPGLSKYKFYYFKSPKIMTDYDIIVPPDADIVVGKAGKVYLLFYGVGSSFHPTKIVHIEDMKVDNLMKTFSLYVPGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGB4</accession>
    <name>TSAD_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N6-L-threonylcarbamoyladenine synthase</fullName>
            <shortName evidence="1">t(6)A synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tsaD</name>
        <name type="synonym">gcp</name>
        <name type="ordered locus">THA_650</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579701.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IGB4"/>
    <dbReference type="SMR" id="B7IGB4"/>
    <dbReference type="STRING" id="484019.THA_650"/>
    <dbReference type="EnsemblBacteria" id="ACJ75128">
        <property type="protein sequence ID" value="ACJ75128"/>
        <property type="gene ID" value="THA_650"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_650"/>
    <dbReference type="eggNOG" id="COG0533">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_0_2_0"/>
    <dbReference type="OMA" id="HLEGHIY"/>
    <dbReference type="OrthoDB" id="1257362at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017860">
        <property type="entry name" value="Peptidase_M22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="PTHR11735:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03723">
        <property type="entry name" value="T6A_TsaD_YgjD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01016">
        <property type="entry name" value="GLYCOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000146034" description="tRNA N6-adenosine threonylcarbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="133"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01445"/>
        </source>
    </evidence>
    <sequence length="330" mass="35683" checksum="66AD4177E19EC491" modified="2009-02-10" version="1">MIVLGIETSCDETSVAILSDGKILSNVVSSQIDIHKKFGGVVPEIAARHHLSNLPIVFKNAIDMANISIDQIDLISVTYGPGLIGALLVGISFAKGLSLRLGKPLIGVNHIVGHVFANYITYPHLKPPYIVLMVSGGHTEILLVKQDDEIEVLGKTVDDAAGEAFDKVARILGLGYPGGPEIDKLSKNGDENKFNFPRPMMDSKSYNFSFSGLKTAVLYTVQKFDKDNVPKEDIAASFQKAVVEILLKKTFKAAKDLNVNTIVLAGGVAANSYLRKKAQKLSEKQNIKVLIPPLEFCTDNAAMIAMAGYKLYKKGISSDSTLEAVPNLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGD5</accession>
    <name>B7IGD5_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Ribokinase</fullName>
            <shortName evidence="10">RK</shortName>
            <ecNumber evidence="3 10">2.7.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">rbsK</name>
        <name evidence="13" type="ordered locus">THA_674</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + D-ribose = ADP + D-ribose 5-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:47013"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carbohydrate kinase pfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.15" evidence="3 10"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579718.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_674"/>
    <dbReference type="EnsemblBacteria" id="ACJ75149">
        <property type="protein sequence ID" value="ACJ75149"/>
        <property type="gene ID" value="THA_674"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_674"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_2_2_0"/>
    <dbReference type="OMA" id="IPWRDEL"/>
    <dbReference type="OrthoDB" id="1030724at2"/>
    <dbReference type="UniPathway" id="UPA00916">
        <property type="reaction ID" value="UER00889"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004747">
        <property type="term" value="F:ribokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019303">
        <property type="term" value="P:D-ribose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01174">
        <property type="entry name" value="ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01987">
        <property type="entry name" value="Ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011877">
        <property type="entry name" value="D_ribokin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02152">
        <property type="entry name" value="D_ribokin_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="12">
        <location>
            <begin position="2"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="37"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="218"/>
            <end position="223"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="249"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ75149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="302" mass="33564" checksum="2BD1C6ED0EE6836F" modified="2009-02-10" version="1">MIAVVGSSNMDVVLTVERFTLPGETQKAQKLEFFPGGKGSNQAVSIAKLSKKNSNVYFLTCIGNDSYGRILKGQYDKLNIEGYVVVDDNNGLAFIEVTKKGENRIVIYPGANRNLTKEIIKKHEEKLLEADYILLQNEIPFESTLYSARIFSENGKIVIFDPAPVSGIEKEIFQFVDFLTPNEEEIKLLTEKFFGEFVSYEDSYFKLKSLGVKKLIVKLGSKGVIYFEGDKRIEIPSFKVKAVDTTAAGDVFNGAFVVALHENLDIKKSLEFASAAAALSVTKKGAQNSIPSRKEVENFLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGF0</accession>
    <name>B7IGF0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_693</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579732.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_693"/>
    <dbReference type="EnsemblBacteria" id="ACJ75164">
        <property type="protein sequence ID" value="ACJ75164"/>
        <property type="gene ID" value="THA_693"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_693"/>
    <dbReference type="eggNOG" id="ENOG5031XAC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1703140_0_0_0"/>
    <dbReference type="OMA" id="CPFEDGI"/>
    <dbReference type="OrthoDB" id="1786129at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="149" mass="17107" checksum="3A2E37337F292281" modified="2009-02-10" version="1">MKMSPEMIKAQENMKPGVITAEGFLGEDDRNLVDIILQDEQEMAALKLDFDKVAQKLKKLLEVGEKGLGEPITYENFEIFVYEARGFLACPFEDGIFRKKVAVVKNLKNNESIMYSDLSIHLLEKHHFLQGKGSKFRLEPEKIKKVLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGI3</accession>
    <name>B7IGI3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase, short-chain specific</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_730</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579757.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
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        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
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        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
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        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF56645"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="8">
        <location>
            <begin position="8"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="7">
        <location>
            <begin position="122"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="229"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ75197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="379" mass="41846" checksum="19221876406E707D" modified="2009-02-10" version="1">MEFQLTKLQKWIKKAAREITEKEFKNLAAKVDEEGYFPIENVEKLRKYGFLGMNIPREYGGAGADDLSYVIAVEEISRYCATTGVILSAHNSLACWPIYYYGTDYQKEKFLKPLATGEKLGAFALTEPNAGSDASNQSTYAVKDGNNWIINGTKIFITNGGVADIYIIFASTNKELGAKGISAFIVERDTPGFSIGKIEEKMGIRGSATAELILDNVVVPGENLLGKEGQGFKIALKTLDGGRIGIAAQALGIAQGAFDETVKYIKQREQFGRPIAKFQSVQFMIAEMKTKIEAARCLVYNAALKKMKSSDYSLEAAMAKYYASDVAMEVTRMAVQLHGGYGYTKEYPVERMMRDAKITEIYEGTTEVQKIVISSNILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGN1</accession>
    <name>B7IGN1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ferredoxin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_783</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100350.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_783"/>
    <dbReference type="EnsemblBacteria" id="ACJ75245">
        <property type="protein sequence ID" value="ACJ75245"/>
        <property type="gene ID" value="THA_783"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_783"/>
    <dbReference type="eggNOG" id="COG2221">
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    </dbReference>
    <dbReference type="eggNOG" id="COG4624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027268_0_0_0"/>
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    <dbReference type="OrthoDB" id="123059at2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009016">
        <property type="entry name" value="Fe_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fer4_10"/>
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    </dbReference>
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        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF53920"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="2"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="33"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S" evidence="6">
        <location>
            <begin position="332"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="534" mass="61622" checksum="C3E1A968808FE8C3" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGN7</accession>
    <name>B7IGN7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_789</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75251">
        <property type="protein sequence ID" value="ACJ75251"/>
        <property type="gene ID" value="THA_789"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_789"/>
    <dbReference type="HOGENOM" id="CLU_3085724_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="52" mass="5939" checksum="E912739722EA4566" modified="2009-02-10" version="1">MLFEKCIVIFLSVSLSGVSPMGGWGDGMVWCPCWLFHFFTFFQEGVLLLKHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGR2</accession>
    <name>B7IGR2_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Na(+)-translocating NADH-quinone reductase subunit b (Na(+)-translocating nqr subunit b) (Na(+)-nqr subunit b)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_814</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100410.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_814"/>
    <dbReference type="EnsemblBacteria" id="ACJ75276">
        <property type="protein sequence ID" value="ACJ75276"/>
        <property type="gene ID" value="THA_814"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_814"/>
    <dbReference type="eggNOG" id="COG4658">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042020_1_0_0"/>
    <dbReference type="OMA" id="FCIPPEA"/>
    <dbReference type="OrthoDB" id="1654433at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004338">
        <property type="entry name" value="NqrB/RnfD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30578">
        <property type="entry name" value="PTHR30578"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03116">
        <property type="entry name" value="NQR2_RnfD_RnfE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ75276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="269" mass="30011" checksum="AB393E3D493A12E6" modified="2009-02-10" version="1">MALFQKQVMMRRVLYALLPIYIYSFLFYGLRLIWLSIFVFSFGILAEYIFEKRKNKKVSEAVLVTCMLYTLSLPPLTPWWIAVIGIIFGVVFAKEVYGGFGRNVFNPAITGRLFVYIAFPSFMTKGWMLPKLGSLGLDAVTSATPLSLFDSGKLTNLKDLFFGFRAGSFGEGAIFLIFIGAIYLIITKTASWKIIVSTILSAAFLTFMFDLFIPSAPPTLFALLSGSLLFVSVFIATDPITAPKNSKAQYIYGIIIGVSIVLIRTFYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGS4</accession>
    <name>B7IGS4_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Replicative DNA helicase</fullName>
            <ecNumber evidence="3 12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">dnaB1</name>
        <name evidence="14" type="ordered locus">THA_826</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the helicase family. DnaB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 12"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579819.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_826"/>
    <dbReference type="EnsemblBacteria" id="ACJ75288">
        <property type="protein sequence ID" value="ACJ75288"/>
        <property type="gene ID" value="THA_826"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_826"/>
    <dbReference type="eggNOG" id="COG0305">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005373_0_0_0"/>
    <dbReference type="OMA" id="IEFHARI"/>
    <dbReference type="OrthoDB" id="1709134at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00984">
        <property type="entry name" value="DnaB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036185">
        <property type="entry name" value="DNA_heli_DnaB-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007692">
        <property type="entry name" value="DNA_helicase_DnaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007694">
        <property type="entry name" value="DNA_helicase_DnaB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007693">
        <property type="entry name" value="DNA_helicase_DnaB-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016136">
        <property type="entry name" value="DNA_helicase_N/primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00772">
        <property type="entry name" value="DnaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03796">
        <property type="entry name" value="DnaB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48024">
        <property type="entry name" value="SSF48024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00665">
        <property type="entry name" value="DnaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51199">
        <property type="entry name" value="SF4_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 12 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="8 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 12" id="KW-0639">Primosome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SF4 helicase" evidence="13">
        <location>
            <begin position="177"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACJ75288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="447" mass="50739" checksum="F7318C457ADABA7E" modified="2009-02-10" version="1">MRTPLHNIEAEQAVIGSILIDPEAIENVISIVRSEDFYDNRHVEIFKAIEELYDENIPIDVISICDRLKTKGKLDMVGGELYVAQLADGVPTSAHAETYAQIVRDKSILRSLISAASRVVEEAMSDNDVDEILDNAERVIFEIAESKTSKTYQPMNTILHEVFENLESLRDKMKKGTSSLITGIPTGYRLLDELTSGFHKSDLIIVAARPSVGKTAFALNIARNMAIKADVPVGIFSLEMSREQLAQRLLGMEALIELQKIRRGHLTDEEWQKLLQATGKLYKANIIVDDEANLDPRSLRAKARRMKKEYGVEAIFIDYLQLMSIRSFRENRQQEISEISRSLKLLARELDVSIIALSQLSRAVEQREDKRPRLSDLRESGSIEQDADMVLFLYREEYYKKEKTVEPHETEIIIGKQRNGPIGTIKLIFDPHFTAFFDVDVYHGGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGW0</accession>
    <name>B7IGW0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Succinoglycan biosynthesis protein ExoA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_864</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579844.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_864"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75324">
        <property type="protein sequence ID" value="ACJ75324"/>
        <property type="gene ID" value="THA_864"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_864"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025996_19_0_0"/>
    <dbReference type="OMA" id="YGIYKRE"/>
    <dbReference type="OrthoDB" id="1445278at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="324" mass="37277" checksum="446A13D245C30C75" modified="2009-02-10" version="1">MKKVTIVIPTRNEEKYIEKCINSILENDYPEKEIIVVDGMSSDRTREILKEYKDIKIIDNPEKITPIALNIGIENATGDYIMIAGAHSTYSKNYISECIKRLDEGKCEIAGGKVITKPGNDTKKAIAISRVLSHPFGIGGARYRINPQKEMYVDTVAYGIYKREVFEKVGTFNPNLVRNQDIEMNLRIKNAGMRILLVPNAESYYYARDNFKSLFKNNFQNGLWVILSTKYAKRAFSLRHLVPLFFVLFLLSILFVYKISFLKSVYFSILALYLFLNIFFSLKIALKEKSLKVFFYSFISFLILHISYGLGSFVGIFKRLFSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH17</accession>
    <name>B7IH17_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_922</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75381">
        <property type="protein sequence ID" value="ACJ75381"/>
        <property type="gene ID" value="THA_922"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_922"/>
    <dbReference type="HOGENOM" id="CLU_3349760_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="37" mass="4635" checksum="9FF35EFB10C56829" modified="2009-02-10" version="1">MRLKAELKRIKIFSSYFPPSFFFFGKYYNISKKFFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH50</accession>
    <name>B7IH50_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579900.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_955"/>
    <dbReference type="EnsemblBacteria" id="ACJ75414">
        <property type="protein sequence ID" value="ACJ75414"/>
        <property type="gene ID" value="THA_955"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_955"/>
    <dbReference type="eggNOG" id="COG1161">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017878_0_2_0"/>
    <dbReference type="OMA" id="HYNEVQD"/>
    <dbReference type="OrthoDB" id="942962at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019988">
        <property type="entry name" value="GTP-bd_ribosome_bgen_YqeH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03597">
        <property type="entry name" value="GTPase_YqeH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="154"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="358" mass="41061" checksum="A29BB160FEEAB72D" modified="2009-02-10" version="1">MKCRGCGAELQYTDPKKPGYIPKEILENEPDPLCQRCYRIIHYGKLTVPVQEEIFLHQIDKVIKNFEKVLYVIDITDFEGTYRSEIMEKLQGKDVYFAITKFDLLPRSLSSIEAQEWLKKRLNTTSEKIFLTSSKNGFGLKKLEKFFKDSKKDILIVGVTNVGKSSLLSAFTKEHPTISPFPGTTLGIVKRKVFNTYIYDTPGILTNDRVIDLLSPECQAKILKTNNLTRKTFKIDQSRAILVSAFCKIEVEFDGDKKPIFQIFAPEKVTFHETKSERADQLIHQRAGDLLQPPCKKTDMSKYNFKTERILVDQKQEISINGLCFINIKRGPFVAKITVPENINVTLRDRLLKPKRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH55</accession>
    <name>B7IH55_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Flagellar biosynthetic protein FlhB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">flhB</name>
        <name evidence="13" type="ordered locus">THA_960</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for formation of the rod structure in the basal body of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the type III secretion exporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_960"/>
    <dbReference type="MEROPS" id="N06.A01"/>
    <dbReference type="EnsemblBacteria" id="ACJ75419">
        <property type="protein sequence ID" value="ACJ75419"/>
        <property type="gene ID" value="THA_960"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_960"/>
    <dbReference type="eggNOG" id="COG1377">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041013_1_2_0"/>
    <dbReference type="OMA" id="DEYKQQE"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006136">
        <property type="entry name" value="FlhB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006135">
        <property type="entry name" value="T3SS_substrate_exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029025">
        <property type="entry name" value="T3SS_substrate_exporter_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30531">
        <property type="entry name" value="PTHR30531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01312">
        <property type="entry name" value="Bac_export_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00950">
        <property type="entry name" value="TYPE3IMSPROT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160544">
        <property type="entry name" value="SSF160544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00328">
        <property type="entry name" value="flhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="11 12" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="5 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="13" id="KW-0282">Flagellum</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ACJ75419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="312" mass="35524" checksum="AF3406728C0EE1CF" modified="2009-02-10" version="1">MLGFAFLGRGIIIGLQSAVAFFSKLPSYDSLTIQDLGIYTIEAFKGVIIDLIIFVGLVMITGIITGSLQTKFLITFKSIKLDFSKINPISGLKRMFSLRSLFELLKSILKLVIVGYVGYLVVKSNWNKLLLATDTSVSEGALLIWDITFELLIKCGIALFIVSIADYFFSRYEYEKNIRMTKQEVKEEFKEVEGNPEIKRKQRQIMMQYSMHRMMQEVPEATVVVTNPTHFAVAIKYDSDEMDVPIVVAKGADKLAQKIKEIARKNNVPILRNPPLARELYFSVEIGDEIPQKLYRAVAEVLAYVYTLKERY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH70</accession>
    <name>B7IH70_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">THA_975</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100944.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_975"/>
    <dbReference type="EnsemblBacteria" id="ACJ75434">
        <property type="protein sequence ID" value="ACJ75434"/>
        <property type="gene ID" value="THA_975"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_975"/>
    <dbReference type="eggNOG" id="COG0489">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_0"/>
    <dbReference type="OMA" id="NMAYFTP"/>
    <dbReference type="OrthoDB" id="1413173at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="PTHR23264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="30"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="270" mass="29478" checksum="D1DBFF862EA90C0F" modified="2009-02-10" version="1">MQPQFNLNNENTKIKEKMKNVKHKIAVLSGKGGVGKTTVAVNLATALAESGYKVGLLDLDMHGPNIVRMLGEKNPSVDGEEIVPAEILPNLKALSIGMLVESGKAVIWRGPLKHSAIKQFLGDTKWGELDFLIFDLPPGTGDEALSLFQTLDDLDGVVMVTTPQKVALDDVRRAIDFVHSMNKKLIGIVENMSFVRCPKCGEKIEIFGSGGGKILAEEYNVELLGQIPLDPKAAKLADEGKPVTLYMRESEVEEEFRKIAEKVAKAVEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH83</accession>
    <name>B7IH83_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integrase, catalytic region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_988</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100976.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_988"/>
    <dbReference type="EnsemblBacteria" id="ACJ75447">
        <property type="protein sequence ID" value="ACJ75447"/>
        <property type="gene ID" value="THA_988"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_988"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_31_2_0"/>
    <dbReference type="OMA" id="WENTAIK"/>
    <dbReference type="OrthoDB" id="1637053at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025948">
        <property type="entry name" value="HTH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13276">
        <property type="entry name" value="HTH_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="154"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="341" mass="40419" checksum="238DFF9D58A7ECBE" modified="2009-02-10" version="1">MRLTFNSQSEKEVTFIGDFLLIFKYRNFGVSTTFLLGYYNVSSSSFHYNTRLFFVVKRNRKSSFKGYSFTNDGKKVPDDYIKQLLIELLSVDNPSNPEFFYKTLGSKKLSAIFRQKFNIIINHKKIHRLRKELNLVRYYHRHPKHPRKRPKNHDITRPNQYWESDIKFIPTKYDGYIPILSIIDAFDRSIVGVYIGNSIKAKDFISTVRKSIEFRGTIPNNLIIRTDNGPQFKAKITAAFMEELNIIHEFGYKNNPNSQAYIESFHSSVQHEFVESNEFDYIDDVYNYYISYIYFYNNLRPHGSLNYFTPEFVFNLFSNTNNESNEIKSIKFNDFIVCVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH84</accession>
    <name>B7IH84_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_989</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100978.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_989"/>
    <dbReference type="EnsemblBacteria" id="ACJ75448">
        <property type="protein sequence ID" value="ACJ75448"/>
        <property type="gene ID" value="THA_989"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_989"/>
    <dbReference type="HOGENOM" id="CLU_218917_1_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="Methyl-accepting transducer" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="37" mass="3987" checksum="43536E57BBD4C57E" modified="2009-02-10" version="1">MNEQAKNIGQIFETINSIAEQTNLLALNADIEVAIVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH86</accession>
    <name>EFTS_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor Ts</fullName>
            <shortName evidence="1">EF-Ts</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tsf</name>
        <name type="ordered locus">THA_991</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EF-Ts family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579917.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IH86"/>
    <dbReference type="SMR" id="B7IH86"/>
    <dbReference type="STRING" id="484019.THA_991"/>
    <dbReference type="EnsemblBacteria" id="ACJ75450">
        <property type="protein sequence ID" value="ACJ75450"/>
        <property type="gene ID" value="THA_991"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_991"/>
    <dbReference type="eggNOG" id="COG0264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047155_1_1_0"/>
    <dbReference type="OMA" id="DAGMMDC"/>
    <dbReference type="OrthoDB" id="1405357at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00050">
        <property type="entry name" value="EF_Ts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036402">
        <property type="entry name" value="EF-Ts_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001816">
        <property type="entry name" value="Transl_elong_EFTs/EF1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014039">
        <property type="entry name" value="Transl_elong_EFTs/EF1B_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018101">
        <property type="entry name" value="Transl_elong_Ts_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11741">
        <property type="entry name" value="PTHR11741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00889">
        <property type="entry name" value="EF_TS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="SSF46934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54713">
        <property type="entry name" value="SSF54713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00116">
        <property type="entry name" value="tsf"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01126">
        <property type="entry name" value="EF_TS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01127">
        <property type="entry name" value="EF_TS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000189894" description="Elongation factor Ts">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Involved in Mg(2+) ion dislocation from EF-Tu" evidence="1">
        <location>
            <begin position="80"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00050"/>
        </source>
    </evidence>
    <sequence length="196" mass="22454" checksum="C347D153FE3BA474" modified="2009-02-10" version="1">MIDAKLVKELRDRTGAGMMDCKKALEEANGDMEQAIEILRKKGIAKAAKKASRETGDGIIAAYVHFNKRIGVLVELNCETDFVARTEDFQELGNKIAMHIAAMSPRWVRREDVPQEVIEKEKEIYRDQLKDSGKPEHVIEKIVEGKLNKFFEENCLYEQKFAFDEDKTVEELIKESIAKIGENIRVSRFVKYTVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>B7IH95</accession>
    <name>B7IH95_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_1000</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100997.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1000"/>
    <dbReference type="EnsemblBacteria" id="ACJ75459">
        <property type="protein sequence ID" value="ACJ75459"/>
        <property type="gene ID" value="THA_1000"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1000"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_0"/>
    <dbReference type="OMA" id="LVDMQGY"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="4">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="205" mass="24075" checksum="0F2080C434E95D13" modified="2009-02-10" version="1">MNFSNEHSNKREGQIVWSDEKNFIKALKKGEEWAYRRLYREYAPKIGAFARSYFGTDDVDDVIQEVMLRIYKGIKKFKGDSSLSTWIYRITVNVCNTLYEKYKKKNEKTFSVENNNSEDDVEIDIPDKETDVQKEVTQEILYEKILKALEKLSEKERVLIRMRDIDGLSYSEIAEILGIPEGTVKSRLHNAREKFKKILEEEGIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHB4</accession>
    <name>B7IHB4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1020</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579938.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1020"/>
    <dbReference type="EnsemblBacteria" id="ACJ75478">
        <property type="protein sequence ID" value="ACJ75478"/>
        <property type="gene ID" value="THA_1020"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1020"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_0_0"/>
    <dbReference type="OMA" id="MQEDYMR"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="97"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="335" mass="37793" checksum="B0680C4D3394DE86" modified="2009-02-10" version="1">MSLKSYIITRIILALPMILILLIMIFFILRIIPGDPVLAILGGKAPLEVIEAKRHELGLDKPIVVQFFNYLKDVTKGDLGVSTLTNRPVWSEIKDRFPATLELTIFSFIFALIIGIFWGAEAAWRKDKSLDISARVYSIFIYAVPVFWLGLMLQLLFGIYLRWLPVGGRLSPRVSLDIKTGLLLLDSILNWNWEAFWDVLKHLLLPGITLGLVISSIFLRMVRNNTVLMLSKDFVKAAKARGLKPKVILYGHAVRNAFVPIFTIMGMQFALLLAGAVLTETTFSWPGIGSYLVLKIKYRDFPAIQGTIIFFAIFVVIISIVIDIVNALVDPRVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHF0</accession>
    <name>B7IHF0_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XylR-related</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1058"/>
    <dbReference type="EnsemblBacteria" id="ACJ75514">
        <property type="protein sequence ID" value="ACJ75514"/>
        <property type="gene ID" value="THA_1058"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1058"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_13_5_0"/>
    <dbReference type="OMA" id="AWGELAF"/>
    <dbReference type="OrthoDB" id="1130699at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="342" mass="38671" checksum="577DFCD605E14518" modified="2009-02-10" version="1">MITDSLKKIISYSWKNKVVTAKNISFDLGLEKSTVSRNISKLRGKNILIKTDELSPSNLGGRKTIVYSFNEKLGHILGISVEQGGIEYVKTDLFSNVLEKKKIVEKINEKNIAFILREIIKNNEDVLGVGISIPGIIENNTVVFSEALSLKNFDLEKTLLLDIPIFVEKDSICGATRYSLESKNIVYFQLSVPYYVNEPVGFGVGIVIDGKPYYGHSNFSGEYKLNKSIYNKKISYEEFLKKSVSLDEFFVDISKKIGQISSILDPEIVVIGGNITLISGIENLRDFIVEQVYMYEHRKMEIVIEDAREFVNAEGAAINVLKNMFLKEEGIEYFCKKVILNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHF3</accession>
    <name>B7IHF3_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1061</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1061"/>
    <dbReference type="EnsemblBacteria" id="ACJ75517">
        <property type="protein sequence ID" value="ACJ75517"/>
        <property type="gene ID" value="THA_1061"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1061"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_0"/>
    <dbReference type="OMA" id="FGNNQFF"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="72"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ75517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="295" mass="33391" checksum="69DA71C504D45476" modified="2009-02-10" version="1">MVILKRKTRYNVETFLLLLPFLILFAVFGVFPIAYSFFMSFTDYSALSPNFNFVGLKNYIRMFHDEVFLTALKNTVLFVVGTIPFTTVISLLLAVLINSKFLPLKDLFKAGFFLPTVVSMVVISTTWMYLYSADGFFNKMLEFFGMNPIPTSWLAHTKTALLSIMIMDVWAAIGYYTILFLAGLQSIPQQLYEAAAIDGASKSKIFFKITLPLLKPTLYFVIALNTIRSFQIFSEIFTMTGGGPMNSTQTIVHYLYIVGFRNFEMGYASAIAYVLVLIIFLVTLVQGKLLRSESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHH8</accession>
    <name>B7IHH8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein containing ATP-binding domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579991.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1086"/>
    <dbReference type="EnsemblBacteria" id="ACJ75542">
        <property type="protein sequence ID" value="ACJ75542"/>
        <property type="gene ID" value="THA_1086"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1086"/>
    <dbReference type="eggNOG" id="COG2102">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010289_1_0_0"/>
    <dbReference type="OMA" id="DIDLQEH"/>
    <dbReference type="OrthoDB" id="1144452at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="Alpha_ANH_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039123">
        <property type="entry name" value="Diphthamide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthami_syn_2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="211" mass="24198" checksum="1FC5A5F160BBEE54" modified="2009-02-10" version="1">MKVFCSFSGGKDSMLALYYGLKKYKKVDYLFTMLSEDCIHSRAHGLPKSLLEKQAQSIGIRLITKCSSWKEYENNFLSFLDEYVKGGVGIFGDIDLQEHLDWVENVCSKKDVEVFEPLWLKKRKDVVSEFLSLGFKAKIIALKKDLGIEKYLGKDLTFDLADEFEKIGVDACGENGEFHTFVYDGPIFKYPVDFKVKGTVQKERNIVLELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHI9</accession>
    <name>B7IHI9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hypothetical cytosolic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579999.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1097"/>
    <dbReference type="EnsemblBacteria" id="ACJ75553">
        <property type="protein sequence ID" value="ACJ75553"/>
        <property type="gene ID" value="THA_1097"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1097"/>
    <dbReference type="eggNOG" id="COG2122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074757_1_0_0"/>
    <dbReference type="OMA" id="YGVCTSS"/>
    <dbReference type="OrthoDB" id="1979718at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="231" mass="25698" checksum="3B0B57A733CD1E27" modified="2009-02-10" version="1">MKRFYRNFMGNNLFSFSIKYKESDLWIALDKKETGLSVLAYNYLISLRKELEEYATNTFLTSLKPLAFDENAPKVAKDMLKASKKANVGPMAAVAGAFAKHIGQYIKKLVNPKKLLVENGGDLYVYSHEPTTIMILSTKTNLYLDLPPGEFGVATSSRKIGHSKNFGSTDTTTVVCSDPALADAYATAISNKIKNVHDAKNLLSRKFEEIQDIIVTIDKNIIIKAIHNVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHL7</accession>
    <name>B7IHL7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">LicC-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580018.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1128"/>
    <dbReference type="EnsemblBacteria" id="ACJ75581">
        <property type="protein sequence ID" value="ACJ75581"/>
        <property type="gene ID" value="THA_1128"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1128"/>
    <dbReference type="eggNOG" id="COG1213">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_5_0_0"/>
    <dbReference type="OMA" id="AYSLWCA"/>
    <dbReference type="OrthoDB" id="1557977at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="1">
        <location>
            <begin position="3"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="236" mass="28009" checksum="1D40C4CF64C63C5A" modified="2009-02-10" version="1">MKAILLAAGKGTRFSRKISNIPKSLVDIKGKPLILRTIEMLRQNNIDVSVILGYKRELFLEILDEVKVYYNPFYSVTNSIASLWFAKDEFSGKEDIIVANADVYWDREILKTLCNSKEDILMLYDSSRKEEGDYLFYVEDNILKDHGKELLPEKISGEYVGVSIIRKEFQEKFRERLELLISQEKYDLWWENVLYSFIDERNVYVKDIKGLFWGEVDYIEDYYRIVNHLRKLEVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHN2</accession>
    <name>B7IHN2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF218 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1144</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580032.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1144"/>
    <dbReference type="EnsemblBacteria" id="ACJ75596">
        <property type="protein sequence ID" value="ACJ75596"/>
        <property type="gene ID" value="THA_1144"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1144"/>
    <dbReference type="eggNOG" id="COG1434">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053514_1_1_0"/>
    <dbReference type="OMA" id="LDFRHFG"/>
    <dbReference type="OrthoDB" id="1879123at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06259">
        <property type="entry name" value="YdcF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003848">
        <property type="entry name" value="DUF218"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02698">
        <property type="entry name" value="DUF218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="DUF218" evidence="2">
        <location>
            <begin position="77"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="241" mass="27749" checksum="9EB9021097011667" modified="2009-02-10" version="1">MIYLYKFIGSFVQFPGILIAFFSLLTLYYFLKKRQIWRFFLILSIVFYLISSPFFVYFLSKSFYIKEKNIPDSQNSVIVILGGGITFFDGKYEVGNHTLKRLLKGFEIYRKTNAPIIISGGVIGKGISESAIMKDILVTFGVNINDIIVEDKARTTKENAIFVSKLLKGKNFENIILVTSFLHMKRSIMLFEKYSKKNIIPIVCDYPIDFRNSFLDYLPSAEALYTFSQITHEFFGILKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHQ5</accession>
    <name>B7IHQ5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1168"/>
    <dbReference type="EnsemblBacteria" id="ACJ75619">
        <property type="protein sequence ID" value="ACJ75619"/>
        <property type="gene ID" value="THA_1168"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1168"/>
    <dbReference type="HOGENOM" id="CLU_2557168_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="82" mass="9604" checksum="FDE97FF4E113F9B4" modified="2009-02-10" version="1">MYIPGKNDQDRENSENEGLKVDTKHVSEKIRQLQLKLQIEEDPVKREELERQILMLEIAKNALQFGMSFPKYLLGILFDQTA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHU9</accession>
    <name>RL2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplB</name>
        <name type="ordered locus">THA_1218</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580081.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHU9"/>
    <dbReference type="SMR" id="B7IHU9"/>
    <dbReference type="STRING" id="484019.THA_1218"/>
    <dbReference type="EnsemblBacteria" id="ACJ75663">
        <property type="protein sequence ID" value="ACJ75663"/>
        <property type="gene ID" value="THA_1218"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1218"/>
    <dbReference type="eggNOG" id="COG0090">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036235_2_1_0"/>
    <dbReference type="OMA" id="SCIELRP"/>
    <dbReference type="OrthoDB" id="961486at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_B">
        <property type="entry name" value="Ribosomal_L2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Rbsml_prot_L2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005880">
        <property type="entry name" value="Ribosomal_L2_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_L2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_L2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_L2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="PTHR13691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01171">
        <property type="entry name" value="rplB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000141628" description="50S ribosomal protein L2">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="274" mass="30195" checksum="7A63D63F5A8AE93C" modified="2009-02-10" version="1">MGLRRFKPTSPARRQMIIPDFSEITKKEPEKSLIAPLKKTGGRNSYGRVTVRFRGGGHKRRYRIIDFKRDKVGIPARVVSIEYDPNRTARIALLVYADGEKRYILAPQGLNVGDTVLNGPDAEIKPGNALPLENIPVGTIVHNVEFIPGKGGQIARSAGTSCQLMAKEGKYALLRMPSGELRKVPVKCYATIGVVGNEDHKNEVDGKAGRVRWKGRKPHVRGVAMNPVDHPHGGGEGRGKGHHPQSPWGQLAKGYKTRRGKKASDKLIVRRRNG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHV1</accession>
    <name>RL22_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplV</name>
        <name type="ordered locus">THA_1220</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101446.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHV1"/>
    <dbReference type="SMR" id="B7IHV1"/>
    <dbReference type="STRING" id="484019.THA_1220"/>
    <dbReference type="EnsemblBacteria" id="ACJ75665">
        <property type="protein sequence ID" value="ACJ75665"/>
        <property type="gene ID" value="THA_1220"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1220"/>
    <dbReference type="eggNOG" id="COG0091">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083987_3_1_0"/>
    <dbReference type="OMA" id="KRIQPRA"/>
    <dbReference type="OrthoDB" id="1666043at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_B">
        <property type="entry name" value="Ribosomal_L22_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_L22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_L22/L17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_L22/L17_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005727">
        <property type="entry name" value="Ribosomal_L22_bac/chlpt-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="PTHR13501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="SSF54843"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01044">
        <property type="entry name" value="rplV_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000142320" description="50S ribosomal protein L22">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <sequence length="148" mass="16923" checksum="38E9EAD8820E90CA" modified="2009-02-10" version="1">MQVLIKRNGMKRSKFHRERKEKLATMPVYEARAVAKFVRISPRKARSVANSIKGKNVSEAFTILEFSPKKAARLIKNVLKSAVANAVNNHGLNEENLYVYTCYVNDGPRMKRIWPRGRGSADIIQKRMSHITVIVRDKEAEKAAKEEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHW2</accession>
    <name>RL18_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplR</name>
        <name type="ordered locus">THA_1231</name>
    </gene>
    <organism>
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>TCF52B</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101467.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B7IHW2"/>
    <dbReference type="SMR" id="B7IHW2"/>
    <dbReference type="STRING" id="484019.THA_1231"/>
    <dbReference type="EnsemblBacteria" id="ACJ75676">
        <property type="protein sequence ID" value="ACJ75676"/>
        <property type="gene ID" value="THA_1231"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1231"/>
    <dbReference type="eggNOG" id="COG0256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098841_0_1_0"/>
    <dbReference type="OMA" id="NKQIYAQ"/>
    <dbReference type="OrthoDB" id="1963491at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_B">
        <property type="entry name" value="Ribosomal_L18_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005484">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004389">
        <property type="entry name" value="Ribosomal_L18_bac-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899">
        <property type="entry name" value="PTHR12899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00861">
        <property type="entry name" value="Ribosomal_L18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00060">
        <property type="entry name" value="L18_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000142728" description="50S ribosomal protein L18">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <sequence length="122" mass="14180" checksum="BB032BF805EB4300" modified="2009-02-10" version="1">MIKKENRNWRRKKRHLSIRKKIHGTADRPRLCVYKSEKHIYAQIIDDDKGHTLVAASTLDKELRETLKKTWNKEAAREVGKLIGKRAIEKGIKKVVFDRGGYRYHGRVAELAEGAREAGLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHY9</accession>
    <name>B7IHY9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cas5h crispr-associated protein Cas5, hmari subtype</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580096.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1258"/>
    <dbReference type="EnsemblBacteria" id="ACJ75703">
        <property type="protein sequence ID" value="ACJ75703"/>
        <property type="gene ID" value="THA_1258"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1258"/>
    <dbReference type="eggNOG" id="COG1688">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090888_1_0_0"/>
    <dbReference type="OMA" id="PWGHFRR"/>
    <dbReference type="OrthoDB" id="1815464at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021124">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013421">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5_HALMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013422">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09704">
        <property type="entry name" value="Cas_Cas5d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02592">
        <property type="entry name" value="cas_Cas5h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02593">
        <property type="entry name" value="CRISPR_cas5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="236" mass="27768" checksum="C7B96BCBFCB02CEC" modified="2009-02-10" version="1">MEFLVFDIKGKFAHFRKFYTNSSSLSYSIPPRTTLEGIIAAILGFERDSYYEILNAEKLNIGLKKVTPTRKIIQTLNYIKAKNPGDVYDPDEHTQIPFEIITSNDKVIYKVYVNHIDQRIMEFLEYRLKNNKFYFIPYFGVASFNVSIEFKGKFQVEPKFSEDFVKVSSVIRRSLISSLKVEEEIILLKEKMPRDFSKERTVIEMEDYIFEESGKAIEVKLNSHYYVINEENIVLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-05-05" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>C0QMU6</accession>
    <name>C0QMU6_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase repeat family IS4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_2020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACN29513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_2020"/>
    <dbReference type="EnsemblBacteria" id="ACN29513">
        <property type="protein sequence ID" value="ACN29513"/>
        <property type="gene ID" value="THA_2020"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_2020"/>
    <dbReference type="HOGENOM" id="CLU_1093873_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="DDE_Tnp_1" evidence="2">
        <location>
            <begin position="101"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACN29513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="254" mass="31143" checksum="7667CA2F0F3C0214" modified="2009-05-05" version="1">MRIIHLKIIIPQKLINKLSSLLFDKTKRMYLRKYPDHIFLLILILMYLTNIQSMRRLYRYLVDGRIRISNLPSRQTIHYRLKRFSRKKMSKKLKKLFGTLLVIDSTEIKDRYTRLHVLYEAKEKVLIDFKIGEYSETEQAELLLEEVENSILLADRGYWVWRFLERVKDRMRLYVRPRGKEGKKFLKSELNRYIYSKRWEIEWFIERMKQRMKLKGMNEKTLKAQITYMLLGMQLTFLIDTLIHHPKLFRFIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>B7ICU8</accession>
    <name>B7ICU8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycogen debranching enzyme GlgX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">glgX</name>
        <name evidence="4" type="ordered locus">THA_1380</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580193.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1380"/>
    <dbReference type="CAZy" id="CBM48">
        <property type="family name" value="Carbohydrate-Binding Module Family 48"/>
    </dbReference>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75825">
        <property type="protein sequence ID" value="ACJ75825"/>
        <property type="gene ID" value="THA_1380"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1380"/>
    <dbReference type="eggNOG" id="COG1523">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011725_1_1_0"/>
    <dbReference type="OMA" id="SEPWDCG"/>
    <dbReference type="OrthoDB" id="99080at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004133">
        <property type="term" value="F:glycogen debranching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02856">
        <property type="entry name" value="E_set_GDE_Isoamylase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044505">
        <property type="entry name" value="GlgX_Isoamylase_N_E_set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011837">
        <property type="entry name" value="Glycogen_debranch_GlgX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02100">
        <property type="entry name" value="glgX_debranch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="3">
        <location>
            <begin position="184"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ACJ75825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="728" mass="84070" checksum="2FABB5EECB1EDC9F" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>B7ID05</accession>
    <name>B7ID05_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580224.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1439"/>
    <dbReference type="EnsemblBacteria" id="ACJ75882">
        <property type="protein sequence ID" value="ACJ75882"/>
        <property type="gene ID" value="THA_1439"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1439"/>
    <dbReference type="eggNOG" id="COG1725">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_10_4_0"/>
    <dbReference type="OMA" id="IFEMFSI"/>
    <dbReference type="OrthoDB" id="1860081at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="128" mass="14588" checksum="5397D5B921CF0B1E" modified="2009-02-10" version="1">MWFTINFSSHKPVYKQIIENIKLQIMNKKLKKGDFLPSIRKMAEMLDVNLNTVSRAYRELVSEGIIEPIRGEGYIVKKDILDNFNEELINELTIIVKKCINAGMKVDDICNIIKSAGGITNATKSRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDF2</accession>
    <name>B7IDF2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">STN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_1591</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580282.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1591"/>
    <dbReference type="EnsemblBacteria" id="ACJ76029">
        <property type="protein sequence ID" value="ACJ76029"/>
        <property type="gene ID" value="THA_1591"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1591"/>
    <dbReference type="eggNOG" id="COG4796">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_640810_0_0_0"/>
    <dbReference type="OMA" id="IIVYCPD"/>
    <dbReference type="OrthoDB" id="2116689at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011662">
        <property type="entry name" value="Secretin/TonB_short_N"/>
    </dbReference>
    <dbReference type="SMART" id="SM00965">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="STN" evidence="5">
        <location>
            <begin position="44"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ76029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="438" mass="50531" checksum="68C43AE5784459DB" modified="2009-02-10" version="1">MKKIIIVILALFFTIHAFSITKDVYFENMDIKDALMQLGSLYNTSIIFPESLNGKITVELYNVSIETALNVILSNFNYTFEKINDIYFIITDQSILYYKSHTYTPKFRSPEELSKLIPFKNYIVGNNIIVYCPDDLWNNYKNLLENIDKDAKNNTIISYAIYYIKNSELKNYNIDTKKLLEALPYTSKFNYILNTFGFFTGNDPKVSVKGSIKLNASVNNSEIIFEISTENDSASAKFSANEGEVRKILEGKFGKFIVITNIKKINYESSSYKIENLKLPLKNSFNLTYFSNQNLLLSASTENLSIFFEKNTFSYLGAKFNPINDFWLGGKINVQKATETSIIFEDKFSINPLYLEFNIDKQLNLEKMELNNILSNLYMKFGIGLKYYIDKNAFIDISAFYDSLEIISGRLKLFHNNFGAVLYIDQNLNYGGGIYINW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDG5</accession>
    <name>B7IDG5_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_1604</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580290.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1604"/>
    <dbReference type="EnsemblBacteria" id="ACJ76042">
        <property type="protein sequence ID" value="ACJ76042"/>
        <property type="gene ID" value="THA_1604"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1604"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_6_0"/>
    <dbReference type="OMA" id="HYLMYEL"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="3"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ76042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="200" mass="23117" checksum="80E602983D9D6337" modified="2009-02-10" version="1">MPHILIIDDERNVRFLIEKFLEGFTYDSLSTVEEALKNIFEKNINYDLIISDLKLPGKSGIELVKALREKAIDTPILIVSAYTKPEILSELFKFERVDFLSKPFTKEELLSKVKELIDEPKESFDRALRMANEFLNSGDLNKAEKLIKKMFSLSPSSPIPHYLMYELLKKRGNLELANKHLNAAKSLDENIEVKKDEENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7IDJ7</accession>
    <name>B7IDJ7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Motility protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">THA_1636</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MotA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580315.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1636"/>
    <dbReference type="EnsemblBacteria" id="ACJ76074">
        <property type="protein sequence ID" value="ACJ76074"/>
        <property type="gene ID" value="THA_1636"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1636"/>
    <dbReference type="eggNOG" id="COG1291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079895_1_0_0"/>
    <dbReference type="OMA" id="EIETHHQ"/>
    <dbReference type="OrthoDB" id="897037at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000540">
        <property type="entry name" value="Flag_MotA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002898">
        <property type="entry name" value="MotA_ExbB_proton_chnl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01618">
        <property type="entry name" value="MotA_ExbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01307">
        <property type="entry name" value="MOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="MotA_ExbB" evidence="9">
        <location>
            <begin position="101"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ76074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="262" mass="27866" checksum="838185409758D383" modified="2009-02-10" version="1">MDIALIGGIGIAFAMVVFGIVSGGGDFAAFINIPSIVIVIGGSIGAAIAANPKDIGFKIVQIALEALREPKTDYVNLLKTLVSLSEKARREGLLSLEENIEQLDDPFMKKGLQLLVDGTEPEVLKSMLEIEVEVASNEMNLKKGLFEAIGAYAPAFGMIGTLIGLIQMLKSLNDPNSLGPSMAVALITTLYGSIMANAMALPIAEKIAKRVISFEMEKTMILEGILSIQAGENPRILEEKLKSFLPDAVKAQYEAATQEAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>B7IEB8</accession>
    <name>B7IEB8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Quinolinate synthase</fullName>
            <ecNumber evidence="3">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nadA</name>
        <name evidence="10" type="ordered locus">THA_1921</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="3"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ76345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580483.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1921"/>
    <dbReference type="EnsemblBacteria" id="ACJ76345">
        <property type="protein sequence ID" value="ACJ76345"/>
        <property type="gene ID" value="THA_1921"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1921"/>
    <dbReference type="eggNOG" id="COG0379">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047382_0_0_0"/>
    <dbReference type="OMA" id="CFCSTMN"/>
    <dbReference type="OrthoDB" id="613347at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="PTHR30573"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="SSF142754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ACJ76345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="303" mass="34391" checksum="6F7D571AA5A724C0" modified="2009-02-10" version="1">MEKIQEKIRKLAKEKGYMILAHNYQIKEIQEIADHLGDSLQLARLATKLNAEKILFLGVDFMAETIKMLNPEKKVIVPVKAATCPMANSLDPEMIIEAKKKYNAPFVIYVNSTMKSKMYADVACTSANAVEIVKKLNSDVVLFGPDKNLAAYVARKTGKKVIPVPGETGYCYVHNYIKIDDVLKLKQKYPDAKVISHPEVPEEIQKVSDYIGSTSQMEKIPGEIEDKKFIIVTEKDMVNKLKNKYKEKEFIPVDTAICNNMKKNTLKNVLKALENEEFEIEIDKEIADKAVLPIKKMFELMEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFA2</accession>
    <name>B7IFA2_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine desulfurase</fullName>
            <ecNumber evidence="4">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">THA_265</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">TCF52B</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="4"/>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579456.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_265"/>
    <dbReference type="EnsemblBacteria" id="ACJ74766">
        <property type="protein sequence ID" value="ACJ74766"/>
        <property type="gene ID" value="THA_265"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_265"/>
    <dbReference type="eggNOG" id="COG0520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_2_5_0"/>
    <dbReference type="OMA" id="HKLCGPT"/>
    <dbReference type="OrthoDB" id="446447at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006534">
        <property type="term" value="P:cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06453">
        <property type="entry name" value="SufS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010970">
        <property type="entry name" value="Cys_dSase_SufS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01979">
        <property type="entry name" value="sufS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="5">
        <location>
            <begin position="23"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ACJ74766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="413" mass="46505" checksum="7DCB816E38838291" modified="2009-02-10" version="1">MLSKNILSDFPALSRTIKGNRIVYLDSAASTLKPKPVIDKLSKFYLENYANVHRAVHTLASESTQMLEFSRKNFANFLNAGEHEIIFTSGTTMSINLIVESLVRSKMLNENDIVLTTLVEHHANFVPWVRLSKLHGFRVEFVEPSLRFGTLAIDDFLKKDINPKVVAVTGHSNVTGQLIDIEKIRSIFPNSILIVDGAQLIPHQRIDVKSLDIDFLCFSVHKMLGPSGIGVLYGKSQYLEALEPFLYGGEMIDKVSVEDVTFNVLPYKFEAGTPNIGGIVGANYALEYLNNIGYDEAKKHVLDLTKYAIERFRNLENVEVYGPLDESHCGILSFNINGIHPHDVAHLLDEKFGVAVRSGHHCAQPLMNVLKSQSRLSQFPNSTCRASFYIYNTYEDIDILIEGIKRIKEWFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFR9</accession>
    <name>B7IFR9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Endoglucanase M</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">THA_442</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">TCF52B</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579582.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_442"/>
    <dbReference type="EnsemblBacteria" id="ACJ74933">
        <property type="protein sequence ID" value="ACJ74933"/>
        <property type="gene ID" value="THA_442"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_442"/>
    <dbReference type="eggNOG" id="COG1363">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047249_0_2_0"/>
    <dbReference type="OMA" id="YTLDNKA"/>
    <dbReference type="OrthoDB" id="900854at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ACJ74933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="334" mass="36612" checksum="03A9F8533D649CE7" modified="2009-02-10" version="1">MKELIKKLTETHSPSGREHQIREVILSELEGFIDGYEVDKLGNLIVWKKGSSDKKVLFDAHMDEIGVVVTNIDDNGFLRIDMVGGVSPYTILQSKLRFGDIIGIVGIEGETGSDMISNIKNLSFDKLYVDIGAKSKEEAEKICPIGTFGTFDGYFVEQGDYYISKSMDDRIGCAVIIELFKKLKNPKNSVYGVFAVQEEVGLVGATVAGYNIDPDVAIAIDVTAAGDTPKGYKRVSMKLGNGACIKVKDNASISDKKIIDTLKDLAEKNKIPYQMEVLIFGGTDARGYQHTKAGIPSATVSIATRYIHTPNEMVHKKDVEATIDLLLKYAEEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>B7IFT8</accession>
    <name>B7IFT8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ74952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104240.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_461"/>
    <dbReference type="EnsemblBacteria" id="ACJ74952">
        <property type="protein sequence ID" value="ACJ74952"/>
        <property type="gene ID" value="THA_461"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_461"/>
    <dbReference type="eggNOG" id="ENOG5033E3U">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1127742_0_0_0"/>
    <dbReference type="OMA" id="VHYLFRV"/>
    <dbReference type="OrthoDB" id="1617223at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ74952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="226" mass="25985" checksum="90E78C4A4C64EE61" modified="2009-02-10" version="1">MRRRRRNTGWIWIVLTLVIIAGIIGAFFWKIYSVKNNEEFNLSYVSQYLFIDKSNEEGFYVVVNGSKRTVNILKIKNHTFDPEKKQEINFSSPILALKILGEMFNVDSNYKYYVVVDTGKIASAAKKLNVNASSFDELFNVLSERGLKIFDFFKLNSILKELRPETTLNAPSIAKLIYSLGNFSVRFKDIPTLTKRPLSITVGDSTFERIYIDTEKIQQLKNEIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGA4</accession>
    <name>B7IGA4_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ssb single-strand binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_638</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_638"/>
    <dbReference type="EnsemblBacteria" id="ACJ75118">
        <property type="protein sequence ID" value="ACJ75118"/>
        <property type="gene ID" value="THA_638"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_638"/>
    <dbReference type="eggNOG" id="COG0629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2738702_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="71" mass="8298" checksum="5740901214246EF2" modified="2009-02-10" version="1">MTKGRLVLVEGKLRINRWQTSDGQNRTTPEIWADQVTFMDKKDDVPIVPEAENEIEYDELFDNNDSDEPPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGF9</accession>
    <name>B7IGF9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase, group 2 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">THA_702</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579739.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_702"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ACJ75173">
        <property type="protein sequence ID" value="ACJ75173"/>
        <property type="gene ID" value="THA_702"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_702"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038143_1_0_0"/>
    <dbReference type="OMA" id="GWLGKPH"/>
    <dbReference type="OrthoDB" id="724641at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="36"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACJ75173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="352" mass="41141" checksum="6729AD0B6F7E2FDF" modified="2009-02-10" version="1">MLFYVAFLIISLYFLLNKNNALLKSNKKLGSNYKISVIIPARNEEKNIEKILNSLRNQTLKVHEIIVVDDASEDNTAKVVEKFQEVKLIQLEDSPKQGWNGKSWAIWNGYLNSSGELLLFVDADVELEREAIETLVEKFNKYKGLISVWPYQRFEKFHEHFTLVFNLVIIYLSNLIGTFSRKPKGAFGPVILTSRKDYEFVGGHKAIRDSVLDDIKLGKLYSKSNINVKNFLGGRYIKFRMYPQNLRQLYEGFSKNMASGSISGGFFNLIIAILWTFGIFSALFTFDRYFVYRYFSVVVFLYLLSKNLGDYKWYDYIFFPIHLLFFAVTFFNSLYRKLFLHSVVWKGRKINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGH8</accession>
    <name>B7IGH8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_724</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004104650.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_724"/>
    <dbReference type="EnsemblBacteria" id="ACJ75192">
        <property type="protein sequence ID" value="ACJ75192"/>
        <property type="gene ID" value="THA_724"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_724"/>
    <dbReference type="eggNOG" id="COG0432">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096980_1_1_0"/>
    <dbReference type="OMA" id="VRHTSCS"/>
    <dbReference type="OrthoDB" id="1691156at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="PTHR30615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="SSF111038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01314">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="132" mass="14732" checksum="6058D340F4FCF5F2" modified="2009-02-10" version="1">MLYSLEVPTKTRNEFIDITHKIEETISKSGIKSGICVVFVPHTTAAVTINENADPSVKSDIVKTLNKVIPANWDYTHIEGNSDSHIKSTLVSPSITLIIENGKLVLGTWQGVYFCEFDGPRRRKVFIKILSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGQ8</accession>
    <name>B7IGQ8_THEAB</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flagellar basal-body rod protein FlgG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Distal rod protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">flgG</name>
        <name evidence="8" type="ordered locus">THA_810</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">TCF52B</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod. The rod consists of about 26 subunits of FlgG in the distal portion, and FlgB, FlgC and FlgF are thought to build up the proximal portion of the rod with about 6 subunits each.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579812.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_810"/>
    <dbReference type="EnsemblBacteria" id="ACJ75272">
        <property type="protein sequence ID" value="ACJ75272"/>
        <property type="gene ID" value="THA_810"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_810"/>
    <dbReference type="eggNOG" id="COG4786">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013687_0_1_0"/>
    <dbReference type="OMA" id="TRDGTFH"/>
    <dbReference type="OrthoDB" id="1488472at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009426">
        <property type="term" value="C:bacterial-type flagellum basal body, distal rod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019776">
        <property type="entry name" value="Flagellar_basal_body_rod_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020013">
        <property type="entry name" value="Flagellar_FlgE/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037925">
        <property type="entry name" value="FlgE/F/G-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012836">
        <property type="entry name" value="FlgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012834">
        <property type="entry name" value="FlgG_G_neg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117143">
        <property type="entry name" value="SSF117143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03506">
        <property type="entry name" value="FlgEFG_subfam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02490">
        <property type="entry name" value="flgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02488">
        <property type="entry name" value="flgG_G_neg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00588">
        <property type="entry name" value="FLAGELLA_BB_ROD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="6">
        <location>
            <begin position="5"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="7">
        <location>
            <begin position="182"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ACJ75272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="261" mass="28026" checksum="52E0C316DE1526E3" modified="2009-02-10" version="1">MINGLYTAATGMWAQQFKLDTLSNNIANVDTAGYKKVKSEFQDLLYDYSKNAGAATAQNSLHPTGLYVGHGTKLSATTRIFTQGNLERTGNSLDLAISGDGFFQIQLQDGRIAYTRDGQFKIDGNGRIITSNGNLLSPNIVVPQNAVSLSISPDGIVTAELQDGTLQNLGTITLVRFINPSGLKAIGDNLFLETPASGTPVEGTAGQDGFGSILQGYVEKSNVDIVKEMVDMISAMRAYELNSRTIQTADEMLRTASSMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGT8</accession>
    <name>B7IGT8_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Membrane associated methyl-accepting chemotaxis protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">THA_841</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004100460.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_841"/>
    <dbReference type="EnsemblBacteria" id="ACJ75302">
        <property type="protein sequence ID" value="ACJ75302"/>
        <property type="gene ID" value="THA_841"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_841"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_415562_0_0_0"/>
    <dbReference type="OMA" id="RIENNIT"/>
    <dbReference type="OrthoDB" id="477199at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011623">
        <property type="entry name" value="7TMR_DISM_rcpt_extracell_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07695">
        <property type="entry name" value="7TMR-DISM_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="416"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="612"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ACJ75302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="660" mass="75540" checksum="5D8FFD7D45D356AE" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>B7IGY9</accession>
    <name>B7IGY9_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_894</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012579868.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_894"/>
    <dbReference type="EnsemblBacteria" id="ACJ75353">
        <property type="protein sequence ID" value="ACJ75353"/>
        <property type="gene ID" value="THA_894"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_894"/>
    <dbReference type="eggNOG" id="ENOG502ZK8Y">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195306_0_0_0"/>
    <dbReference type="OMA" id="WSIVKLF"/>
    <dbReference type="OrthoDB" id="2000748at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="63" mass="7352" checksum="DD3BF0CD95311EFA" modified="2009-02-10" version="1">MIWSIVKLFMSLVERPGDGEKKKKEVIELVDSIFDYLHVDIDREDLELVLSPAIDYLANILFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHE1</accession>
    <name>B7IHE1_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">THA_1047</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1047"/>
    <dbReference type="EnsemblBacteria" id="ACJ75505">
        <property type="protein sequence ID" value="ACJ75505"/>
        <property type="gene ID" value="THA_1047"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1047"/>
    <dbReference type="eggNOG" id="COG1833">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2959339_0_0_0"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ACJ75505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="59" mass="6784" checksum="D9B3E1FF02DE07E8" modified="2009-02-10" version="1">MLSSKTKCEEKISLYMSQFFTGPKGFGSSDLKVQTNLYYIDNFDNFSKILSNIFKGELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>B7IHP7</accession>
    <name>B7IHP7_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methyl-accepting chemotaxis protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">THA_1159</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">TCF52B</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012580047.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1159"/>
    <dbReference type="EnsemblBacteria" id="ACJ75611">
        <property type="protein sequence ID" value="ACJ75611"/>
        <property type="gene ID" value="THA_1159"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1159"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_19_0"/>
    <dbReference type="OMA" id="VMSEITH"/>
    <dbReference type="OrthoDB" id="477199at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029151">
        <property type="entry name" value="Sensor-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103190">
        <property type="entry name" value="SSF103190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="6">
        <location>
            <begin position="293"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="364"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="414"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="603"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACJ75611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="652" mass="72030" checksum="57CA6CC288466364" modified="2009-02-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-02-10" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>B7II05</accession>
    <name>B7II05_THEAB</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycoprotease family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">THA_1274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermosipho africanus (strain TCF52B)</name>
        <dbReference type="NCBI Taxonomy" id="484019"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Thermotogae</taxon>
            <taxon>Thermotogales</taxon>
            <taxon>Fervidobacteriaceae</taxon>
            <taxon>Thermosipho</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1974" last="1978">
            <title>The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea.</title>
            <authorList>
                <person name="Nesboe C.L."/>
                <person name="Bapteste E."/>
                <person name="Curtis B."/>
                <person name="Dahle H."/>
                <person name="Lopez P."/>
                <person name="Macleod D."/>
                <person name="Dlutek M."/>
                <person name="Bowman S."/>
                <person name="Zhaxybayeva O."/>
                <person name="Birkeland N.-K."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="19124572"/>
            <dbReference type="DOI" id="10.1128/JB.01448-08"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">TCF52B</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP001185">
        <property type="protein sequence ID" value="ACJ75719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004101548.1">
        <property type="nucleotide sequence ID" value="NC_011653.1"/>
    </dbReference>
    <dbReference type="STRING" id="484019.THA_1274"/>
    <dbReference type="EnsemblBacteria" id="ACJ75719">
        <property type="protein sequence ID" value="ACJ75719"/>
        <property type="gene ID" value="THA_1274"/>
    </dbReference>
    <dbReference type="KEGG" id="taf:THA_1274"/>
    <dbReference type="eggNOG" id="COG1214">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064886_0_0_0"/>
    <dbReference type="OMA" id="ELNWERK"/>
    <dbReference type="OrthoDB" id="1826274at2"/>
    <dbReference type="Proteomes" id="UP000002453">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022496">
        <property type="entry name" value="T6A_TsaB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF11">
        <property type="entry name" value="PTHR11735:SF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03725">
        <property type="entry name" value="T6A_YeaZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TsaD" evidence="1">
        <location>
            <begin position="33"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACJ75719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002453"/>
        </source>
    </evidence>
    <sequence length="216" mass="24322" checksum="C29E7A4010261D93" modified="2009-02-10" version="1">MKILALDTSTSKIVVYYRDSEKIITQTYMGKDKHGRNLGVVMNNLKNFNINFEDIDVVGIGIGPGSLTGLRVGISFALGLSIDKKIVVVPSTKLIAANLMNSGKNIVVTRKARQGYIYGAVYDEYLNNIVEPFVESIENFSKEFEKMENFVVIGDGAEYFGGNLPEIYNYPEPSRLGYLVEKEIEVENFVEKVEPLYIQKSIAEINFEKKKQRKDG</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>